Inna Belenky - Academia.edu (original) (raw)

Papers by Inna Belenky

Research paper thumbnail of Fractional ablative and nonablative radiofrequency for skin resurfacing and rejuvenation of Thai patients

Journal of Cosmetic Dermatology, Aug 31, 2017

Background: Fractional radiofrequency (RF) technology is often the preferable skin resurfacing tr... more Background: Fractional radiofrequency (RF) technology is often the preferable skin resurfacing treatment, especially among Asian patients. Second generation fractional RF technology has exclusive capability to produce separate biological responses (ablation, coagulation, or a combination of both) with 3 distinguished penetration depth programs. Aims: The aim of this study was to evaluate the efficacy and safety of a fractional RF handpiece such as this, on the Thai population. Methods: Fifty-five Thai patients were treated with a fractional RF handpiece. The clinical assessment included a pain score, satisfaction survey, physician assessment, a combined patient and physician's assessment of skin condition, and clinical photographic assessments. The wound healing response was evaluated according to 5time points: immediately after applying a pulse, post 24 hours, post 7 days, post 1 month and post 8 weeks. Results: The obtained patient satisfaction score was "very satisfied" among 74% of the patients, post 3 sessions. Positive correlation was found between patient satisfaction and the physician's assessment. The skin condition assessment showed an increase from an average of 4.2 to 7.9. All treated symptoms improved after each treatment and the clinical outcome lasted at least up to 3-5 months. No significant adverse events were recorded. The in vivo prospective study showed a dose-related response in the deepness of the coagulation injury. In addition, there was evidence for a progressive healing process beginning shortly after exposure and completed within a week. Conclusions: This study clinically and histologically supports the efficacy of fractional RF handpiece in question with a high safety profile.

Research paper thumbnail of Intense Pulsed Light Pulse Configuration Manipulation Can Resolve the Classic Conflict Between Safety and Efficacy

PubMed, Nov 1, 2015

The widely used intense pulse light (IPL) technology was first commercially launched to the medic... more The widely used intense pulse light (IPL) technology was first commercially launched to the medical market in 1994 and similar to lasers, is based on the basic principle of selective photothermolysis. The main conflict during treatments with light-based technologies is between safety and efficacy of the treatment. The aim of this study was to evaluate new IPL technology, which integrates three different pulse configurations, with specific attention on the safety and efficacy of the treatment. 101 volunteers (with Fitzpatrick skin types I-VI) were treated as follows: 9 patients underwent 8 bi-weekly acne clearance treatments, 51 patients underwent 6-12 hair removal treatments, 11 patients were treated for general skin rejuvenation, 15 patients were treated for pigmentation lesions, and 15 patients were treated for vascular lesions. No serious adverse events were recorded. All patients that were treated for hair removal achieved significant hair clearance. The patients with facial rosacea responded the fastest to the treatment. Eight of nine patients that were treated for acne clearance achieved significant reduction in acne appearance. The results represented in this study support the approach that when taking into consideration both efficacy of the treatment and safety of the patients, the system should be "flexible" enough to allow exact treatment settings profile for each patient, according to their skin type and the symptom's biophysical characteristics. <

Research paper thumbnail of Multi-channeling optimized radiofrequency energy: A new age in well-established radiofrequency technology

World journal of dermatology, 2016

Multi-channeling optimized radiofrequency energy: A new age in well-established radiofrequency te... more Multi-channeling optimized radiofrequency energy: A new age in well-established radiofrequency technology

Research paper thumbnail of Local Tolerability and Performance Evaluation in Domestic Pigs of a Fractional Radiofrequency Device for Dermatologic Treatment

Toxicologic Pathology, May 20, 2020

Information on the safety of energy-based dermatological surgical devices in domestic pigs, and f... more Information on the safety of energy-based dermatological surgical devices in domestic pigs, and fractional radiofrequency (RF) devices in particular, is very limited. The aim of this study was to evaluate in a GLP-compliant study in domestic pigs the local reaction and performance of a novel fractional RF device. Five female domestic pigs were subjected to fractional RF pulses, using different energy and pulse durations and depth of penetration of the pulses. The animals were evaluated clinically and histologically at different time points (days 0, 1, 3, 7, and 14) postenergy exposure. There were no microscopic or macroscopic local adverse effects in any tested power settings, and there was time-related progressive healing, reaching complete macroscopic and microscopic healing by 7 days postapplication. As expected, there was power-related progressive increase in the incidence of ablation (destruction of skin tissue by vaporization) and coagulative necrosis of the dermis from low to high power setting. This comprehensive study, using multiple power settings (both ablative and coagulative) and several time points, will be of benefit for future studies evaluating new fractional RF devices.

