Henri Joyeux - Academia.edu (original) (raw)

Papers by Henri Joyeux

Research paper thumbnail of Rôle protecteur d'un apport oral de sélénium et de vitamine E vis-à-vis de la cardiotoxicité induite par les anthracyclines chez le rat

Nutrition Clinique et Métabolisme, 1996

Research paper thumbnail of Dénutrition et immunité en cancérologie chirurgicale : Modèles biologiques cliniques et expérimentaux

Nutrition Clinique et Métabolisme, 1993

Research paper thumbnail of Application de l'infographie tridimensionnelle en cancérologie. Discussion

Research paper thumbnail of <title>Advanced 3D image processing techniques for liver and hepatic tumor location and volumetry</title>

Medical Imaging 1999: Image Processing, 1999

To assist radiologists and physicians in diaposing, and in treatment planning and evaluating in l... more To assist radiologists and physicians in diaposing, and in treatment planning and evaluating in liver oncology, we have developed a fast and accurate segmentation of the liver and its lesions within CT-scan exams . The first step of our method is to reduce spatial resolution of CT images . This will have two effects : obtain near isotropic 3D data space and drastically decrease computational time for further processing . On a second step a 3D non-linear "edge-preserving" smoothing filtering is performed throughout the entire exam .On a third step the 3D regions coming out from the second step are homogeneous enough to allow a quite simple segmentation process. based on morphological operatious, under supervisor control, ending up with accurate 3D regions of interest (ROI) of the liver and al1 the hepatic tumors . On a fourth step the ROIS are eventually set back into the original images, features like volume and location are immediately computed and displayed . The segmentation we get is as precise as a manual one but is much faster .

Research paper thumbnail of Characterisation of a newly isolated Caco-2 clone (TC-7), as a model of transport processes and biotransformation of drugs

International Journal of Pharmaceutics, 1995

... Isabelle Caro a, Xavier Boulenc a Monique Rousset b Viviane Meunier a Martine Bourri6 a, Bern... more ... Isabelle Caro a, Xavier Boulenc a Monique Rousset b Viviane Meunier a Martine Bourri6 a, Bernand Julian a, Henri Joyeux c, Claude Roques a ... Cell culture Parental Caco-2 cells, originating from a hu-man colorectal carcinoma (Fogh et al., 1977), were obtained from Dr J. Fogh ...

Research paper thumbnail of Advanced 3D image processing techniques for liver and hepatic tumor location and volumetry

To assist radiologists and physicians in diagnosing, and in treatment planning and evaluating in ... more To assist radiologists and physicians in diagnosing, and in treatment planning and evaluating in liver oncology, we have developed a fast and accurate segmentation of the liver and its lesions within CT-scan exams. The first step of our method is to reduce spatial resolution of CT images. This will have two effects: obtain near isotropic 3D data space and drastically decrease computational time for further processing. On a second step a 3D non-linear `edge- preserving' smoothing filtering is performed throughout the entire exam. On a third step the 3D regions coming out from the second step are homogeneous enough to allow a quite simple segmentation process, based on morphological operations, under supervisor control, ending up with accurate 3D regions of interest (ROI) of the liver and all the hepatic tumors. On a fourth step the ROIs are eventually set back into the original images, features like volume and location are immediately computed and displayed. The segmentation we ...

Research paper thumbnail of Internal Jugular Vein Cannulation Using 2 Silastic Catheters

Nephron, 1986

Subclavian vein cannulation, although a major progress in temporary vascular access, was associat... more Subclavian vein cannulation, although a major progress in temporary vascular access, was associated with a significant morbidity and mortality. A safer alternative technique would therefore be welcome. For the last 2 years, we developed a new approach consisting in internal jugular vein cannulation (IJVC) with 2 silicone rubber catheters with a long-term proved biocompatibility. 138 pairs of cannulae were inserted in 129 uremics patients. Mean duration of use was 54 days (1 day to 17 months). More than 3,000 high-performance HD sessions were performed. IJVC handling and care was easily managed by nurses. Low incidence of complications was observed: 2 thrombosis episodes, 3 skin infection with 2 septicemiae, 1 air embolism, 1 anaphylactoid reaction. No death was related to the technique itself. IJVC offers a new method of percutaneous central vein cannulation, providing a safe and reliable temporary vascular access immediately usable for all kinds of extracorporeal treatment.

