Ishita Matai - Academia.edu (original) (raw)

Papers by Ishita Matai

Biomedical Applications of Electrospinning and Electrospraying, 2021

Abstract Nanotechnology has made high impact in the field of translational research especially fo... more Abstract Nanotechnology has made high impact in the field of translational research especially for the biomedical applications including drug delivery, tissue engineering, along with nanopharmaceutical product development. Among the nanostructure fabrication methodologies, electrohydrodynamics offers great versatility for the fabrication of nanofibers (NFs) or nanoparticles via electrospinning or electrospraying techniques. Nano-/microparticles and NFs have been widely employed as carriers of choice for delivery of synthetic or bioactive payloads including synthetic drugs, live cells, growth factors, etc. The designed NF/nano-/microparticles could be personalized or tailored for targeted release of their contents due to the flexibility of the methods which led to manifold applications including the tissue engineering scaffolds to medical devices for drug delivery or for theranostic applications. Interestingly, the combination approaches of electrospinning and spraying together have been done recently which hold great promise for biomedical applications. This chapter details about the recent advances in the combination approaches for numerous applications including drug delivery, diagnostics, and antimicrobial applications.

Journal of Electroanalytical Chemistry

ACS Biomaterials Science & Engineering, 2021

An alarming increase in implant failure incidence due to microbial colonization on the administer... more An alarming increase in implant failure incidence due to microbial colonization on the administered orthopedic implants has become a horrifying threat to replacement surgeries and related health concerns. In essence, microbial adhesion and its subsequent biofilm formation, antibiotic resistance, and the host immune system's deficiency are the main culprits. An advanced class of biomaterials termed anti-infective hydrogel implant coatings are evolving to subdue these complications. On this account, this review provides an insight into the significance of anti-infective hydrogels for preventing orthopedic implant associated infections to improve the bone healing process. We briefly discuss the clinical course of implant failure, with a prime focus on orthopedic implants. We identify the different anti-infective coating strategies and hence several anti-infective agents which could be incorporated in the hydrogel matrix. The fundamental design criteria to be considered while fabricating anti-infective hydrogels for orthopedic implants will be discussed. We highlight the different hydrogel coatings based on the origin of the polymers involved in light of their antimicrobial efficacy. We summarize the relevant patents reported in the prevention of implant infections, including orthopedics. Finally, the challenges concerning the clinical translation of the aforesaid hydrogels are described, and considerable solutions for improved clinical practice and better future prospects are proposed.

Microchimica Acta, 2021

A fluorescent nanoprobe based on copper nanoclusters (CuNCs) has been developed for ratiometric d... more A fluorescent nanoprobe based on copper nanoclusters (CuNCs) has been developed for ratiometric detection of hydroxyl radicals ( • OH) and superoxide anion radicals (O 2 •− ). Two differently luminescent CuNCs, namely cyan-emissive poly(methacrylic acid)–protected copper nanoclusters (PCuNCs) and orange-emissive bovine serum albumin–protected CuNCs (BCuNCs), were conjugated to obtain a hybrid, dual-emission nanoprobe (PCuNCs-BCuNCs) with the corresponding peaks at 445 nm and 652 nm at an excitation wavelength of 360 nm. In particular, the fluorescence peak at 445 nm gradually enhanced with the incremental addition of • OH and O 2 •− . However, the fluorescence emission at 652 nm was greatly quenched in the presence of • OH, while in case of O 2 •− , the fluorescence intensity remained constant. The differential response of the PCuNCs-BCuNCs towards • OH and O 2 •− formed the basis of ratiometric detection. Under optimal conditions, the PCuNCs-BCuNCs exhibited good sensitivity and linearity towards • OH and O 2 •− with limits of detection of 0.15 μM and 1.8 μM, respectively. Moreover, the nanoprobe exhibited high selectivity for • OH and O 2 •− over other potential ROS interferences. Besides, PCuNCs-BCuNCs were eventually applied for qualitative and quantitative ratiometric assessment of intracellular • OH and O 2 •− in L-132 cells. Therefore, this strategy unveils a new potential for copper nanocluster–based sensing of ROS. Graphical abstract