Research paper thumbnail of Structure of the Regulatory Subunit of Acetohydroxyacid Synthase Isozyme III from Escherichia coli

Journal of Molecular Biology, 2006

The enzyme acetohydroxyacid synthase (AHAS) catalyses the first common step in the biosynthesis o... more The enzyme acetohydroxyacid synthase (AHAS) catalyses the first common step in the biosynthesis of the three branched-chain amino acids. Enzymes in the AHAS family generally consist of regulatory and catalytic subunits. Here, we describe the first crystal structure of an AHAS regulatory subunit, the ilvH polypeptide, determined at a resolution of 1.75 Å. IlvH is the regulatory subunit of one of three AHAS isozymes expressed in Escherichia coli, AHAS III. The protein is a dimer, with two babbab ferredoxin domains in each monomer. The two N-terminal domains assemble to form an ACT domain structure remarkably close to the one predicted by us on the basis of the regulatory domain of 3-phosphoglycerate dehydrogenase (3PGDH). The two C-terminal domains combine so that their b-sheets are roughly positioned back-toback and perpendicular to the extended b-sheet of the N-terminal ACT domain. On the basis of the properties of mutants and a comparison with 3PGDH, the effector (valine) binding sites can be located tentatively in two symmetrically related positions in the interface between a pair of N-terminal domains. The properties of mutants of the ilvH polypeptide outside the putative effector-binding site provide further insight into the functioning of the holoenzyme. The results of this study open avenues for further studies aimed at understanding the mechanism of regulation of AHAS by small-molecule effectors.

Research paper thumbnail of Local Tolerability and Performance Evaluation in Domestic Pigs of a Fractional Radiofrequency Device for Dermatologic Treatment

Toxicologic Pathology, 2020

Information on the safety of energy-based dermatological surgical devices in domestic pigs, and f... more Information on the safety of energy-based dermatological surgical devices in domestic pigs, and fractional radiofrequency (RF) devices in particular, is very limited. The aim of this study was to evaluate in a GLP-compliant study in domestic pigs the local reaction and performance of a novel fractional RF device. Five female domestic pigs were subjected to fractional RF pulses, using different energy and pulse durations and depth of penetration of the pulses. The animals were evaluated clinically and histologically at different time points (days 0, 1, 3, 7, and 14) postenergy exposure. There were no microscopic or macroscopic local adverse effects in any tested power settings, and there was time-related progressive healing, reaching complete macroscopic and microscopic healing by 7 days postapplication. As expected, there was power-related progressive increase in the incidence of ablation (destruction of skin tissue by vaporization) and coagulative necrosis of the dermis from low to...

Research paper thumbnail of Supplemental Material, sj-docx-1-tpx-10.1177_0192623320922958 - Local Tolerability and Performance Evaluation in Domestic Pigs of a Fractional Radiofrequency Device for Dermatologic Treatment

Supplemental Material, sj-docx-1-tpx-10.1177_0192623320922958 for Local Tolerability and Performa... more Supplemental Material, sj-docx-1-tpx-10.1177_0192623320922958 for Local Tolerability and Performance Evaluation in Domestic Pigs of a Fractional Radiofrequency Device for Dermatologic Treatment by Yuval Ramot, Guy Klaiman, Michal Steiner, Yossi Lavie, Inna Belenky and Abraham Nyska in Toxicologic Pathology

Research paper thumbnail of Fractional ablative and nonablative radiofrequency for skin resurfacing and rejuvenation of Thai patients

Journal of Cosmetic Dermatology, 2017

Background: Fractional radiofrequency (RF) technology is often the preferable skin resurfacing tr... more Background: Fractional radiofrequency (RF) technology is often the preferable skin resurfacing treatment, especially among Asian patients. Second generation fractional RF technology has exclusive capability to produce separate biological responses (ablation, coagulation, or a combination of both) with 3 distinguished penetration depth programs. Aims: The aim of this study was to evaluate the efficacy and safety of a fractional RF handpiece such as this, on the Thai population. Methods: Fifty-five Thai patients were treated with a fractional RF handpiece. The clinical assessment included a pain score, satisfaction survey, physician assessment, a combined patient and physician's assessment of skin condition, and clinical photographic assessments. The wound healing response was evaluated according to 5time points: immediately after applying a pulse, post 24 hours, post 7 days, post 1 month and post 8 weeks. Results: The obtained patient satisfaction score was "very satisfied" among 74% of the patients, post 3 sessions. Positive correlation was found between patient satisfaction and the physician's assessment. The skin condition assessment showed an increase from an average of 4.2 to 7.9. All treated symptoms improved after each treatment and the clinical outcome lasted at least up to 3-5 months. No significant adverse events were recorded. The in vivo prospective study showed a dose-related response in the deepness of the coagulation injury. In addition, there was evidence for a progressive healing process beginning shortly after exposure and completed within a week. Conclusions: This study clinically and histologically supports the efficacy of fractional RF handpiece in question with a high safety profile.

