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Carotenoids are commercially used as natural colourant, antioxidant and as a vitamin A precursors... more Carotenoids are commercially used as natural colourant, antioxidant and as a vitamin A precursors. In the present investigation, critical factors for high total carotenoid yield from the effect of stress created by high pH, excess NaNO3 and K2HPO4 were investigated by using single factor experiment and later optimization were carried out by using Box-Behnken experimental design from Leptolyngbya sp. BTA 287 belonging to Indo-Burma Biodiversity hot spot. This strain was found to produce maximum total carotenoids at pH 9, NaNO3 concentration of 7.50 mg/ml and K2HPO4 concentration at 0.20 mg ml -1 in BG-11 medium, yielding highest total carotenoids of 40.88 µg/mg dry wt. with 62% more from the control condition, inferring that, carotenogenesis were induced greater than reduced nutrient. The ANOVA showed a significant R 2 value (0.9980), model F-value (380.07) and probability (0.0001), with insignificant lack of fit. The results suggested that there was no significant interactions betwe...

Journal of Microbiology and Biotechnology Research, 2017

The blue-green alga, Nostochopsis lobatus Wood em. Geitler, NCBI accession no. KJ562183.1 consist... more The blue-green alga, Nostochopsis lobatus Wood em. Geitler, NCBI accession no. KJ562183.1 consists of a dark green colony with a mucilaginous sheath, attached on rocks of flowing stream of Panna, Madhya Pradesh, India was the habitat and isolated. Scattered and lateral branches with stretching parallel and upward to the main filament in a polysaccharide sheath were the unique descriptive characteristics. The study of the bioactive compounds showed a high and comparable amount of sugar, pigments, chlorophyll, carotenoids, phycocyanin, phycoerythin, allophycocyanin including high soluble proteins and ammonia excretion in culture conditions. The 16S rRNA sequence was compared with the retrieved cultures from NCBI GenBank database. Phylogenetic analysis was largely consistent which was obtained from 16S rRNA gene sequence analysis. Phylogenetic tree was constructed by neighbor joining and the findings indicate that the genus Nostochopsis lobatus is distantly related to the genus Fischer...

Journal of Microbiology and Biotechnology Research, 2017

In the present study, ten (10) cyanobacterial strains belonging to (04) genera from different geo... more In the present study, ten (10) cyanobacterial strains belonging to (04) genera from different geographical habitats of Loktak Lake were morphologically identified upto genus/ species level and RAPD-PCR analysis using six (06) sets of random primers was also carried out to reveal their phylogenetic relationship and genetic diversity. The electrophoretic patterns for 10 cyanobacterial strains (03 Phormidium strains, 05 Nostoc strains, 01 Anabaena strain and 01 Calothrix strain) were used for molecular analysis using the RAPD technique. Unique banding patterns were observed from all tested cyanobacterial species and their molecular weights of each band were used to calculate their genetic distance among them. The bands generated through RAPD techniques were subjected to similarity matrix and similarity co-efficient ranged from 0.08 to 0.67. The cluster for Phormidium sp. (BTA-52) and Phormidium sp. (BTA-75) showed 66.7% similarity. Anabaena sp. (BTA-964) and Phormidium sp. (BTA-75) whi...

