Irina Korschineck - Academia.edu (original) (raw)

Papers by Irina Korschineck

Zenodo (CERN European Organization for Nuclear Research), Aug 29, 2023

PubMed, Jan 16, 1998

Myotonic dystrophy (DM) is the most common adult muscular dystrophy and follows an autosomal domi... more Myotonic dystrophy (DM) is the most common adult muscular dystrophy and follows an autosomal dominant pattern of inheritance. Up to now, the clinical diagnosis of DM was based on symptoms presented such as encephalopathy, facies myopathica, paresthesia, atrophy, myotonia, mental retardation, cataract, diabetes, cardiac conduction defects and electromyography. Since 1991 the specific molecular defect in DM is known and a respective diagnosis is possible. The mutation responsible for DM is the expansion of an unstable trinucleotide repeat, (CTG)n, in the 3'-untranslated region of the myotonin protein kinase gene. It is now generally accepted that the CTG repeat length correlates with the clinical category and the age at onset of the disease; therefore genetic tests are essential in monitoring and management of DM-patients and their family members. Based on the average incidence in Europe about 1000 affected individuals can be expected in Austria, a high percentage of whom is, however, not recognized as carries of the DM-mutation. After having established a genetic diagnosis in Austria allowing the detection of this mutation in DM-patients and their relatives, improvement of the diagnostic procedure should be possible.

Research Square (Research Square), Mar 22, 2021

Purpose: To evaluate the diagnostic accuracy of a commercially available test kit for noninvasive... more Purpose: To evaluate the diagnostic accuracy of a commercially available test kit for noninvasive prenatal determination of the fetal RhD status (NIPT-RhD) with a focus on early gestation and multiple pregnancies. Methods: The FetoGnost RhD assay (Ingenetix, Vienna, Austria) is routinely applied for clinical decision making either in woman with anti-D alloimmunization or in order to target the application of routine antenatal anti-D prophylaxis (RAADP) to women with a RhD positive fetus. Based on existing data in the laboratory information system the newborn's serological RhD status was compared with NIPT RhD results. Results: Since 2009 NIPT RhD was performed in 2,968 pregnant women between week 5+6 and 40+0 of gestation (median 12+6) and conclusive results were obtained in 2,888 (97.30%) cases. Diagnostic accuracy was calculated from those 2244 (77.70%) cases with the newborn's serological RhD status reported. The sensitivity of the FetoGnost RhD assay was 99.93% (95% CI 99.61%-99.99%) and the speci city was 99.61% (95% CI 98.86%-99.87%). No false positive or false negative NIPT RhD result was observed in 203 multiple pregnancies. Conclusion: NIPT RhD results are reliable when obtained with FetoGnost RhD assay. Targeted routine anti-D-prophylaxis can start as early as 11+0 weeks of gestation in singleton and multiple pregnancies.

European Journal of Cancer, 1993

Gene, Feb 1, 1997

ABSTRACT The gene encoding mouse protein C inhibitor (mPCI) was isolated and its nucleotide seque... more ABSTRACT The gene encoding mouse protein C inhibitor (mPCI) was isolated and its nucleotide sequence determined. Alignment of the genomic sequence with that of a cDNA obtained from mouse testis revealed that the mPCI gene (like the human counterpart) is composed of five exons and four introns with highly conserved exon/intron boundaries. It encodes a pre-polypeptide of 405 amino acids, which shows 63% identity with human PCI (hPCI). The putative reactive site is identical to that of hPCI from P5 to P3', suggesting a similar protease specificity. Also the putative heparin binding sites and 'hinge' regions are highly homologous in mouse and hPCI.

Gefäßwandumbau oder "vascular remodeling" ist definiert als Änderung im Gefäßkaliber, oft ohne Ve... more Gefäßwandumbau oder "vascular remodeling" ist definiert als Änderung im Gefäßkaliber, oft ohne Veränderung der Gesamtgewebsmasse. Die Verfügbarkeit des intravaskulären Ultraschalls hat dazu geführt, Gefäßremodeling als dynamischen Prozeß zu beobachten. Zelltod, Zellwachstum, Zellmigration und Produktion oder Abbau extrazellulärer Matrix sind durch die Expression von Genen in der Gefäßwand gesteuert. Anhand von Analysen solcher Genexpressionsmuster in verschiedenen Gefäßgebieten wird die Signifikanz des Forschungskonzepts "Genexpression in der Gefäßwand" an Beispielen illustriert. The importance of vascular remodeling defined as a change in calibre of the vessel, often with little or no change in overall tissue mass, has been increasingly recognized by intravascular ultrasound. Vascular remodeling results from cell death, cell growth, cell migration and matrix turnover as the consequence of a dynamic up-and downregulation of genes in the vascular wall. By defining expression patterns of known and novel genes in normal and diseased vessels, alterations of gene expression and potential targets for therapy are identified.

