Indu Bhushan - Academia.edu (original) (raw)
Papers by Indu Bhushan
International Journal of Biological Macromolecules, Jun 1, 2017
Heparin is a member of the glycosaminoglycan (GAG) family composed of glucosamine and uronic acid... more Heparin is a member of the glycosaminoglycan (GAG) family composed of glucosamine and uronic acid units containing O-sulfo, N-acetyl and N-sulfo groups, which are alternating in the chain and linked by 1→4 manner. It is a naturally occurring anticoagulant that prevents the formation of clots and their growth within blood. Certain low molecular weight heparins (LMWHs) are considered as better therapeutic agents than natural heparin because of the reduced side effects and smaller risk of bleeding. LMWHs can be produced from heparin by chemical or enzymatic depolymerizations. Heparinases catalyze the cleavage of glycosidic linkage between amino sugars and uronic acids in heparin. There are three kinds of heparinases which are frequently used for depolymerization of heparin. Despite wide range of applications of heparinases in health care, their use still has been hampered due to poor stability and high cost. To overcome this problem heparinases are recommended for immobilization to reduce the cost of product and enhance stability. Heparinases have been successfully immobilized using various methods and supports, mostly for deheparinization of blood through extracorporeal devices. The focus of this review is to present the current status of heparinase immobilization including various supports and methods used, stability and applications.
International Journal of Biological Macromolecules, 2017
Heparin is a member of the glycosaminoglycan (GAG) family composed of glucosamine and uronic acid... more Heparin is a member of the glycosaminoglycan (GAG) family composed of glucosamine and uronic acid units containing O-sulfo, N-acetyl and N-sulfo groups, which are alternating in the chain and linked by 1→4 manner. It is a naturally occurring anticoagulant that prevents the formation of clots and their growth within blood. Certain low molecular weight heparins (LMWHs) are considered as better therapeutic agents than natural heparin because of the reduced side effects and smaller risk of bleeding. LMWHs can be produced from heparin by chemical or enzymatic depolymerizations. Heparinases catalyze the cleavage of glycosidic linkage between amino sugars and uronic acids in heparin. There are three kinds of heparinases which are frequently used for depolymerization of heparin. Despite wide range of applications of heparinases in health care, their use still has been hampered due to poor stability and high cost. To overcome this problem heparinases are recommended for immobilization to reduce the cost of product and enhance stability. Heparinases have been successfully immobilized using various methods and supports, mostly for deheparinization of blood through extracorporeal devices. The focus of this review is to present the current status of heparinase immobilization including various supports and methods used, stability and applications.
Environment Conservation Journal, Dec 20, 2012
Banganga is the first major station for a yatries when they undertake their on-foot journey from ... more Banganga is the first major station for a yatries when they undertake their on-foot journey from Katra to Bhawan (Shri Mata Vaishno Devi). The pilgrims reach a small bridge under which flows Banganga, the legendary river associated with the miracles and legends of Mata Vaishno Devi. There is water in the river all through the year. Various physico-chemical and biological parameters were taken into consideration during the course of study, these includes colour, temperature, DO, BOD, COD, pH, total hardness, total suspended solids and Total dissolved solids. Based on the results of this study it has concluded that slight increase of pollutants in downstream of the river was observed in comparison to the upstream, hence it is an alarming position of increasing pollution load in the river.