Research paper thumbnail of Origin of the specificities of acetohydroxyacid synthases and glyoxylate carboligase

Journal of Molecular Catalysis B: Enzymatic, 2009

... David M. Chipman a , Corresponding Author Contact Information , E-mail The Corresponding Auth... more ... David M. Chipman a , Corresponding Author Contact Information , E-mail The Corresponding Author , Ze'ev Barak a , Boaz Shaanan a , Maria Vyazmensky a , Elad Binshtein a , Inna Belenky a , Vered Temam a , Andrea Steinmetz b , 1 , Ralph Golbik b and Kai Tittmann b , 1. ...

Research paper thumbnail of Supplemental Material, Supp_Tables_06042020 - Local Tolerability and Performance Evaluation in Domestic Pigs of a Fractional Radiofrequency Device for Dermatologic Treatment

Supplemental Material, Supp_Tables_06042020 for Local Tolerability and Performance Evaluation in ... more Supplemental Material, Supp_Tables_06042020 for Local Tolerability and Performance Evaluation in Domestic Pigs of a Fractional Radiofrequency Device for Dermatologic Treatment by Yuval Ramot, Guy Klaiman, Michal Steiner, Yossi Lavie, Inna Belenky and Abraham Nyska in Toxicologic Pathology

Research paper thumbnail of Structure of the Regulatory Subunit of Acetohydroxyacid Synthase Isozyme III from Escherichia coli

Journal of Molecular Biology, 2006

The enzyme acetohydroxyacid synthase (AHAS) catalyses the first common step in the biosynthesis o... more The enzyme acetohydroxyacid synthase (AHAS) catalyses the first common step in the biosynthesis of the three branched-chain amino acids. Enzymes in the AHAS family generally consist of regulatory and catalytic subunits. Here, we describe the first crystal structure of an AHAS regulatory subunit, the ilvH polypeptide, determined at a resolution of 1.75 Å. IlvH is the regulatory subunit of one of three AHAS isozymes expressed in Escherichia coli, AHAS III. The protein is a dimer, with two babbab ferredoxin domains in each monomer. The two N-terminal domains assemble to form an ACT domain structure remarkably close to the one predicted by us on the basis of the regulatory domain of 3-phosphoglycerate dehydrogenase (3PGDH). The two C-terminal domains combine so that their b-sheets are roughly positioned back-toback and perpendicular to the extended b-sheet of the N-terminal ACT domain. On the basis of the properties of mutants and a comparison with 3PGDH, the effector (valine) binding sites can be located tentatively in two symmetrically related positions in the interface between a pair of N-terminal domains. The properties of mutants of the ilvH polypeptide outside the putative effector-binding site provide further insight into the functioning of the holoenzyme. The results of this study open avenues for further studies aimed at understanding the mechanism of regulation of AHAS by small-molecule effectors.

Research paper thumbnail of Multi-channeling optimized radiofrequency energy: A new age in well-established radiofrequency technology

Multi-channeling optimized radiofrequency energy: A new age in well-established radiofrequency te... more Multi-channeling optimized radiofrequency energy: A new age in well-established radiofrequency technology

Research paper thumbnail of Intense Pulsed Light Pulse Configuration Manipulation Can Resolve the Classic Conflict Between Safety and Efficacy

Journal of drugs in dermatology : JDD, 2015

The widely used intense pulse light (IPL) technology was first commercially launched to the medic... more The widely used intense pulse light (IPL) technology was first commercially launched to the medical market in 1994 and similar to lasers, is based on the basic principle of selective photothermolysis. The main conflict during treatments with light-based technologies is between safety and efficacy of the treatment. The aim of this study was to evaluate new IPL technology, which integrates three different pulse configurations, with specific attention on the safety and efficacy of the treatment. 101 volunteers (with Fitzpatrick skin types I-VI) were treated as follows: 9 patients underwent 8 bi-weekly acne clearance treatments, 51 patients underwent 6-12 hair removal treatments, 11 patients were treated for general skin rejuvenation, 15 patients were treated for pigmentation lesions, and 15 patients were treated for vascular lesions. No serious adverse events were recorded. All patients that were treated for hair removal achieved significant hair clearance. The patients with facial ros...

Research paper thumbnail of Pneumatic Delivery of Hyaluronan for Skin Remodeling: A Comparative Review

Journal of Drugs in Dermatology

Research paper thumbnail of Interactions between large and small subunits of different acetohydroxyacid synthase isozymes of Escherichia coli

Biochemistry, 2009

The large, catalytic subunits (LSUs; ilvB, ilvG and ilvI, respectively) of enterobacterial acetoh... more The large, catalytic subunits (LSUs; ilvB, ilvG and ilvI, respectively) of enterobacterial acetohydroxyacid synthases isozymes (AHAS I, II and III) have molecular weights 60 kDa and are paralogous with a family of other thiamin diphosphate dependent enzymes. The small, regulatory subunits ...