ACS Applied Bio Materials

Microchimica Acta, 2021

Peroxynitrite anion (ONOO−) is an important in vivo oxidative stress biomarker whose aberrant lev... more Peroxynitrite anion (ONOO−) is an important in vivo oxidative stress biomarker whose aberrant levels have pathophysiological implications. In this study, an electrochemical sensor for ONOO− detection was developed based on graphene nanoplatelets-cerium oxide nanocomposite (GNP-CeO2) incorporated polyaniline (PANI) conducting hydrogels. The nanocomposite-hydrogel platform exhibited distinct synergistic advantages in terms of large electroactive surface coverage and providing a conductive pathway for electron transfer. Besides, the 3D porous structure of hydrogel integrated the GNP-CeO2 nanocomposite to provide hybrid materials for the evolution of catalytic activity towards electrochemical oxidation of ONOO−. Various microscopic and spectroscopic characterization techniques endorsed the successful formation of GNP-CeO2-PANI hydrogel. Cyclic voltammetry (CV) measurements of GNP-CeO2-PANI hydrogel modified screen-printed electrodes (SPE) were carried out to record the current changes influenced by ONOO−. The prepared sensor demonstrated a significant dose-dependent increase in CV peak current within a linear range of 5–100 µM (at a potential of 1.12 V), and a detection limit of 0.14 with a sensitivity of 29.35 ± 1.4 μA μM−1. Further, a customized microfluidic flow system was integrated with the GNP-CeO2-PANI hydrogel modified SPE to enable continuous electrochemical detection of ONOO− at low sample volumes. The developed microfluidic electrochemical device demonstrated an excellent sensitivity towards ONOO− under optimal experimental conditions. Overall, the fabricated microfluidic device with hybrid hydrogels as electrochemical interfaces provides a reliable assessment of ONOO− levels. This work offers considerable potential for understanding the oxidative stress–related disease mechanisms through determination of ONOO− in biological samples.

Unprecedented advances in nanoparticle research for a host of therapeutic purposes, directly or a... more Unprecedented advances in nanoparticle research for a host of therapeutic purposes, directly or as delivery agents have revolutionized the field of medical science. The most significant aspect is to develop the disease and target-specific nanoparticles with improved efficacy, with minimal side effects. Nanopharmacology is a holistic branch of science involving the amalgamation of chemistry, engineering, biology, and medicine, and evaluates the interactions of nanoparticles with the living systems to render the creation of safer and efficient formulations. Over the last decade, nanoparticle-based delivery modalities such as peptides, carbon nanotubes, nanodiamonds, liposomes, quantum dots, graphene, and metal-based nanoparticles have shown huge therapeutic potential by enhancing the pharmacokinetics of the drug and site-specific delivery. The main aim of developing these nanostructures is to understand the disease pathology and thereby enhance the therapeutic index of patients. There...

