Huseyin Guducuoglu - Academia.edu (original) (raw)

Papers by Huseyin Guducuoglu

Electronic Journal of General Medicine, 2022

Automatic identification and antimicrobial susceptibility systems are frequently used to identify... more Automatic identification and antimicrobial susceptibility systems are frequently used to identify clinical isolates in hospitalized patients, but mistakes in these systems can lead to potentially devastating treatment failures for patients. Therefore, the ''Centers for Disease Control and Prevention (CDC)'' recommends confirming all Carbapenem-resistant and low-susceptibility isolates with a different method. The aim of this study is to compare the Carbapenem susceptibility results of isolates reported as Carbapenem-resistant Enterobacteriaceae according to the BD Phoenix 100 automated system with the E-test method. Materials and Methods: The study included 70 strains of Carbapenem-resistant Enterobacteriaceae members which were isolated and grown from several types of clinical samples in the Medical Microbiology Laboratory. Conventional methods (Gram stain, negative oxidase test) and the BD Phoenix 100 automated system were used to identify the isolates. The susceptibility of all strains to imipenem, ertapenem and meropenem was investigated by E-test method. Automated system results and E-test results were compared. Results: The frequency distribution of all isolated bacterial strains comprised K. pneumoniae in 56 (80%) of the samples included in the study. The automated system test results were correlated with the results of the E-test at a rate of 96.1 % for the imipenem-resistant strains, 84.3% for the meropenem-resistant strains, 84.1% for the ertapenem-resistant strains Conclusions: Automated systems are frequently used in microbiology laboratories to identify isolates. However, automated systems can show a high error rate against some antimicrobials. For this reason, comparing the results of automated system test results with tests such as E-test is very important to prevent both treatment failures and inappropriate antibiotic use that may occur on a patient basis.

Copyright © 2015 Mahmut Alpayci et al. This is an open access article distributed under the Creat... more Copyright © 2015 Mahmut Alpayci et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Citrullinated proteins have been suggested to play a critical role in the pathogenesis of multiple sclerosis (MS). Anticyclic citrullinated peptide (anti-CCP) antibody is used in the early diagnosis of rheumatoid arthritis (RA).The objective of this study was to investigate the presence of anti-CCP antibody in patients with MS compared to RA patients and healthy controls. Fifty patients with MS (38 females, 12 males; mean age 36.72 ± 8.82 years), 52 patients with RA (40 females, 12 males; mean age 40.87 ± 10.17 years), and 50 healthy controls (32 females, 18 males; mean age 38.22 ± 11.59 years) were included in this study. The levels of serum anti-CCP antibody were measured using an enzyme-linked immunosorbent assay (ELISA). The resu...

Sakarya Medical Journal, 2011

Amaç: Karbapenemlerin en yeni üyesi olan ve ülkemizde son dönemde kullanıma giren doripenemin noz... more Amaç: Karbapenemlerin en yeni üyesi olan ve ülkemizde son dönemde kullanıma giren doripenemin nozokomiyal Pseudomonas aeruginosa (P.aeruginosa) izolatlarında in-vitro etkinliğinin araştırılması amaçlanmıştır. Gereç ve Yöntem: Bu amaçla 2008-2010 yılları arasında çeşitli klinik örneklerden izole edilen ve nozokomiyal infeksiyon etkeni olduğu belirlenen 86 P.aeruginosa izolatı çalışmaya alındı. P.aeruginosa izolatlarının doripenem duyarlılıkları E-test yöntemi (AB bioMérieux, Fransa) ile EUCAST'ın belirlediği minimal inhibitör konsantrasyon (MİK) değerleri (S ≤ 1 µg/ml, R > 4 µg/ml) esas alınarak saptandı, diğer antibiyotik duyarlılıkları ise BD Phoenix otomatize mikrobiyoloji sistemi (Becton Dickinson, A.B.D.) ile belirlendi. Bulgular: Çalışmaya dahil edilen P.aeruginosa suşları 64 trakea, 9 kan, 6 yara, 3 idrar, 2 kulak ile birer parasentez ve plevral mayi örneğinden izole edildi. E-test yöntemiyle 86 izolattan 34'ü (%40) doripeneme duyarlı, 52'si (%60) ise dirençli bulundu. Antibiyotik duyarlılık testi sonucunda amikasine %14, siprofloksasine %36, seftazidime %43, levofloksasine %45, meropeneme %51, piperasilin-tazobaktama %55, piperasiline %56, imipeneme %60, gentamisine %65 ve sefepim ile aztreonama %69 oranında direnç saptandı. Sonuçlar: Nozokomiyal P.aeruginosa suşlarında yüksek oranda doripenem direncinin saptandığı çalışmamızda imipenem ve diğer antibiyotiklere karşı da yüksek oranlarda direnç tespit edilmiştir. Doripenem direncinin yüksek olmasına karşın nispeten daha düşük MİK düzeylerine sahip olması ve uzun süreli infüzyon ile daha yüksek serum konsantrasyonları elde edilmesi, P.aeruginosa ile gelişen infeksiyonların tedavisinde doripenemin iyi bir seçenek olabileceğini göstermektedir. Bununla beraber çoklu ilaç direncine sahip suşların yayılmasını engellemek için infeksiyon kontrol önlemlerine sıkı bir şekilde uyulmalıdır.

