Igor Dziecielewski - Academia.edu (original) (raw)
Papers by Igor Dziecielewski
Physical Review B, 2011
... Marta Sawicka,1,2,* Henryk Turski,1 Marcin Siekacz,1,2 Julita Smalc-Koziorowska,1,2,3 Marcin ... more ... Marta Sawicka,1,2,* Henryk Turski,1 Marcin Siekacz,1,2 Julita Smalc-Koziorowska,1,2,3 Marcin Krysko,1 Igor Dzi˛ecielewski,1 Izabella Grzegory,1 and Czesław Skierbiszewski1,2 1Institute of High Pressure Physics, Polish Academy of Sciences, Sokołowska 29/37, PL-01-142 ...
This article describes the detection of DNA mutations using novel Au-Ag coated GaN substrate as S... more This article describes the detection of DNA mutations using novel Au-Ag coated GaN substrate as SERS (surface-enhanced Raman spectroscopy) diagnostic platform. Oligonucleotide sequences corresponding to the BCR-ABL (breakpoint cluster region-Abelson) gene responsible for development of chronic myelogenous leukemia were used as a model system to demonstrate the discrimination between the wild type and Met244Val mutations. The thiolated ssDNA (single-strand DNA) was immobilized on the SERS-active surface and then hybridized to a labeled target sequence from solution. An intense SERS signal of the reporter molecule MGITC was detected from the complementary target due to formation of double helix. The SERS signal was either not observed, or decreased dramatically for a negative control sample consisting of labeled DNA that was not complementary to the DNA probe. The results indicate that our SERS substrate offers an opportunity for the development of novel diagnostic assays.
ACS Applied Materials & Interfaces, 2015
A novel inorganic nanocomposite material, called BOA, which has the form of small building blocks... more A novel inorganic nanocomposite material, called BOA, which has the form of small building blocks composed of gold nanoparticles embedded in a polyoxoborate matrix, is presented. It is demonstrated that cotton wool decorated with the BOA nanocomposite displays strong antibacterial activity toward both Gram-positive and -negative bacteria strains. Importantly, the modified cotton does not release any toxic substances, and the bacteria are killed upon contact with the fibers coated with the BOA. Toxicity tests show that the nanocomposite-in spite of its antiseptic properties-is harmless for mammalian cells. The presented method of surface modification utilizes mild, environmentally friendly fabrication conditions. Thus, it offers a facile approach to obtain durable nontoxic antiseptic coatings for biomedical applications.
Biosensors and Bioelectronics, 2015
A highly sensitive immunoassay utilizing surface-enhanced Raman scattering (SERS) has been develo... more A highly sensitive immunoassay utilizing surface-enhanced Raman scattering (SERS) has been developed with a new Raman reporter and a unique SERS-active substrate incorporated into a microfluidic device. An appropriately designed Raman reporter, basic fuchsin (FC), gives strong SERS enhancement and has the ability to bind both the antibody and gold nanostructures. The fuchsin-labeled immuno-Au nanoflowers can form a sandwich structure with the antigen and the antibody immobilized on the SERS-active substrate based on Au-Ag coated GaN. Our experimental results indicate that this SERS-active substrate with its strong surface-enhancement factor, high stability and reproducibility plays a crucial role in improving the efficiency of SERS immunoassay. This SERS assay was applied to the detection of Hepatitis B virus antigen (HBsAg) in human blood plasma. A calibration curve was obtained by plotting the intensity of SERS signal of FC band at 1178cm(-1) versus the concentration of antigen. The low detection limit for Hepatitis B virus antigen was estimated to be 0.01IU/mL. The average relative standard deviation (RSD) of this method is less than 10%. This SERS immunoassay gives exact results over a broad linear range, reflecting clinically relevant HBsAg concentrations. It also exhibits high biological specificity for the detection of Hepatitis B virus antigen.
