Irina Maximova - Academia.edu (original) (raw)

Papers by Irina Maximova

Research journal of biotechnology/Research Journal of Biotechnology, Feb 29, 2024

Laser-induced forward transfer (LIFT) can be helpful for bioprinting of microbial cells, but data... more Laser-induced forward transfer (LIFT) can be helpful for bioprinting of microbial cells, but data on its impact on the microbial physiology are scarce. The aim of study was to investigate the LIFT effect on eukaryotic microorganisms (yeasts) growth characteristics. Candida albicans, Lipomyces lipofer and Saitozyma podzolica yeasts were printed on solid and liquid nutrient media and compared with traditional methods of inoculation. The laser bioprinting affects growth patterns of yeasts, leading to formation of subpopulations with different growth characteristics. The data obtained highlight necessity of studies of the LIFT effect on microorganisms' physiology.

Microbiome research reports, Feb 28, 2024

Studia Universitatis Moldaviae: Stiinte reale si ale naturii, 2015

Journal of the Science of Food and Agriculture, 2021

BACKGROUNDBlack soldier fly larvae (BSFL) offer very good prospects for the production of cheap a... more BACKGROUNDBlack soldier fly larvae (BSFL) offer very good prospects for the production of cheap and high‐quality dietary protein. This insect is able to consume low‐quality substrates, including food waste. The properties and safety of the compost obtained are largely determined by its microbiome. However, while the bacterial component of the BSFL‐compost microbiome has been much studied, little is known about its fungal component. In plant‐based rearing substrates both the biomass and the metabolic activity of fungi often exceed the biomass and the activity of prokaryotes. So, the purpose of this study was to investigate the fungal community of the compost produced by BSF larvae reared on a food waste substrate.RESULTSCommunity structure was determined by metabarcoding of the ITS (internal transcribed spacer) region. Species composition and abundance were determined using the plating technique and subsequent identification of the isolated pure cultures. It was found that the primar...

Biology Bulletin Reviews, 2019

Symbiotic microbes affect many aspects of the life of multicellular organisms and may favor their... more Symbiotic microbes affect many aspects of the life of multicellular organisms and may favor their adaptation to a changing environment, but there is little direct experimental evidence of such a contribution. To assess the possible role of the microbiome in the adaptation of Drosophila melanogaster to a high-NaCl feed substrate, we used two laboratory lines of salt-adapted flies (C1, C2) and two control lines cultivated on a standard feed without salt (H1, H2). We have already shown that the presowing of homogenate of flies C1 on the surface of a saline feed increases the breeding efficiency and enhances the development of drosophila larvae in comparison with a homogenate of H1 flies. We repeated this experiment for lines C2 and H2 and obtained similar data, which proves the reproducibility of the revealed effect. In addition, we found contrasting differences in the number and taxonomic composition of yeast in drosophila homogenates of the saltadapted and control lines. The results correspond to the assumption that changes in the symbiotic microbiome, including its yeast component, may contribute to the adaptation of drosophila to unfavorable feed substrates. The possible evolutionary consequences of such a contribution are discussed in this work.

Antonie van Leeuwenhoek, 2010

Studia Universitatis Moldaviae: Stiinte reale si ale naturii, 2019

Институт генетики, физиологии и защиты растений, Кишинев * МГУ им. М.В.Ломоносова, Москва DETERMI... more Институт генетики, физиологии и защиты растений, Кишинев * МГУ им. М.В.Ломоносова, Москва DETERMINAREA DIMENSIUNII OBIECTELOR MICROSCOPICE PRIN UTILIZAREA IMAGINILOR DIGITALE Metoda propusă este mai puţin dificilă, deoarece microscopul optic este necesar doar pentru obţinerea imaginii obiectelor măsurate, care se stabilește cu ajutorul obiect-micrometrului sau al reţelei camerei Goreaev. Utilizarea riglei de măsurare şi a imaginilor este mai comodă şi mai puţin dificilă în comparaţie cu cea obţinută cu ajutorul microscopului, micrometrului-ocular şi al mesei coordonative a microscopului. Modificarea propusă pentru analiza imaginilor digitale este comodă pentru cercetătorii care poartă ochelari, fiindcă diminuează încordarea aparatului vizual al cercetătorului. Cuvinte-cheie: dimensiuni ale obiectelor microscopice, imagini digitale. DETERMINATION OF MICROSCOPIC OBJECTS DIMENSIONS USING DIGITAL IMAGES The proposed method is less time consuming, since the microscope is needed only for obtaining the image of the measured object, as established of the object-micrometer or images of the Goryaev camera grid. Working with a scale ruler and an image is more comfortable and less difficult than working with a microscope, an ocular micrometer and a coordinate microscope table. Working with an image, unlike working with a microscope, is convenient for people using glasses as it reduces the burden on the researcher's vision.