Sustainability, May 31, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Bioresource Technology, Dec 1, 2018
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service... more This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Enantioselective resolution of 2-arylpropionic acid derivatives employing immobilization of lipase from Bacillus subtilis strain Kakrayal_1 (BSK-L)
New Biotechnology, Oct 1, 2018
Future Microbiology, Dec 1, 2022
The microbial communities are an indispensable part of the human defense system and coexist with ... more The microbial communities are an indispensable part of the human defense system and coexist with humans as symbionts, contributing to the metabolic functions and immune defense against pathogens. An ecologically stable vaginal microbiota is dominated by Lactobacillus species, which plays an important role in the prevention of genital infections by controlling the vaginal pH, reducing glycogen to lactic acid, and stimulating bacteriocins and hydrogen peroxide. In contrast, an abnormal vaginal microbial composition is associated with an increased risk of bacterial vaginosis, trichomoniasis, sexually transmitted diseases, preterm labor and other birth defects. This microbial diversity is affected by race, ethnicity, pregnancy, hormonal changes, sexual activities, hygiene practices and other conditions. In the present review, we discuss the changes in the microbial community of the vaginal region at different stages of a female's life cycle and its influence on her reproductive health and pathological conditions.
3 biotech, Nov 19, 2018
The present study was conducted to purify lipase from indigenous Bacillus subtilis strain Kakraya... more The present study was conducted to purify lipase from indigenous Bacillus subtilis strain Kakrayal_1 (BSK-L) for enantioselective resolution of racemic-ketoprofen. The production of lipase (BSK-L) was optimized using Plackett-Burman and central composite design of response surface methodology (RSM). The optimized media containing olive oil (3.5%), MnSO 4 (8 mM), CaCl 2 (5 mM), peptone (20 g/l), pH (8), agitation (180 rpm) and temperature (37 °C) resulted in maximum lipase production of 7500 U/g of cell biomass. The lipase was purified using sequential method to an overall purification fold of 13% with 20% recovery, 882 U/mg specific activity and a molecular weight of 45 kDa. Optimal pH and temperature of purified lipase were found to be 8 and 37 °C, respectively. Furthermore, BSK-L displayed good stability with various organic solvents, surfactants and metal ions. K m and V max values of lipase were observed to be 2.2 mM and 6.67 mmoles of product formed/ min/mg, respectively. The racemic ketoprofen butyl ester was hydrolyzed using lipase with 49% conversion efficiency and 69% enantiomeric excess (ee) which was superior to the commercially procured lipase (Candida antarctica lipase). Thus, this enzyme could be considered as a promising candidate for the pharmaceutical industry.
Biofuel and biorefinery technologies, Jul 20, 2018
Solid-state fermentation (SSF) is the fermentation process which occurs on a solid surface in the... more Solid-state fermentation (SSF) is the fermentation process which occurs on a solid surface in the absence of “free” water, where the moisture is absorbed to the solid matrix. SSF is gaining an advantageous edge over other fermentation techniques due to its less complexity and more proximity to the normal environment of many microorganisms. On the other hand, a difficulty arises while estimating the biomass concentration in solid-state fermentation. Various factors like direct product application, the increased concentration of the product, less cost of production, and reduced energy requirement are responsible in making SSF as one of the potent technologies for various enzyme productions as seen in case of cellulase, tannase, and lipase. Improvisation of cellulase production in solid-state fermentation can be achieved to a greater extent by making use of varying degrees of substrates which are lignocellulosic in nature, the implicated microorganisms, culture, and process parameters like moisture content and water activity, nutrients diffusion, size of substrate particle, pH, temperature, surfactants, and bioreactor designs. Submerged fermentation whereas holds a different place in terms of various types of fermentations as it has only one major problem related to the oxygen transfer to microorganisms which in turn depends on the configuration, size, and the agitation/aeration system used in the reactor. In order to characterize oxygen transfer, a parameter is known as K La (oxygen transfer coefficient) whose value gives the estimation that how much of the oxygen is transferred by the equipment independent of the reactor volume and hence, for scale-up studies, it becomes an important parameter. In case of antioxidants production using SSF, it was observed that pomace tends to increase the antioxidant activity convergent with an increase in activity of β-glucosidase. Different studies tend to show that P. floridensis as an important organism used during the production of lingo cellulolytic enzyme and consecutive advancement in in vitro digestibility of wheat straw has been carried out to a larger extent.