physica status solidi (c), 2011
Abstract It is suggested that final bowing of {0001} planes in free standing GaN depends mostly o... more Abstract It is suggested that final bowing of {0001} planes in free standing GaN depends mostly on the nucleation mechanism at the beginning of the growth in the presented approach. Typical growth process in this study, includes formation of pyramidal islands ...
physica status solidi (a), 2011
ABSTRACT In this paper the growth and physical properties of HNPS-GaN crystallized in “feed-seed”... more ABSTRACT In this paper the growth and physical properties of HNPS-GaN crystallized in “feed-seed” configuration is described. The idea of this configuration is based on the conversion of the free standing HVPE-GaN crystals to the free standing HNPS-GaN. The influence of the c-plane bowing in the initial substrate on quality, rate, and mode of growth from solution is analyzed.
Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures, 2011
ABSTRACT Homoepitaxial growth of m -plane GaN (1 1 00) as a function of substrate miscut and temp... more ABSTRACT Homoepitaxial growth of m -plane GaN (1 1 00) as a function of substrate miscut and temperature was studied by plasma assisted molecular beam epitaxy (PAMBE). The authors demonstrate that it is possible to obtain high-quality GaN on the m -plane under nitrogen-rich conditions at 730 ° C . This is in contrast to the c -plane where three-dimensional growth mode is observed under the same conditions. They find a strong growth anisotropy and describe GaN (1 1 00) surface morphology dependence on the sample miscut direction. The results indicate that by introducing a sample miscut toward <11 2 6 > one may expect parallel atomic steps when growing under nitrogen-rich conditions at 730 ° C by PAMBE.
Journal of Nanoparticle Research, 2012
Controlled patterning and formation of nanostructures on surfaces based on self-assembly is a pro... more Controlled patterning and formation of nanostructures on surfaces based on self-assembly is a promising area in the field of ''bottom-up'' nanomaterial engineering. We report formation of net-like structures of gold nanoparticles (Au NPs) in a matrix of liquid crystalline amphiphile 4 0 -n-octyl-4-cyanobiphenyl at the air-water interface. After initial compression to at least 18 mN m -1 , decompression of a Langmuir film of a mixture containing both components results in formation of net-like structures. The average size of a unit cell of the net is easily adjustable by changing the surface pressure during the decompression of the film. The net-like patterns of different, desired average unit cell areas were transferred onto solid substrates (Langmuir-Blodgett method) and investigated with scanning electron microscopy and X-ray reflectivity (XRR). Uniform coverage over large areas was proved. XRR data revealed lifting of the Au NPs from the surface during the formation of the film. A molecular mechanism of formation of the net-like structures is discussed.
Journal of Materials Chemistry, 2011
We fabricated a Surface Enhanced Raman Scattering (SERS)-active surface based on photo-etched and... more We fabricated a Surface Enhanced Raman Scattering (SERS)-active surface based on photo-etched and Au-coated GaN. The highest enhancement factor (EF) in SERS and high reproducibility of spectra were obtained from surfaces covered with bunched nanopillars which were produced by relatively long defect-selective photo-etching. The surfaces exhibited SERS enhancements of the order of 2.8 Â 10 6 for malachite green isothiocyanate (MGITC) and 2 Â 10 6 for p-mercaptobenzoic acid (PMBA). These SERS enhancement factors were comparable to those of conventional SERS substrates, while the EF for MGITC was two orders of magnitude larger than the corresponding one reported for the SERS platform made on porous GaN. The standard deviation of the relative intensity of the 1180 cm À1 mode of MGITC was less than 5% for 100 randomly distributed locations across a single platform and less than 10% between different platforms. The SERS signal of MGITC at our GaN/Au surface (kept under ambient conditions) was extremely stable. We could not detect any peak shift or appreciable change of intensity even after three months. We used these surfaces to detect biological molecules such as amino acids and bovine serum albumin (BSA) at low concentration and with short detection time. We developed simple and effective cleaning procedures for our substrates. After cleaning, the same substrate could be used multiple times retaining the SERS activity. We are not aware of any other multiply regenerated SERS substrate which provides simultaneously such high stability with high enhancement, good uniformity, and high reproducibility.
