Iga Hołyńska-Iwan - Academia.edu (original) (raw)
Papers by Iga Hołyńska-Iwan
Postępy Higieny i Medycyny Doświadczalnej
Streszczenie Pyretroidy, syntetyczne pochodne naturalnych pyretryn pochodzących z Chrysanthemum c... more Streszczenie Pyretroidy, syntetyczne pochodne naturalnych pyretryn pochodzących z Chrysanthemum cinerariaefolim, powszechnie stosowane do ochrony roślin, w leśnictwie, przemyśle rolniczym, farmaceutycznym, a także w medycynie oraz weterynarii. Do organizmu mogą się dostać poprzez wdychanie, spożycie oraz kontakt ze skórą. Przyjęto, że charakteryzują się niewielką toksycznością dla ludzi, są szybko metabolizowane i nie kumulują się w tkankach, a wydalane są z moczem. Mimo istniejących licznych obostrzeń, ich stosowanie niesie duże ryzyko, ponieważ związki te oraz ich metabolity mogą przedostawać się do środowiska naturalnego, zanieczyszczając wodę, glebę oraz żywność. Od wielu lat opisywane są skutki stosowania pyretroidów jako bezpośrednie zagrożenie dla zdrowia zwierząt oraz ludzi. Na bieżąco publikowane są informuje o zatruciach tymi związkami u ludzi i zwierząt oraz o ofiarach śmiertelnych po ich zażyciu. Najbardziej narażone są dzieci, gdyż pyretroidy mogą występować w mleku mat...
Diagnostyka Laboratoryjna, Apr 13, 2018
Cortisol, also called “the” stress hormone is a glucocorticoid secreted by the adrenal cortex. Th... more Cortisol, also called “the” stress hormone is a glucocorticoid secreted by the adrenal cortex. This hormone plays a significant role in maintaining homeostasis, according to the body’s total stress. Cortisol interferes with many organs, affects glucose and fatty acids metabolism and neurotransmitter secretion. Predominantly, cortisol influences the carbohydrate metabolism, stimulating gluconeogenesis in the liver and inhibiting glucose utilization in peripheral tissues. As it is an element “fight or flight” it also stimulates central nervous system and enhances blood flow. To some extent cortisol influences also the renal handling of electrolytes, namely: increasing sodium resorption, and renal excretion of potassium, calcium and phosphates. Through its anti-inflammatory and immunosuppressive character this glucocorticoid modulates the immune system functioning. Cortisol has a circadian rhythm following ACTH (adrenocorticotropic hormone) secretion. Increased cortisol levels are observed physiologically during stress and pathologically in Cushing’s syndrome. Chronic hypercortisolism is harmful or the body, and its effects present an extremely wide spectrum, including insulin resistance, obesity, insomnia and even depression. Thus, laboratory diagnosis of cortisol level is important for the diagnosis, monitoring and evaluate the effectiveness of hypercortisolism treatment. Słowa kluczowe: hiperkortyzolemia, oznaczanie kortyzolu, reakcja stresowa;
Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego, Sep 29, 2017
Based on the analysis of data from clinical trials it could be postulated that N-acetylcysteine h... more Based on the analysis of data from clinical trials it could be postulated that N-acetylcysteine has a positive impact on the treatment of various diseases. However, less is known about specific molecular and physiological mechanisms underlying the reported therapeutic effects. N-acetylcysteine (NAC, N-acetyl-L-cysteine) is an amino acid derivative containing a thiol group. It is a precursor of L-cysteine and glutathione. NAC is well absorbed and safe for the body at doses up to 300 mg per kg of body weight. Side effects are relatively rare. NAC is used as an mucolytic agent in adjunctive therapy of respiratory diseases causing the retention of secretions, as well as an antidote in the treatment of paracetamol poisoning. Moreover, NAC protects against the toxic effects of reactive oxygen species and their active metabolites. NAC is involved in free radical scavenging processes via several independent mechanisms, including a direct reduction of free radicals, providing substrates for ...
Postȩpy higieny i medycyny doświadczalnej, 2021
Pyrethroids, synthetic derivatives of natural pyrethrins derived from Chrysanthemum cinerariaefol... more Pyrethroids, synthetic derivatives of natural pyrethrins derived from Chrysanthemum cinerariaefolim, are commonly used for plant protection in the forestry, agricultural, pharmaceutical industry as well as in medicine and veterinary medicine. They can enter the body by inhalation, ingestion and skin contact. It was assumed that they are characterized by low toxicity to humans, are quickly metabolized and do not accumulate in tissues, and are excreted in the urine. Despite the existing restrictions, their use carries a great risk, because these compounds and their metabolites can get into the natural environment, contaminating water, soil and food. The consequences of using pyrethroids as a direct threat to animal and human health have been described for many years. They are published on an ongoing basis informing about poisoning with these compounds in humans and animals, and about fatalities after their taking. Children are most at risk because pyrethroids can be found in breast mi...
