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Papers by Isabelle Jeusette
Journal of Veterinary Dentistry, Dec 1, 2013
The purpose of this study was to evaluate the subgingival microbiota and determine the most preva... more The purpose of this study was to evaluate the subgingival microbiota and determine the most prevalent periodontal pathogens implicated in feline periodontal disease and to correlate these findings with the clinical periodontal status. Subgingival microbiological samples were taken under sedation from 50 cats with clinical signs of periodontal disease. Pooled paper point samples from 4 selected subgingival sites were cultured on blood agar and on Dentaid-1 medium. Suspected pathogens were identified, subcultured, and preserved. The association between the microbiological findings and the clinical status was studied using correlation coefficients (CC). In addition, cats were stratified in subgroups according to presence of putative pathogens, and comparisons were carried out using unpaired t-test. Three bacterial species were frequently detected including Porphyromonas gulae (86 %), Porphyromonas circumdentaria (70 %) and Fusobacterium nucleatum (90 %). The mean proportion of total flora was high for P. gulae (32.54 %), moderate for P. circundentaria (8.82 %), and low for F. nucleatum (3.96 %). Among the clinical variables, tooth mobility was correlated (CC > 0.50, p < 0.001) with recession, pocket depth, attachment level, gingival index, and calculus index (CC = 0.29, p = 0.04) as well as with total bacterial counts (CC = 0.38, p = 0.006). Cats with more than 10 % of P. gulae showed significantly more mobility (p = 0.014) and recession (p = 0.038), and a tendency for deeper probing pocket depths (p = 0.084) and attachment loss (p = 0.087). The results from this cross-sectional study confirmed that P. gulae is the most relevant pathogen in periodontal disease in cats.
ABSTRACT The objective of this study was to evaluate the effects of ovariectomy (i) and ad libitu... more ABSTRACT The objective of this study was to evaluate the effects of ovariectomy (i) and ad libitum feeding (ii) on energy intake, body weight (BW), body composition, thyroid status, leptin and ghrelin plasma concentrations. Four young adult female Beagle dogs were fed a maintenance diet for 6 weeks prior to ovariectomy, then 6 months after. Food allowance was adjusted in order to maintain optimal BW. Then, a diet slightly higher in energy concentration was fed ad libitum for 4 months. The maintenance diet was then fed ad libitum for one additional month. The maintenance of optimal BW after ovariectomy required a significant decrease in energy allowance. No increase in fat mass was observed. Ghrelin concentration remained unchanged. During the first month of ad libitum feeding, plasma ghrelin concentration and energy intake increased, then they decreased. Mean BW, plasma leptin, thyrotropin (TSH), total triiodothyronine (TT3) and total thyroxine (TT4) concentrations significantly increased over the study. The BW increase was exclusively due to an increase in body fat. In conclusion, energy allowance should be strictly controlled in spayed female dogs. The results suggest that in dogs, thyroid hormones, leptin and ghrelin concentrations change in response to a positive energy balance in an attempt to limit weight gain. However, the significant weight gain shows that this goal was not achieved.
Annales De Medecine Veterinaire, 2001
Journal of Veterinary Research
Introduction Escherichia coli is the most common pathogen isolated from the urine of dogs with ur... more Introduction Escherichia coli is the most common pathogen isolated from the urine of dogs with urinary tract infections (UTIs). While there are many studies in humans investigating the potential for the prevention of UTIs by dietary consumption of cranberry, few analogous studies have been carried out in dogs. Material and Methods Eight dogs, four male and four female, were successively fed two diets, first a control without cranberry, and then the second diet containing cranberry extracts. Naturally excreted urine was collected on the tenth day after the start of each diet for 24 h and used for bacterial growth. Madin-Darby canine kidney cell adherence by the uropathogenic E. coli G1473 strain expressing type 1 pili and positive for P pili and haemolysin gene markers was quantified after growth in urine samples. Results Significant reductions in bacterial adherence to MDCK cells (from −16.5 to −73.4%, P < 0.05) were observed in the four females but not in the males after consump...
