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Papers by Isabelle Sylvestre
European Journal of Organic Chemistry, 2004
Four 14-membered azamacrocycles 2a−d, each containing a pyridine moiety and three acetic and/or p... more Four 14-membered azamacrocycles 2a−d, each containing a pyridine moiety and three acetic and/or propionic acid pendent arms, were prepared from regioselectively functionalised polyamines. The stability constants of the lanthanide complexes (La, Pr, Nd, Eu, Gd, Tb, Er, Yb, Lu) formed with the triacetic acid compound 2a were determined by spectroscopic methods (luminescence and/or UV/Vis spectroscopy). Other characteristics of the luminescent EuIII and TbIII complexes formed with the triacetic acid derivative 2a were also evaluated. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004)
Cheminform, 2010
ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was e... more ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Scientia Horticulturae, 2011
In this study, in vitro shoot tips of two sugarcane clones were successfully cryopreserved using ... more In this study, in vitro shoot tips of two sugarcane clones were successfully cryopreserved using encapsulation-dehydration and droplet-vitrification with two vitrification solutions, PVS2 and PVS3. For both clones, encapsulation-dehydration induced significantly higher recovery, reaching 60% for clone H70-144 and 53% for clone CP68-1026, compared with droplet-vitrification in which recovery was 33–37% for clone H70-144 and 20–27% for clone CP68-1026. Optimal
Scientia Horticulturae, 2011
Apical shoot tips excised from in vitro plantlets of blackberry (Rubus fruticosus L. 'Čačanska Be... more Apical shoot tips excised from in vitro plantlets of blackberry (Rubus fruticosus L. 'Čačanska Bestrna') and cherry plum (Prunus cerasifera Ehrh.) were tested for recovery after cryopreservation using the dropletvitrification technique. Following treatment for 30 min with a loading solution comprising 1.9 M glycerol and 0.5 M sucrose, explants were dehydrated with a highly concentrated cryoprotectant solution, so called vitrification solution. Shoot tips were dehydrated for 10, 20 and 30 min at room temperature with a solution derived from the original PVS2 solution (containing 37.5% (w/v) glycerol, 15% (w/v) dimethylsulfoxide, 15% (w/v) ethylene glycol and 22.5% (w/v) sucrose) and for 60, 90 and 120 min using the PVS3 solution (containing 50% (w/v) glycerol and 50% (w/v) sucrose). Explants were cooled by direct immersion in LN in 10 l droplets of vitrification solution placed on aluminium foil strips. Rewarming was done by direct plunging of foil strips in a preheated (37 • C) unloading solution (0.8 M sucrose) for 30 s, after which an equal volume of unloading solution (at room temperature) was added for further incubation for 30 min. As for regrowth of blackberry, PVS3 proved more effective than the modified PVS2, but the difference was significant (P < 0.05) only for the shortest treatment duration. The duration of PVS3 treatment had no significant effect on regrowth of cryopreserved shoot tips (45.8-70%). By contrast, a 30-min treatment with modified PVS2 solution resulted in a significant increase in regeneration percentage (30%), as compared with a 10-min treatment with the same solution (5%). Cherry plum shoot tips were very sensitive to both vitrification solutions and growth recovery of cryopreserved samples was generally lower (5-20%) than that of blackberry explants. No significant influence of PVS treatment (both type of solution and treatment duration) on regrowth of cryopreserved shoot tips was observed with cherry plum shoot tips. Experiments performed in France and in Serbia produced similar results, thereby showing the robustness and reproducibility of the protocols developed.
Scientia Horticulturae, 2011
In this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully ... more In this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully cryopreserved using the droplet-vitrification technique. Growth recovery of cryopreserved shoot tips was possible only when samples were pretreated for 16 h in liquid medium with 0.3 M sucrose, then for 5 h in liquid medium with 0.7 M sucrose before performing the cryopreservation protocol. Optimal conditions included treatment for 20 min in a loading solution containing 1.9 M glycerol + 0.5 M sucrose, treatment with vitrification solution B5 (glycerol 40.0%, sucrose 40.0%, w/v) for 60 and 90 min or vitrification solution A9 (glycerol 30.0%, dimethylsulfoxide 20.0%, ethylene glycol 20.0%, sucrose 15.0%) for 20 min, rapid cooling in minute droplets of vitrification solution, rapid rewarming by immersion for 20 min in unloading solution containing 1.2 M sucrose. Under these conditions, 37% recovery of cryopreserved shoot tips was achieved. Regrowth of cryopreserved samples was slow but always direct, without callus formation.
European Journal of Organic Chemistry, 2002
cyclopropane derivatives Q 0021
Cheminform, 2010
For Abstract see ChemInform Abstract in Full Text.
Tetrahedron Letters, 2001
The titanium(IV)-mediated cyclopropanation of ethyl b-chloropropionate by Grignard reagents and i... more The titanium(IV)-mediated cyclopropanation of ethyl b-chloropropionate by Grignard reagents and in situ tosylation, followed by base-induced dehydrochlorination provided diastereoselectivity pure (E)-2-alkyl-1-ethenyl-1-(tosyloxy)cyclopropanes, suitable precursors of 1,1-ethyleneallylmetal species of significant synthetic potential.
