Itzhak Binderman - Profile on Academia.edu (original) (raw)

Papers by Itzhak Binderman

Research paper thumbnail of Quantitative MR of the Distal Radius Bone Marrow as a marker of Osteoporosis

Quantitative MR of the Distal Radius Bone Marrow as a marker of Osteoporosis

In-vivo detection of osteoporosis-related changes in the distal radius bone marrow, using MR prot... more In-vivo detection of osteoporosis-related changes in the distal radius bone marrow, using MR protocols, could provide safe and accessible means for screening and monitoring. A clinical trial was performed, including 26 women assigned into three study groups: healthy premenopausal (n=7), healthy postmenopausal (n=10) and osteoporotic postmenopausal (n=9). Fat composition was evaluated using T2 maps, two-compartment model of T1 and Dixon sequence. The osteoporotic group exhibited higher fat content and lower T2 values compared to the healthy premenopausal group. This study provides proof of concept for the use of the distal radius bone marrow as probing site for osteoporosis.

Research paper thumbnail of Bone and biologically compatible materials in dentistry

Bone and biologically compatible materials in dentistry

PubMed, Dec 1, 1991

This review focuses on compatible materials that are either implanted in bone tissue to serve as ... more This review focuses on compatible materials that are either implanted in bone tissue to serve as prosthetic devices or used as bone substitutes. It attempts to evaluate the significance of studies of combined osteoconductive materials with osteoinductive factors, like demineralized bone or its purified components, and osteogenic cells. It is important to note that the term osseointegration, which is still widely used by clinicians to indicate the bioactivity of implants in bone healing, is not substantiated by any controlled basic study. Even pure titanium and hydroxyapatite can interfere with the normal healing of a surgical bone wound. This review emphasizes the three-dimensional structure of bone, intercellular communication, and the response of this system to bone damage and materials.

Research paper thumbnail of The effect of thyrocalcitonin on calcium uptake by isolated bone cells

The effect of thyrocalcitonin on calcium uptake by isolated bone cells

PubMed, Mar 1, 1971

Research paper thumbnail of A modified form for blade type endosteal implant

A modified form for blade type endosteal implant

PubMed, 1972

Research paper thumbnail of Calcium Uptake by Cultured Bone Cells: The Role of Phosphate, Calcitonin and 1,25-(OH)2D3

Calcium Uptake by Cultured Bone Cells: The Role of Phosphate, Calcitonin and 1,25-(OH)2D3

Springer eBooks, 1976

Research paper thumbnail of Partial characterization of a specific high affinity binding macromolecule for 24R,25 dihydroxyvitamin D3 in differentiating skeletal mesenchyme

Biochemical and Biophysical Research Communications, 1982

Research paper thumbnail of 873 Spontaneous Transformation of Cultured Skin Fibroblasts Into Osteoblasts in a Case of Dysplastic Intra Dermal Calcification

Pediatric Research, Apr 1, 1978

Research paper thumbnail of Heterologous tooth transplant. Eight years follow-up

Heterologous tooth transplant. Eight years follow-up

PubMed, 1986

Research paper thumbnail of Topical application of thyrocalcitonin on the exposed pulp of the rat molar

Topical application of thyrocalcitonin on the exposed pulp of the rat molar

PubMed, Sep 1, 1981

Research paper thumbnail of Biological Aspects

Biological Aspects

Springer eBooks, 2019

Research paper thumbnail of Tissue culture of bone cells: mineral transport, calcification and hormonal effects

Tissue culture of bone cells: mineral transport, calcification and hormonal effects

PubMed, Feb 1, 1976

Research paper thumbnail of Effect of dihydrotachysterol on bone induction in ovariectomized rats