International Journal of Medical Sciences, 2013

Brucellosis is a worldwide zoonotic disease and still constitutes a major public health problem. ... more Brucellosis is a worldwide zoonotic disease and still constitutes a major public health problem. In this study, we aimed to identify biovars of Brucella strains isolated from clinical specimens taken from brucellosis patients from the Eastern Anatolia region as well determine the susceptibility of these isolates to tigecycline and azithromycin, drugs that may serve as alternatives to the conventional drugs used in the therapy. Materials and methods: Seventy-five Brucella spp. isolates were included in the study. All strains were identified by both conventional and molecular methods. Brucella Multiplex PCR kit (FC-Biotech, Code: 0301, Turkey) and B. melitensis biovar typing PCR kit (FC-Biotech, Code: 0302, Turkey) were used for molecular typing. Antimicrobial susceptibilities of all strains were determined by E-tests. Results: By conventional biotyping, 73 strains were identified as B. melitensis biovar 3 and two strains as B. abortus biovar 3. Molecular typing results were compatible with conventional methods. The MIC 50 and MIC 90 values of doxycycline were 0.047 and 0.094; tigecycline 0.094 and 0.125; trimethoprim/sulfamethoxazole 0.064 and 0.19; ciprofloxacin 0.19 for both; streptomycin 0.75 and 1; rifampin 1 and 2 and azithromycin 4 and 8. According to the MIC values, doxycycline was found to be the most effective antibiotic, followed by tigecycline, trimethoprim-sulfamethoxazole and ciprofloxacin. Conclusion: Currently recommended antibiotics for the treatment of brucellosis such as doxycycline, rifampin, streptomycin, trimethoprim-sulfamethoxazole and ciprofloxacin were found to be still effective. While our results showed that tigecycline can be used an alternative agent in the treatment of brucellosis, azithromycin has not been confirmed as an appropriate agent for the treatment.

Clinical and Vaccine Immunology, 2013

ABSTRACTStreptococcus pneumoniaeis the most common etiological cause of complicated pneumonia, in... more ABSTRACTStreptococcus pneumoniaeis the most common etiological cause of complicated pneumonia, including empyema. In this study, we investigated the serotypes ofS. pneumoniaethat cause empyema in children. One hundred fifty-six children who were diagnosed with pneumonia complicated with empyema in 13 hospitals in seven geographic regions of Turkey between 2010 and 2012 were included in this study. Pleural fluid samples were collected by thoracentesis and tested for 14 serotypes/serogroups using a Bio-Plex multiplex antigen detection assay. The serotypes ofS. pneumoniaewere specified in 33 of 156 samples. The mean age ± the standard deviation of the 33 patients was 6.17 ± 3.54 years (range, 0.6 to 15 years). All of the children were unvaccinated according to the vaccination reports. Eighteen of the children were male, and 15 were female. The serotypes of the non-7-valent pneumococcal conjugated vaccine (non-PCV-7), serotype 1, serotype 5, and serotype 3, were detected in eight (14.5%...

vantipdergisi.yyu.edu.tr

... Mantar Hastalıkları Açısından Değerlendirilmesi Hüseyin Güdücüoğlu*, Necmettin Akdeniz**, Ham... more ... Mantar Hastalıkları Açısından Değerlendirilmesi Hüseyin Güdücüoğlu*, Necmettin Akdeniz**, Hamza Bozkurt*, Kumru Aygül*, Hicran İzci*, Mustafa Berktaş* ... 11. Metin A, Berktaş M, Güzeloğlu M, Delice İ, Subaşı Ş. Van Yöresinde Görülen Yüzeyel Mantar Hastalıkları. ...

