Hirokazu Kouguchi - Academia.edu (original) (raw)

Papers by Hirokazu Kouguchi

Research paper thumbnail of Consumption of anthelmintic fox baits by target and non-target mammals in Japan

Russian journal of theriology, Nov 16, 2021

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Early-phase migration dynamics of Echinococcus multilocularis in two mouse strains showing different infection susceptibilities

International Journal for Parasitology, Oct 1, 2021

The early-phase migration dynamics of Echinococcus multilocularis in the intermediate hosts remai... more The early-phase migration dynamics of Echinococcus multilocularis in the intermediate hosts remain largely unknown. We compared the parasite burden in the intestine, liver and faeces of DBA/2 and C57BL/6 mouse strains using parasite-specific quantitative PCR. Our results indicated that the parasites invaded mainly from the middle segments of the small intestine and completed migration to the liver within 24 h p.i. C57BL/6 mice had lower parasite DNA burdens in the intestine and liver but higher in the faeces than DBA/2 mice, suggesting that parasite invasion of the intestine may be a critical stage regulating E. multilocularis infection in mice.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Role of C-Terminal Region of HA-33 Component of Botulinum Toxin in Hemagglutination

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Characterization of microRNAs expressed in the cystic legion of the liver of Mus musculus perorally infected with Echinococcus multilocularis Nemuro strain

Parasitology International

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Loop-mediated isothermal amplification (LAMP) for rapid and easy identification of Omphalotus japonicus

Food Chemistry: Molecular Sciences

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Usefulness of an anesthetic mixture of medetomidine, midazolam, and butorphanol in cotton rats (Sigmodon hispidus)

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Effect of the anti-parasitic compounds pyrvinium pamoate and artemisinin in enzymatic and culture assays: Data on the search for new anti-echinococcal drugs

Data in Brief, 2021

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Molecular and functional characterization of glucose transporter genes of the fox tapeworm Echinococcus multilocularis

Molecular and biochemical parasitology, 2018

Alveolar echinococcosis (AE) is a zoonotic parasitosis caused by larvae of the fox tapeworm, Echi... more Alveolar echinococcosis (AE) is a zoonotic parasitosis caused by larvae of the fox tapeworm, Echinococcus multilocularis. E. multilocularis is distributed widely in the Northern hemisphere, causing serious health problems in various animals and humans. E. multilocularis, like other cestodes, lacks a digestive tract and absorbs essential nutrients, including glucose, across the syncytial tegument on its external surface. Therefore, it is hypothesized that E. multilocularis uses glucose transporters on its surface similar to a closely-related species, Taenia solium. Based on this hypothesis, we cloned and characterized glucose transporter homologues from E. multilocularis. As a result, we obtained full-length sequences of 2 putative glucose transporter genes (EmGLUT1 and EmGLUT2) from E. multilocularis. In silico analysis predicted that these were classified in the solute carrier family 2 group. Functional expression analysis using Xenopus oocytes demonstrated clear uptake of 2-deoxy-...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Sensitivity comparison between Mini-FLOTAC and conventional techniques for the detection of Echinococcus multilocularis eggs

Parasitology International, 2021

Canines serve as the definitive host of Echinococcus multilocularis. This study evaluated the sen... more Canines serve as the definitive host of Echinococcus multilocularis. This study evaluated the sensitivity of the Mini-FLOTAC technique (MF) for the detection of E. multilocularis eggs in definitive hosts. First, we investigated the effects of heat inactivation and preservative conditions on the detection rate of eggs obtained from experimentally infected dogs. The sensitivity of MF was compared with that of eight other techniques: the centrifugal flotation with sucrose or zinc sulfate, MGL, AMS III, and a combination of MF and flotation/sedimentation techniques. Finally, we compared the sensitivity of MF and the centrifugal flotation with sucrose for the feces of E. multilocularis-infected foxes. The detection rate reached a plateau level with a specific gravity (s.g.) 1.22 for fresh eggs, but the highest rates were obtained with s.g. greater than 1.32 for heat-inactivated eggs. There was no significant difference in the detection rate among the preservative conditions. MF showed significantly higher EPG than the other techniques. Moreover, it showed higher diagnostic sensitivity for the fox feces than the centrifugal flotation technique. These results suggest that heat inactivation may alter s.g of E. multilocularis eggs and that MF with zinc sulfate (s.g. = 1.32) would be effective for detecting heat-inactivated E. multilocularis eggs.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Mass screening for Wilson's disease by automated assays at the legal 3-year-old medical health care examination