Bone and Mineral, Mar 1, 1989

we have demonstrated via marr"v atromat CEH Eld1"TC6 and the aaeoinductive rclpanm IO deminera. l... more we have demonstrated via marr"v atromat CEH Eld1"TC6 and the aaeoinductive rclpanm IO deminera. lized bone @tr (DSMj i.*, ,ilU cvr,icai bu.. &r&f in ,hc ovrrirtamized (CWX) ,a.t MueeLspostop) ispdmarilydue ,oimpaircdo~,eopraS~ni,orcellproliiera,ion. andthatdihydmtachy~tsrol (DHT) marmen, can be protective. In cultured marrow stromal cells from DVX rats. short-lem~ DHT-R, uaggera,ed the already subnormal pattern 01 ma,,ow stromal cell proliferation. However. in DBM grafts. DHT lrealmen, benefited the time-course of mcxnchymal cell DNAsyntheris asmsasurcd by triliated Ihymid,ne incorporation z!+ weagenic cell ma,u,a,irn as measured by alkaline phmphatase mncen,m,ian. and established a r"SSes,ive ,rcnd D ward normalizado" of bone forma,io~minera,iza,ion (24 h %a incorporation). The dam from this animal model infer that DHT could moderate the bone (OS normally ~fcn in ovarieclomized mavia an activation of the arfeapmgenitorcell population. Key wwdm Osreoinduaion; Bone morphogenetie protein; Deminerakzed bone; Dihydmrachywsrol; Alkaline phospharau; Ovaris,amy and cortical bone mass [I ,32.39].

Research paper thumbnail of Evidence for 24,25-dihydroxycholecalciferol receptors in long bones of newborn rats

Biochemical Journal, Apr 15, 1982

Several reports have appeared that suggest that 24,25-dihydroxycholecalciferol has a possible bio... more Several reports have appeared that suggest that 24,25-dihydroxycholecalciferol has a possible biological role in bone formation. We have utilized competition studies, saturation analysis, sucrose-density-gradient sedimentation and DEAE-cellulose chromatography to demonstrate that long bones of vitamin D-depleted newborn rats contain cytoplasmic and possibly nuclear receptors that bind 24,25-dihydroxycholecalciferol with specificity and high affinity (Kd = 1.79nM). Sucrose-densitygradient analysis of the cytoplasmic 24,25-dihydroxycholecalciferol-binding component showed a single binding macromolecule for 24,25-dihydroxycholecalciferol with a sedimentation coefficient of 3.1 S. DEAE-cellulose chromatography showed a [3H]24,25-dihydroxycholecalciferol-macromolecular complex that binds to DEAEcellulose and elutes between 0.15 and 0.21 M-KCI. The finding of 24,25-dihydroxycholecalciferol receptors in long bones of newborn rats suggests a possible involvement of 24,25-dihydroxycholecalciferol in the metabolism of developing skeletal tissues.

Research paper thumbnail of Biochemical effect of mechanical stress on cultured bone cells

Calcified tissue research, Dec 1, 1976

The mode of transmission of mechanical stimuli into biochemical signals has intrigued research wo... more The mode of transmission of mechanical stimuli into biochemical signals has intrigued research workers for many years. Piatier-Piketty et al. [7], Rodan et al. [8], Davidowitch et al. [2] and Hong et al. [4] have described changes in cell membranes and in the amount of intracellular cyclic nucleotides [2, 7, 8] and of prostaglandins [4] produced by different forms of mechanical stress. The present paper reports evidence for the existence of a stimulus-receptor system in which a distortion of the cell membrane by mechanical perturbations initiates specific biochemical changes inside the cell. We have previously reported that cultured cells derived from the embryonal rat periosteum proliferate, differentiate, are metabolically active and retain their specific response to hormonal treatment. By using this system we were able to expose the cells to mechanical stress.

Research paper thumbnail of The cephalization index: A screening device for brain maturity and vulnerability in normal and intrauterine growth retarded newborns

Brain & Development, 1985

Predictive estimates of future neurological maldevelopment as a result of vascular induced intrau... more Predictive estimates of future neurological maldevelopment as a result of vascular induced intrauterine injury are based on the assumption that the body is more affected than the brain resulting in asymmetrical intrauterine growth retarded (IUCR) newborns. The higher the brain:body ratio, the more severe the IUCR process and the greater the risk for the brain to be affected. This prompted us to study in human newborns, a cephalization index based on the ratio of head circumference to body weight to express the degree of brain maturity and possible vulnerability in relation to gestational age. The newborn cephalization index was correlated with neurodevelopment. A trend could be delineated; in the later gestational age, the higher the cephalization index reflecting a greater degree of brain vulnerability, the more severe the clinical pathology; especially the likelihood of cerebral palsy and severe psychomotor retardation. The cephalization index may serve as an additional screening device for high risk intrauterine growth retarded newborns.