Journal of Crystal Growth, 2013
ABSTRACT An influence of a nitridation process on a self lift-off phenomenon during the HVPE crys... more ABSTRACT An influence of a nitridation process on a self lift-off phenomenon during the HVPE crystallization of GaN on a MOCVD-GaN/sapphire template with photolitographically patterned Ti mask was investigated. Duration of the nitridation process and flows of reagents in this process were modified. A correlation between degradation degree of a GaN template surface and the conditions of the nitridation process was observed. It was also observed that the degree of degradation had a direct influence on a moment of the self lift-off phenomenon (separation of a HVPE-GaN crystal from the template). It was shown that the best free-standing HVPE-GaN crystals, in sense of their structural quality, were obtained when the separation process occurred during cooling down step.
Journal of Applied Physics, 2012
ABSTRACT The fabrication procedure of efficient surface enhanced Raman scattering (SERS) platform... more ABSTRACT The fabrication procedure of efficient surface enhanced Raman scattering (SERS) platforms is demonstrated based on reproducibly photo-etched GaN epitaxial layers covered by a thin Au-Ag layer and subjected to partial de-alloying. Using a gold-rich Au57Ag43 alloy (70/30 wt. %), it is possible to preserve about 19 at. % of silver in the layer, even after etching in nitric acid for up to 24 h. A large enhancement of the Raman signal from the test 4-mercaptobenzoic acid molecules attached to such porous Au-Ag metal layer is obtained due to the presence of a high percentage of Ag. For the optimal etching time, in the range of 6–24 h, an enhancement factor (EF) greater than 107 was obtained. The contribution of different features of our SERS platforms such as the size of pores, nano-roughness, and the chemical composition of the metal layer for the EF parameter are critically discussed.
Applied Physics Letters, 2013
Engineering the composition, morphology, and optical properties of InAsSb nanostructures via grad... more Engineering the composition, morphology, and optical properties of InAsSb nanostructures via graded growth technique Appl. Phys. Lett. 102, 033111 High excitation carrier density recombination dynamics of InGaN/GaN quantum well structures: Possible relevance to efficiency droop Appl. Phys. Lett. 102, 022106 (2013) Additional information on Appl. Phys. Lett.
Chemistry of Materials, 2012
ABSTRACT We report an innovative application of a true “bottom-up” approach for preparation of fu... more ABSTRACT We report an innovative application of a true “bottom-up” approach for preparation of functional material. Three consecutive self-assembly steps were utilized for formation of a complex surface enhanced Raman spectroscopy (SERS) platform. First the Langmuir–Blodgett technique was used to deposit gold nanoparticles on a solid substrate. Thus prepared surfaces were afterward used as substrates in the chemical vapor deposition process of gallium nitride (GaN) nanowire growth. On such scaffolding, a third step of material fabrication was performed. Gold microflowers (Au MFs) deposited from solution preferably appeared at the top of the GaN nanowires and not in the cavities in between. The obtained morphology of the final material was controlled at each step of the preparation process to tailor its properties for desired purposes. Prepared surfaces were tested as SERS platforms. The enhancement factor was around 107 in case of p-mercaptobenzoic acid (p-MBA). The platforms were also suitable for biological and biomedical applications. We demonstrated the label free detection of DNA. The substrates gave reproducible SERS spectra both across a single platform and between different platforms. The average spectral correlation coefficients (Γ) was 0.87. Moreover, the obtained material proved to be very stable. The presented complex structure demonstrated therefore had the advantages of the two surface functionalization concepts it comprised: (1) GaN nanowire growth and (2) Au MF deposition, eliminating their major drawbacks. Presented material combined high SERS enhancement factor of Au MFs deposited on a flat surface and good durability of microflowers deposited on a surface completely covered with nanowires, which were almost SERS inactive. The final product provided truly exceptional stability and repeatability of SERS results, maintaining an enhancement factor comparable to the best commercially available platforms.