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Consumption of anthelmintic fox baits by target and non-target mammals in Japan

Russian Journal of Theriology, 2021

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Adult worm exclusion and histological data of dogs repeatedly infected with the cestode Echinococcus multilocularis

Data in Brief, 2020

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Medical Treatment of Echinococcus multilocularis and New Horizons for Drug Discovery: Characterization of Mitochondrial Complex II as a Potential Drug Target

Echinococcosis, 2017

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Molecular and functional characterization of glucose transporter genes of the fox tapeworm Echinococcus multilocularis

Molecular and Biochemical Parasitology, 2018

Alveolar echinococcosis (AE) is a zoonotic parasitosis caused by larvae of the fox tapeworm, Echi... more Alveolar echinococcosis (AE) is a zoonotic parasitosis caused by larvae of the fox tapeworm, Echinococcus multilocularis. E. multilocularis is distributed widely in the Northern hemisphere, causing serious health problems in various animals and humans. E. multilocularis, like other cestodes, lacks a digestive tract and absorbs essential nutrients, including glucose, across the syncytial tegument on its external surface. Therefore, it is hypothesized that E. multilocularis uses glucose transporters on its surface similar to a closely-related species, Taenia solium. Based on this hypothesis, we cloned and characterized glucose transporter homologues from E. multilocularis. As a result, we obtained full-length sequences of 2 putative glucose transporter genes (EmGLUT1 and EmGLUT2) from E. multilocularis. In silico analysis predicted that these were classified in the solute carrier family 2 group. Functional expression analysis using Xenopus oocytes demonstrated clear uptake of 2-deoxy-D-glucose (2-DG) by EmGLUT1, but not by EmGLUT2 in this experimental system. EmGLUT1 was shown to have relatively high glucose transport activity. Further analyses using the Xenopus oocyte system revealed that 2-DG uptake of EmGLUT1 did not depend on the presence or concentration of Na+ nor H+, respectively. Immunoblot analyses using cultured metacestode, ex vivo protoscolex, and adult worm samples demonstrated that both EmGLUTs were stably expressed during each developmental stage of the parasite. Based on the above-mentioned findings, we conclude that EmGLUT1 is a simple facilitated glucose transporter and possibly plays an important role in glucose uptake by E. multilocularis throughout its life cycle.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Analysis for genetic loci controlling protoscolex development in the Echinococcus multilocularis infection using congenic mice

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, Jan 17, 2018

The resistance/susceptibility to Echinococcus multilocularis infection in mice is genetically con... more The resistance/susceptibility to Echinococcus multilocularis infection in mice is genetically controlled. However, genetic factors responsible for these differences remain unknown. Our previous study in genetic linkage analysis has revealed that there is a significant quantitative trait locus (QTL) for the establishment of cyst (Emcys1), and a highly significant QTL for the development of protoscolex of E. multilocularis larvae (Empsc1), on mouse chromosomes 6 and 1, respectively. The current study aimed to confirm these QTLs and narrow down the critical genetic region that controls resistance/susceptibility to E. multilocularis infection by establishing congenic and subcongenic lines from C57BL/6 (B6) and DBA/2 (D2) mice. For protoscolex development phenotype, two congenic lines, B6.D2-Empsc1 and D2.B6-Empsc1 were developed, where responsible QTL, Empsc1 was introgressed from D2 into B6 background and vice versa. For cyst establishment phenotype, two congenic lines, B6.D2-Emcys1 an...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Cotton rats (Sigmodon hispidus) possess pharyngeal pouch remnants originating from different primordia

