Hyeung-jin Jang - Academia.edu (original) (raw)

Papers by Hyeung-jin Jang

Colorectal cancer (CRC) is the third most commonly diagnosed cancer worldwide, and despite advanc... more Colorectal cancer (CRC) is the third most commonly diagnosed cancer worldwide, and despite advances in treatment, survival rates are still low; therefore, the development of novel drugs is imperative. Acetylcorynoline (ACN) is derived from Corydalis ambigua. The effect of ACN on colon cancer is still unknown. Therefore, we investigated its potential effects. Our data showed that ACN inhibited cell viability and proliferation. Moreover, ACN induced apoptosis and cell cycle arrest by inhibiting cell growth. In the present study, we hypothesized that ACN regulates c-Myc through CNOT2 or MID1IP1. ACN reduced the protein expression of oncogenic genes, decreased c-Myc half-life, and rapidly inhibited the serum stimulation response. Moreover, knockdown of CNOT2 and MID1IP1 with ACN increased apoptosis and further reduced the expression of oncogenes. In addition, ACN exhibited a synergistic effect with low-dose of 5-fluorouracil (5-FU) and doxorubicin (Dox). Collectively, our data demonstra...

46th Annual Meeting, Oct 25, 2008

• Group VII: : genes repressed upon 20 min and imduced upon 60 min exposure (3 genes)-Intriguingl... more • Group VII: : genes repressed upon 20 min and imduced upon 60 min exposure (3 genes)-Intriguingly, we observed that SA2459, SA2460 and SA2461 (Intriguingly, we observed that SA2459, SA2460 and SA2461 (ica icaADB ADB) which) which make up the intercellular adhesion (make up the intercellular adhesion (ica ica)) operon operon and contribute to virulence in and contribute to virulence in S. S. aureus aureus were were downregulated downregulated after 20 min and after 20 min and upregulated upregulated after 60 min of after 60 min of exposure to OPP. exposure to OPP.

Molecules, Apr 14, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

The Multi-Targeted Effects ofChrysanthemumHerb Extract AgainstEscherichia coliO157:H7

Phytotherapy Research, Dec 17, 2012

The Chrysanthemum lavandulifolium extract, which includes chrysoeriol, sudachitin, and acacetin, ... more The Chrysanthemum lavandulifolium extract, which includes chrysoeriol, sudachitin, and acacetin, has excellent antibiotic effects on Escherichia coli O157:H7 (E. coli O157). A notable point is that the antibiotic targets of the herb extract are similar to the targets of commonly used antibiotic drugs, including bacterial cell wall biosynthesis, bacterial protein synthesis, and bacterial DNA replication and repair. In addition, the herbal antibiotic inhibits the etiological factors that contribute to the pathogenic property. The herbal sample was extracted and fractionated and then inoculated through a disk diffusion method to confirm its antibiotic effect against E. coli O157. Total RNA was isolated from the affected bacterial cells, and its expression level was analyzed through a microarray analysis. To confirm the accuracy of the microarray data, a real‐time PCR was performed. Three active compounds, chrysoeriol, sudachitin, and acacetin, were identified with a high‐performance liquid chromatography‐electrospray ionization/mass spectrometry chromatogram, and the disk diffusion study confirmed that chrysoeriol and sudachitin contribute to the antibiotic properties of the herb extract. The results demonstrate that the multi‐target efficacy of the herbal sample may indicate the potential for the development of more effective and safer drugs that will act as substitutes for existing antibiotics. Copyright © 2012 John Wiley & Sons, Ltd.

Biomedicine & Pharmacotherapy, Apr 1, 2017

Non-alcoholic fatty liver disease (NAFLD) is one of the most common diseases worldwide and has co... more Non-alcoholic fatty liver disease (NAFLD) is one of the most common diseases worldwide and has continuously increased. NAFLD refers to a spectrum of diseases ranging from fatty liver to steatohepatitis, cirrhosis, and even to hepatocyte carcinoma. Excessive fatty acid enters the cell and the mitochondria undergo stress and unremoved ROS can trigger a form of cell apoptosis known as 'lipoapoptosis'. NASH arises from damaged liver hepatocytes due to lipotoxicity. NASH not only involves lipid accumulation and apoptosis but also inflammation. Ginkgo biloba has been tested clinical trials as a traditional medicine for asthma, bronchitis and cardiovascular disease. The effects of Ginkgolide A (GA), derived from the ginkgo biloba leaf, are still unknown in NAFLD. To determine the protective effects of GA in NAFLD, we examined the fatty liver disease condition in the non-esterified fatty acid (NEFA)-induced HepG2 cell line and in a high fat diet mouse model. The findings of this study suggest that GA is non-toxic at high concentrations in hepatocytes. Moreover, GA was found to inhibit cellular lipogenesis and lipid accumulation by causing mitochondrial oxidative stress. GA showed hepatoprotective efficacy by inducing cellular lipoapoptosis and by inhibiting cellular inflammation. The results demonstrated that GA may be feasible as a therapeutic agent for NAFLD patients.