Medicina
Pyrethroids are pesticides commonly used in crop protection; in the forestry, wood, and textile i... more Pyrethroids are pesticides commonly used in crop protection; in the forestry, wood, and textile industries; as well as in medicine and veterinary medicine to treat parasitic crustacean infestations. They have been found to be relatively safe for humans and animals. Pyrethroids are recommended for personal protection against malaria and virus Zika by the World Health Organization. Pyrethroids act on voltage-gated sodium channels, which cause an influx of sodium ions into the nerve cells and permanent depolarization. They also influence activities of enzymes, especially in nerve and liver cells. Contact of pyrethroids with the skin, digestive tract, and respiratory tract results in their penetration into the body. Due to the importance of the subject, a summary of the current state of knowledge on the toxic effects of pyrethroids was presented in the comprehensive review by Chrustek et al, published in journal Medicina. Particular attention was paid to nephrotoxic, hepatotoxic, cardio...
Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego, 2017
Based on the analysis of data from clinical trials it could be postulated that N-acetylcysteine h... more Based on the analysis of data from clinical trials it could be postulated that N-acetylcysteine has a positive impact on the treatment of various diseases. However, less is known about specific molecular and physiological mechanisms underlying the reported therapeutic effects. N-acetylcysteine (NAC, N-acetyl-L-cysteine) is an amino acid derivative containing a thiol group. It is a precursor of L-cysteine and glutathione. NAC is well absorbed and safe for the body at doses up to 300 mg per kg of body weight. Side effects are relatively rare. NAC is used as an mucolytic agent in adjunctive therapy of respiratory diseases causing the retention of secretions, as well as an antidote in the treatment of paracetamol poisoning. Moreover, NAC protects against the toxic effects of reactive oxygen species and their active metabolites. NAC is involved in free radical scavenging processes via several independent mechanisms, including a direct reduction of free radicals, providing substrates for ...
Pyrethroids are pesticides commonly used in crop protection; in the forestry, wood, and textile i... more Pyrethroids are pesticides commonly used in crop protection; in the forestry, wood, and textile industries; as well as in medicine and veterinary medicine to treat parasitic crustacean infestations. They have been found to be relatively safe for humans and animals. Pyrethroids are recommended for personal protection against malaria and virus Zika by the World Health Organization. Pyrethroids act on voltage-gated sodium channels, which cause an influx of sodium ions into the nerve cells and permanent depolarization. They also influence activities of enzymes, especially in nerve and liver cells. Contact of pyrethroids with the skin, digestive tract, and respiratory tract results in their penetration into the body. Due to the importance of the subject, a summary of the current state of knowledge on the toxic effects of pyrethroids was presented in the comprehensive review by Chrustek et al, published in journal Medicina. Particular attention was paid to nephrotoxic, hepatotoxic, cardio...
Cancers
Ovarian cancer is one of the most common malignancies among women worldwide. The course of the di... more Ovarian cancer is one of the most common malignancies among women worldwide. The course of the disease is often latent and asymptomatic in the early stages, but as it develops, metastasis occurs, accompanied by accumulation of ascites in the peritoneal cavity. The ascites fluid constitutes a specific microenvironment influencing the processes of carcinogenesis. In ascites, signaling is mediated by various cytokines that control tumor cell proliferation, progression, metastasis, and chemoresistance. Adipokines, secreted into ascites and also appearing in blood, may be markers of ongoing processes related to the development of neoplastic disease. Moreover, a significant influence of adipocyte lipids on the growth of tumors, for which they are one of energy sources, is observed. Adiponectin, interleukin 6 (IL-6), interleukin 8 (IL-8), monocyte chemotactic protein-1 (MCP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1), discussed in the present review, were found to mediate the e...