Veterinary Sciences

American foulbrood is caused by the spore-forming Paenibacillus larvae. Although the disease effe... more American foulbrood is caused by the spore-forming Paenibacillus larvae. Although the disease effects honey bee larvae, it threatens the entire colony. Clinical signs of the disease are seen at a very late stage of the disease and bee colonies are often beyond saving. Therefore, through active monitoring based on screening, an infection can be detected early and bee colonies can be protected with hygiene measures. As a result, the pressure to spread in an area remains low. The cultural and molecular biological detection of P. larvae is usually preceded by spore germination before detection. In this study, we compared the results of two methods, the culture detection and RT-PCR detection of DNA directly isolated from spores. Samples of honey and cells with honey surrounding the brood were used in a five-year voluntary monitoring program in a western part of Lower Austria. DNA-extraction from spores to speed up detection involved one chemical and two enzymatic steps before mechanical b...

Permissions: Requests for permissions to reproduce figures, tables, or portions of articles origi... more Permissions: Requests for permissions to reproduce figures, tables, or portions of articles originally published in Arteriosclerosis, Thrombosis, and Vascular Biology can be obtained via RightsLink, a service of the Copyright Clearance Center, not the Editorial Office. Once the online version of the published article for which permission is being requested is located, click Request Permissions in the middle column of the Web page under Services. Further information about this process is available in the Permissions and Rights Question and Answer document. Reprints: Information about reprints can be found online at:

Laboratory Investigation, 2003

Classic pulmonary thromboembolism research has documented that large pulmonary thromboemboli lyse... more Classic pulmonary thromboembolism research has documented that large pulmonary thromboemboli lyse spontaneously, suggesting potent fibrinolytic activity in human pulmonary artery (Pa). This concept conflicts with published animal studies in which the proximal Pa was reported to be devoid of tissue plasminogen activator (t-PA) expression. The current study used in situ hybridization protocols to demonstrate t-PA expression in samples of human main Pa (n ϭ 30). Real-time PCR was used to demonstrate quantitatively that the levels of t-PA transcripts were higher than those of its primary regulator [ie, plasminogen activator-inhibitor 1 (PAI-1)] in the Pa samples. Immunologic and functional assays extended these observations by demonstrating that levels of t-PA antigen were higher than PAI-1 antigen, which resulted in the detection of free t-PA activity. This contrasted with the fibrinolytic balance of matched samples of aorta (n ϭ 6) in which PAI-1 transcripts and antigen values were higher than the corresponding t-PA values, and only M r 110 kDa t-PA-PAI-1 complexes could be detected in functional assays. To assess the relative fibrinolytic contribution of the endothelial cell layer, Pa endothelial cells and aortic endothelial cells were scraped and propagated in culture for 20 Ϯ 6 days. Pa endothelial cell-conditioned media revealed increased t-PA/PAI-1 antigen ratios. Taken together, our data indicate that the balance between t-PA and PAI-1 is shifted in human main Pa to favor net PA activity.

Journal of Virological Methods, 1991

A procedure for sensitive detection of plum pox virus RNA in infected bark of trees is described.... more A procedure for sensitive detection of plum pox virus RNA in infected bark of trees is described. The method is based on the extraction of bark material with buffer containing proteinase K followed by partial purification of RNA using QUIAGEN anion exchange resin, The RNA is then reverse transcribed, the single stranded cDNA is amplified by the polymerase chain reaction using biot~yla~d deoxynucleotides as label. The amplified cDNA can subsequently be detected by spotting the reaction mixture onto a iritrocellulose membrane. After fixation and washing the incorporated label is detected enzymatically using streptavidinalkaline phosphatase. It was shown that this non-radioactive detection system is more sensitive than ELBA and a DNA/RNA hybridization test using 32P-labelled probes. It is also possible to detect plum pox virus infection with this assay in trees in the non-vege~tive period. Plum pox virus, PPV, Nicotiana clevelandii

Journal of Biological Chemistry, 2001

Forensic Science International, 2006

Conventional methods for the identification of different body fluids like blood, semen and saliva... more Conventional methods for the identification of different body fluids like blood, semen and saliva from biological stains involve immunological or enzymatic detection of certain proteins. In this study, we investigated potential RNA markers with the aim of developing Real-Time polymerase chain reaction (PCR) based methods to allow differentiation between several body fluids. Total RNA samples from artificially stained swabs and from various pieces of evidence from case work were extracted, amplified and analyzed with several RNA markers. Three assays detecting the body fluids of interest were selected: hemoglobin-alpha locus 1 (HBA), kallikrein 3 (KLK) and mucin 4 (MUC). With this approach, we demonstrate that specific Real-Time PCR assays are useful in identifying the source of the biological stain. Furthermore, RNA profiling of various body fluids was even possible on samples stored over a long period of time at ambient temperature. The stability and sensitivity of the applied method outlines a novel application for Real-Time PCR within the forensic field.

