Ivan Tulic - Academia.edu (original) (raw)
Papers by Ivan Tulic
Biological Trace Element Research, 2018
The aim of this study was to investigate the association of trace element and toxic metal concent... more The aim of this study was to investigate the association of trace element and toxic metal concentrations in blood and the outcome of in vitro fertilization (IVF). The study included 104 consecutive patients that underwent assisted reproductive technology (ART) procedures. The following parameters were determined: cadmium (Cd), mercury (Hg), arsenic (As), and lead (Pb); and copper (Cu), zinc (Zn), selenium (Se), and magnesium (Mg). Serum samples were obtained before commencing stimulation. Patients with smoking habit had significantly higher Pb concentrations (P = 0.022), as well as higher concentrations of As and Hg but not significantly. All subjects were divided into groups of pregnant and nonpregnant patients. Pregnant patients had lower mean values of Mg (P = 0.009), As (P < 0.05), and Pb (P = 0.034), compared to nonpregnant, and a significant correlation between pregnancy outcome and concentrations of Mg, Cd, and Pb was found. Women who had had delivered had lower Mg (P = 0.009) and Cd (P = 0.014) concentrations. There was a significant correlation of the negative outcome of IVF procedure with higher concentrations of Pb (P = 0.046) and Cd (P = 0.012). In conclusion, our results suggest that there is a difference in Mg, Pb, and Cd concentrations between pregnant and nonpregnant women. There was no association between toxic metals and number and quality of oocytes and embryos, while there was with fertilization rate. Concerning trace elements, we did not find the correlation of trace elements with oocyte number and quality, nor with a number of fertilized oocytes, except for Cu. Patients who were pregnant had lower concentrations of Mg.
Pregnancy Hypertension, 2020
Background: Early pathological changes in white matter microstructure can be studied using the di... more Background: Early pathological changes in white matter microstructure can be studied using the diffusion tensor imaging (DTI). It is not only important to study these subtle pathological changes leading to cognitive decline, but also to ascertain how an intervention would impact the white matter microstructure and cognition in persons at-risk of dementia. Objectives: To study the impact of a multidomain lifestyle intervention on white matter and cognitive changes during the 2year Finnish Geriatric Intervention Study to prevent Cognitive Impairment and Disability (FINGER), a randomized controlled trial in at-risk older individuals (age 60-77 years) from the general population. Methods: This exploratory study consisted of a subsample of 60 FINGER participants. Participants were randomized to either a multidomain intervention (diet, exercise, cognitive training, and vascular risk management, n = 34) or control group (general health advice, n = 26). All underwent baseline and 2-year brain DTI. Changes in fractional anisotropy (FA), diffusivity along domain (F1) and non-domain (F2) diffusion orientations, mean diffusivity (MD), axial diffusivity (AxD), radial diffusivity (RD), and their correlations with cognitive changes during the 2-year multidomain intervention were analyzed. Results: FA decreased, and cognition improved more in the intervention group compared to the control group (p < 0.05), with no significant intergroup differences for changes in F1, F2, MD, AxD, or RD. The cognitive changes were significantly positively related to FA change, and negatively related to RD change in the control group, but not in the intervention group. Conclusion: The 2-year multidomain FINGER intervention may modulate white matter microstructural alterations.
Human Reproduction, Oct 1, 1996
The purpose of this study was to explore whether cyclooxygenase products derived from endothelium... more The purpose of this study was to explore whether cyclooxygenase products derived from endothelium or vascular smooth muscle participate in the response of human uterine artery to prostaglandin F^. Experiments were performed using human uterine arterial rings. Prostaglandin F(0.4 • nM-1 uM) induced contraction of human uterine arteries with both intact and denuded endothelium with similar potency and efficacy (pD 2 values: 7.93 ± 0.01 and 8.07 ± 0.03 for vessels with and without endothelium respectively; maxima] response values: 89.1 ± 4.7% and 923 ± 3.8% for vessels with and without endothelium respectively). Indomethacin (10 uM) significantly suppressed the maximum effects of prostaglandin F^ and induced a shift towards the right of the prostaglandin Fĉ oncentration-response curves, regardless of the endothelial condition. On the other hand, in both types of preparations, OKY-046 (10 uM), an inhibitor of thromboxane synthesis, did not affect prostaglandin F^-induced contraction of human uterine arteries. It is concluded that in human uterine artery prostaglandin F 2a-induced contraction is mediated, at least in part, through constrictor prostanoid(s) of vascular smooth muscle origin that is not thromboxane A 2 .
