Irineo Torres Pacheco - Academia.edu (original) (raw)

Papers by Irineo Torres Pacheco

Plant Physiology and Biochemistry

At specific vibration frequencies like ones generated by insects such as caterpillar chewing and ... more At specific vibration frequencies like ones generated by insects such as caterpillar chewing and bee's buzz-pollination turn on the plants secondary metabolism and their respective pathways gets activated. Thus, studies report that vibrations and sound waves applied to plants improves their fitness performance. Commonly, acoustic treatments for plants have used arbitrarily random frequencies. In this work, a group of signals obtained from hydric-stressed plants was recorded as vibrational patterns using a laser vibrometer. These vibration-signals were classified as representative of each condition and then externally applied as Acoustic Emission Patterns (AEP). The present research hypothesized that specific vibration frequencies could "emulate" a plant signal through mechanical energy based on tplant's ability to recognize vibration pattern similarity to a hydric status. This investigation aimed to apply the AEP's as characteristic vibrations classified as Low hydric stress (LHS), medium hydric stress (MHS), and high hydric stress (HHS) to evaluate their effect on healthy-well watered plants at two developmental stages. In the vegetative stage, the gene expression related to antioxidant and hydric stress responses was assessed. The LHS, MHS, and HHS acoustic treatments up-regulated the peroxidase (Pod) (~2.8, 1.9, and 3.6-fold change, respectively). The superoxide dismutase (Mn-sod) and phenylalanine ammonia-lyase (Pal) genes were up-regulated by HHS (~0.23 and ~0.55-fold change, respectively) and, the chalcone synthase (Chs) gene was induced by MHS (~0.63-fold-change). At the fructification stage, the MHS treatment induced a significant increase in Capsaicin content (5.88-fold change), probably through the at3and kas gene activation. Findings are correlated for a better understanding of plant responses to different multi frequency-signals tones from vibrations with potential for agricultural applications.

Industrial Crops and Products, 2019

Stevia rebaudiana (Bertoni) is a perennial shrub native from Paraguay whose leaves accumulate ste... more Stevia rebaudiana (Bertoni) is a perennial shrub native from Paraguay whose leaves accumulate steviol glycosides. Plants increase the production of phytochemicals as a response to stress factors to cope with possible damage. Elicitors are biological stress factors that can induce plant response to stress. Steviol glycosides are the phytochemicals responsible of the sweetener power of stevia (Stevia rebaudiana Bertoni cv. Morita II), which is up to 300-fold stronger than sucrose. Elicitors might affect Steviol glycosides content and gene expressionassociated to their biosynthesis. The aim of this research was to evaluate the effect of controlled elicitation using salicylic acid, chitosan and hydrogen peroxide on plant performance, steviol glycosides contents and gene expression-associated to steviol glycosides biosynthesis (SrKA13H, SrUGT85C2, SrUGT74G1 and SrUGT76G1) in stevia cultivated under greenhouse conditions. Weekly applications of elicitors were foliarly sprayed during four weeks, morphological measurements were made and samples were lyophilized and processed to evaluate SGs content. Gene expression-associated was analyzed by Ultra-Performance Liquid Chromatography coupled to a Quadrupole-Time of Flight. Results showed that all the evaluated elicitors (especially salicylic acid 0.1 mM) enhanced steviol glycosides content, which correlated with inducement of gene expression-associated to the biosynthesis of these compounds and increase in leaf number in stevia. Thus, controlled elicitation of stevia cultivation might be used to improve the yield of steviol glycosides for industrial purposes.

Asian Journal of Plant Sciences, 2008

Abstract: Diabetic nephropathy (DN) is a major complication of diabetes and is caused by an imbal... more Abstract: Diabetic nephropathy (DN) is a major complication of diabetes and is caused by an imbalance in the expression of certain genes that activate or inhibit vital cellular functions of kidney. Despite several recent advances, the pathogenesis of DN remains far from clear, suggesting the need to carry out studies identifying molecular aspects, such as gene expression, that could play a key role in the development of DN. There are several techniques to analyze transcriptome in living organisms. In this study, the suppression subtractive hybridization (SSH) method was used to generate up- and down-regulated subtracted cDNA libraries in the kidney of streptozotocin (STZ)-induced diabetic rats. Northern-blot analysis was used to confirm differential expression ratios from the obtained SSH clones to identify genes related to DN. 400 unique SSH clones were randomly chosen

Functional Plant Biology, 2018

Damage-associated molecular patterns (DAMPs) have been studied recently to understand plant self–... more Damage-associated molecular patterns (DAMPs) have been studied recently to understand plant self–nonself recognition in response to attack by biotic and abiotic stresses. Extracellular DNA has emerged as a possible DAMP. As a DAMP DNA seems to function depending on the phylogenetic scale and has been tested in a few plant species. This study aimed to evaluate the possible role of self DNA (sDNA) as a DAMP by analysing changes in CpG DNA methylation and defence-related responses in lettuce (Lactuca sativa L.) as a model plant. sDNA and nonself DNA (nsDNA) from Capsicum chinense Murray (both species belong to the same clade, Asterids) stimulated aberrant seed germination and root growth in lettuce seedlings. Similar resultswere obtained with nsDNA obtained from Acaciella angustissima (Mill.) Britton & Rose plants (belonging to the clade Rosids I), although at significantly higher concentrations. Moreover, in most cases, this behaviour was correlated with hypomethylation of CpG DNA as ...