Research paper thumbnail of Home enteral nutrition system: one patient, one daily ration of an "all-in-one" sterile and modular formula in a single container

Journal of Parenteral and Enteral Nutrition, 1990

A home enteral nutrition (HEN) system was introduced to deliver a day&amp;amp;#39;s ration of... more A home enteral nutrition (HEN) system was introduced to deliver a day&amp;amp;#39;s ration of an &amp;amp;quot;all-in-one&amp;amp;quot; sterile and modular formula from a large container. The nutrient formula consisted of carbohydrate, protein, and lipid modules sterilized separately, in an autoclave at 120 degrees C, then mixed and supplemented with minerals and trace elements. Vitamins were given the patients through a feeding tube before connecting the nutrient container. The system was used to feed 12 malnourished patients (10 men and two women) with cancer (carcinoma of esophagus or tongue) or without cancer (esophageal perforation). All patients had normal gastrointestinal function but could not take in more than 500 kcal/day orally, because of their primary disease or the treatment for it (surgery in four patients; and radiotherapy, associated in some cases with chemotherapy, in eight). HEN duration ranged from 30 to 435 days at a daily cost of approximately US 15to15 to 15to20. Statistical analysis (Student&amp;amp;#39;s t-test for paired data) showed significant body weight gain (p less than 0.05) and significant increases in serum levels of albumin, transthyretin (p less than 0.05, respectively), and transferrin (p less than 0.01). Increases of serum total protein level were not statistically significant (p less than 0.09). The nutrient formula was well tolerated and there were no HEN-related rehospitalizations. Seventy-five % (nine of the patients) considered that HEN had improved their quality of life. The daily savings per patient, compared to the cost of treatment in a hospital, was about $260/day.(ABSTRACT TRUNCATED AT 250 WORDS)

Research paper thumbnail of Lingual fibrosarcoma induced by 7,12-dimethylbenzanthracene in the rat

Journal of Oral Pathology and Medicine, 1990

A single injection of 5 mg of 7, 12-dimethylbenzanthracene (DMBA) resulted in a high incidence of... more A single injection of 5 mg of 7, 12-dimethylbenzanthracene (DMBA) resulted in a high incidence of lingual fibrosarcoma in 67 Wistar rats. No metastases were found. We studied the influence of age and sex on the incidence, latency of tumors, loss of weight and the spontaneous survival of animals. The method described offers an experimental model for the study of cachexia resulting from upper digestive and respiratory tract cancer.

Research paper thumbnail of Antiproliferative effect of FK508 and cyclosporine a on adult human hepatocytes in primary culture

Journal of Hepatology, 1991

Research paper thumbnail of Mesure semi-automatique des différents lobes hépatiques. Application à la recherche d’une corrélation entre volumes des lobes du foie. Une étude sur 50 angioscanners de foies sains

Annales de Chirurgie, 2003

Objective. – Healthy and pathological hepatic volumetry can be of great interest in Liver patholo... more Objective. – Healthy and pathological hepatic volumetry can be of great interest in Liver pathology and treatment. We studied the hepatic segmentation in order to verify if it is possible to estimate the volume of one lobe, when the volume of the other lobe or the total liver is known.Patients and Methods. – We studied 50 normal hepatic angiotomographies. For each exam and using the Couinaud classification, we measured the total hepatic volume, the right lobe, the left lobe and the caudate lobe volumes. We used a software called “Hepato” developed in order to quantify automatically CTScan images of the liver. We performed a linear regression analysis (least squares method) and calculated the determination coefficient to study the correlation between the different volumes.Results. – The mean total hepatic volume was 1497 cm3. We obtained for the right lobe 1231 cm3, 226 cm3 for left lobe, and 39 cm3 for the caudate lobe. Moreover there was a weak correlation between these volumes.Conclusion. – Exact volume estimation of an hepatic lobe in one patient can not be obtained from the total hepatic volume. However, theses volumes can only be measured from three-dimensional images. Since classical image editing tools are time request, it becomes impossible to utilize them in a clinical routine to get the different volumes of the Liver. Thus, a software dedicated for liver parenchyma recognition is necessary.