Journal of Ethnopharmacology, Aug 1, 2015

, A bitter herbal medicine Gentiana scabra root extract stimulates glucagon-like peptide-1 secret... more , A bitter herbal medicine Gentiana scabra root extract stimulates glucagon-like peptide-1 secretion and regulates Blood glucose in Db/Db mouse.,

Chlorogenic acid ameliorates brain damage and edema by inhibiting matrix metalloproteinase-2 and 9 in a rat model of focal cerebral ischemia

European Journal of Pharmacology, Aug 1, 2012

Chlorogenic acid (CGA) has been reported to have various beneficial effects on the cardiovascular... more Chlorogenic acid (CGA) has been reported to have various beneficial effects on the cardiovascular and central nervous systems. The purpose of the current study was to investigate whether CGA has protective effects against cerebral ischemia and whether these effects are due to modification of brain edema-related vascular factors. In a rat model of transient middle cerebral artery occlusion (MCAo, 2h of occlusion followed by 22 h of reperfusion), we measured infarct volume and performed behavioral test to evaluate the effects of CGA on brain damage and sensory-motor functional deficits. Brain water content and Evans blue extravasation were measured to evaluate brain edema and blood brain barrier (BBB) damage. Lipid peroxidation (LPO) and the expressions and activities of matrix metalloproteinase (MMP)-2 and MMP-9 were measured to investigate the mechanisms of action. Intraperitoneal injection of CGA (3, 10, and 30 mg/kg) at 0 h and 2h after MCAo dose-dependently reduced infarct volume and sensory-motor functional deficits. It also reduced brain water content and Evans blue extravasation. Mechanistically, CGA reduced LPO and MMPs expressions and activities. These results suggest that CGA reduces brain damage, BBB damage and brain edema by radical scavenging activity and the inhibitory effects on MMP-2 and MMP-9.

The American Journal of Surgical Pathology, May 1, 2015

Matrix metalloproteinase-2 (gelatinase A) is a wellknown mediator of cancer metastasis, but it is... more Matrix metalloproteinase-2 (gelatinase A) is a wellknown mediator of cancer metastasis, but it is also thought to be involved in several aspects of cancer development, including cell growth and inflammation. In the present study, we investigate whether MMP-2 SNP, MMP-2 mRNAs, and MMP-2 protein are associated with the susceptibility to colorectal cancer in the Tunisian population. The TaqMan allele discrimination assay and DNA sequencing techniques were used for genotyping; MMP-2 expression of each genotype was analyzed by semiquantitative RT-PCR, and MMP-2 protein expression was analyzed by immunohistochemistry staining. Our result showed that the levels of MMP-2 mRNA expression in patients containing the CC genotype were much higher compared with cells with the CT genotype. The frequency of the MMP-2 CC genotype was significantly higher in colorectal cancer patients when compared with controls (OR = 1.94; 95% CI, 1.117-3.680). A higher intensity of staining of MMP-2 was observed in regions of invasion of the muscularis mucosa compared with superficial portions of the tumor. In addition, we found a significant progressive increase in total MMP-2 plasma levels with progression from adenomatous polyps through advancing Dukes stages (P = 0.0001). Our data suggest that MMP-2 may be associated with colorectal cancer development and invasion in the Tunisian population; moreover, SNP and levels of MMP-2 could be a predictive value for colorectal cancer prevention and invasiveness.

