Inain Jaies - Academia.edu (original) (raw)

Papers by Inain Jaies

Research paper thumbnail of Diagnosis of Aeromoniasis in Common Carp Fish by Indirect ELISA Test through Antibody Detection

Uttar Pradesh journal of zoology, Jun 20, 2024

Aeromonas hydrophila, a significant pathogen in aquaculture, poses severe threat to cultured fish... more Aeromonas hydrophila, a significant pathogen in aquaculture, poses severe threat to cultured fish species due to its high mortality rates and broad temperature tolerance. Traditional detection methods for A. hydrophila are time-consuming, require specific expertise, and are difficult to interpret. In this study, we established an indirect enzyme-linked immunosorbent assay (ELISA) method for the rapid and sensitive detection of A. hydrophila using polyclonal antibodies. Fish samples exhibiting clinical symptoms of Aeromoniasis were collected and subjected to microbiological and molecular analysis for pathogen identification. Polyclonal antibodies against A. hydrophila were procured and optimized for ELISA. The reaction conditions were meticulously explored through a square matrix titration test to determine the optimal dilutions of antigen and antibody. Results indicated that the ELISA exhibited specificity towards A. hydrophila, with a defined cutoff value of 0.2481 for positive samples. The developed assay offers a reliable tool for the early detection of A. hydrophila infections in aquaculture, facilitating timely management strategies and mitigating economic losses.

Research paper thumbnail of Freshwater Faunal Diversities as Pollution Indicators: A Special Reference to Microfauna

CRC Press eBooks, Apr 30, 2024

Research paper thumbnail of On Relationship between Quality of Sleep and Physical Activities among the Students of Kashmir Valley

Journal of Nursing Research Patient Safety and Practise, Oct 12, 2023

The present study explores the intricate relationship between the quality of sleep and physical a... more The present study explores the intricate relationship between the quality of sleep and physical activity. Both sleep and physical activity are integral components of a healthy lifestyle, and their interaction has been a subject of growing interest in research. The aim of this paper was to delve into the impact of sleep on physical activity and vice versa, highlighting the importance of maintaining a balance between these two aspects for overall well-being. The study revealed that there was a significant negative correlation between physical activity and sleep quality (r = −0.184, P<0.01) i.e., we can say physical activity can positively predict psychological resilience. This paper also discusses practical strategies to optimize both sleep quality and physical activity levels.

Research paper thumbnail of Immune Components and Defense Mechanism in Fish: An Overview

Apple Academic Press eBooks, Sep 12, 2023

Research paper thumbnail of Infectious Diseases of Coldwater Fishes: Focus on Viral and Fungal Infections

Coldwater Fisheries and Aquaculture Management

Research paper thumbnail of Immunological and molecular diagnostic techniques in fish health: present and future prospectus

Molecular biology reports, Apr 20, 2024

Research paper thumbnail of Phytoremediation potential of macrophytes against heavy metals, nitrates and phosphates: A review

Environment Conservation Journal, Jan 8, 2023

Research paper thumbnail of Isolation of Aeromonas hydrophila infecting the fresh-water fishes in the Jammu region of Indian Himalayas

SKUAST JOURNAL OF RESEARCH

Research paper thumbnail of Pathological alterations induced by Saprolegnia parasitica in the skin and gills of rainbow trout of Kashmir Himalayas, India: A scanning electron microscopic study

Research paper thumbnail of Immuno-modulation in fishes against parasitic infections: A review

Parasites are the opportunist organisms that attack on varied number of fish species and lead to ... more Parasites are the opportunist organisms that attack on varied number of fish species and lead to various physiological and anatomical changes in them. It is well documented that fish parasites are associated with the alterations in haematological, biochemical and histopathological changes in fish communities all over the world. By acing as secondary pathogens, they have been linked with the imunomodulation in fishes. This review presents a thorough literature citing the various instances of immunomodulations in fishes against parasites.

Research paper thumbnail of Bactericidal Potential of Local Medicinal Plants of Kashmir Valley in Fishes: A Review

Current Journal of Applied Science and Technology

Bacterial diseases in fishes create one of the alarming situations that render significant loss t... more Bacterial diseases in fishes create one of the alarming situations that render significant loss to the fishing industries and aquacultural farm units worldwide. Various drugs have been formulated, standardized, and used in the industry to combat bacterial infections. However, non-judicious drugs can lead to pharmacological problems and induction of toxicity in fishes, leaving them less suitable for human consumption. These drugs can be supplemented with the natural herb plants having antibacterial activities, although they cannot be used solely to curb the menace. Therefore, the review put forth an idea, which plant herbs can be used as antibacterial drugs in fishes.

Research paper thumbnail of PCR Based Identification of Saprolegnia parasitica Affecting Rainbow Trout Aquaculture in Kashmir Region of Indian Himalayas

Indian Journal of Animal Research

Saprolegnia parasitica infecting Rainbow trout (Oncorhynchus mykiss), has been a major bottleneck... more Saprolegnia parasitica infecting Rainbow trout (Oncorhynchus mykiss), has been a major bottleneck in the intensification and propagation of trout culture in northern Himalayas of India. Present study was to ascertain the presence of S. parasitica infections using molecular diagnostic techniques in cultured rainbow trouts collected from different hatcheries of Kashmir valley. Growth of S. parasitica at different incubation temperatures was examined. The molecular detection of fungus done by using PCR, revealed that the twenty trout specimens out of 144 collected samples were found infected with Saprolegnia parasitica with overall prevalence of 13.88%, with a PCR product size of around 750 bp. No growth of S. parasitica was reported at 4°C after 48 h of incubation. S. parasitica infections were more frequent in cold water months when water temperature was recorded between 7°C – 9°C.