In the present study we have investigated the possibility of the use of a ‘“‘cephalization index” (Cl) based on the ratio of head cir- cumference to body weight defined in weeks of immaturity and expressing possible brain vulnerability. The Cl is suggested to serve as an additional screening device to predict normal or adverse brain development in high risk SFD newborns.

Research paper thumbnail of Brain: Body ratio and conceptional age in vascular-induced intrauterine growth retarded rabbits

Brain & Development, 1985

Vascular induced intrauterine growth retardation (IUGR) was achieved by total ligation of approxi... more Vascular induced intrauterine growth retardation (IUGR) was achieved by total ligation of approximately 30% of the placental vessels to half the fetuses in the last third of gestation in pregnant rabbits. A correlation between brain weight, body weight and head circumference was established in fetuses and rabbit pups in the perinatal period. The brain: body ratio in restricted IUGR animals was significantly higher than their homologous normal controls. A cephalization index based on the brain:body ratio is proposed to assess adverse effects on brain maturity in the presence of IUGR induced by placental insufficiency. Harel S, Yavin E, Tomer A, Barak Y, Binderman 1. Brain: body ratio and conceptional age in vascular-induced intrauterine growth retarded rabbits. Accumulation of clinical data in humans has strongly implicated an association between prolonged placental insufficiency from various causes and intrauterine growth retardation (IUGR) resulting in small-for-gestational age (SGA) newborns. Prenatal undernutrition has been extensively studied. Maternal dietary restriction has been shown to affect the brain growth of rats, guinea pigs and humans [I, 2]. Another approach to study this aspect of the problem in animals is to induce IUGR by uterine artery or placental vascular ligation and embolization. The classic work in this field was done by Wigglesworth [3] who experi-From the

Research paper thumbnail of Role of arachidonate in load transduction in bone cells

Role of arachidonate in load transduction in bone cells

Cambridge University Press eBooks, Apr 28, 1994

Research paper thumbnail of Effect of Fluoride on Hypervitaminosis D in Rats

Effect of Fluoride on Hypervitaminosis D in Rats

Journal of Dental Research, May 1, 1966

SYNOPSIS IN INTERLINGUA LE EFFECTO DE FLUORURO SUPER HYPERVITAMINOSIS D IN RATTOS.-Esseva usate r... more SYNOPSIS IN INTERLINGUA LE EFFECTO DE FLUORURO SUPER HYPERVITAMINOSIS D IN RATTOS.-Esseva usate rattos recipiente 0,2 mg de fluoruro per die (i.e., approximativemente le ingestion physiologic pro le ratto), rattos recipiente doses toxic de vitamina D, rattos recipiente vitamina D e fluoruro simultaneemente, e rattos de controlo. Esseva effectuate determinationes de calcium in le sero e le urina e del gravitate specific e del contento de cinere in le femure si ben como su contento de calcium. Le resultatos revelava que fluoruro age contra le syndrome de hypervitaminosis D per reducer usque ad valores quasi normal le concentrationes de calcium in le sero e le urina e etiam que illo preveni le resorption de osso. Es suggestionate que in hypervitaminosis D, le presentia de un augmentate concentration de iones de fluoruro in le liquidos del tissus induce un precipitation de fluoro-apatite.

Research paper thumbnail of The importance of inorganic phosphate for cartilage mineralization in culture

The importance of inorganic phosphate for cartilage mineralization in culture

Bone and Mineral, Apr 1, 1992

Research paper thumbnail of Induction of Osteogenesis by Free Periosteum Autografts

Journal of Dental Research, Sep 1, 1977

There have been numerous attempts to induce bone formation by dead bone, living bone or bone marr... more There have been numerous attempts to induce bone formation by dead bone, living bone or bone marrow tissue. Some of the positive results were interpreted as favoring the hypothesis that transformation of the surrounding connective tissue into bone is induced by osteogenic substances (AmITANI et al, GANN 66:327, 1975

