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Papers by Ioannis Chaniotakis

OAlib, 2024

The long-term relationship between humans and animals and the advantageous effects of animals on ... more The long-term relationship between humans and animals and the advantageous effects of animals on human health and well-being are well-documented in present literature. This relationship has evolved over the years, leading to a shift in human perception and behaviour towards animals. In our present day, Animal Assisted Intervention (AAI) programmes are regarded as contributors to human physical and psychological well-being. According to current literature, interactions with animals and AAI programmes appear to benefit children's psychological, emotional, social, and learning development. The article emphasises that while animals can contribute to children's development, this should not be considered a panacea but rather an additional means and part of a holistic intervention facilitated by accredited professionals.

The aim of this study was to evaluate the neuroendocrine and inflammation response to laparoscopi... more The aim of this study was to evaluate the neuroendocrine and inflammation response to laparoscopic total ovariohysterectomy in rabbits, by comparing surgical stress markers of laparoscopic group with those of conventional open ovariohysterectomy and open ovariohysterectomy with pre-incisional local anaesthesia groups. Blood was sampled from 18 rabbits, of which 6 underwent laparoscopic total ovariohysterectomy (TOH), 6 conventional open TOH and 6 conventional open TOH with pre-incisional local anaesthesia, 30 minutes before induction of anesthesia (T0), immediately after skin incision (T1), 90 minutes postoperatively (T2), and 24 hours postoperatively (T3). Cortisol and CRP serum, and ACTH, TNF-a, adrenaline, noradrenaline and IL-6 plasma concentrations were evaluated. Laparoscopic TOH in rabbits has advantages over the open surgical technique because it causes less surgical stress response in terms of serum cortisol concentrations immediately after skin incision (p=0.04) as well as...

EVSSAR – European Veterinary Society for Small Animal Reproduction, 2017

Although animal welfare has been of great concern for many years, an increased scientific interes... more Although animal welfare has been of great concern for many years, an increased scientific interest on prevention and reduction of animal suffering recently arose. Rabbits are very popular laboratory animals, farm animals and pets. Ovariohysterectomy (OHE) is a common surgical procedure in rabbits and is potentially painful unless appropriate analgesia is provided. Reduction of surgical stress and minimizing inflammatory response might significantly improve the welfare of laboratory animals [1]. Reduced pain associated with laparoscopic OHE has already been reported [2]. However, more rigorous comparisons of laparoscopic OHE versus so called open OHE by laparotomy have yet to establish a clear advantage [3]. The present study compared laparoscopic OHE with conventional open OHE and open OHE with pre-incisional local anaesthesia in rabbits. The aim was to indicate the most suitable approach for minimizing the surgical stress. Eighteen New Zealand white rabbits weighing 2.8\ub10.26 kg were divided into three groups of six animals each. Animals of group A underwent laparoscopic OHE, in rabbits of group B open OHE through a standardized 5-cm midline abdominal incision and in those of group C open OHE with a pre-incisional subcutaneous infusion of lidocaine 2% was performed. Blood samples were taken from each rabbit 30 minutes before induction of anesthesia (T0), after skin incision (T1), 90 minutes postoperatively (T2), and 24 hours postoperatively (T3). Cortisol and C-reactive protein (CRP) were measured in serum, and adrenocorticotropic hormone (ACTH), tumor necrosis factor-\u3b1 (TNF\u3b1), adrenaline, noradrenaline and interleukin-6 (IL-6) in plasma. Shapiro-Wilk, and t or Mann-Whitney U-tests were used for statistical analysis. Rabbits undergoing laparoscopic OHE demonstrated less serum cortisol response immediately after skin incision (T1) compared with rabbits of group B [t (6)=2,617, p=0.035)]. Adrenaline was significantly lower 24 h (T3) after laparoscopy than after open surgery [t (7)=2,610, p=0.035)]. At the same time point during open surgery, TNF-\u3b1 was significantly increased compared with laparoscopic surgery [t (5)=2,612, p=0.048)]. No significant difference was found for ACTH, noradrenaline, IL-6 and CRP levels between the groups. Our findings suggest that laparoscopic OHE causes less activation of the surgical stress system, as expressed by reduced secretion of cortisol and adrenaline. In addition, the laparoscopic technique seems to stimulate the inflammatory response to a lesser extent through reduced TNF-\u3b1 secretion, compared with the open technique with or without local anesthesia. These findings combined with faster recovery and less postoperative pain after minimally invasive OHE indicate that this technique may improve animal welfare in rabbits. [1] Flecknell PL. Refinement of animal use-assessment and alleviation of pain and distress. Lab Anim. 1994; 28:222-231. [2] Devitt CM, Cox RE, Hailey JJ. Duration, complications, stress, and pain of open ovariohysterectomy versus a simple method of laparoscopic-assisted ovariohysterectomy in dogs. J Am Vet Med Assoc. 2005;227:921-927. [3] Davidson EB, Moll HD, Payton ME. Comparison of laparoscopic ovariohysterectomy and ovariohysterectomy in dogs. Vet Surg 2004;33:62\u201369

