Ieda Millas - Academia.edu (original) (raw)
Papers by Ieda Millas
The Anatomical Record
Estrogen is an important hormone for health in both genders. It is indispensable to glucose homeo... more Estrogen is an important hormone for health in both genders. It is indispensable to glucose homeostasis, immune robustness, bone health, cardiovascular health, and neural functions. The main way that estrogen acts in the cells is through estrogen receptors (ERs). The presence of specific estrogen receptors is required for estrogen to have its characteristic ubiquitous action in almost all tissues. Estrogen receptor alpha (ERα) and estrogen receptor beta (ERβ) are the major isoforms of estrogen that are highly specific in humans and enable selective hormonal actions in different tissues. This article reviews some of the observed estrogen actions and effects in different tissues and cells through these specific receptors. This ubiquitous, almost ordinary hormone may reveal itself as a significant factor that helped us to better understand the complexity of the human immune system response against respiratory infections, including the COVID‐19, and especially in the current state of this painful pandemic.
Site: www.bjorl.org.br Brazilian Journal of Otorhinolaryngology é uma publicação oficial da Assoc... more Site: www.bjorl.org.br Brazilian Journal of Otorhinolaryngology é uma publicação oficial da Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial (ABORL-CCF) em parceria com a Elsevier Ltda. e é dedicada a comunidade médica. A versão impressa e eletrônica da Brazilian Journal of Otorhinolaryngology é publicada em língua inglesa e portuguesa. Brazilian Journal of Otorhinolaryngology é filiada à MEDLINE, Exerpta Medica, LILACS (Index Medicus Latinoanericano), Scielo (Scientific Electronic Library Online), Thomson Scientific (ISI). Fator de Impacto 2013: 0,623 Os artigos não podem ser transcritos no todo ou em partes. A edição regular será de seis números anuais, em fevereiro, abril, junho, agosto, outubro e dezembro. Distribuída gratuitamente aos sócios da ABORL-CCF. Para assinatura, contatar a Secretaria da ABORL-CCF. A revista não se responsabiliza pela veracidade dos dados apresentados pelos autores. © 2014 Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial. Publicada pela Elsevier Editora Ltda. Todos os direitos reservados.
Revista Brasileira de Otorrinolaringologia, 2002
Brazilian journal of otorhinolaryngology
Some clinical trials revealed a correlation between increased serum estrogen and nasal symptoms o... more Some clinical trials revealed a correlation between increased serum estrogen and nasal symptoms or inflammatory changes in nasal mucosa. Estrogen receptors tend to be controlled by a negative feedback, to avoid a deleterious stimulus over several body functions while in hyperestrogenic periods. This study proposes a hypothesis where mechanisms regulating expression of estradiol receptors in nasal mucosa are absent in some patients, and their concentration remains steady even in periods of high serum hormonal concentration, potentially leading to local estrogenic symptoms in nasal mucosa. This was an experimental prospective study. To determine whether estrogen levels induce the reduction of the number of estrogen receptors in the nasal mucosa. In the present study, 30 adult male guinea pigs were subjected to a biopsy of the middle nasal turbinate and received 0.5 mL of estradiol cypionate intraperitoneally for 30 consecutive days. Afterwards, samples from contralateral middle turbin...
International Journal of Morphology, 2014
ABSTRACT Immunohistochemistry is a specific and sensitive staining method for detection of severa... more ABSTRACT Immunohistochemistry is a specific and sensitive staining method for detection of several proteins. One important function of this method is to help us on the diagnosis of the presence of specific receptors as the estrogen receptors. The aim of this study was to compare two different methods to evaluate immunohistochemical staining intensity to detect the presence of ER in the nasal mucosa tissue: one using a digital system and the other through conventional direct microscopy observation. Sixty two stained samples were observed and analyzed under optic microscopy by three specialized professionals, who have graded intensity from: absent, mild, moderate and intense staining. Afterwards, an objective measurement was obtained by a relative optical density (ROD) reading, through the MCID 7.0 system of densitometric digital analysis (Inter Focus Imaging LTDA, Linton, England). Subjective and objective classifications were compared under statistical analysis (Kolmogorov-Smirnov and Spearman Correlation Test, p<0.05). We found a positive correlation between the subjective findings of observers and digital analysis in all the categories of beta receptor. For the alpha receptor, there was a correlation only in extreme categories. The subjective evaluations by observers and digital method by measuring the relative optical density show statistically significant correlations in quantifying the estrogen receptors by immunohistochemistry staining. This indicates that both methods show accuracy for the proposed study.