Research paper thumbnail of Hepatic Assistance After Subtotal or Total Hepatectomy in the Dog

Research paper thumbnail of Internal Jugular Vein Cannulation with Two Silicone Rubber Catheters: A New and Safe Temporary Vascular Access for Hemodialysis. Thirty Months' Experience

Artificial Organs, 1986

Temporary and immediately usable vascular access is a vital need in treating uremic patients. Sub... more Temporary and immediately usable vascular access is a vital need in treating uremic patients. Subclavian vein cannulation, although a major progress, has been associated with significant morbidity and mortality. Accordingly, for the last 2.5 years the authors have been developing an alternative technique consisting of internal jugular vein cannulation (IJVC) with two silicone rubber catheters with a long-term, proved biocompatibility. One hundred sixty-five pairs of cannulas were inserted in 153 patients. Mean duration of use was 57 days (1 day to 17 months). More than 3,000 high-performance hemodialysis sessions were performed. IJVC handling and care were easily managed by nurses. A low incidence of complications was observed: two thrombosis episodes, four skin infections with three septicemia, one air embolism, and one anaphylactoid reaction. No death was related to the technique itself. IJVC offers a new, safe, and reliable temporary vascular access immediately usable for all kinds of extracorporeal treatment.

Research paper thumbnail of Plasma and jejunal glutamine levels after extensive small bowel resection in dogs

Clinical Nutrition, 1992

Recent studies have demonstrated that glutamine may be required for mucosal growth and that it is... more Recent studies have demonstrated that glutamine may be required for mucosal growth and that it is widely utilised by the intestinal tract after surgery. This study has evaluated the effect of massive small bowel resection on plasma and jejunal glutamine and related amino-acids level evolution after surgery. Transection was performed in 6 dogs (control group) and enterectomy in 10 dogs leaving 25cm of jejunum, associated with colectomy (group 1). Plasma glutamine levels decreased on D2 (p = 0.03) in the resected group while a significant decrease of plasma alanine levels was observed on D2 (p = 0.002), D4, D6 and D8 (p < 0.001). Intestinal mucosa glutamic acid content was increased on 08 in this group (p < 0.001). No changes were observed in the control group. These results suggest that glutamine is a required substrate after massive small bowel resection which could improve the intestinal adaptation encountered after enterectomy.

Research paper thumbnail of Influence of N-acetylglutamine or glutamine infusion on plasma amino acid concentrations during the early phase of small-bowel adaptation in the dog

Journal of Parenteral and Enteral Nutrition, 1992

Glutamine is a nonessential neutral amino acid that is widely consumed by the intestinal tract in... more Glutamine is a nonessential neutral amino acid that is widely consumed by the intestinal tract in catabolic states. We have followed up the plasma amino acid profile after extensive small-bowel resection in dogs receiving total parenteral nutrition (TPN) with or without glutamine (GLN) or N-acetylglutamine (aGLN) supplementation. Animals were divided into four groups according to the type of surgery (enterectomy or transection) and nutrition (TPN, TPN with aGLN, or TPN with GLN). Plasma GLN levels decreased in group I (enterectomy and TPN) on day 2 (p = .03) and significantly increased on postoperative days in groups III (enterectomy and TPN with aGLN) and IV (enterectomy and TPN with GLN). A significant increase of plasma GLN was observed in groups III and IV compared with group I on days 6 and 8 (p = .03 and p = .01). Plasma alanine decreased in groups with bowel resection, whereas no change was observed in the control group (transection) and the decrease of plasma alanine was significantly less pronounced in groups III and IV compared with group I. The increase of crypt depth and villous height was more pronounced in groups III and IV. These results suggest that GLN is a required substrate for mucosal growth and function, which could improve the intestinal adaptation encountered after enterectomy.

