Hussein Daood - Academia.edu (original) (raw)
Papers by Hussein Daood
Although organic farming is rapidly expanding in the vineyards of southern Europe, conventional c... more Although organic farming is rapidly expanding in the vineyards of southern Europe, conventional crop management, using treatments that require a number of chemical inputs to guarantee yields is still the most common approach to crop management. To gauge the effects of these management systems on biodiversity, communities of vascular plants, butterflies, moths and birds were studied in vineyards in the Priorat Appellation of Origin (Catalonia, NE Spain). Measurements inside plots (all four taxonomic groups) and in grass strips between crop lines (only butterflies and vascular plants) were taken in organically and non-organically treated vineyards. Crop treatment was found to have an important effect, stronger on the most sessile organisms. Organic farms hosted consistently richer communities of both vascular plants and butterflies, a trend that was also observed − albeit less significantly − in moths. The weaker response in this group was probably due to insufficient sampling. Birds, the most vagile of the surveyed taxa, showed no significant response to treatments. Grass strips acted in all cases as reservoirs of biodiversity and hosted richer assemblages. The current trend of placing vineyards on slopes without terracing should ensure the existence of uncultivated strips within the vineyards to enhance the biodiversity of these agroecosystems. As well, parameters such as altitude and urban surface area are important drivers of biodiversity in this region. Our results suggest that organic farming may contribute to halting the widespread decrease that is occurring in communities of butterflies and other insects in this region.
Journal of Supercritical Fluids, Aug 1, 1997
Extraction of whole fruit, seeds and peel of hiprose were studied with carbon dioxide, propane + ... more Extraction of whole fruit, seeds and peel of hiprose were studied with carbon dioxide, propane + carbon dioxide and propane as solvents under super- and subcritical conditions. The percentage of extract recovered from seed, whole hipfruit and hippeel was found to be 5.7–6.7, 3.0–3.5 and 0.32–0.42, respectively. The ratios of solvent to plant material, required to attain a complete extraction,
Agrokémia és Talajtan, 2000
Acta Alimentaria, Mar 1, 2011
This work was conducted to study the changes in the content and composition of chlorophylls and c... more This work was conducted to study the changes in the content and composition of chlorophylls and carotenoids in ground thyme leaves as a function of supercritical carbon dioxide and sub-critical propane extraction. the results indicated that the maximum oleoresin yield could be obtained with supercritical carbon dioxide at 400 bar and 35 or 55 °C and sub-critical propane at 50 bar. Pigment solvating capacity of supercritical carbon dioxide increased with the increase of extraction pressure. Substantial variation was noticed in pigment composition between raw material, oleoresin and residues (powders after extraction). Chlorophyll b, chlorophyll a and the Mg-free derivatives were the dominant pigments in the raw material with other derivatives being minor constituents. After extraction with supercritical carbon dioxide at 35 °C and pressures higher than 200 bar, epimerisation and oxidation of chlorophylls lead to marked accumulation of artefacts in the residues. The content of oxidized chlorophylls was increased proportionally to the increase of the extraction pressure. The brownish green coloured-oleoresins contained only pheophytins indicating that rapid substitution of Mg with hydrogen atoms on the molecules takes place during extraction of such pigment by supercritical carbon dioxide and sub-critical propane. The marked conversion of chlorophyll to pheophytins was found in oleoresin extracted by supercritical fluid extraction at 55 °C and 100 bar.