Research paper thumbnail of Quantitative MR of the Distal Radius Bone Marrow as a marker of Osteoporosis

Quantitative MR of the Distal Radius Bone Marrow as a marker of Osteoporosis

In-vivo detection of osteoporosis-related changes in the distal radius bone marrow, using MR prot... more In-vivo detection of osteoporosis-related changes in the distal radius bone marrow, using MR protocols, could provide safe and accessible means for screening and monitoring. A clinical trial was performed, including 26 women assigned into three study groups: healthy premenopausal (n=7), healthy postmenopausal (n=10) and osteoporotic postmenopausal (n=9). Fat composition was evaluated using T2 maps, two-compartment model of T1 and Dixon sequence. The osteoporotic group exhibited higher fat content and lower T2 values compared to the healthy premenopausal group. This study provides proof of concept for the use of the distal radius bone marrow as probing site for osteoporosis.

Research paper thumbnail of Bone and biologically compatible materials in dentistry

Bone and biologically compatible materials in dentistry

PubMed, Dec 1, 1991

This review focuses on compatible materials that are either implanted in bone tissue to serve as ... more This review focuses on compatible materials that are either implanted in bone tissue to serve as prosthetic devices or used as bone substitutes. It attempts to evaluate the significance of studies of combined osteoconductive materials with osteoinductive factors, like demineralized bone or its purified components, and osteogenic cells. It is important to note that the term osseointegration, which is still widely used by clinicians to indicate the bioactivity of implants in bone healing, is not substantiated by any controlled basic study. Even pure titanium and hydroxyapatite can interfere with the normal healing of a surgical bone wound. This review emphasizes the three-dimensional structure of bone, intercellular communication, and the response of this system to bone damage and materials.

Research paper thumbnail of The effect of thyrocalcitonin on calcium uptake by isolated bone cells

The effect of thyrocalcitonin on calcium uptake by isolated bone cells

PubMed, Mar 1, 1971

Research paper thumbnail of A modified form for blade type endosteal implant

A modified form for blade type endosteal implant

PubMed, 1972

Research paper thumbnail of Calcium Uptake by Cultured Bone Cells: The Role of Phosphate, Calcitonin and 1,25-(OH)2D3

Calcium Uptake by Cultured Bone Cells: The Role of Phosphate, Calcitonin and 1,25-(OH)2D3

Springer eBooks, 1976

Research paper thumbnail of Partial characterization of a specific high affinity binding macromolecule for 24R,25 dihydroxyvitamin D3 in differentiating skeletal mesenchyme

Biochemical and Biophysical Research Communications, 1982

Research paper thumbnail of 873 Spontaneous Transformation of Cultured Skin Fibroblasts Into Osteoblasts in a Case of Dysplastic Intra Dermal Calcification

Pediatric Research, Apr 1, 1978

Research paper thumbnail of Heterologous tooth transplant. Eight years follow-up

Heterologous tooth transplant. Eight years follow-up

PubMed, 1986

Research paper thumbnail of Topical application of thyrocalcitonin on the exposed pulp of the rat molar

Topical application of thyrocalcitonin on the exposed pulp of the rat molar

PubMed, Sep 1, 1981

Research paper thumbnail of Biological Aspects

Biological Aspects

Springer eBooks, 2019

Research paper thumbnail of Tissue culture of bone cells: mineral transport, calcification and hormonal effects

Tissue culture of bone cells: mineral transport, calcification and hormonal effects

PubMed, Feb 1, 1976

Research paper thumbnail of Effect of dihydrotachysterol on bone induction in ovariectomized rats