Archives of Pathology & Laboratory Medicine, Oct 3, 2022

Context.-Although several neuroendocrine cell types constitute gastroenteropancreatic neuroendocr... more Context.-Although several neuroendocrine cell types constitute gastroenteropancreatic neuroendocrine tumors (NETs), the clinical and prognostic implications of the expression of multiple peptide hormones have not been comprehensively evaluated in rectal NETs. Objective.-To identify the clinicopathologic characteristics and prognostic impact of peptide hormone expression. Design.-We evaluated the expression of peptide YY (PYY), glucagon, somatostatin, serotonin, insulin, and gastrin using immunolabeling in 446 endoscopically or surgically resected rectal NETs. Results.-PYY, glucagon, serotonin, somatostatin, insulin, and gastrin were expressed in 261 of 389 (67.1%), 205 of 446 (46.0%), 36 of 446 (8.1%), 33 of 446 (7.4%), 2 of 446 (0.4%), and 1 of 446 cases (0.2%), respectively. Immunoreactivity to any peptide hormone was present in 345 of 446 cases (77.4%). Tumors expressing serotonin or somatostatin were associated with lymphovascular invasion , chromogranin A expression, and shorter disease-free survival (DFS). Rectal NETs were classified as L-cell, enterochromaffin-cell, D-cell, null-expression, or mixedexpression type based on peptide hormonal expression status. Patients with D-cell NET had the shortest DFS (10year DFS, 54.5%), followed by those with enterochromaffin-cell NET (89.5%), null expression (97.0%), L-cell NET (99.6%), and mixed-expression NET (100%; P , .001). Multivariable analyses revealed that somatostatin expression was an independent indicator of poor prognosis with respect to DFS in rectal NETs (P ¼ .001). Conclusions.-Somatostatin expression is a poor prognostic indicator in patients with rectal NETs. Therefore, additional peptide hormonal immunolabeling, including somatostatin, serotonin, and PYY, in rectal NETs can provide more information regarding DFS.

Dyes and Pigments, Mar 1, 2019

A new wavelength-tunable and facilely functionable electron donor (D)-acceptor (A) type fluoresce... more A new wavelength-tunable and facilely functionable electron donor (D)-acceptor (A) type fluorescent naphthalene core skeleton and its application for cell membrane imaging are reported. The core skeleton shows bright green fluorescence with aldehyde(-CHO) moiety at the electron acceptor site, and its emission wavelength is tuned to blue and red by introducing the moiety of nitrile (-CN) and dicyanovinyl (-CH(CN) 2), respectively, at the acceptor site. In addition, the core skeleton can be readily functionable by introducing a targeting moiety at the extra hydroxyl group. For the first demonstration as a fluorescent probe, a long-chain carbon is introduced and evaluated its bio-imaging ability for the cellular membranes on cells and tissues by using confocal laser scanning microscopy (CLSM) and two-photon microscopy (TPM). The synthesis, photophysical property, and quantum chemical calculations are also identified.

International Journal of Molecular Sciences, Dec 2, 2022

Abstract 864: Ophiopogonin D increase apoptosis by activating p53 via ribosomal protein L5 and L11 and inhibiting the expression of c-Myc via CNOT2

Cancer Research, Jun 15, 2022

Extracted from the root tuber of Ophiopogon japonicas, ophiopogon is well known to have an anti-c... more Extracted from the root tuber of Ophiopogon japonicas, ophiopogon is well known to have an anti-cancer effect. However, the underlying mechanisms are still largely unknown. Here, we report that Ophiopogon D (OP-D) can inhibit colon cancer cell proliferation and induce apoptosis by inhibiting c-Myc expression through activation of p53 and CNOT2 regulation. Our results showed that OP-D induced p53 expression via ribosomal protein L5 or L11 and inhibited c-Myc expression through CNOT2 in a dose-dependent manner. Additionally, OP-D regulated cyclin D1 and CDK4 which are well known as cell cycle regulatory proteins. Consistently, OP-D inhibited the phosphorylation of AKT expression in a dose-dependent manner. Furthermore, OP-D shortened c-Myc’s half-life in a time-dependent manner. Furthermore, CNOT2 knockdown enhanced the inhibitory effect of OP-D on c-Myc in colon cancer cells. Interestingly, OP-D has increased the apoptotic effect of colon cancer cells when combined with doxorubicin or 5-FU, a treatment already used clinically. Altogether, our results suggested that OP-D regulates colon cancer cell survival and induces apoptosis by inhibiting c-Myc expression via activation of p53 and CNOT2 regulation. Citation Format: Hyun Min Ko, Wona Jee, Do-il Park, Somi Park, Ye-Rin Park, Hyeung-Jin Jang, Ji Hoon Jung. Ophiopogonin D increase apoptosis by activating p53 via ribosomal protein L5 and L11 and inhibiting the expression of c-Myc via CNOT2 [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 864.