Research paper thumbnail of A drug adjunct for postoperative external alimentary tract fistulas

American journal of surgery, 1994

Research paper thumbnail of Human Hepatocytes in Primary Culture

The expression of several forms of cytochrome P450 including P450 1A2, 2D6, 2E1, and 3A was inves... more The expression of several forms of cytochrome P450 including P450 1A2, 2D6, 2E1, and 3A was investigated in human hepatocytes maintained in primary culture for 96 h in the absence or presence of 50 sM of various imidazole derivatives. These included ketoconazole, clotrimazole, miconazole, fluconazole, sec- nidazole and metronidazole. In addition, the typical in- ducers rifampicin and 3-naphthoflavone were used

Research paper thumbnail of Bisphosphonates increase tight junction permeability in the human intestinal epithelial (Caco-2) model

International Journal of Pharmaceutics, 1995

The human Caco-2 monolayer model was used to elucidate the potential role of bisphosphonates in t... more The human Caco-2 monolayer model was used to elucidate the potential role of bisphosphonates in the regulation of the junctional complex opening. Experiments were performed in parallel with EGTA, a potent free Ca 2+ chelator, which was already demonstrated to increase intestinal epithelium permeability both in vitro and in vivo. Following treatment of Caco-2 cells with increasing non-toxic concentrations of tiludronate, a new bisphosphohate, different phenomena could be observed such as (i) free Ca 2 ÷ complex formation at tiludronate concentrations above 0.3 mM and a Ca 2÷ ICs0 around 10-20 mM, (ii) increase in monolayer permeability, i.e., decrease in the transmembrane epithelial electrical resistance, at tiludronate concentrations above 10 mM and a TEER IC50 around 40 raM, associated with (iii) an opening of the junctional complex (desmosomes, intermediate and tight junctions) as demonstrated by scanning electron microscopy of the cell monolayer and electron microscopy of the apical pole of cells. (iv) The direct consequence of these observations was an increase in monolayer permeability at the level of the paracellular route. Hence, the paracellular transport not only of the PEG400 probe was increased following treatment of Caco-2 monolayers with tiludronate concentrations above 20 mM but also that of tiludronate itself at concentrations above 15 mM. These results are in agreement with a previous study (Boulenc et al., Biochem. Pharmacol., 46 (1993) 1591-1600 which showed that tiludronate was specifically transported across Caco-2 cells via the paracellular route. Therefore, by its specific effect on free Ca 2+ concentration, tiludronate could regulate the junctional complex opening of the intestinal epithelium monolayer and enhance its own transport across the intestinal wall.

Research paper thumbnail of Testosterone secretion is severely impaired after hepatectomy in rat, dog and man

Journal of Steroid Biochemistry, 1985

In 90%-hepatectomized rats, the plasma testosterone level (0.34 +/- 0.07 ng.ml, mean +/- SEM) is ... more In 90%-hepatectomized rats, the plasma testosterone level (0.34 +/- 0.07 ng.ml, mean +/- SEM) is significantly lower (P less than 0.001) than in sham operated male rats (1.7 +/- 0.26 ng.ml, mean +/- SEM). In dogs, after 90% hepatectomy, the mean plasma testosterone concentration fell to 1/10 of the plasma testosterone level measured in sham operated animals either 24 or 72 h after surgery. In hepatectomized men, plasma testosterone is markedly decreased in contrast to what is observed after duodeno-pancreatectomy performed under the same conditions of anesthesia. These results suggest that 90% hepatectomy severely alters the Leydig cell function.

Research paper thumbnail of Characterisation of a newly isolated Caco-2 clone (TC-7), as a model of transport processes and biotransformation of drugs

International Journal of Pharmaceutics, 1995

... Isabelle Caro a, Xavier Boulenc a Monique Rousset b Viviane Meunier a Martine Bourri6 a, Bern... more ... Isabelle Caro a, Xavier Boulenc a Monique Rousset b Viviane Meunier a Martine Bourri6 a, Bernand Julian a, Henri Joyeux c, Claude Roques a ... Cell culture Parental Caco-2 cells, originating from a hu-man colorectal carcinoma (Fogh et al., 1977), were obtained from Dr J. Fogh ...

