Irineo Torres Pacheco - Profile on Academia.edu (original) (raw)

Papers by Irineo Torres Pacheco

The Príncipe Gold Deposit arouses economic interest since the gold cycle in 1724. There are no re... more The Príncipe Gold Deposit arouses economic interest since the gold cycle in 1724. There are no reports of publications regarding to near-surface geophysics studies in the study area; hence, this paper reports the use of geoelectrical data for subsurface models. Furthermore, this research has as objective the identification of mineralized zones. The Príncipe Gold Deposit is orogenic and it is known as a pressure shadow zone that acted as structural traps for the ore . Then, two profiles with resistivity and IP data for two lines were conducted perpendicularly to the main structural trend N10ºW-N20ºE, with 10 meters spacing between electrodes. Granite with intense deformation was interpreted as more resistive zones (1000-4000 ohm.m), shear zones with approximately 670 ohm.m and mineralized zones were interpreted as greater than 14 mV/V values.

Methylation profile and phenotypical changes in Capsicum annum L. under water deficit and H2O2 application

2017 XIII International Engineering Congress (CONIIN), 2017

In recent years has been suggested that foliar application of H2O2 alleviate symptoms of drought ... more In recent years has been suggested that foliar application of H2O2 alleviate symptoms of drought in several crops. The aim of this work was to assess the effect of exogenous H2O2 application on Capsicum annuum L. crop as a protective treatment to further water deprivation. DNA methylation (5-methylcytosine percentage), stomatal density, number of leaves and photosynthetic activity were the variables evaluated.

Acta Horticulturae, 2016

miRNAs are a class of small non-coding RNAs that regulate gene expression in eukaryotes. They are... more miRNAs are a class of small non-coding RNAs that regulate gene expression in eukaryotes. They are involved in the control of many plant developmental processes, including fruit development. The information available on miRNAs, related to expression, abundance, and conservation in several species, provides a new opportunity to study the role of miRNAs in non-model species, such as Opuntia ficus indica. In this work, bioinformatics and molecular biology methods were combined to isolate, identify and select candidate miRNAs in order to analyze their expression profiles during prickly pear development. The profiling comparison detected the expression of different miRNAs during prickly pear development, which were clustered in different expression patterns. A gradual increase in the expression of several miRNAs was observed during fruit development. The results described in this work, represent the first report of miRNA expression profiles during prickly pear fruit development; and provide the basis for future research on miRNAs on Opuntia.

Presencia De Compuestos Relacionados Con La Salud en Dos Especies De Chile

octi.guanajuato.gob.mx

El consumo de chile está ligado a la historia de América y en particular a la de México. Colón de... more El consumo de chile está ligado a la historia de América y en particular a la de México. Colón descubrió que en este continente no existía la pimienta pero encontró otras especies de plantas con propiedades interesantes, entre las que destacaba el chile al que bautizó con el ...

Agriculture, 2020

Viruses are estimated to be responsible for approximately 50% of the emerging plant diseases, whi... more Viruses are estimated to be responsible for approximately 50% of the emerging plant diseases, which are difficult to control, and in some cases, there is no cure. It is essential to develop therapy practices to strengthen the management of these diseases caused by viruses in economically important crops. Metal nanoparticles (MeNPs) possess diverse physicochemical properties that allow for them to have a wide range of applications in industry, including nanomedicine and nano-agriculture. Currently, there are reports of favorable effects of the use of nanoparticles, such as antibacterial, antifungal, and antiviral effects, in animals and plants. The potential antiviral property of MeNPs makes them a powerful option for controlling these histological agents. It is crucial to determine the dosage of NPs, the application intervals, their effect as a biostimulant, and the clarification of the mechanisms of action, which are not fully understood. Therefore, this review focuses on discussin...