Journal of Microbiology and Biotechnology Research, 2017

Five non-heterocystous filamentous cyanobacteria were isolated from Indo-Burma Biodiversity Hotsp... more Five non-heterocystous filamentous cyanobacteria were isolated from Indo-Burma Biodiversity Hotspot namely, Phormidium animale BTA 258 (P1), Leptolyngbya sp. BTA 287 (L1), Leptolyngbya sp. BTA 477 (L2), Leptolyngbya tenerrima BTA 766 (L3) and Phormidium tenue BTA 803 (P2). Fatty acids were profiled and compared with cyanobacterial fatty acids reported from Western Ghats. Cyanobacterial SFA% and MUFA% of Indo-Burma region were comparatively less as compared to Western Ghats with highest shown by heterocystous cyanobacteria (41- 98% total fatty acids). Indo Burma cyanobacterial isolates present more MUFA% in average as compared to PUFA%. PUFA% were high in P1 (11.20%) and P2 (11.36%) compared to non-heterocystous groups of both the hotspot. Butyric acid (C4:0) was significant as specific chemotaxonomic marker for the Phormidium animale and Leptolyngbya sp. of Indo-Burma region. High amount of C13:0, C14:0, C14:1, C15:0, C20:1 and C20:2 were obtained in Phormidium and Leptolyngbya of I...

Annals of Biological Research, 2015

The present investigation deals with the cultural studies, pigment analysis, lipid profiling, tot... more The present investigation deals with the cultural studies, pigment analysis, lipid profiling, total sugar content, extracellular ammonium excretion and molecular characterization of cyanobacterium Gloeotrichia ghosei BTA 9020 (NCBI accession no. KM435243). Initially isolate was identified and grouped on the basis of morphological features followed by chemotaxonomical and molecular attributes. The study revealed that investigated organism produced good amount of secondary metabolites in culture conditions during decline phase of growth; unsaturated fatty acid, namely; palmitic acid C16:0 was found highest (25.1%) followed by linolelaidic acid C18:2n6t (21.9%). The 16S rRNA sequence was compared with the retrieved cultures from NCBI GenBank database. Phylogenetic analysis was largely consistent which was obtained from 16S rRNA gene sequence analysis. Phylogenetic trees were constructed by UPGMA, maximum-parsimony and neighbour joining and the findings indicate that the genus Gloeotr...

Annals of Biological Research, 2015

In the present study, the morphology and genetic diversity of ten (10) potential cyanobacterial s... more In the present study, the morphology and genetic diversity of ten (10) potential cyanobacterial strains isolated from fresh water habitats of Loktak Lake was investigated by Restriction Fragment Length Polymorphism of 16S rRNA genes using four different enzymes viz., HinfI, AluI, EcoRI and TaqI. These enzymes digested the 16S rRNA PCR products and yielded different profiles. These strains showed high phycobili proteins content, extracellular ammonium excretion and nitrogenase activity when preliminary screening was conducted. The strains Nostoc spp. (BTA-60, 61), Nostoc commune (BTA-67) and Nostocmuscorum(BTA-950) were similar and delineated from the rest by the enzymes EcoRI and AluI. Other digests which characterized Calothrix sp. BTA-73 as a distinct taxonomic group from the rest was catalyzed by the enzymes EcoRI and AluI. On the basis of the genetic polymorphism band, HinfI, TaqI and AluI were also able to discriminate Anabaena sp. (BTA-964)from the other cyanobacterial strains...

Journal of Applied Biology & Biotechnology, 2015

Thirty eight unialgal non-heterocystous filamentous cyanobacteria were isolated from rice fields ... more Thirty eight unialgal non-heterocystous filamentous cyanobacteria were isolated from rice fields of Manipur, India; cultured as unialgal, deposited to the national repository of cyanobacteria and microalgae and obtained accession number. All these strains were screened and investigated for the production of total lipid and commercially viable fatty acids in culture condition. Equal amount of total lipid (3%) was produced by Limnothrix vacuolifera BTA05, Plectonema boryanum BTA16, Plectonema nostocorum BTA47, Lyngbya laxespiralis BTA85 and Lyngbya norgardhii BTA184 in exponential growth phase. The commercially viable fatty acids, namely; palmitic acid (C16:0), palmitoleic acid (C16:1), oleic acid (C18:1n9c), linoleic acid (C18:2n6c) and γlinolenic acid (C18:3n6) were focused in present study. The investigation revealed that Plectonema notatum BTA88 yielded high content of palmitic acid (27.9%); Oscillatoria agardhii BTA170 of palmitoleic acid (8.90%); Lyngbya martensiana BTA640 of oleic acid (56.2%); Phormidium faveolarum BTA20 (11.8%) in linoleic acid and Phormidium boryanum BTA16 of γlinolenic acid (8.82%). These organism were considered as the potential candidates for fatty acids profiling, however palmitic acid C16:0 was common and recorded in all 38 examined strains.