Molecular Human Reproduction, 1998
European Journal of Pharmacology, 1994
The effect of L-arginine on isolated human uterine artery rings was investigated. L-Arginine, but... more The effect of L-arginine on isolated human uterine artery rings was investigated. L-Arginine, but not D-arginine, induced concentration-dependent relaxation. Removal of the endothelium enhanced the relaxant effects of L-arginine. Methylene blue and dexamethasone non-competitively inhibited L-arginine-induced relaxation, while NG-monomethyl-L-arginine competitively antagonized the response to L-arginine. Calmidazolium did not affect relaxation evoked by L-arginine. The dissociation constants obtained for L-arginine and NG-monomethyl-L-arginine in intact rings were not significantly different from those in endothelium-denuded rings. It is concluded that the relaxation induced by L-arginine in human uterine artery is mediated by non-endothelial nitric oxide production. We suggest that the NO synthase mediating the L-arginine-induced relaxation is an inducible type.
British Journal of Pharmacology, 1995
British Journal of Pharmacology, 1997
Srpski arhiv za celokupno lekarstvo
Correlation of oxidative stress markers and semen parameters with the outcome of in vitro fertili... more Correlation of oxidative stress markers and semen parameters with the outcome of in vitro fertilization Корелација маркера оксидативног стреса и параметара спермограма са исходом вантелесне оплодње SUMMARY Introduction/Objective The aim is assessing the effect of oxidative stress parameters on sperm count, motility, and morphology of spermatozoa, and the influence of different semen parameters on the outcome of in vitro fertilization (IVF) procedure-the number of fertilized oocytes, fertilization, and pregnancy rate of female partners. Method In 52 male patients superoxide dismutase (SOD), malondialdehyde (MDA) and sulfhydryl (-SH) groups were determined in serum, before beginning the IVF cycle. Semen samples were collected after 2-3 days of sexual abstinence. Results Patients were divided into two groups, the normozoospermic, and those with pathological sperm findings. The second group was divided into oligozoospermic, asthenozoospermic, and teratozoospermic groups. SOD activity was lower, while MDA and-SH groups, while not significantly, was higher in groups with sperm parameter disorders. Fertilization rate was significantly lower in the group of asthenozoospermia and teratozoospermia (p = 0.034), as well as delivery rate (p = 0.020). The group with oligozoospermia had significantly lower delivery rate (p = 0.013). Conclusion Our study found higher fertilization and delivery rate in men with normozoospermia. However, no significant correlation between OS markers and semen parameters was found.
Human Reproduction, 1996
The purpose of this study was to explore whether cyclooxygenase products derived from endothelium... more The purpose of this study was to explore whether cyclooxygenase products derived from endothelium or vascular smooth muscle participate in the response of human uterine artery to prostaglandin F^. Experiments were performed using human uterine arterial rings. Prostaglandin F(0.4 • nM-1 uM) induced contraction of human uterine arteries with both intact and denuded endothelium with similar potency and efficacy (pD 2 values: 7.93 ± 0.01 and 8.07 ± 0.03 for vessels with and without endothelium respectively; maxima] response values: 89.1 ± 4.7% and 923 ± 3.8% for vessels with and without endothelium respectively). Indomethacin (10 uM) significantly suppressed the maximum effects of prostaglandin F^ and induced a shift towards the right of the prostaglandin Fĉ oncentration-response curves, regardless of the endothelial condition. On the other hand, in both types of preparations, OKY-046 (10 uM), an inhibitor of thromboxane synthesis, did not affect prostaglandin F^-induced contraction of human uterine arteries. It is concluded that in human uterine artery prostaglandin F 2a-induced contraction is mediated, at least in part, through constrictor prostanoid(s) of vascular smooth muscle origin that is not thromboxane A 2 .
Journal of Medical Biochemistry, 2017
SummaryBackground: Our aim was to study the effect of GnRH agonist and antagonist protocols of ov... more SummaryBackground: Our aim was to study the effect of GnRH agonist and antagonist protocols of ovarian stimulation on oxidative stress parameters in serum and the influence of oxidative stress parameters change on the outcome of IVF cycles.Methods: This prospective study included 82 patients who un der went IVF procedures. We determined SOD, MDA and SH groups in serum. Serum samples were obtained between the second and fourth day of the cycle and on the day of HCG administration during ovarian stimulation.Results: Patients were divided into two groups depending on the protocol of stimulation. The mean total and mature oocytes number and number of fertilized oocytes were higher in GnRH agonist group. There was no significant difference in biochemical pregnancy, miscarriage and live -birth rate in both groups. Mean serum SOD was significantly lower, while mean serum MDA and SH groups were significantly higher after ovarian stimulation. Delivery rate was higher in patients without OS w...