Journal of Obstetrics and Gynaecology, 2018

Cancer Treatment and Research Communications

The Journal of surgical research, 2020

BACKGROUND Partial small bowel obstruction (SBO) is a common, potentially hazardous, surgical ent... more BACKGROUND Partial small bowel obstruction (SBO) is a common, potentially hazardous, surgical entity caused by numerous factors in humans. A number of techniques have been reported as efficient to simulate partial SBO in murine models. However, there is little data concerning their long-term survival. Our study presents a novel technique and evaluates its long-term efficiency compared with other commonly used techniques. MATERIALS AND METHODS Sixty C57BL/6 mice aged 6 to 8 wk were randomly divided into five intervention groups: ligation, intestinal ring, partial ligation, microclips, and the novel triple suture technique. The ring groups were subdivided into narrow, medium, and wide ring and partial ligation groups were subdivided at 1/3, 1/2, and 2/3 of the lumen. Survival cutoff time was set at 4 wk. Animals were then euthanized and small bowel muscle layer thickness was histopathologically evaluated. RESULTS None of the animals of the ligation and the ring groups reached the cuto...

Journal of the Hellenic Veterinary Medical Society, 2018

The mouse and the rat are currently overwhelmingly preferred as laboratory animals. Surgical rese... more The mouse and the rat are currently overwhelmingly preferred as laboratory animals. Surgical research on animals requires anesthesia and analgesia to obtain adequate immobility and to reduce stress and pain. Small rodent anesthesia is challenging for several reasons including the animals’ size, metabolic rate, high risk of hypothermia and difficulty in monitoring. The purpose of this study is to create an overview of the information in the anesthetic practices for small rodents, in particular mice and rats.

Society & Animals, 2018

The present study examined whether the perceptions/beliefs of the staff working with Military Dog... more The present study examined whether the perceptions/beliefs of the staff working with Military Dogs (MDs) may reveal possible factors affecting the welfare of MDs of the Hellenic Air Force Support Command (HAFSC). Visits were made to all Units (n= 5) that had MDs (n= 58) and military staff (n= 63) assigned to work with them. Each participant completed a questionnaire which included questions about demographic data and their perceptions and beliefs towards MDs. The research revealed evidence for possible factors that may affect the welfare of MDs. These factors were identified in the procedures of veterinary support, training, relaxation, and the general living conditions of MDs. According to the results, the recording of perceptions—beliefs of staff working with MDs—is a tool which can reveal possible factors affecting the dogs’ welfare. This study may stand as a guide for drafting welfare standards for MDs.

Pet Behaviour Science

There are organizations for re-homing animals that are also specialized in re-homing dogs and cat... more There are organizations for re-homing animals that are also specialized in re-homing dogs and cats who have been research subjects. Why can’t we do this for more animals and especially for the rats that are used extensively in research and they are not intended to be pets as they are not as common as pets? Even though previous research highlights neonatal handling as an essential process for rearing less stressed and emotionally sensitive adult rats, there is very little information about a specific neonatal handling schedule that could be a crucial factor in promoting their welfare and adoption as pets. The aim of this study is to propose a standardized handling schedule assessment based on implementation of a neonatal handling procedure that mimics maternal behavior, in order to have a positive effect on the rats’ welfare, decreasing their stress responsiveness towards humans and promoting their adoption as pets. DA rats (DA/OlaHsd) were handled during postnatal days 1 (D1) to 21 ...

BackgroundOsteosarcoma (OS) is the commonest primary osseous malignant tumor with a high propensi... more BackgroundOsteosarcoma (OS) is the commonest primary osseous malignant tumor with a high propensity to metastasize in lungs. Pulmonary widespread micrometastatic lesions are present in up to 80% of patients at initial diagnosis and they are associated with significantly worse prognosis. Doxycycline (Dox) is a synthetic tetracycline that has been shown to have anti-cancer properties in vitro and in vivo, and inhibit angiogenesis, effects that may prove beneficial for several types of cancer. The aim of the present work was to study how Dox affects OS cells’ growth in vitro and in vivo and OS-driven pulmonary metastasis in vivo. Methods In vitro, the effect of Dox was measured in MG-63 and 143B human OS cells’ viability, apoptosis, and migration. In vivo, highly metastatic143B cells were orthotopically implanted into the tibia of SCID mice and tumor growth as well as pulmonary metastases between Dox treated and untreated, non-amputated and early amputated xenografts were examined. Res...