Huee Lee - Academia.edu (original) (raw)
Uploads
Papers by Huee Lee
Oncotarget
Metastatic cancer cells express Membrane Type 1-Matrix Metalloproteinase (MT1-MMP) to degrade the... more Metastatic cancer cells express Membrane Type 1-Matrix Metalloproteinase (MT1-MMP) to degrade the extracellular matrix in order to facilitate migration and proliferation. Tissue Inhibitor of Metalloproteinase (TIMP)-2 is the endogenous inhibitor of the MMP. Here, we describe a novel and highly effective fusion strategy to enhance the delivery of TIMP-2 to MT1-MMP. We can reveal that TIMP-2 fused to the haemopexin +/-transmembrane domains of MT1-MMP (two chimeras named T2 PEX+TM and T2 PEX) are able to interact with MT1-MMP on the cell surface as well as intracellularly. In the case of T2 PEX+TM , there is even a clear sign of MT1-MMP:T2 PEX+TM aggregation by the side of the nucleus to form aggresomes. In vitro, T2 PEX+TM and T2 PEX suppress the gelatinolytic and invasive abilities of cervical carcinoma (HeLa) and HT1080 fibrosarcoma cancer cells significantly better than wild type TIMP-2. In mouse xenograft, we further demonstrate that T2 PEX diminishes cervical carcinoma growth by 85% relative to the control. Collectively, our findings indicate the effectiveness of the fusion strategy as a potential targeted approach in cancer inhibition.
Oncotarget
Metastatic cancer cells express Membrane Type 1-Matrix Metalloproteinase (MT1-MMP) to degrade the... more Metastatic cancer cells express Membrane Type 1-Matrix Metalloproteinase (MT1-MMP) to degrade the extracellular matrix in order to facilitate migration and proliferation. Tissue Inhibitor of Metalloproteinase (TIMP)-2 is the endogenous inhibitor of the MMP. Here, we describe a novel and highly effective fusion strategy to enhance the delivery of TIMP-2 to MT1-MMP. We can reveal that TIMP-2 fused to the haemopexin +/-transmembrane domains of MT1-MMP (two chimeras named T2 PEX+TM and T2 PEX) are able to interact with MT1-MMP on the cell surface as well as intracellularly. In the case of T2 PEX+TM , there is even a clear sign of MT1-MMP:T2 PEX+TM aggregation by the side of the nucleus to form aggresomes. In vitro, T2 PEX+TM and T2 PEX suppress the gelatinolytic and invasive abilities of cervical carcinoma (HeLa) and HT1080 fibrosarcoma cancer cells significantly better than wild type TIMP-2. In mouse xenograft, we further demonstrate that T2 PEX diminishes cervical carcinoma growth by 85% relative to the control. Collectively, our findings indicate the effectiveness of the fusion strategy as a potential targeted approach in cancer inhibition.