Journal of Chemical Technology & Biotechnology, Sep 2, 2010
... Correspondence: Indu Bhushan, Shri ... The ingredients of the media were dissolved in 800 mL ... more ... Correspondence: Indu Bhushan, Shri ... The ingredients of the media were dissolved in 800 mL of distilled water, pH was adjusted to 7.0 using 0.1 mol L −1 NaOH or 0.1N HCl and the final volume was made up to 1 L. For preparation of solid media, 2% agar was added to the ...
Environmental Science and Pollution Research, Aug 4, 2021
The industrial revolution has marked a strong impact on financial upgradation of several countrie... more The industrial revolution has marked a strong impact on financial upgradation of several countries, and increase in the industrial establishment globally has direct impact on environment because of the release of unwanted product in air and inside the water bodies. The use of dyes has increased tremendously in various industries ranging from food, leather, textile, paper, cosmetic, pharmaceuticals, etc. The problem has emerged due to disposing of the dyes in the open environment, and mostly it is disposed along with the industrial wastes into the water bodies, which becomes harmful for animals, aquatic life and human health. This review highlights the role of the nanoparticles particularly biosynthesized nanoparticles for eliminating the dyes from the industrial wastewater. There are several methods for the synthesis of nanoparticle including physical, chemical and green synthesis of nanoparticles commonly known as biological method. Among all, the biological method is considered as the rapid, easy, eco-friendly and is being performed at mild conditions. The uses of nanoparticles for removal of dyes from water minimize the hazardous impact and thus considered to be the best approach as far as water quality and safety of environment is concerned.
Microbial Pathogenesis, Nov 1, 2019
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Life, Nov 13, 2021
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Annals of biotechnology, Feb 19, 2018
Alzheimer's disease is an unavoidable neurological disorder in which the death of brain cells cau... more Alzheimer's disease is an unavoidable neurological disorder in which the death of brain cells causes memory loss and cognitive decline and ultimate dementia. It is the most common cause of dementia in people of 65 years and older. It affects 10% of people over the age of 65 and 50% over the age of 85 years. Approximately 4million Alzheimer's patients in the United States (U.S.) and the annual treatment costs = $100 billion. It is the fourth leading cause of death in the United States and is becoming prevalent in many other countries. The total brain size shrinks with Alzheimer's-the tissue has progressively fewer nerve cells and connections. As such there is no known cure for Alzheimer's disease the death of brain cells in the dementia cannot be halted or reversed. Along with an aim to improve research in to prevention and treatment, the goals of the plan also include measures for present interventions. To help people suffering expand supports for people with Alzheimer's disease and their families, and enhance public awareness and engagement and expand your support towards them. Enhance care quality and efficiency. There are no disease-modifying drugs available for Alzheimer's disease but some options may reduce its symptoms and help improve quality of life and thereby help the patients to some extent. There are four drugs in a class called cholinesterase inhibitor approved for symptomatic relief in the US i.e., Donepezil (brand name Aricept), Alantamine (Reminyl), Rivastigmine and Tacrine (Cognex). A different kind of drug, memantine (Namenda), an N-methyl-D-aspartate (NMDA) receptor antagonist, may also be used, alone or in combination with a cholinesterase inhibitor. As with other types of dementia and neurodegenerative disease, a major part of therapy for patients with Alzheimer's comes from the support given by healthcare workers to provide dementia quality-of-life care, which becomes more important as needs increase with declining independence and increasing dependence.
Abstract book of the 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology" PLAMIC2020, 2020
Lipases are a multipurpose enzyme that holds a significant position in industrial applications du... more Lipases are a multipurpose enzyme that holds a significant position in industrial applications due to its ability to catalyse a large number of reactions such as hydrolysis, esterification, interesterification, transesterification which makes it a potential candidate. It is also used for the separation of chiral drugs from the racemic mixture and this property of lipase is considered very important in pharmaceutical industries for the synthesis of enantiopure bioactive molecules. Assuming the tremendous importance of lipases, as stereoselective biocatalysts, in pharmaceuticals and various other commercial applications, industrial enzymologists have been forced to search for those microorganisms which are able to produce novel biocatalysts at reasonably high yield. In the present study microbial lipase was isolated from the water sample of pond at Katra, Jammu and Kashmir (India). This enzyme has shown wide specificity and higher enantioselectivity, which make it pharmaceutical impor...