Journal of Applied Biology & Biotechnology, 2015

In the present study, cyanobacterial strains isolated from Loktak Lake were investigated for thei... more In the present study, cyanobacterial strains isolated from Loktak Lake were investigated for their possibility of increasing the content of phycobiliproteins and ammonium excretion under different levels of pH. At pH 5.0, maximum amount of PE was observed in Nostoc sp. BTA-61 (111.10±9.43 µgmg-1). Maximum PC was expressed in Phormidium sp. BTA-1048 (155.17±10.15 µgmg-1). APC content was observed maximum in Nostoc commune BTA-67 (81.45±7.68 µgmg-1). At pH 6.0, high PE was observed in Nostoc sp. BTA-61 (125.11±11.60 µgmg-1). PC was maximum in Phormidium sp. BTA-1048 (168.15±9.58 µgmg-1). APC was highest in Nostoc commune BTA-67 (91.41±2.88 µgmg-1). Regarding at pH 7.0, PE was found in high quantity in Nostoc sp. BTA-61 (127.91±14.91 µgmg-1). PC was maximum in Nostoc commune BTA-67 (128.52±5.66 µgmg-1). APC was highest in Nostoc commune BTA-67 (96.40±3.52 µgmg-1). pH 8.0 showed high PE content in Nostoc sp. BTA-61 (115.75±6.27 µgmg-1). PC was highest in Phormidium sp. BTA-1048 (160.09±10.56 µgmg-1). APC was maximum in Nostoc commune BTA-67 (84.15±5.30 µgmg-1). pH 9.0 showed maximum PE content in Nostoc sp. BTA-61 (95.39±4.87 µgmg-1). PC was high in Phormidium sp. BTA-1048 (148.13±9.20 µgmg-1). APC was highest in Nostoc commune BTA-67 (77.43±5.78 µgmg-1). PE, PC and APC concentration was significantly higher (LSD test, p<0.05) in pH 7.0 when compared with the other pH 5.0, 6.0, 8.0 and 9.0. In this study, maximum ammonium excretion was observed in Nostoc muscorum BTA-950 in all the different pH and minimum in Anabaena sp. BTA-964. The findings indicates that these cyanobacterial strains may be proved as promising microorganism for the phycobiliproteins production and ammonium excretion which could be exploited biotechnologically for the benefit of commercial applications.

In the present investigation, nine (09) unicellular microgreen algae of freshwater habitats of Ma... more In the present investigation, nine (09) unicellular microgreen algae of freshwater habitats of Manipur were characterized for total lipid content and fatty acid composition. Nile red fluorescence indicated more number of lipid bodies at stationary growth phase. Maximum lipid content was observed in Chlorella sp. (BTA-3110). Profiling of fatty acid composition showed that palmitic acid (16:0) and linolenic acid (C18:3n3) were the predominant components. Chlorococcum sp. (BTA-3112) and Korshikoviella sp. (BTA-3019) showed high palmitic acid as 54.25% and 49.68% respectively. Arachidic acid (C20:0) was maximum in Chlorella sp. (BTA-3110) as 21.57%. Among the polyunsaturated fatty acid (PUFA), linolenic acid (C18:3n3) was maximum in Chlorella sp. (BTA-3063) as 37.37%. High amount of linoleic acid (C18:2n6) was observed in Chlorella sp. (BTA-3101) as 12.62%. One of the pharmaceutically potential component, eicosapentaenoic acid (C20:5n3) occurred maximum amount in Chlorella sp. (BTA-308...