Research paper thumbnail of Acetohydroxyacid synthase isozyme I from Escherichia coli has unique catalytic and regulatory properties

Biochimica et Biophysica Acta (BBA) - General Subjects, 2006

AHAS I is an isozyme of acetohydroxyacid synthase which is apparently unique to enterobacteria. I... more AHAS I is an isozyme of acetohydroxyacid synthase which is apparently unique to enterobacteria. It has been known for over 20 years that it has many properties which are quite different from those of the other two enterobacterial AHASs isozymes, as well as from those of ''typical'' AHASs which are single enzymes in a given organism. These include a unique mechanism for regulation of expression and the absence of a preference for forming acetohydroxybutyrate. We have cloned the two subunits, ilvB and ilvN, of this Escherichia coli isoenzyme and examined the enzymatic properties of the purified holoenzyme and the enzyme reconstituted from purified subunits. Unlike other AHASs, AHAS I demonstrates cooperative feedback inhibition by valine, and the kinetics fit closely to an exclusive binding model. The formation of acetolactate by AHAS I is readily reversible and acetolactate can act as substrate for alternative AHAS I-catalyzed reactions. D

Research paper thumbnail of Clinical outcomes using Viora’s SVC™ technology: patients’ satisfaction, safety and comfort

Основное ограничение использования фракционных RF-систем первого поколения – невозможность создан... more Основное ограничение использования фракционных RF-систем первого поколения – невозможность создания коагуляции/некроза без абляции эпидермального слоя или наоборот – возможность абляции эпидермиса, но без коагуляции/некроза. Представлена технология Viora SVC™ второго поколения фракционных RF-систем. Тридцать добровольцев прошли курс из 3–6 процедур с применением фракционной RF-насадки аппарата V-touch. У 29 человек из 30 отмечалась заметная коррекция возрастных изменений, без осложнений и явных побочных эффектов. Благодаря широкому выбору параметров и уникальным свойствам, применение технологии SVC™ вызывает различные реакции в разных слоях кожи. Фракционная RF-манипула – RF-устройство нового поколения с расширенным контролем параметров, которое обеспечивает повышение эффективности и безопасности процедуры, а также устраняет дискомфорт во время ее проведения.

Research paper thumbnail of Intense Pulsed Light Pulse Configuration Manipulation Can Resolve the Classic Conflict Between Safety and Efficacy

Journal of drugs in dermatology : JDD, 2015

The widely used intense pulse light (IPL) technology was first commercially launched to the medic... more The widely used intense pulse light (IPL) technology was first commercially launched to the medical market in 1994 and similar to lasers, is based on the basic principle of selective photothermolysis. The main conflict during treatments with light-based technologies is between safety and efficacy of the treatment. The aim of this study was to evaluate new IPL technology, which integrates three different pulse configurations, with specific attention on the safety and efficacy of the treatment. 101 volunteers (with Fitzpatrick skin types I-VI) were treated as follows: 9 patients underwent 8 bi-weekly acne clearance treatments, 51 patients underwent 6-12 hair removal treatments, 11 patients were treated for general skin rejuvenation, 15 patients were treated for pigmentation lesions, and 15 patients were treated for vascular lesions. No serious adverse events were recorded. All patients that were treated for hair removal achieved significant hair clearance. The patients with facial ros...

Research paper thumbnail of Bi-Polar RF, Still the Gold Standard for Non-Invasive Fat Volume Reduction

Journal of Cosmetics, Dermatological Sciences and Applications, 2015

Research paper thumbnail of New vision in fractional radiofrequency technology with switching, vacuum and cooling

Journal of Cosmetic and Laser Therapy, 2014

Introduction: Since the introduction of fractional technology, various systems were launched to t... more Introduction: Since the introduction of fractional technology, various systems were launched to the market. The first generation of fractional RF systems created epidermal ablation with coagulative/necrosis of the dermis with sufficient clinical outcomes, but with some limitations. The aim of this study was to evaluate the efficacy and safety of SVC technology, based on the principle of separate biological responses. Material and methods: Fifty-two patients were treated for 3-6 sessions using fractional RF handpiece and eight patients received combination treatments with non-invasive RF handpiece. Results: All volunteers showed notable to significant improvement in the photoageing symptoms, without any significant complications or adverse events. Discussion: Due to its wide spectrum of parameters, the SVC technology can promote different biological responses. Owing to the &amp;amp;amp;amp;quot;Switching&amp;amp;amp;amp;quot; technology, the control of energy depth penetration enables delivery of the necessary thermal dose to the targeted skin layer. In addition, this novel technology includes the &amp;amp;amp;amp;quot;Vacuum&amp;amp;amp;amp;quot; and &amp;amp;amp;amp;quot;Cooling&amp;amp;amp;amp;quot; mechanisms, each contributing to the safety of the treatment. The Smart Heat function reduces the necessary energy levels and thereby reduces the pain level and risks for side effects.