3D Printing Technology in Nanomedicine, 2019

Progress in Materials Science, 2021

Analytical and Bioanalytical Chemistry, 2020

Despite recent advancements in the field of microfluidic paper-based analytical devices (μPADs), ... more Despite recent advancements in the field of microfluidic paper-based analytical devices (μPADs), a key challenge remains in developing a simple and efficient μPAD with customized imaging capabilities for antioxidant assays. In the present study, we report a facile approach for μPAD fabrication through the application of transparent nail paint leading to creation of hydrophobic barriers and well-defined channels. The resultant μPADs were then characterized through scanning electron microscopy and contact angle measurements. The resolution and functional features of the fabricated μPAD were amenable to the intended assay. The μPAD's impregnated poly(methacrylic acid) (PMAA)-coated cerium oxide (CeO2) nanoparticles oxidized the 3,3′,5,5′-tetramethylbenzidine (TMB) leading to the formation of a blue-colored charge-transfer complex. The addition of different antioxidant standard solutions resulted in a reduction in the blue color in a dose-dependent manner which could be observed visually. The color intensity of the PMAA-CeO2 nanoparticle@TMB oxidation product was inversely proportional to the antioxidant concentration and was measured using customized in-house MATLAB-based image processing software. Importantly, PMAA-CeO2 nanoparticle-based μPADs demonstrated good analytical characteristics and were able to be stored for long periods without any loss of activity. Moreover, potential interferents did not pose any threat to the colorimetric signal read-out for determination of antioxidant activity. The developed method was further applied for the assessment of antioxidant activity in a variety of tea samples and performed satisfactorily in comparison with a commonly used antioxidant detection method. Collectively, the developed μPAD-based platform holds great potential as a low-cost, convenient, portable and reliable method for pursuing various on-site antioxidant assays. Graphical Abstract Graphical Abstract

New Journal of Chemistry, 2020

A new dimension for the selective detection of short-lived ROS by an electroactive reduced graphe... more A new dimension for the selective detection of short-lived ROS by an electroactive reduced graphene oxide–cerium oxide nanocomposite@cytochrome c hydrogel.

Journal of Photochemistry and Photobiology B: Biology, 2020

Nanotechnology driven cancer theranostics hold potential as promising future clinical modalities.... more Nanotechnology driven cancer theranostics hold potential as promising future clinical modalities. Currently, there is a strong emphasis on the development of combinational modalities, especially for cancer treatment. In this study, we present a topical hydrogel patch for nanomaterial-assisted photothermal therapeutics as well as for on-demand drug delivery application. The patch was derived from interpenetrating networks (IPNs) of alginate (Alg) and polyacrylamide (PAAm) in weight ratio 8:1 by free radical polymerization. The patch interiors were composed of hybrid nanostructures derived from gold nanorods (AuNRs) anchored onto polyvinylpyrrolidone (PVP) functionalized graphene oxide (PVP-nGO) to form PVP-nGO@AuNRs hybrids. Field emission scanning electron microscopy (FE-SEM) images revealed the porous nature of the hybrid hydrogel patch with an average pore size of ~28.60 ± 3.10 μm. Besides, functional characteristics of the hybrid patch, such as mechanical strength, viscoelastic and swelling behavior, were investigated. Under near-infrared (NIR) radiation exposure, the hybrid patch exhibited photothermal properties such as surface temperature rise to 75.16 ± 0.32 °C, sufficient to ablate cancer cells thermally. Besides, the heat generated in the hybrid patch could be transmitted to an underlying hydrogel (mimicking skin tissue) when stacked together without much loss. Under cyclic photothermal heating, the patch could retain its photothermal stability for four cycles. Furthermore, the hybrid patch demonstrated NIR stimulated drug release, which was evaluated using methotrexate (MTX, water-insoluble anticancer drug) and rhodamine B (RhB, water-soluble dye). Taken together, this work provides a new dimension towards the development of externally placed hydrogel patches for thermal destruction of localized solid tumors and tunable delivery of chemotherapeutic drugs at the target site.

Nano Express, 2020

The exploitation of plant extracts for the synthesis of nano selenium having antibacterial and an... more The exploitation of plant extracts for the synthesis of nano selenium having antibacterial and antioxidant activities is an exciting approach to counteract the prevalence of infections caused by antibiotic-resistant bacteria, which holds relevance for medical and food industries. In the present work, a green and facile method for the preparation of nano selenium (nSe) using the fruit extract of Indian gooseberry (Phyllanthus Emblica) has been reported. The optical and structural properties of the as-synthesized nSe were studied through various characterization techniques. Eventually, the antioxidant potential of nSe was investigated via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl free radical scavenging assays. Parallely, the antibacterial activity of nSe against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa was evaluated. The antioxidant assays indicated that even low dosage of nSe showed excellent activity with EC50 values of 0.21 μg ml−1 and 3.34 μg ml−...