Arteriosclerosis, Thrombosis, and Vascular Biology, 1999

Archives of Gynecology and Obstetrics

Purpose To evaluate the diagnostic accuracy of a commercially available test kit for noninvasive ... more Purpose To evaluate the diagnostic accuracy of a commercially available test kit for noninvasive prenatal determination of the fetal RhD status (NIPT-RhD) with a focus on early gestation and multiple pregnancies. Methods The FetoGnost RhD assay (Ingenetix, Vienna, Austria) is routinely applied for clinical decision making either in woman with anti-D alloimmunization or to target the application of routine antenatal anti-D prophylaxis (RAADP) to women with a RhD positive fetus. Based on existing data in the laboratory information system the newborn’s serological RhD status was compared with NIPT RhD results. Results Since 2009 NIPT RhD was performed in 2968 pregnant women between weeks 5 + 6 and 40 + 0 of gestation (median 12 + 6) and conclusive results were obtained in 2888 (97.30%) cases. Diagnostic accuracy was calculated from those 2244 (77.70%) cases with the newborn’s serological RhD status reported. The sensitivity of the FetoGnost RhD assay was 99.93% (95% CI 99.61–99.99%) an...

BMC biotechnology, 2015

Celiac disease (CD) is a chronic, small intestinal inflammatory disease mediated by dietary glute... more Celiac disease (CD) is a chronic, small intestinal inflammatory disease mediated by dietary gluten and related prolamins. The only current therapeutic option is maintenance of a strict life-long gluten-free diet, which implies substantial burden for CD patients. Different treatment regimes might be feasible, including masking of toxic celiac peptides with blocking antibodies or fragments thereof. The objective of this study was therefore to select and produce a recombinant avian single-chain fragment variable (scFv) directed against peptic-tryptic digested gliadin (PT-Gliadin) and related celiac toxic entities. Gluten-free raised chicken of same age were immunized with PT-Gliadin. Chicken splenic lymphocytes, selected with antigen-coated magnetic beads, served as RNA source for the generation of cDNA. Chicken VH and VL genes were amplified from the cDNA by PCR to generate full-length scFv constructs consisting of VH and VL fragments joined by a linker sequence. ScFv constructs were ...

Environmental Pollution, 2015

Methods in Biotechnology, 1998

ABSTRACT The discovery that the expression of a viral coat protein in transgenic plants confers p... more ABSTRACT The discovery that the expression of a viral coat protein in transgenic plants confers protection to infection by homologous and related viruses (1–4) revolutionized the field of plant breeding. The approach of “coat protein-mediated protection” (CPMP) became an extensively studied strategy for many researchers and companies. Coat protein-mediated protection has been demonstrated to be effective against members of more than 10 groups of RNA viruses (3), but the molecular mechanism of the protection still remains unclear (5,6). The phenotype of virus-derived resistance in transformed plants can vary, case-by-case, from a simple delay in normal symptom development, or partial inhibition of virus replication, to complete immunity to challenge virus or viral RNA inoculation.

Journal of Medical Microbiology, 2014

The aminoglycoside phosphotransferase aph(3′)-IIa primarily inactivates kanamycin and neomycin, w... more The aminoglycoside phosphotransferase aph(3′)-IIa primarily inactivates kanamycin and neomycin, whilst aph(3′)-IIIa also inactivates amikacin. The aim of this study was to determine the frequency of both resistance genes in major human pathogens to obtain their baseline prevalence in the gene pool of these bacterial populations in Austria. In total, 10 541 Escherichia coli, Enterococcus faecalis, Enterococcus faecium, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica and Staphylococcus aureus isolates were collected representatively without selection bias between 2008 and 2011. Isolates were analysed by aph(3′)-IIIa/nptIII- and aph(3′)-IIa/nptII-specific TaqMan real-time PCR. For positive strains, MICs using Etests were performed and resistance gene sequences were determined. The overall prevalence of aph(3′)-IIIa/nptIII was 1.62 % (95 % confidence interval: 1.38–1.88 %). In Escherichia coli, enterococci, Staphylococcus aureus, P. aeruginosa and Salmonella spp., the aph(3′...

International Congress Series, 2006