Histology and histopathology, 2018

Pharyngeal pouches in mammals develop into specific derivatives. If the differentiation of the ph... more Pharyngeal pouches in mammals develop into specific derivatives. If the differentiation of the pharyngeal pouches is anomalous, their remnants can result in cysts, sinuses, and fistulae in the differentiated organs or around the neck. In the present study, we found several pharyngeal pouch remnants, such as cystic structures in thymus and parathyroid gland and fossulae extended from the piriform fossa, in the inbred cotton rats maintained at Hokkaido Institute of Public Health (HIS/Hiph) and University of Miyazaki (HIS/Mz). In HIS/Hiph, the fossulae extended from the apex of the piriform fossa into the thyroid glands and were lined with stratified squamous and cuboidal epithelium. Calcitonin-positive C-cells were present within their epithelium in HIS/Hiph. In contrast, the fossulae of HIS/Mz ran outside the thyroid glands toward the parathyroid glands; they were lined with columnar ciliated epithelium and a few goblet cells, but had no C-cells, which was consistent with the cystic ...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Close pathological correlations between chronic kidney disease and reproductive organ-associated abnormalities in female cotton rats

Experimental biology and medicine (Maywood, N.J.), 2018

Cotton rat ( Sigmodon hispidus) is a useful experimental rodent for the study of human infectious... more Cotton rat ( Sigmodon hispidus) is a useful experimental rodent for the study of human infectious diseases. We previously clarified that cotton rats, particularly females, developed chronic kidney disease characterized by cystic lesions, inflammation, and fibrosis. The present study investigated female-associated factors for chronic kidney disease development in cotton rats. Notably, female cotton rats developed separation of the pelvic symphysis and hypertrophy in the vaginal parts of the cervix with age, which strongly associated with pyometra. The development of pyometra closely associated with the deterioration of renal dysfunction or immunological abnormalities was indicated by blood urea nitrogen and serum creatinine or spleen weight and serum albumin/globulin ratio, respectively. These parameters for renal dysfunction and immunological abnormalities were statistically correlated. These phenotypes found in the female reproductive organs were completely inhibited by ovariectomy...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of {"__content__"=>"Gene expression profiles of the small intestinal mucosa of dogs repeatedly infected with the cestode .", "i"=>{"__content__"=>"Echinococcus multilocularis"}}

Data in brief, 2018

he data set presented in this article is related to a previous research article entitled " T... more he data set presented in this article is related to a previous research article entitled " The timing of worm exclusion in dogs repeatedly infected with the cestode " (Kouguchi et al., 2016) [1]. This article describes the genes >2-fold up- or down-regulated in the first- and repeated-infection groups compared to the healthy controls group. The gene expression profiles were generated using the Agilent-021193 Canine (V2) Gene Expression Microarray (GPL15379). The raw and normalized microarray data have been deposited with the Gene Expression Omnibus (GEO) database under accession number GSE105098.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Diagnosis of canine Echinococcus multilocularis infections by copro-DNA tests: comparison of DNA extraction techniques and evaluation of diagnostic deworming

Parasitology Research, 2017

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Female cotton rats (Sigmodon hispidus) develop chronic anemia with renal inflammation and cystic changes

Histochemistry and Cell Biology, 2016

The cotton rat (Sigmodon hispidus) is a laboratory rodent that has been used for studies on human... more The cotton rat (Sigmodon hispidus) is a laboratory rodent that has been used for studies on human infectious diseases. In the present study, we observed that female cotton rats, not the male cotton rats, developed chronic anemia characterized by reduced red blood cell, hemoglobin, and hematocrit levels from 5 to 9 months of age without any changes in the mean corpuscular hemoglobin and volume levels. In peripheral blood, the reticulocyte count did not increase in response to anemia in female cotton rats, and no extramedullary hematopoiesis was observed in the liver or spleen. Further, the serum levels of urea nitrogen and creatinine increased from 5 to 9 months of age in female cotton rats compared to male cotton rats, and these increases became more prominent from 10 months of age onward, indicating chronic kidney disease. Histopathologically, female cotton rats manifested tubulointerstitial lesions characterized by the infiltration of mononuclear cells, including plasma cells and CD3(+) T-cells, as well as the dilation of calbindin-D28k(+) distal tubules from 5 to 9 months of age. The severity of these lesions progressed from 10 months of age onward, and renal fibrotic features and numerous tubular cysts appeared without any obvious glomerular lesions. A significant decrease in the erythropoietin protein levels was observed in the kidney of aged female cotton rats, and significant correlations were detected between anemia and tubulointerstitial damage. These results suggest that aged female cotton rats chronically develop renal anemia, and this rodent may serve as a novel model to elucidate its pathogenesis.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Consumption of anthelmintic fox baits by target and non-target mammals in Japan