Influence of Salicylic Acid application on Oxidative and Molecular Responses and functional properties of Capsicum annuum L. cultivated in greenhouse conditions

Proceedings of The 1st International Electronic Conference on Metabolomics, 2016

Modifications in growing techniques can affect the yield and nutritional quality of various culti... more Modifications in growing techniques can affect the yield and nutritional quality of various cultivated plant species. Owing to its high nutritional value, sweet pepper (Capsicum annuum L.) was used in this study as a model plant to investigate the effect of abiotic stress (Electrical conductivity [EC]) and elicitor (Salicylic acid [SA]) on yield and fruit quality parameters under conditions of greenhouse. Nonetheless, the application of elicitors, are stable molecules that induce the activation of transduction cascades and hormonal pathways, which trigger induced resistance to environmental stress. The combination in metabolomics of advanced analytical methods and bioinformatics tools provides wide chemical compositional data that contributes to corroborate (or not) the substantial equivalence. The aim was evaluated the endogenous H2O2 production caused by the effect of different concentrations of (SA) in Capsicum annuum L. in production after elicitation in seedling, and the production of secondary metabolites using GC-MS (flavonoids, tannins and phenolic content as well as the antioxidant properties) in plant and fruit of Capsicum annuum L. to relate their response in metabolic pathways. Results displayed that the use SA, induced an endogenous H2O2 and enzymatic activities related with plant defense as phenylalanine ammonia lyase and catalase. This production showed results that could be an indicator for determining application opportunity uses in agriculture for maintaining plant alert systems against a stress. These results were correlated with those obtained from cultivation of capsicum annum until obtaining the fruit at ripe stage had a high content of bioactive compounds that exhibited significant antioxidant properties. The correlation of the contents of flavonoids, tannins and total phenolics and the ability to remove free radicals of the sweet pepper was significant (r = 0.99, P <0.000) in treatment 3 (0.1 mM SA/36 dS/m EC) showed a correlation between the DPPH and ABTS compared with the control. The analysis revealed that significant increased activity of CAT and PAL and H2O2 endogenous were significantly (p < 0.05) perturbed in the stress-induced treatments: in some metabolic pathways as aminoacyl t-RNA biosynthesis.

International Journal of Molecular Sciences (Elicitors-2013)

Strategies for Sustainable Plant Food Production: Facing the Current Agricultural Challenges—Agriculture for Today and Tomorrow

Biosystems Engineering: Biofactories for Food Production in the Century XXI, 2014

ABSTRACT Plants are fundamental elements of the human diet, either as direct sources of nutrients... more ABSTRACT Plants are fundamental elements of the human diet, either as direct sources of nutrients or indirectly as feed for animals. During the past years, the main goal of agriculture has been to increase yield in order to provide the food that is needed by a growing world population. However, as important as yield, but commonly forgotten in conventional agriculture, is to retain and, if possible, to increase the phytochemical content due to their health implications. By 2025, the global population will exceed 7 billion. In the short term, per capita availability of arable land and irrigation water will decrease from year to year while biotic and abiotic stresses expand. Food security, defined as economic, physical, and social access to a balanced diet and harmless drinking water will be a compromise, with a holistic approach to nutritional and non-nutritional factors needed to achieve success in the eradication of hunger. Science and technology will play a very important role in stimulating and sustaining agriculture leading to long-term increases in productivity without linked ecological harm.

Brain Sciences, 2021

This research assesses the brain activity and visual performance at baseline and after light ther... more This research assesses the brain activity and visual performance at baseline and after light therapy (LTH), of seventeen patients with strabismus and amblyopia (SA), and eleven healthy controls (HCs) from Querétaro, México. Quantitative electroencephalogram analysis (qEEG) was used to record the brain activity, and clinical metrics such as the visual acuity, angle of deviation, phoria state, stereopsis, and visual fields determined the visual performance. Results showed a constant higher alpha-wave frequency for HCs. Low voltages remained negative for HCs and positive for SA patients across stimulation. After LTH, high voltage increased in SA patients, and decreased in HCs. A second spectral peak, (theta-wave), was exclusively recorded in SA patients, at baseline and after LTH. Positive Spearman correlations for alpha-wave frequency, low and high voltages were only seen in SA patients. Synchronized brain activity was recorded in all SA patients stimulated with filters transmitting l...