Cheminform, 2010
ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was e... more ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
European Journal of Organic Chemistry, 2004
Four 14-membered azamacrocycles 2a−d, each containing a pyridine moiety and three acetic and/or p... more Four 14-membered azamacrocycles 2a−d, each containing a pyridine moiety and three acetic and/or propionic acid pendent arms, were prepared from regioselectively functionalised polyamines. The stability constants of the lanthanide complexes (La, Pr, Nd, Eu, Gd, Tb, Er, Yb, Lu) formed with the triacetic acid compound 2a were determined by spectroscopic methods (luminescence and/or UV/Vis spectroscopy). Other characteristics of the luminescent EuIII and TbIII complexes formed with the triacetic acid derivative 2a were also evaluated. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004)
Cheminform, 2010
ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was e... more ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Scientia Horticulturae, 2011
In this study, in vitro shoot tips of two sugarcane clones were successfully cryopreserved using ... more In this study, in vitro shoot tips of two sugarcane clones were successfully cryopreserved using encapsulation-dehydration and droplet-vitrification with two vitrification solutions, PVS2 and PVS3. For both clones, encapsulation-dehydration induced significantly higher recovery, reaching 60% for clone H70-144 and 53% for clone CP68-1026, compared with droplet-vitrification in which recovery was 33–37% for clone H70-144 and 20–27% for clone CP68-1026. Optimal
Scientia Horticulturae, 2011
Apical shoot tips excised from in vitro plantlets of blackberry (Rubus fruticosus L. 'Čačanska Be... more Apical shoot tips excised from in vitro plantlets of blackberry (Rubus fruticosus L. 'Čačanska Bestrna') and cherry plum (Prunus cerasifera Ehrh.) were tested for recovery after cryopreservation using the dropletvitrification technique. Following treatment for 30 min with a loading solution comprising 1.9 M glycerol and 0.5 M sucrose, explants were dehydrated with a highly concentrated cryoprotectant solution, so called vitrification solution. Shoot tips were dehydrated for 10, 20 and 30 min at room temperature with a solution derived from the original PVS2 solution (containing 37.5% (w/v) glycerol, 15% (w/v) dimethylsulfoxide, 15% (w/v) ethylene glycol and 22.5% (w/v) sucrose) and for 60, 90 and 120 min using the PVS3 solution (containing 50% (w/v) glycerol and 50% (w/v) sucrose). Explants were cooled by direct immersion in LN in 10 l droplets of vitrification solution placed on aluminium foil strips. Rewarming was done by direct plunging of foil strips in a preheated (37 • C) unloading solution (0.8 M sucrose) for 30 s, after which an equal volume of unloading solution (at room temperature) was added for further incubation for 30 min. As for regrowth of blackberry, PVS3 proved more effective than the modified PVS2, but the difference was significant (P < 0.05) only for the shortest treatment duration. The duration of PVS3 treatment had no significant effect on regrowth of cryopreserved shoot tips (45.8-70%). By contrast, a 30-min treatment with modified PVS2 solution resulted in a significant increase in regeneration percentage (30%), as compared with a 10-min treatment with the same solution (5%). Cherry plum shoot tips were very sensitive to both vitrification solutions and growth recovery of cryopreserved samples was generally lower (5-20%) than that of blackberry explants. No significant influence of PVS treatment (both type of solution and treatment duration) on regrowth of cryopreserved shoot tips was observed with cherry plum shoot tips. Experiments performed in France and in Serbia produced similar results, thereby showing the robustness and reproducibility of the protocols developed.
Scientia Horticulturae, 2011
In this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully ... more In this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully cryopreserved using the droplet-vitrification technique. Growth recovery of cryopreserved shoot tips was possible only when samples were pretreated for 16 h in liquid medium with 0.3 M sucrose, then for 5 h in liquid medium with 0.7 M sucrose before performing the cryopreservation protocol. Optimal conditions included treatment for 20 min in a loading solution containing 1.9 M glycerol + 0.5 M sucrose, treatment with vitrification solution B5 (glycerol 40.0%, sucrose 40.0%, w/v) for 60 and 90 min or vitrification solution A9 (glycerol 30.0%, dimethylsulfoxide 20.0%, ethylene glycol 20.0%, sucrose 15.0%) for 20 min, rapid cooling in minute droplets of vitrification solution, rapid rewarming by immersion for 20 min in unloading solution containing 1.2 M sucrose. Under these conditions, 37% recovery of cryopreserved shoot tips was achieved. Regrowth of cryopreserved samples was slow but always direct, without callus formation.
European Journal of Organic Chemistry, 2002
cyclopropane derivatives Q 0021
Cheminform, 2010
For Abstract see ChemInform Abstract in Full Text.
Tetrahedron Letters, 2001
The titanium(IV)-mediated cyclopropanation of ethyl b-chloropropionate by Grignard reagents and i... more The titanium(IV)-mediated cyclopropanation of ethyl b-chloropropionate by Grignard reagents and in situ tosylation, followed by base-induced dehydrochlorination provided diastereoselectivity pure (E)-2-alkyl-1-ethenyl-1-(tosyloxy)cyclopropanes, suitable precursors of 1,1-ethyleneallylmetal species of significant synthetic potential.
Cheminform, 2010
ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was e... more ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.