PLOS ONE
The preservation of physiological transport of ions and water content is particularly important f... more The preservation of physiological transport of ions and water content is particularly important for maintaining the skin barrier, touch and pain stimuli, as well as the initiation of skin regeneration processes, especially after treatments associated with breaking skin continuity and wound healing difficulties. The aim of the study was to assess changes in ion transport, measured as values of transepithelial electric resistance and potential difference in stationary conditions and during mechanical-chemical stimulations, depending on the hydration status of isolated rabbit skin specimens. The specimens were divided into five groups: control (n = 22), dehydrated in 10% NaCl (n = 30), rehydrated after dehydration (n = 26), dried at 37°C (n = 26), and rehydrated after drying (n = 25). Dehydrated tissue samples showed altered resistance compared to the control; this change was maintained regardless of rehydration. In the dehydrated samples, changes in the measured electric potential wer...
Scientific Reports
The measurement of electric potential and resistance reflect the transport of sodium and chloride... more The measurement of electric potential and resistance reflect the transport of sodium and chloride ions which take place in keratinocytes and is associated with skin response to stimuli arising from external and internal environment. The aim of the study was to assess changes in electrical resistance and the transport of chloride and sodium ions, under iso-osmotic conditions and following the use of inhibitors affecting these ions’ transport, namely amiloride (A) and bumetanide (B). The experiment was performed on 104 fragments of rabbit skin, divided into three groups: control (n = 35), A—inhibited sodium transport (n = 33) and B—inhibited chloride transport (n = 36). Measurement of electrical resistance (R) and electrical potential (PD) confirmed tissue viability during the experiment, no statistically significant differences in relation to control conditions were noted. The minimal and maximal PD measured during stimulation confirmed the repeatability of the recorded reactions to ...
Skin Pharmacology and Physiology
Acute, adverse skin effects to capsaicin can be activated by inhibition of sodium transport not o... more Acute, adverse skin effects to capsaicin can be activated by inhibition of sodium transport not only in nociceptive neurons, but also in keratinocytes. The aim of the current study was to describe and compare immediate (15 s) and prolonged (30 min) effects of capsaicin on epidermal (not neural) sodium transport using a rabbit skin model. Skin fragments (n = 169) were incubated in 4 conditions: undisturbed ion transport (U; n = 48); inhibited sodium transport (INa; n = 34) with amiloride used as sodium transport blocker; long-term irritation by capsaicin with undisturbed ion transport (CAPSA-U; n = 43) and with inhibited sodium transport (CAPSA-INa; n = 35). After 30 min of incubation, a solution of capsaicin was applied directly to the skin fragments. The study demonstrated that sodium transport inhibition eliminated the effects of both immediate and prolonged capsaicin application. The results could be the basis for future research considering selective sodium transport inhibitors for human skin to reduce the side effects of capsaicin, related to activation of sodium channels in keratinocytes.
Biomedicine & Pharmacotherapy
Platinum-based drugs, used in treating tumors, cause numerous undesirable effects in patients, li... more Platinum-based drugs, used in treating tumors, cause numerous undesirable effects in patients, like neuropathic pain, hypersensitivity, reddening, pruritus and rash. Changes in Na+ transport modify local osmolality and contribute to the initiation of hypersensitivity and allergy. They are also associated with stimulation of C-fibres and hyperalgesia. Cl- transport is essential for regulation of sweat composition and the migration of immunocompetent cells. The aim of the conducted study was to assess the effect of a cisplatin solution on the electrophysiological parameters of the isolated rabbit skin specimens. The difference in transepithelial electrical potential (PD) and resistance (R) in stationary conditions and during 15 s mechanical-chemical stimulation (PDmin and PDmax), were measured. Measurement of R revealed that tissue samples were live, and their permeability to ions were stable. Control specimens had PD -0.22 mV (median). The PD of specimens treated by cisplatin was -0.55 mV (median), to for cisplatin and bumetanide 0 mV (median). Treatment with cisplatin did not change the continuous transport of Na+ and K+ ions, but did change that of Cl- ions. Stimulation of samples with the transport blockers of Cl-, Na+ and both induced repeatable and measurable reactions in the transport of the appropriate ions. It was shown that absorption of Na+ ions and release of Cl- ions was intensified than in the untreated specimens. It was proven in the study that cisplatin influences the Na+ and Cl- transport in the skin cells. Restoring the balance in ion flow can prevent side effects of use cisplatin-based drugs.