Structure and Role of Microbial Communities in Southern Taiga Soils, 2000

General regularities in the structure of the microbial communities of southern taiga soil ecosyst... more General regularities in the structure of the microbial communities of southern taiga soil ecosystems and taxonomic differences between the microbial communities of soils with different hydrothermal characteristics are discussed with reference to the main types of soils of the Central State Forest Biosphere Reserve.

A New Yeast Species, Candida anutae sp. nov., from the Fruiting Bodies of Agarics, 2000

Among the yeasts isolated from the fruiting bodies of different species of agarics picked in fore... more Among the yeasts isolated from the fruiting bodies of different species of agarics picked in forests near Moscow and Turku (Finland) in 1995-1998, populations of an earlier unknown species, morphologically similar to Metschnikowia lunata but differing from it by physiological characteristics and the absence of asci with spores, were constantly found. Description of the new species is given within the genus Candida Berkhout.

Community Structure of Yeast Fungi in Forest Biogeocenoses, 2004

The results of longterm studies of yeasts inhabiting soil, plant surfaces, and plant residues in ... more The results of longterm studies of yeasts inhabiting soil, plant surfaces, and plant residues in typical subboreal forests of the European part of Russia are summarized. The cell number and species diversity of yeast communities in the array of substrates corresponding to succession stages in plant residue decomposition are shown to steadily decline. Each stage is characterized by its specific set of dominating species. The yeast diversity in forest biogeocenoses is shown to surpass that in other geographic zones. This manifests itself in a greater number of species occurring in similar arrays of substrates, in the absence of conspicuous dominants on the biogeocenotic level, and in a higher differentiation of the yeast population with respect to its habitat type. The forest yeast populations are also characterized by a high diversity of yeasts of ascomycetous affinity and of the anamorphic stages of Taphrinales and Tremellales and by the presence of typical pedobiont species (except Lipomyces spp.).

Destruction of soil aggregates in slope flows, 2007

Field studies and model experiments, as well as theoretical considerations, suggest that bed sedi... more Field studies and model experiments, as well as theoretical considerations, suggest that bed sediments represented by soil aggregates in overland flows on slopes exert a considerable influence on the intensity of erosion processes. In this context, one of the key problems in the development of adequate erosion models is the problem of the rate of destruction of such aggregates in the flows. The results of experimental studies of the destruction of aggregates of chernozemic soils are analyzed. It is found that the destruction of soil aggregates in the flow proceeds in two stages. During the first stage, the aggregates are rapidly broken apart into smaller fragments. During the second stage, these fragments are subjected to abrasion. An equation describing the destruction of aggregates upon their movement in the flow in dependence on the aggregate size and the distance of aggregate transport is suggested. The effect of some groups of soil microorganisms on the aggregate resistance to the destruction is shown.

Pigmented Basidiomycetous Yeasts Are a Promising Source of Carotenoids and Ubiquinone Q 10, 2008

Strains of basidiomycetous yeasts isolated from different sources were studied in order to determ... more Strains of basidiomycetous yeasts isolated from different sources were studied in order to determine the content of carotenoid pigments and ubiquinone Q 10 for subsequent selection work to obtain producers of these substances. The high specific productivity of carotenoids (600-700 mg/g) was revealed in the representatives of the following species: Cystofilobasidium capitatum, Rhodosporidium diobovatum, R. sphaerocarpum, Rhodotorula glutinis, Rh. minuta , and Sporobolomyces roseus. The ratio of the major pigments (torulene, torularhodine, and β-carotene) in the representatives of different species was studied. Certain specific features of pigment formation in relation to the taxonomic position of the yeasts were determined. Eurybiont species with substantial ecological lability are the most active producers of carotenoids and ubiquinone Q 10 among the epiphytes. It is the first time a comparative analysis of the coenzyme Q 10 content in different taxa has been performed using several strains of the same species. The maximal coenzyme Q 10 production (1.84 mg/g of dry biomass) was found in the yeast species R. sphaerocarpum.