Jugoslavenska ginekologija i perinatologija
An analysis was made of 75 pregnant patients who received medicamentous therapy for Graves' d... more An analysis was made of 75 pregnant patients who received medicamentous therapy for Graves' disease before the investigated pregnancy and 20 healthy pregnant women with normal pregnancies and term delivery. A severe form of hyperthyreosis was found in 35 examined persons. A separate analysis was made of the patients who had no previous therapy (17) and pregnant patients who were treated with antithyroid drugs (18), with the aim to investigate their effect on the course and outcome of pregnancy and the condition of the newborn infant. In all examined pregnant women the median values with standard deviations for TSH, T4 and T3 in each trimester of pregnancy, the dynamics of their trends, as well as the correlation of values in treated and untreated pregnant women were recorded. The analysis of the pregnancy course in patients with hyperthyreosis indicated a significantly higher incidence of gestational diabetes and EPH gestoses (p less than 0.001). A separate analysis indicated th...
Srpski arhiv za celokupno lekarstvo
In order to examine the thyroid gland function we analysed healthy pregnant women (20) with norma... more In order to examine the thyroid gland function we analysed healthy pregnant women (20) with normal pregnancy course and term delivery, who had normal endocrinological findings before the investigated pregnancy. In all patients TSH, T4 and T3 concentrations were radioimmunologically determined by trimester, median values with standard deviations were analysed in order to establish normal values during gravidity, as well as the possible changes in relation to non-gravid condition. We determined that T4 (29.3%) and T3 (23.5%) concentrations significantly increased and that TSH insignificantly decreased (12.1%) in the first trimester of pregnancy. In healthy pregnant women during the advance of gestation there was a significant increase of T4 and T3, which was significantly higher in the following than in the previous trimester. TSH concentrations during pregnancy gradually decreased but the decrease during the whole course of pregnancy, and in separate trimesters, was not found to be s...
Vojnosanitetski pregled, 2006
Klinički centar Srbije, Institut za ginekologiju i akušerstvo, Beograd Apstrakt Uvod/Cilj. Tibolo... more Klinički centar Srbije, Institut za ginekologiju i akušerstvo, Beograd Apstrakt Uvod/Cilj. Tibolon je preparat koji pripada grupi steroidnih supstancija. Efekti primene tibolona su posledica aktivnosti njegovih metabolita pri čemu njihova hormonska aktivnost zavisi od vrste tkiva u kome se stvaraju. Cilj rada je bio ispitivanje efekta primene tibolona na faktore rizika za nastanak kardiovaskularnih oboljenja kod žena u postmenopauzi. Metode. U prospektivnu studiju je uključeno 94 ispitanica koje su imale koncentraciju 17ß-estradiola < od 50 pg/ml i koje su bile u menopauzi bar jednu godinu. Od ukupnog broja, 63 ispitanice koje su prihvatile da primaju tibolon u dozi od 2,5mg na dan, činile su ispitivanu tibolon (T) grupu, dok je 31 ispitanica odbila da koristi supstitucionu terapiju i one su činile kontrolnu (K) grupu. Merene su koncentracije lipida (ukupni holesterol, LDL holesterol, HDL holesterol, trigliceridi), antitrombina III, fibrinogena i C-reaktivnog proteina (CRP) pre i posle terapije tibolonom koja je trajala šest meseci, a zatim je ispitivana statistička značajnost razlike u vrednostima posmatranih koncentracija. Ispitivane su i promene u vrednostima ispitivanih koncentracija u kontrolnoj grupi žena. Rezultati. Kod 31 ispitanice u postmenopauzi koje su činile kontrolnu grupu, nije došlo do značajnije promene vrednosti definisanih parametara u odnosu na njihove početne vrednosti, tokom posmatranog perioda. Kod ispitanica kod kojih je primenjen tibolon došlo je do značajnih promena u vrednostima posmatranih parametara (p < 0,001) tokom perioda od šest meseci, kao što je smanjenje vrednosti ukupnog holesterola za 17,8%, smanjenje vrednosti HDL holesterola za 27%, smanjenje vrednosti LDL holesterola za 4% (bez statističke značajnosti p > 0,05) uz smanjenje vrednosti triglicerida za 35%. Nije uočena statistička značajnost u promeni koncentracije antitrombina III, fibrinogena i CRP nakon primene tibolona. Zaključak. Primena tibolona dovodi do smanjenja vrednosti ukupnog holesterola, triglicerida, HDL holesterola, ne smanjujući značajno vrednost LDL holesterola. Takođe primena tibolona ne utiče značajno na promene koncentracije fibrinogena, antitrombina III i CRP. Broj serumskih parametara merenih u ovoj studiji je ograničen, zbog čega se može diskutovati samo o metabolizmu lipida, dok definitivni zaključak o riziku za nastanak kardiovaskularnih oboljenja tokom primene tibolona zahteva dalja klinička istraživanja.