Journal of Materials Science, Sep 1, 2022
Sustainability
The present study focus on biosynthesis of stable silver nanoparticles (AgNPs) and zinc oxide nan... more The present study focus on biosynthesis of stable silver nanoparticles (AgNPs) and zinc oxide nanoparticles (ZnO NPs) from the leaf and stem extract of a therapeutic plant Carissa opaca. The visual observation, Fourier Transformed Infrared Spectroscopy (FTIR), Inductively Coupled Plasma analysis (ICP), High Resolution Transmission Electron Microscopy (HRTEM), and Scanning Electron Microscopy-Energy Dispersive Spectroscopy (SEM-EDS) were used to characterize and confirm the synthesized AgNPs and ZnO NPs. Afterwards; the synthesized nanoparticles were used to analyze their antimicrobial activity via in-vitro disk diffusion method against Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis, Aspergillus niger, and Candida albican. Both the nanoparticles showed maximum zone of inhibition against Pseudomonas aeruginosa (bacterial strain), whereas in the case of fungi, higher zone of inhibition was observed using ZnONPs against Candida albican and AgNPs against Aspergillus nig...
Environment Conservation Journal
Banganga is the first major station for a yatries when they undertake their on-foot journey from ... more Banganga is the first major station for a yatries when they undertake their on-foot journey from Katra to Bhawan (Shri Mata Vaishno Devi). The pilgrims reach a small bridge under which flows Banganga, the legendary river associated with the miracles and legends of Mata Vaishno Devi. There is water in the river all through the year. Various physico-chemical and biological parameters were taken into consideration during the course of study, these includes colour, temperature, DO, BOD, COD, pH, total hardness, total suspended solids and Total dissolved solids. Based on the results of this study it has concluded that slight increase of pollutants in downstream of the river was observed in comparison to the upstream, hence it is an alarming position of increasing pollution load in the river.
Future Microbiology
The microbial communities are an indispensable part of the human defense system and coexist with ... more The microbial communities are an indispensable part of the human defense system and coexist with humans as symbionts, contributing to the metabolic functions and immune defense against pathogens. An ecologically stable vaginal microbiota is dominated by Lactobacillus species, which plays an important role in the prevention of genital infections by controlling the vaginal pH, reducing glycogen to lactic acid, and stimulating bacteriocins and hydrogen peroxide. In contrast, an abnormal vaginal microbial composition is associated with an increased risk of bacterial vaginosis, trichomoniasis, sexually transmitted diseases, preterm labor and other birth defects. This microbial diversity is affected by race, ethnicity, pregnancy, hormonal changes, sexual activities, hygiene practices and other conditions. In the present review, we discuss the changes in the microbial community of the vaginal region at different stages of a female's life cycle and its influence on her reproductive heal...
Life, 2021
There is a unique microbial community in the female lower genital tract known as the vaginal micr... more There is a unique microbial community in the female lower genital tract known as the vaginal microbiota, which varies in composition and density and provides significant benefits during pregnancy, reproductive cyclicity, healthy newborn delivery, protection from preterm birth, infections such as UTIs, bacterial vaginosis, and so on, and improves the efficacy of treatments for vaginal cancers. Methods: It is necessary to know how the vaginal microbiome is composed in order to make an accurate diagnosis of the diseases listed above. A microbiome’s members are difficult to classify, and the way microbial communities function and influence host–pathogen interactions are difficult to understand. More and more metagenomic studies are able to unravel such complexities due to advances in high-throughput sequencing and bioinformatics. When it comes to vaginal microbiota research, we’ll be looking at the use of modern techniques and strategies that can be used to investigate variations in vag...
