Ilaria Altieri - Academia.edu (original) (raw)

Papers by Ilaria Altieri

Journal of Chromatography B: Biomedical Sciences and Applications, Jul 1, 1991

Reproductive Toxicology, 2018

Low dose exposure to HBCD, CB-153 or TCDD induces histopathological and hormonal effects and chan... more Low dose exposure to HBCD, CB-153 or TCDD induces histopathological and hormonal effects and changes in brain protein and gene expression in juvenile female BALB/c mice. Reproductive Toxicology.

Journal of Chromatography B: Biomedical Sciences and Applications, Feb 1, 1995

A rapid and selective assay of morphine and its 3- and 6-glucuronides in serum, based on high-per... more A rapid and selective assay of morphine and its 3- and 6-glucuronides in serum, based on high-performance liquid chromatography-electrospray mass spectrometry has been developed. The analytes and the internal standard, codeine or naltrexone, were subjected to solid-phase extraction, using ethyl solid-phase extraction columns, prior to chromatography. A reversed-phase column and a gradient mobile phase consisting of water and methanol were used. The mass spectrometer was operated in the selected-ion monitoring mode. The following ions were used: m/z 286 for morphine, m/z 300 for codeine, m/z 342 for naltrexone, and m/z 462 for morphine 3- and 6-glucuronides. The limit of quantitation observed with this method was 10 ng/ml morphine, 50 ng/ml morphine-6-glucuronide and 100 ng/ml morphine-3-glucuronide. The present method proved useful for the determination of serum levels of the parent drug and its metabolites in pain patients, heroin addicts and in morphine-treated mice.

Food Chemistry, 2016

The evaluation of histamine content in fish and fishery products, responsible for scombroid poiso... more The evaluation of histamine content in fish and fishery products, responsible for scombroid poisoning, is essential to guarantee the safety of food. EU regulation requires validated analytical methods to ensure the verification of compliance with food law in official control activity. To this aim a previous gradient RP-HPLC method with DAD detection was modified and validated, according to international guidelines. The reliability of results was tested by analysing fish reference materials within the participation in European proficiency tests. The method has been used for the analysis of real samples consisting of several fishbased products with considerable differences in matrix composition. This characteristic is of great relevance to be able of apply the method in the field of official control.

18. Analysdagarna ( …, 2006

Altieri I, Castelli M, Semeraro A, Chiodo F, Menditto A, Patriarca M. Evaluation of uncertainty o... more Altieri I, Castelli M, Semeraro A, Chiodo F, Menditto A, Patriarca M. Evaluation of uncertainty of measurement from validation data: an application to clinical analyses. In: 18. Analysdagarna (Analytical Days). Abstracts ; June 12-15, 2006; Goteborg. 2006. p.142.

Food and Chemical Toxicology, 2013

Fish represents source of nutrients and major dietary vehicle of lipophilic persistent contaminan... more Fish represents source of nutrients and major dietary vehicle of lipophilic persistent contaminants. The study compared the effects of two legacy and two emerg ing fish pollutants (Hexabromocyclododecane HBCD; 2,2 0 ,4,4 0-Tetrabromodiphenyl ether BDE-47; 2,2 0 ,4,4 0 ,5,5 0-Hexachlorobiphe nyl PCB-153; 2,3,7,8-Tetrachlorodibenzo-p-doxin TCDD) in juvenile female mice exposed through a salmon based rodent diet for 28 days (dietary doses: HBCD 199 mg/kg bw/day; BDE-47 450 lg/kg bw/day; PCB-153 195 lg/kg bw/ day; TCDD 90 ng/kg bw/day). Dose levels were comparable to previously reported developmental Lowest Observed Adverse Effect Levels. None of the treatments elicited signs of overt toxicity, but HBCD increased relative liver weight. All compounds caused changes in liver, thy mus and thyroid; spleen was affected by BDE-47 and PCB-153; no effe cts were seen in uterus and adrenals. Strongest effects in thyroid follicles were elicited by PCB-153, in thymus and liver by BDE-47. HBCD and BDE-47 induced liver fatty changes, but appeared to be less potent in the other tissues. HBCD, BDE-47 and TCDD increased serum testosterone levels and the testosterone/estradiol ratio, suggesting a potential involvement of pathways related to sex steroid biosynthesis and/or metabolism. The results support the role of toxicological studies on juvenile rodents in the hazard characterization of chemicals, due to endocrine and/or immune effects.