Biomaterials, 2019

Escalating cases of organ shortage and donor scarcity worldwide are alarming reminders of the nee... more Escalating cases of organ shortage and donor scarcity worldwide are alarming reminders of the need for alternatives to allograft tissues. Within the last three decades, research efforts in the field of regenerative medicine and tissue engineering continue to address the unmet need for artificial tissues and organs for transplant. Work in the field has evolved to create what we consider a new field, Regenerative Engineering, defined as the Convergence of advanced materials science, stem cell science, physics, developmental biology and clinical translation towards the regeneration of complex tissues and organ systems. Included in the regenerative engineering paradigm is advanced manufacturing. Three-dimensional (3D) bioprinting is a promising and innovative biofabrication strategy to precisely position biologics, including living cells and extracellular matrix (ECM) components, in the prescribed 3D hierarchal organization to create artificial multi-cellular tissues/organs. In this review, we outline recent progress in several bioprinting technologies used to engineer scaffolds with requisite mechanical, structural, and biological complexity. We examine the process parameters affecting bioprinting and bioink-biomaterials and review notable studies on bioprinted skin, cardiac, bone, cartilage, liver, lung, neural, and pancreatic tissue. We also focus on other 3D bioprinting application areas including cancer research, drug testing, high-throughput screening (HTS), and organ-on-a-chip models. We also highlight the current challenges associated with the clinical translation of 3D bioprinting and conclude with the future perspective of bioprinting technology.

RSC Advances, 2019

Surface functionalization with polydopamine augments the structural stability and antibacterial p... more Surface functionalization with polydopamine augments the structural stability and antibacterial profile of magnetic hydrogel beads.

Analytical Methods, 2019

A facile approach for the fluorescence based detection of sulfite (SO32-) using gold nanoclusters... more A facile approach for the fluorescence based detection of sulfite (SO32-) using gold nanoclusters (Au NCs) has been reported. The detection assay relies on the effective quenching of the fluorescence...

Journal of Polymer Science Part B: Polymer Physics, 2018

Biomedical Applications of Electrospinning and Electrospraying, 2021

Abstract Nanotechnology has made high impact in the field of translational research especially fo... more Abstract Nanotechnology has made high impact in the field of translational research especially for the biomedical applications including drug delivery, tissue engineering, along with nanopharmaceutical product development. Among the nanostructure fabrication methodologies, electrohydrodynamics offers great versatility for the fabrication of nanofibers (NFs) or nanoparticles via electrospinning or electrospraying techniques. Nano-/microparticles and NFs have been widely employed as carriers of choice for delivery of synthetic or bioactive payloads including synthetic drugs, live cells, growth factors, etc. The designed NF/nano-/microparticles could be personalized or tailored for targeted release of their contents due to the flexibility of the methods which led to manifold applications including the tissue engineering scaffolds to medical devices for drug delivery or for theranostic applications. Interestingly, the combination approaches of electrospinning and spraying together have been done recently which hold great promise for biomedical applications. This chapter details about the recent advances in the combination approaches for numerous applications including drug delivery, diagnostics, and antimicrobial applications.

Journal of Electroanalytical Chemistry

ACS Biomaterials Science & Engineering, 2021

An alarming increase in implant failure incidence due to microbial colonization on the administer... more An alarming increase in implant failure incidence due to microbial colonization on the administered orthopedic implants has become a horrifying threat to replacement surgeries and related health concerns. In essence, microbial adhesion and its subsequent biofilm formation, antibiotic resistance, and the host immune system's deficiency are the main culprits. An advanced class of biomaterials termed anti-infective hydrogel implant coatings are evolving to subdue these complications. On this account, this review provides an insight into the significance of anti-infective hydrogels for preventing orthopedic implant associated infections to improve the bone healing process. We briefly discuss the clinical course of implant failure, with a prime focus on orthopedic implants. We identify the different anti-infective coating strategies and hence several anti-infective agents which could be incorporated in the hydrogel matrix. The fundamental design criteria to be considered while fabricating anti-infective hydrogels for orthopedic implants will be discussed. We highlight the different hydrogel coatings based on the origin of the polymers involved in light of their antimicrobial efficacy. We summarize the relevant patents reported in the prevention of implant infections, including orthopedics. Finally, the challenges concerning the clinical translation of the aforesaid hydrogels are described, and considerable solutions for improved clinical practice and better future prospects are proposed.