Research paper thumbnail of Fractional ablative and nonablative radiofrequency for skin resurfacing and rejuvenation of Thai patients

Journal of Cosmetic Dermatology, Aug 31, 2017

Background: Fractional radiofrequency (RF) technology is often the preferable skin resurfacing tr... more Background: Fractional radiofrequency (RF) technology is often the preferable skin resurfacing treatment, especially among Asian patients. Second generation fractional RF technology has exclusive capability to produce separate biological responses (ablation, coagulation, or a combination of both) with 3 distinguished penetration depth programs. Aims: The aim of this study was to evaluate the efficacy and safety of a fractional RF handpiece such as this, on the Thai population. Methods: Fifty-five Thai patients were treated with a fractional RF handpiece. The clinical assessment included a pain score, satisfaction survey, physician assessment, a combined patient and physician's assessment of skin condition, and clinical photographic assessments. The wound healing response was evaluated according to 5time points: immediately after applying a pulse, post 24 hours, post 7 days, post 1 month and post 8 weeks. Results: The obtained patient satisfaction score was "very satisfied" among 74% of the patients, post 3 sessions. Positive correlation was found between patient satisfaction and the physician's assessment. The skin condition assessment showed an increase from an average of 4.2 to 7.9. All treated symptoms improved after each treatment and the clinical outcome lasted at least up to 3-5 months. No significant adverse events were recorded. The in vivo prospective study showed a dose-related response in the deepness of the coagulation injury. In addition, there was evidence for a progressive healing process beginning shortly after exposure and completed within a week. Conclusions: This study clinically and histologically supports the efficacy of fractional RF handpiece in question with a high safety profile.

Research paper thumbnail of Intense Pulsed Light Pulse Configuration Manipulation Can Resolve the Classic Conflict Between Safety and Efficacy

PubMed, Nov 1, 2015

The widely used intense pulse light (IPL) technology was first commercially launched to the medic... more The widely used intense pulse light (IPL) technology was first commercially launched to the medical market in 1994 and similar to lasers, is based on the basic principle of selective photothermolysis. The main conflict during treatments with light-based technologies is between safety and efficacy of the treatment. The aim of this study was to evaluate new IPL technology, which integrates three different pulse configurations, with specific attention on the safety and efficacy of the treatment. 101 volunteers (with Fitzpatrick skin types I-VI) were treated as follows: 9 patients underwent 8 bi-weekly acne clearance treatments, 51 patients underwent 6-12 hair removal treatments, 11 patients were treated for general skin rejuvenation, 15 patients were treated for pigmentation lesions, and 15 patients were treated for vascular lesions. No serious adverse events were recorded. All patients that were treated for hair removal achieved significant hair clearance. The patients with facial rosacea responded the fastest to the treatment. Eight of nine patients that were treated for acne clearance achieved significant reduction in acne appearance. The results represented in this study support the approach that when taking into consideration both efficacy of the treatment and safety of the patients, the system should be "flexible" enough to allow exact treatment settings profile for each patient, according to their skin type and the symptom's biophysical characteristics. <

Research paper thumbnail of Multi-channeling optimized radiofrequency energy: A new age in well-established radiofrequency technology

World journal of dermatology, 2016

Multi-channeling optimized radiofrequency energy: A new age in well-established radiofrequency te... more Multi-channeling optimized radiofrequency energy: A new age in well-established radiofrequency technology

Research paper thumbnail of Local Tolerability and Performance Evaluation in Domestic Pigs of a Fractional Radiofrequency Device for Dermatologic Treatment

Toxicologic Pathology, May 20, 2020

Information on the safety of energy-based dermatological surgical devices in domestic pigs, and f... more Information on the safety of energy-based dermatological surgical devices in domestic pigs, and fractional radiofrequency (RF) devices in particular, is very limited. The aim of this study was to evaluate in a GLP-compliant study in domestic pigs the local reaction and performance of a novel fractional RF device. Five female domestic pigs were subjected to fractional RF pulses, using different energy and pulse durations and depth of penetration of the pulses. The animals were evaluated clinically and histologically at different time points (days 0, 1, 3, 7, and 14) postenergy exposure. There were no microscopic or macroscopic local adverse effects in any tested power settings, and there was time-related progressive healing, reaching complete macroscopic and microscopic healing by 7 days postapplication. As expected, there was power-related progressive increase in the incidence of ablation (destruction of skin tissue by vaporization) and coagulative necrosis of the dermis from low to high power setting. This comprehensive study, using multiple power settings (both ablative and coagulative) and several time points, will be of benefit for future studies evaluating new fractional RF devices.