A new analytical procedure for the simultaneous determination of L-ascorbic acid (AA), isoascorbi... more A new analytical procedure for the simultaneous determination of L-ascorbic acid (AA), isoascorbic acid (IAA), L-dehydroascorbic acid (DHAA), and isodehydroascorbic acid (IDHAA) in food by high-performance liquid chromatography (HPLC) is developed. After separation on an HPLC column, an in-line oxidation of AA and IAA to DHAA and IDHAA, respectively, is performed on a short column of activated charcoal. The dehydroascorbic acids are derivatized with a 1,2-phenylenediamine solution in a heated capillary Tefzel reactor into fluorescent quinoxaline compounds and monitored fluorometrically. The chromatographic method provides good separation of LAA, LDHAA, and their diastereoisomers in a relatively short time (-10 min). After optimization of postcolumn derivatization conditions, calibration runs and recovery tests are performed. The fluorescent response in terms of peak area is highly proportional to the concentration of all derivatives examined over a range of 0.1 to 100 microg/mL solution for LAA, LDHAA, IAA, and IDHAA. Recoveries were in the range of 97 to 103%. The detection limit is 0.1 mg of each ascorbic acid derivative per 100 g food. A wide variety of foods (fruits, fruit juices, vegetables, vegetable products, milk, liver, and sausage) are analyzed by the developed procedure. The Vitamin C (LAA and LDHA) contents determined according to the present analytical method are in the same order of magnitude as the result of precolumn derivatization and the fluorometric methods. The described method is a highly specific procedure for determining Vitamin C in food. It is simple to handle, only slightly susceptible to disturbance, perfectly suitable for serial determinations, and yields reproducible results.
Journal of Supercritical Fluids, Apr 1, 2000
Oil extraction from coriander seed was studied with carbon dioxide and propane as solvents, under... more Oil extraction from coriander seed was studied with carbon dioxide and propane as solvents, under sub-and supercritical conditions. The ratio of solvent to seed (g/g) required to achieve a complete oil extraction was between 20 and 40 using CO 2 at pressures of 200 and 300 bar and temperature of 35°C. A complete oil recovery could be attained with propane or propane-rich solvents at 25°C and 50, 80 and 100 bar. The ratio of solvent to seed needed to approach maximum extraction was varied between 1.3 and 2.5. The solvating power of propane and propane-containing solvents was proved to be much higher than that of CO 2. The fractions trapped in the initial period of extraction with CO 2 were rich in essential oils. It was stated that the content and composition of essential oils in the extracts can be influenced by modifying the solvent composition and changing the extraction conditions, which had negligible effect on the fatty acid composition of the recovered oils. The changes in the tocopherol content of the extracts as a function of supercritical fluid extraction (SFE) conditions are also investigated in the present work.
Acta Alimentaria, Jun 1, 2020
Tomatoes (Solanum lycopersicon L.) are one of the most important and most widely consumed vegetab... more Tomatoes (Solanum lycopersicon L.) are one of the most important and most widely consumed vegetables in the world. The fruit contains considerable amount of diff erent phytonutrients such as carotenoids, tocopherols, and vitamin C. In the present work, eff ects of some abiotic factors on the concentration of phytonutrients were investigated in tomato cultivated in two diff erent types of soil. It was found that the type of soil had slight eff ect on the most important vital nutrients, while the ecological factors, particularly precipitation and average temperature 3 weeks before harvest, were of signifi cant infl uence on such nutrients. It was found that low temperature and high precipitation before harvest caused the levels of carotenoids, tocopherol, and vitamin C to signifi cantly increase by 65%, 46%, and 28%, respectively.
Systematic and Applied Microbiology
Journal of Agricultural and Food Chemistry, 1999
A study was conducted to investigate the change in quality attributes of red pepper (paprika) (Ca... more A study was conducted to investigate the change in quality attributes of red pepper (paprika) (Capsicum annuum L. var. Km-622) as a function of ripening and some technological factors. Of quality attributes, carotenoids and bioantioxidants (ascorbic acid and tocopherols) have been studied. It was found that the dynamics of fruit ripening with regard to carotenoids and bioantioxidants was influenced to a considerable extent by weather conditions of the production season. A rainy and cool season yielded fruits with more-carotene but less diesters of red xanthophylls as compared to those produced in a relatively dry and warm season. The ripening stage at harvest was found to affect the quality of paprika. Harvest at unripe stages (color break or faint red) resulted in a high accumulation of dehydroascorbic acid in the overripe fruits, whereas de novo biosynthesis of carotenoids and tocopherols was partially retarded. Application of pre-drying centrifugation resulted in a marked loss of ascorbic acid, and as a consequence, carotenoid stability was impaired during the storage of ground paprika. Sugar caramelization caused dry pods and ground paprika to retain more pigments and tocopherol as compared to those from control or centrifuged red pepper samples. During the storage of ground paprika, color stability was improved by grinding the seeds with the pericarp.
