Ida Gabriele - Academia.edu (original) (raw)

Papers by Ida Gabriele

International Journal of Immunopathology and Pharmacology, 2011

The avoidance of food(s) is the main therapeutic approach to food allergy. Nevertheless, orally- ... more The avoidance of food(s) is the main therapeutic approach to food allergy. Nevertheless, orally- or sublingually-administered food allergens have gained attention and a number of food-allergic children can tolerate gradually increasing amounts of cow's milk and hen's egg. Our purpose is to show that oral desensitisation with food is an allergen-specific therapeutic approach and for this, we describe 4 illustrative children with IgE-mediated food allergy. The first was allergic to cow's milk and hen's egg, the second to cow's milk, hen's egg and fish. Both underwent oral desensitisation to both cow's milk and hen's egg. The third child was allergic to cow's milk, hen's egg and fish and underwent oral desensitisation with cow's milk. The last child was allergic to raw but not to cooked/boiled hen's egg and underwent the oral desensitisation with hen's egg. The first 2 children reached the clinical tolerance to cow's milk after th...

International Journal of Immunopathology and Pharmacology, 2011

Vγ9Vδ2 T lymphocytes have been shown to respond to a variety of non-peptide antigens including al... more Vγ9Vδ2 T lymphocytes have been shown to respond to a variety of non-peptide antigens including alkylamines and phosphoantigens. Recently, aminobisphosphonates have also been shown to stimulate this subset of γδ+ T cells. In this study we analyzed the proliferative responses of freshly isolated γδ T lymphocytes obtained from human cord blood when challenged with pyrophosphomonoesters or aminobisphosphonates. Nitrogen-containing aminobisphopsphonates, in contrast to phoshoantigens, readily stimulated expansion of Vδ2Vγ9 cells in human cord blood. Expanded cells displayed an activated mature phenotype, and were capable of producing TNFα and IFNγ but not perforin following secondary stimulation, consistent with the development of a regulatory, as opposed to cytotoxic, phenotype. This approach may provide a useful strategy for a new approach to the treatment of neonatal pathologies.

In newborn the innate immune system provides essential protection during primary infections befor... more In newborn the innate immune system provides essential protection during primary infections before the generation of an appropriate adaptive immune response that is initially not fully operative. Innate immune response is evoked and perpetuated by molecules derived from microorganisms or by the damage/death of host cells. These are collectively known as damage-associated molecular-pattern (DAMP) molecules. High-mobility group box 1 protein (HMGB1) or amphoterin, which previously was considered to be only a nuclear factor, has been recently identified as a DAMP molecule. When it is actively secreted by inflammatory cells or passively released from necrotic cells, HMGB1 mediates the response to infection, injury and inflammation, inducing dendritic cells maturation and T helper-1-cell responses. To characterize the role of HMGB1 in the innate and immature defense mechanisms in newborns, human cord blood (CB) mononuclear cells, in comparison to adult peripheral blood (PB) mononuclear c...

The mean neonatal and maternal serum level of anti-tetanus IgG was 3.04 ± 2.47 IU/mL and 3.4 ± 2.... more The mean neonatal and maternal serum level of anti-tetanus IgG was 3.04 ± 2.47 IU/mL and 3.4 ± 2.62 IU/mL, respectively. These levels were not statistically significant. A highly significant correlation was observed between maternal and neonatal anti-tetanus IgG (r = 0.88), as the rate of antitoxin of the infants increased by each 1 IU/mL of the mothers. In total, 95.4% of mothers and 97.7% newborns had protective level (> 0.1 IU/mL) for anti-tetanus IgG. The mean neonatal/maternal blood ratio of anti-tetanus IgG was 0.94 ± 0.57 (0.17-4.33).

Pediatric Allergy and Immunology, 2012

To cite this article: Meglio P, Giampietro PG, Carello R, Gabriele I, Avitabile S, Galli E. Oral ... more To cite this article: Meglio P, Giampietro PG, Carello R, Gabriele I, Avitabile S, Galli E. Oral food desensitization in children with IgE-mediated hen's egg allergy:

Early Human Development, 2008

The mean neonatal and maternal serum level of anti-tetanus IgG was 3.04 ± 2.47 IU/mL and 3.4 ± 2.... more The mean neonatal and maternal serum level of anti-tetanus IgG was 3.04 ± 2.47 IU/mL and 3.4 ± 2.62 IU/mL, respectively. These levels were not statistically significant. A highly significant correlation was observed between maternal and neonatal anti-tetanus IgG (r = 0.88), as the rate of antitoxin of the infants increased by each 1 IU/mL of the mothers. In total, 95.4% of mothers and 97.7% newborns had protective level (> 0.1 IU/mL) for anti-tetanus IgG. The mean neonatal/maternal blood ratio of anti-tetanus IgG was 0.94 ± 0.57 (0.17-4.33).