Brazilian Journal of Otorhinolaryngology (Impresso), 2009
Nasal turbinates play an important role in nasal physiology. These functions include the importan... more Nasal turbinates play an important role in nasal physiology. These functions include the important function of particle filtration by the mucocilliary system. Many nasal mucosal diseases, such as rhinitis and rhinosinusitis, are directly related with structural alterations of the mucosal lining of the turbinates. To study the distribution pattern of the glandular epithelium of the lamina propria in the normal lower nasal turbinate mucosa of the anterior, medium and posterior portions. A prospective study in which small linear fragment of the lower nasal turbinate was removed from ten patients undergoing aesthetic nose surgery. The slides were hematoxilin-eosin stained, examined histologically and photographed. Glandular epithelium was delimited individually, the total area of the lamina propria on the anterior, medium and posterior portions of nasal turbinates was calculated (microm2). There was no statistically significant difference in the distribution pattern of the glandular epithelium of the lamina propria. This study showed no predominance of glandular epithelium distribution in anterior and posterior portions of lower nasal turbinates in normal subjects.
International Journal of Morphology, 2010
It has been postulated that the nasal mucosa, like other human tissues, is affected by a complex ... more It has been postulated that the nasal mucosa, like other human tissues, is affected by a complex interactive network of neuropeptides, cytokines, allergic and inflammatory mediators and hormones such as estrogen, in which associations between symptoms (e.g. nasal stuffiness and coryza) and hormonal variations deriving from pregnancy, use of hormonal contraceptives and menstrual cycle phases are observed. The objective is evaluating the presence of specific estrogen receptors (types alpha and beta) in inferior turbinate mucosa in healthy subjects without nasal symptoms. Samples of nasal inferior turbinate were removed from patients undergoing aesthetic nasal surgery, and analyzed using hematoxylin-eosin staining, followed by immunohistochemical preparations on paraffin-embedded sections from the material sample, to detect estrogen receptors alpha and beta. Positive immunohistochemical reactions for both beta
Revista Brasileira de Otorrinolaringologia, 2002
Introdução: os enxertos de cartilagem começaram a ser usados por Jansen em 1963 que sugeriu que t... more Introdução: os enxertos de cartilagem começaram a ser usados por Jansen em 1963 que sugeriu que teriam sua indicação em casos mais avançados por ser mais rígido resistindo melhor à reabsorções e retrações. Em 1998 Eavey descreve o uso de cartilagem tragal com pericôndrio bilateral e a sua colocação sem incisões no meato acústico externo. Forma de estudo: clínico prospectivo randomizado. material e método: Foram estudados 100 pacientes portadores de seqüelas de otites médias crônicas, com perfurações da membrana timpânica com rebordo e submetidos a timpanoplastias com cartilagem tragal e pericôndrio. As dimensões das perfurações foram em média 40% da área da membrana timpânica. Na avaliação auditiva observamos média pré-operatória de perda condutiva de 28.3 dB e pósoperatória de 9.8 dB. Resultados: O fechamento da perfuração foi total em 95.0% dos casos. Conclusão: Estudando os resultados concluímos que o método é de fácil realização, com poucas complicações, bons resultados auditivos e ótimos resultados anatômicos. Sendo uma ótima opção para o otologista na resolução das seqüelas das otites crônicas.
Otolaryngology -- Head and Neck Surgery, 2011
Head & Neck, 2002
Background. Eosinophilic angiocentric fibrosis (EAF) is a rare fibroinflammatory lesion of the si... more Background. Eosinophilic angiocentric fibrosis (EAF) is a rare fibroinflammatory lesion of the sinonasal tract that occurs mainly in young to middle-aged female patients. Only two previous cases affecting male patients have been reported, and its etiopathogenesis remains unknown. The authors report on the third case of the entity in a male patient and review the 12 previously reported cases.