International Journal of Biological Macromolecules, Jun 1, 2017
Heparin is a member of the glycosaminoglycan (GAG) family composed of glucosamine and uronic acid... more Heparin is a member of the glycosaminoglycan (GAG) family composed of glucosamine and uronic acid units containing O-sulfo, N-acetyl and N-sulfo groups, which are alternating in the chain and linked by 1→4 manner. It is a naturally occurring anticoagulant that prevents the formation of clots and their growth within blood. Certain low molecular weight heparins (LMWHs) are considered as better therapeutic agents than natural heparin because of the reduced side effects and smaller risk of bleeding. LMWHs can be produced from heparin by chemical or enzymatic depolymerizations. Heparinases catalyze the cleavage of glycosidic linkage between amino sugars and uronic acids in heparin. There are three kinds of heparinases which are frequently used for depolymerization of heparin. Despite wide range of applications of heparinases in health care, their use still has been hampered due to poor stability and high cost. To overcome this problem heparinases are recommended for immobilization to reduce the cost of product and enhance stability. Heparinases have been successfully immobilized using various methods and supports, mostly for deheparinization of blood through extracorporeal devices. The focus of this review is to present the current status of heparinase immobilization including various supports and methods used, stability and applications.
International Journal of Biological Macromolecules, 2017
Heparin is a member of the glycosaminoglycan (GAG) family composed of glucosamine and uronic acid... more Heparin is a member of the glycosaminoglycan (GAG) family composed of glucosamine and uronic acid units containing O-sulfo, N-acetyl and N-sulfo groups, which are alternating in the chain and linked by 1→4 manner. It is a naturally occurring anticoagulant that prevents the formation of clots and their growth within blood. Certain low molecular weight heparins (LMWHs) are considered as better therapeutic agents than natural heparin because of the reduced side effects and smaller risk of bleeding. LMWHs can be produced from heparin by chemical or enzymatic depolymerizations. Heparinases catalyze the cleavage of glycosidic linkage between amino sugars and uronic acids in heparin. There are three kinds of heparinases which are frequently used for depolymerization of heparin. Despite wide range of applications of heparinases in health care, their use still has been hampered due to poor stability and high cost. To overcome this problem heparinases are recommended for immobilization to reduce the cost of product and enhance stability. Heparinases have been successfully immobilized using various methods and supports, mostly for deheparinization of blood through extracorporeal devices. The focus of this review is to present the current status of heparinase immobilization including various supports and methods used, stability and applications.
Environment Conservation Journal, Dec 20, 2012
Banganga is the first major station for a yatries when they undertake their on-foot journey from ... more Banganga is the first major station for a yatries when they undertake their on-foot journey from Katra to Bhawan (Shri Mata Vaishno Devi). The pilgrims reach a small bridge under which flows Banganga, the legendary river associated with the miracles and legends of Mata Vaishno Devi. There is water in the river all through the year. Various physico-chemical and biological parameters were taken into consideration during the course of study, these includes colour, temperature, DO, BOD, COD, pH, total hardness, total suspended solids and Total dissolved solids. Based on the results of this study it has concluded that slight increase of pollutants in downstream of the river was observed in comparison to the upstream, hence it is an alarming position of increasing pollution load in the river.
Sustainability, May 31, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Bioresource Technology, Dec 1, 2018
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service... more This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Enantioselective resolution of 2-arylpropionic acid derivatives employing immobilization of lipase from Bacillus subtilis strain Kakrayal_1 (BSK-L)
New Biotechnology, Oct 1, 2018
Future Microbiology, Dec 1, 2022
The microbial communities are an indispensable part of the human defense system and coexist with ... more The microbial communities are an indispensable part of the human defense system and coexist with humans as symbionts, contributing to the metabolic functions and immune defense against pathogens. An ecologically stable vaginal microbiota is dominated by Lactobacillus species, which plays an important role in the prevention of genital infections by controlling the vaginal pH, reducing glycogen to lactic acid, and stimulating bacteriocins and hydrogen peroxide. In contrast, an abnormal vaginal microbial composition is associated with an increased risk of bacterial vaginosis, trichomoniasis, sexually transmitted diseases, preterm labor and other birth defects. This microbial diversity is affected by race, ethnicity, pregnancy, hormonal changes, sexual activities, hygiene practices and other conditions. In the present review, we discuss the changes in the microbial community of the vaginal region at different stages of a female's life cycle and its influence on her reproductive health and pathological conditions.
