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Research paper thumbnail of Comparative proteomic analysis of Salmonella typhimurium LT2 and its hisG gene inactivated mutant

Molecular Genetics, Microbiology and Virology, 2015

ABSTRACT A hisG gene, encoding ATP phosphoribosyl phosphothansferase, insertion mutation was foun... more ABSTRACT A hisG gene, encoding ATP phosphoribosyl phosphothansferase, insertion mutation was found to effected the production of four different proteins in Salmonella Typhimurium LT2 as a results of comparative analysis of two-dimensional analysis of hisG mutant (Δhis87) and the conrol strain (LT2). MALDI-TOF analysis of these protein spots which were expresed in S. Typhimurium LT2 but not its mutant Δhis87, showed that these proteins were; GMP synthase, arginine-tRNA ligase (Arginyl-tRNA synthetase), SopE and SifA. All of these proteins are virulence-associated proteins in Salmonella. Thus we have concluded that the proper function of histidine operon is very crucial for virulence of S. Typhimurium.

Research paper thumbnail of Comparative proteomic analysis of Salmonella typhimurium LT2 and its hisG gene inactivated mutant

Molecular Genetics, Microbiology and Virology, 2015

ABSTRACT A hisG gene, encoding ATP phosphoribosyl phosphothansferase, insertion mutation was foun... more ABSTRACT A hisG gene, encoding ATP phosphoribosyl phosphothansferase, insertion mutation was found to effected the production of four different proteins in Salmonella Typhimurium LT2 as a results of comparative analysis of two-dimensional analysis of hisG mutant (Δhis87) and the conrol strain (LT2). MALDI-TOF analysis of these protein spots which were expresed in S. Typhimurium LT2 but not its mutant Δhis87, showed that these proteins were; GMP synthase, arginine-tRNA ligase (Arginyl-tRNA synthetase), SopE and SifA. All of these proteins are virulence-associated proteins in Salmonella. Thus we have concluded that the proper function of histidine operon is very crucial for virulence of S. Typhimurium.

Research paper thumbnail of Comparative proteomic analysis of Salmonella typhimurium LT2 and its hisG gene inactivated mutant

Molecular Genetics, Microbiology and Virology, 2015

ABSTRACT A hisG gene, encoding ATP phosphoribosyl phosphothansferase, insertion mutation was foun... more ABSTRACT A hisG gene, encoding ATP phosphoribosyl phosphothansferase, insertion mutation was found to effected the production of four different proteins in Salmonella Typhimurium LT2 as a results of comparative analysis of two-dimensional analysis of hisG mutant (Δhis87) and the conrol strain (LT2). MALDI-TOF analysis of these protein spots which were expresed in S. Typhimurium LT2 but not its mutant Δhis87, showed that these proteins were; GMP synthase, arginine-tRNA ligase (Arginyl-tRNA synthetase), SopE and SifA. All of these proteins are virulence-associated proteins in Salmonella. Thus we have concluded that the proper function of histidine operon is very crucial for virulence of S. Typhimurium.

Research paper thumbnail of Comparative proteomic analysis of Salmonella typhimurium LT2 and its hisG gene inactivated mutant

Molecular Genetics, Microbiology and Virology, 2015

ABSTRACT A hisG gene, encoding ATP phosphoribosyl phosphothansferase, insertion mutation was foun... more ABSTRACT A hisG gene, encoding ATP phosphoribosyl phosphothansferase, insertion mutation was found to effected the production of four different proteins in Salmonella Typhimurium LT2 as a results of comparative analysis of two-dimensional analysis of hisG mutant (Δhis87) and the conrol strain (LT2). MALDI-TOF analysis of these protein spots which were expresed in S. Typhimurium LT2 but not its mutant Δhis87, showed that these proteins were; GMP synthase, arginine-tRNA ligase (Arginyl-tRNA synthetase), SopE and SifA. All of these proteins are virulence-associated proteins in Salmonella. Thus we have concluded that the proper function of histidine operon is very crucial for virulence of S. Typhimurium.

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