Research paper thumbnail of Structure of the Regulatory Subunit of Acetohydroxyacid Synthase Isozyme III from Escherichia coli

Journal of Molecular Biology, 2006

The enzyme acetohydroxyacid synthase (AHAS) catalyses the first common step in the biosynthesis o... more The enzyme acetohydroxyacid synthase (AHAS) catalyses the first common step in the biosynthesis of the three branched-chain amino acids. Enzymes in the AHAS family generally consist of regulatory and catalytic subunits. Here, we describe the first crystal structure of an AHAS regulatory subunit, the ilvH polypeptide, determined at a resolution of 1.75 Å. IlvH is the regulatory subunit of one of three AHAS isozymes expressed in Escherichia coli, AHAS III. The protein is a dimer, with two babbab ferredoxin domains in each monomer. The two N-terminal domains assemble to form an ACT domain structure remarkably close to the one predicted by us on the basis of the regulatory domain of 3-phosphoglycerate dehydrogenase (3PGDH). The two C-terminal domains combine so that their b-sheets are roughly positioned back-toback and perpendicular to the extended b-sheet of the N-terminal ACT domain. On the basis of the properties of mutants and a comparison with 3PGDH, the effector (valine) binding sites can be located tentatively in two symmetrically related positions in the interface between a pair of N-terminal domains. The properties of mutants of the ilvH polypeptide outside the putative effector-binding site provide further insight into the functioning of the holoenzyme. The results of this study open avenues for further studies aimed at understanding the mechanism of regulation of AHAS by small-molecule effectors.

Research paper thumbnail of Local Tolerability and Performance Evaluation in Domestic Pigs of a Fractional Radiofrequency Device for Dermatologic Treatment

Toxicologic Pathology, 2020

Information on the safety of energy-based dermatological surgical devices in domestic pigs, and f... more Information on the safety of energy-based dermatological surgical devices in domestic pigs, and fractional radiofrequency (RF) devices in particular, is very limited. The aim of this study was to evaluate in a GLP-compliant study in domestic pigs the local reaction and performance of a novel fractional RF device. Five female domestic pigs were subjected to fractional RF pulses, using different energy and pulse durations and depth of penetration of the pulses. The animals were evaluated clinically and histologically at different time points (days 0, 1, 3, 7, and 14) postenergy exposure. There were no microscopic or macroscopic local adverse effects in any tested power settings, and there was time-related progressive healing, reaching complete macroscopic and microscopic healing by 7 days postapplication. As expected, there was power-related progressive increase in the incidence of ablation (destruction of skin tissue by vaporization) and coagulative necrosis of the dermis from low to...

Research paper thumbnail of Supplemental Material, sj-docx-1-tpx-10.1177_0192623320922958 - Local Tolerability and Performance Evaluation in Domestic Pigs of a Fractional Radiofrequency Device for Dermatologic Treatment

Supplemental Material, sj-docx-1-tpx-10.1177_0192623320922958 for Local Tolerability and Performa... more Supplemental Material, sj-docx-1-tpx-10.1177_0192623320922958 for Local Tolerability and Performance Evaluation in Domestic Pigs of a Fractional Radiofrequency Device for Dermatologic Treatment by Yuval Ramot, Guy Klaiman, Michal Steiner, Yossi Lavie, Inna Belenky and Abraham Nyska in Toxicologic Pathology

Research paper thumbnail of Fractional ablative and nonablative radiofrequency for skin resurfacing and rejuvenation of Thai patients

Journal of Cosmetic Dermatology, 2017

Background: Fractional radiofrequency (RF) technology is often the preferable skin resurfacing tr... more Background: Fractional radiofrequency (RF) technology is often the preferable skin resurfacing treatment, especially among Asian patients. Second generation fractional RF technology has exclusive capability to produce separate biological responses (ablation, coagulation, or a combination of both) with 3 distinguished penetration depth programs. Aims: The aim of this study was to evaluate the efficacy and safety of a fractional RF handpiece such as this, on the Thai population. Methods: Fifty-five Thai patients were treated with a fractional RF handpiece. The clinical assessment included a pain score, satisfaction survey, physician assessment, a combined patient and physician's assessment of skin condition, and clinical photographic assessments. The wound healing response was evaluated according to 5time points: immediately after applying a pulse, post 24 hours, post 7 days, post 1 month and post 8 weeks. Results: The obtained patient satisfaction score was "very satisfied" among 74% of the patients, post 3 sessions. Positive correlation was found between patient satisfaction and the physician's assessment. The skin condition assessment showed an increase from an average of 4.2 to 7.9. All treated symptoms improved after each treatment and the clinical outcome lasted at least up to 3-5 months. No significant adverse events were recorded. The in vivo prospective study showed a dose-related response in the deepness of the coagulation injury. In addition, there was evidence for a progressive healing process beginning shortly after exposure and completed within a week. Conclusions: This study clinically and histologically supports the efficacy of fractional RF handpiece in question with a high safety profile.