Endocrinology, 1988
Plasma levels of atrial natriuretic peptide (ANP) have been measured in eight sodium-retaining ci... more Plasma levels of atrial natriuretic peptide (ANP) have been measured in eight sodium-retaining cirrhotic nonascitic rats and eight control animals before and after extracellular volume expansion by isotonic saline infusion (30 ml/kg BW, 20 min). In addition, disappearance of [125I]ANP was studied in six control and six cirrhotic rats. The effect of infusing synthetic rat ANP-(1-28) (1 microgram) on mean arterial pressure and renal function has been also studied. In basal conditions, cirrhotic rats showed higher ANP plasma levels than control animals (71.1 +/- 16.6 vs. 43.9 +/- 5.1 pg/ml; P less than 0.05). After extracellular volume expansion, ANP increased in both control and cirrhotic rats; the increase in cirrhotic was higher than that in control rats (88 +/- 27% vs. 33 +/- 8%; P less than 0.05). Disappearance of iodinated ANP from plasma was identical in control and cirrhotic rats. ANP infusion induced a larger decrease in mean arterial pressure in control (21 +/- 5%) than in cirrhotic rats (9 +/- 2.5%). ANP induced comparable increases in glomerular filtration rate and renal plasma flow in both groups of animals. However, diuretic and natriuretic effects were markedly impaired in cirrhotic animals. Thus, urinary flow increased by 91 +/- 18 microliters/min in control animals, but only by 37 +/- 7 microliters/min in cirrhotic animals. Fractional sodium excretion increased to 1.7 +/- 0.44% in controls and to 0.54 +/- 0.12% in cirrhotic rats (P less than 0.05). It is concluded that the defect in renal handling of sodium in cirrhotic rats is not due to a lack of ANP synthesis or release. In addition, these animals show an impaired renal response to ANP.
Contraception, 2011
Background: There is evidence that the nasal mucosa is affected by estrogen influence. Some autho... more Background: There is evidence that the nasal mucosa is affected by estrogen influence. Some authors have already detected estrogen 13 receptors (ER) in the nasal mucosa. However, there doesn't seem to be a consensus about the concentration and distribution of the ER or the 14 possible influence of hormonal contraceptives in the nasal mucosa.
The Anatomical Record
Estrogen is an important hormone for health in both genders. It is indispensable to glucose homeo... more Estrogen is an important hormone for health in both genders. It is indispensable to glucose homeostasis, immune robustness, bone health, cardiovascular health, and neural functions. The main way that estrogen acts in the cells is through estrogen receptors (ERs). The presence of specific estrogen receptors is required for estrogen to have its characteristic ubiquitous action in almost all tissues. Estrogen receptor alpha (ERα) and estrogen receptor beta (ERβ) are the major isoforms of estrogen that are highly specific in humans and enable selective hormonal actions in different tissues. This article reviews some of the observed estrogen actions and effects in different tissues and cells through these specific receptors. This ubiquitous, almost ordinary hormone may reveal itself as a significant factor that helped us to better understand the complexity of the human immune system response against respiratory infections, including the COVID‐19, and especially in the current state of this painful pandemic.
Site: www.bjorl.org.br Brazilian Journal of Otorhinolaryngology é uma publicação oficial da Assoc... more Site: www.bjorl.org.br Brazilian Journal of Otorhinolaryngology é uma publicação oficial da Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial (ABORL-CCF) em parceria com a Elsevier Ltda. e é dedicada a comunidade médica. A versão impressa e eletrônica da Brazilian Journal of Otorhinolaryngology é publicada em língua inglesa e portuguesa. Brazilian Journal of Otorhinolaryngology é filiada à MEDLINE, Exerpta Medica, LILACS (Index Medicus Latinoanericano), Scielo (Scientific Electronic Library Online), Thomson Scientific (ISI). Fator de Impacto 2013: 0,623 Os artigos não podem ser transcritos no todo ou em partes. A edição regular será de seis números anuais, em fevereiro, abril, junho, agosto, outubro e dezembro. Distribuída gratuitamente aos sócios da ABORL-CCF. Para assinatura, contatar a Secretaria da ABORL-CCF. A revista não se responsabiliza pela veracidade dos dados apresentados pelos autores. © 2014 Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial. Publicada pela Elsevier Editora Ltda. Todos os direitos reservados.