Oldenlandia diffusa Ameliorates Dextran Sulphate Sodium-Induced Colitis Through Inhibition of NF-κB Activation

The American Journal of Chinese Medicine, 2011

Ulcerative colitis (UC) is an inflammatory bowel disease, which is a chronic gastrointestinal dis... more Ulcerative colitis (UC) is an inflammatory bowel disease, which is a chronic gastrointestinal disorder. Oldenlandia diffusa (OD) has been used as a traditional oriental medicine for inflammation. However, the regulatory effect and molecular mechanism of OD in intestinal inflammation are not yet understood. This study investigated the protective effect of OD in dextran sulfate sodium (DSS)-induced colitis. Mice treated with DSS showed remarkable clinical signs, including weight loss, and reduced colon length. Administration of OD attenuated these signs and significantly suppressed levels of interleukin (IL)-6, IL-1β and expression of cyclooxygenase-2 in DSS-treated colon tissues. OD also reduced the activation of transcription nuclear factor-κB p65 in DSS-treated colon tissues. Hentriacontane, a constituent of OD, attenuated weight loss, colon shortening, and levels of IL-6 caused by DSS. Taken together, the results provide experimental evidence that OD might be a useful therapeutic medicine for patients with UC.

Identification of allergens in Acorus gramineus using protein analysis

Molecular & Cellular Toxicology, Oct 24, 2022

Objectives This paper was aimed to review experimantal and clinical articles on Yeoldahanso-tang(... more Objectives This paper was aimed to review experimantal and clinical articles on Yeoldahanso-tang(YDHS), figuring out the recent tendency and suggesting the future prospects of YDHS-related studies. Methods The articles published in Korean or English since 2000 were searched, screened, and classified into experimental or clinical studies. And we structured designs, methods and results of the included studies into each table for the experimental studies or the clinical studies. Especially tools to diagnose constitutions and patterns identified in clinical researches were also summarized in the tables. Results 28 articles are finally included. 14 experimental studies are mostly in vitro designs to show efficacy of YDHS. There are only 2 studies for the safety. Among 14 clinical studies, 12 case studies target disorders of central nervous system, viral infection, skin ulcer and so on. 2 studies are before and after studies on cerebral infarction and effects on liver function. Conclusion...

$Research paper thumbnail of Inhibition of LPS-induced inflammatory biomarkers by ethyl acetate fraction ofPatrinia scabiosaefoliathrough suppression of NF-κB activation in RAW 264.7 cells$

Immunopharmacology and Immunotoxicology, 2011

Patrinia scabiosaefolia (PS) has been used for curing various types of inflammatory-related disor... more Patrinia scabiosaefolia (PS) has been used for curing various types of inflammatory-related disorders. However, the precise mechanism of the anti-inflammatory activity of PS remains unclear. Here, we investigated the anti-inflammatory effects of several fractions isolated from the PS in RAW 264.7 macrophages. The results indicated that the ethyl acetate fraction of PS (EAPS) concentration highly suppressed lipopolysaccharide (LPS)-induced nitric oxide (NO) and IL-6 productions without a cytotoxic effect on RAW 264.7 cells. EAPS inhibited the expressions of LPS-induced iNOS and COX-2 protein and their mRNA in a dose-dependent manner. Particularly, EAPS suppressed the level of nuclear factor-κB (NF-κB) activity, which was linked with the suppression of LPS-induced phosphorylation of p65 at serine 276 and p65 translocation into nuclei, but not MAPK signaling. In addition, treatment with eaPs inhibited the production of TNF-α in LPS-injected mice and suppressed the production of IL-6 and TNF-α in LPS-stimulated splenocytes from BALB/c mice. Therefore, we demonstrate here that Patrinia scabiosaefolia potentially inhibits the biomarkers related to inflammation through the blocking of NF-κB p65 activation, and it may be a potential therapeutic candidate for the treatment of inflammatory diseases.