Zywnosc.Nauka.Technologia.Jakosc/Food.Science.Technology.Quality, 2014
S t r e s z c z e n i e Celem pracy było porównanie zawartości ośmiu wielopierścieniowych węglowo... more S t r e s z c z e n i e Celem pracy było porównanie zawartości ośmiu wielopierścieniowych węglowodorów aromatycznych w mięsie pstrągów tęczowych (Oncorhynchus mykiss), wędzonych metodą tradycyjną, z surowca świeżego i rozmrożonego, z ich zawartością w rynkowych rybach wędzonych różnymi metodami (w wędzarni z zewnętrzną wytwornicą dymu, tradycyjnie i przy użyciu preparatu dymu wędzarniczego). Oznaczenie WWA wykonano metodą wysokosprawnej chromatografii cieczowej z detektorem fluorescencyjnym. Maksymalne dopuszczalne stężenia badanych WWA w mięsie ryb wędzonych różnymi metodami nie zostały przekroczone w żadnej z analizowanych próbek, a średnia zawartość wahała się w granicach od < 0,30 μg/kg mięsa do 1,03 μg/kg mięsa, natomiast ΣWWA4 (benzo(a)piren + chryzen + benzo(a)antracen + benzo(b)fluoranten) nie przekroczyła 5,00 μg/kg mięsa. Analiza mięsa pstrągów tęczowych dostępnych na rynku lokalnym wykazała większą zawartość wybranych WWA w rybach wędzonych tradycyjnie niż w rybach wędzonych współczesnymi metodami. Średnia zawartość benzo[a]pirenu w mięsie pstrąga tęczowego wędzonego tradycyjnie z surowca świeżego wahała się od < 0,30 μg/kg do 0,57 μg/kg mięsa, w rybach wędzonych z surowca rozmrożonego od < 0,30 μg/kg do 0,70 μg/kg mięsa, natomiast największą zawartość benzo[a]pirenu oznaczono w rybach zakupionych w zakładach przetwórczych, deklarowanych jako wędzone tradycyjnie-1,03 μg/kg mięsa. Na podstawie badań stwierdzono, że zawartość WWA w mięsie wędzonego pstrąga tęczowego, niezależnie od zastosowanej metody produkcji, nie stanowi zagrożenia dla zdrowia konsumentów i nie zmniejsza żywieniowych wartości tej ryby. Słowa kluczowe: pstrąg tęczowy (Oncorhynchus mykiss), wędzenie różnymi metodami, wybrane WWA, najwyższe dopuszczalne poziomy WWA
Physiology, Biochemistry and Molecular Biology of Plant Lipids, 1997
Titanium is a widely distributed metal and ranks tenth among the most common elements in the eart... more Titanium is a widely distributed metal and ranks tenth among the most common elements in the earth crust. In the soils, titanium is generally found in the form of titanium dioxide or titanium silicates, and due to its low solubility is practically unavailable for plants. The application of soluble forms of titanium, Ti-chloride, Ti-sulphate or Ti-ascorbate (Titavit), has been proved to be beneficial to the growth and quality of some important agricultural crops [ 1, 2].
Physiology, Biochemistry and Molecular Biology of Plant Lipids, 1997
Several studies on irradiation of fruits and vegetables have been conducted to delay fruit ripene... more Several studies on irradiation of fruits and vegetables have been conducted to delay fruit ripeness, thus, their shelf life might be extended by few days to few weeks [1]. However in some horticultural crops gamma irradiation treatments initiated the climacteric ripeness sequences by inducing the preclimacteric fruits to produce stimulatory amount of ethylene [2]. Factors most likely to affect response of fruit and vegetables to irradiation may include type of fruit (climacteric or nonclimacteric), ripeness stage at expossure, dose of irradiation and post-treatment storage conditions.