Revista Brasileira de Otorrinolaringologia, 2002
Brazilian journal of otorhinolaryngology
Some clinical trials revealed a correlation between increased serum estrogen and nasal symptoms o... more Some clinical trials revealed a correlation between increased serum estrogen and nasal symptoms or inflammatory changes in nasal mucosa. Estrogen receptors tend to be controlled by a negative feedback, to avoid a deleterious stimulus over several body functions while in hyperestrogenic periods. This study proposes a hypothesis where mechanisms regulating expression of estradiol receptors in nasal mucosa are absent in some patients, and their concentration remains steady even in periods of high serum hormonal concentration, potentially leading to local estrogenic symptoms in nasal mucosa. This was an experimental prospective study. To determine whether estrogen levels induce the reduction of the number of estrogen receptors in the nasal mucosa. In the present study, 30 adult male guinea pigs were subjected to a biopsy of the middle nasal turbinate and received 0.5 mL of estradiol cypionate intraperitoneally for 30 consecutive days. Afterwards, samples from contralateral middle turbin...
International Journal of Morphology, 2014
ABSTRACT Immunohistochemistry is a specific and sensitive staining method for detection of severa... more ABSTRACT Immunohistochemistry is a specific and sensitive staining method for detection of several proteins. One important function of this method is to help us on the diagnosis of the presence of specific receptors as the estrogen receptors. The aim of this study was to compare two different methods to evaluate immunohistochemical staining intensity to detect the presence of ER in the nasal mucosa tissue: one using a digital system and the other through conventional direct microscopy observation. Sixty two stained samples were observed and analyzed under optic microscopy by three specialized professionals, who have graded intensity from: absent, mild, moderate and intense staining. Afterwards, an objective measurement was obtained by a relative optical density (ROD) reading, through the MCID 7.0 system of densitometric digital analysis (Inter Focus Imaging LTDA, Linton, England). Subjective and objective classifications were compared under statistical analysis (Kolmogorov-Smirnov and Spearman Correlation Test, p<0.05). We found a positive correlation between the subjective findings of observers and digital analysis in all the categories of beta receptor. For the alpha receptor, there was a correlation only in extreme categories. The subjective evaluations by observers and digital method by measuring the relative optical density show statistically significant correlations in quantifying the estrogen receptors by immunohistochemistry staining. This indicates that both methods show accuracy for the proposed study.
Brazilian Journal of Otorhinolaryngology (Impresso), 2009
Nasal turbinates play an important role in nasal physiology. These functions include the importan... more Nasal turbinates play an important role in nasal physiology. These functions include the important function of particle filtration by the mucocilliary system. Many nasal mucosal diseases, such as rhinitis and rhinosinusitis, are directly related with structural alterations of the mucosal lining of the turbinates. To study the distribution pattern of the glandular epithelium of the lamina propria in the normal lower nasal turbinate mucosa of the anterior, medium and posterior portions. A prospective study in which small linear fragment of the lower nasal turbinate was removed from ten patients undergoing aesthetic nose surgery. The slides were hematoxilin-eosin stained, examined histologically and photographed. Glandular epithelium was delimited individually, the total area of the lamina propria on the anterior, medium and posterior portions of nasal turbinates was calculated (microm2). There was no statistically significant difference in the distribution pattern of the glandular epithelium of the lamina propria. This study showed no predominance of glandular epithelium distribution in anterior and posterior portions of lower nasal turbinates in normal subjects.