PLoS ONE, 2011

In newborn the innate immune system provides essential protection during primary infections befor... more In newborn the innate immune system provides essential protection during primary infections before the generation of an appropriate adaptive immune response that is initially not fully operative. Innate immune response is evoked and perpetuated by molecules derived from microorganisms or by the damage/death of host cells. These are collectively known as damage-associated molecular-pattern (DAMP) molecules. High-mobility group box 1 protein (HMGB1) or amphoterin, which previously was considered to be only a nuclear factor, has been recently identified as a DAMP molecule. When it is actively secreted by inflammatory cells or passively released from necrotic cells, HMGB1 mediates the response to infection, injury and inflammation, inducing dendritic cells maturation and T helper-1-cell responses. To characterize the role of HMGB1 in the innate and immature defense mechanisms in newborns, human cord blood (CB) mononuclear cells, in comparison to adult peripheral blood (PB) mononuclear cells, have been analyzed for its expression. By flow cytometry and western blot analysis, we observed that in CB and PB cells: i) HMGB1 is expressed on cell surface membranes of myeloid dendritic cell precursors, mostly, and lymphocytes (gamma/delta and CD4(+) T cells) to a lesser extent; ii) different pro-inflammatory stimuli or molecules that mimic infection increased cell surface expression of HMGB1 as well as its secretion into extracellular environment; iii) the treatment with synthetic molecules such as aminobisphosphonates (ABs), identified to be γδ T cell antigens, triggered up-regulation of HMGB1 expression on mononuclear cells, as well γδ T lymphocytes, inducing its secretion. The modulation of its secretion and the HMGB1-mediated migration of monocytes indicated HMGB1 as regulator of immune response in an immature system, like CB, through engagement of γδ T lymphocytes and myeloid dendritic cell precursors, essential components of innate immunity. In addition, the increased HMGB1 expression/secretion triggered by ABs, previously characterized for their immuno-modulating and immune-adjuvant capabilities, indicated that immunomodulation might represent a new therapeutical approach for neonatal and adult pathologies.

International Journal of Immunopathology and Pharmacology, 2011

The avoidance of food(s) is the main therapeutic approach to food allergy. Nevertheless, orally- ... more The avoidance of food(s) is the main therapeutic approach to food allergy. Nevertheless, orally- or sublingually-administered food allergens have gained attention and a number of food-allergic children can tolerate gradually increasing amounts of cow's milk and hen's egg. Our purpose is to show that oral desensitisation with food is an allergen-specific therapeutic approach and for this, we describe 4 illustrative children with IgE-mediated food allergy. The first was allergic to cow's milk and hen's egg, the second to cow's milk, hen's egg and fish. Both underwent oral desensitisation to both cow's milk and hen's egg. The third child was allergic to cow's milk, hen's egg and fish and underwent oral desensitisation with cow's milk. The last child was allergic to raw but not to cooked/boiled hen's egg and underwent the oral desensitisation with hen's egg. The first 2 children reached the clinical tolerance to cow's milk after th...

International Journal of Immunopathology and Pharmacology, 2011

Vγ9Vδ2 T lymphocytes have been shown to respond to a variety of non-peptide antigens including al... more Vγ9Vδ2 T lymphocytes have been shown to respond to a variety of non-peptide antigens including alkylamines and phosphoantigens. Recently, aminobisphosphonates have also been shown to stimulate this subset of γδ+ T cells. In this study we analyzed the proliferative responses of freshly isolated γδ T lymphocytes obtained from human cord blood when challenged with pyrophosphomonoesters or aminobisphosphonates. Nitrogen-containing aminobisphopsphonates, in contrast to phoshoantigens, readily stimulated expansion of Vδ2Vγ9 cells in human cord blood. Expanded cells displayed an activated mature phenotype, and were capable of producing TNFα and IFNγ but not perforin following secondary stimulation, consistent with the development of a regulatory, as opposed to cytotoxic, phenotype. This approach may provide a useful strategy for a new approach to the treatment of neonatal pathologies.