Carotenoids are commercially used as natural colourant, antioxidant and as a vitamin A precursors... more Carotenoids are commercially used as natural colourant, antioxidant and as a vitamin A precursors. In the present investigation, critical factors for high total carotenoid yield from the effect of stress created by high pH, excess NaNO3 and K2HPO4 were investigated by using single factor experiment and later optimization were carried out by using Box-Behnken experimental design from Leptolyngbya sp. BTA 287 belonging to Indo-Burma Biodiversity hot spot. This strain was found to produce maximum total carotenoids at pH 9, NaNO3 concentration of 7.50 mg/ml and K2HPO4 concentration at 0.20 mg ml -1 in BG-11 medium, yielding highest total carotenoids of 40.88 µg/mg dry wt. with 62% more from the control condition, inferring that, carotenogenesis were induced greater than reduced nutrient. The ANOVA showed a significant R 2 value (0.9980), model F-value (380.07) and probability (0.0001), with insignificant lack of fit. The results suggested that there was no significant interactions betwe...

Journal of Microbiology and Biotechnology Research, 2017

The blue-green alga, Nostochopsis lobatus Wood em. Geitler, NCBI accession no. KJ562183.1 consist... more The blue-green alga, Nostochopsis lobatus Wood em. Geitler, NCBI accession no. KJ562183.1 consists of a dark green colony with a mucilaginous sheath, attached on rocks of flowing stream of Panna, Madhya Pradesh, India was the habitat and isolated. Scattered and lateral branches with stretching parallel and upward to the main filament in a polysaccharide sheath were the unique descriptive characteristics. The study of the bioactive compounds showed a high and comparable amount of sugar, pigments, chlorophyll, carotenoids, phycocyanin, phycoerythin, allophycocyanin including high soluble proteins and ammonia excretion in culture conditions. The 16S rRNA sequence was compared with the retrieved cultures from NCBI GenBank database. Phylogenetic analysis was largely consistent which was obtained from 16S rRNA gene sequence analysis. Phylogenetic tree was constructed by neighbor joining and the findings indicate that the genus Nostochopsis lobatus is distantly related to the genus Fischer...

Journal of Microbiology and Biotechnology Research, 2017

In the present study, ten (10) cyanobacterial strains belonging to (04) genera from different geo... more In the present study, ten (10) cyanobacterial strains belonging to (04) genera from different geographical habitats of Loktak Lake were morphologically identified upto genus/ species level and RAPD-PCR analysis using six (06) sets of random primers was also carried out to reveal their phylogenetic relationship and genetic diversity. The electrophoretic patterns for 10 cyanobacterial strains (03 Phormidium strains, 05 Nostoc strains, 01 Anabaena strain and 01 Calothrix strain) were used for molecular analysis using the RAPD technique. Unique banding patterns were observed from all tested cyanobacterial species and their molecular weights of each band were used to calculate their genetic distance among them. The bands generated through RAPD techniques were subjected to similarity matrix and similarity co-efficient ranged from 0.08 to 0.67. The cluster for Phormidium sp. (BTA-52) and Phormidium sp. (BTA-75) showed 66.7% similarity. Anabaena sp. (BTA-964) and Phormidium sp. (BTA-75) whi...