Spatial Structure of Epiphytic Yeast Communities on Fruits of Sorbus aucuparia L., 2009

The subject of this research is epiphytic yeast communities formed on the surface of Sorbus aucup... more The subject of this research is epiphytic yeast communities formed on the surface of Sorbus aucuparia. The object is to make quantitative assessment of the yeast communities' differentiation of the same but distant substratum. Results of the nested ANOVA demonstrated that with increase in distances, there are increases in the variation of total number and relative abundance of the dominant yeast communities. The average similarity between groups of single fruits (Sørensen's f Similarity Coefficient) regularly decreased with distance. The results demonstrate that the number and structure of separate yeast groups depend not only on ecological factors but also on proximity to other communities. Such aggregation in the distribution of the microorganisms' species caused by migration and colonial resettlement should be taken into account when analyzing their diversity in natural habitats.

Ogataea cecidiorum sp. nov., a methanol-assimilating yeast isolated from galls on willow leaves, 2010

Ten strains of a new endophytic ascosporeforming, methanol-assimilating yeast were isolated from ... more Ten strains of a new endophytic ascosporeforming, methanol-assimilating yeast were isolated from the galls induced by sawflies on the leaves of willows in the Losiny Ostrov National Park (Moscow region). Standard phenotypical tests and phylogenetic analyses of 18S rRNA gene, 5.8S-ITS gene region and 26S rRNA gene (D1/D2 domains) sequences showed that the species belongs to the genus Ogataea. We describe it as Ogataea cecidiorum and designate type culture KBP Y-3846 (= CBS 11522 T = VKM Y-2982 T = VKPM Y-3482 T = MUCL 52544 T = NCAIM Y.01965 T) as the type strain. The new species was registered in MycoBank under MB 515233.

Yeast Communities of Formica aquilonia Colonies, 2016

Yeast abundance and species diversity in the colonies of Formica aquilonia ants in birch-pine gra... more Yeast abundance and species diversity in the colonies of Formica aquilonia ants in birch-pine grass forest near Novosibirsk, Russia, were studied. The average yeast number in the anthill material was 10 3-10 4 CFU/g, reaching 10 5 CFU/g in the hatching chambers. Typical litter species (Trichosporon moniliiforme and Cystofilobasidium capitatum) were predominant in soil and litter around the anthills. Apart from these species, ascomycete species of the family Debaryomycetaceae, Debaryomyces hansenii, and Schwanniomyces vanrijiae were predominant in the anthill material. Yeast population of the ant' bodies consisted exclusively of the members of the last two species. Thus, highly specific yeast communities formed in the colonies of Formica aquilonia ants differ from the communities of surrounding soil. These differences are caused by environment-forming activity of the ants.

Yeasts in Hevea brasiliensis Latex, 2016

Yeast abundance and species diversity in the latex of rubber tree Hevea brasiliensis (Willd. ex J... more Yeast abundance and species diversity in the latex of rubber tree Hevea brasiliensis (Willd. ex Juss.) Müll. Arg., on its green leaves, and in soil below the plant were studied. The yeasts present in the fresh latex in numbers of up to 5.5 log(CFU/g) were almost exclusively represented by the species Candida heveicola. This species was previously isolated from Hevea latex in China. In the course of natural modification of the latex (turned from liquid to solid form), yeast diversity increased, while yeast abundance decreased. The yeasts in thickened and solidified latex were represented by typical epiphytic and ubiquitous species: Kodamea ohmeri, Debaryomyces hansenii, Rhodotorula mucilaginosa, and synanthropic species Candida parapsilosis and Cutaneotrichosporon arboriformis. The role of yeasts in latex modification at the initial stages of succession and their probable role in development of antifungal activity in the latex are discussed.

Experimental Study of Factors Affecting Soil Erodibility, 2018

⎯The effect of different factors and preparation conditions of monofraction samples from the arab... more ⎯The effect of different factors and preparation conditions of monofraction samples from the arable horizon of leached chernozem on soil erodibility and its relationship with soil tensile strength (STS) has been studied. The exposure of samples at 38°C reduces their erodibility by two orders of magnitude. The drying of samples, on the contrary, increases their erodibility. It has been shown that erodibility decreases during the experiment. It has been found that the inoculation of soil with yeast cultures (Naganishia albida, Lipomyces tetrasporus) reliably increases the STS value in 1.5-1.9 times. The sterile soil is eroded more intensively than the unsterile soil: at 4.9 and 0.3 g/(m 2 s), respectively. The drying of soil followed by wetting to the initial water content (30%) has no significant effect on the STS value in almost all experimental treatments.