Journal of the Science of Food and Agriculture, 2001
This work was conducted to investigate changes in the bioactive nutrient content of hot (pungent)... more This work was conducted to investigate changes in the bioactive nutrient content of hot (pungent) paprika as a function of ripening in four of the most important varieties cultivated in Hungary. For the quantitative determination, recent liquid chromatographic (HPLC) methods were applied. Forty carotenoids were separated, with β‐carotene and lutein of interest from the biological point of view. The major component of vitamin E was α‐tocopherol. Capsaicin, dihydrocapsaicin and nordihydrocapsaicin were the dominant pungency principles. As for vitamin C components, only ascorbic acid could be detected and determined. The results indicated that all the examined compounds were at low level in the mature green fruits, and the onset of climacteric ripening caused their content to grow. In accord with the advance in ripening, carotenoids were being formed even at the overripening stage, while tocopherols, capsaicinoids and ascorbic acid reached their maximum level at the colour break or red...
Journal of Plant Nutrition, 1988
Lipoxygenase was extracted from untreated tomato fruits and those treated with titanium ascorbate... more Lipoxygenase was extracted from untreated tomato fruits and those treated with titanium ascorbate (TITAVIT) at the last stage of ripening. It was found that titanium (Ti) had a activating effect on lipoxygenase so that the development of red pigments was inhibited to some extent. The addition of Titavit to the reaction mixture resulted in a lag period of about 1
Journal of Agricultural and Food Chemistry, 2001
Ground paprika (Capsicum annuum L.) was extracted with supercritical carbon dioxide (SC-CO 2) and... more Ground paprika (Capsicum annuum L.) was extracted with supercritical carbon dioxide (SC-CO 2) and subcritical propane at different conditions of pressure and temperature to estimate the yield and variation in carotenoid, tocopherol, and capsaicinoid contents and composition. The yield of paprika extract was found to be affected by the extraction conditions with SC-CO 2 but fairly constant at different conditions with subcritical propane. The maximum yields of oleoresin were 7.9 and 8.1% of ground paprika by SC-CO 2 and subcritical propane, respectively. The quantitative distribution of carotenoids, tocopherols, and capsaicinoids between paprika extract and powder was influenced by extraction conditions. SC-CO 2 was inefficient in the extraction of diesters of xanthophylls even at 400 bar and 55°C, whereas tocopherols and capsaicinoids were easy to extract at these conditions. Under mild conditions subcritical propane was superior to SC-CO 2 in the extraction of carotenoids and tocopherols but less efficient in the extraction of capsaicinoids.
Environmental Science and Pollution Research
Diclofenac, ibuprofen, and carbamazepine are three of the most widely detected and most concernin... more Diclofenac, ibuprofen, and carbamazepine are three of the most widely detected and most concerning pharmaceutical residues in aquatic ecosystems. The aim of this study was to identify bacteria that may be involved in their degradation from a bacterial biofilm. Selective enrichment cultures in mineral salt solution containing pharmaceutical compounds as sole source of carbon and energy were set up, and population dynamics were monitored using shotgun metagenome sequencing. Bacterial genomes were reconstructed using genome-resolved metagenomics. Thirty bacterial isolates were obtained, identified at species level, and tested regarding pharmaceutical biodegradation at an initial concentration of 1.5 mg l−1. The results indicated that most probably diclofenac biodegrading cultures consisted of members of genera Ferrovibrio, Hydrocarboniphaga, Zavarzinia, and Sphingopyxis, while in ibuprofen biodegradation Nocardioides and Starkeya, and in carbamazepine biodegradation Nocardioides, Pseud...
International journal of horticultural science, Jan 3, 2010
The goal of the present work was to compare different sweet and sour cherry cultivars and cultiva... more The goal of the present work was to compare different sweet and sour cherry cultivars and cultivation methods (bio/integrated) with respect to polyphenol content and antioxidant activity. The concentration of total polyphenols ranged between 880-1050 mg kg-1 of fresh fruit, whereas antioxidant activity expressed as TEAC was found to be between 5.4 and 10.3 mmol kg-1 for the sweet cherry cultivars examined. In case of sour cherry the level of polyphenols ranged between 1283 and 3490 mg/kg fresh edible part of the fruit. Antioxidant activity was recorded between 15-32 mmol kg-1 for the different sour cherry cultivars included in this work. After one-month storage at low temperature, the total phenols and antioxidant activity decreased by 2-40% in the sour cherry cultivars studied. The anthocyanin content in cherry cultivars was less (131-312 mg kg-1) than the135-1893 mg kg-1 found in sour cherries. Anthocyanin level was higher in samples produced under organic farming conditions than in those produced with integrated cultivation.