Research paper thumbnail of Diagnosis of Aeromoniasis in Common Carp Fish by Indirect ELISA Test through Antibody Detection

Uttar Pradesh journal of zoology, Jun 20, 2024

Aeromonas hydrophila, a significant pathogen in aquaculture, poses severe threat to cultured fish... more Aeromonas hydrophila, a significant pathogen in aquaculture, poses severe threat to cultured fish species due to its high mortality rates and broad temperature tolerance. Traditional detection methods for A. hydrophila are time-consuming, require specific expertise, and are difficult to interpret. In this study, we established an indirect enzyme-linked immunosorbent assay (ELISA) method for the rapid and sensitive detection of A. hydrophila using polyclonal antibodies. Fish samples exhibiting clinical symptoms of Aeromoniasis were collected and subjected to microbiological and molecular analysis for pathogen identification. Polyclonal antibodies against A. hydrophila were procured and optimized for ELISA. The reaction conditions were meticulously explored through a square matrix titration test to determine the optimal dilutions of antigen and antibody. Results indicated that the ELISA exhibited specificity towards A. hydrophila, with a defined cutoff value of 0.2481 for positive samples. The developed assay offers a reliable tool for the early detection of A. hydrophila infections in aquaculture, facilitating timely management strategies and mitigating economic losses.

Research paper thumbnail of Freshwater Faunal Diversities as Pollution Indicators: A Special Reference to Microfauna

CRC Press eBooks, Apr 30, 2024

Research paper thumbnail of On Relationship between Quality of Sleep and Physical Activities among the Students of Kashmir Valley

Journal of Nursing Research Patient Safety and Practise, Oct 12, 2023

The present study explores the intricate relationship between the quality of sleep and physical a... more The present study explores the intricate relationship between the quality of sleep and physical activity. Both sleep and physical activity are integral components of a healthy lifestyle, and their interaction has been a subject of growing interest in research. The aim of this paper was to delve into the impact of sleep on physical activity and vice versa, highlighting the importance of maintaining a balance between these two aspects for overall well-being. The study revealed that there was a significant negative correlation between physical activity and sleep quality (r = −0.184, P<0.01) i.e., we can say physical activity can positively predict psychological resilience. This paper also discusses practical strategies to optimize both sleep quality and physical activity levels.

Research paper thumbnail of Immune Components and Defense Mechanism in Fish: An Overview

Apple Academic Press eBooks, Sep 12, 2023

Research paper thumbnail of Infectious Diseases of Coldwater Fishes: Focus on Viral and Fungal Infections

Coldwater Fisheries and Aquaculture Management

Research paper thumbnail of Immunological and molecular diagnostic techniques in fish health: present and future prospectus

Molecular biology reports, Apr 20, 2024

Research paper thumbnail of Phytoremediation potential of macrophytes against heavy metals, nitrates and phosphates: A review

Environment Conservation Journal, Jan 8, 2023

Research paper thumbnail of Isolation of Aeromonas hydrophila infecting the fresh-water fishes in the Jammu region of Indian Himalayas

SKUAST JOURNAL OF RESEARCH

Research paper thumbnail of Pathological alterations induced by Saprolegnia parasitica in the skin and gills of rainbow trout of Kashmir Himalayas, India: A scanning electron microscopic study

Research paper thumbnail of Immuno-modulation in fishes against parasitic infections: A review

Parasites are the opportunist organisms that attack on varied number of fish species and lead to ... more Parasites are the opportunist organisms that attack on varied number of fish species and lead to various physiological and anatomical changes in them. It is well documented that fish parasites are associated with the alterations in haematological, biochemical and histopathological changes in fish communities all over the world. By acing as secondary pathogens, they have been linked with the imunomodulation in fishes. This review presents a thorough literature citing the various instances of immunomodulations in fishes against parasites.

Research paper thumbnail of Bactericidal Potential of Local Medicinal Plants of Kashmir Valley in Fishes: A Review

Current Journal of Applied Science and Technology

Bacterial diseases in fishes create one of the alarming situations that render significant loss t... more Bacterial diseases in fishes create one of the alarming situations that render significant loss to the fishing industries and aquacultural farm units worldwide. Various drugs have been formulated, standardized, and used in the industry to combat bacterial infections. However, non-judicious drugs can lead to pharmacological problems and induction of toxicity in fishes, leaving them less suitable for human consumption. These drugs can be supplemented with the natural herb plants having antibacterial activities, although they cannot be used solely to curb the menace. Therefore, the review put forth an idea, which plant herbs can be used as antibacterial drugs in fishes.

Research paper thumbnail of PCR Based Identification of Saprolegnia parasitica Affecting Rainbow Trout Aquaculture in Kashmir Region of Indian Himalayas

Indian Journal of Animal Research

Saprolegnia parasitica infecting Rainbow trout (Oncorhynchus mykiss), has been a major bottleneck... more Saprolegnia parasitica infecting Rainbow trout (Oncorhynchus mykiss), has been a major bottleneck in the intensification and propagation of trout culture in northern Himalayas of India. Present study was to ascertain the presence of S. parasitica infections using molecular diagnostic techniques in cultured rainbow trouts collected from different hatcheries of Kashmir valley. Growth of S. parasitica at different incubation temperatures was examined. The molecular detection of fungus done by using PCR, revealed that the twenty trout specimens out of 144 collected samples were found infected with Saprolegnia parasitica with overall prevalence of 13.88%, with a PCR product size of around 750 bp. No growth of S. parasitica was reported at 4°C after 48 h of incubation. S. parasitica infections were more frequent in cold water months when water temperature was recorded between 7°C – 9°C.