Physical Review B, 2011
... Marta Sawicka,1,2,* Henryk Turski,1 Marcin Siekacz,1,2 Julita Smalc-Koziorowska,1,2,3 Marcin ... more ... Marta Sawicka,1,2,* Henryk Turski,1 Marcin Siekacz,1,2 Julita Smalc-Koziorowska,1,2,3 Marcin Krysko,1 Igor Dzi˛ecielewski,1 Izabella Grzegory,1 and Czesław Skierbiszewski1,2 1Institute of High Pressure Physics, Polish Academy of Sciences, Sokołowska 29/37, PL-01-142 ...
This article describes the detection of DNA mutations using novel Au-Ag coated GaN substrate as S... more This article describes the detection of DNA mutations using novel Au-Ag coated GaN substrate as SERS (surface-enhanced Raman spectroscopy) diagnostic platform. Oligonucleotide sequences corresponding to the BCR-ABL (breakpoint cluster region-Abelson) gene responsible for development of chronic myelogenous leukemia were used as a model system to demonstrate the discrimination between the wild type and Met244Val mutations. The thiolated ssDNA (single-strand DNA) was immobilized on the SERS-active surface and then hybridized to a labeled target sequence from solution. An intense SERS signal of the reporter molecule MGITC was detected from the complementary target due to formation of double helix. The SERS signal was either not observed, or decreased dramatically for a negative control sample consisting of labeled DNA that was not complementary to the DNA probe. The results indicate that our SERS substrate offers an opportunity for the development of novel diagnostic assays.
ACS Applied Materials & Interfaces, 2015
A novel inorganic nanocomposite material, called BOA, which has the form of small building blocks... more A novel inorganic nanocomposite material, called BOA, which has the form of small building blocks composed of gold nanoparticles embedded in a polyoxoborate matrix, is presented. It is demonstrated that cotton wool decorated with the BOA nanocomposite displays strong antibacterial activity toward both Gram-positive and -negative bacteria strains. Importantly, the modified cotton does not release any toxic substances, and the bacteria are killed upon contact with the fibers coated with the BOA. Toxicity tests show that the nanocomposite-in spite of its antiseptic properties-is harmless for mammalian cells. The presented method of surface modification utilizes mild, environmentally friendly fabrication conditions. Thus, it offers a facile approach to obtain durable nontoxic antiseptic coatings for biomedical applications.
Biosensors and Bioelectronics, 2015
A highly sensitive immunoassay utilizing surface-enhanced Raman scattering (SERS) has been develo... more A highly sensitive immunoassay utilizing surface-enhanced Raman scattering (SERS) has been developed with a new Raman reporter and a unique SERS-active substrate incorporated into a microfluidic device. An appropriately designed Raman reporter, basic fuchsin (FC), gives strong SERS enhancement and has the ability to bind both the antibody and gold nanostructures. The fuchsin-labeled immuno-Au nanoflowers can form a sandwich structure with the antigen and the antibody immobilized on the SERS-active substrate based on Au-Ag coated GaN. Our experimental results indicate that this SERS-active substrate with its strong surface-enhancement factor, high stability and reproducibility plays a crucial role in improving the efficiency of SERS immunoassay. This SERS assay was applied to the detection of Hepatitis B virus antigen (HBsAg) in human blood plasma. A calibration curve was obtained by plotting the intensity of SERS signal of FC band at 1178cm(-1) versus the concentration of antigen. The low detection limit for Hepatitis B virus antigen was estimated to be 0.01IU/mL. The average relative standard deviation (RSD) of this method is less than 10%. This SERS immunoassay gives exact results over a broad linear range, reflecting clinically relevant HBsAg concentrations. It also exhibits high biological specificity for the detection of Hepatitis B virus antigen.