Journal of Microbiology and Biotechnology Research, 2017

Five non-heterocystous filamentous cyanobacteria were isolated from Indo-Burma Biodiversity Hotsp... more Five non-heterocystous filamentous cyanobacteria were isolated from Indo-Burma Biodiversity Hotspot namely, Phormidium animale BTA 258 (P1), Leptolyngbya sp. BTA 287 (L1), Leptolyngbya sp. BTA 477 (L2), Leptolyngbya tenerrima BTA 766 (L3) and Phormidium tenue BTA 803 (P2). Fatty acids were profiled and compared with cyanobacterial fatty acids reported from Western Ghats. Cyanobacterial SFA% and MUFA% of Indo-Burma region were comparatively less as compared to Western Ghats with highest shown by heterocystous cyanobacteria (41- 98% total fatty acids). Indo Burma cyanobacterial isolates present more MUFA% in average as compared to PUFA%. PUFA% were high in P1 (11.20%) and P2 (11.36%) compared to non-heterocystous groups of both the hotspot. Butyric acid (C4:0) was significant as specific chemotaxonomic marker for the Phormidium animale and Leptolyngbya sp. of Indo-Burma region. High amount of C13:0, C14:0, C14:1, C15:0, C20:1 and C20:2 were obtained in Phormidium and Leptolyngbya of I...

Annals of Biological Research, 2015

The present investigation deals with the cultural studies, pigment analysis, lipid profiling, tot... more The present investigation deals with the cultural studies, pigment analysis, lipid profiling, total sugar content, extracellular ammonium excretion and molecular characterization of cyanobacterium Gloeotrichia ghosei BTA 9020 (NCBI accession no. KM435243). Initially isolate was identified and grouped on the basis of morphological features followed by chemotaxonomical and molecular attributes. The study revealed that investigated organism produced good amount of secondary metabolites in culture conditions during decline phase of growth; unsaturated fatty acid, namely; palmitic acid C16:0 was found highest (25.1%) followed by linolelaidic acid C18:2n6t (21.9%). The 16S rRNA sequence was compared with the retrieved cultures from NCBI GenBank database. Phylogenetic analysis was largely consistent which was obtained from 16S rRNA gene sequence analysis. Phylogenetic trees were constructed by UPGMA, maximum-parsimony and neighbour joining and the findings indicate that the genus Gloeotr...

Annals of Biological Research, 2015

In the present study, the morphology and genetic diversity of ten (10) potential cyanobacterial s... more In the present study, the morphology and genetic diversity of ten (10) potential cyanobacterial strains isolated from fresh water habitats of Loktak Lake was investigated by Restriction Fragment Length Polymorphism of 16S rRNA genes using four different enzymes viz., HinfI, AluI, EcoRI and TaqI. These enzymes digested the 16S rRNA PCR products and yielded different profiles. These strains showed high phycobili proteins content, extracellular ammonium excretion and nitrogenase activity when preliminary screening was conducted. The strains Nostoc spp. (BTA-60, 61), Nostoc commune (BTA-67) and Nostocmuscorum(BTA-950) were similar and delineated from the rest by the enzymes EcoRI and AluI. Other digests which characterized Calothrix sp. BTA-73 as a distinct taxonomic group from the rest was catalyzed by the enzymes EcoRI and AluI. On the basis of the genetic polymorphism band, HinfI, TaqI and AluI were also able to discriminate Anabaena sp. (BTA-964)from the other cyanobacterial strains...

Journal of Applied Biology & Biotechnology, 2015

Thirty eight unialgal non-heterocystous filamentous cyanobacteria were isolated from rice fields ... more Thirty eight unialgal non-heterocystous filamentous cyanobacteria were isolated from rice fields of Manipur, India; cultured as unialgal, deposited to the national repository of cyanobacteria and microalgae and obtained accession number. All these strains were screened and investigated for the production of total lipid and commercially viable fatty acids in culture condition. Equal amount of total lipid (3%) was produced by Limnothrix vacuolifera BTA05, Plectonema boryanum BTA16, Plectonema nostocorum BTA47, Lyngbya laxespiralis BTA85 and Lyngbya norgardhii BTA184 in exponential growth phase. The commercially viable fatty acids, namely; palmitic acid (C16:0), palmitoleic acid (C16:1), oleic acid (C18:1n9c), linoleic acid (C18:2n6c) and γlinolenic acid (C18:3n6) were focused in present study. The investigation revealed that Plectonema notatum BTA88 yielded high content of palmitic acid (27.9%); Oscillatoria agardhii BTA170 of palmitoleic acid (8.90%); Lyngbya martensiana BTA640 of oleic acid (56.2%); Phormidium faveolarum BTA20 (11.8%) in linoleic acid and Phormidium boryanum BTA16 of γlinolenic acid (8.82%). These organism were considered as the potential candidates for fatty acids profiling, however palmitic acid C16:0 was common and recorded in all 38 examined strains.