Vojnosanitetski pregled, 2006
Klinički centar Srbije, Institut za ginekologiju i akušerstvo, Beograd Apstrakt Uvod/Cilj. Primen... more Klinički centar Srbije, Institut za ginekologiju i akušerstvo, Beograd Apstrakt Uvod/Cilj. Primena medikamentnog prekida trudnoće odobrena je u našoj zemlji od septembra 2001. godine. Cilj našeg istraživanja je procena efikasnosti primene ove metode, učestalosti pojave neželjenih efekata, kao i prihvatljivosti primene ove metode kao načina prekida rane trudnoće, primenom mifepristona oralno (600 mg) i 48 sati kasnije misoprostola, oralno ili vaginalno u različitim dozama (400, 600 ili 800 μg), u našoj populaciji. Metode. Ispitivanjem je obuhvaćeno 235 žena sa trudnoćom do 49. dana amenoreje koje su na osnovu načina primene i primenjene doze misoprostola podeljene u četiri grupe (I grupa 400 μg, II 600 μg, III 800 μg oralno i IV grupa 800 μg podeljenih oralno i vaginalno). Metoda je označena kao uspešna u slučaju kompletnog abortusa. Pored uspešnosti metode, praćena je i učestalost pojave neželjenih efekata i komplikacija. Rezultati. Efikasnost metoda izražena kao kompletna ekspulzija produkata začeća bez potrebe za instrumentalnom intervencijom bila je u I grupi 50%, 89,48% u II, 75% u III i 92,11% u IV grupi, što četvrti protokol čini najuspešnijim (t 1:4 = 7,005; t 2:4 = 0,3872, t 3:4 = 2,9784, p < 0,01). Učestalost pojave neželjenih efekata (abdominalni bol, povraćanje, vaginalno krvarenje, groznica) bila je niska i javljale su se kod viših oralnih doza misoprostola (600 i 800 μg). U samo jednom slučaju je bila zbog profuzne hemoragije neophodna hitna hemostatska kiretaža i primena dve transfuzije krvi. Nisu zabeleženi slučajevi neprekinutih trudnoća. Zaključak. Naša studija je pokazala da najefikasniji protokol za medikamentni prekid trudnoće, istovremeno sa najmanje neželjenih efekata, obuhvata oralnu primenu 600 mg mifepristona i kombinovanu oralnu i vaginalnu primenu 800 μg misoprostola. Nadalje, ona sugeriše da bi ovaj način prekida trudnoće mogao postići veći udeo u ukupnom broju prekida rane trudnoće.
Reproduction, Fertility and Development, 2014
Developmental dysfunction in embryos, such as a lethal level of fragmentation, is assumed to be m... more Developmental dysfunction in embryos, such as a lethal level of fragmentation, is assumed to be mitochondrial in origin. This study investigated the molecular basis of mitochondrial impairment in embryo fragmentation. Transcription patterns of factors that determine mitochondrial functionality: (i) components of the oxidative phosphorylation (OXPHOS) - complex I, cytochrome b, complex IV and ATP synthase; (ii) mitochondrial membrane potential (MMP); (iii) mitochondrial DNA (mtDNA) content and (iv) proteins involved in mitochondrial dynamics, mitofusin 1 (Mfn1) and dynamin related protein 1 (Drp1) were examined in six-cells Day 3 non-fragmented (control), low-fragmented (LF) and high-fragmented (HF) human embryos. Gene expression of mitochondria-encoded components of complex I and IV, cytochrome b and mtDNA were increased in HF embryos compared with control and LF embryos. In LF embryos, expression of these molecules was decreased compared with control and HF embryos. Both classes of fragmented embryos had decreased MMP compared with control. LF embryos had increased gene expression of Mfn1 accompanied by decreased expression of Drp1, while HF embryos had decreased Mfn1 expression but increased Drp1 expression. The study revealed that each improper transcriptional (in)activation of mitochondria-encoded components of the OXPHOS during early in vitro embryo development is associated with a decrease in MMP and with embryo fragmentation. The results also showed the importance of mitochondrial dynamics in fragmentation, at least in the extent of this process.
Mechanisms of Ageing and Development, 2013
Fertility and Sterility, 2011
To report a case of successful pregnancy and delivery after IVF and ET in a patient with Swyer sy... more To report a case of successful pregnancy and delivery after IVF and ET in a patient with Swyer syndrome. Case report. Unit of Assisted Reproduction, Gynecology and Obstetrics Institute, University of Belgrade. A 30-year-old patient with 46,XY gonadal dysgenesis. Chromosomal analysis, diagnostic laparoscopy, IVF using donor oocytes, ET, and cesarean delivery. Successful pregnancy and live birth. Successful treatment, pregnancy, and delivery. A patient with 46,XY gonadal dysgenesis in a donor oocyte program, can maintain a normal pregnancy and delivery.