3 biotech, Nov 19, 2018
The present study was conducted to purify lipase from indigenous Bacillus subtilis strain Kakraya... more The present study was conducted to purify lipase from indigenous Bacillus subtilis strain Kakrayal_1 (BSK-L) for enantioselective resolution of racemic-ketoprofen. The production of lipase (BSK-L) was optimized using Plackett-Burman and central composite design of response surface methodology (RSM). The optimized media containing olive oil (3.5%), MnSO 4 (8 mM), CaCl 2 (5 mM), peptone (20 g/l), pH (8), agitation (180 rpm) and temperature (37 °C) resulted in maximum lipase production of 7500 U/g of cell biomass. The lipase was purified using sequential method to an overall purification fold of 13% with 20% recovery, 882 U/mg specific activity and a molecular weight of 45 kDa. Optimal pH and temperature of purified lipase were found to be 8 and 37 °C, respectively. Furthermore, BSK-L displayed good stability with various organic solvents, surfactants and metal ions. K m and V max values of lipase were observed to be 2.2 mM and 6.67 mmoles of product formed/ min/mg, respectively. The racemic ketoprofen butyl ester was hydrolyzed using lipase with 49% conversion efficiency and 69% enantiomeric excess (ee) which was superior to the commercially procured lipase (Candida antarctica lipase). Thus, this enzyme could be considered as a promising candidate for the pharmaceutical industry.
Biofuel and biorefinery technologies, Jul 20, 2018
Solid-state fermentation (SSF) is the fermentation process which occurs on a solid surface in the... more Solid-state fermentation (SSF) is the fermentation process which occurs on a solid surface in the absence of “free” water, where the moisture is absorbed to the solid matrix. SSF is gaining an advantageous edge over other fermentation techniques due to its less complexity and more proximity to the normal environment of many microorganisms. On the other hand, a difficulty arises while estimating the biomass concentration in solid-state fermentation. Various factors like direct product application, the increased concentration of the product, less cost of production, and reduced energy requirement are responsible in making SSF as one of the potent technologies for various enzyme productions as seen in case of cellulase, tannase, and lipase. Improvisation of cellulase production in solid-state fermentation can be achieved to a greater extent by making use of varying degrees of substrates which are lignocellulosic in nature, the implicated microorganisms, culture, and process parameters like moisture content and water activity, nutrients diffusion, size of substrate particle, pH, temperature, surfactants, and bioreactor designs. Submerged fermentation whereas holds a different place in terms of various types of fermentations as it has only one major problem related to the oxygen transfer to microorganisms which in turn depends on the configuration, size, and the agitation/aeration system used in the reactor. In order to characterize oxygen transfer, a parameter is known as K La (oxygen transfer coefficient) whose value gives the estimation that how much of the oxygen is transferred by the equipment independent of the reactor volume and hence, for scale-up studies, it becomes an important parameter. In case of antioxidants production using SSF, it was observed that pomace tends to increase the antioxidant activity convergent with an increase in activity of β-glucosidase. Different studies tend to show that P. floridensis as an important organism used during the production of lingo cellulolytic enzyme and consecutive advancement in in vitro digestibility of wheat straw has been carried out to a larger extent.