Although organic farming is rapidly expanding in the vineyards of southern Europe, conventional c... more Although organic farming is rapidly expanding in the vineyards of southern Europe, conventional crop management, using treatments that require a number of chemical inputs to guarantee yields is still the most common approach to crop management. To gauge the effects of these management systems on biodiversity, communities of vascular plants, butterflies, moths and birds were studied in vineyards in the Priorat Appellation of Origin (Catalonia, NE Spain). Measurements inside plots (all four taxonomic groups) and in grass strips between crop lines (only butterflies and vascular plants) were taken in organically and non-organically treated vineyards. Crop treatment was found to have an important effect, stronger on the most sessile organisms. Organic farms hosted consistently richer communities of both vascular plants and butterflies, a trend that was also observed − albeit less significantly − in moths. The weaker response in this group was probably due to insufficient sampling. Birds, the most vagile of the surveyed taxa, showed no significant response to treatments. Grass strips acted in all cases as reservoirs of biodiversity and hosted richer assemblages. The current trend of placing vineyards on slopes without terracing should ensure the existence of uncultivated strips within the vineyards to enhance the biodiversity of these agroecosystems. As well, parameters such as altitude and urban surface area are important drivers of biodiversity in this region. Our results suggest that organic farming may contribute to halting the widespread decrease that is occurring in communities of butterflies and other insects in this region.
Journal of Supercritical Fluids, Aug 1, 1997
Extraction of whole fruit, seeds and peel of hiprose were studied with carbon dioxide, propane + ... more Extraction of whole fruit, seeds and peel of hiprose were studied with carbon dioxide, propane + carbon dioxide and propane as solvents under super- and subcritical conditions. The percentage of extract recovered from seed, whole hipfruit and hippeel was found to be 5.7–6.7, 3.0–3.5 and 0.32–0.42, respectively. The ratios of solvent to plant material, required to attain a complete extraction,
Agrokémia és Talajtan, 2000
Acta Alimentaria, Mar 1, 2011
This work was conducted to study the changes in the content and composition of chlorophylls and c... more This work was conducted to study the changes in the content and composition of chlorophylls and carotenoids in ground thyme leaves as a function of supercritical carbon dioxide and sub-critical propane extraction. the results indicated that the maximum oleoresin yield could be obtained with supercritical carbon dioxide at 400 bar and 35 or 55 °C and sub-critical propane at 50 bar. Pigment solvating capacity of supercritical carbon dioxide increased with the increase of extraction pressure. Substantial variation was noticed in pigment composition between raw material, oleoresin and residues (powders after extraction). Chlorophyll b, chlorophyll a and the Mg-free derivatives were the dominant pigments in the raw material with other derivatives being minor constituents. After extraction with supercritical carbon dioxide at 35 °C and pressures higher than 200 bar, epimerisation and oxidation of chlorophylls lead to marked accumulation of artefacts in the residues. The content of oxidized chlorophylls was increased proportionally to the increase of the extraction pressure. The brownish green coloured-oleoresins contained only pheophytins indicating that rapid substitution of Mg with hydrogen atoms on the molecules takes place during extraction of such pigment by supercritical carbon dioxide and sub-critical propane. The marked conversion of chlorophyll to pheophytins was found in oleoresin extracted by supercritical fluid extraction at 55 °C and 100 bar.
A new analytical procedure for the simultaneous determination of L-ascorbic acid (AA), isoascorbi... more A new analytical procedure for the simultaneous determination of L-ascorbic acid (AA), isoascorbic acid (IAA), L-dehydroascorbic acid (DHAA), and isodehydroascorbic acid (IDHAA) in food by high-performance liquid chromatography (HPLC) is developed. After separation on an HPLC column, an in-line oxidation of AA and IAA to DHAA and IDHAA, respectively, is performed on a short column of activated charcoal. The dehydroascorbic acids are derivatized with a 1,2-phenylenediamine solution in a heated capillary Tefzel reactor into fluorescent quinoxaline compounds and monitored fluorometrically. The chromatographic method provides good separation of LAA, LDHAA, and their diastereoisomers in a relatively short time (-10 min). After optimization of postcolumn derivatization conditions, calibration runs and recovery tests are performed. The fluorescent response in terms of peak area is highly proportional to the concentration of all derivatives examined over a range of 0.1 to 100 microg/mL solution for LAA, LDHAA, IAA, and IDHAA. Recoveries were in the range of 97 to 103%. The detection limit is 0.1 mg of each ascorbic acid derivative per 100 g food. A wide variety of foods (fruits, fruit juices, vegetables, vegetable products, milk, liver, and sausage) are analyzed by the developed procedure. The Vitamin C (LAA and LDHA) contents determined according to the present analytical method are in the same order of magnitude as the result of precolumn derivatization and the fluorometric methods. The described method is a highly specific procedure for determining Vitamin C in food. It is simple to handle, only slightly susceptible to disturbance, perfectly suitable for serial determinations, and yields reproducible results.