Jugoslovenska medicinska biohemija, 2004
... Zorica [umarac1, Violeta Dopsaj1, Ivan Tuli}2, Marijana Dajak1, Dragana Vukosavljevi}1, Sne`a... more ... Zorica [umarac1, Violeta Dopsaj1, Ivan Tuli}2, Marijana Dajak1, Dragana Vukosavljevi}1, Sne`ana Jovi~i}1, Nada Majki}-Singh1 ... 2. Healy DL, Polson DW, MacLachan V, Clarkson P. Ran-domized study Buserelin-hMG treatment in routine IVF patients. ...
Biological Trace Element Research, 2018
The aim of this study was to investigate the association of trace element and toxic metal concent... more The aim of this study was to investigate the association of trace element and toxic metal concentrations in blood and the outcome of in vitro fertilization (IVF). The study included 104 consecutive patients that underwent assisted reproductive technology (ART) procedures. The following parameters were determined: cadmium (Cd), mercury (Hg), arsenic (As), and lead (Pb); and copper (Cu), zinc (Zn), selenium (Se), and magnesium (Mg). Serum samples were obtained before commencing stimulation. Patients with smoking habit had significantly higher Pb concentrations (P = 0.022), as well as higher concentrations of As and Hg but not significantly. All subjects were divided into groups of pregnant and nonpregnant patients. Pregnant patients had lower mean values of Mg (P = 0.009), As (P < 0.05), and Pb (P = 0.034), compared to nonpregnant, and a significant correlation between pregnancy outcome and concentrations of Mg, Cd, and Pb was found. Women who had had delivered had lower Mg (P = 0.009) and Cd (P = 0.014) concentrations. There was a significant correlation of the negative outcome of IVF procedure with higher concentrations of Pb (P = 0.046) and Cd (P = 0.012). In conclusion, our results suggest that there is a difference in Mg, Pb, and Cd concentrations between pregnant and nonpregnant women. There was no association between toxic metals and number and quality of oocytes and embryos, while there was with fertilization rate. Concerning trace elements, we did not find the correlation of trace elements with oocyte number and quality, nor with a number of fertilized oocytes, except for Cu. Patients who were pregnant had lower concentrations of Mg.
Pregnancy Hypertension, 2020
Background: Early pathological changes in white matter microstructure can be studied using the di... more Background: Early pathological changes in white matter microstructure can be studied using the diffusion tensor imaging (DTI). It is not only important to study these subtle pathological changes leading to cognitive decline, but also to ascertain how an intervention would impact the white matter microstructure and cognition in persons at-risk of dementia. Objectives: To study the impact of a multidomain lifestyle intervention on white matter and cognitive changes during the 2year Finnish Geriatric Intervention Study to prevent Cognitive Impairment and Disability (FINGER), a randomized controlled trial in at-risk older individuals (age 60-77 years) from the general population. Methods: This exploratory study consisted of a subsample of 60 FINGER participants. Participants were randomized to either a multidomain intervention (diet, exercise, cognitive training, and vascular risk management, n = 34) or control group (general health advice, n = 26). All underwent baseline and 2-year brain DTI. Changes in fractional anisotropy (FA), diffusivity along domain (F1) and non-domain (F2) diffusion orientations, mean diffusivity (MD), axial diffusivity (AxD), radial diffusivity (RD), and their correlations with cognitive changes during the 2-year multidomain intervention were analyzed. Results: FA decreased, and cognition improved more in the intervention group compared to the control group (p < 0.05), with no significant intergroup differences for changes in F1, F2, MD, AxD, or RD. The cognitive changes were significantly positively related to FA change, and negatively related to RD change in the control group, but not in the intervention group. Conclusion: The 2-year multidomain FINGER intervention may modulate white matter microstructural alterations.
Human Reproduction, Oct 1, 1996
The purpose of this study was to explore whether cyclooxygenase products derived from endothelium... more The purpose of this study was to explore whether cyclooxygenase products derived from endothelium or vascular smooth muscle participate in the response of human uterine artery to prostaglandin F^. Experiments were performed using human uterine arterial rings. Prostaglandin F(0.4 • nM-1 uM) induced contraction of human uterine arteries with both intact and denuded endothelium with similar potency and efficacy (pD 2 values: 7.93 ± 0.01 and 8.07 ± 0.03 for vessels with and without endothelium respectively; maxima] response values: 89.1 ± 4.7% and 923 ± 3.8% for vessels with and without endothelium respectively). Indomethacin (10 uM) significantly suppressed the maximum effects of prostaglandin F^ and induced a shift towards the right of the prostaglandin Fĉ oncentration-response curves, regardless of the endothelial condition. On the other hand, in both types of preparations, OKY-046 (10 uM), an inhibitor of thromboxane synthesis, did not affect prostaglandin F^-induced contraction of human uterine arteries. It is concluded that in human uterine artery prostaglandin F 2a-induced contraction is mediated, at least in part, through constrictor prostanoid(s) of vascular smooth muscle origin that is not thromboxane A 2 .