Journal of Applied Biology & Biotechnology, 2015

In the present study, cyanobacterial strains isolated from Loktak Lake were investigated for thei... more In the present study, cyanobacterial strains isolated from Loktak Lake were investigated for their possibility of increasing the content of phycobiliproteins and ammonium excretion under different levels of pH. At pH 5.0, maximum amount of PE was observed in Nostoc sp. BTA-61 (111.10±9.43 µgmg-1). Maximum PC was expressed in Phormidium sp. BTA-1048 (155.17±10.15 µgmg-1). APC content was observed maximum in Nostoc commune BTA-67 (81.45±7.68 µgmg-1). At pH 6.0, high PE was observed in Nostoc sp. BTA-61 (125.11±11.60 µgmg-1). PC was maximum in Phormidium sp. BTA-1048 (168.15±9.58 µgmg-1). APC was highest in Nostoc commune BTA-67 (91.41±2.88 µgmg-1). Regarding at pH 7.0, PE was found in high quantity in Nostoc sp. BTA-61 (127.91±14.91 µgmg-1). PC was maximum in Nostoc commune BTA-67 (128.52±5.66 µgmg-1). APC was highest in Nostoc commune BTA-67 (96.40±3.52 µgmg-1). pH 8.0 showed high PE content in Nostoc sp. BTA-61 (115.75±6.27 µgmg-1). PC was highest in Phormidium sp. BTA-1048 (160.09±10.56 µgmg-1). APC was maximum in Nostoc commune BTA-67 (84.15±5.30 µgmg-1). pH 9.0 showed maximum PE content in Nostoc sp. BTA-61 (95.39±4.87 µgmg-1). PC was high in Phormidium sp. BTA-1048 (148.13±9.20 µgmg-1). APC was highest in Nostoc commune BTA-67 (77.43±5.78 µgmg-1). PE, PC and APC concentration was significantly higher (LSD test, p<0.05) in pH 7.0 when compared with the other pH 5.0, 6.0, 8.0 and 9.0. In this study, maximum ammonium excretion was observed in Nostoc muscorum BTA-950 in all the different pH and minimum in Anabaena sp. BTA-964. The findings indicates that these cyanobacterial strains may be proved as promising microorganism for the phycobiliproteins production and ammonium excretion which could be exploited biotechnologically for the benefit of commercial applications.

In the present investigation, nine (09) unicellular microgreen algae of freshwater habitats of Ma... more In the present investigation, nine (09) unicellular microgreen algae of freshwater habitats of Manipur were characterized for total lipid content and fatty acid composition. Nile red fluorescence indicated more number of lipid bodies at stationary growth phase. Maximum lipid content was observed in Chlorella sp. (BTA-3110). Profiling of fatty acid composition showed that palmitic acid (16:0) and linolenic acid (C18:3n3) were the predominant components. Chlorococcum sp. (BTA-3112) and Korshikoviella sp. (BTA-3019) showed high palmitic acid as 54.25% and 49.68% respectively. Arachidic acid (C20:0) was maximum in Chlorella sp. (BTA-3110) as 21.57%. Among the polyunsaturated fatty acid (PUFA), linolenic acid (C18:3n3) was maximum in Chlorella sp. (BTA-3063) as 37.37%. High amount of linoleic acid (C18:2n6) was observed in Chlorella sp. (BTA-3101) as 12.62%. One of the pharmaceutically potential component, eicosapentaenoic acid (C20:5n3) occurred maximum amount in Chlorella sp. (BTA-308...