physica status solidi (c), 2011
Abstract It is suggested that final bowing of {0001} planes in free standing GaN depends mostly o... more Abstract It is suggested that final bowing of {0001} planes in free standing GaN depends mostly on the nucleation mechanism at the beginning of the growth in the presented approach. Typical growth process in this study, includes formation of pyramidal islands ...
physica status solidi (a), 2011
ABSTRACT In this paper the growth and physical properties of HNPS-GaN crystallized in “feed-seed”... more ABSTRACT In this paper the growth and physical properties of HNPS-GaN crystallized in “feed-seed” configuration is described. The idea of this configuration is based on the conversion of the free standing HVPE-GaN crystals to the free standing HNPS-GaN. The influence of the c-plane bowing in the initial substrate on quality, rate, and mode of growth from solution is analyzed.
Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures, 2011
ABSTRACT Homoepitaxial growth of m -plane GaN (1 1 00) as a function of substrate miscut and temp... more ABSTRACT Homoepitaxial growth of m -plane GaN (1 1 00) as a function of substrate miscut and temperature was studied by plasma assisted molecular beam epitaxy (PAMBE). The authors demonstrate that it is possible to obtain high-quality GaN on the m -plane under nitrogen-rich conditions at 730 ° C . This is in contrast to the c -plane where three-dimensional growth mode is observed under the same conditions. They find a strong growth anisotropy and describe GaN (1 1 00) surface morphology dependence on the sample miscut direction. The results indicate that by introducing a sample miscut toward <11 2 6 > one may expect parallel atomic steps when growing under nitrogen-rich conditions at 730 ° C by PAMBE.
Journal of Nanoparticle Research, 2012
Controlled patterning and formation of nanostructures on surfaces based on self-assembly is a pro... more Controlled patterning and formation of nanostructures on surfaces based on self-assembly is a promising area in the field of ''bottom-up'' nanomaterial engineering. We report formation of net-like structures of gold nanoparticles (Au NPs) in a matrix of liquid crystalline amphiphile 4 0 -n-octyl-4-cyanobiphenyl at the air-water interface. After initial compression to at least 18 mN m -1 , decompression of a Langmuir film of a mixture containing both components results in formation of net-like structures. The average size of a unit cell of the net is easily adjustable by changing the surface pressure during the decompression of the film. The net-like patterns of different, desired average unit cell areas were transferred onto solid substrates (Langmuir-Blodgett method) and investigated with scanning electron microscopy and X-ray reflectivity (XRR). Uniform coverage over large areas was proved. XRR data revealed lifting of the Au NPs from the surface during the formation of the film. A molecular mechanism of formation of the net-like structures is discussed.
Journal of Materials Chemistry, 2011
We fabricated a Surface Enhanced Raman Scattering (SERS)-active surface based on photo-etched and... more We fabricated a Surface Enhanced Raman Scattering (SERS)-active surface based on photo-etched and Au-coated GaN. The highest enhancement factor (EF) in SERS and high reproducibility of spectra were obtained from surfaces covered with bunched nanopillars which were produced by relatively long defect-selective photo-etching. The surfaces exhibited SERS enhancements of the order of 2.8 Â 10 6 for malachite green isothiocyanate (MGITC) and 2 Â 10 6 for p-mercaptobenzoic acid (PMBA). These SERS enhancement factors were comparable to those of conventional SERS substrates, while the EF for MGITC was two orders of magnitude larger than the corresponding one reported for the SERS platform made on porous GaN. The standard deviation of the relative intensity of the 1180 cm À1 mode of MGITC was less than 5% for 100 randomly distributed locations across a single platform and less than 10% between different platforms. The SERS signal of MGITC at our GaN/Au surface (kept under ambient conditions) was extremely stable. We could not detect any peak shift or appreciable change of intensity even after three months. We used these surfaces to detect biological molecules such as amino acids and bovine serum albumin (BSA) at low concentration and with short detection time. We developed simple and effective cleaning procedures for our substrates. After cleaning, the same substrate could be used multiple times retaining the SERS activity. We are not aware of any other multiply regenerated SERS substrate which provides simultaneously such high stability with high enhancement, good uniformity, and high reproducibility.