Molecular Human Reproduction, 1998
European Journal of Pharmacology, 1994
The effect of L-arginine on isolated human uterine artery rings was investigated. L-Arginine, but... more The effect of L-arginine on isolated human uterine artery rings was investigated. L-Arginine, but not D-arginine, induced concentration-dependent relaxation. Removal of the endothelium enhanced the relaxant effects of L-arginine. Methylene blue and dexamethasone non-competitively inhibited L-arginine-induced relaxation, while NG-monomethyl-L-arginine competitively antagonized the response to L-arginine. Calmidazolium did not affect relaxation evoked by L-arginine. The dissociation constants obtained for L-arginine and NG-monomethyl-L-arginine in intact rings were not significantly different from those in endothelium-denuded rings. It is concluded that the relaxation induced by L-arginine in human uterine artery is mediated by non-endothelial nitric oxide production. We suggest that the NO synthase mediating the L-arginine-induced relaxation is an inducible type.
British Journal of Pharmacology, 1995
British Journal of Pharmacology, 1997
Srpski arhiv za celokupno lekarstvo
Correlation of oxidative stress markers and semen parameters with the outcome of in vitro fertili... more Correlation of oxidative stress markers and semen parameters with the outcome of in vitro fertilization Корелација маркера оксидативног стреса и параметара спермограма са исходом вантелесне оплодње SUMMARY Introduction/Objective The aim is assessing the effect of oxidative stress parameters on sperm count, motility, and morphology of spermatozoa, and the influence of different semen parameters on the outcome of in vitro fertilization (IVF) procedure-the number of fertilized oocytes, fertilization, and pregnancy rate of female partners. Method In 52 male patients superoxide dismutase (SOD), malondialdehyde (MDA) and sulfhydryl (-SH) groups were determined in serum, before beginning the IVF cycle. Semen samples were collected after 2-3 days of sexual abstinence. Results Patients were divided into two groups, the normozoospermic, and those with pathological sperm findings. The second group was divided into oligozoospermic, asthenozoospermic, and teratozoospermic groups. SOD activity was lower, while MDA and-SH groups, while not significantly, was higher in groups with sperm parameter disorders. Fertilization rate was significantly lower in the group of asthenozoospermia and teratozoospermia (p = 0.034), as well as delivery rate (p = 0.020). The group with oligozoospermia had significantly lower delivery rate (p = 0.013). Conclusion Our study found higher fertilization and delivery rate in men with normozoospermia. However, no significant correlation between OS markers and semen parameters was found.
Human Reproduction, 1996
The purpose of this study was to explore whether cyclooxygenase products derived from endothelium... more The purpose of this study was to explore whether cyclooxygenase products derived from endothelium or vascular smooth muscle participate in the response of human uterine artery to prostaglandin F^. Experiments were performed using human uterine arterial rings. Prostaglandin F(0.4 • nM-1 uM) induced contraction of human uterine arteries with both intact and denuded endothelium with similar potency and efficacy (pD 2 values: 7.93 ± 0.01 and 8.07 ± 0.03 for vessels with and without endothelium respectively; maxima] response values: 89.1 ± 4.7% and 923 ± 3.8% for vessels with and without endothelium respectively). Indomethacin (10 uM) significantly suppressed the maximum effects of prostaglandin F^ and induced a shift towards the right of the prostaglandin Fĉ oncentration-response curves, regardless of the endothelial condition. On the other hand, in both types of preparations, OKY-046 (10 uM), an inhibitor of thromboxane synthesis, did not affect prostaglandin F^-induced contraction of human uterine arteries. It is concluded that in human uterine artery prostaglandin F 2a-induced contraction is mediated, at least in part, through constrictor prostanoid(s) of vascular smooth muscle origin that is not thromboxane A 2 .
Journal of Medical Biochemistry, 2017
SummaryBackground: Our aim was to study the effect of GnRH agonist and antagonist protocols of ov... more SummaryBackground: Our aim was to study the effect of GnRH agonist and antagonist protocols of ovarian stimulation on oxidative stress parameters in serum and the influence of oxidative stress parameters change on the outcome of IVF cycles.Methods: This prospective study included 82 patients who un der went IVF procedures. We determined SOD, MDA and SH groups in serum. Serum samples were obtained between the second and fourth day of the cycle and on the day of HCG administration during ovarian stimulation.Results: Patients were divided into two groups depending on the protocol of stimulation. The mean total and mature oocytes number and number of fertilized oocytes were higher in GnRH agonist group. There was no significant difference in biochemical pregnancy, miscarriage and live -birth rate in both groups. Mean serum SOD was significantly lower, while mean serum MDA and SH groups were significantly higher after ovarian stimulation. Delivery rate was higher in patients without OS w...
