Ildikó Matušíková - Academia.edu (original) (raw)

Papers by Ildikó Matušíková

Long-term drought during one and two growing seasons, respectively strongly affected the timing a... more Long-term drought during one and two growing seasons, respectively strongly affected the timing and number of flushes of oak plants (Quercus robur L.), and negatively influenced subsequent growth. In contrast, strong compensation growth was observed upon rewatering. The defence response of experimental plants was assessed by analyzing stress metabolites and transcriptome changes. Our data revealed that while during mild and/or short drought stress only readily metabolizable carbohydrates e.g. glucose, fructose, and galactose are accumulated, prolonged drought stress results in accumulation of more stable and protective osmolytes such as quercitol and mannitol. At transcriptome level more than 3% of the expressed sequences tags (ESTs) represented on the microarray were differentially expressed reflecting the activation of defence responses in oak which appear to be stronger in the second year of persisting stress. The transcriptome changes are in good agreement with both the metabolic data as well as morphological observations. We conclude that mild long-term drought stress over two years triggers adaptation processes in oak plants that ensure survival, while irreversible damage is kept low facilitating full recovery upon rewatering.

Agriculture (Pol'nohospodárstvo), 2020

The cotton plant suffers from many pests and due to its economic importance, the use of efficient... more The cotton plant suffers from many pests and due to its economic importance, the use of efficient but ecologically friendly pesticides is highly desirable. The insecticide Sumi-Alpha with active compound Esfenvalerate is widely used for cotton treatment in Uzbekistan. This insecticide is considered as moderately hazardous for different organisms of ecosystems, while more collected data and relevant research is a necessity for judgement of their safety and/or potential risk. Therefore, a comparative analysis of cotton leaves was performed to study the protein profile changes upon treatment with this insecticide. Our data showed elevated accumulation of fructose bisphosphate aldolase and degradation of RuBisCo in the treated tissue. Besides, a protein belonging to P-loop containing nucleoside triphosphate hydrolases superfamily exerted lower accumulation. Our results indicate that the applied pyrethroid pesticide possibly can affect photosynthesis performance and cause accumulation of...

Evidence-Based Complementary and Alternative Medicine, 2020

Schisandra chinensis is a woody vine native to China, Korea, and Russia, which has been used as a... more Schisandra chinensis is a woody vine native to China, Korea, and Russia, which has been used as a traditional herbal remedy to treat male infertility. As very little information is available concerning its effects on ejaculated spermatozoa, the aim of this study was to investigate the chemical, antioxidant, and antibacterial properties of the S. chinensis berry (Omija) extract followed by an assessment of its in vitro effects on bovine sperm function and oxidative balance. Phytochemical components of the Omija extract were determined by high performance liquid chromatography. The content of polyphenols, flavonoids, and carotenoids was assessed by spectrophotometric protocols. Antioxidant characteristics of the Omija extract were determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and molybdenum-reducing antioxidant power (MRAP) assays. The disc diffusion method and determination of the minimal inhibitory concentration were applied to study the antibacterial properties of Schisand...

Cadmium belongs to important environmental pollutants, it can hinder plant fertilisation, growth ... more Cadmium belongs to important environmental pollutants, it can hinder plant fertilisation, growth of biomass and it has also influence on photosyntetic apparatus and metabolism of proteins and enzymes. The aim of our experiment was to evaluate differences in effects of two different concentrations of cadmium ions (50 and 100 mg.l. -1 ) on leaves of faba bean (Vicia faba L. cv. Aštar). In pot experiments we focused evaluated some common indicators of shoot growing (length, weight, size of leaf surface, number of stomatas and epidermal cells, stomatal index, size of stomatas, volume of photosyntetic pigments). Rates of stomatal index were higher on abaxialside of cd-treated leaves, in contrast were lower on adaxial side of leaves compared to control. We also observed statistically significant differences in length and width of stomata. In addition, the metal apparently negatively influenced the photosyntetic apparatus, since we observed reduction of pigment content, especially of chlor...