Microchimica Acta, 2021

A fluorescent nanoprobe based on copper nanoclusters (CuNCs) has been developed for ratiometric d... more A fluorescent nanoprobe based on copper nanoclusters (CuNCs) has been developed for ratiometric detection of hydroxyl radicals ( • OH) and superoxide anion radicals (O 2 •− ). Two differently luminescent CuNCs, namely cyan-emissive poly(methacrylic acid)–protected copper nanoclusters (PCuNCs) and orange-emissive bovine serum albumin–protected CuNCs (BCuNCs), were conjugated to obtain a hybrid, dual-emission nanoprobe (PCuNCs-BCuNCs) with the corresponding peaks at 445 nm and 652 nm at an excitation wavelength of 360 nm. In particular, the fluorescence peak at 445 nm gradually enhanced with the incremental addition of • OH and O 2 •− . However, the fluorescence emission at 652 nm was greatly quenched in the presence of • OH, while in case of O 2 •− , the fluorescence intensity remained constant. The differential response of the PCuNCs-BCuNCs towards • OH and O 2 •− formed the basis of ratiometric detection. Under optimal conditions, the PCuNCs-BCuNCs exhibited good sensitivity and linearity towards • OH and O 2 •− with limits of detection of 0.15 μM and 1.8 μM, respectively. Moreover, the nanoprobe exhibited high selectivity for • OH and O 2 •− over other potential ROS interferences. Besides, PCuNCs-BCuNCs were eventually applied for qualitative and quantitative ratiometric assessment of intracellular • OH and O 2 •− in L-132 cells. Therefore, this strategy unveils a new potential for copper nanocluster–based sensing of ROS. Graphical abstract

ACS Applied Bio Materials

Microchimica Acta, 2021

Peroxynitrite anion (ONOO−) is an important in vivo oxidative stress biomarker whose aberrant lev... more Peroxynitrite anion (ONOO−) is an important in vivo oxidative stress biomarker whose aberrant levels have pathophysiological implications. In this study, an electrochemical sensor for ONOO− detection was developed based on graphene nanoplatelets-cerium oxide nanocomposite (GNP-CeO2) incorporated polyaniline (PANI) conducting hydrogels. The nanocomposite-hydrogel platform exhibited distinct synergistic advantages in terms of large electroactive surface coverage and providing a conductive pathway for electron transfer. Besides, the 3D porous structure of hydrogel integrated the GNP-CeO2 nanocomposite to provide hybrid materials for the evolution of catalytic activity towards electrochemical oxidation of ONOO−. Various microscopic and spectroscopic characterization techniques endorsed the successful formation of GNP-CeO2-PANI hydrogel. Cyclic voltammetry (CV) measurements of GNP-CeO2-PANI hydrogel modified screen-printed electrodes (SPE) were carried out to record the current changes influenced by ONOO−. The prepared sensor demonstrated a significant dose-dependent increase in CV peak current within a linear range of 5–100 µM (at a potential of 1.12 V), and a detection limit of 0.14 with a sensitivity of 29.35 ± 1.4 μA μM−1. Further, a customized microfluidic flow system was integrated with the GNP-CeO2-PANI hydrogel modified SPE to enable continuous electrochemical detection of ONOO− at low sample volumes. The developed microfluidic electrochemical device demonstrated an excellent sensitivity towards ONOO− under optimal experimental conditions. Overall, the fabricated microfluidic device with hybrid hydrogels as electrochemical interfaces provides a reliable assessment of ONOO− levels. This work offers considerable potential for understanding the oxidative stress–related disease mechanisms through determination of ONOO− in biological samples.