Research paper thumbnail of Rôle protecteur d'un apport oral de sélénium et de vitamine E vis-à-vis de la cardiotoxicité induite par les anthracyclines chez le rat

Nutrition Clinique et Métabolisme, 1996

Research paper thumbnail of Dénutrition et immunité en cancérologie chirurgicale : Modèles biologiques cliniques et expérimentaux

Nutrition Clinique et Métabolisme, 1993

Research paper thumbnail of Application de l'infographie tridimensionnelle en cancérologie. Discussion

Research paper thumbnail of <title>Advanced 3D image processing techniques for liver and hepatic tumor location and volumetry</title>

Medical Imaging 1999: Image Processing, 1999

To assist radiologists and physicians in diaposing, and in treatment planning and evaluating in l... more To assist radiologists and physicians in diaposing, and in treatment planning and evaluating in liver oncology, we have developed a fast and accurate segmentation of the liver and its lesions within CT-scan exams . The first step of our method is to reduce spatial resolution of CT images . This will have two effects : obtain near isotropic 3D data space and drastically decrease computational time for further processing . On a second step a 3D non-linear "edge-preserving" smoothing filtering is performed throughout the entire exam .On a third step the 3D regions coming out from the second step are homogeneous enough to allow a quite simple segmentation process. based on morphological operatious, under supervisor control, ending up with accurate 3D regions of interest (ROI) of the liver and al1 the hepatic tumors . On a fourth step the ROIS are eventually set back into the original images, features like volume and location are immediately computed and displayed . The segmentation we get is as precise as a manual one but is much faster .

Research paper thumbnail of Characterisation of a newly isolated Caco-2 clone (TC-7), as a model of transport processes and biotransformation of drugs

International Journal of Pharmaceutics, 1995

... Isabelle Caro a, Xavier Boulenc a Monique Rousset b Viviane Meunier a Martine Bourri6 a, Bern... more ... Isabelle Caro a, Xavier Boulenc a Monique Rousset b Viviane Meunier a Martine Bourri6 a, Bernand Julian a, Henri Joyeux c, Claude Roques a ... Cell culture Parental Caco-2 cells, originating from a hu-man colorectal carcinoma (Fogh et al., 1977), were obtained from Dr J. Fogh ...

Research paper thumbnail of Advanced 3D image processing techniques for liver and hepatic tumor location and volumetry

To assist radiologists and physicians in diagnosing, and in treatment planning and evaluating in ... more To assist radiologists and physicians in diagnosing, and in treatment planning and evaluating in liver oncology, we have developed a fast and accurate segmentation of the liver and its lesions within CT-scan exams. The first step of our method is to reduce spatial resolution of CT images. This will have two effects: obtain near isotropic 3D data space and drastically decrease computational time for further processing. On a second step a 3D non-linear `edge- preserving' smoothing filtering is performed throughout the entire exam. On a third step the 3D regions coming out from the second step are homogeneous enough to allow a quite simple segmentation process, based on morphological operations, under supervisor control, ending up with accurate 3D regions of interest (ROI) of the liver and all the hepatic tumors. On a fourth step the ROIs are eventually set back into the original images, features like volume and location are immediately computed and displayed. The segmentation we ...

Research paper thumbnail of Internal Jugular Vein Cannulation Using 2 Silastic Catheters

Nephron, 1986

Subclavian vein cannulation, although a major progress in temporary vascular access, was associat... more Subclavian vein cannulation, although a major progress in temporary vascular access, was associated with a significant morbidity and mortality. A safer alternative technique would therefore be welcome. For the last 2 years, we developed a new approach consisting in internal jugular vein cannulation (IJVC) with 2 silicone rubber catheters with a long-term proved biocompatibility. 138 pairs of cannulae were inserted in 129 uremics patients. Mean duration of use was 54 days (1 day to 17 months). More than 3,000 high-performance HD sessions were performed. IJVC handling and care was easily managed by nurses. Low incidence of complications was observed: 2 thrombosis episodes, 3 skin infection with 2 septicemiae, 1 air embolism, 1 anaphylactoid reaction. No death was related to the technique itself. IJVC offers a new method of percutaneous central vein cannulation, providing a safe and reliable temporary vascular access immediately usable for all kinds of extracorporeal treatment.