Postępy Higieny i Medycyny Doświadczalnej
N-acetyl-L-cysteine (NAC) mucolytic and antioxidant role is well known, but the effect on epithel... more N-acetyl-L-cysteine (NAC) mucolytic and antioxidant role is well known, but the effect on epithelial ion transport has not been yet described. The aim of the study was to evaluate the short-term and prolonged influence of NAC on ion transport in the epithelium. The experiment was performed on 108 fragments of rabbit tracheae. Fragments were divided into four groups: inhibited sodium (I) and chloride (II) transport, NAC with inhibited sodium (III) and NAC with inhibited chloride (IV) transport. The changes in electrophysiological parameters were measured in stationary conditions and during mechanical-chemical stimulation after immediate (15 s) and prolonged (60 min) N-acetylcysteine administration on the tissue. Each 15-second stimulation caused repeatable changes in the electric potential of the tissue. In trachea fragments with blocked chloride ion transport, significantly lower (P <0.0001) values of electric potential following prolonged NAC effect were observed when compared t...
Journal of Molecular Liquids
The Leeds Beckett repository holds a wide range of publications, each of which has been checked f... more The Leeds Beckett repository holds a wide range of publications, each of which has been checked for copyright and the relevant embargo period has been applied by the Research Services team. We operate on a standard take-down policy. If you are the author or publisher of an output and you would like it removed from the repository, please contact us and we will investigate on a case-by-case basis.
Advances in Dermatology and Allergology
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
In this study a new probe (2'-(1H-phenanthro[9,10-d]imidazol-2-yl)-phenyl-4-carboxylic acid N... more In this study a new probe (2'-(1H-phenanthro[9,10-d]imidazol-2-yl)-phenyl-4-carboxylic acid N-hydroxysuccinimide ester, PB1-1) was synthesized and presented, containing the ester group as reactive group for medical imaging applications. The tests included a comparison to the PB1 probe with the aldehyde group described earlier. Also, the photophysics of PB1 and PB1-1 when conjugated to albumin (HSA) and concanavalin A (Con A) was studied. The fluorescence anisotropy measurements and the method of fluorescence quenching of protein were used to examine these interactions. The results showed that both dyes are highly bound to the studied proteins, especially PB1-1. In the present study we also compared the stability of prepared conjugates. The in vitro study have shown that all tested compounds presented to be usable in the case of fixated cell staining. PB1-1-ConA and PB1-1-HSA were characterized with the lowest cytotoxicity during the MTT assay, and thus should be more suitable for live imaging applications than PB1-ConA and PB1-HSA. The results obtained in this work confirmed the theses presented in in silico studies as to the correctness of the choice of ester group as actively binding to the protein. At the same time, we have experimentally demonstrated the significant influence of a probe-protein linker on the spectral properties of conjugates used in medical imaging. We have clearly indicated that a detailed analysis of derivatives with different reactive group allows for proper probe selection. We also pointed out that based on the geometric skeleton of one dye, a whole range of fluorescent probes with different absorption and fluorescence spectra can be obtained for in vitro tests in medical imaging.
Pesticide Biochemistry and Physiology
Deltamethrin can be absorbed into the respiratory tract, the gastrointestinal tract and through t... more Deltamethrin can be absorbed into the respiratory tract, the gastrointestinal tract and through the skin. The study was designed to assess the effect of deltamethrin on electrophysiological parameters of rabbit's skin, studied in vitro, to identify the mechanism of action and effects of short-term dermal exposure to deltamethrin. The objective of the study was to investigate changes in electrophysiological parameters after exposure to 0.01 M deltamethrin under unchanged conditions, in the presence of amiloride (sodium transport blocker) and bumetanide (chloride transport blocker). Exposure to deltamethrin reduced the electrophysiological reaction of examined tissue in unchanged conditions and during the sodium reabsorption phase but did not influence the chloride ion secretion phase. The presented data show that the pyrethroide affects transepithelial ion transport in the external layers of the skin. The inhibition of chloride and sodium ions enabled evaluation of the impact of the pesticide on dermal transport.
Medicina
Background and Objectives: Top-level sports performance places heavy physical and psychological d... more Background and Objectives: Top-level sports performance places heavy physical and psychological demands on elite-level athletes, which can be a source of increased levels of stress. Therefore, high-level volleyball players may present altered cardiovascular and endocrinological stress response during stressful events. Although many previous studies have examined the response to stress on athletes, most of them regarded only males, while the impact of the female menstrual cycle has rarely been taken into account. We aimed to study psychophysiological response to anticipatory stressor through analysis of heart rate, self-reported anxiety level, and salivary cortisol in healthy young female athletes by minimalizing the effect of confounders. Materials and Methods: A total of 55 females (25 members of the best league for female volleyball players in Poland and 30 sedentary-lifestyle control subjects) in the follicular phase of their menstrual cycle were exposed to mental arithmetic task...