IEEE Access, 2021

This research measures and compares the cortical activity at baseline and during light stimulatio... more This research measures and compares the cortical activity at baseline and during light stimulation (LS) in patients with strabismus and amblyopia (SA) and healthy controls (HCs), to understand the differences in its functionality and propose LS as a potential brain stimulator. This observational, longitudinal, prospective study enrolled 17 SA patients and 17 HCs from Querétaro, México. Electroencephalography (EEG) and digital brain mapping (DBM) were used to identify changes in frequency, voltage, and brain coherence. A total of 68 DBM was analyzed for this purpose. Our results indicate that at baseline, patients with strabismus and amblyopia present: i) lower frequency of alpha-wave activity (p = 0.029) with an abnormal distribution within hemispheres, ii) theta-wave with a predominance in the frontal lobes, which relates these visual conditions to neurodevelopmental disorders, iii) higher values of low voltage (p < 0.001) and lower values of and high voltage (p = 0.001) iv) interhemispheric asynchronicity with a predominance in the left hemisphere. On the other hand, the administration of LS modulates the brain activity of SA patients by i) modifying high and low voltages (p < 0.001 and 0.022 respectively), which define the anteroposterior gradient ii) eliminating theta-waves, iii) distributing alpha-wave activity towards the occipital lobes iv) bringing synchronicity between hemispheres. For the HC group, LS alters the distribution of alpha waves within hemispheres, and the state of interhemispheric synchronicity. There were no statistically significant changes in the frequency of the alpha-wave or the anteroposterior gradient. To summarize, LS provokes a state of malleability in the brain of SA patients, by increasing the cortical connectivity, enhancing neural activation and bringing to balance the interhemispheric communication, which converts it into a potential brain stimulator that should be considered as a complementary therapy in the treatment of these patients.

The use of general descriptive names, registered names, trademarks, service marks, etc. in this p... more The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. While the advice and information in this book are believed to be true and accurate at the date of publication, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein.

Effect of hydric stress-related acoustic emission on transcriptional and biochemical changes associated with a water deficit in Capsicum annuum L

Plant Physiology and Biochemistry

At specific vibration frequencies like ones generated by insects such as caterpillar chewing and ... more At specific vibration frequencies like ones generated by insects such as caterpillar chewing and bee's buzz-pollination turn on the plants secondary metabolism and their respective pathways gets activated. Thus, studies report that vibrations and sound waves applied to plants improves their fitness performance. Commonly, acoustic treatments for plants have used arbitrarily random frequencies. In this work, a group of signals obtained from hydric-stressed plants was recorded as vibrational patterns using a laser vibrometer. These vibration-signals were classified as representative of each condition and then externally applied as Acoustic Emission Patterns (AEP). The present research hypothesized that specific vibration frequencies could "emulate" a plant signal through mechanical energy based on tplant's ability to recognize vibration pattern similarity to a hydric status. This investigation aimed to apply the AEP's as characteristic vibrations classified as Low hydric stress (LHS), medium hydric stress (MHS), and high hydric stress (HHS) to evaluate their effect on healthy-well watered plants at two developmental stages. In the vegetative stage, the gene expression related to antioxidant and hydric stress responses was assessed. The LHS, MHS, and HHS acoustic treatments up-regulated the peroxidase (Pod) (~2.8, 1.9, and 3.6-fold change, respectively). The superoxide dismutase (Mn-sod) and phenylalanine ammonia-lyase (Pal) genes were up-regulated by HHS (~0.23 and ~0.55-fold change, respectively) and, the chalcone synthase (Chs) gene was induced by MHS (~0.63-fold-change). At the fructification stage, the MHS treatment induced a significant increase in Capsaicin content (5.88-fold change), probably through the at3and kas gene activation. Findings are correlated for a better understanding of plant responses to different multi frequency-signals tones from vibrations with potential for agricultural applications.

Elicitation with Nanomaterials in the Early Stages of Plants

Industrial Crops and Products, 2019

Stevia rebaudiana (Bertoni) is a perennial shrub native from Paraguay whose leaves accumulate ste... more Stevia rebaudiana (Bertoni) is a perennial shrub native from Paraguay whose leaves accumulate steviol glycosides. Plants increase the production of phytochemicals as a response to stress factors to cope with possible damage. Elicitors are biological stress factors that can induce plant response to stress. Steviol glycosides are the phytochemicals responsible of the sweetener power of stevia (Stevia rebaudiana Bertoni cv. Morita II), which is up to 300-fold stronger than sucrose. Elicitors might affect Steviol glycosides content and gene expressionassociated to their biosynthesis. The aim of this research was to evaluate the effect of controlled elicitation using salicylic acid, chitosan and hydrogen peroxide on plant performance, steviol glycosides contents and gene expression-associated to steviol glycosides biosynthesis (SrKA13H, SrUGT85C2, SrUGT74G1 and SrUGT76G1) in stevia cultivated under greenhouse conditions. Weekly applications of elicitors were foliarly sprayed during four weeks, morphological measurements were made and samples were lyophilized and processed to evaluate SGs content. Gene expression-associated was analyzed by Ultra-Performance Liquid Chromatography coupled to a Quadrupole-Time of Flight. Results showed that all the evaluated elicitors (especially salicylic acid 0.1 mM) enhanced steviol glycosides content, which correlated with inducement of gene expression-associated to the biosynthesis of these compounds and increase in leaf number in stevia. Thus, controlled elicitation of stevia cultivation might be used to improve the yield of steviol glycosides for industrial purposes.