Objective. Recently, community and hospital-acquired infections with Staphylococcus aureus have i... more Objective. Recently, community and hospital-acquired infections with Staphylococcus aureus have increased and raised antibiotic resistant isolates. In this study, we aimed to evaluate the antibiotic resistance profile of S. aureus isolates over several years in various clinical specimens from our hospital. Materials and Methods. S. aureus strains from 2009 to 2014 were isolated from various clinical samples at Yuzuncu Yil University, Dursun Odabas Medical Center, Microbiology Laboratory, and their antibiotic susceptibility test results were retrospectively investigated. The isolates were identified by conventional methods, and antibiotic susceptibility tests were performed by the Phoenix (Becton Dickinson, USA) automated system method according to Clinical and Laboratory Standards Institute (CLSI) standards. Results. A total of 1,116 S. aureus isolates were produced and methicillinresistant S. aureus (MRSA) to 21% of all S. aureus isolates between 2009 and 2014. According to the res...

Citrullinated proteins have been suggested to play a critical role in the pathogenesis of multipl... more Citrullinated proteins have been suggested to play a critical role in the pathogenesis of multiple sclerosis (MS). Anticyclic citrullinated peptide (anti-CCP) antibody is used in the early diagnosis of rheumatoid arthritis (RA). The objective of this study was to investigate the presence of anti-CCP antibody in patients with MS compared to RA patients and healthy controls. Fifty patients with MS (38 females, 12 males; mean age 36.72 ± 8.82 years), 52 patients with RA (40 females, 12 males; mean age 40.87 ± 10.17 years), and 50 healthy controls (32 females, 18 males; mean age 38.22 ± 11.59 years) were included in this study. The levels of serum anti-CCP antibody were measured using an enzyme-linked immunosorbent assay (ELISA). The results of the study showed that anti-CCP antibody levels were significantly higher in RA patients versus MS or healthy controls (< 0.001). Moreover, anti-CCP antibody was positive in 43 (83%) patients with RA, while it was negative in all MS patients as well as in all healthy controls. Also, no significant correlation was found between the anti-CCP levels and EDSS scores (= −0.250). In conclusion, the results of this study did not support a positive association between serum anti-CCP antibody and MS.

Medical Science and Discovery (http://www.medscidiscovery.com) is an international open access, p... more Medical Science and Discovery (http://www.medscidiscovery.com) is an international open access, peer-reviewed scientific research journal that provides rapid publication of articles in all disciplines of human health, clinical and basic The policy of top priority of MSD is to put forward and highlight medical innovations and inspiring patents. MSD offers an exceptionally fast publication schedule including prompt peer-review by the experts in the field and immediate publication upon acceptance. The editorial board aims at reviewing the submitted articles as fast as possible and promptly including them in the forthcoming issues. This journal is published under ethical publishing policy of international scientific Bioethics and publication rules. MSD supports the Open Access Initiative. Abstracts and full texts (HTML and PDF format) of all articles published by MSD are freely accessible to everyone immediately upon publication. All the financial and ethical rights of the journal are r...

Medical Science and Discovery

Objective: Silymarin is a natural extract from milk thistle (Silybum marianum), a natural herb th... more Objective: Silymarin is a natural extract from milk thistle (Silybum marianum), a natural herb that contains flavonoids. Silymarin also has anti-inflammatory properties and lipid peroxidation effects on human hepatocytes. It has also been used for the treatment of acute alpha-amanitin poisoning and chronic hepatitis C infection. Chronic Hepatitis D virus (HDV) infection is a severe health problem leading to fibrosis and hepatocellular carcinoma. Patients with chronic HDV infection can be treated with Peg-IFN with lower treatment success. Most patients with chronic HDV are unable or unwilling to use interferon (IFN)-based treatment due to liver cirrhosis. Our objective was to establish the long-term clinical outcomes with silymarin for interferon-experienced chronic HDV patients. Materials and Methods: We studied ten patients from one centre with interferon who experienced chronic HDV, of which 8 had cirrhosis, and 2 had chronic hepatitis who received HDV treatment with silymarin 6...