digestibility is reduced in old cats with subnormal
Journal of Veterinary Behavior, 2021
Abstract To evaluate (1) the effects of 3 mild stressors on urinary cortisol––a stress marker––in... more Abstract To evaluate (1) the effects of 3 mild stressors on urinary cortisol––a stress marker––in cats and to evaluate; (2) the effects on this parameter of a new prescription diet (diet US) with L-tryptophan, lemon balm, oligofructose, and fish peptides compared with a control prescription diet (Diet U) with L-tryptophan alone. Ten colony cats were included in the study. Baseline measurements were first obtained with cats fed a baseline diet without nutraceutical to validate the effects of 3 mild stressors (open field test, overnight fast, and blood sampling) on urinary cortisol. Twenty-four-hour free catch urine specimens were obtained under routine management conditions and following application of the three mild stressors. Cats were then randomized in 2 groups to test diet U or US for 5 weeks in a crossover design, following the same procedure as for baseline measurements. Twenty-four-hour urinary cortisol/creatinine ratio, serum cortisol and serum serotonin were measured. At baseline, open field test and blood sampling induced greater 24-hour urinary cortisol/creatinine ratio compared to the value obtained under routine management while overnight fasting did not. In the crossover design, compared to diet U, diet US resulted in a lower average 24-hour urinary cortisol/creatinine ratio. Open field test and blood sampling can be considered as mild stressors in cats. Compared to a diet with L-tryptophan alone, a diet supplemented with lemon balm, fish peptides, oligofructose, and L-tryptophan resulted in a lower average 24-hour urinary cortisol/creatinine ratio, a marker of stress, in cats.
1. Labo diervoeding, Faculteit Diergeneeskunde, Universiteit Gent, Belgie 2. Affinity-Petcare, Ba... more 1. Labo diervoeding, Faculteit Diergeneeskunde, Universiteit Gent, Belgie 2. Affinity-Petcare, Barcelona, Spanje 3. Metabool labo, Universitair Ziekenhuis Gent, Belgie 4. Labo voor diervoeding en kwaliteit van dierlijke producten, Universiteit Gent, Belgie 5. Voeding en Endocrinologie Unit, Nantes-Atlantic National College of Veterinary Medicine, UNAM Universiteit, Food Science and EngineeringONIRIS, Frankrijk Wendy.Wambacq@ugent.be
Annales De Medecine Veterinaire, 2000
Journal of Veterinary Dentistry, Dec 1, 2013
The purpose of this study was to evaluate the subgingival microbiota and determine the most preva... more The purpose of this study was to evaluate the subgingival microbiota and determine the most prevalent periodontal pathogens implicated in feline periodontal disease and to correlate these findings with the clinical periodontal status. Subgingival microbiological samples were taken under sedation from 50 cats with clinical signs of periodontal disease. Pooled paper point samples from 4 selected subgingival sites were cultured on blood agar and on Dentaid-1 medium. Suspected pathogens were identified, subcultured, and preserved. The association between the microbiological findings and the clinical status was studied using correlation coefficients (CC). In addition, cats were stratified in subgroups according to presence of putative pathogens, and comparisons were carried out using unpaired t-test. Three bacterial species were frequently detected including Porphyromonas gulae (86 %), Porphyromonas circumdentaria (70 %) and Fusobacterium nucleatum (90 %). The mean proportion of total flora was high for P. gulae (32.54 %), moderate for P. circundentaria (8.82 %), and low for F. nucleatum (3.96 %). Among the clinical variables, tooth mobility was correlated (CC > 0.50, p < 0.001) with recession, pocket depth, attachment level, gingival index, and calculus index (CC = 0.29, p = 0.04) as well as with total bacterial counts (CC = 0.38, p = 0.006). Cats with more than 10 % of P. gulae showed significantly more mobility (p = 0.014) and recession (p = 0.038), and a tendency for deeper probing pocket depths (p = 0.084) and attachment loss (p = 0.087). The results from this cross-sectional study confirmed that P. gulae is the most relevant pathogen in periodontal disease in cats.
