Hervé Chrestin - Academia.edu (original) (raw)

Papers by Hervé Chrestin

Research paper thumbnail of Involvement of ethylene-responsive microRNAs and their targets in increased latex yield in the rubber tree in response to ethylene treatment

Plant Physiology and Biochemistry, 2014

The rubber tree is an economically important plant that produces natural rubber for various indus... more The rubber tree is an economically important plant that produces natural rubber for various industrial uses. The application of ethylene contributes to increased latex production in rubber trees; however, the molecular biology behind the effects of ethylene on latex yield remains to be elucidated. Recently, the intersection between microRNA (miRNA) regulation and phytohormone responses has been revealed. Insight into the regulation of miRNAs and their target genes should help to determine the functional importance of miRNAs as well as the role of miRNAs in signaling under ethylene stimulation in the rubber tree. In this study, hbr-miR159 and hbr-miR166 were down-regulated in bark under ethylene treatment. The ethylene also down-regulated ATHB15-like (Class III Homeodomain Leucine Zipper, HD-ZIP III) which have been extensively implicated in the regulation of primary and secondary vascular tissue pattern formation. The strong negative-regulation of ARF6/ARF8 caused by hbr-miR167 involved in an attenuation of vascular development and may gradually lead to bark dryness syndrome in the long term ethylene treatment. The negative correlation of hbr-miR172 and its target REF3 in the inner soft bark under ethylene treatment results in dramatic increases in latex yield in the ethylene-sensitive clone of the rubber tree. The overall results suggested that the differential expression of HD-ZIP III, miR167/ARF6, ARF8, and miR172/REF3 and related genes may play possible roles in the response to ethylene treatment, resulting in longer latex flow and increased latex yield.

Research paper thumbnail of Molecular characterization of new members of the Hevea brasiliensis hevein multigene family and analysis of their promoter region in rice

The cloning of hevein genes from Hevea brasiliensis was undertaken with the objective to isolate ... more The cloning of hevein genes from Hevea brasiliensis was undertaken with the objective to isolate useful promoters to drive transgene expression in genetically engineered rubber tree. Four different full length genes were cloned by library screening and a fifth, a partial gene, by adaptor-anchored PCR. Sequence alignment revealed that hevein genes, although highly conserved in their transcribed region, diverged in two groups, with major differences in their promoter region, suggesting a more rapid evolution of the upstream regulatory functions of the genes than the downstream functions of their protein products. The promoter regions from two hevein genes representative of each group were isolated and analyzed in rice. Although both were functional, only the longest promoter sequence (PHev2.1) conferred a high level of expression to the transgene in various tissues of this heterologous host. It was in addition up-regulated by mechanical wounding and fungal infection in leaves. A number of potential cis-regulatory elements were identified in silico and are discussed in view of the expression profiles observed in rice.

Research paper thumbnail of The leaf, inner bark and latex cyanide potential of Hevea brasiliensis: Evidence for involvement of cyanogenic glucosides in rubber yield

Phytochemistry, 2009

Euphorbiaceae Rubber tree Latex Leaf and bark cyanide potential Cyanide metabolism Linamarin Rubb... more Euphorbiaceae Rubber tree Latex Leaf and bark cyanide potential Cyanide metabolism Linamarin Rubber yield a b s t r a c t

Research paper thumbnail of Early effects of the mutation laurina on the functioning and size of the shoot apex in coffee tree and analysis of the plastochron phases: relationships with the dwarfism of leaves

Trees-structure and Function - TREES-STRUCT FUNCT, 2009

The current article presents the investigations into the effect of the laurina mutation on the fu... more The current article presents the investigations into the effect of the laurina mutation on the functioning and size of the shoot apical meristem (SAM) in Coffea arabica. This monolocus and Mendelian mutation is known to have pleiotropic effects on tree shape and dwarfism. A comparison between the wild type C. arabica var. Bourbon and its natural dwarf mutant C. arabica var. laurina, also called Bourbon pointu, was carried out leading to three main results: (1) the effects appeared immediately after the emergence of the buttress but did not affect the dome-shaped SAM (size and shape); (2) the effects were located at the peripheral zone and maintained subsequently within the leaf primordia; (3) the effects consisted of reduction in both the size of primordia and the height of incipient internode, consequently resulting in dwarfism of mature leaves and internodes. By contrast, the laurina mutation had no effect on the relationship between the phyllochron and the plastochron, the decuss...