Jugoslavenska ginekologija i perinatologija
An analysis was made of 75 pregnant patients who received medicamentous therapy for Graves' d... more An analysis was made of 75 pregnant patients who received medicamentous therapy for Graves' disease before the investigated pregnancy and 20 healthy pregnant women with normal pregnancies and term delivery. A severe form of hyperthyreosis was found in 35 examined persons. A separate analysis was made of the patients who had no previous therapy (17) and pregnant patients who were treated with antithyroid drugs (18), with the aim to investigate their effect on the course and outcome of pregnancy and the condition of the newborn infant. In all examined pregnant women the median values with standard deviations for TSH, T4 and T3 in each trimester of pregnancy, the dynamics of their trends, as well as the correlation of values in treated and untreated pregnant women were recorded. The analysis of the pregnancy course in patients with hyperthyreosis indicated a significantly higher incidence of gestational diabetes and EPH gestoses (p less than 0.001). A separate analysis indicated th...
Srpski arhiv za celokupno lekarstvo
In order to examine the thyroid gland function we analysed healthy pregnant women (20) with norma... more In order to examine the thyroid gland function we analysed healthy pregnant women (20) with normal pregnancy course and term delivery, who had normal endocrinological findings before the investigated pregnancy. In all patients TSH, T4 and T3 concentrations were radioimmunologically determined by trimester, median values with standard deviations were analysed in order to establish normal values during gravidity, as well as the possible changes in relation to non-gravid condition. We determined that T4 (29.3%) and T3 (23.5%) concentrations significantly increased and that TSH insignificantly decreased (12.1%) in the first trimester of pregnancy. In healthy pregnant women during the advance of gestation there was a significant increase of T4 and T3, which was significantly higher in the following than in the previous trimester. TSH concentrations during pregnancy gradually decreased but the decrease during the whole course of pregnancy, and in separate trimesters, was not found to be s...
Vojnosanitetski pregled, 2006
Klinički centar Srbije, Institut za ginekologiju i akušerstvo, Beograd Apstrakt Uvod/Cilj. Tibolo... more Klinički centar Srbije, Institut za ginekologiju i akušerstvo, Beograd Apstrakt Uvod/Cilj. Tibolon je preparat koji pripada grupi steroidnih supstancija. Efekti primene tibolona su posledica aktivnosti njegovih metabolita pri čemu njihova hormonska aktivnost zavisi od vrste tkiva u kome se stvaraju. Cilj rada je bio ispitivanje efekta primene tibolona na faktore rizika za nastanak kardiovaskularnih oboljenja kod žena u postmenopauzi. Metode. U prospektivnu studiju je uključeno 94 ispitanica koje su imale koncentraciju 17ß-estradiola < od 50 pg/ml i koje su bile u menopauzi bar jednu godinu. Od ukupnog broja, 63 ispitanice koje su prihvatile da primaju tibolon u dozi od 2,5mg na dan, činile su ispitivanu tibolon (T) grupu, dok je 31 ispitanica odbila da koristi supstitucionu terapiju i one su činile kontrolnu (K) grupu. Merene su koncentracije lipida (ukupni holesterol, LDL holesterol, HDL holesterol, trigliceridi), antitrombina III, fibrinogena i C-reaktivnog proteina (CRP) pre i posle terapije tibolonom koja je trajala šest meseci, a zatim je ispitivana statistička značajnost razlike u vrednostima posmatranih koncentracija. Ispitivane su i promene u vrednostima ispitivanih koncentracija u kontrolnoj grupi žena. Rezultati. Kod 31 ispitanice u postmenopauzi koje su činile kontrolnu grupu, nije došlo do značajnije promene vrednosti definisanih parametara u odnosu na njihove početne vrednosti, tokom posmatranog perioda. Kod ispitanica kod kojih je primenjen tibolon došlo je do značajnih promena u vrednostima posmatranih parametara (p < 0,001) tokom perioda od šest meseci, kao što je smanjenje vrednosti ukupnog holesterola za 17,8%, smanjenje vrednosti HDL holesterola za 27%, smanjenje vrednosti LDL holesterola za 4% (bez statističke značajnosti p > 0,05) uz smanjenje vrednosti triglicerida za 35%. Nije uočena statistička značajnost u promeni koncentracije antitrombina III, fibrinogena i CRP nakon primene tibolona. Zaključak. Primena tibolona dovodi do smanjenja vrednosti ukupnog holesterola, triglicerida, HDL holesterola, ne smanjujući značajno vrednost LDL holesterola. Takođe primena tibolona ne utiče značajno na promene koncentracije fibrinogena, antitrombina III i CRP. Broj serumskih parametara merenih u ovoj studiji je ograničen, zbog čega se može diskutovati samo o metabolizmu lipida, dok definitivni zaključak o riziku za nastanak kardiovaskularnih oboljenja tokom primene tibolona zahteva dalja klinička istraživanja.