Research paper thumbnail of Home enteral nutrition system: one patient, one daily ration of an "all-in-one" sterile and modular formula in a single container

Journal of Parenteral and Enteral Nutrition, 1990

A home enteral nutrition (HEN) system was introduced to deliver a day&amp;amp;#39;s ration of... more A home enteral nutrition (HEN) system was introduced to deliver a day&amp;amp;#39;s ration of an &amp;amp;quot;all-in-one&amp;amp;quot; sterile and modular formula from a large container. The nutrient formula consisted of carbohydrate, protein, and lipid modules sterilized separately, in an autoclave at 120 degrees C, then mixed and supplemented with minerals and trace elements. Vitamins were given the patients through a feeding tube before connecting the nutrient container. The system was used to feed 12 malnourished patients (10 men and two women) with cancer (carcinoma of esophagus or tongue) or without cancer (esophageal perforation). All patients had normal gastrointestinal function but could not take in more than 500 kcal/day orally, because of their primary disease or the treatment for it (surgery in four patients; and radiotherapy, associated in some cases with chemotherapy, in eight). HEN duration ranged from 30 to 435 days at a daily cost of approximately US 15to15 to 15to20. Statistical analysis (Student&amp;amp;#39;s t-test for paired data) showed significant body weight gain (p less than 0.05) and significant increases in serum levels of albumin, transthyretin (p less than 0.05, respectively), and transferrin (p less than 0.01). Increases of serum total protein level were not statistically significant (p less than 0.09). The nutrient formula was well tolerated and there were no HEN-related rehospitalizations. Seventy-five % (nine of the patients) considered that HEN had improved their quality of life. The daily savings per patient, compared to the cost of treatment in a hospital, was about $260/day.(ABSTRACT TRUNCATED AT 250 WORDS)

Research paper thumbnail of Lingual fibrosarcoma induced by 7,12-dimethylbenzanthracene in the rat

Journal of Oral Pathology and Medicine, 1990

A single injection of 5 mg of 7, 12-dimethylbenzanthracene (DMBA) resulted in a high incidence of... more A single injection of 5 mg of 7, 12-dimethylbenzanthracene (DMBA) resulted in a high incidence of lingual fibrosarcoma in 67 Wistar rats. No metastases were found. We studied the influence of age and sex on the incidence, latency of tumors, loss of weight and the spontaneous survival of animals. The method described offers an experimental model for the study of cachexia resulting from upper digestive and respiratory tract cancer.

Research paper thumbnail of Antiproliferative effect of FK508 and cyclosporine a on adult human hepatocytes in primary culture

Journal of Hepatology, 1991

Research paper thumbnail of Mesure semi-automatique des différents lobes hépatiques. Application à la recherche d’une corrélation entre volumes des lobes du foie. Une étude sur 50 angioscanners de foies sains

Annales de Chirurgie, 2003

Objective. – Healthy and pathological hepatic volumetry can be of great interest in Liver patholo... more Objective. – Healthy and pathological hepatic volumetry can be of great interest in Liver pathology and treatment. We studied the hepatic segmentation in order to verify if it is possible to estimate the volume of one lobe, when the volume of the other lobe or the total liver is known.Patients and Methods. – We studied 50 normal hepatic angiotomographies. For each exam and using the Couinaud classification, we measured the total hepatic volume, the right lobe, the left lobe and the caudate lobe volumes. We used a software called “Hepato” developed in order to quantify automatically CTScan images of the liver. We performed a linear regression analysis (least squares method) and calculated the determination coefficient to study the correlation between the different volumes.Results. – The mean total hepatic volume was 1497 cm3. We obtained for the right lobe 1231 cm3, 226 cm3 for left lobe, and 39 cm3 for the caudate lobe. Moreover there was a weak correlation between these volumes.Conclusion. – Exact volume estimation of an hepatic lobe in one patient can not be obtained from the total hepatic volume. However, theses volumes can only be measured from three-dimensional images. Since classical image editing tools are time request, it becomes impossible to utilize them in a clinical routine to get the different volumes of the Liver. Thus, a software dedicated for liver parenchyma recognition is necessary.