Biomedical Journal of Scientific & Technical Research
Postępy Higieny i Medycyny Doświadczalnej
Streszczenie Pyretroidy, syntetyczne pochodne naturalnych pyretryn pochodzących z Chrysanthemum c... more Streszczenie Pyretroidy, syntetyczne pochodne naturalnych pyretryn pochodzących z Chrysanthemum cinerariaefolim, powszechnie stosowane do ochrony roślin, w leśnictwie, przemyśle rolniczym, farmaceutycznym, a także w medycynie oraz weterynarii. Do organizmu mogą się dostać poprzez wdychanie, spożycie oraz kontakt ze skórą. Przyjęto, że charakteryzują się niewielką toksycznością dla ludzi, są szybko metabolizowane i nie kumulują się w tkankach, a wydalane są z moczem. Mimo istniejących licznych obostrzeń, ich stosowanie niesie duże ryzyko, ponieważ związki te oraz ich metabolity mogą przedostawać się do środowiska naturalnego, zanieczyszczając wodę, glebę oraz żywność. Od wielu lat opisywane są skutki stosowania pyretroidów jako bezpośrednie zagrożenie dla zdrowia zwierząt oraz ludzi. Na bieżąco publikowane są informuje o zatruciach tymi związkami u ludzi i zwierząt oraz o ofiarach śmiertelnych po ich zażyciu. Najbardziej narażone są dzieci, gdyż pyretroidy mogą występować w mleku mat...
Diagnostyka Laboratoryjna, Apr 13, 2018
Cortisol, also called “the” stress hormone is a glucocorticoid secreted by the adrenal cortex. Th... more Cortisol, also called “the” stress hormone is a glucocorticoid secreted by the adrenal cortex. This hormone plays a significant role in maintaining homeostasis, according to the body’s total stress. Cortisol interferes with many organs, affects glucose and fatty acids metabolism and neurotransmitter secretion. Predominantly, cortisol influences the carbohydrate metabolism, stimulating gluconeogenesis in the liver and inhibiting glucose utilization in peripheral tissues. As it is an element “fight or flight” it also stimulates central nervous system and enhances blood flow. To some extent cortisol influences also the renal handling of electrolytes, namely: increasing sodium resorption, and renal excretion of potassium, calcium and phosphates. Through its anti-inflammatory and immunosuppressive character this glucocorticoid modulates the immune system functioning. Cortisol has a circadian rhythm following ACTH (adrenocorticotropic hormone) secretion. Increased cortisol levels are observed physiologically during stress and pathologically in Cushing’s syndrome. Chronic hypercortisolism is harmful or the body, and its effects present an extremely wide spectrum, including insulin resistance, obesity, insomnia and even depression. Thus, laboratory diagnosis of cortisol level is important for the diagnosis, monitoring and evaluate the effectiveness of hypercortisolism treatment. Słowa kluczowe: hiperkortyzolemia, oznaczanie kortyzolu, reakcja stresowa;
Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego, Sep 29, 2017
Based on the analysis of data from clinical trials it could be postulated that N-acetylcysteine h... more Based on the analysis of data from clinical trials it could be postulated that N-acetylcysteine has a positive impact on the treatment of various diseases. However, less is known about specific molecular and physiological mechanisms underlying the reported therapeutic effects. N-acetylcysteine (NAC, N-acetyl-L-cysteine) is an amino acid derivative containing a thiol group. It is a precursor of L-cysteine and glutathione. NAC is well absorbed and safe for the body at doses up to 300 mg per kg of body weight. Side effects are relatively rare. NAC is used as an mucolytic agent in adjunctive therapy of respiratory diseases causing the retention of secretions, as well as an antidote in the treatment of paracetamol poisoning. Moreover, NAC protects against the toxic effects of reactive oxygen species and their active metabolites. NAC is involved in free radical scavenging processes via several independent mechanisms, including a direct reduction of free radicals, providing substrates for ...
Postȩpy higieny i medycyny doświadczalnej, 2021
Pyrethroids, synthetic derivatives of natural pyrethrins derived from Chrysanthemum cinerariaefol... more Pyrethroids, synthetic derivatives of natural pyrethrins derived from Chrysanthemum cinerariaefolim, are commonly used for plant protection in the forestry, agricultural, pharmaceutical industry as well as in medicine and veterinary medicine. They can enter the body by inhalation, ingestion and skin contact. It was assumed that they are characterized by low toxicity to humans, are quickly metabolized and do not accumulate in tissues, and are excreted in the urine. Despite the existing restrictions, their use carries a great risk, because these compounds and their metabolites can get into the natural environment, contaminating water, soil and food. The consequences of using pyrethroids as a direct threat to animal and human health have been described for many years. They are published on an ongoing basis informing about poisoning with these compounds in humans and animals, and about fatalities after their taking. Children are most at risk because pyrethroids can be found in breast mi...