Advances in Dermatology and Allergology

Introduction: Genital warts are benign epithelial tumours caused by human papilloma viruses (HPV)... more Introduction: Genital warts are benign epithelial tumours caused by human papilloma viruses (HPV), and are sexually transmitted. Genotyping of genital HPV bears great clinical significance in terms of treatment planning, follow-up, and prevention strategies. Aim: To evaluate the distribution of high-risk HPV infection types in patients diagnosed with anogenital warts. Material and methods: A total of 66 patients with anogenital warts were enrolled. Punch biopsy samples were obtained from the lesions of each patient. After nucleic acid purification and DNA extraction, the presence of HPV DNA was ascertained using the PCR method, followed by HPV DNA genotyping. The relationship between HPV type distribution and age, gender, clinical location, and number of sexual partners was investigated. Results: Genotyping was performed and HPV genome was detected in 50 tissue samples (75.8%). Low-risk genotypes predominated with a prevalence of 62.1% (42/66). The most prevalent genotypes were HPV-6 (47%), and HPV-11 (13.6%). Other types detected included HPV-18 and HPV-3. Conclusions: Genotyping of HPV provides significant clinical information regarding this family of viruses that play a role in the aetiology of a variety of genital cancers, as some of these malignancies are now considered preventable due to recent development of vaccines. We believe that our results may provide guidance on future vaccination programs in our country.

Klimik Dergisi/Klimik Journal

Objective: Laboratory diagnosis of Brucella canis infections cannot be made by classical serologi... more Objective: Laboratory diagnosis of Brucella canis infections cannot be made by classical serological methods as readily as infections of other species of Brucella pathogenic for humans. Therefore, the information about B. canis infections in Turkey is limited. In this study, we aimed to detect presence of B. canis coinfections in humans who were diagnosed as brucellosis. Methods: Study has been designed as retrospective cross-sectional. A total of 566 serum samples collected from patients who had confirmed brucellosis and were living in cities of the Eastern and Southeastern Anatolia regions of Turkey were tested with rapid slide agglutination test (RSAT), 2-mercaptoethanol RSAT (2ME-RSAT) and microplate agglutination test (MAT) using B. canis M-, a less mucoid variant, as the antigen. Results: Out of the samples tested, 142 (25%) and 49 (8.7%) were positive by RSAT and 2ME-RSAT, respectively, and this difference was significant (p≤0.05). In total, 55/566 (9.7%) samples were MAT-positive. Differences between 2ME-RSAT and MAT were not statistically significant (p>0.05). Conclusions: Our B. canis coinfection prevalence data demonstrates that serological testing for B. canis should be performed in Turkey. It was also concluded that there are some advantages for using MAT, and this technique may be preferable over 2ME-RSAT.

Central European journal of public health, Jun 1, 2018

Hepatitis C virus (HCV) infection is a major public health problem and affects large populations ... more Hepatitis C virus (HCV) infection is a major public health problem and affects large populations all over the world. Serum anti-HCV level is a valuable marker to determine HCV infection. Anti-HCV testing has been recommended for high-risk population. The Center for Disease Control (CDC) and Prevention in the United States proposed a new high-risk population group - adults born between 1945-1965. Under this perspective, we designed a multicentre retrospective study to determine the seropositivity of anti-HCV among adults born between 1945 and 1965 and adults born after 1965 in Turkey. With the data collected, we aimed to determine whether there was a need for anti-HCV testing especially in people born between 1945 and 1965. We requested data from ten different medical centres in ten different provinces. Each medical centre collected the anti-HCV test results of adult patients for five-year period between 2009 and 2014 from hospital records. A total of 974,449 anti-HCV test results we...

Turk pediatri arsivi, 2017

Microbial drug resistance (Larchmont, N.Y.), Jan 21, 2017

Colistin resistance causes substantial problems in the treatment of serious infections with carba... more Colistin resistance causes substantial problems in the treatment of serious infections with carbapenem-resistant (CR) gram-negative bacteria. In this study, we report a fatal hospital outbreak from the spread of a pandrug-resistant Klebsiella pneumoniae clone. An outbreak investigation was conducted after consecutive isolation of nine CR-K. pneumoniae (CR-Kp) strains from eight patients in two intensive care units of a university hospital within 2 weeks. Carbapenem and colistin resistance genes were investigated with PCR, clonal relationships of isolates were studied with pulse-field gel electrophoresis, and multilocus sequence types were determined. The outcomes of the affected patients were analyzed. Genotyping showed a predominant CR-Kp clone consisting of seven strains from six patients. These strains were in ST11 type, an international high-risk clone. They were resistant to all antimicrobials, including colistin, and positive for NDM-1 and OXA-48 carbapenemases, but negative f...