Research paper thumbnail of Laurina mutation affected Coffea arabica tree size and shape mainly through internode dwarfism

Trees-structure and Function, 2009

The two varieties—Bourbon (B) and its natural mutant Bourbon pointu (BP)—of Arabica coffee (Coffe... more The two varieties—Bourbon (B) and its natural mutant Bourbon pointu (BP)—of Arabica coffee (Coffea arabica L.) differ by an epigenetic, monolocus, and recessive laurina mutation that results in pleiotropic effects, such as tree dwarfism and tree-shape modification. The objective of the study was to search for the origin of the differences in size and shape of the tree both at

Research paper thumbnail of Differential expression of microRNAs and their targets reveals a possible dual role in physiological bark disorder in rubber tree

Journal of Plant Physiology, 2014

Please cite this article as: Lertpanyasampatha M, Viboonjun U, Kongsawadworakul P, Chrestin H, Na... more Please cite this article as: Lertpanyasampatha M, Viboonjun U, Kongsawadworakul P, Chrestin H, Narangajavana J, Differential expression of microRNAs and their targets reveals a possible dual role in physiological bark disorder in rubber tree, Journal of Plant Physiology (2014), http://dx.

Research paper thumbnail of Hormonal treatment of the bark of rubber trees (Hevea brasiliensis) increases latex yield through latex dilution in relation with the differential expression of two aquaporin genes

Journal of Plant Physiology, 2011

Natural rubber is synthesized in laticifers in the inner liber of the rubber tree (Hevea brasilie... more Natural rubber is synthesized in laticifers in the inner liber of the rubber tree (Hevea brasiliensis). Upon bark tapping, the latex is expelled due to liber turgor pressure. The mature laticifers are devoid of plasmodesmata; therefore a corresponding decrease in the total latex solid content is likely to occur due to water influx inside the laticifers. Auxins and ethylene used as efficient yield stimulants in mature untapped rubber trees, but, bark treatments with abscisic acid (ABA) and salicylic acid (SA) could also induce a transient increase latex yield. We recently reported that there are three aquaporin genes, HbPIP2;1, HbTIP1;1 and HbPIP1;1, that are regulated differentially after ethylene bark treatment. HbPIP2;1 was up-regulated in both the laticifers and the inner liber tissues, whereas HbTIP1;1 was up-regulated in the latex cells, but very markedly down-regulated in the inner liber tissues. Conversely, HbPIP1;1 was down-regulated in both tissues. In the present study, HbPIP2;1 and HbTIP1;1 showed a similar expression in response to auxin, ABA and SA, as seen in ethylene stimulation, while HbPIP1;1 was slightly regulated by auxin, but neither by ABA nor SA. The analysis of the HbPIP1;1 promoter region indicated the presence of only ethylene and auxin responsive elements. In addition, the poor efficiency of this HbPIP1;1 in increasing plasmalemma water conductance was confirmed in Xenopus oocytes. Thus, an increase in latex yield in response to all of these hormones was proposed to be the major function of aquaporins, HbPIP2;1 and HbTIP1;1. This study emphasized that the circulation of water between the laticifers and their surrounding tissues that result in latex dilution, as well as the probable maintenance of the liber tissues turgor pressure, favor the prolongation of latex flow.

Research paper thumbnail of Genetic mapping of a caffeoyl-coenzyme A 3-O-methyltransferase gene in coffee trees. Impact on chlorogenic acid content

Chlorogenic acids (CGA) are involved in the bitterness of coffee due to their decomposition in ph... more Chlorogenic acids (CGA) are involved in the bitterness of coffee due to their decomposition in phenolic compounds during roasting. CGA mainly include caffeoylquinic acids (CQA), dicaffeoyl-quinic acids (diCQA) and feruloyl-quinic acids (FQA), while CQA and diCQA constitute CGA sensu stricto (CGAs.s.). In the two cultivated species Coffea canephora and Coffea arabica, CGAs.s. represents 88% and 95% of total CGA, respectively. Among all enzymes involved in CGA biosynthesis, caffeoyl-coenzyme A 3-O-methyltransferase (CCoAOMT) is not directly involved in the CGAs.s. pathway, but rather in an upstream branch leading to FQA through feruloyl-CoA. We describe how a partial cDNA corresponding to a CCoAOMT encoding gene was obtained and sequenced. Specific primers were designed and used for studying polymorphism and locating the corresponding gene on a genetic map obtained from an interspecific backcross between Coffea liberica var. Dewevrei and Coffea pseudozanguebariae. Offspring of this backcross were also evaluated for the chlorogenic acid content in their green beans. A 10% decrease was observed in backcross progenies that possess one C. pseudozanguebariae allele of the CCoAOMT gene. This suggests that CGAs.s. accumulation is dependent on the CCoAMT allele present and consequently on the activity of the encoded isoform, whereby CGA accumulation increases as the isoform activity decreases. Possible implications in coffee breeding are discussed.