Medicina
Pyrethroids are pesticides commonly used in crop protection; in the forestry, wood, and textile i... more Pyrethroids are pesticides commonly used in crop protection; in the forestry, wood, and textile industries; as well as in medicine and veterinary medicine to treat parasitic crustacean infestations. They have been found to be relatively safe for humans and animals. Pyrethroids are recommended for personal protection against malaria and virus Zika by the World Health Organization. Pyrethroids act on voltage-gated sodium channels, which cause an influx of sodium ions into the nerve cells and permanent depolarization. They also influence activities of enzymes, especially in nerve and liver cells. Contact of pyrethroids with the skin, digestive tract, and respiratory tract results in their penetration into the body. Due to the importance of the subject, a summary of the current state of knowledge on the toxic effects of pyrethroids was presented in the comprehensive review by Chrustek et al, published in journal Medicina. Particular attention was paid to nephrotoxic, hepatotoxic, cardio...
Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego, 2017
Based on the analysis of data from clinical trials it could be postulated that N-acetylcysteine h... more Based on the analysis of data from clinical trials it could be postulated that N-acetylcysteine has a positive impact on the treatment of various diseases. However, less is known about specific molecular and physiological mechanisms underlying the reported therapeutic effects. N-acetylcysteine (NAC, N-acetyl-L-cysteine) is an amino acid derivative containing a thiol group. It is a precursor of L-cysteine and glutathione. NAC is well absorbed and safe for the body at doses up to 300 mg per kg of body weight. Side effects are relatively rare. NAC is used as an mucolytic agent in adjunctive therapy of respiratory diseases causing the retention of secretions, as well as an antidote in the treatment of paracetamol poisoning. Moreover, NAC protects against the toxic effects of reactive oxygen species and their active metabolites. NAC is involved in free radical scavenging processes via several independent mechanisms, including a direct reduction of free radicals, providing substrates for ...
Pyrethroids are pesticides commonly used in crop protection; in the forestry, wood, and textile i... more Pyrethroids are pesticides commonly used in crop protection; in the forestry, wood, and textile industries; as well as in medicine and veterinary medicine to treat parasitic crustacean infestations. They have been found to be relatively safe for humans and animals. Pyrethroids are recommended for personal protection against malaria and virus Zika by the World Health Organization. Pyrethroids act on voltage-gated sodium channels, which cause an influx of sodium ions into the nerve cells and permanent depolarization. They also influence activities of enzymes, especially in nerve and liver cells. Contact of pyrethroids with the skin, digestive tract, and respiratory tract results in their penetration into the body. Due to the importance of the subject, a summary of the current state of knowledge on the toxic effects of pyrethroids was presented in the comprehensive review by Chrustek et al, published in journal Medicina. Particular attention was paid to nephrotoxic, hepatotoxic, cardio...
Cancers
Ovarian cancer is one of the most common malignancies among women worldwide. The course of the di... more Ovarian cancer is one of the most common malignancies among women worldwide. The course of the disease is often latent and asymptomatic in the early stages, but as it develops, metastasis occurs, accompanied by accumulation of ascites in the peritoneal cavity. The ascites fluid constitutes a specific microenvironment influencing the processes of carcinogenesis. In ascites, signaling is mediated by various cytokines that control tumor cell proliferation, progression, metastasis, and chemoresistance. Adipokines, secreted into ascites and also appearing in blood, may be markers of ongoing processes related to the development of neoplastic disease. Moreover, a significant influence of adipocyte lipids on the growth of tumors, for which they are one of energy sources, is observed. Adiponectin, interleukin 6 (IL-6), interleukin 8 (IL-8), monocyte chemotactic protein-1 (MCP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1), discussed in the present review, were found to mediate the e...