Israel Journal of Plant Sciences, 2021

Metal pollution represents a serious issue for sustainable agronomy and food safety. Activation o... more Metal pollution represents a serious issue for sustainable agronomy and food safety. Activation of plants’ protective mechanisms has been shown to depend on the extent of soil contamination with metal, but reports on dose-dependent responses (especially to extreme concentrations) are rather rare. In this research we exposed soybean (Glycine max L.) roots at a very early stage to a scale of cadmium concentrations, including doses far exceeding the amounts found in nature. Spectrophotometrical and enzyme in-gel detection assays were used to examine ongoing defence responses. The results confirmed commonly reported findings on inhibited growth and the activation of several superoxide dismutase isoforms in a dose-dependent manner. Contrary to expectations, the hydrogen peroxide levels, proline accumulation and the rate of lipid peroxidation were suppressed with increasing metal doses. More importantly, the linearity of Cd accumulation in soybean roots was interrupted at 200 mg.l−1 of ca...

Nova Biotechnologica et Chimica, 2017

Cadmium is a serious environmental pollutant and its uptake by plant represents a serious health ... more Cadmium is a serious environmental pollutant and its uptake by plant represents a serious health risk. Uptake, accumulation as well as sensitivity of soybean plants to metals have been shown to vary with genotype, while the dynamics of this uptake has rarely been studied. Here we studied the uptake and accumulation of Cd2+ ions in different parts of soybean plants of four cultivars Moravians, Gallec, Kent and Cardiff. The plants at early developmental stage were immersed in Hoagland nutrient solution in the presence or absence of 50 mg.L−1 and the isotope of 109Cd2+ to monitor its accumulation continuously at 24 h intervals for 10 days. Our results showed that the uptake rate varied among the cultivars, being the highest in roots of the cv. Moravians and the lowest in the cv. Gallec. We also observed a non-even distribution of radioactivity within the entire plants of individual cultivars. The most of Cd2+ isotope was translocated into primary leaves and leaves in the cvs. Kent and ...

Plant Science, 2021

This work presents the biochemical, cytochemical and molecular studies on two groups of PR protei... more This work presents the biochemical, cytochemical and molecular studies on two groups of PR proteins, β-1,3-glucanases and chitinases, and the arabinogalactan proteins (AGP) during the early stages of androgenesis induction in two breeding lines of rye (Secale cereale L.) with different androgenic potential. The process of androgenesis was initiated by tillers pre-treatments with low temperature, mannitol and/or reduced glutathione and resulted in microspores reprogramming and formation of androgenic structures what was associated with high activity of β-1,3-glucanases and chitinases. Some isoforms of β-1,3-glucanases, namely several acidic isoforms of about 26 kDa; appeared to be anther specific. Chitinases were well represented but were less variable. RT-qPCR revealed that the cold-responsive chitinase genes Chit1 and Chit2 were expressed at a lower level in the microspores and whole anthers while the cold-responsive Glu2 and Glu3 were not active. The stress pre-treatments modifications promoted the AGP accumulation. An apparent dominance of some AGP epitopes (LM2, JIM4 and JIM14) was detected in the androgenesis-responsive rye line. An abundant JIM13 epitopes in the vesicles and inner cell walls of the microspores and in the cell walls of the anther cell layers appeared to be the most specific for embryogenesis.

Polish Journal of Environmental Studies, 2018

Arsenic is a serious soil pollutant with toxic effects on biological systems. Elevated soil conce... more Arsenic is a serious soil pollutant with toxic effects on biological systems. Elevated soil concentrations may negatively affect crop production and food safety. The impact of arsenic on plants depends on many factors, including nitrogen availability. Nitrogen (N) as an essential mineral affects overall energetics of plants, while its non-optimal doses have been shown to also impact plant performance and yield, as well as tolerance to environmental constraints. The combined effects of these two factors, however, have been rarely studied. Here we investigated the impact of sublethal doses of As 3+ (5 mM) on wheat plants grown in hydropony, applying a set of 8 different N concentrations spanning from starvation (0 mM N in the media) through optimum (7.5 mM N) to excessive amounts (up to 35 mM N). The results showed that the content of photosynthetic pigments varies depending on N concentration and As 3+ presence. The different energetic status of plants also affected the final As uptake. Establishing nutrition conditions might be important for limiting metal(loid) uptake from soil in contaminated areas.