Journal of Analytical Toxicology, 1997

A new analytical technique has been developed for the simultaneous determination of heroin, 6-mon... more A new analytical technique has been developed for the simultaneous determination of heroin, 6-monoacetylmorphine, morphine, morphine-6-and 3-g|ucuronides, and codeine in serum using liquid chromatography coupled with ionspray mass spectrometry. The analytes and the internal standard, nalorphine, were subjected to solid-phase extraction (SPE) using ethyl SPE columns before chromatography. The chromatographic separation of the analytes was achieved using a normal phase column and a water-methanol-acetonitrile-formic acid mobile phase at a flow rate of 230 IJL/min. The mass spectrometer was operated in selected-ion monitoring mode. Under these conditions, the limit of quantitation was 0.5 ng/mL for heroin, 4 ng/mt for 6-monoacetylmorphine, 4 ng/ml_ for morphine, 1 ng/mL for morphine-3-glucuronide, 4 ng/mL for morphine-6-glucuronide, and 4 ng/mL for codeine. Serum levels of heroin metabolites were determined in C57BL/6 inbred mice after a dose of 20 mg/kg heroin administered subcutaneously. 6-Monoacetylmorphine showed a peak concentration of 0.93 pg/mL serum at 3 rain, whereas morphine and morphine-3-glucuronide achieved their peak concentrations of 9.6 and 2.9 pg/mL serum at 10 and 20 rain, respectively. Finally, the absence of morphine-6-glucuronide and codeine excluded the possibility of their formation from morphine in this animal model.

Rapporti ISTISAN, 2005

Menditto A, Altieri I, Castelli M, Chiodo F, Patriarca M. Biomarker di esposizione e di dose effi... more Menditto A, Altieri I, Castelli M, Chiodo F, Patriarca M. Biomarker di esposizione e di dose efficace nelle prime fasi della vita. In: Maranghi F, Baldi F, Mantovani A, ed. Sicurezza alimentare e salute dell'infanzia. Roma: Istituto Superiore di Sanità; 2005. ( Rapporti ISTISAN 05/35). p.76-100.

Environmental Research, 1995

Journal of Analytical Toxicology, 1995

Indian Journal of Pharmaceutical Sciences, 2015

Arbutin, (4-hydroxyphenyl-β-D-glucopyranoside), the β-glucoside of hydroquinone (fig. 1), is pres... more Arbutin, (4-hydroxyphenyl-β-D-glucopyranoside), the β-glucoside of hydroquinone (fig. 1), is present in several plants [1] and has mainly an antiseptic action on urinary tract [2]. Arbutin is the main constituent of Arctostaphylos uva-ursi (L.) Spreng (Ericaceae), a medicinal plant known as bearberry, and in the market many phytotherapeutic products, made with its leaves, are present and are widely used to treat a variety of urinary disorders, such as urinary tract infections, inflammatory conditions of the efferent urinary tract, cystitis, urethritis, diuresis, lithuria, dysuria, acidic urine, pyelonephritis, kidney stones [3-5]. Arbutin is generally present in plants in combination with methylarbutin, especially in those belonging to the Ericaceae family [2]. It has been observed that in physiological conditions, after oral ingestion arbutin is absorbed by the gastrointestinal tract, it reaches the liver and then it is hydrolyzed to hydroquinone, which is finally conjugated to sulphuric and glucuronic acid by phase II enzymatic system. The 64-75% of orally ingested arbutin is excreted in urine as conjugated hydroquinone. In case of urinary tract infection, pathogenic bacteria, such as Escherichia coli, can break down the urea. As a consequence urine becomes alkaline and this pH change causes the release of the free form hydroquinone which possesses antibacterial activity [6-9] .