Journal of Chemical Technology & Biotechnology, Sep 2, 2010
... Correspondence: Indu Bhushan, Shri ... The ingredients of the media were dissolved in 800 mL ... more ... Correspondence: Indu Bhushan, Shri ... The ingredients of the media were dissolved in 800 mL of distilled water, pH was adjusted to 7.0 using 0.1 mol L −1 NaOH or 0.1N HCl and the final volume was made up to 1 L. For preparation of solid media, 2% agar was added to the ...
Environmental Science and Pollution Research, Aug 4, 2021
The industrial revolution has marked a strong impact on financial upgradation of several countrie... more The industrial revolution has marked a strong impact on financial upgradation of several countries, and increase in the industrial establishment globally has direct impact on environment because of the release of unwanted product in air and inside the water bodies. The use of dyes has increased tremendously in various industries ranging from food, leather, textile, paper, cosmetic, pharmaceuticals, etc. The problem has emerged due to disposing of the dyes in the open environment, and mostly it is disposed along with the industrial wastes into the water bodies, which becomes harmful for animals, aquatic life and human health. This review highlights the role of the nanoparticles particularly biosynthesized nanoparticles for eliminating the dyes from the industrial wastewater. There are several methods for the synthesis of nanoparticle including physical, chemical and green synthesis of nanoparticles commonly known as biological method. Among all, the biological method is considered as the rapid, easy, eco-friendly and is being performed at mild conditions. The uses of nanoparticles for removal of dyes from water minimize the hazardous impact and thus considered to be the best approach as far as water quality and safety of environment is concerned.
Microbial Pathogenesis, Nov 1, 2019
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Life, Nov 13, 2021
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Annals of biotechnology, Feb 19, 2018
Alzheimer's disease is an unavoidable neurological disorder in which the death of brain cells cau... more Alzheimer's disease is an unavoidable neurological disorder in which the death of brain cells causes memory loss and cognitive decline and ultimate dementia. It is the most common cause of dementia in people of 65 years and older. It affects 10% of people over the age of 65 and 50% over the age of 85 years. Approximately 4million Alzheimer's patients in the United States (U.S.) and the annual treatment costs = $100 billion. It is the fourth leading cause of death in the United States and is becoming prevalent in many other countries. The total brain size shrinks with Alzheimer's-the tissue has progressively fewer nerve cells and connections. As such there is no known cure for Alzheimer's disease the death of brain cells in the dementia cannot be halted or reversed. Along with an aim to improve research in to prevention and treatment, the goals of the plan also include measures for present interventions. To help people suffering expand supports for people with Alzheimer's disease and their families, and enhance public awareness and engagement and expand your support towards them. Enhance care quality and efficiency. There are no disease-modifying drugs available for Alzheimer's disease but some options may reduce its symptoms and help improve quality of life and thereby help the patients to some extent. There are four drugs in a class called cholinesterase inhibitor approved for symptomatic relief in the US i.e., Donepezil (brand name Aricept), Alantamine (Reminyl), Rivastigmine and Tacrine (Cognex). A different kind of drug, memantine (Namenda), an N-methyl-D-aspartate (NMDA) receptor antagonist, may also be used, alone or in combination with a cholinesterase inhibitor. As with other types of dementia and neurodegenerative disease, a major part of therapy for patients with Alzheimer's comes from the support given by healthcare workers to provide dementia quality-of-life care, which becomes more important as needs increase with declining independence and increasing dependence.
Abstract book of the 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology" PLAMIC2020, 2020
Lipases are a multipurpose enzyme that holds a significant position in industrial applications du... more Lipases are a multipurpose enzyme that holds a significant position in industrial applications due to its ability to catalyse a large number of reactions such as hydrolysis, esterification, interesterification, transesterification which makes it a potential candidate. It is also used for the separation of chiral drugs from the racemic mixture and this property of lipase is considered very important in pharmaceutical industries for the synthesis of enantiopure bioactive molecules. Assuming the tremendous importance of lipases, as stereoselective biocatalysts, in pharmaceuticals and various other commercial applications, industrial enzymologists have been forced to search for those microorganisms which are able to produce novel biocatalysts at reasonably high yield. In the present study microbial lipase was isolated from the water sample of pond at Katra, Jammu and Kashmir (India). This enzyme has shown wide specificity and higher enantioselectivity, which make it pharmaceutical impor...