Plant Cell, Tissue and Organ Culture (PCTOC), 2018

Maize grain is of a great economic importance worldwide. Morphometric and cytological description... more Maize grain is of a great economic importance worldwide. Morphometric and cytological descriptions of developing maize endosperms are available in inbred lines growing under natural conditions. Despite tremendous potential in biotechnology, similar studies focused on endosperm development in vitro are rather rare. Here we provide a comparative study on growth and development of endosperms in planta versus in vitro, considering regulation by different cytokinins during endosperm cultivation. Histochemical analyses were performed on hand-cut sections of samples stained with Sudan III. Microtubules (MTs) were immunolocalized in whole mount samples and Steedmanʼs wax sections of endosperm and miniendosperm from in vitro conditions. Our results confirmed that in vitro cultured maize endosperms proliferate and establish aleurone and starchy cells like endosperms grown in planta, however later ones undergo a distinct developmental program triggered/ controlled by cytokinins. Higher mitotic activity of surface layer cells resulted in the miniendosperm proliferation, while the centrally localized parenchymatic starchy-like cells differentiated into tracheary elements (TEs). Furthermore, we report, for the first time, on the formation of numerous lobes and trichomes on pericarp cells. MT rearrangements play an important regulatory role in the differentiation of TEs. Formation of functional vascular tissue and lipid accumulation appears to be fundamental for maize miniendosperm proliferation. In contrast, the presence of maternal tissue (pericarp and nucellus) prevents miniendosperm growth and development. We assume the formation of lobes and trichomes is an adaptive response of in vitro maize endosperm to high temperatures.

Agriculture (Pol'nohospodárstvo), 2017

Defense components such as chitinases (EC 3.2.1.14) are crucial for plants to cope diseases. Desp... more Defense components such as chitinases (EC 3.2.1.14) are crucial for plants to cope diseases. Despite of that the pattern and activities of these enzymes in agronomically important Triticale is unexplored. This work is aimed to study chitinase activities in the leaves of plants of early developmental stages in two diploids (Aegilops tauschii Coss., Triticum monococcum L.), four tetraploids (Ae. cylindrical Host, Ae. triuncialis L., T. araraticum Jakubyz, T. dicoccum Schrank) and two hexaploids (T. aestivum L., T. spelta L.). The leaves were subjected to quantitative and qualitative activity assays using synthetic 4-methylumbelliferyl-β-D-N,N´,N´´-triacetylchitotrioside and glycolchitin as substrates, respectively. Our results showed that the activities of chitinases with specificity towards short oligomers were variable and genotype dependent. The enzyme activities in the tetra- and hexaploid genotypes were significantly higher than in diplod counterparts. In the gel detection assays...

Journal of Microbiology, Biotechnology and Food Sciences, 2017

Chitinases are enzymes widely spread in plants with no endogenous substrate and play significant ... more Chitinases are enzymes widely spread in plants with no endogenous substrate and play significant role to lyse the cell walls of many pathogens. Their role in defense is firmly established. Recently, their functions in plants have been extended to response to abiotic stresses and various developmental plant processes. The gene family of chitinases is well characterized in some model species. Few researches on their involvement in cotton defense against pathogens have been reported. Here were investigated Gossypium raimondii genome in the Phytozome database for the presence of homologues. BLAST similarity search, using five chitinase representatives from Arabidopsis belonging to different classes I-V, identified a total of 41 non-redundant chitinase gene sequences in cotton. We analysed them in silico using available bioinformatics software and characterized their basic molecular structures. Signal peptides, carbohydrate-binding domains, hinge regions were predicted, molecular weights...

