Iman Behiry - Academia.edu (original) (raw)

Papers by Iman Behiry

Laboratory Medicine, 2019

BackgroundInvasive fungal infections (IFIs) are a main cause of morbidity and mortality. High-res... more BackgroundInvasive fungal infections (IFIs) are a main cause of morbidity and mortality. High-resolution melting polymerase chain reaction (HRM PCR) is promising for the identification of fungal species via the detection of internal transcribed spacer 2 (ITS2).ObjectivesTo assess the sensitivity and specificity of HRM PCR in diagnosing IFIs, compared with blood culture.MethodsOur study included 100 patients who were suspected of having IFIs; we analyzed their specimens via blood culture and HRM PCR.ResultsBlood culture results were positive in 57 cases and negative in 43 cases. HRM PCR results were positive in 14 cases and negative in 86 cases. The 14 cases with positive results included 4 with Candida tropicalis, 4 with Candida glabrata, and 6 with Candida krusei. HRM PCR sensitivity was 24.6%, specificity was 100%, positive predictive value (PPV) was 100%, and negative predictive value (NPV) was 50%.ConclusionsHRM PCR is specific but not sensitive. Blood culture is more sensitive ...

Journal of Analytical Science and Technology, Mar 18, 2017

Background: Carbapenem-resistant Enterobacteriaceae have been increasingly reported worldwide sin... more Background: Carbapenem-resistant Enterobacteriaceae have been increasingly reported worldwide since their first identification more than 20 years ago. The early identification of carriers and implementing of cohorting strategies is the only means to prevent nosocomial outbreaks caused by carbapenemase.The aim of this work is to evaluate the prevalence of intestinal colonization with carbapenemase-producing Enterobacteriaceae (CPE) in non-hospitalized patients and assesses a microbiological protocol for screening these isolates in fecal material. Methods: This study was conducted on patients seen at Kasr Alaini hospital and investigated at microbiology laboratory in Cairo University Hospitals, from May 2013 to October 2013. The study was conducted on 600 patients; 450 of them were non hospitalized patients (group I) and came for regular checkup, and 150 were inpatients as control group (group II) from different hospital departments. Results: Based on questionnaire feedback, history and clinical findings, certain patients were suspected to have CPE. Group I includes 337 (56.2%) males and 113 (18.8%) females and group II includes 83 (13.8%) males and 67 (11.2%) females. Age of group I ranged from 18 to 82 years with a mean of 44 years, while the age of group II ranged from 10 to 77 years with a mean of 37 years. Out of 600 fecal samples (450 from group I and 150 from group II) 12 (carriage rate 2%) were positive by disk diffusion methods, Chrom agar-KPC and or Modified Hodge Test (MHT) and PCR. Two of them were non hospitalized patients (group I) (carriage rate 0.33%) and 10 were hospitalized patients (control group) (carriage rate 1. 7%) from different hospital departments. Conclusions: We describe a fecal carriage of carbapenam producing Enterobactericae ,all of them are NAD producers orOXA-48 producers in patients non infected by these organisms.

Evidence Based Womenʼs Health Journal, 2012

Background The administration of antibiotic prophylaxis to laboring women who harbor group B stre... more Background The administration of antibiotic prophylaxis to laboring women who harbor group B streptococci (GBS) depends on the identification of carriers. Objectives We sought to evaluate the diagnostic accuracy of the culture methods and the antigen agglutination test for the detection of GBS in pregnant women using a PCR assay. Patients and methods A prospective cross-sectional study of women at 35-37 weeks of gestation and fulfilling the selection criteria. Two vaginal swabs were collected from each woman: one for microbiological examination and the other for direct blood agar culture and broth inoculation. The inoculated broth was used for subculture, antigen detection by the latex agglutination test, and an scpB gene PCR assay. Isolated GBS were tested for their antimicrobial susceptibility test. Results A total of 30 out of 96 pregnant women were subjected to this study according to their inclusion criteria. The highest positive percent of GBS colonization was 36.6% detected by the scpB gene PCR assay, which was considered our standard, 33.3% by the antigen detection test, 26.7% by the culture method from the inoculated broth, and 23.3% from the direct culture method. The antigen detection from the inoculated broth by the latex agglutination test was the most accurate test compared with the other test methods, with a sensitivity of 90.9% (95% confidence interval 80.4-100) and 100% (95% confidence interval 96.4-100) for both the specificity and the positive predictive value, and a diagnostic accuracy of 96.7%. All detected GBS were 100% sensitive to penicillin and vancomycin, with 54.5% resistant to clindamycin and 45.5% resistant to erythromycin. However, there was no significant association between the clinical data and the GBS colonization. Conclusion Routine screening of pregnant women for vaginal GBS colonization by the antigen detection method from the inoculated broth, and treatment of positive cases with the proper antimicrobial drugs to prevent a possible neonatal infection, which may be acquired during delivery, should be carried out.