International Journal of Morphology, 2010
It has been postulated that the nasal mucosa, like other human tissues, is affected by a complex ... more It has been postulated that the nasal mucosa, like other human tissues, is affected by a complex interactive network of neuropeptides, cytokines, allergic and inflammatory mediators and hormones such as estrogen, in which associations between symptoms (e.g. nasal stuffiness and coryza) and hormonal variations deriving from pregnancy, use of hormonal contraceptives and menstrual cycle phases are observed. The objective is evaluating the presence of specific estrogen receptors (types alpha and beta) in inferior turbinate mucosa in healthy subjects without nasal symptoms. Samples of nasal inferior turbinate were removed from patients undergoing aesthetic nasal surgery, and analyzed using hematoxylin-eosin staining, followed by immunohistochemical preparations on paraffin-embedded sections from the material sample, to detect estrogen receptors alpha and beta. Positive immunohistochemical reactions for both beta
Revista Brasileira de Otorrinolaringologia, 2002
Introdução: os enxertos de cartilagem começaram a ser usados por Jansen em 1963 que sugeriu que t... more Introdução: os enxertos de cartilagem começaram a ser usados por Jansen em 1963 que sugeriu que teriam sua indicação em casos mais avançados por ser mais rígido resistindo melhor à reabsorções e retrações. Em 1998 Eavey descreve o uso de cartilagem tragal com pericôndrio bilateral e a sua colocação sem incisões no meato acústico externo. Forma de estudo: clínico prospectivo randomizado. material e método: Foram estudados 100 pacientes portadores de seqüelas de otites médias crônicas, com perfurações da membrana timpânica com rebordo e submetidos a timpanoplastias com cartilagem tragal e pericôndrio. As dimensões das perfurações foram em média 40% da área da membrana timpânica. Na avaliação auditiva observamos média pré-operatória de perda condutiva de 28.3 dB e pósoperatória de 9.8 dB. Resultados: O fechamento da perfuração foi total em 95.0% dos casos. Conclusão: Estudando os resultados concluímos que o método é de fácil realização, com poucas complicações, bons resultados auditivos e ótimos resultados anatômicos. Sendo uma ótima opção para o otologista na resolução das seqüelas das otites crônicas.
Otolaryngology -- Head and Neck Surgery, 2011
Head & Neck, 2002
Background. Eosinophilic angiocentric fibrosis (EAF) is a rare fibroinflammatory lesion of the si... more Background. Eosinophilic angiocentric fibrosis (EAF) is a rare fibroinflammatory lesion of the sinonasal tract that occurs mainly in young to middle-aged female patients. Only two previous cases affecting male patients have been reported, and its etiopathogenesis remains unknown. The authors report on the third case of the entity in a male patient and review the 12 previously reported cases.
Endocrinology, 1988
Plasma levels of atrial natriuretic peptide (ANP) have been measured in eight sodium-retaining ci... more Plasma levels of atrial natriuretic peptide (ANP) have been measured in eight sodium-retaining cirrhotic nonascitic rats and eight control animals before and after extracellular volume expansion by isotonic saline infusion (30 ml/kg BW, 20 min). In addition, disappearance of [125I]ANP was studied in six control and six cirrhotic rats. The effect of infusing synthetic rat ANP-(1-28) (1 microgram) on mean arterial pressure and renal function has been also studied. In basal conditions, cirrhotic rats showed higher ANP plasma levels than control animals (71.1 +/- 16.6 vs. 43.9 +/- 5.1 pg/ml; P less than 0.05). After extracellular volume expansion, ANP increased in both control and cirrhotic rats; the increase in cirrhotic was higher than that in control rats (88 +/- 27% vs. 33 +/- 8%; P less than 0.05). Disappearance of iodinated ANP from plasma was identical in control and cirrhotic rats. ANP infusion induced a larger decrease in mean arterial pressure in control (21 +/- 5%) than in cirrhotic rats (9 +/- 2.5%). ANP induced comparable increases in glomerular filtration rate and renal plasma flow in both groups of animals. However, diuretic and natriuretic effects were markedly impaired in cirrhotic animals. Thus, urinary flow increased by 91 +/- 18 microliters/min in control animals, but only by 37 +/- 7 microliters/min in cirrhotic animals. Fractional sodium excretion increased to 1.7 +/- 0.44% in controls and to 0.54 +/- 0.12% in cirrhotic rats (P less than 0.05). It is concluded that the defect in renal handling of sodium in cirrhotic rats is not due to a lack of ANP synthesis or release. In addition, these animals show an impaired renal response to ANP.
Contraception, 2011
Background: There is evidence that the nasal mucosa is affected by estrogen influence. Some autho... more Background: There is evidence that the nasal mucosa is affected by estrogen influence. Some authors have already detected estrogen 13 receptors (ER) in the nasal mucosa. However, there doesn't seem to be a consensus about the concentration and distribution of the ER or the 14 possible influence of hormonal contraceptives in the nasal mucosa.