Research paper thumbnail of Origin of the specificities of acetohydroxyacid synthases and glyoxylate carboligase

Journal of Molecular Catalysis B: Enzymatic, 2009

... David M. Chipman a , Corresponding Author Contact Information , E-mail The Corresponding Auth... more ... David M. Chipman a , Corresponding Author Contact Information , E-mail The Corresponding Author , Ze'ev Barak a , Boaz Shaanan a , Maria Vyazmensky a , Elad Binshtein a , Inna Belenky a , Vered Temam a , Andrea Steinmetz b , 1 , Ralph Golbik b and Kai Tittmann b , 1. ...

Research paper thumbnail of Supplemental Material, Supp_Tables_06042020 - Local Tolerability and Performance Evaluation in Domestic Pigs of a Fractional Radiofrequency Device for Dermatologic Treatment

Supplemental Material, Supp_Tables_06042020 for Local Tolerability and Performance Evaluation in ... more Supplemental Material, Supp_Tables_06042020 for Local Tolerability and Performance Evaluation in Domestic Pigs of a Fractional Radiofrequency Device for Dermatologic Treatment by Yuval Ramot, Guy Klaiman, Michal Steiner, Yossi Lavie, Inna Belenky and Abraham Nyska in Toxicologic Pathology

Research paper thumbnail of Structure of the Regulatory Subunit of Acetohydroxyacid Synthase Isozyme III from Escherichia coli

Journal of Molecular Biology, 2006

The enzyme acetohydroxyacid synthase (AHAS) catalyses the first common step in the biosynthesis o... more The enzyme acetohydroxyacid synthase (AHAS) catalyses the first common step in the biosynthesis of the three branched-chain amino acids. Enzymes in the AHAS family generally consist of regulatory and catalytic subunits. Here, we describe the first crystal structure of an AHAS regulatory subunit, the ilvH polypeptide, determined at a resolution of 1.75 Å. IlvH is the regulatory subunit of one of three AHAS isozymes expressed in Escherichia coli, AHAS III. The protein is a dimer, with two babbab ferredoxin domains in each monomer. The two N-terminal domains assemble to form an ACT domain structure remarkably close to the one predicted by us on the basis of the regulatory domain of 3-phosphoglycerate dehydrogenase (3PGDH). The two C-terminal domains combine so that their b-sheets are roughly positioned back-toback and perpendicular to the extended b-sheet of the N-terminal ACT domain. On the basis of the properties of mutants and a comparison with 3PGDH, the effector (valine) binding sites can be located tentatively in two symmetrically related positions in the interface between a pair of N-terminal domains. The properties of mutants of the ilvH polypeptide outside the putative effector-binding site provide further insight into the functioning of the holoenzyme. The results of this study open avenues for further studies aimed at understanding the mechanism of regulation of AHAS by small-molecule effectors.

Research paper thumbnail of Multi-channeling optimized radiofrequency energy: A new age in well-established radiofrequency technology

Multi-channeling optimized radiofrequency energy: A new age in well-established radiofrequency te... more Multi-channeling optimized radiofrequency energy: A new age in well-established radiofrequency technology

Research paper thumbnail of Intense Pulsed Light Pulse Configuration Manipulation Can Resolve the Classic Conflict Between Safety and Efficacy

Journal of drugs in dermatology : JDD, 2015

The widely used intense pulse light (IPL) technology was first commercially launched to the medic... more The widely used intense pulse light (IPL) technology was first commercially launched to the medical market in 1994 and similar to lasers, is based on the basic principle of selective photothermolysis. The main conflict during treatments with light-based technologies is between safety and efficacy of the treatment. The aim of this study was to evaluate new IPL technology, which integrates three different pulse configurations, with specific attention on the safety and efficacy of the treatment. 101 volunteers (with Fitzpatrick skin types I-VI) were treated as follows: 9 patients underwent 8 bi-weekly acne clearance treatments, 51 patients underwent 6-12 hair removal treatments, 11 patients were treated for general skin rejuvenation, 15 patients were treated for pigmentation lesions, and 15 patients were treated for vascular lesions. No serious adverse events were recorded. All patients that were treated for hair removal achieved significant hair clearance. The patients with facial ros...

Research paper thumbnail of Pneumatic Delivery of Hyaluronan for Skin Remodeling: A Comparative Review

Journal of Drugs in Dermatology

Research paper thumbnail of Interactions between large and small subunits of different acetohydroxyacid synthase isozymes of Escherichia coli

Biochemistry, 2009

The large, catalytic subunits (LSUs; ilvB, ilvG and ilvI, respectively) of enterobacterial acetoh... more The large, catalytic subunits (LSUs; ilvB, ilvG and ilvI, respectively) of enterobacterial acetohydroxyacid synthases isozymes (AHAS I, II and III) have molecular weights 60 kDa and are paralogous with a family of other thiamin diphosphate dependent enzymes. The small, regulatory subunits ...

