Yulia Ismail - Academia.edu (original) (raw)

Papers by Yulia Ismail

Journal of Physics: Conference Series, 2019

Vibriosis is one of the major problems in prawn aquaculture leading to a significant loss in yiel... more Vibriosis is one of the major problems in prawn aquaculture leading to a significant loss in yield. The objective of the study was to isolate and identify the etiological agent of vibriosis from the giant freshwater prawn (Macrobrachium rosenbergiii) aquaculture. The study was conducted by isolating the bacteria using selective media of thiosulfate-citrate-bile salts-sucrose (TCBS) agar followed by the morphological and biochemical characterizations, i.e., indole, methyl-red (MR), Voges-Proskauer (VP), citrate, urease, NaCl 0%, gelatinase and lysine decarboxylase tests. The isolated bacteria were further assayed for their standard growth-curve as well as the ability to form a biofilm on Congo-red agar (CRA). The results showed that the bacteria showed a yellow color in colony appearance and had negative Gram staining. On the biochemical assays, the isolates showed positive reactions on the indole, MR, VP, gelatine, and lysine decarboxylase; but had negative reactions on the citrate,...

Biodiversitas, Sep 3, 2019

IOP Conference Series: Earth and Environmental Science

The use of probiotics in aquaculture can improve the quality and quantity of the fishes. One of p... more The use of probiotics in aquaculture can improve the quality and quantity of the fishes. One of potential probiotic bacterium from gastrointestine of mackerel fish (Rastrelliger sp.) isolated in this research were LUI-04. This study aimed to determine the species of bacterium isolate using nucleotide sequence homology compared to other microorganisms in GenBank. The 16S rRNA genes of LUI-04 isolate were analyzed. The results of electrophoresis showed the DNA band had a size of 1 500 bp for amplification using Bact-27F and Uni-1492R primers, and about 250 bp to 500 bp with the Com-IF and Com-2R primers. The sequence of nucleotides was determined by Sanger method. The 16S rRNA sequence of the isolate and other bacteria in GenBank were compared with the Basic Alignment Search Tool (BLAST) to know the homology. Then, all of the sequences were aligned by ClustalW, and the phylogenetic tree was reconstructed with Neighbor Joining method using bootstrap analysis of 1 000 replicates. The re...

IOP Conference Series: Earth and Environmental Science, 2021

During the long course of evolution, disease-causing pathogenic bacteria have developed a variety... more During the long course of evolution, disease-causing pathogenic bacteria have developed a variety of virulence mechanisms that help them establish and maintain infections. Among these mechanisms are the production of biofilm protecting the bacteria from undesirable environmental conditions and contributing to the development of new multi-drug-resistance bacterial strains. Hence, biofilm has become an attracted target for the development of a novel strategy in fighting against biofilm-forming pathogens. There has been much work to look for novel antibacterial and antibiofilm agents, including the use of plant-derived materials. Hibiscus tiliaceus is one of the widely known medicinal plant with antibacterial and antibiofilm properties. This review focuses on antibacterial and antibiofilm properties of H. tiliaceus as well as the major phytochemical constituents that might contribute to these activities.

Journal of Physics: Conference Series, Oct 1, 2019

Vibriosis is still a major threat in aquaculture generating significant implications in ecology a... more Vibriosis is still a major threat in aquaculture generating significant implications in ecology and economy. One of the ethologic agents of vibriosis is Vibrio alginolyticus that infects shrimp and fish aquaculture through biofilm-mediated. The objective of the study was to determine the biopotency of ethanolic extract of waru (Hibiscus tiliaceus) leaves to inhibit biofilm formation of V. alginolyticus in vitro. A microtiter plate biofilm assay (OD 570) method was applied in this study. The treatments were the addition of ethanolic leaves extracts of waru (H. tiliaceus) at different concentrations of 2%, 4%, 8%, and 10%. The values of biofilm inhibition activity were measured as optical density data that were statistically analyzed using One Way ANOVA, followed by Tukey's test and the tests were considered statistically significant at a P ≤ 0.05 on two-tailed. The result of this study showed that Waru leaves extract significantly inhibited the biofilm formation of V. alginolyticus. The best inhibition was shown at 10% concentration of extract signifying the potential application of waru to treat biofilmmediated diseases in aquaculture.