Research paper thumbnail of Involvement of ethylene-responsive microRNAs and their targets in increased latex yield in the rubber tree in response to ethylene treatment

Plant Physiology and Biochemistry, 2014

The rubber tree is an economically important plant that produces natural rubber for various indus... more The rubber tree is an economically important plant that produces natural rubber for various industrial uses. The application of ethylene contributes to increased latex production in rubber trees; however, the molecular biology behind the effects of ethylene on latex yield remains to be elucidated. Recently, the intersection between microRNA (miRNA) regulation and phytohormone responses has been revealed. Insight into the regulation of miRNAs and their target genes should help to determine the functional importance of miRNAs as well as the role of miRNAs in signaling under ethylene stimulation in the rubber tree. In this study, hbr-miR159 and hbr-miR166 were down-regulated in bark under ethylene treatment. The ethylene also down-regulated ATHB15-like (Class III Homeodomain Leucine Zipper, HD-ZIP III) which have been extensively implicated in the regulation of primary and secondary vascular tissue pattern formation. The strong negative-regulation of ARF6/ARF8 caused by hbr-miR167 involved in an attenuation of vascular development and may gradually lead to bark dryness syndrome in the long term ethylene treatment. The negative correlation of hbr-miR172 and its target REF3 in the inner soft bark under ethylene treatment results in dramatic increases in latex yield in the ethylene-sensitive clone of the rubber tree. The overall results suggested that the differential expression of HD-ZIP III, miR167/ARF6, ARF8, and miR172/REF3 and related genes may play possible roles in the response to ethylene treatment, resulting in longer latex flow and increased latex yield.

Research paper thumbnail of Molecular characterization of new members of the Hevea brasiliensis hevein multigene family and analysis of their promoter region in rice

The cloning of hevein genes from Hevea brasiliensis was undertaken with the objective to isolate ... more The cloning of hevein genes from Hevea brasiliensis was undertaken with the objective to isolate useful promoters to drive transgene expression in genetically engineered rubber tree. Four different full length genes were cloned by library screening and a fifth, a partial gene, by adaptor-anchored PCR. Sequence alignment revealed that hevein genes, although highly conserved in their transcribed region, diverged in two groups, with major differences in their promoter region, suggesting a more rapid evolution of the upstream regulatory functions of the genes than the downstream functions of their protein products. The promoter regions from two hevein genes representative of each group were isolated and analyzed in rice. Although both were functional, only the longest promoter sequence (PHev2.1) conferred a high level of expression to the transgene in various tissues of this heterologous host. It was in addition up-regulated by mechanical wounding and fungal infection in leaves. A number of potential cis-regulatory elements were identified in silico and are discussed in view of the expression profiles observed in rice.

Research paper thumbnail of The leaf, inner bark and latex cyanide potential of Hevea brasiliensis: Evidence for involvement of cyanogenic glucosides in rubber yield

Phytochemistry, 2009

Euphorbiaceae Rubber tree Latex Leaf and bark cyanide potential Cyanide metabolism Linamarin Rubb... more Euphorbiaceae Rubber tree Latex Leaf and bark cyanide potential Cyanide metabolism Linamarin Rubber yield a b s t r a c t

Research paper thumbnail of Early effects of the mutation laurina on the functioning and size of the shoot apex in coffee tree and analysis of the plastochron phases: relationships with the dwarfism of leaves

Trees-structure and Function - TREES-STRUCT FUNCT, 2009

The current article presents the investigations into the effect of the laurina mutation on the fu... more The current article presents the investigations into the effect of the laurina mutation on the functioning and size of the shoot apical meristem (SAM) in Coffea arabica. This monolocus and Mendelian mutation is known to have pleiotropic effects on tree shape and dwarfism. A comparison between the wild type C. arabica var. Bourbon and its natural dwarf mutant C. arabica var. laurina, also called Bourbon pointu, was carried out leading to three main results: (1) the effects appeared immediately after the emergence of the buttress but did not affect the dome-shaped SAM (size and shape); (2) the effects were located at the peripheral zone and maintained subsequently within the leaf primordia; (3) the effects consisted of reduction in both the size of primordia and the height of incipient internode, consequently resulting in dwarfism of mature leaves and internodes. By contrast, the laurina mutation had no effect on the relationship between the phyllochron and the plastochron, the decuss...