Antimicrobial Agents and Chemotherapy, 1992

Jundishapur Journal of Microbiology, 2014

Background: AmpC type β-lactamases are commonly isolated from extended-spectrum Cephalosporin-res... more Background: AmpC type β-lactamases are commonly isolated from extended-spectrum Cephalosporin-resistant Gram-negative bacteria. Also, resistance appeared in bacterial species not naturally producing AmpC enzymes. Therefore, a standard test for the detection of the plasmid-mediated AmpC enzyme and new breakpoints for extended spectrum Cephalosporins are urgently necessary. Objectives: To detect plasmid and chromosomal mediated AmpC-β-lactamases in Gram negative bacteria in community and hospital acquired infections. Materials and Methods: 1073 Gram negative clinical isolates were identified by the conventional methods and were screened for AmpC production using Cefoxitin discs. Confirmatory phenotypic identifications were done for the Cefoxitin-resistant isolates using Boronic Acid for combined and double disc synergy tests, Cloxacillin based double disc synergy test, and induction tests. The genotypic identification of plasmid-mediated AmpC was done using multiplex PCR. ESBL production was also screened by discs of Ceftazidime and Cefotaxime with and without Clavulanic Acid (10 μg). Results: The AmpC-producing isolates among all identified Gram negative bacilli were 5.8% (62/1073) as detected by screening disc diffusion methods, where 72% were positive for AmpC by combined disc method (Cefotetan and Boronic Acid), 56.5% were positive by each of Boronic Acid and Cloxacillin double disc synergy tests, 35.5% were positive by the induction test, and 25.8% were plasmid-mediated AmpC β-lactamase producers by the multiplex PCR. Plasmid-mediated AmpC genes retrieved, belonged to the families (MOX, FOX, EBC and CIT). ESBL producers were found in 26 (41.9%) isolates, 15 (57%) of which also produced AmpC. Isolates caused hospital acquired infections were (53/62); of which (39/62) were AmpC producers. While only (8/62) of the isolates caused community-acquired infections, were AmpC producers, and (1.6%) (1/62) were non AmpC producer. Conclusions: The AmpC β-lactamases detection tests had to be included in the routine microbiology workup of Gram negative bacteria, namely Cefoxitin as a screening test, combined Boronic Acid disc test with Cefotetan, followed by synergy tests and finally by the induction test for phenotypic identifications. Multiplex PCR can successfully detect the plasmid AmpC genes.

The Scientific World JOURNAL, 2011

In this study we isolate and identify the EnteropathogenicEscherichia coli(EPEC) causing diarrhea... more In this study we isolate and identify the EnteropathogenicEscherichia coli(EPEC) causing diarrhea in children less than five years in Cairo, Egypt, during different seasons. Children younger than five years with diarrhea, attending the Pediatric Gastroenterology Intensive Care Unit of the Cairo University Pediatric Hospital in one year period were our group of study. Our control group was age and sex matched concurrent healthy children. The identifiedE. coliisolates were subjected to antimicrobial disc diffusion susceptibility test and further identified for EPEC serotype by slide agglutination test, using antiserumE. colisomatic trivalent I (O111, O55, O26) according to the instructions of the manufacturer. Out of 134 patients 5.2% of them revealed EPEC in the fecal sample, while the 20 children control group showed no EPEC isolates in their samples. Our EPEC frequency showed variations from the compared results of other studies. Higher rate of EPEC (18.7%) was found in patients be...

Open Access Macedonian Journal of Medical Sciences, 2021

BACKGROUND: Serum 1, 3-β-D-glucan (BDG) assay was recommended for diagnosing fungal infections. A... more BACKGROUND: Serum 1, 3-β-D-glucan (BDG) assay was recommended for diagnosing fungal infections. AIM: We aimed to assess 1, 3-β-D-glucan in bronchoalveolar lavage (BAL) fluid in invasive pulmonary aspergillosis (IPA). METHODS: Out of 104 patients clinically suspected fungal, 45 were probable, 18 possible, and 41 unlikely according to EORTC/MSG 2008 criteria. Measuring BAL BDG and galactomannan were done. RESULTS: The sensitivity, specificity, and positive and negative predictive values (PPV and NPV) for BDG were 44%, 71%, 62%, and 54%; for galactomannan 84%, 83%, 84%, and 83%; and 93%, 66%, 75%, and 90%, respectively, when combining both tests. A significant different performance of GM; p = 0.0008 was detected in patients with malignant disorders when compared to non-malignant; but not for BDG; p = 0.121. CONCLUSION: We can conclude that BAL-BDG is helpful if positive in a clinically suspected IFI case, but if negative cannot rule out fungal infection. Thus, combining results of BAL-...

Rinsho byori. The Japanese journal of clinical pathology

ABSTRACT

T HE MAIN concept of this study was to assess the potentiality of hydrolytic enzymes as virulence... more T HE MAIN concept of this study was to assess the potentiality of hydrolytic enzymes as virulence factors for Aspergillus species during aspergillosis infection process. Forty Aspergillus species were isolated from medical (15 isolates) as well as environmental isolates (25 isolates) from soils, outdoor and indoor environments. Extracellular proteases, phospholipases and esterase activities were measured. The pathogenicity of some Aspergillus species was in vivo assessed. It was represented in mean survival times, mortality percentages, fungal counts in different organs, and histopathological examination of lung tissue. The expression levels of protease, phospholipase and esterase genes were studied by real time PCR. Four physiologically significant isolates were selected out of forty and were identified up to molecular level. Aspergillus ochraceus fm90 recorded the highest pathogenicity as represented by mortality percentages and mean survival times of mice, while A. flavus fm90 was the least pathogenic one. Expression of genes of protease, phospholipase and esterase was found to be greater in A. ochraceus fm90 than in A. flavus fm90. It can be concluded that pathogenicity is probably related to physiological (enzymatic) activities of the isolates. Also, variation in expression levels of protease and phospholipase genes in A. ochraceus fm90 and A. flavus fm90 could denote their possible involvement in the pathogenicity process.