Journal of Supercritical Fluids, Apr 1, 2000
Oil extraction from coriander seed was studied with carbon dioxide and propane as solvents, under... more Oil extraction from coriander seed was studied with carbon dioxide and propane as solvents, under sub-and supercritical conditions. The ratio of solvent to seed (g/g) required to achieve a complete oil extraction was between 20 and 40 using CO 2 at pressures of 200 and 300 bar and temperature of 35°C. A complete oil recovery could be attained with propane or propane-rich solvents at 25°C and 50, 80 and 100 bar. The ratio of solvent to seed needed to approach maximum extraction was varied between 1.3 and 2.5. The solvating power of propane and propane-containing solvents was proved to be much higher than that of CO 2. The fractions trapped in the initial period of extraction with CO 2 were rich in essential oils. It was stated that the content and composition of essential oils in the extracts can be influenced by modifying the solvent composition and changing the extraction conditions, which had negligible effect on the fatty acid composition of the recovered oils. The changes in the tocopherol content of the extracts as a function of supercritical fluid extraction (SFE) conditions are also investigated in the present work.
Acta Alimentaria, Jun 1, 2020
Tomatoes (Solanum lycopersicon L.) are one of the most important and most widely consumed vegetab... more Tomatoes (Solanum lycopersicon L.) are one of the most important and most widely consumed vegetables in the world. The fruit contains considerable amount of diff erent phytonutrients such as carotenoids, tocopherols, and vitamin C. In the present work, eff ects of some abiotic factors on the concentration of phytonutrients were investigated in tomato cultivated in two diff erent types of soil. It was found that the type of soil had slight eff ect on the most important vital nutrients, while the ecological factors, particularly precipitation and average temperature 3 weeks before harvest, were of signifi cant infl uence on such nutrients. It was found that low temperature and high precipitation before harvest caused the levels of carotenoids, tocopherol, and vitamin C to signifi cantly increase by 65%, 46%, and 28%, respectively.
Systematic and Applied Microbiology
Journal of Agricultural and Food Chemistry, 1999
A study was conducted to investigate the change in quality attributes of red pepper (paprika) (Ca... more A study was conducted to investigate the change in quality attributes of red pepper (paprika) (Capsicum annuum L. var. Km-622) as a function of ripening and some technological factors. Of quality attributes, carotenoids and bioantioxidants (ascorbic acid and tocopherols) have been studied. It was found that the dynamics of fruit ripening with regard to carotenoids and bioantioxidants was influenced to a considerable extent by weather conditions of the production season. A rainy and cool season yielded fruits with more-carotene but less diesters of red xanthophylls as compared to those produced in a relatively dry and warm season. The ripening stage at harvest was found to affect the quality of paprika. Harvest at unripe stages (color break or faint red) resulted in a high accumulation of dehydroascorbic acid in the overripe fruits, whereas de novo biosynthesis of carotenoids and tocopherols was partially retarded. Application of pre-drying centrifugation resulted in a marked loss of ascorbic acid, and as a consequence, carotenoid stability was impaired during the storage of ground paprika. Sugar caramelization caused dry pods and ground paprika to retain more pigments and tocopherol as compared to those from control or centrifuged red pepper samples. During the storage of ground paprika, color stability was improved by grinding the seeds with the pericarp.