Research paper thumbnail of Acetohydroxyacid synthase isozyme I from Escherichia coli has unique catalytic and regulatory properties

Biochimica et Biophysica Acta (BBA) - General Subjects, 2006

AHAS I is an isozyme of acetohydroxyacid synthase which is apparently unique to enterobacteria. I... more AHAS I is an isozyme of acetohydroxyacid synthase which is apparently unique to enterobacteria. It has been known for over 20 years that it has many properties which are quite different from those of the other two enterobacterial AHASs isozymes, as well as from those of ''typical'' AHASs which are single enzymes in a given organism. These include a unique mechanism for regulation of expression and the absence of a preference for forming acetohydroxybutyrate. We have cloned the two subunits, ilvB and ilvN, of this Escherichia coli isoenzyme and examined the enzymatic properties of the purified holoenzyme and the enzyme reconstituted from purified subunits. Unlike other AHASs, AHAS I demonstrates cooperative feedback inhibition by valine, and the kinetics fit closely to an exclusive binding model. The formation of acetolactate by AHAS I is readily reversible and acetolactate can act as substrate for alternative AHAS I-catalyzed reactions. D

Research paper thumbnail of Clinical outcomes using Viora’s SVC™ technology: patients’ satisfaction, safety and comfort

Основное ограничение использования фракционных RF-систем первого поколения – невозможность создан... more Основное ограничение использования фракционных RF-систем первого поколения – невозможность создания коагуляции/некроза без абляции эпидермального слоя или наоборот – возможность абляции эпидермиса, но без коагуляции/некроза. Представлена технология Viora SVC™ второго поколения фракционных RF-систем. Тридцать добровольцев прошли курс из 3–6 процедур с применением фракционной RF-насадки аппарата V-touch. У 29 человек из 30 отмечалась заметная коррекция возрастных изменений, без осложнений и явных побочных эффектов. Благодаря широкому выбору параметров и уникальным свойствам, применение технологии SVC™ вызывает различные реакции в разных слоях кожи. Фракционная RF-манипула – RF-устройство нового поколения с расширенным контролем параметров, которое обеспечивает повышение эффективности и безопасности процедуры, а также устраняет дискомфорт во время ее проведения.

Research paper thumbnail of Intense Pulsed Light Pulse Configuration Manipulation Can Resolve the Classic Conflict Between Safety and Efficacy

Journal of drugs in dermatology : JDD, 2015

The widely used intense pulse light (IPL) technology was first commercially launched to the medic... more The widely used intense pulse light (IPL) technology was first commercially launched to the medical market in 1994 and similar to lasers, is based on the basic principle of selective photothermolysis. The main conflict during treatments with light-based technologies is between safety and efficacy of the treatment. The aim of this study was to evaluate new IPL technology, which integrates three different pulse configurations, with specific attention on the safety and efficacy of the treatment. 101 volunteers (with Fitzpatrick skin types I-VI) were treated as follows: 9 patients underwent 8 bi-weekly acne clearance treatments, 51 patients underwent 6-12 hair removal treatments, 11 patients were treated for general skin rejuvenation, 15 patients were treated for pigmentation lesions, and 15 patients were treated for vascular lesions. No serious adverse events were recorded. All patients that were treated for hair removal achieved significant hair clearance. The patients with facial ros...

Research paper thumbnail of Bi-Polar RF, Still the Gold Standard for Non-Invasive Fat Volume Reduction

Journal of Cosmetics, Dermatological Sciences and Applications, 2015

Research paper thumbnail of New vision in fractional radiofrequency technology with switching, vacuum and cooling

Journal of Cosmetic and Laser Therapy, 2014

Introduction: Since the introduction of fractional technology, various systems were launched to t... more Introduction: Since the introduction of fractional technology, various systems were launched to the market. The first generation of fractional RF systems created epidermal ablation with coagulative/necrosis of the dermis with sufficient clinical outcomes, but with some limitations. The aim of this study was to evaluate the efficacy and safety of SVC technology, based on the principle of separate biological responses. Material and methods: Fifty-two patients were treated for 3-6 sessions using fractional RF handpiece and eight patients received combination treatments with non-invasive RF handpiece. Results: All volunteers showed notable to significant improvement in the photoageing symptoms, without any significant complications or adverse events. Discussion: Due to its wide spectrum of parameters, the SVC technology can promote different biological responses. Owing to the &amp;amp;amp;amp;quot;Switching&amp;amp;amp;amp;quot; technology, the control of energy depth penetration enables delivery of the necessary thermal dose to the targeted skin layer. In addition, this novel technology includes the &amp;amp;amp;amp;quot;Vacuum&amp;amp;amp;amp;quot; and &amp;amp;amp;amp;quot;Cooling&amp;amp;amp;amp;quot; mechanisms, each contributing to the safety of the treatment. The Smart Heat function reduces the necessary energy levels and thereby reduces the pain level and risks for side effects.