Nova Biotechnologica et Chimica, 2016

The (1,3)-β-D-glucan also referred to as callose is a main component of cell walls of higher plan... more The (1,3)-β-D-glucan also referred to as callose is a main component of cell walls of higher plants. Many physiological processes are associated with the changes in callose deposition. Callose is synthesised by the callose synthase complex while its degradation is regulated by the hydrolytic enzymes β-1,3-glucanases. The latter one specifically degrade (1,3)-β-D-glucans. This work is aimed to study β-1,3-glucanase activities in the leaves of plants at two leaf stage in two diploids (

Planta, 2016

Chitinase gene from the carnivorous plant, Drosera rotundifolia , was cloned and functionally cha... more Chitinase gene from the carnivorous plant, Drosera rotundifolia , was cloned and functionally characterised. Plant chitinases are believed to play an important role in the developmental and physiological processes and in responses to biotic and abiotic stress. In addition, there is growing evidence that carnivorous plants can use them to digest insect prey. In this study, a full-length genomic clone consisting of the 1665-bp chitinase gene (gDrChit) and adjacent promoter region of the 698 bp in length were isolated from Drosera rotundifolia L. using degenerate PCR and a genome-walking approach. The corresponding coding sequence of chitinase gene (DrChit) was obtained following RNA isolation from the leaves of aseptically grown in vitro plants, cDNA synthesis with a gene-specific primer and PCR amplification. The open reading frame of cDNA clone consisted of 978 nucleotides and encoded 325 amino acid residues. Sequence analysis indicated that DrChit belongs to the class I group of plant chitinases. Phylogenetic analysis within the Caryophyllales class I chitinases demonstrated a significant evolutionary relatedness of DrChit with clade Ib, which contains the extracellular orthologues that play a role in carnivory. Comparative expression analysis revealed that the DrChit is expressed predominantly in tentacles and is up-regulated by treatment with inducers that mimick insect prey. Enzymatic activity of rDrChit protein expressed in Escherichia coli was confirmed and purified protein exhibited a long oligomer-specific endochitinase activity on glycol-chitin and FITC-chitin. The isolation and expression profile of a chitinase gene from D. rotundifolia has not been reported so far. The obtained results support the role of specific chitinases in digestive processes in carnivorous plant species.

Plant Growth Regulation, 2016

75 and 5.25 mM N), optimal N (7.5 mM N) as well as excess (15, 30 and 35 mM N) N supply in the fo... more 75 and 5.25 mM N), optimal N (7.5 mM N) as well as excess (15, 30 and 35 mM N) N supply in the form of nitrate. Our results show that several isoforms of both enzymes in wheat leaves and/or shoots clearly depended on N supply, while their activities rather depended on organ type. Furthermore, glucanases and chitinases appeared to be regulated in an opposite way. The activities of particular chitinases and glucanases correlated with a proline content that has multiple functions in plants. Proline typically accumulated with increasing the N supply when certain excessive N doses induced the gene for proline synthase (P5CS) in shoots and that for ornithine aminotransferase (OAT) in roots. This work points to a N-dependent activity of several defense-related compounds suggesting the possibly of altered plant defense potential under various N regimes.

Journal of microbiology, biotechnology and food sciences, 2016

Application of insecticides used in cotton fields is often associated with secondary biotic stres... more Application of insecticides used in cotton fields is often associated with secondary biotic stresses. One of possible reasons of such phenomenon is explained by decreased contents of plants’ defense components. As peroxidase (POD) and polyphenoloxidase (PPO) are typical oxidoreductase enzymes scavenging cell oxidative damage, we studied their change levels in cotton leaves in response to the application of three insecticides field experiment. Moreover, the concentration of proline (Pro), methionine (Met) and cysteine (Cys) was studied. The plants were treated with Carbophos, Lannate and Sumi-alfa in early blooming stage at commonly used doses in. Leaf samples were taken on the 10thand 13th days of the treatment. A pyrethroid insecticide Sumi-alfa appeared to negatively impact activities of both POD and PPO (P≤0.05), contrasting the other two insecticides examined. On the other hand, levels of amino acids with antioxidative properties increased after application of all three insectic...