Archives of Medical Science

Introduction: Hepatitis D virus (HDV) infection is a serious health problem leading to cirrhosis ... more Introduction: Hepatitis D virus (HDV) infection is a serious health problem leading to cirrhosis and hepatocellular carcinoma (HCC). Despite evidence that zoonotic infections are associated with end-stage liver disease, brucellosis in patients with delta hepatitis related to liver disease has not been well characterized. So, we examined this relationship using recent hospital-based data. Material and methods: We analyzed data from 96 delta hepatitis patients (mean age: 52.5 ±12.8 years; 50 male; 52 cirrhotics) and 117 (mean age: 50.4 ±7 years; 60 male) control subjects who were selected from patients with splenomegaly. The Brucella Wright test in connection with blood culture was used to detect active Brucella infection. Demographic features, laboratory data, results of ultrasonographic examination of the abdomen and Wright agglutination titers were compared between groups. Results: There were 9 (9%) patients with active brucellosis in delta hepatitis patients. Compared to the control group, there was a statistically significant difference between groups in terms of having active brucellosis (9 vs. 2 patients; p < 0.001). Higher MELD scores were also associated with active Brucella infection (p < 0.005). Conclusions: Patients with chronic hepatitis D related cirrhosis (CHD-C) were at risk of developing brucellosis requiring hospitalization. Higher Wright titers among patients with more advanced liver disease may reflect a unique phenomenon that requires further investigation to determine underlying causative factors.

Türk Pediatri Arşivi

Aim: Hepatitis E virus is an etiological agent of hepatitis which is transmitted enterically and ... more Aim: Hepatitis E virus is an etiological agent of hepatitis which is transmitted enterically and may lead to water-born outbreaks. Although it is mainly transmitted by the fecal-oral route, it is estimated that many cases are associated with zoonotic transmission in developing countries. In this study, we aimed to investigate the seroprevalence of hepatitis E in the childhood age group in the province of Van and to demonstrate the relationship between seroprevalence and demographic properties, residential house/region, water supply used at home, dealing with livestock and history of surgery. Material and Methods: In this study, hepatitis E virus IgG antibody was studied by ELISA method in children aged between 2 months and 18 years between June 2014 and September 2014 in the province of Van. Results: A total of 408 children and adolescents were enrolled in the study. Hepatitis E IgG was found to be positive in 4.2% of the subjects. 179 (43.8%) of the subjects were female and 229 (56.2%) were male. The mean age was 123 months±56.6 months (minimum 2 months, maximum 214 months). When the seropositivity rates were compared by age groups and gender, no difference was found. No correlation was found between hepatitis E seropositivity and the variables of residence, dealing with livestock and water resources. No correlation was found between anti-hepatits E virus seropositivity and parental education level, number of cohabitants and history of surgery. Conclusion: In our study, hepatitis E virus seropositivity was found to be lower compared to the mean seropositivity in Turkey. Hepatitis E infection does not constitute a serious problem in children in the province of Van in accordance with the results reported from different parts of our country. Livestock dealing and usage of well water are not considered risk factors for Hepatitis E infection.

Human vaccines & immunotherapeutics, Jan 3, 2017

Invasive pneumococcal infections among children are a serious public health problem in many count... more Invasive pneumococcal infections among children are a serious public health problem in many countries, including Turkey. Pneumococcal conjugate vaccine has been included in Turkey's National Immunization Programme since 2009. We report the first two pediatric cases of invasive pneumococcal infection due to non-vaccine serotype 15A after pneumococcal conjugate vaccine implementation in Turkey. It is essential to monitor the countries' own local seroepidemiologic data for detecting selective pressure of non-vaccine serotypes of S. pneumonia.

Medical Science and Discovery, 2016

Conventional bacteriological microscopy and culture are commonly used for the diagnosis of tuberc... more Conventional bacteriological microscopy and culture are commonly used for the diagnosis of tuberculosis, particularly in developing countries. However, their limited sensitivity, specificity, and delayed results make this provision inadequate.

Wiener klinische Wochenschrift, 2016

Squamous papillomas of the esophagus are epithelial tumors considered to be benign. They are rare... more Squamous papillomas of the esophagus are epithelial tumors considered to be benign. They are rare, occurring in 0.01% of individuals at autopsy [1] and 0.07% of individuals in an endoscopy series [2]. Development of extensive esophageal squamous papillomas, otherwise known as squamous papillomatosis of the esophagus (ESP) is even less common [3]. We herein report a case with esophageal squamous papillomatosis.