Zywnosc.Nauka.Technologia.Jakosc/Food.Science.Technology.Quality, 2014
S t r e s z c z e n i e Celem pracy było porównanie zawartości ośmiu wielopierścieniowych węglowo... more S t r e s z c z e n i e Celem pracy było porównanie zawartości ośmiu wielopierścieniowych węglowodorów aromatycznych w mięsie pstrągów tęczowych (Oncorhynchus mykiss), wędzonych metodą tradycyjną, z surowca świeżego i rozmrożonego, z ich zawartością w rynkowych rybach wędzonych różnymi metodami (w wędzarni z zewnętrzną wytwornicą dymu, tradycyjnie i przy użyciu preparatu dymu wędzarniczego). Oznaczenie WWA wykonano metodą wysokosprawnej chromatografii cieczowej z detektorem fluorescencyjnym. Maksymalne dopuszczalne stężenia badanych WWA w mięsie ryb wędzonych różnymi metodami nie zostały przekroczone w żadnej z analizowanych próbek, a średnia zawartość wahała się w granicach od < 0,30 μg/kg mięsa do 1,03 μg/kg mięsa, natomiast ΣWWA4 (benzo(a)piren + chryzen + benzo(a)antracen + benzo(b)fluoranten) nie przekroczyła 5,00 μg/kg mięsa. Analiza mięsa pstrągów tęczowych dostępnych na rynku lokalnym wykazała większą zawartość wybranych WWA w rybach wędzonych tradycyjnie niż w rybach wędzonych współczesnymi metodami. Średnia zawartość benzo[a]pirenu w mięsie pstrąga tęczowego wędzonego tradycyjnie z surowca świeżego wahała się od < 0,30 μg/kg do 0,57 μg/kg mięsa, w rybach wędzonych z surowca rozmrożonego od < 0,30 μg/kg do 0,70 μg/kg mięsa, natomiast największą zawartość benzo[a]pirenu oznaczono w rybach zakupionych w zakładach przetwórczych, deklarowanych jako wędzone tradycyjnie-1,03 μg/kg mięsa. Na podstawie badań stwierdzono, że zawartość WWA w mięsie wędzonego pstrąga tęczowego, niezależnie od zastosowanej metody produkcji, nie stanowi zagrożenia dla zdrowia konsumentów i nie zmniejsza żywieniowych wartości tej ryby. Słowa kluczowe: pstrąg tęczowy (Oncorhynchus mykiss), wędzenie różnymi metodami, wybrane WWA, najwyższe dopuszczalne poziomy WWA
Physiology, Biochemistry and Molecular Biology of Plant Lipids, 1997
Titanium is a widely distributed metal and ranks tenth among the most common elements in the eart... more Titanium is a widely distributed metal and ranks tenth among the most common elements in the earth crust. In the soils, titanium is generally found in the form of titanium dioxide or titanium silicates, and due to its low solubility is practically unavailable for plants. The application of soluble forms of titanium, Ti-chloride, Ti-sulphate or Ti-ascorbate (Titavit), has been proved to be beneficial to the growth and quality of some important agricultural crops [ 1, 2].
Physiology, Biochemistry and Molecular Biology of Plant Lipids, 1997
Several studies on irradiation of fruits and vegetables have been conducted to delay fruit ripene... more Several studies on irradiation of fruits and vegetables have been conducted to delay fruit ripeness, thus, their shelf life might be extended by few days to few weeks [1]. However in some horticultural crops gamma irradiation treatments initiated the climacteric ripeness sequences by inducing the preclimacteric fruits to produce stimulatory amount of ethylene [2]. Factors most likely to affect response of fruit and vegetables to irradiation may include type of fruit (climacteric or nonclimacteric), ripeness stage at expossure, dose of irradiation and post-treatment storage conditions.
Journal of the Science of Food and Agriculture, 2001
This work was conducted to investigate changes in the bioactive nutrient content of hot (pungent)... more This work was conducted to investigate changes in the bioactive nutrient content of hot (pungent) paprika as a function of ripening in four of the most important varieties cultivated in Hungary. For the quantitative determination, recent liquid chromatographic (HPLC) methods were applied. Forty carotenoids were separated, with β‐carotene and lutein of interest from the biological point of view. The major component of vitamin E was α‐tocopherol. Capsaicin, dihydrocapsaicin and nordihydrocapsaicin were the dominant pungency principles. As for vitamin C components, only ascorbic acid could be detected and determined. The results indicated that all the examined compounds were at low level in the mature green fruits, and the onset of climacteric ripening caused their content to grow. In accord with the advance in ripening, carotenoids were being formed even at the overripening stage, while tocopherols, capsaicinoids and ascorbic acid reached their maximum level at the colour break or red...