Research paper thumbnail of Laurina mutation affected Coffea arabica tree size and shape mainly through internode dwarfism

Trees-structure and Function, 2009

The two varieties—Bourbon (B) and its natural mutant Bourbon pointu (BP)—of Arabica coffee (Coffe... more The two varieties—Bourbon (B) and its natural mutant Bourbon pointu (BP)—of Arabica coffee (Coffea arabica L.) differ by an epigenetic, monolocus, and recessive laurina mutation that results in pleiotropic effects, such as tree dwarfism and tree-shape modification. The objective of the study was to search for the origin of the differences in size and shape of the tree both at

Research paper thumbnail of Differential expression of microRNAs and their targets reveals a possible dual role in physiological bark disorder in rubber tree

Journal of Plant Physiology, 2014

Please cite this article as: Lertpanyasampatha M, Viboonjun U, Kongsawadworakul P, Chrestin H, Na... more Please cite this article as: Lertpanyasampatha M, Viboonjun U, Kongsawadworakul P, Chrestin H, Narangajavana J, Differential expression of microRNAs and their targets reveals a possible dual role in physiological bark disorder in rubber tree, Journal of Plant Physiology (2014), http://dx.

Research paper thumbnail of Hormonal treatment of the bark of rubber trees (Hevea brasiliensis) increases latex yield through latex dilution in relation with the differential expression of two aquaporin genes

Journal of Plant Physiology, 2011

Natural rubber is synthesized in laticifers in the inner liber of the rubber tree (Hevea brasilie... more Natural rubber is synthesized in laticifers in the inner liber of the rubber tree (Hevea brasiliensis). Upon bark tapping, the latex is expelled due to liber turgor pressure. The mature laticifers are devoid of plasmodesmata; therefore a corresponding decrease in the total latex solid content is likely to occur due to water influx inside the laticifers. Auxins and ethylene used as efficient yield stimulants in mature untapped rubber trees, but, bark treatments with abscisic acid (ABA) and salicylic acid (SA) could also induce a transient increase latex yield. We recently reported that there are three aquaporin genes, HbPIP2;1, HbTIP1;1 and HbPIP1;1, that are regulated differentially after ethylene bark treatment. HbPIP2;1 was up-regulated in both the laticifers and the inner liber tissues, whereas HbTIP1;1 was up-regulated in the latex cells, but very markedly down-regulated in the inner liber tissues. Conversely, HbPIP1;1 was down-regulated in both tissues. In the present study, HbPIP2;1 and HbTIP1;1 showed a similar expression in response to auxin, ABA and SA, as seen in ethylene stimulation, while HbPIP1;1 was slightly regulated by auxin, but neither by ABA nor SA. The analysis of the HbPIP1;1 promoter region indicated the presence of only ethylene and auxin responsive elements. In addition, the poor efficiency of this HbPIP1;1 in increasing plasmalemma water conductance was confirmed in Xenopus oocytes. Thus, an increase in latex yield in response to all of these hormones was proposed to be the major function of aquaporins, HbPIP2;1 and HbTIP1;1. This study emphasized that the circulation of water between the laticifers and their surrounding tissues that result in latex dilution, as well as the probable maintenance of the liber tissues turgor pressure, favor the prolongation of latex flow.

Research paper thumbnail of Genetic mapping of a caffeoyl-coenzyme A 3-O-methyltransferase gene in coffee trees. Impact on chlorogenic acid content

Chlorogenic acids (CGA) are involved in the bitterness of coffee due to their decomposition in ph... more Chlorogenic acids (CGA) are involved in the bitterness of coffee due to their decomposition in phenolic compounds during roasting. CGA mainly include caffeoylquinic acids (CQA), dicaffeoyl-quinic acids (diCQA) and feruloyl-quinic acids (FQA), while CQA and diCQA constitute CGA sensu stricto (CGAs.s.). In the two cultivated species Coffea canephora and Coffea arabica, CGAs.s. represents 88% and 95% of total CGA, respectively. Among all enzymes involved in CGA biosynthesis, caffeoyl-coenzyme A 3-O-methyltransferase (CCoAOMT) is not directly involved in the CGAs.s. pathway, but rather in an upstream branch leading to FQA through feruloyl-CoA. We describe how a partial cDNA corresponding to a CCoAOMT encoding gene was obtained and sequenced. Specific primers were designed and used for studying polymorphism and locating the corresponding gene on a genetic map obtained from an interspecific backcross between Coffea liberica var. Dewevrei and Coffea pseudozanguebariae. Offspring of this backcross were also evaluated for the chlorogenic acid content in their green beans. A 10% decrease was observed in backcross progenies that possess one C. pseudozanguebariae allele of the CCoAOMT gene. This suggests that CGAs.s. accumulation is dependent on the CCoAMT allele present and consequently on the activity of the encoded isoform, whereby CGA accumulation increases as the isoform activity decreases. Possible implications in coffee breeding are discussed.