Unprecedented advances in nanoparticle research for a host of therapeutic purposes, directly or a... more Unprecedented advances in nanoparticle research for a host of therapeutic purposes, directly or as delivery agents have revolutionized the field of medical science. The most significant aspect is to develop the disease and target-specific nanoparticles with improved efficacy, with minimal side effects. Nanopharmacology is a holistic branch of science involving the amalgamation of chemistry, engineering, biology, and medicine, and evaluates the interactions of nanoparticles with the living systems to render the creation of safer and efficient formulations. Over the last decade, nanoparticle-based delivery modalities such as peptides, carbon nanotubes, nanodiamonds, liposomes, quantum dots, graphene, and metal-based nanoparticles have shown huge therapeutic potential by enhancing the pharmacokinetics of the drug and site-specific delivery. The main aim of developing these nanostructures is to understand the disease pathology and thereby enhance the therapeutic index of patients. There...

3D Printing Technology in Nanomedicine, 2019

Progress in Materials Science, 2021

Analytical and Bioanalytical Chemistry, 2020

Despite recent advancements in the field of microfluidic paper-based analytical devices (μPADs), ... more Despite recent advancements in the field of microfluidic paper-based analytical devices (μPADs), a key challenge remains in developing a simple and efficient μPAD with customized imaging capabilities for antioxidant assays. In the present study, we report a facile approach for μPAD fabrication through the application of transparent nail paint leading to creation of hydrophobic barriers and well-defined channels. The resultant μPADs were then characterized through scanning electron microscopy and contact angle measurements. The resolution and functional features of the fabricated μPAD were amenable to the intended assay. The μPAD's impregnated poly(methacrylic acid) (PMAA)-coated cerium oxide (CeO2) nanoparticles oxidized the 3,3′,5,5′-tetramethylbenzidine (TMB) leading to the formation of a blue-colored charge-transfer complex. The addition of different antioxidant standard solutions resulted in a reduction in the blue color in a dose-dependent manner which could be observed visually. The color intensity of the PMAA-CeO2 nanoparticle@TMB oxidation product was inversely proportional to the antioxidant concentration and was measured using customized in-house MATLAB-based image processing software. Importantly, PMAA-CeO2 nanoparticle-based μPADs demonstrated good analytical characteristics and were able to be stored for long periods without any loss of activity. Moreover, potential interferents did not pose any threat to the colorimetric signal read-out for determination of antioxidant activity. The developed method was further applied for the assessment of antioxidant activity in a variety of tea samples and performed satisfactorily in comparison with a commonly used antioxidant detection method. Collectively, the developed μPAD-based platform holds great potential as a low-cost, convenient, portable and reliable method for pursuing various on-site antioxidant assays. Graphical Abstract Graphical Abstract

New Journal of Chemistry, 2020

A new dimension for the selective detection of short-lived ROS by an electroactive reduced graphe... more A new dimension for the selective detection of short-lived ROS by an electroactive reduced graphene oxide–cerium oxide nanocomposite@cytochrome c hydrogel.