Aflatoxins - Biochemistry and Molecular Biology, 2011

Nova Science Publishers eBooks, 2009

β-carotene is a secondary metabolite that is a hydrocarbon carotene predominantly located in lowe... more β-carotene is a secondary metabolite that is a hydrocarbon carotene predominantly located in lower concentrations in PS II functioning as a helper to harvest light pigment during photosynthesis and to dissipate excess energy before damage occurs. As other carotenes, β-carotene is uniquely synthesized in plants, algae, fungi and bacteria. βcarotene is the main diet precursor of pro-vitamin A. Additionally, β-carotene serves as an essential nutrient and is in high demand in the market as a natural food colouring agent, as an additive to cosmetics and also as a health food. Several approaches have been carried out in order to increase β-carotene production in algae, bacteria, fungi and plants using biotechnological and engineering focuses. In the case of plants, such species as the tomato have important β-carotene contents, which are theoretically amenable to management using approaches such as fertilization, growth conditions and mild stress. On the other hand, greenhouse structures can protect crops from wind and rain, and can also protect from insects when fitted with insect exclusion screens. β-carotene production could potentially be improved and enhanced in greenhouse conditions in plants as well as in algae, based on the exclusion of the structure, and the possibility of controlling aspects such as climate, fertilization and stress management, among others. Production of βcarotene (and other secondary metabolites) from several organisms in greenhouse conditions should be an interesting future approach, visualizing the greenhouse as a “factory” in frontier technologies such as biotechnology and mechatronics in order to optimize this production

On a world basis, water availability is considered the main constraint for crop production. Conce... more On a world basis, water availability is considered the main constraint for crop production. Concerns about water accessibility have always accompanied crop production in dry areas, which are on the other hand the most extensive areas for agriculture on the earth. As a consequence, men had to develop agricultural strategies to cope with water shortage, growing the plants during the short climatic interval of water availability and selecting plants possessing a relatively superior tolerance to water deficiency. Although development of drought tolerant plants by conventional breeding methods has resulted in modest

2017 XIII International Engineering Congress (CONIIN), 2017

The study of the rhizosphere with conventional methods can lead to errors in the analysis of the ... more The study of the rhizosphere with conventional methods can lead to errors in the analysis of the soil-root interface. The aim of this work is to design and carry out a versatile Rhizobox system that allows the study of different crops and types of research. For this purpose, a model that allows different adjustments in the support and development compartments of the rhizosphere and an array of access points in the lower compartment to easy aquare samples in situ were designed. The above, aims to generate an information platform that basis to generate strategies for performance of crops.

Revista Mexicana de Ciencias Agrícolas, Sep 4, 2017

El cáncer bacteriano causado por Clavibacter michiganensis ssp. michiganensis (Cmm), es una enfer... more El cáncer bacteriano causado por Clavibacter michiganensis ssp. michiganensis (Cmm), es una enfermedad devastadora del jitomate alrededor del mundo. El objetivo del presente estudio fue describir un procedimiento rápido basado en PCR para la detección de Cmm patogénica en plantas de jitomate. El DNA fue aislado por el método de hervido y se realizó PCR para la amplificación del gen pat-1 (serina proteasa), empleando los oligonucleótidos CMM5F y CMM6R, cuyo tamaño de amplicón fue de 608 pb. El fragmento fue clonado, secuenciado y comparado con la base de datos del NCBI (Centro Nacional para la Información Biotecnológica), mostrando 100% de identidad con secuencias del gen pat-1 de Cmm. Adicionalmente, de la secuencia obtenida se diseñaron 2 pares de oligonucleótidos anidados para producir amplicones de 250 pb a una temperatura de alineamiento de 62 °C. Los resultados descritos en el presente trabajo permitirán la identificación rápida y precisa de Cmm por PCR en semilla y en tejido vegetal de jitomate, al reducir el tiempo de análisis de 24 a 4 h aproximadamente.