PLOS ONE
The preservation of physiological transport of ions and water content is particularly important f... more The preservation of physiological transport of ions and water content is particularly important for maintaining the skin barrier, touch and pain stimuli, as well as the initiation of skin regeneration processes, especially after treatments associated with breaking skin continuity and wound healing difficulties. The aim of the study was to assess changes in ion transport, measured as values of transepithelial electric resistance and potential difference in stationary conditions and during mechanical-chemical stimulations, depending on the hydration status of isolated rabbit skin specimens. The specimens were divided into five groups: control (n = 22), dehydrated in 10% NaCl (n = 30), rehydrated after dehydration (n = 26), dried at 37°C (n = 26), and rehydrated after drying (n = 25). Dehydrated tissue samples showed altered resistance compared to the control; this change was maintained regardless of rehydration. In the dehydrated samples, changes in the measured electric potential wer...
Scientific Reports
The measurement of electric potential and resistance reflect the transport of sodium and chloride... more The measurement of electric potential and resistance reflect the transport of sodium and chloride ions which take place in keratinocytes and is associated with skin response to stimuli arising from external and internal environment. The aim of the study was to assess changes in electrical resistance and the transport of chloride and sodium ions, under iso-osmotic conditions and following the use of inhibitors affecting these ions’ transport, namely amiloride (A) and bumetanide (B). The experiment was performed on 104 fragments of rabbit skin, divided into three groups: control (n = 35), A—inhibited sodium transport (n = 33) and B—inhibited chloride transport (n = 36). Measurement of electrical resistance (R) and electrical potential (PD) confirmed tissue viability during the experiment, no statistically significant differences in relation to control conditions were noted. The minimal and maximal PD measured during stimulation confirmed the repeatability of the recorded reactions to ...
Skin Pharmacology and Physiology
Acute, adverse skin effects to capsaicin can be activated by inhibition of sodium transport not o... more Acute, adverse skin effects to capsaicin can be activated by inhibition of sodium transport not only in nociceptive neurons, but also in keratinocytes. The aim of the current study was to describe and compare immediate (15 s) and prolonged (30 min) effects of capsaicin on epidermal (not neural) sodium transport using a rabbit skin model. Skin fragments (n = 169) were incubated in 4 conditions: undisturbed ion transport (U; n = 48); inhibited sodium transport (INa; n = 34) with amiloride used as sodium transport blocker; long-term irritation by capsaicin with undisturbed ion transport (CAPSA-U; n = 43) and with inhibited sodium transport (CAPSA-INa; n = 35). After 30 min of incubation, a solution of capsaicin was applied directly to the skin fragments. The study demonstrated that sodium transport inhibition eliminated the effects of both immediate and prolonged capsaicin application. The results could be the basis for future research considering selective sodium transport inhibitors for human skin to reduce the side effects of capsaicin, related to activation of sodium channels in keratinocytes.
Biomedicine & Pharmacotherapy
Platinum-based drugs, used in treating tumors, cause numerous undesirable effects in patients, li... more Platinum-based drugs, used in treating tumors, cause numerous undesirable effects in patients, like neuropathic pain, hypersensitivity, reddening, pruritus and rash. Changes in Na+ transport modify local osmolality and contribute to the initiation of hypersensitivity and allergy. They are also associated with stimulation of C-fibres and hyperalgesia. Cl- transport is essential for regulation of sweat composition and the migration of immunocompetent cells. The aim of the conducted study was to assess the effect of a cisplatin solution on the electrophysiological parameters of the isolated rabbit skin specimens. The difference in transepithelial electrical potential (PD) and resistance (R) in stationary conditions and during 15 s mechanical-chemical stimulation (PDmin and PDmax), were measured. Measurement of R revealed that tissue samples were live, and their permeability to ions were stable. Control specimens had PD -0.22 mV (median). The PD of specimens treated by cisplatin was -0.55 mV (median), to for cisplatin and bumetanide 0 mV (median). Treatment with cisplatin did not change the continuous transport of Na+ and K+ ions, but did change that of Cl- ions. Stimulation of samples with the transport blockers of Cl-, Na+ and both induced repeatable and measurable reactions in the transport of the appropriate ions. It was shown that absorption of Na+ ions and release of Cl- ions was intensified than in the untreated specimens. It was proven in the study that cisplatin influences the Na+ and Cl- transport in the skin cells. Restoring the balance in ion flow can prevent side effects of use cisplatin-based drugs.