Of aquatic environment, antibiotic resistant bacteria, including total coliforms and E. coli diss... more Of aquatic environment, antibiotic resistant bacteria, including total coliforms and E. coli disseminate and emerge at an alarming rate. The study aims to determine enumerate, isolate,E. coliand determine their antibiotic resistance and compare between those which were recovered from residentials and home industries in Banda Aceh and its surrounding area. The bacterial density and antibiotic susceptibility of total coliforms and E. coli were determined using Standard Total Coliform Multiple-Tube (MPN) Fermentation method and the disk diffusion method, respectively. Despite there was no significant difference of total coliforms and E. coli population between residentials and home industries (P > 0.05) in this study, their density as well as prevalence remained high in the water sample. This might expose serious health risks since the resistance might be easily spread acquired through horizontal gene transfer within the aquatic environment.

IOP Conference Series: Earth and Environmental Science

Vibriosis caused by Vibrio alginolyticus infection is getting challenging to treat as the bacteri... more Vibriosis caused by Vibrio alginolyticus infection is getting challenging to treat as the bacteria harbor the ability to form biofilm. One of the natural products that might be potentially applied to treat vibriosis through biofilm deformation is the leaves of Moringa oleifera. The objective of the study was to determine the effect of ethanol extracts of M. oleifera to inhibit V. alginolyticus biofilm formation in vitro. A microtiter plate biofilm assay (OD570nm) method was applied in this study. The treatments were the addition of ethanolic Moringa leaves extracts at different concentrations of 2%, 4%, 8% and 10%. The result of this study showed that Moringa leaves extract significantly inhibited the biofilm formation of V. alginolyticus. The optimal condition to inhibit biofilm formation was at 10% concentration for 60 hours incubation at room temperature signifying the potential application of Moringa leaf extracts to treat biofilmmediated diseases in aquaculture.

Biodiversitas Journal of Biological Diversity

The occurrence of pathogenic bacteria in an aquatic system might increase waterborne infections i... more The occurrence of pathogenic bacteria in an aquatic system might increase waterborne infections including salmonellosis. This might raise a concern related to the increasing pathogenic bacteria, including the multidrug-resistant Salmonella spp. in the two major rivers, namely Lamnyong and Krueng Aceh crossing the city of Banda Aceh, Indonesia. The objectives of the present study were to isolate and enumerate Salmonella spp. recovered from aquatic environment in Banda Aceh and to determine their antibiotic susceptibility. Bacterial enumeration was performed using most probable number (MPN) method followed by bacterial isolations using selective media Salmonella-Shigella Agar (SSA); whereas, antibiotic susceptibility test was conducted using a procedure as recommended by the National Committee for Clinical Laboratory Standards. There was no significant Salmonella spp. densities/mL of both locations (p = 0.200) although, on average there was about 441.67 Salmonella spp. MPN/mL in Krueng Aceh as opposed to 20.47 MPN/mL of Lamnyong river. There were 57 Salmonella spp. isolates recovered in the sampling sites, i.e. 38 isolates of Lamnyong and 19 isolates of Krueng Aceh. The results of antibiotic susceptibility tests showed that Salmonella spp. isolates recovered from Lamnyong and Krueng Aceh showed antibiotic resistance to azithromycin, tetracycline, and streptomycin. Moreover, Salmonella spp. isolates also showed high resistance to ofloxacin, ciprofloxacin, dan ceftriaxone. All Salmonella spp. isolates were classified as MDR with the number of isolates resistant to 4-6 antibiotics was 33 (57.9%), whereas those that were resistant to 7-9 antibiotics and over 10 antibiotics were 23 (40.4%%) and 5 (8.8%) respectively. The prevalence of MDR Salmonella spp. in the current study suggest that river remain the major reservoirs for the transmission of the pathogen in aquatic systems. An appropriate assessment and monitoring are necessary to reduce the potential health risks and to ensure water quality in the aquatic environment.