Research paper thumbnail of Hepatic Assistance After Subtotal or Total Hepatectomy in the Dog

Research paper thumbnail of Internal Jugular Vein Cannulation with Two Silicone Rubber Catheters: A New and Safe Temporary Vascular Access for Hemodialysis. Thirty Months' Experience

Artificial Organs, 1986

Temporary and immediately usable vascular access is a vital need in treating uremic patients. Sub... more Temporary and immediately usable vascular access is a vital need in treating uremic patients. Subclavian vein cannulation, although a major progress, has been associated with significant morbidity and mortality. Accordingly, for the last 2.5 years the authors have been developing an alternative technique consisting of internal jugular vein cannulation (IJVC) with two silicone rubber catheters with a long-term, proved biocompatibility. One hundred sixty-five pairs of cannulas were inserted in 153 patients. Mean duration of use was 57 days (1 day to 17 months). More than 3,000 high-performance hemodialysis sessions were performed. IJVC handling and care were easily managed by nurses. A low incidence of complications was observed: two thrombosis episodes, four skin infections with three septicemia, one air embolism, and one anaphylactoid reaction. No death was related to the technique itself. IJVC offers a new, safe, and reliable temporary vascular access immediately usable for all kinds of extracorporeal treatment.

Research paper thumbnail of Plasma and jejunal glutamine levels after extensive small bowel resection in dogs

Clinical Nutrition, 1992

Recent studies have demonstrated that glutamine may be required for mucosal growth and that it is... more Recent studies have demonstrated that glutamine may be required for mucosal growth and that it is widely utilised by the intestinal tract after surgery. This study has evaluated the effect of massive small bowel resection on plasma and jejunal glutamine and related amino-acids level evolution after surgery. Transection was performed in 6 dogs (control group) and enterectomy in 10 dogs leaving 25cm of jejunum, associated with colectomy (group 1). Plasma glutamine levels decreased on D2 (p = 0.03) in the resected group while a significant decrease of plasma alanine levels was observed on D2 (p = 0.002), D4, D6 and D8 (p < 0.001). Intestinal mucosa glutamic acid content was increased on 08 in this group (p < 0.001). No changes were observed in the control group. These results suggest that glutamine is a required substrate after massive small bowel resection which could improve the intestinal adaptation encountered after enterectomy.

Research paper thumbnail of Influence of N-acetylglutamine or glutamine infusion on plasma amino acid concentrations during the early phase of small-bowel adaptation in the dog

Journal of Parenteral and Enteral Nutrition, 1992

Glutamine is a nonessential neutral amino acid that is widely consumed by the intestinal tract in... more Glutamine is a nonessential neutral amino acid that is widely consumed by the intestinal tract in catabolic states. We have followed up the plasma amino acid profile after extensive small-bowel resection in dogs receiving total parenteral nutrition (TPN) with or without glutamine (GLN) or N-acetylglutamine (aGLN) supplementation. Animals were divided into four groups according to the type of surgery (enterectomy or transection) and nutrition (TPN, TPN with aGLN, or TPN with GLN). Plasma GLN levels decreased in group I (enterectomy and TPN) on day 2 (p = .03) and significantly increased on postoperative days in groups III (enterectomy and TPN with aGLN) and IV (enterectomy and TPN with GLN). A significant increase of plasma GLN was observed in groups III and IV compared with group I on days 6 and 8 (p = .03 and p = .01). Plasma alanine decreased in groups with bowel resection, whereas no change was observed in the control group (transection) and the decrease of plasma alanine was significantly less pronounced in groups III and IV compared with group I. The increase of crypt depth and villous height was more pronounced in groups III and IV. These results suggest that GLN is a required substrate for mucosal growth and function, which could improve the intestinal adaptation encountered after enterectomy.