Russian journal of theriology, Nov 16, 2021

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Early-phase migration dynamics of Echinococcus multilocularis in two mouse strains showing different infection susceptibilities

International Journal for Parasitology, Oct 1, 2021

The early-phase migration dynamics of Echinococcus multilocularis in the intermediate hosts remai... more The early-phase migration dynamics of Echinococcus multilocularis in the intermediate hosts remain largely unknown. We compared the parasite burden in the intestine, liver and faeces of DBA/2 and C57BL/6 mouse strains using parasite-specific quantitative PCR. Our results indicated that the parasites invaded mainly from the middle segments of the small intestine and completed migration to the liver within 24 h p.i. C57BL/6 mice had lower parasite DNA burdens in the intestine and liver but higher in the faeces than DBA/2 mice, suggesting that parasite invasion of the intestine may be a critical stage regulating E. multilocularis infection in mice.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Role of C-Terminal Region of HA-33 Component of Botulinum Toxin in Hemagglutination

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Characterization of microRNAs expressed in the cystic legion of the liver of Mus musculus perorally infected with Echinococcus multilocularis Nemuro strain

Parasitology International

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Loop-mediated isothermal amplification (LAMP) for rapid and easy identification of Omphalotus japonicus

Food Chemistry: Molecular Sciences

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Usefulness of an anesthetic mixture of medetomidine, midazolam, and butorphanol in cotton rats (Sigmodon hispidus)

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Effect of the anti-parasitic compounds pyrvinium pamoate and artemisinin in enzymatic and culture assays: Data on the search for new anti-echinococcal drugs

Data in Brief, 2021

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Molecular and functional characterization of glucose transporter genes of the fox tapeworm Echinococcus multilocularis

Molecular and biochemical parasitology, 2018

Alveolar echinococcosis (AE) is a zoonotic parasitosis caused by larvae of the fox tapeworm, Echi... more Alveolar echinococcosis (AE) is a zoonotic parasitosis caused by larvae of the fox tapeworm, Echinococcus multilocularis. E. multilocularis is distributed widely in the Northern hemisphere, causing serious health problems in various animals and humans. E. multilocularis, like other cestodes, lacks a digestive tract and absorbs essential nutrients, including glucose, across the syncytial tegument on its external surface. Therefore, it is hypothesized that E. multilocularis uses glucose transporters on its surface similar to a closely-related species, Taenia solium. Based on this hypothesis, we cloned and characterized glucose transporter homologues from E. multilocularis. As a result, we obtained full-length sequences of 2 putative glucose transporter genes (EmGLUT1 and EmGLUT2) from E. multilocularis. In silico analysis predicted that these were classified in the solute carrier family 2 group. Functional expression analysis using Xenopus oocytes demonstrated clear uptake of 2-deoxy-...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Sensitivity comparison between Mini-FLOTAC and conventional techniques for the detection of Echinococcus multilocularis eggs

Parasitology International, 2021

Canines serve as the definitive host of Echinococcus multilocularis. This study evaluated the sen... more Canines serve as the definitive host of Echinococcus multilocularis. This study evaluated the sensitivity of the Mini-FLOTAC technique (MF) for the detection of E. multilocularis eggs in definitive hosts. First, we investigated the effects of heat inactivation and preservative conditions on the detection rate of eggs obtained from experimentally infected dogs. The sensitivity of MF was compared with that of eight other techniques: the centrifugal flotation with sucrose or zinc sulfate, MGL, AMS III, and a combination of MF and flotation/sedimentation techniques. Finally, we compared the sensitivity of MF and the centrifugal flotation with sucrose for the feces of E. multilocularis-infected foxes. The detection rate reached a plateau level with a specific gravity (s.g.) 1.22 for fresh eggs, but the highest rates were obtained with s.g. greater than 1.32 for heat-inactivated eggs. There was no significant difference in the detection rate among the preservative conditions. MF showed significantly higher EPG than the other techniques. Moreover, it showed higher diagnostic sensitivity for the fox feces than the centrifugal flotation technique. These results suggest that heat inactivation may alter s.g of E. multilocularis eggs and that MF with zinc sulfate (s.g. = 1.32) would be effective for detecting heat-inactivated E. multilocularis eggs.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Mass screening for Wilson's disease by automated assays at the legal 3-year-old medical health care examination