Biosaintifika: Journal of Biology & Biology Education

Endophytic bacteria are microorganisms that thrive in plant tissue without causing any negative e... more Endophytic bacteria are microorganisms that thrive in plant tissue without causing any negative effects to the host plants. The purpose of this study was to apply endophytic bacteria that have the potential to produce IAA hormones as biological agents in rice seeds. This research was conducted by testing IAA hormone using a spectrophotometer, testing HCN production using filter paper, biochemically identification of the isolate, and applicating endophytic bacteria into rice seeds. Endophytic bacteria have different abilities in producing IAA hormone. Based on the results of the study, the highest IAA concentration (57 ppm) was obtained from KSB1 (Potassium Solubilizing Bacteria) isolates and followed by KSB3 isolate with 56 ppm. All the endophytic bacteria isolates were unable to produce HCN. Results of the study showed that KSB1 and KSB3 isolate were able to influence the growth of root length by 5.62 cm and 6.70 respectively as well as the canopy height by 8.56 cm and 8.30 cm resp...

Biodiversitas Journal of Biological Diversity

The infections attenuated by the presence of virulence factors might be facilitated by quorum sen... more The infections attenuated by the presence of virulence factors might be facilitated by quorum sensing (QS) mechanism. The continuous treatments of bacterial infections with current drugs, however, has developed such pathogenic bacteria more antibiotic-resistant, encouraging to searching for better alternative treatment, such as the use of plant-derived bioactive constituents, including kelor (Moringa oleifera L.). In the present research, kelor (M. oleifera) ethanolic leaf extract was evaluated for their potency as an antibacterial agent to inhibit bacterial virulence factors mediated by quorum s ensing mechanism against Chromobacterium violaceum and Pseudomonas aeruginosa PAO1. A phytochemical examination of M. oleifera leaf-ethanolic extracts has been conducted resulting positive detection of alkaloids, steroids, terpenoids, flavonoids, and phenolics or tannins. The extracts had MIC and MBC values of 10 mg/mL and 20 mg/mL, respectively, for both tested bacteria. The ethanolic extracts of M. oleifera leaves also showed potent inhibition of quorum sensing by significantly reducing violacein and pyocyanin production as well as interference of swarming motility and biofilm formation.

Biodiversitas Journal of Biological Diversity

Proteases are enzymes who catalyze the breakdown of peptide bonds in proteins. This enzyme could ... more Proteases are enzymes who catalyze the breakdown of peptide bonds in proteins. This enzyme could be produced from thermophilic bacteria that are able to grow at temperatures of 45-80ºC and are stable to heat. The samples were collected at Ie Seu'um hot spring, Aceh Besar. This study aimed to isolate, to characterize and to determine actinobacteria that were capable of producing protease enzymes. The sampling method in this study was conducted by purposive sampling at temperatures of 50, 60 and 70ºC. Isolation of thermophilic actinobacteria was carried out in Humic Acid Vitamin B (HV) Agar medium and morphological characterization was carried out in Yeast Malt Agar (YMA), Yeast Starch Agar (YSA), Oatmeal Agar (OA) media. Microscopic characterization and measurement of clear zone diameter formed were carried out in skim milk medium. The results showed that one actinobacteria isolate was obtained at a temperature of 50ºC and five isolates at a temperature of 60ºC, meanwhile, no actinobacteria could be obtained at a temperature of 70ºC. A total of 4 isolates obtained were able to produce protease enzymes. The highest Proteolytic Index (IP) value was obtained from IS01 which was 3.8.

Biosaintifika: Journal of Biology & Biology Education

Endophytic bacteria are beneficial microorganisms that interact with host plants without causing ... more Endophytic bacteria are beneficial microorganisms that interact with host plants without causing any interference or damage to plants. This research aimed to obtain endophytic bacteria isolated from the root tissue of rice plants (Oryza sativa L.) which have potential to produce IAA hormones,, identify the endophytic bacteria in morphologically and physiologically, and analysis of the selected isolate 16S rRNA genes. Based on the results of this research, there were six endophytic bacteria isolates obtained. They have high morphological diversity and differen ability producing IAA hormones. The highest concentration of IAA (425 ppm) was obtained isolates from EAP3. Isolate EAP3 also produce inhibit the growth of Xanthomonas oryzae with a 5.2 mm inhibition zone. Based on the biochemical test, EAP3 had 60% similarity with Enterobacter asburiae. Analysis of the 16S rRNA gene showed that EAP3 had the highest similarity with Enterobacter asburiae strain U4 by 99%. This research data i...