In newborn the innate immune system provides essential protection during primary infections befor... more In newborn the innate immune system provides essential protection during primary infections before the generation of an appropriate adaptive immune response that is initially not fully operative. Innate immune response is evoked and perpetuated by molecules derived from microorganisms or by the damage/death of host cells. These are collectively known as damage-associated molecular-pattern (DAMP) molecules. High-mobility group box 1 protein (HMGB1) or amphoterin, which previously was considered to be only a nuclear factor, has been recently identified as a DAMP molecule. When it is actively secreted by inflammatory cells or passively released from necrotic cells, HMGB1 mediates the response to infection, injury and inflammation, inducing dendritic cells maturation and T helper-1-cell responses. To characterize the role of HMGB1 in the innate and immature defense mechanisms in newborns, human cord blood (CB) mononuclear cells, in comparison to adult peripheral blood (PB) mononuclear c...

The mean neonatal and maternal serum level of anti-tetanus IgG was 3.04 ± 2.47 IU/mL and 3.4 ± 2.... more The mean neonatal and maternal serum level of anti-tetanus IgG was 3.04 ± 2.47 IU/mL and 3.4 ± 2.62 IU/mL, respectively. These levels were not statistically significant. A highly significant correlation was observed between maternal and neonatal anti-tetanus IgG (r = 0.88), as the rate of antitoxin of the infants increased by each 1 IU/mL of the mothers. In total, 95.4% of mothers and 97.7% newborns had protective level (> 0.1 IU/mL) for anti-tetanus IgG. The mean neonatal/maternal blood ratio of anti-tetanus IgG was 0.94 ± 0.57 (0.17-4.33).

Pediatric Allergy and Immunology, 2012

To cite this article: Meglio P, Giampietro PG, Carello R, Gabriele I, Avitabile S, Galli E. Oral ... more To cite this article: Meglio P, Giampietro PG, Carello R, Gabriele I, Avitabile S, Galli E. Oral food desensitization in children with IgE-mediated hen's egg allergy:

Early Human Development, 2008

The mean neonatal and maternal serum level of anti-tetanus IgG was 3.04 ± 2.47 IU/mL and 3.4 ± 2.... more The mean neonatal and maternal serum level of anti-tetanus IgG was 3.04 ± 2.47 IU/mL and 3.4 ± 2.62 IU/mL, respectively. These levels were not statistically significant. A highly significant correlation was observed between maternal and neonatal anti-tetanus IgG (r = 0.88), as the rate of antitoxin of the infants increased by each 1 IU/mL of the mothers. In total, 95.4% of mothers and 97.7% newborns had protective level (> 0.1 IU/mL) for anti-tetanus IgG. The mean neonatal/maternal blood ratio of anti-tetanus IgG was 0.94 ± 0.57 (0.17-4.33).

PLoS ONE, 2011

In newborn the innate immune system provides essential protection during primary infections befor... more In newborn the innate immune system provides essential protection during primary infections before the generation of an appropriate adaptive immune response that is initially not fully operative. Innate immune response is evoked and perpetuated by molecules derived from microorganisms or by the damage/death of host cells. These are collectively known as damage-associated molecular-pattern (DAMP) molecules. High-mobility group box 1 protein (HMGB1) or amphoterin, which previously was considered to be only a nuclear factor, has been recently identified as a DAMP molecule. When it is actively secreted by inflammatory cells or passively released from necrotic cells, HMGB1 mediates the response to infection, injury and inflammation, inducing dendritic cells maturation and T helper-1-cell responses. To characterize the role of HMGB1 in the innate and immature defense mechanisms in newborns, human cord blood (CB) mononuclear cells, in comparison to adult peripheral blood (PB) mononuclear cells, have been analyzed for its expression. By flow cytometry and western blot analysis, we observed that in CB and PB cells: i) HMGB1 is expressed on cell surface membranes of myeloid dendritic cell precursors, mostly, and lymphocytes (gamma/delta and CD4(+) T cells) to a lesser extent; ii) different pro-inflammatory stimuli or molecules that mimic infection increased cell surface expression of HMGB1 as well as its secretion into extracellular environment; iii) the treatment with synthetic molecules such as aminobisphosphonates (ABs), identified to be γδ T cell antigens, triggered up-regulation of HMGB1 expression on mononuclear cells, as well γδ T lymphocytes, inducing its secretion. The modulation of its secretion and the HMGB1-mediated migration of monocytes indicated HMGB1 as regulator of immune response in an immature system, like CB, through engagement of γδ T lymphocytes and myeloid dendritic cell precursors, essential components of innate immunity. In addition, the increased HMGB1 expression/secretion triggered by ABs, previously characterized for their immuno-modulating and immune-adjuvant capabilities, indicated that immunomodulation might represent a new therapeutical approach for neonatal and adult pathologies.