Journal of Crystal Growth, 2013
ABSTRACT An influence of a nitridation process on a self lift-off phenomenon during the HVPE crys... more ABSTRACT An influence of a nitridation process on a self lift-off phenomenon during the HVPE crystallization of GaN on a MOCVD-GaN/sapphire template with photolitographically patterned Ti mask was investigated. Duration of the nitridation process and flows of reagents in this process were modified. A correlation between degradation degree of a GaN template surface and the conditions of the nitridation process was observed. It was also observed that the degree of degradation had a direct influence on a moment of the self lift-off phenomenon (separation of a HVPE-GaN crystal from the template). It was shown that the best free-standing HVPE-GaN crystals, in sense of their structural quality, were obtained when the separation process occurred during cooling down step.
Journal of Applied Physics, 2012
ABSTRACT The fabrication procedure of efficient surface enhanced Raman scattering (SERS) platform... more ABSTRACT The fabrication procedure of efficient surface enhanced Raman scattering (SERS) platforms is demonstrated based on reproducibly photo-etched GaN epitaxial layers covered by a thin Au-Ag layer and subjected to partial de-alloying. Using a gold-rich Au57Ag43 alloy (70/30 wt. %), it is possible to preserve about 19 at. % of silver in the layer, even after etching in nitric acid for up to 24 h. A large enhancement of the Raman signal from the test 4-mercaptobenzoic acid molecules attached to such porous Au-Ag metal layer is obtained due to the presence of a high percentage of Ag. For the optimal etching time, in the range of 6–24 h, an enhancement factor (EF) greater than 107 was obtained. The contribution of different features of our SERS platforms such as the size of pores, nano-roughness, and the chemical composition of the metal layer for the EF parameter are critically discussed.
Applied Physics Letters, 2013
Engineering the composition, morphology, and optical properties of InAsSb nanostructures via grad... more Engineering the composition, morphology, and optical properties of InAsSb nanostructures via graded growth technique Appl. Phys. Lett. 102, 033111 High excitation carrier density recombination dynamics of InGaN/GaN quantum well structures: Possible relevance to efficiency droop Appl. Phys. Lett. 102, 022106 (2013) Additional information on Appl. Phys. Lett.
Chemistry of Materials, 2012
ABSTRACT We report an innovative application of a true “bottom-up” approach for preparation of fu... more ABSTRACT We report an innovative application of a true “bottom-up” approach for preparation of functional material. Three consecutive self-assembly steps were utilized for formation of a complex surface enhanced Raman spectroscopy (SERS) platform. First the Langmuir–Blodgett technique was used to deposit gold nanoparticles on a solid substrate. Thus prepared surfaces were afterward used as substrates in the chemical vapor deposition process of gallium nitride (GaN) nanowire growth. On such scaffolding, a third step of material fabrication was performed. Gold microflowers (Au MFs) deposited from solution preferably appeared at the top of the GaN nanowires and not in the cavities in between. The obtained morphology of the final material was controlled at each step of the preparation process to tailor its properties for desired purposes. Prepared surfaces were tested as SERS platforms. The enhancement factor was around 107 in case of p-mercaptobenzoic acid (p-MBA). The platforms were also suitable for biological and biomedical applications. We demonstrated the label free detection of DNA. The substrates gave reproducible SERS spectra both across a single platform and between different platforms. The average spectral correlation coefficients (Γ) was 0.87. Moreover, the obtained material proved to be very stable. The presented complex structure demonstrated therefore had the advantages of the two surface functionalization concepts it comprised: (1) GaN nanowire growth and (2) Au MF deposition, eliminating their major drawbacks. Presented material combined high SERS enhancement factor of Au MFs deposited on a flat surface and good durability of microflowers deposited on a surface completely covered with nanowires, which were almost SERS inactive. The final product provided truly exceptional stability and repeatability of SERS results, maintaining an enhancement factor comparable to the best commercially available platforms.