K. Houghton - Academia.edu (original) (raw)
Papers by K. Houghton
International journal of systematic and evolutionary microbiology, Jan 14, 2015
An aerobic, thermophilic and cellulolytic bacterium, designated strain WKT50.2T, was isolated fro... more An aerobic, thermophilic and cellulolytic bacterium, designated strain WKT50.2T, was isolated from geothermal soil at Waikite, New Zealand. Strain WKT50.2T grew at 53-76 °C, and at pH 5.9-8.2. The DNA G+C content was 58.4 mol%. The major fatty acids were 12-methyl C18:0 and C18:0. Polar lipids were all linked to long chain 1,2-diols, and comprised of 2-acylalkyldiol-1-O-phosphoinositol (diolPI), 2 acylalkyldiol-1-O-phospho-acylmannoside (diolP-acylMan), 2-acylalkyldiol-1-O-phosphoinositol acylmannoside (diolPI-acylMan) and 2-acylalkyldiol-1-O-phosphoinositol mannoside (diolPI-Man). Strain WKT50.2T was utilised a range of cellulosic substrates, alcohols and organic acids for growth, but was unable to utilise monosaccharides. Robust growth of WKT50.2T was observed on protein derivatives. WKT50.2T was sensitive to ampicillin, chloramphenicol, kanamycin, neomycin, polymyxin B, streptomycin and vancomycin. Metronidazole, lasalocid A and trimethoprim stimulated growth. Phylogenetic analys...
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2014
A strictly aerobic, thermophilic, moderately acidophilic, non-spore-forming bacterium, strain P37... more A strictly aerobic, thermophilic, moderately acidophilic, non-spore-forming bacterium, strain P373 T , was isolated from geothermally heated soil at Waikite, New Zealand. Cells were filamentous rods, 0.2-0.4 mm in diameter and grew in chains up to 80 mm in length. On the basis of 16S rRNA gene sequence similarity, strain P373 T was shown to belong to the family Chitinophagaceae (class Sphingobacteriia) of the phylum Bacteroidetes, with the most closely related cultivated strain, Chitinophaga pinensis UQM 2034 T , having 87.6 % sequence similarity. Cells stained Gram-negative, and were catalase-and oxidase-positive. The major fatty acids were i-15 : 0 (10.8 %), i-17 : 0 (24.5 %) and i-17 : 0 3-OH (35.2 %). Primary lipids were phosphatidylethanolamine, two unidentified aminolipids and three other unidentified polar lipids. The presence of sulfonolipids (N-acyl-capnines) was observed in the total lipid extract by mass spectrometry. The G+C content of the genomic DNA was 47.3 mol% and the primary respiratory quinone was MK-7. Strain P373 T grew at 35-63 6C with an optimum temperature of 60 6C, and at pH 5.5-8.7 with an optimum growth pH of 7.3-7.4. NaCl tolerance was up to 5 % (w/v) with an optimum of 0.1-0.25 % (w/v). Cell colonies were non-translucent and pigmented vivid yelloworange. Cells displayed an oxidative chemoheterotrophic metabolism. The distinct phylogenetic position and the phenotypic characteristics separate strain P373 T from all other members of the phylum Bacteroidetes and indicate that it represents a novel species in a new genus, for which the name Thermoflavifilum aggregans gen. nov., sp. nov. is proposed. The type strain of the type species is P373 T (5ICMP 20041 T 5DSM 27268 T ).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2011
International journal of systematic and evolutionary microbiology, Jan 14, 2015
An aerobic, thermophilic and cellulolytic bacterium, designated strain WKT50.2T, was isolated fro... more An aerobic, thermophilic and cellulolytic bacterium, designated strain WKT50.2T, was isolated from geothermal soil at Waikite, New Zealand. Strain WKT50.2T grew at 53-76 °C, and at pH 5.9-8.2. The DNA G+C content was 58.4 mol%. The major fatty acids were 12-methyl C18:0 and C18:0. Polar lipids were all linked to long chain 1,2-diols, and comprised of 2-acylalkyldiol-1-O-phosphoinositol (diolPI), 2 acylalkyldiol-1-O-phospho-acylmannoside (diolP-acylMan), 2-acylalkyldiol-1-O-phosphoinositol acylmannoside (diolPI-acylMan) and 2-acylalkyldiol-1-O-phosphoinositol mannoside (diolPI-Man). Strain WKT50.2T was utilised a range of cellulosic substrates, alcohols and organic acids for growth, but was unable to utilise monosaccharides. Robust growth of WKT50.2T was observed on protein derivatives. WKT50.2T was sensitive to ampicillin, chloramphenicol, kanamycin, neomycin, polymyxin B, streptomycin and vancomycin. Metronidazole, lasalocid A and trimethoprim stimulated growth. Phylogenetic analys...
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2014
A strictly aerobic, thermophilic, moderately acidophilic, non-spore-forming bacterium, strain P37... more A strictly aerobic, thermophilic, moderately acidophilic, non-spore-forming bacterium, strain P373 T , was isolated from geothermally heated soil at Waikite, New Zealand. Cells were filamentous rods, 0.2-0.4 mm in diameter and grew in chains up to 80 mm in length. On the basis of 16S rRNA gene sequence similarity, strain P373 T was shown to belong to the family Chitinophagaceae (class Sphingobacteriia) of the phylum Bacteroidetes, with the most closely related cultivated strain, Chitinophaga pinensis UQM 2034 T , having 87.6 % sequence similarity. Cells stained Gram-negative, and were catalase-and oxidase-positive. The major fatty acids were i-15 : 0 (10.8 %), i-17 : 0 (24.5 %) and i-17 : 0 3-OH (35.2 %). Primary lipids were phosphatidylethanolamine, two unidentified aminolipids and three other unidentified polar lipids. The presence of sulfonolipids (N-acyl-capnines) was observed in the total lipid extract by mass spectrometry. The G+C content of the genomic DNA was 47.3 mol% and the primary respiratory quinone was MK-7. Strain P373 T grew at 35-63 6C with an optimum temperature of 60 6C, and at pH 5.5-8.7 with an optimum growth pH of 7.3-7.4. NaCl tolerance was up to 5 % (w/v) with an optimum of 0.1-0.25 % (w/v). Cell colonies were non-translucent and pigmented vivid yelloworange. Cells displayed an oxidative chemoheterotrophic metabolism. The distinct phylogenetic position and the phenotypic characteristics separate strain P373 T from all other members of the phylum Bacteroidetes and indicate that it represents a novel species in a new genus, for which the name Thermoflavifilum aggregans gen. nov., sp. nov. is proposed. The type strain of the type species is P373 T (5ICMP 20041 T 5DSM 27268 T ).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2011