Biosystems Engineering: Biofactories for Food Production in the Century XXI, 2014

One of the biggest challenges in the coming years is to reduce the impact of chronic diseases. Un... more One of the biggest challenges in the coming years is to reduce the impact of chronic diseases. Unhealthy eating is an increasing problem and underlies much of the increase in mortality from chronic diseases that are occurring worldwide. Diets based on plant foods are strongly associated with reduced risks in major chronic diseases, but the constituents in plants that promote health have proved difficult to identify. Plant cells have been used, in the past two decades, as efficient host systems with great potential for the expression of foreign proteins and for the production of nonprotein bioactive small molecules. Till date, the systems implemented include a variety of approaches using whole plants, plant tissue cultures, and cell cultures for the synthesis of a myriad of compounds that include foreign protein expression for the production of antibodies and vaccines. Plant cells have also been used as bioreactors for the massive production on plant-derived bioactive small molecules which are important dietary components or used in the industry as pharmaceuticals, pesticides, flavors, and fragrances. Plant genetics and metabolic engineering can be used to make foods that differ only in their content of specific phytonutrients.

world-pepper.org

< 100 > < First World Pepper Convention 2004 > < Primera Convención Mundial del Ch... more < 100 > < First World Pepper Convention 2004 > < Primera Convención Mundial del Chile 2004 > ... INTERACCIÓN ENTRE GEMINIVIRUS-CHILE (CAPSICUM SPP.) EN INFECCIONES SIMPLES ... María del Rosario Gasca González1, Yadira Rivera Herrera1, Carlos Alberto Zamora ...

African Journal of Microbiology Research, 2012

One concern for garlic (Allium sativum Linnaeus) producers is the damage caused by the pathogen S... more One concern for garlic (Allium sativum Linnaeus) producers is the damage caused by the pathogen Sclerotium cepivorum Berk. Unfortunately, the genetic and molecular mechanisms that participate during S. cepivorum Berk pathogenesis are currently unknown. In order to identify and isolate genes that are differentially expressed by the fungus during garlic white rot pathogenesis, PCR-based suppression subtractive hybridization (SSH) was used. Combining SSH and cDNA arrays hybridization techniques, 120 ESTs whose expression is restricted to the pathogenic stage were identified and isolated. Fourteen ESTs showing higher expression in cDNA arrays were sequenced, these included homologues to oxaloacetate acetylhydrolase (Oah), cysteine desulfurase (Nfs 1p), regulator of drug sensitivity (Rds 1p), outer membrane protein (Omp 1) and cell wall adhesion (Fig2p). One selected EST with high homology to Oah gene, a putative virulence factor, was analyzed by RT-PCR. The possible role of Oah gene in the pathogenesis of this fungus toward garlic is discussed. The combined applications of SSH and cDNA arrays permitted global analysis of gene expression patterns in S. cepivorum Berk, as an initial stage to improve the knowledge at molecular level from fungal pathogenesis.

AFRICAN JOURNAL OF BIOTECHNOLOGY, 2012

The effects of climate change on plant diseases have been the subject of intense debate in the la... more The effects of climate change on plant diseases have been the subject of intense debate in the last decade; research in this sense has been carry out, however, more information is needed. Elevated temperatures and carbon dioxide concentrations associated with climate change will have a substantial impact on plant-disease interactions. Changes in temperature affect both the host and the pathogen; thus, risk analyses must be conducted for each pathosystem to determine the effects of climate change. Studies have been performed under controlled conditions, and the effects of high CO 2 levels have been identified; however, field responses such as the adaptation of pathogens over time may be different. The climate influences the incidence as well as temporal and spatial distribution of plant diseases. The most likely effect of climate change in poleward modifies agroclimates zones, this causing a shift in the geographical distribution of host pathogens. Considering this climate change could profoundly affect the status of agricultural diseases, the focus of this study was to review studies related to the effects of climate change on plant diseases. Taking into account the work done, this review addresses the impact of climate change on plant diseases, considering the effect on crop grown, development and the impact on crop production.

Sustainability

Focusing on new food production methods and sustainable pillars’ accomplishments has changed the ... more Focusing on new food production methods and sustainable pillars’ accomplishments has changed the definition of sustainable pillars themselves. Moreover, some general characteristics of the main pillars can be redefined in separate dimensions to better explain their positive sustainable impacts. Therefore, the main objective of this research is to redefine the sustainable pillars linked to food production and review the most important cultural and technological sustainability impacts they have, in addition to the three classic pillars: economic, social, and environmental sustainability. Cultural and technological sustainability are increasingly important complements to the traditional sustainability concept. Furthermore, new food production technologies and systems are influenced by ancient production methods, as well as by profitable crop selection. Traditional agricultural and aquaculture production in relation to more recent aquaponic production concepts are still a major part of ...

European Journal of Plant Pathology

Abstract: A germin-like gene (CchGLP) cloned from geminivirus-resistant pepper (Capsicum chinense... more Abstract: A germin-like gene (CchGLP) cloned from geminivirus-resistant pepper (Capsicum chinense Jacq. Line BG-3821) was characterized and the enzymatic activity of the expressed protein analyzed. The predicted protein consists of 203 amino acids, similar