Evidence-Based Complementary and Alternative Medicine, 2013

Obesity is a metabolic disorder characterized by chronic inflammation and dyslipidemia and is a s... more Obesity is a metabolic disorder characterized by chronic inflammation and dyslipidemia and is a strong predictor for the development of hypertension, diabetes mellitus, and cardiovascular disease. This study examined the antiobesity effect of an ethanol extract of Corni Fructus containing formulation (CDAP), which is a combination of four natural components: Corni Fructus, Dioscoreae Rhizoma, Aurantii Fructus Immaturus, and Platycodonis Radix. The cellular lipid content in 3T3-L1 adipocytes was assessed by Oil Red O staining. Expressions of peroxisome proliferator-activated receptor-γ(PPAR-γ), CCAAT/enhancer-binding protein-α(C/EBP-α), and lipin-1 were determined by real-time RT-PCR. Western blot was used to determine the protein levels of PPAR-γ, C/EBP-α, and AMP-activated protein kinase-α(AMPK-α). The CDAP extract suppressed the differentiation of 3T3-L1 adipocytes by downregulating cellular induction of PPAR-γ, C/EBP-α, and lipin-1. The CDAP extract also significantly upregulated...

Biochip Journal, 2011

The appearance of the super bacteria arose from mis-or abuse of antibiotics lead to the increasin... more The appearance of the super bacteria arose from mis-or abuse of antibiotics lead to the increasing interest about strong and safety new antibiotics. Angelica dahurica Bentham et Hooker (ADBH) is a herbal antibiotic drug which has been frequently prescribed in oriental pharmacology and has historically proven to be effective for bacterial infection. However, the molecular mechanism of the herbal antibiotic has not been cleared. Here, we examined the ADBH's antibiotic mechanism at the molecular level. We have focused on the simultaneous multi-target inhibitory effect of the of the ADBH ethyl acetate (EA) fraction on the enterohemorrhagic Escherichia coli O157:H7. Disk diffusion and DNA microarray was performed to investigate the molecular mechanism of the ADBH's antibiotic effect, and real-time quantitative PCR was employed to confirm the microarray data. The inhibitory effect of the ADBH EA fraction was on the bacterial cell envelope formation, folate biosynthesis, DNA replication and etiological factors of the E. coli O157:H7. This multi-target efficacy of the herbal antibiotic may be used as more effective and safer drugs that substitute existing antibiotics.

Anti-fibrotic effects of Kyungheechunggan-tang on activated hepatic stellate cells and rat liver

Molecular & Cellular Toxicology, 2014

ABSTRACT Kyungheechunggan-tang (KHCGT), a mixture of 9 herbal aqueous extracts, is known in tradi... more ABSTRACT Kyungheechunggan-tang (KHCGT), a mixture of 9 herbal aqueous extracts, is known in traditional Korean hepatoprotective medicine. To investigate the anti-fibrotic effect of KHCGT and its herbal ingredients, hepatic stellate cells (HSC) were activated by transforming growth factor-β (TGF-β), and the mRNA and protein expression levels of collagen type-1 (collagen-1) and α-smooth muscle actin (α-SMA) were measured. KHCGT and its 7 herbal ingredient among 9 herbal ingredients had an anti-fibrotic effect on activated HSC. Among the herbal samples, Artemisia capillaris, Salvia miltiorrhiza, Curcuma wenyujin showed the highest down-regulatory effect on α-SMA and collagen-1 mRNA expression. The liver tissues of dimethylnitrosamine (DMN)-induced rats were isolated to investigate the anti-fibrotic effect of KHCGT. This study provides understandings the effect of KHCGT and its ingredient herbal medicines on the activated HSC, and suggests herbal ingredient that plays a major role in anti-fibrotic effect of the KHCGT.

Scientific Reports

Pancreatic cancer(PC) is less common than other cancers; however, it has a poor prognosis. Theref... more Pancreatic cancer(PC) is less common than other cancers; however, it has a poor prognosis. Therefore, studying novel target signaling and anticancer agents is necessary. Momordicae Semen (MS), the seed of Momordica sochinensis Spreng, mainly found in South-East Asia, including China and Bangladesh, is used to treat various diseases because of its anticancer, antioxidant, anti-inflammatory, and antibacterial properties. However, the effect of the MS extract on pancreatic cancer cells remains unknown. In this study investigated whether the MS extract exerted an anti-cancer effect by regulating c-Myc through CNOT2. Cytotoxicity and proliferation were investigated using MTT and colony formation assays. The levels of apoptotic, oncogenic, and migration-associated factors were confirmed using immunoblotting and immunofluorescence. Wound closure was analyzed using a wound healing assay. The chemical composition of the MS methanol extracts was analyzed using liquid chromatography–mass spect...