Bone and Mineral, Mar 1, 1989

we have demonstrated via marr"v atromat CEH Eld1"TC6 and the aaeoinductive rclpanm IO deminera. l... more we have demonstrated via marr"v atromat CEH Eld1"TC6 and the aaeoinductive rclpanm IO deminera. lized bone @tr (DSMj i.*, ,ilU cvr,icai bu.. &r&f in ,hc ovrrirtamized (CWX) ,a.t MueeLspostop) ispdmarilydue ,oimpaircdo~,eopraS~ni,orcellproliiera,ion. andthatdihydmtachy~tsrol (DHT) marmen, can be protective. In cultured marrow stromal cells from DVX rats. short-lem~ DHT-R, uaggera,ed the already subnormal pattern 01 ma,,ow stromal cell proliferation. However. in DBM grafts. DHT lrealmen, benefited the time-course of mcxnchymal cell DNAsyntheris asmsasurcd by triliated Ihymid,ne incorporation z!+ weagenic cell ma,u,a,irn as measured by alkaline phmphatase mncen,m,ian. and established a r"SSes,ive ,rcnd D ward normalizado" of bone forma,io~minera,iza,ion (24 h %a incorporation). The dam from this animal model infer that DHT could moderate the bone (OS normally ~fcn in ovarieclomized mavia an activation of the arfeapmgenitorcell population. Key wwdm Osreoinduaion; Bone morphogenetie protein; Deminerakzed bone; Dihydmrachywsrol; Alkaline phospharau; Ovaris,amy and cortical bone mass [I ,32.39].

Research paper thumbnail of Evidence for 24,25-dihydroxycholecalciferol receptors in long bones of newborn rats

Biochemical Journal, Apr 15, 1982

Several reports have appeared that suggest that 24,25-dihydroxycholecalciferol has a possible bio... more Several reports have appeared that suggest that 24,25-dihydroxycholecalciferol has a possible biological role in bone formation. We have utilized competition studies, saturation analysis, sucrose-density-gradient sedimentation and DEAE-cellulose chromatography to demonstrate that long bones of vitamin D-depleted newborn rats contain cytoplasmic and possibly nuclear receptors that bind 24,25-dihydroxycholecalciferol with specificity and high affinity (Kd = 1.79nM). Sucrose-densitygradient analysis of the cytoplasmic 24,25-dihydroxycholecalciferol-binding component showed a single binding macromolecule for 24,25-dihydroxycholecalciferol with a sedimentation coefficient of 3.1 S. DEAE-cellulose chromatography showed a [3H]24,25-dihydroxycholecalciferol-macromolecular complex that binds to DEAEcellulose and elutes between 0.15 and 0.21 M-KCI. The finding of 24,25-dihydroxycholecalciferol receptors in long bones of newborn rats suggests a possible involvement of 24,25-dihydroxycholecalciferol in the metabolism of developing skeletal tissues.

Research paper thumbnail of Biochemical effect of mechanical stress on cultured bone cells

Calcified tissue research, Dec 1, 1976

The mode of transmission of mechanical stimuli into biochemical signals has intrigued research wo... more The mode of transmission of mechanical stimuli into biochemical signals has intrigued research workers for many years. Piatier-Piketty et al. [7], Rodan et al. [8], Davidowitch et al. [2] and Hong et al. [4] have described changes in cell membranes and in the amount of intracellular cyclic nucleotides [2, 7, 8] and of prostaglandins [4] produced by different forms of mechanical stress. The present paper reports evidence for the existence of a stimulus-receptor system in which a distortion of the cell membrane by mechanical perturbations initiates specific biochemical changes inside the cell. We have previously reported that cultured cells derived from the embryonal rat periosteum proliferate, differentiate, are metabolically active and retain their specific response to hormonal treatment. By using this system we were able to expose the cells to mechanical stress.

Research paper thumbnail of The cephalization index: A screening device for brain maturity and vulnerability in normal and intrauterine growth retarded newborns

Brain & Development, 1985

Predictive estimates of future neurological maldevelopment as a result of vascular induced intrau... more Predictive estimates of future neurological maldevelopment as a result of vascular induced intrauterine injury are based on the assumption that the body is more affected than the brain resulting in asymmetrical intrauterine growth retarded (IUCR) newborns. The higher the brain:body ratio, the more severe the IUCR process and the greater the risk for the brain to be affected. This prompted us to study in human newborns, a cephalization index based on the ratio of head circumference to body weight to express the degree of brain maturity and possible vulnerability in relation to gestational age. The newborn cephalization index was correlated with neurodevelopment. A trend could be delineated; in the later gestational age, the higher the cephalization index reflecting a greater degree of brain vulnerability, the more severe the clinical pathology; especially the likelihood of cerebral palsy and severe psychomotor retardation. The cephalization index may serve as an additional screening device for high risk intrauterine growth retarded newborns.