Asian Journal of Plant Sciences, 2008

www.mdpi.com/journal/ijms Transcriptomic Analysis in Diabetic Nephropathy of Streptozotocin-Induced Diabetic Rats

Abstract: Diabetic nephropathy (DN) is a major complication of diabetes and is caused by an imbal... more Abstract: Diabetic nephropathy (DN) is a major complication of diabetes and is caused by an imbalance in the expression of certain genes that activate or inhibit vital cellular functions of kidney. Despite several recent advances, the pathogenesis of DN remains far from clear, suggesting the need to carry out studies identifying molecular aspects, such as gene expression, that could play a key role in the development of DN. There are several techniques to analyze transcriptome in living organisms. In this study, the suppression subtractive hybridization (SSH) method was used to generate up- and down-regulated subtracted cDNA libraries in the kidney of streptozotocin (STZ)-induced diabetic rats. Northern-blot analysis was used to confirm differential expression ratios from the obtained SSH clones to identify genes related to DN. 400 unique SSH clones were randomly chosen

Functional Plant Biology, 2018

Damage-associated molecular patterns (DAMPs) have been studied recently to understand plant self–... more Damage-associated molecular patterns (DAMPs) have been studied recently to understand plant self–nonself recognition in response to attack by biotic and abiotic stresses. Extracellular DNA has emerged as a possible DAMP. As a DAMP DNA seems to function depending on the phylogenetic scale and has been tested in a few plant species. This study aimed to evaluate the possible role of self DNA (sDNA) as a DAMP by analysing changes in CpG DNA methylation and defence-related responses in lettuce (Lactuca sativa L.) as a model plant. sDNA and nonself DNA (nsDNA) from Capsicum chinense Murray (both species belong to the same clade, Asterids) stimulated aberrant seed germination and root growth in lettuce seedlings. Similar resultswere obtained with nsDNA obtained from Acaciella angustissima (Mill.) Britton & Rose plants (belonging to the clade Rosids I), although at significantly higher concentrations. Moreover, in most cases, this behaviour was correlated with hypomethylation of CpG DNA as ...

Aflatoxins - Biochemistry and Molecular Biology, 2011

β-Carotene Production under Greenhouse Conditions

Nova Science Publishers eBooks, 2009

β-carotene is a secondary metabolite that is a hydrocarbon carotene predominantly located in lowe... more β-carotene is a secondary metabolite that is a hydrocarbon carotene predominantly located in lower concentrations in PS II functioning as a helper to harvest light pigment during photosynthesis and to dissipate excess energy before damage occurs. As other carotenes, β-carotene is uniquely synthesized in plants, algae, fungi and bacteria. βcarotene is the main diet precursor of pro-vitamin A. Additionally, β-carotene serves as an essential nutrient and is in high demand in the market as a natural food colouring agent, as an additive to cosmetics and also as a health food. Several approaches have been carried out in order to increase β-carotene production in algae, bacteria, fungi and plants using biotechnological and engineering focuses. In the case of plants, such species as the tomato have important β-carotene contents, which are theoretically amenable to management using approaches such as fertilization, growth conditions and mild stress. On the other hand, greenhouse structures can protect crops from wind and rain, and can also protect from insects when fitted with insect exclusion screens. β-carotene production could potentially be improved and enhanced in greenhouse conditions in plants as well as in algae, based on the exclusion of the structure, and the possibility of controlling aspects such as climate, fertilization and stress management, among others. Production of βcarotene (and other secondary metabolites) from several organisms in greenhouse conditions should be an interesting future approach, visualizing the greenhouse as a “factory” in frontier technologies such as biotechnology and mechatronics in order to optimize this production