IOP Conference Series: Materials Science and Engineering

The objective of this study was to biosynthesize calcium oxide (CaO) with the employment of the e... more The objective of this study was to biosynthesize calcium oxide (CaO) with the employment of the extract of red dragon fruit peels (Hylocereus polyrhizus) as the biologically reductive agent and to investigate the catalytic performance of the biosynthesized CaO on Candida albicans in aqueous conditions. In the initial process, the biosynthesis was carried out by conditioning the extract of dragon fruit peels reacting with CaCl2.2H2O as a precursor of calcium metal in the system. Continuing the characterization process of the biosynthesized results using Ultraviolet-visible spectroscopy (UV-Vis), it had maximum absorbance at 450 nm, which was indicated by the presence of Ca-O bond in the biosynthesized sample. Another characterization using Fourier-transform infrared spectroscopy (FTIR) showed the typical wave numbers that were observed at 505.35 and 540.07 cm-1. This could be noticed on the IR absorption spectra of CaO crystals. The physical analysis using Scanning electron microscope (SEM) proved the morphology of the biosynthesized CaO which was in the rod shape (fibber), in which the Energy Dispersive X-Ray (EDX) confirmed the contents of the biosynthesized sample. The contents were calcium (29.06%) and oxygen (43,94%). Finally, X-ray diffraction (XRD) characterization showed that the average size of the biosynthesized CaO was 18.98 nm, which means that the produced materials can be classified into Nanoscale based on their sizes. The anti-microorganism activity of the biosynthesized CaO was observed using antifungal experiment against Candida albicans at various concentrations of 4500, 5900 and 6600 μg/mL under turbidimetry method. This indicated its inhibition percentage of 62.2%, 83.5% and 91.8%, respectively. This study revealed that the aqueous extract of the red dragon fruit peels (H. Polyrhizus) was successfully used as biological mediator on the CaO biosynthesis, and the biosynthesized CaO showed inorganic antifungal activity against C. Albicans.

Biodiversitas Journal of Biological Diversity

Endophytic actinobacteria from ginger (Zingiber officinale Rosc.) is a bacterium that is capable ... more Endophytic actinobacteria from ginger (Zingiber officinale Rosc.) is a bacterium that is capable of producing secondary metabolites that are the same as their hosts. This study aims to look at the potential of endophytic actinobacteria from ginger as a pancreatic lipase inhibitor and its toxicity. Endophytic actinobacteria were isolated, purified, then tested for pancreatic lipase inhibitors and their toxicity using the BSLT method (Brine Shrimp Lethality Test) and phytochemical tested on ethanol extract of selected isolates. Seven endophytic actinobacterial isolates were isolated from the ginger rhizome. The isolates had different morphological diversity based on colony and microscopic observations and 5 isolates had pancreatic lipase inhibitor activity. The highest inhibitors were found in AJ4 isolates (89.9%), compared with pancreatic lipase inhibitors crude extracts of ginger (68.9%) and orlistat (88.1%) as positive controls. The LC50 value of AJ4 isolates was 653,381 ppm and the value of LT50 was 17,569 hours. AJ4 isolates contain terpenoids, phenols, tannins, flavonoids, alkaloids, and saponins. This research data is considered as new information about the potential of endophytic actinobacteria from ginger as pancreatic lipase inhibitors and their toxicity.

