Idoia Pujana - Academia.edu (original) (raw)

Papers by Idoia Pujana

Journal of clinical microbiology, 1999

PCR fingerprinting was used for the epidemiological investigation of 64 Pseudomonas aeruginosa is... more PCR fingerprinting was used for the epidemiological investigation of 64 Pseudomonas aeruginosa isolates collected from 16 chronic bronchiectasis patients without cystic fibrosis: 56% of the patients harbored one clone, 12.5% carried a single major type with minor variants, and 31.5% carried two clones. Only a minority of the acquisitions of antibiotic resistance was related to the acquisition of exogenous strains. Mucoid and nonmucoid sets of isolates did not display any consistent differences in their patterns. The genetic similarity among the clones ranged from 10 to 69%. Cross-infection or common-source exposure did not appear to have occurred.

Journal of Microbiological Methods

tion of several large fragments, e.g. primers reading out of repeats repMP1 (forward) and out of ... more tion of several large fragments, e.g. primers reading out of repeats repMP1 (forward) and out of repMP5 (reversed) should yield fragments of 5.4, 5.6, 14.7, and 19.8 kb. The PCR products formed, or restriction patterns of these fragments should allow discrimination of M. pneumoniae strains.Initialy, 4 M. pneumo

Microbiology, 1996

Nisin is a small post-translationally modified lanthionine-containing peptide (lantibiotic) produ... more Nisin is a small post-translationally modified lanthionine-containing peptide (lantibiotic) produced by certain Lactococcus lactis strains which has a high antimicrobial activity against several pathogenic Gram-positive bacteria. Northern blots and RT/PCR analyses of the nisin-producing strain N8 revealed that the nisZBTCIPRKFEG gene cluster, responsible for nisin biosynthesis, immunity and regulation, consists of two operons, nisZBTCIPRK and nisFEG. The promoter of the nisFEG operon was mapped. The −35 to −1 region upstream of the transcription start of the nisFEG promoter showed 73% identity with the corresponding region upstream of the nisA and nisZ gene. In contrast to earlier reports, nisin was found to be secreted during the early stages of growth as well as later in the growth cycle. The secreted nisin was adsorbed on the surface of the cells and was released to the medium during mid-exponential growth, when the pH in the medium fell below 5.5. In nisZB antisense and nisT del...

Enferm Infect Microbiol …, 2004

Carbapenemase detection in Acinetobacter baumannii clones resistant to imipenem INTRODUCTION. The... more Carbapenemase detection in Acinetobacter baumannii clones resistant to imipenem INTRODUCTION. The aim of this study was to detect carbapenemases in imipenem-resistant Acinetobacter baumannii isolates obtained in the microbiology department of a Basque Country Public Health Service hospital over a period of 19 months, and to genetically characterize the resistant clones. METHODS. Susceptibility tests to imipenem, meropenem, ticarcillin, ceftazidime, cefotaxime, cefepime and aztreonam were done by determining the minimum inhibitory concentration on agar plates. A tRNA technique was used for species identification and PCR with primers ERIC2, AP3 and M13 for genetic typing of resistant isolates. Carbapenemase production was detected by the Hodge test and metallo-beta-lactamase by the EDTA test and Etest MBL. RESULTS. A total of 76 isolates were resistant to imipenem and 49 of these were resistant to all the betalactam antibiotics tested. Genetic typing showed three predominant clones, denominated I (9 isolates), II (48 isolates) and III (8 isolates). Hodge and EDTA tests were positive in 45 and 8 isolates belonging to clone II, 8 and 4 belonging to clone I and 7 and 3 belonging to clone III, respectively. The Etest confirmed 7 results (45% of the 17 positive EDTA test isolates). CONCLUSION. Our results show that one factor contributing to the high level of imipenem resistance in the isolates analyzed is dissemination of a predominant, multiresistant clone able to produce OXA-type carbapenemases and metallo-beta-lactamases.

… microbiology and infectious …, 2000

Seventeen multiple-antibiotic-resistant Pseudomonas aeruginosa isolates were collected from two p... more Seventeen multiple-antibiotic-resistant Pseudomonas aeruginosa isolates were collected from two patients hospitalized in the same intensive care unit. They showed a parallel acquisition of resistance to antibiotics and they were, therefore, thought to have a common clonal ...

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