Therapeutic Drug Monitoring, 1998

A standardization of the analytical procedures for monitoring of fluoxetine and norfluoxetine ena... more A standardization of the analytical procedures for monitoring of fluoxetine and norfluoxetine enantiomers is described. Simultaneous determination of fluoxetine and norfluoxetine enantiomers in plasma and serum was performed by high-performance liquid chromatography with a chiral stationary phase, using ultraviolet absorbance detection. The analytes were extracted from the biologic matrix by alkalinization with NaOH and solid-phase extraction. Stability studies were conducted in EDTA, lithium-heparinized plasma and in serum spiked with the analytes stored at +4 degrees C for 1 week and at -20 degrees C for 1 month. Furthermore, stability studies in NaOH and in the extraction solvents were executed. Using this methodology, EDTA plasma is the most suitable matrix for drug monitoring, even if the storage should not exceed 3 weeks at -20 degrees C. Furthermore, the biologic sample should be left in NaOH for a short time before solid-phase extraction to prevent a degradation of matrix, which would interfere with the chromatographic analysis.

Annali dell'Istituto superiore di sanita, 2020

INTRODUCTION Turmeric is the common name for the rhizome of Curcuma longa L. In the recent years,... more INTRODUCTION Turmeric is the common name for the rhizome of Curcuma longa L. In the recent years, food supplements containing turmeric have been marketed and widely used by an increasing number of consumers. Spontaneous reports of suspected adverse reactions to food supplements are collected within the Phytovigilance system. METHODS An ad hoc multidisciplinary group investigated the suspected cases of hepatotoxicity reported to the Italian Phytovigilance system associated with the assumption of turmeric food supplements with the methodology specific to pharmacovigilance as well as for the evaluation of the quality and safety of food supplements. RESULTS A cluster of 28 spontaneous reports of acute hepatitis, mostly with cholestasis, associated with turmeric products were sent to the Italian Phytovigilance system in the first six months of 2019. In all cases, except one, the causality assessment was at least possible. The suspected products were collected and analysed for the presenc...

Journal of Chromatography B: Biomedical Sciences and Applications, 1991

Journal of Chromatography B: Biomedical Sciences and Applications, 1993

A rapid and selective assay of nicotine, cotinine and trans-3'-hydroxycotinine in human s... more A rapid and selective assay of nicotine, cotinine and trans-3'-hydroxycotinine in human serum, based on high-performance liquid chromatography with UV detection has been developed. The compounds were subjected to solid-phase extraction, using Extrelut 1 cartridges. Recoveries were ca. 95% for nicotine, 90% for cotinine and 50-55% for trans-3'-hydroxycotinine. The limit of quantitation observed with this method was 10 ng/ml for nicotine and 5 ng/ml for each of the metabolites. The compounds were also identified using high-performance liquid chromatography with particle beam mass spectrometry, to confirm their presence in human serum.

Journal of Liquid Chromatography & Related Technologies, 1996

ABSTRACT The simultaneous liquid chromatographic determination of the enantiomers of both fluoxet... more ABSTRACT The simultaneous liquid chromatographic determination of the enantiomers of both fluoxetine and its metabolite norfluoxetine in plasma samples of treated patients is described. The compounds are subjected to solid phase extraction before chromatography. The separation of the analytes is achieved using two chiralcel ODR columns on-line coupled and a mobile phase consisting of acetonitrile-NaClO4 0.3M (66/34 v/v, pH 2.5) at a flow rate of 0.6 mL/min. The compounds were detected by ultraviolet absorbance at 220 nm. The limit of quantification for each compound was 10ng/mL.

Forensic Science International, 1997