Soil Microbial Communities of Eastern Antarctica, 2018

Investigation of microbial communities of Antarctica soils is a very important field of research ... more Investigation of microbial communities of Antarctica soils is a very important field of research that expands our knowledge of microbial participation in primary soil formation and specific features of their communities in extreme habitats, and it is of considerable interest in directed search of for microorganisms as potential biotechnological objects. The results of long-term (2012-2017) complex studies on soil microbial communities of the Russian East Antarctica polar stations at Shirmakher oasis (Novolazarevskaya station), the Larsemann Hills (Progress station), and the Tala Hills (Molodezhnaya station) are presented in this review. The assessment of biomass of soil microorganisms by the methods of direct microscopy has been carried out for the first time for this region. The general amount of microbial biomass is small; the fungi dominate (77-99%). The unique features of Antarctic soils are the high content and morphological diversity of small forms of microorganisms: fungi are presented by mainly single-celled structures (small spores and yeasts), while bacteria by ultrafine (filtering) forms. At the same time, microorganisms can significantly contribute to such important ecological functions of soil as the emission of greenhouse gases, especially during the warm season with the stable positive temperatures of the soil. This should be considered during creation of models and forecasts of global warming. The use of various isolation techniques for the analysis of the soil microbial population, together with the succession approach, significantly expand the information about taxonomic diversity of cultivated fungi and bacteria in Antarctica soils.

Yeast Communities Associated with Diptera of the White Sea Littoral, 2020

Relationship between symbiotic yeast and Diptera insects are studied mainly on fruit flies (Droso... more Relationship between symbiotic yeast and Diptera insects are studied mainly on fruit flies (Drosophilidae). Evolutionary experiments showed that changes in the composition of the yeast microbiota vectored by flies in their gut and on the body surface contributes to the adaptation of laboratory Drosophila populations to the high-salt growth medium. Although saline substrates are not commonly used by Drosophilidae in nature, species adapted to such substrates are known in other families of Diptera. Yeast communities associated with these species are studied insufficiently. This is the first report on the yeast communities associated with two Diptera species living near the White Sea, Paracoenia fumosa (Stenhammar, 1844) (Ephydridae) and Fucellia fucorum (Fallén, 1819) (Anthomyiidae). Their larvae develop in the coastal saline lagoons and in algae driven ashore. The P. fumosa yeast microbiota includes opportunistic species Pichia kluyveri Bedford ex Kudryavtsev, 1960 and P. kudriavzevii Boidin, Pignal, Besson, 1965 and is characterized by its highly variable abundance (the number of colony-forming units varies widely in different individuals). F. fucorum is characterized by a more stable yeast community, including species common in White Sea algae and coastal waters (Debaryomyces hansenii (Zopf) Lodder, Kreger-van Rij, 1952, Metschnikowia zobellii (van Uden, Castelo-Branco) van Uden, 1962, and M. bicuspidata (Metschnikoff) Kamienski, 1899). These two species of littoral flies shared no common yeast species. Moreover, none of the yeast species found in the littoral flies was detected in the evolutionary experiment on the adaptation of Drosophila to the high-salt growth medium (the similarity is limited only to the Pichia genus level). Contrasting differences in the yeast microbiome of two ecologically similar littoral species of Diptera may indicate, on the one hand, specificity, and, on the other, evolutionary plasticity of the relationship between salt-tolerant flies and yeast.

Yamadazyma cocois f.a., sp. nov., an ascomycetous yeast isolated from coconuts, 2020

The GenBank/EMBL/DDBJ accession numbers for 18S (SSU), ITS region and D1/D2 domains of the LSU rR... more The GenBank/EMBL/DDBJ accession numbers for 18S (SSU), ITS region and D1/D2 domains of the LSU rRNA gene, and for TEF1 and RPB1 genes of type strain VCIM 4241 are MN764367, MN764369, LR740761, and LR740762, respectively. Other sequences obtained in this study are listed in Table 1S. The Mycobank number is MB 834435. A supplementary table is available with the online version of this article.