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Consumption of anthelmintic fox baits by target and non-target mammals in Japan

Russian Journal of Theriology, 2021

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Adult worm exclusion and histological data of dogs repeatedly infected with the cestode Echinococcus multilocularis

Data in Brief, 2020

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Medical Treatment of Echinococcus multilocularis and New Horizons for Drug Discovery: Characterization of Mitochondrial Complex II as a Potential Drug Target

Echinococcosis, 2017

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Molecular and functional characterization of glucose transporter genes of the fox tapeworm Echinococcus multilocularis

Molecular and Biochemical Parasitology, 2018

Alveolar echinococcosis (AE) is a zoonotic parasitosis caused by larvae of the fox tapeworm, Echi... more Alveolar echinococcosis (AE) is a zoonotic parasitosis caused by larvae of the fox tapeworm, Echinococcus multilocularis. E. multilocularis is distributed widely in the Northern hemisphere, causing serious health problems in various animals and humans. E. multilocularis, like other cestodes, lacks a digestive tract and absorbs essential nutrients, including glucose, across the syncytial tegument on its external surface. Therefore, it is hypothesized that E. multilocularis uses glucose transporters on its surface similar to a closely-related species, Taenia solium. Based on this hypothesis, we cloned and characterized glucose transporter homologues from E. multilocularis. As a result, we obtained full-length sequences of 2 putative glucose transporter genes (EmGLUT1 and EmGLUT2) from E. multilocularis. In silico analysis predicted that these were classified in the solute carrier family 2 group. Functional expression analysis using Xenopus oocytes demonstrated clear uptake of 2-deoxy-D-glucose (2-DG) by EmGLUT1, but not by EmGLUT2 in this experimental system. EmGLUT1 was shown to have relatively high glucose transport activity. Further analyses using the Xenopus oocyte system revealed that 2-DG uptake of EmGLUT1 did not depend on the presence or concentration of Na+ nor H+, respectively. Immunoblot analyses using cultured metacestode, ex vivo protoscolex, and adult worm samples demonstrated that both EmGLUTs were stably expressed during each developmental stage of the parasite. Based on the above-mentioned findings, we conclude that EmGLUT1 is a simple facilitated glucose transporter and possibly plays an important role in glucose uptake by E. multilocularis throughout its life cycle.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Analysis for genetic loci controlling protoscolex development in the Echinococcus multilocularis infection using congenic mice

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, Jan 17, 2018

The resistance/susceptibility to Echinococcus multilocularis infection in mice is genetically con... more The resistance/susceptibility to Echinococcus multilocularis infection in mice is genetically controlled. However, genetic factors responsible for these differences remain unknown. Our previous study in genetic linkage analysis has revealed that there is a significant quantitative trait locus (QTL) for the establishment of cyst (Emcys1), and a highly significant QTL for the development of protoscolex of E. multilocularis larvae (Empsc1), on mouse chromosomes 6 and 1, respectively. The current study aimed to confirm these QTLs and narrow down the critical genetic region that controls resistance/susceptibility to E. multilocularis infection by establishing congenic and subcongenic lines from C57BL/6 (B6) and DBA/2 (D2) mice. For protoscolex development phenotype, two congenic lines, B6.D2-Empsc1 and D2.B6-Empsc1 were developed, where responsible QTL, Empsc1 was introgressed from D2 into B6 background and vice versa. For cyst establishment phenotype, two congenic lines, B6.D2-Emcys1 an...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Cotton rats (Sigmodon hispidus) possess pharyngeal pouch remnants originating from different primordia