Journal of Plant Nutrition, 1988
Lipoxygenase was extracted from untreated tomato fruits and those treated with titanium ascorbate... more Lipoxygenase was extracted from untreated tomato fruits and those treated with titanium ascorbate (TITAVIT) at the last stage of ripening. It was found that titanium (Ti) had a activating effect on lipoxygenase so that the development of red pigments was inhibited to some extent. The addition of Titavit to the reaction mixture resulted in a lag period of about 1
Journal of Agricultural and Food Chemistry, 2001
Ground paprika (Capsicum annuum L.) was extracted with supercritical carbon dioxide (SC-CO 2) and... more Ground paprika (Capsicum annuum L.) was extracted with supercritical carbon dioxide (SC-CO 2) and subcritical propane at different conditions of pressure and temperature to estimate the yield and variation in carotenoid, tocopherol, and capsaicinoid contents and composition. The yield of paprika extract was found to be affected by the extraction conditions with SC-CO 2 but fairly constant at different conditions with subcritical propane. The maximum yields of oleoresin were 7.9 and 8.1% of ground paprika by SC-CO 2 and subcritical propane, respectively. The quantitative distribution of carotenoids, tocopherols, and capsaicinoids between paprika extract and powder was influenced by extraction conditions. SC-CO 2 was inefficient in the extraction of diesters of xanthophylls even at 400 bar and 55°C, whereas tocopherols and capsaicinoids were easy to extract at these conditions. Under mild conditions subcritical propane was superior to SC-CO 2 in the extraction of carotenoids and tocopherols but less efficient in the extraction of capsaicinoids.
Environmental Science and Pollution Research
Diclofenac, ibuprofen, and carbamazepine are three of the most widely detected and most concernin... more Diclofenac, ibuprofen, and carbamazepine are three of the most widely detected and most concerning pharmaceutical residues in aquatic ecosystems. The aim of this study was to identify bacteria that may be involved in their degradation from a bacterial biofilm. Selective enrichment cultures in mineral salt solution containing pharmaceutical compounds as sole source of carbon and energy were set up, and population dynamics were monitored using shotgun metagenome sequencing. Bacterial genomes were reconstructed using genome-resolved metagenomics. Thirty bacterial isolates were obtained, identified at species level, and tested regarding pharmaceutical biodegradation at an initial concentration of 1.5 mg l−1. The results indicated that most probably diclofenac biodegrading cultures consisted of members of genera Ferrovibrio, Hydrocarboniphaga, Zavarzinia, and Sphingopyxis, while in ibuprofen biodegradation Nocardioides and Starkeya, and in carbamazepine biodegradation Nocardioides, Pseud...
International journal of horticultural science, Jan 3, 2010
The goal of the present work was to compare different sweet and sour cherry cultivars and cultiva... more The goal of the present work was to compare different sweet and sour cherry cultivars and cultivation methods (bio/integrated) with respect to polyphenol content and antioxidant activity. The concentration of total polyphenols ranged between 880-1050 mg kg-1 of fresh fruit, whereas antioxidant activity expressed as TEAC was found to be between 5.4 and 10.3 mmol kg-1 for the sweet cherry cultivars examined. In case of sour cherry the level of polyphenols ranged between 1283 and 3490 mg/kg fresh edible part of the fruit. Antioxidant activity was recorded between 15-32 mmol kg-1 for the different sour cherry cultivars included in this work. After one-month storage at low temperature, the total phenols and antioxidant activity decreased by 2-40% in the sour cherry cultivars studied. The anthocyanin content in cherry cultivars was less (131-312 mg kg-1) than the135-1893 mg kg-1 found in sour cherries. Anthocyanin level was higher in samples produced under organic farming conditions than in those produced with integrated cultivation.