Himisha Dixit - Academia.edu (original) (raw)

Papers by Himisha Dixit

Deciphering Diversity at er Loci for Diversification of Powdery Mildew Resistance in Pea

Agricultural biotechnology aims to scrutinize the field crops which feed amongst half of the worl... more Agricultural biotechnology aims to scrutinize the field crops which feed amongst half of the world’s population by improving their agronomic traits using various biotechnological tools. Pea- an important cash crop, rich in nutrients, but frequently infected with powdery mildew (fungal disease) that destroy the whole crop which causes the economy loss for growers. We therefore, targeted this research to find the pathogen resistant pea lines and further decipher the diversity at er locus among resistant pea lines. Screening for resistant pea lines was done with Erysiphe pisi isolates (Genebank submission: KX45 5922.1) under net house and green house conditions. Molecular studies revealed that Erysiphe resistant (er1) gene was present in 40 lines out of selected 50 pea lines and the mutational character was conferred up to 36 genotypes with 11 haplotype groups. The haplotype (gene) diversity (Hd) was found to be 0.5571±0.099 SD and the nucleotide diversity (Pi) was 0.0160±0.0042 SD Maj...

Bacopa monnieri, a traditional Indian medicinal plant with high commercial importance, is used as... more Bacopa monnieri, a traditional Indian medicinal plant with high commercial importance, is used as a neurotonic, immuno modulator, adaptogen transquilizing, memory and learning enhancing, cerebral activator, anti-ulcer, antispasmodic, anti-asthmatic ayurvedic herb. The study was undertaken in order to elaborate the tissue culture technique for Brahmi (Bacopa monnieri). In the present study nodal explants were taken for in vitro propagation. For sterilization, 0.01% HgCl2 was used as the sterilant. The explants gave best results when treated with the sterilant for 5 minutes. To induce bud-break, nodal segments were inoculated onto MS media supplemented with 1mg/l BAP. Basal MS medium without any plant growth hormone was used as control. Further multiplication was obtained on MS medium + BAP (1.0 mg/l) +IAA (0.5 mg/l). The shoots were rooted and best rooting was observed by IBA when incorporated in MS at different concentrations (0.1-0.3 mg/l) and 0.2 mg/l gave good results. After 30 days about 2.2 cm length was observed. Almost 70% of the rooted shoots survived during hardening.

World Researchers Associations, 2019

The antimicrobial activity of the extracts of the Calotropis procera, Datura metel, Cuscuta refle... more The antimicrobial activity of the extracts of the Calotropis procera, Datura metel, Cuscuta reflexa, Pongamia pinnata and Nerium oleander has been studied against the pathogenic bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aureuginosa, Klebsiella pneumoiniae, Paenibacillus and Bacilus subtilis. Significant antibacterial activity was observed with extract of ethanol, methanol and chloroform. The ethanol extract of D. metel showed maximum antibacterial activity against the all tested bacteria except the P. aureuginosa. Aqueous extract showed no measurable antibacterial activity. Inhibition was seen as concentration dependent phenomenon. Phytochemical screening revealed the presence of alkaloids, flavanoids, glycosides, steroids, tannins, phenol and saponin.

Deciphering Diversity at er Loci for Diversification of Powdery Mildew Resistance in Pea

Agricultural biotechnology aims to scrutinize the field crops which feed amongst half of the worl... more Agricultural biotechnology aims to scrutinize the field crops which feed amongst half of the world’s population by improving their agronomic traits using various biotechnological tools. Pea- an important cash crop, rich in nutrients, but frequently infected with powdery mildew (fungal disease) that destroy the whole crop which causes the economy loss for growers. We therefore, targeted this research to find the pathogen resistant pea lines and further decipher the diversity at er locus among resistant pea lines. Screening for resistant pea lines was done with Erysiphe pisi isolates (Genebank submission: KX45 5922.1) under net house and green house conditions. Molecular studies revealed that Erysiphe resistant (er1) gene was present in 40 lines out of selected 50 pea lines and the mutational character was conferred up to 36 genotypes with 11 haplotype groups. The haplotype (gene) diversity (Hd) was found to be 0.5571±0.099 SD and the nucleotide diversity (Pi) was 0.0160±0.0042 SD Maj...

Bacopa monnieri, a traditional Indian medicinal plant with high commercial importance, is used as... more Bacopa monnieri, a traditional Indian medicinal plant with high commercial importance, is used as a neurotonic, immuno modulator, adaptogen transquilizing, memory and learning enhancing, cerebral activator, anti-ulcer, antispasmodic, anti-asthmatic ayurvedic herb. The study was undertaken in order to elaborate the tissue culture technique for Brahmi (Bacopa monnieri). In the present study nodal explants were taken for in vitro propagation. For sterilization, 0.01% HgCl2 was used as the sterilant. The explants gave best results when treated with the sterilant for 5 minutes. To induce bud-break, nodal segments were inoculated onto MS media supplemented with 1mg/l BAP. Basal MS medium without any plant growth hormone was used as control. Further multiplication was obtained on MS medium + BAP (1.0 mg/l) +IAA (0.5 mg/l). The shoots were rooted and best rooting was observed by IBA when incorporated in MS at different concentrations (0.1-0.3 mg/l) and 0.2 mg/l gave good results. After 30 days about 2.2 cm length was observed. Almost 70% of the rooted shoots survived during hardening.

World Researchers Associations, 2019

The antimicrobial activity of the extracts of the Calotropis procera, Datura metel, Cuscuta refle... more The antimicrobial activity of the extracts of the Calotropis procera, Datura metel, Cuscuta reflexa, Pongamia pinnata and Nerium oleander has been studied against the pathogenic bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aureuginosa, Klebsiella pneumoiniae, Paenibacillus and Bacilus subtilis. Significant antibacterial activity was observed with extract of ethanol, methanol and chloroform. The ethanol extract of D. metel showed maximum antibacterial activity against the all tested bacteria except the P. aureuginosa. Aqueous extract showed no measurable antibacterial activity. Inhibition was seen as concentration dependent phenomenon. Phytochemical screening revealed the presence of alkaloids, flavanoids, glycosides, steroids, tannins, phenol and saponin.