Journal of Photochemistry and Photobiology B: Biology, 2020

Nanotechnology driven cancer theranostics hold potential as promising future clinical modalities.... more Nanotechnology driven cancer theranostics hold potential as promising future clinical modalities. Currently, there is a strong emphasis on the development of combinational modalities, especially for cancer treatment. In this study, we present a topical hydrogel patch for nanomaterial-assisted photothermal therapeutics as well as for on-demand drug delivery application. The patch was derived from interpenetrating networks (IPNs) of alginate (Alg) and polyacrylamide (PAAm) in weight ratio 8:1 by free radical polymerization. The patch interiors were composed of hybrid nanostructures derived from gold nanorods (AuNRs) anchored onto polyvinylpyrrolidone (PVP) functionalized graphene oxide (PVP-nGO) to form PVP-nGO@AuNRs hybrids. Field emission scanning electron microscopy (FE-SEM) images revealed the porous nature of the hybrid hydrogel patch with an average pore size of ~28.60 ± 3.10 μm. Besides, functional characteristics of the hybrid patch, such as mechanical strength, viscoelastic and swelling behavior, were investigated. Under near-infrared (NIR) radiation exposure, the hybrid patch exhibited photothermal properties such as surface temperature rise to 75.16 ± 0.32 °C, sufficient to ablate cancer cells thermally. Besides, the heat generated in the hybrid patch could be transmitted to an underlying hydrogel (mimicking skin tissue) when stacked together without much loss. Under cyclic photothermal heating, the patch could retain its photothermal stability for four cycles. Furthermore, the hybrid patch demonstrated NIR stimulated drug release, which was evaluated using methotrexate (MTX, water-insoluble anticancer drug) and rhodamine B (RhB, water-soluble dye). Taken together, this work provides a new dimension towards the development of externally placed hydrogel patches for thermal destruction of localized solid tumors and tunable delivery of chemotherapeutic drugs at the target site.

Nano Express, 2020

The exploitation of plant extracts for the synthesis of nano selenium having antibacterial and an... more The exploitation of plant extracts for the synthesis of nano selenium having antibacterial and antioxidant activities is an exciting approach to counteract the prevalence of infections caused by antibiotic-resistant bacteria, which holds relevance for medical and food industries. In the present work, a green and facile method for the preparation of nano selenium (nSe) using the fruit extract of Indian gooseberry (Phyllanthus Emblica) has been reported. The optical and structural properties of the as-synthesized nSe were studied through various characterization techniques. Eventually, the antioxidant potential of nSe was investigated via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl free radical scavenging assays. Parallely, the antibacterial activity of nSe against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa was evaluated. The antioxidant assays indicated that even low dosage of nSe showed excellent activity with EC50 values of 0.21 μg ml−1 and 3.34 μg ml−...

Biomaterials, 2019

Escalating cases of organ shortage and donor scarcity worldwide are alarming reminders of the nee... more Escalating cases of organ shortage and donor scarcity worldwide are alarming reminders of the need for alternatives to allograft tissues. Within the last three decades, research efforts in the field of regenerative medicine and tissue engineering continue to address the unmet need for artificial tissues and organs for transplant. Work in the field has evolved to create what we consider a new field, Regenerative Engineering, defined as the Convergence of advanced materials science, stem cell science, physics, developmental biology and clinical translation towards the regeneration of complex tissues and organ systems. Included in the regenerative engineering paradigm is advanced manufacturing. Three-dimensional (3D) bioprinting is a promising and innovative biofabrication strategy to precisely position biologics, including living cells and extracellular matrix (ECM) components, in the prescribed 3D hierarchal organization to create artificial multi-cellular tissues/organs. In this review, we outline recent progress in several bioprinting technologies used to engineer scaffolds with requisite mechanical, structural, and biological complexity. We examine the process parameters affecting bioprinting and bioink-biomaterials and review notable studies on bioprinted skin, cardiac, bone, cartilage, liver, lung, neural, and pancreatic tissue. We also focus on other 3D bioprinting application areas including cancer research, drug testing, high-throughput screening (HTS), and organ-on-a-chip models. We also highlight the current challenges associated with the clinical translation of 3D bioprinting and conclude with the future perspective of bioprinting technology.

RSC Advances, 2019

Surface functionalization with polydopamine augments the structural stability and antibacterial p... more Surface functionalization with polydopamine augments the structural stability and antibacterial profile of magnetic hydrogel beads.

Analytical Methods, 2019

A facile approach for the fluorescence based detection of sulfite (SO32-) using gold nanoclusters... more A facile approach for the fluorescence based detection of sulfite (SO32-) using gold nanoclusters (Au NCs) has been reported. The detection assay relies on the effective quenching of the fluorescence...

Journal of Polymer Science Part B: Polymer Physics, 2018