Journal of Materials Science, Sep 1, 2022
Sustainability
The present study focus on biosynthesis of stable silver nanoparticles (AgNPs) and zinc oxide nan... more The present study focus on biosynthesis of stable silver nanoparticles (AgNPs) and zinc oxide nanoparticles (ZnO NPs) from the leaf and stem extract of a therapeutic plant Carissa opaca. The visual observation, Fourier Transformed Infrared Spectroscopy (FTIR), Inductively Coupled Plasma analysis (ICP), High Resolution Transmission Electron Microscopy (HRTEM), and Scanning Electron Microscopy-Energy Dispersive Spectroscopy (SEM-EDS) were used to characterize and confirm the synthesized AgNPs and ZnO NPs. Afterwards; the synthesized nanoparticles were used to analyze their antimicrobial activity via in-vitro disk diffusion method against Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis, Aspergillus niger, and Candida albican. Both the nanoparticles showed maximum zone of inhibition against Pseudomonas aeruginosa (bacterial strain), whereas in the case of fungi, higher zone of inhibition was observed using ZnONPs against Candida albican and AgNPs against Aspergillus nig...
Environment Conservation Journal
Banganga is the first major station for a yatries when they undertake their on-foot journey from ... more Banganga is the first major station for a yatries when they undertake their on-foot journey from Katra to Bhawan (Shri Mata Vaishno Devi). The pilgrims reach a small bridge under which flows Banganga, the legendary river associated with the miracles and legends of Mata Vaishno Devi. There is water in the river all through the year. Various physico-chemical and biological parameters were taken into consideration during the course of study, these includes colour, temperature, DO, BOD, COD, pH, total hardness, total suspended solids and Total dissolved solids. Based on the results of this study it has concluded that slight increase of pollutants in downstream of the river was observed in comparison to the upstream, hence it is an alarming position of increasing pollution load in the river.
Future Microbiology
The microbial communities are an indispensable part of the human defense system and coexist with ... more The microbial communities are an indispensable part of the human defense system and coexist with humans as symbionts, contributing to the metabolic functions and immune defense against pathogens. An ecologically stable vaginal microbiota is dominated by Lactobacillus species, which plays an important role in the prevention of genital infections by controlling the vaginal pH, reducing glycogen to lactic acid, and stimulating bacteriocins and hydrogen peroxide. In contrast, an abnormal vaginal microbial composition is associated with an increased risk of bacterial vaginosis, trichomoniasis, sexually transmitted diseases, preterm labor and other birth defects. This microbial diversity is affected by race, ethnicity, pregnancy, hormonal changes, sexual activities, hygiene practices and other conditions. In the present review, we discuss the changes in the microbial community of the vaginal region at different stages of a female's life cycle and its influence on her reproductive heal...
Life, 2021
There is a unique microbial community in the female lower genital tract known as the vaginal micr... more There is a unique microbial community in the female lower genital tract known as the vaginal microbiota, which varies in composition and density and provides significant benefits during pregnancy, reproductive cyclicity, healthy newborn delivery, protection from preterm birth, infections such as UTIs, bacterial vaginosis, and so on, and improves the efficacy of treatments for vaginal cancers. Methods: It is necessary to know how the vaginal microbiome is composed in order to make an accurate diagnosis of the diseases listed above. A microbiome’s members are difficult to classify, and the way microbial communities function and influence host–pathogen interactions are difficult to understand. More and more metagenomic studies are able to unravel such complexities due to advances in high-throughput sequencing and bioinformatics. When it comes to vaginal microbiota research, we’ll be looking at the use of modern techniques and strategies that can be used to investigate variations in vag...