In the present study we have investigated the possibility of the use of a ‘“‘cephalization index” (Cl) based on the ratio of head cir- cumference to body weight defined in weeks of immaturity and expressing possible brain vulnerability. The Cl is suggested to serve as an additional screening device to predict normal or adverse brain development in high risk SFD newborns.

Research paper thumbnail of Brain: Body ratio and conceptional age in vascular-induced intrauterine growth retarded rabbits

Brain & Development, 1985

Vascular induced intrauterine growth retardation (IUGR) was achieved by total ligation of approxi... more Vascular induced intrauterine growth retardation (IUGR) was achieved by total ligation of approximately 30% of the placental vessels to half the fetuses in the last third of gestation in pregnant rabbits. A correlation between brain weight, body weight and head circumference was established in fetuses and rabbit pups in the perinatal period. The brain: body ratio in restricted IUGR animals was significantly higher than their homologous normal controls. A cephalization index based on the brain:body ratio is proposed to assess adverse effects on brain maturity in the presence of IUGR induced by placental insufficiency. Harel S, Yavin E, Tomer A, Barak Y, Binderman 1. Brain: body ratio and conceptional age in vascular-induced intrauterine growth retarded rabbits. Accumulation of clinical data in humans has strongly implicated an association between prolonged placental insufficiency from various causes and intrauterine growth retardation (IUGR) resulting in small-for-gestational age (SGA) newborns. Prenatal undernutrition has been extensively studied. Maternal dietary restriction has been shown to affect the brain growth of rats, guinea pigs and humans [I, 2]. Another approach to study this aspect of the problem in animals is to induce IUGR by uterine artery or placental vascular ligation and embolization. The classic work in this field was done by Wigglesworth [3] who experi-From the

Research paper thumbnail of Role of arachidonate in load transduction in bone cells

Role of arachidonate in load transduction in bone cells

Cambridge University Press eBooks, Apr 28, 1994

Research paper thumbnail of Effect of Fluoride on Hypervitaminosis D in Rats

Effect of Fluoride on Hypervitaminosis D in Rats

Journal of Dental Research, May 1, 1966

SYNOPSIS IN INTERLINGUA LE EFFECTO DE FLUORURO SUPER HYPERVITAMINOSIS D IN RATTOS.-Esseva usate r... more SYNOPSIS IN INTERLINGUA LE EFFECTO DE FLUORURO SUPER HYPERVITAMINOSIS D IN RATTOS.-Esseva usate rattos recipiente 0,2 mg de fluoruro per die (i.e., approximativemente le ingestion physiologic pro le ratto), rattos recipiente doses toxic de vitamina D, rattos recipiente vitamina D e fluoruro simultaneemente, e rattos de controlo. Esseva effectuate determinationes de calcium in le sero e le urina e del gravitate specific e del contento de cinere in le femure si ben como su contento de calcium. Le resultatos revelava que fluoruro age contra le syndrome de hypervitaminosis D per reducer usque ad valores quasi normal le concentrationes de calcium in le sero e le urina e etiam que illo preveni le resorption de osso. Es suggestionate que in hypervitaminosis D, le presentia de un augmentate concentration de iones de fluoruro in le liquidos del tissus induce un precipitation de fluoro-apatite.

Research paper thumbnail of The importance of inorganic phosphate for cartilage mineralization in culture

The importance of inorganic phosphate for cartilage mineralization in culture

Bone and Mineral, Apr 1, 1992

Research paper thumbnail of Induction of Osteogenesis by Free Periosteum Autografts

Journal of Dental Research, Sep 1, 1977

There have been numerous attempts to induce bone formation by dead bone, living bone or bone marr... more There have been numerous attempts to induce bone formation by dead bone, living bone or bone marrow tissue. Some of the positive results were interpreted as favoring the hypothesis that transformation of the surrounding connective tissue into bone is induced by osteogenic substances (AmITANI et al, GANN 66:327, 1975