Biodiversitas Journal of Biological Diversity

Endophytic bacteria live in plant tissues and known to have many benefits for plant growth. One f... more Endophytic bacteria live in plant tissues and known to have many benefits for plant growth. One function of endophytic bacteria are known to be able to dissolve potassium. Potassium is one of the macronutrients that plays an important role in plant growth and development. This study aims to obtain endophytic bacterial isolates from the roots of rice plants (Oryza sativa L.) which have potential as potassium solvents, identify the endophytic bacteria in morphologically and physiologically, and analysis of the selected isolate 16S rRNA genes. Selected endophytic bacterial isolates from the test of dissolving potassium on medium Alexandrov, measured clear zone using calipers and continued with biochemical tests using KIT DL-96E also identified based on 16S rRNA gene and compared for their close relationship with reference strains available in the Bank Genes. Based on the isolation results, 7 isolates from the roots of rice were obtained. Six isolates of endophytic bacteria were able to dissolve potassium. EPK3 isolates were the isolates that are capable of dissolving the highest potassium in Alexandrov media with a 17.9 mm potassium dissolution index. EPK3 isolates also produce inhibit the growth of Xanthomonas oryzae with a 1.2 mm inhibition zone. Physiological identification showed that EPK3 isolates were Enterobacter cloaceae bacteria with 93.79% of similarity. Based on the 16SrRNA gene showed that EPK3 isolates were closely related to E. cloaceae strain SBP-8 with similarity rate is 99%.

Indonesian Journal of Tropical and Infectious Disease

Human Immunodeficiency Virus type 1 (HIV-1) known to cause Acquired Immune Deficiency Syndrome (A... more Human Immunodeficiency Virus type 1 (HIV-1) known to cause Acquired Immune Deficiency Syndrome (AIDS) disease are divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with the host can affect the severity of the disease. This study was to analyze HIV-1 subtypes circulatingin HIV/AIDS patients from the East Java region descriptively and to analyze its relationship with clinical stadiums of HIV/AIDS. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia. This study utilited blood plasma from patients who had been tested to be HIV positive who sected treatment to or were reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology ex...

Jurnal Natural, 2012

Human Immunodeficiency Virus type 1 (HIV-1) has been known to cause Acquired Immune Deficiency Sy... more Human Immunodeficiency Virus type 1 (HIV-1) has been known to cause Acquired Immune Deficiency Syndrome (AIDS) disease and has been alaso divided into several subtypes (A,

Jurnal Teknologi dan Industri Pertanian Indonesia, 2015

&lt;p&gt;(Characterization Of Lactit Acid Bacteria Indegenous From Jruek Drien, A... more &lt;p&gt;(Characterization Of Lactit Acid Bacteria Indegenous From Jruek Drien, Aceh Province&lt;/p&gt;&lt;p&gt;&lt;strong&gt;ABSTRACT&lt;/strong&gt;. Jruek drien is traditional fermented Durian. The purpose of this research was to obtain Lactic acid bacteria indigenous from jruek drien for development potential probiotic. The research was conducted at Laboratory of Microbiology, Department of Biology, Faculty of Mathematic and Natural Science of Syiah Kuala University from Februari – Juny 2015. Sample was taken from Aceh Jaya Regency, Aceh Barat Regency, Aceh Barat Daya Regency, Nagan Raya Regency dan Aceh Selatan Regency. The results showed that there are 9 Lactic acid bacteria indigenous isolate from jruek drien.&lt;/p&gt;

Biodiversitas Journal of Biological Diversity

Journal of Physics: Conference Series, 2019

Biodiversitas, Sep 3, 2019

IOP Conference Series: Earth and Environmental Science

IOP Conference Series: Earth and Environmental Science, 2021

Journal of Physics: Conference Series, Oct 1, 2019

IOP Conference Series: Earth and Environmental Science

Biodiversitas Journal of Biological Diversity

Biosaintifika: Journal of Biology & Biology Education

Biodiversitas Journal of Biological Diversity

Biosaintifika: Journal of Biology & Biology Education

IOP Conference Series: Materials Science and Engineering

Biodiversitas Journal of Biological Diversity

Indonesian Journal of Tropical and Infectious Disease

Jurnal Natural, 2012

Jurnal Teknologi dan Industri Pertanian Indonesia, 2015

Biodiversitas Journal of Biological Diversity