ABSTRACT The objective of this study was to evaluate the effects of ovariectomy (i) and ad libitu... more ABSTRACT The objective of this study was to evaluate the effects of ovariectomy (i) and ad libitum feeding (ii) on energy intake, body weight (BW), body composition, thyroid status, leptin and ghrelin plasma concentrations. Four young adult female Beagle dogs were fed a maintenance diet for 6 weeks prior to ovariectomy, then 6 months after. Food allowance was adjusted in order to maintain optimal BW. Then, a diet slightly higher in energy concentration was fed ad libitum for 4 months. The maintenance diet was then fed ad libitum for one additional month. The maintenance of optimal BW after ovariectomy required a significant decrease in energy allowance. No increase in fat mass was observed. Ghrelin concentration remained unchanged. During the first month of ad libitum feeding, plasma ghrelin concentration and energy intake increased, then they decreased. Mean BW, plasma leptin, thyrotropin (TSH), total triiodothyronine (TT3) and total thyroxine (TT4) concentrations significantly increased over the study. The BW increase was exclusively due to an increase in body fat. In conclusion, energy allowance should be strictly controlled in spayed female dogs. The results suggest that in dogs, thyroid hormones, leptin and ghrelin concentrations change in response to a positive energy balance in an attempt to limit weight gain. However, the significant weight gain shows that this goal was not achieved.
Annales De Medecine Veterinaire, 2001
Journal of Veterinary Research
Introduction Escherichia coli is the most common pathogen isolated from the urine of dogs with ur... more Introduction Escherichia coli is the most common pathogen isolated from the urine of dogs with urinary tract infections (UTIs). While there are many studies in humans investigating the potential for the prevention of UTIs by dietary consumption of cranberry, few analogous studies have been carried out in dogs. Material and Methods Eight dogs, four male and four female, were successively fed two diets, first a control without cranberry, and then the second diet containing cranberry extracts. Naturally excreted urine was collected on the tenth day after the start of each diet for 24 h and used for bacterial growth. Madin-Darby canine kidney cell adherence by the uropathogenic E. coli G1473 strain expressing type 1 pili and positive for P pili and haemolysin gene markers was quantified after growth in urine samples. Results Significant reductions in bacterial adherence to MDCK cells (from −16.5 to −73.4%, P < 0.05) were observed in the four females but not in the males after consump...
digestibility is reduced in old cats with subnormal
Journal of Veterinary Behavior, 2021
Abstract To evaluate (1) the effects of 3 mild stressors on urinary cortisol––a stress marker––in... more Abstract To evaluate (1) the effects of 3 mild stressors on urinary cortisol––a stress marker––in cats and to evaluate; (2) the effects on this parameter of a new prescription diet (diet US) with L-tryptophan, lemon balm, oligofructose, and fish peptides compared with a control prescription diet (Diet U) with L-tryptophan alone. Ten colony cats were included in the study. Baseline measurements were first obtained with cats fed a baseline diet without nutraceutical to validate the effects of 3 mild stressors (open field test, overnight fast, and blood sampling) on urinary cortisol. Twenty-four-hour free catch urine specimens were obtained under routine management conditions and following application of the three mild stressors. Cats were then randomized in 2 groups to test diet U or US for 5 weeks in a crossover design, following the same procedure as for baseline measurements. Twenty-four-hour urinary cortisol/creatinine ratio, serum cortisol and serum serotonin were measured. At baseline, open field test and blood sampling induced greater 24-hour urinary cortisol/creatinine ratio compared to the value obtained under routine management while overnight fasting did not. In the crossover design, compared to diet U, diet US resulted in a lower average 24-hour urinary cortisol/creatinine ratio. Open field test and blood sampling can be considered as mild stressors in cats. Compared to a diet with L-tryptophan alone, a diet supplemented with lemon balm, fish peptides, oligofructose, and L-tryptophan resulted in a lower average 24-hour urinary cortisol/creatinine ratio, a marker of stress, in cats.
1. Labo diervoeding, Faculteit Diergeneeskunde, Universiteit Gent, Belgie 2. Affinity-Petcare, Ba... more 1. Labo diervoeding, Faculteit Diergeneeskunde, Universiteit Gent, Belgie 2. Affinity-Petcare, Barcelona, Spanje 3. Metabool labo, Universitair Ziekenhuis Gent, Belgie 4. Labo voor diervoeding en kwaliteit van dierlijke producten, Universiteit Gent, Belgie 5. Voeding en Endocrinologie Unit, Nantes-Atlantic National College of Veterinary Medicine, UNAM Universiteit, Food Science and EngineeringONIRIS, Frankrijk Wendy.Wambacq@ugent.be
Annales De Medecine Veterinaire, 2000