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Papers by Iriana Moratto Carrara
Jornal Vascular Brasileiro
Background The MTHFR 677C>T variant’s involvement with hyperhomocysteinemia and peripheral art... more Background The MTHFR 677C>T variant’s involvement with hyperhomocysteinemia and peripheral arterial disease (PAD) is still unclear. Objectives To evaluate associations between the MTHFR 677C>T (rs1801133) variant and susceptibility to and severity of PAD and homocysteine (Hcy) levels. Methods The study enrolled 157 PAD patients and 113 unrelated controls. PAD severity and anatomoradiological categories were assessed using the Fontaine classification and the Inter-Society Consensus for the Management of Peripheral Arterial Disease (TASC), respectively. The variant was genotyped using real-time polymerase chain reaction and Hcy levels were determined using chemiluminescence microparticle assay. Results The sample of PAD patients comprised 60 (38.2%) females and 97 (61.8%) males. Patients were older and had higher Hcy than controls (median age of 69 vs. 45 years, p<0.001; and 13.66 µmol/L vs. 9.91 µmol/L, p=0.020, respectively). Hcy levels and the MTHFR 677C>T variant did n...
Journal of Photochemistry and Photobiology B: Biology, 2019
Cumulative ultraviolet (UV) exposure is associated with squamous skin cell carcinoma. UV radiatio... more Cumulative ultraviolet (UV) exposure is associated with squamous skin cell carcinoma. UV radiation induces oxidative modifications in biomolecules of the skin leading to photocarcinogenesis. Indeed, the cyclobutene pyrimidine dimers and other dimers formed by photoaddition between carbon-carbon bonds also have an important role in the initiation process. However, information on the systemic redox status during these processes is scarce. Thus, we investigated the systemic redox profile in UVB-induced squamous cell carcinoma in mice. Female hairless mice were exposed to UVB radiation (cumulative dose = 17.1 J/cm 2). The dorsal skin of these mice developed actinic keratosis (AK) and squamous cell carcinoma (SCC) and presented increased levels of oxidative and nitrosative stress biomarkers (4-hydroxy-2-nonenal and 3-nitrotyrosine), and decreased antioxidant defenses. Systemically, we observed the consumption of plasmatic antioxidant defenses and increased levels of advanced oxidized protein products (AOPP), an oxidative stress product derived from systemic inflammatory response. Taken together, our results indicate that UVB chronic irradiation leads not only to adjacent and tumoral oxidative stress in the skin, but it systemically is reflected through the blood. These new findings clarify some aspects of the pathogenesis of SCC and should assist in formulating better chemoprevention strategies, while avoiding additional primary SCC development and metastasis.
The aim of this study was to evaluate the association between the MTHFR 677C > T (rs1801133) g... more The aim of this study was to evaluate the association between the MTHFR 677C > T (rs1801133) genetic variant with susceptibility and severity of peripheral arterial occlusive disease (PAOD) and with serum levels of homocysteine (Hcy). This case-control study enrolled 157 patients with PAOD attended at University Hospital of Londrina, and unrelated 113 healthy individuals from Southern Brazil. The clinical severity of the PAOD patients was assessed by Fontaine classification and anatomoradiological categories by Inter-Society Consensus for the Management of Peripheral Arterial Disease (TASC). The MTHFR 677C > T was genotyped using real-time polymerase chain reaction. The PAOD patients showed higher Hcy than controls but the Hcy did not differ according to Fontaine and TASC categories. Patients carrying the TT genotype (recessive model) or CT + TT (dominant model) presented higher levels of Hcy than those carrying other genotypes. In conclusion, the T allele of MTHFR 677C > T...
Jornal Vascular Brasileiro
Background The MTHFR 677C>T variant’s involvement with hyperhomocysteinemia and peripheral art... more Background The MTHFR 677C>T variant’s involvement with hyperhomocysteinemia and peripheral arterial disease (PAD) is still unclear. Objectives To evaluate associations between the MTHFR 677C>T (rs1801133) variant and susceptibility to and severity of PAD and homocysteine (Hcy) levels. Methods The study enrolled 157 PAD patients and 113 unrelated controls. PAD severity and anatomoradiological categories were assessed using the Fontaine classification and the Inter-Society Consensus for the Management of Peripheral Arterial Disease (TASC), respectively. The variant was genotyped using real-time polymerase chain reaction and Hcy levels were determined using chemiluminescence microparticle assay. Results The sample of PAD patients comprised 60 (38.2%) females and 97 (61.8%) males. Patients were older and had higher Hcy than controls (median age of 69 vs. 45 years, p<0.001; and 13.66 µmol/L vs. 9.91 µmol/L, p=0.020, respectively). Hcy levels and the MTHFR 677C>T variant did n...
Journal of Photochemistry and Photobiology B: Biology, 2019
Cumulative ultraviolet (UV) exposure is associated with squamous skin cell carcinoma. UV radiatio... more Cumulative ultraviolet (UV) exposure is associated with squamous skin cell carcinoma. UV radiation induces oxidative modifications in biomolecules of the skin leading to photocarcinogenesis. Indeed, the cyclobutene pyrimidine dimers and other dimers formed by photoaddition between carbon-carbon bonds also have an important role in the initiation process. However, information on the systemic redox status during these processes is scarce. Thus, we investigated the systemic redox profile in UVB-induced squamous cell carcinoma in mice. Female hairless mice were exposed to UVB radiation (cumulative dose = 17.1 J/cm 2). The dorsal skin of these mice developed actinic keratosis (AK) and squamous cell carcinoma (SCC) and presented increased levels of oxidative and nitrosative stress biomarkers (4-hydroxy-2-nonenal and 3-nitrotyrosine), and decreased antioxidant defenses. Systemically, we observed the consumption of plasmatic antioxidant defenses and increased levels of advanced oxidized protein products (AOPP), an oxidative stress product derived from systemic inflammatory response. Taken together, our results indicate that UVB chronic irradiation leads not only to adjacent and tumoral oxidative stress in the skin, but it systemically is reflected through the blood. These new findings clarify some aspects of the pathogenesis of SCC and should assist in formulating better chemoprevention strategies, while avoiding additional primary SCC development and metastasis.
The aim of this study was to evaluate the association between the MTHFR 677C > T (rs1801133) g... more The aim of this study was to evaluate the association between the MTHFR 677C > T (rs1801133) genetic variant with susceptibility and severity of peripheral arterial occlusive disease (PAOD) and with serum levels of homocysteine (Hcy). This case-control study enrolled 157 patients with PAOD attended at University Hospital of Londrina, and unrelated 113 healthy individuals from Southern Brazil. The clinical severity of the PAOD patients was assessed by Fontaine classification and anatomoradiological categories by Inter-Society Consensus for the Management of Peripheral Arterial Disease (TASC). The MTHFR 677C > T was genotyped using real-time polymerase chain reaction. The PAOD patients showed higher Hcy than controls but the Hcy did not differ according to Fontaine and TASC categories. Patients carrying the TT genotype (recessive model) or CT + TT (dominant model) presented higher levels of Hcy than those carrying other genotypes. In conclusion, the T allele of MTHFR 677C > T...