Histology and histopathology, 2018

Pharyngeal pouches in mammals develop into specific derivatives. If the differentiation of the ph... more Pharyngeal pouches in mammals develop into specific derivatives. If the differentiation of the pharyngeal pouches is anomalous, their remnants can result in cysts, sinuses, and fistulae in the differentiated organs or around the neck. In the present study, we found several pharyngeal pouch remnants, such as cystic structures in thymus and parathyroid gland and fossulae extended from the piriform fossa, in the inbred cotton rats maintained at Hokkaido Institute of Public Health (HIS/Hiph) and University of Miyazaki (HIS/Mz). In HIS/Hiph, the fossulae extended from the apex of the piriform fossa into the thyroid glands and were lined with stratified squamous and cuboidal epithelium. Calcitonin-positive C-cells were present within their epithelium in HIS/Hiph. In contrast, the fossulae of HIS/Mz ran outside the thyroid glands toward the parathyroid glands; they were lined with columnar ciliated epithelium and a few goblet cells, but had no C-cells, which was consistent with the cystic ...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Close pathological correlations between chronic kidney disease and reproductive organ-associated abnormalities in female cotton rats

Experimental biology and medicine (Maywood, N.J.), 2018

Cotton rat ( Sigmodon hispidus) is a useful experimental rodent for the study of human infectious... more Cotton rat ( Sigmodon hispidus) is a useful experimental rodent for the study of human infectious diseases. We previously clarified that cotton rats, particularly females, developed chronic kidney disease characterized by cystic lesions, inflammation, and fibrosis. The present study investigated female-associated factors for chronic kidney disease development in cotton rats. Notably, female cotton rats developed separation of the pelvic symphysis and hypertrophy in the vaginal parts of the cervix with age, which strongly associated with pyometra. The development of pyometra closely associated with the deterioration of renal dysfunction or immunological abnormalities was indicated by blood urea nitrogen and serum creatinine or spleen weight and serum albumin/globulin ratio, respectively. These parameters for renal dysfunction and immunological abnormalities were statistically correlated. These phenotypes found in the female reproductive organs were completely inhibited by ovariectomy...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of {"__content__"=>"Gene expression profiles of the small intestinal mucosa of dogs repeatedly infected with the cestode .", "i"=>{"__content__"=>"Echinococcus multilocularis"}}

Data in brief, 2018

he data set presented in this article is related to a previous research article entitled " T... more he data set presented in this article is related to a previous research article entitled " The timing of worm exclusion in dogs repeatedly infected with the cestode " (Kouguchi et al., 2016) [1]. This article describes the genes >2-fold up- or down-regulated in the first- and repeated-infection groups compared to the healthy controls group. The gene expression profiles were generated using the Agilent-021193 Canine (V2) Gene Expression Microarray (GPL15379). The raw and normalized microarray data have been deposited with the Gene Expression Omnibus (GEO) database under accession number GSE105098.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Diagnosis of canine Echinococcus multilocularis infections by copro-DNA tests: comparison of DNA extraction techniques and evaluation of diagnostic deworming

Parasitology Research, 2017

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Female cotton rats (Sigmodon hispidus) develop chronic anemia with renal inflammation and cystic changes

Histochemistry and Cell Biology, 2016

The cotton rat (Sigmodon hispidus) is a laboratory rodent that has been used for studies on human... more The cotton rat (Sigmodon hispidus) is a laboratory rodent that has been used for studies on human infectious diseases. In the present study, we observed that female cotton rats, not the male cotton rats, developed chronic anemia characterized by reduced red blood cell, hemoglobin, and hematocrit levels from 5 to 9 months of age without any changes in the mean corpuscular hemoglobin and volume levels. In peripheral blood, the reticulocyte count did not increase in response to anemia in female cotton rats, and no extramedullary hematopoiesis was observed in the liver or spleen. Further, the serum levels of urea nitrogen and creatinine increased from 5 to 9 months of age in female cotton rats compared to male cotton rats, and these increases became more prominent from 10 months of age onward, indicating chronic kidney disease. Histopathologically, female cotton rats manifested tubulointerstitial lesions characterized by the infiltration of mononuclear cells, including plasma cells and CD3(+) T-cells, as well as the dilation of calbindin-D28k(+) distal tubules from 5 to 9 months of age. The severity of these lesions progressed from 10 months of age onward, and renal fibrotic features and numerous tubular cysts appeared without any obvious glomerular lesions. A significant decrease in the erythropoietin protein levels was observed in the kidney of aged female cotton rats, and significant correlations were detected between anemia and tubulointerstitial damage. These results suggest that aged female cotton rats chronically develop renal anemia, and this rodent may serve as a novel model to elucidate its pathogenesis.

Bookmarks Related papers MentionsView impact