Research paper thumbnail of A drug adjunct for postoperative external alimentary tract fistulas

American journal of surgery, 1994

Research paper thumbnail of Human Hepatocytes in Primary Culture

The expression of several forms of cytochrome P450 including P450 1A2, 2D6, 2E1, and 3A was inves... more The expression of several forms of cytochrome P450 including P450 1A2, 2D6, 2E1, and 3A was investigated in human hepatocytes maintained in primary culture for 96 h in the absence or presence of 50 sM of various imidazole derivatives. These included ketoconazole, clotrimazole, miconazole, fluconazole, sec- nidazole and metronidazole. In addition, the typical in- ducers rifampicin and 3-naphthoflavone were used

Research paper thumbnail of Bisphosphonates increase tight junction permeability in the human intestinal epithelial (Caco-2) model

International Journal of Pharmaceutics, 1995

The human Caco-2 monolayer model was used to elucidate the potential role of bisphosphonates in t... more The human Caco-2 monolayer model was used to elucidate the potential role of bisphosphonates in the regulation of the junctional complex opening. Experiments were performed in parallel with EGTA, a potent free Ca 2+ chelator, which was already demonstrated to increase intestinal epithelium permeability both in vitro and in vivo. Following treatment of Caco-2 cells with increasing non-toxic concentrations of tiludronate, a new bisphosphohate, different phenomena could be observed such as (i) free Ca 2 ÷ complex formation at tiludronate concentrations above 0.3 mM and a Ca 2÷ ICs0 around 10-20 mM, (ii) increase in monolayer permeability, i.e., decrease in the transmembrane epithelial electrical resistance, at tiludronate concentrations above 10 mM and a TEER IC50 around 40 raM, associated with (iii) an opening of the junctional complex (desmosomes, intermediate and tight junctions) as demonstrated by scanning electron microscopy of the cell monolayer and electron microscopy of the apical pole of cells. (iv) The direct consequence of these observations was an increase in monolayer permeability at the level of the paracellular route. Hence, the paracellular transport not only of the PEG400 probe was increased following treatment of Caco-2 monolayers with tiludronate concentrations above 20 mM but also that of tiludronate itself at concentrations above 15 mM. These results are in agreement with a previous study (Boulenc et al., Biochem. Pharmacol., 46 (1993) 1591-1600 which showed that tiludronate was specifically transported across Caco-2 cells via the paracellular route. Therefore, by its specific effect on free Ca 2+ concentration, tiludronate could regulate the junctional complex opening of the intestinal epithelium monolayer and enhance its own transport across the intestinal wall.

Research paper thumbnail of Testosterone secretion is severely impaired after hepatectomy in rat, dog and man

Journal of Steroid Biochemistry, 1985

In 90%-hepatectomized rats, the plasma testosterone level (0.34 +/- 0.07 ng.ml, mean +/- SEM) is ... more In 90%-hepatectomized rats, the plasma testosterone level (0.34 +/- 0.07 ng.ml, mean +/- SEM) is significantly lower (P less than 0.001) than in sham operated male rats (1.7 +/- 0.26 ng.ml, mean +/- SEM). In dogs, after 90% hepatectomy, the mean plasma testosterone concentration fell to 1/10 of the plasma testosterone level measured in sham operated animals either 24 or 72 h after surgery. In hepatectomized men, plasma testosterone is markedly decreased in contrast to what is observed after duodeno-pancreatectomy performed under the same conditions of anesthesia. These results suggest that 90% hepatectomy severely alters the Leydig cell function.

Research paper thumbnail of Characterisation of a newly isolated Caco-2 clone (TC-7), as a model of transport processes and biotransformation of drugs

International Journal of Pharmaceutics, 1995

... Isabelle Caro a, Xavier Boulenc a Monique Rousset b Viviane Meunier a Martine Bourri6 a, Bern... more ... Isabelle Caro a, Xavier Boulenc a Monique Rousset b Viviane Meunier a Martine Bourri6 a, Bernand Julian a, Henri Joyeux c, Claude Roques a ... Cell culture Parental Caco-2 cells, originating from a hu-man colorectal carcinoma (Fogh et al., 1977), were obtained from Dr J. Fogh ...