Postępy Higieny i Medycyny Doświadczalnej
N-acetyl-L-cysteine (NAC) mucolytic and antioxidant role is well known, but the effect on epithel... more N-acetyl-L-cysteine (NAC) mucolytic and antioxidant role is well known, but the effect on epithelial ion transport has not been yet described. The aim of the study was to evaluate the short-term and prolonged influence of NAC on ion transport in the epithelium. The experiment was performed on 108 fragments of rabbit tracheae. Fragments were divided into four groups: inhibited sodium (I) and chloride (II) transport, NAC with inhibited sodium (III) and NAC with inhibited chloride (IV) transport. The changes in electrophysiological parameters were measured in stationary conditions and during mechanical-chemical stimulation after immediate (15 s) and prolonged (60 min) N-acetylcysteine administration on the tissue. Each 15-second stimulation caused repeatable changes in the electric potential of the tissue. In trachea fragments with blocked chloride ion transport, significantly lower (P <0.0001) values of electric potential following prolonged NAC effect were observed when compared t...
Journal of Molecular Liquids
The Leeds Beckett repository holds a wide range of publications, each of which has been checked f... more The Leeds Beckett repository holds a wide range of publications, each of which has been checked for copyright and the relevant embargo period has been applied by the Research Services team. We operate on a standard take-down policy. If you are the author or publisher of an output and you would like it removed from the repository, please contact us and we will investigate on a case-by-case basis.
Advances in Dermatology and Allergology
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
In this study a new probe (2'-(1H-phenanthro[9,10-d]imidazol-2-yl)-phenyl-4-carboxylic acid N... more In this study a new probe (2'-(1H-phenanthro[9,10-d]imidazol-2-yl)-phenyl-4-carboxylic acid N-hydroxysuccinimide ester, PB1-1) was synthesized and presented, containing the ester group as reactive group for medical imaging applications. The tests included a comparison to the PB1 probe with the aldehyde group described earlier. Also, the photophysics of PB1 and PB1-1 when conjugated to albumin (HSA) and concanavalin A (Con A) was studied. The fluorescence anisotropy measurements and the method of fluorescence quenching of protein were used to examine these interactions. The results showed that both dyes are highly bound to the studied proteins, especially PB1-1. In the present study we also compared the stability of prepared conjugates. The in vitro study have shown that all tested compounds presented to be usable in the case of fixated cell staining. PB1-1-ConA and PB1-1-HSA were characterized with the lowest cytotoxicity during the MTT assay, and thus should be more suitable for live imaging applications than PB1-ConA and PB1-HSA. The results obtained in this work confirmed the theses presented in in silico studies as to the correctness of the choice of ester group as actively binding to the protein. At the same time, we have experimentally demonstrated the significant influence of a probe-protein linker on the spectral properties of conjugates used in medical imaging. We have clearly indicated that a detailed analysis of derivatives with different reactive group allows for proper probe selection. We also pointed out that based on the geometric skeleton of one dye, a whole range of fluorescent probes with different absorption and fluorescence spectra can be obtained for in vitro tests in medical imaging.
Pesticide Biochemistry and Physiology
Deltamethrin can be absorbed into the respiratory tract, the gastrointestinal tract and through t... more Deltamethrin can be absorbed into the respiratory tract, the gastrointestinal tract and through the skin. The study was designed to assess the effect of deltamethrin on electrophysiological parameters of rabbit's skin, studied in vitro, to identify the mechanism of action and effects of short-term dermal exposure to deltamethrin. The objective of the study was to investigate changes in electrophysiological parameters after exposure to 0.01 M deltamethrin under unchanged conditions, in the presence of amiloride (sodium transport blocker) and bumetanide (chloride transport blocker). Exposure to deltamethrin reduced the electrophysiological reaction of examined tissue in unchanged conditions and during the sodium reabsorption phase but did not influence the chloride ion secretion phase. The presented data show that the pyrethroide affects transepithelial ion transport in the external layers of the skin. The inhibition of chloride and sodium ions enabled evaluation of the impact of the pesticide on dermal transport.
Medicina
Background and Objectives: Top-level sports performance places heavy physical and psychological d... more Background and Objectives: Top-level sports performance places heavy physical and psychological demands on elite-level athletes, which can be a source of increased levels of stress. Therefore, high-level volleyball players may present altered cardiovascular and endocrinological stress response during stressful events. Although many previous studies have examined the response to stress on athletes, most of them regarded only males, while the impact of the female menstrual cycle has rarely been taken into account. We aimed to study psychophysiological response to anticipatory stressor through analysis of heart rate, self-reported anxiety level, and salivary cortisol in healthy young female athletes by minimalizing the effect of confounders. Materials and Methods: A total of 55 females (25 members of the best league for female volleyball players in Poland and 30 sedentary-lifestyle control subjects) in the follicular phase of their menstrual cycle were exposed to mental arithmetic task...
Biomedical Journal of Scientific & Technical Research