Journal of Agricultural and Food Chemistry, 2015

The impact of severe drought stress (13% soil moisture) on the physiological responses, metabolic... more The impact of severe drought stress (13% soil moisture) on the physiological responses, metabolic profile, and pathogenesis-related (PR) proteins in wheat above- and below-ground biomass after 20 days of treatment was studied. Drought depleted growth, assimilation pigments, and majority of free amino acids in the shoots (but proline increased considerably, +160%). On the contrary, root growth parameters were elevated, and free amino acids did not decrease, indicating investment of metabolites into the growth of roots under water deficiency. Mineral nutrients were only slightly influenced. Profiling of pathogenesis-related (PR) proteins revealed that chitinases (EC 3.2.1.14) and glucanases (EC 3.2.1.39) were activated in wheat by drought. Individual isoforms and their activity were rather stimulated under drought, especially in shoots. The expression of selected genes is in agreement with enzymatic data and suggests an organ (tissue) specific- and opposing behavior of these two types of defense components in drought-stressed wheat. Metabolic analyses at the level of phenolics showed an increase in the free and bound fraction of phenolic acids almost exclusively in the shoots and flavonoid isoorientin increased considerably: protective action against oxidative stress and dehydration of the leaves seems to be the main reason for this finding. The role of PR proteins and phenolics in drought-stressed tissue is discussed.

Progress in Environmental Science, Technology and Management, 2012

Agricultural Research, 2015

Chitin is the main component of the fungi cell wall and exoskeleton of insects which serve as a d... more Chitin is the main component of the fungi cell wall and exoskeleton of insects which serve as a defensive covering means in these organisms. Plants produce chitinase and glucanase as a systemic acquired resistance against these pests. The amounts of these enzymes in plant tissues change by the influences of different biotic and abiotic environmental factors which result in the defense of plants to be higher or lower. Insecticides a class of pesticides used against insects often cause the insect populations to increase. The reason of the phenomenon is explained with the fact that plants become more nutritious and defensive compounds lower. We investigated the influence of three insecticides carbophos (organophosphate), lannate (carbamate) and sumi-alfa (pyrethroid) on the hydrolase enzymes: chitinase and-1,3-glucanase, which determine the defense mechanism of cotton plant to fungi and insects. Field experiments were conducted on the cotton plant at the prebloom stage. Colorimetric analysis showed that the quantity of soluble proteins in cotton leaves treated with the pyrethroid sumi-alfa increased, whereas carbophos and lannate treatment decreased. The enzyme activities of chitinase increased after treatment with carbophos and lannate as compared to control, whereas sumi-alfa caused to decrease.-1,3-glucanase activity of the leaves treated with lannate and sumi-alfa was lower than the control.

Transgenic potato plants (Solanum tuberosum L.) of selected Slovak cultivars (cvs.) and one breed... more Transgenic potato plants (Solanum tuberosum L.) of selected Slovak cultivars (cvs.) and one breeding line were obtained by Agrobacterium-mediated transformation. In vitro-grown plants of cvs. Albina, Eta, Malvina, Vila and line 116/86 were tested for their ability to regenerate transgenic plants. Infection of leaf explants and internodes except of those of cv. Albina resulted in the regeneration of transgenic shoots. Transformation efficiency was genotype dependent. A greater number of transformants was obtained from line 116/86 using leaf disc transformation than was obtained from the reference cultivar Desirere. The activity of GUS transgene was measured in populations of transgenic plants 116/86(GHN) and Desire¿e(GHN). The genotype had no significant influence on GUS transgene expression levels. High transgene copy number resulted in increased variation of transgene expression among plants 116/86(GHN).

Journal of microbiology, biotechnology and food sciences

In this work we used the Cre/loxP recombination system to study the activity of the Arabidopsis D... more In this work we used the Cre/loxP recombination system to study the activity of the Arabidopsis DLL promoter under water stress treatment. For this, the T-DNA containing the Cre/loxP self-excision recombination cassette was introduced into tobacco genome via A. tumefaciens LBA 4404. The expression of the cre gene was regulated by the DLL promoter. On activity of the DLL the Cre recombinase was expected to remove Cre/loxP cassette. Transgenic nature of regenerated transgenic T0 tobacco plantlets was proved by GUS and PCR analyses. The selected 10 transgenic T0 plants were subjected to the water stress analyses under in vitro as well as under in vivo conditions. The osmotic stress experiments were performed with 10 % PEG and 100 mmol.l-1 mannitol (individually). The activity of the DLL was evaluated after 24 hours. For drought stress experiments, the watering was withheld for 10 days. The activity of the DLL was monitored using PCR approach. Under given abiotic stress conditions, no a...