Vojnosanitetski pregled, 2006
Klinički centar Srbije, Institut za ginekologiju i akušerstvo, Beograd Apstrakt Uvod/Cilj. Primen... more Klinički centar Srbije, Institut za ginekologiju i akušerstvo, Beograd Apstrakt Uvod/Cilj. Primena medikamentnog prekida trudnoće odobrena je u našoj zemlji od septembra 2001. godine. Cilj našeg istraživanja je procena efikasnosti primene ove metode, učestalosti pojave neželjenih efekata, kao i prihvatljivosti primene ove metode kao načina prekida rane trudnoće, primenom mifepristona oralno (600 mg) i 48 sati kasnije misoprostola, oralno ili vaginalno u različitim dozama (400, 600 ili 800 μg), u našoj populaciji. Metode. Ispitivanjem je obuhvaćeno 235 žena sa trudnoćom do 49. dana amenoreje koje su na osnovu načina primene i primenjene doze misoprostola podeljene u četiri grupe (I grupa 400 μg, II 600 μg, III 800 μg oralno i IV grupa 800 μg podeljenih oralno i vaginalno). Metoda je označena kao uspešna u slučaju kompletnog abortusa. Pored uspešnosti metode, praćena je i učestalost pojave neželjenih efekata i komplikacija. Rezultati. Efikasnost metoda izražena kao kompletna ekspulzija produkata začeća bez potrebe za instrumentalnom intervencijom bila je u I grupi 50%, 89,48% u II, 75% u III i 92,11% u IV grupi, što četvrti protokol čini najuspešnijim (t 1:4 = 7,005; t 2:4 = 0,3872, t 3:4 = 2,9784, p < 0,01). Učestalost pojave neželjenih efekata (abdominalni bol, povraćanje, vaginalno krvarenje, groznica) bila je niska i javljale su se kod viših oralnih doza misoprostola (600 i 800 μg). U samo jednom slučaju je bila zbog profuzne hemoragije neophodna hitna hemostatska kiretaža i primena dve transfuzije krvi. Nisu zabeleženi slučajevi neprekinutih trudnoća. Zaključak. Naša studija je pokazala da najefikasniji protokol za medikamentni prekid trudnoće, istovremeno sa najmanje neželjenih efekata, obuhvata oralnu primenu 600 mg mifepristona i kombinovanu oralnu i vaginalnu primenu 800 μg misoprostola. Nadalje, ona sugeriše da bi ovaj način prekida trudnoće mogao postići veći udeo u ukupnom broju prekida rane trudnoće.
Reproduction, Fertility and Development, 2014
Developmental dysfunction in embryos, such as a lethal level of fragmentation, is assumed to be m... more Developmental dysfunction in embryos, such as a lethal level of fragmentation, is assumed to be mitochondrial in origin. This study investigated the molecular basis of mitochondrial impairment in embryo fragmentation. Transcription patterns of factors that determine mitochondrial functionality: (i) components of the oxidative phosphorylation (OXPHOS) - complex I, cytochrome b, complex IV and ATP synthase; (ii) mitochondrial membrane potential (MMP); (iii) mitochondrial DNA (mtDNA) content and (iv) proteins involved in mitochondrial dynamics, mitofusin 1 (Mfn1) and dynamin related protein 1 (Drp1) were examined in six-cells Day 3 non-fragmented (control), low-fragmented (LF) and high-fragmented (HF) human embryos. Gene expression of mitochondria-encoded components of complex I and IV, cytochrome b and mtDNA were increased in HF embryos compared with control and LF embryos. In LF embryos, expression of these molecules was decreased compared with control and HF embryos. Both classes of fragmented embryos had decreased MMP compared with control. LF embryos had increased gene expression of Mfn1 accompanied by decreased expression of Drp1, while HF embryos had decreased Mfn1 expression but increased Drp1 expression. The study revealed that each improper transcriptional (in)activation of mitochondria-encoded components of the OXPHOS during early in vitro embryo development is associated with a decrease in MMP and with embryo fragmentation. The results also showed the importance of mitochondrial dynamics in fragmentation, at least in the extent of this process.
Mechanisms of Ageing and Development, 2013
Fertility and Sterility, 2011
To report a case of successful pregnancy and delivery after IVF and ET in a patient with Swyer sy... more To report a case of successful pregnancy and delivery after IVF and ET in a patient with Swyer syndrome. Case report. Unit of Assisted Reproduction, Gynecology and Obstetrics Institute, University of Belgrade. A 30-year-old patient with 46,XY gonadal dysgenesis. Chromosomal analysis, diagnostic laparoscopy, IVF using donor oocytes, ET, and cesarean delivery. Successful pregnancy and live birth. Successful treatment, pregnancy, and delivery. A patient with 46,XY gonadal dysgenesis in a donor oocyte program, can maintain a normal pregnancy and delivery.
Jugoslovenska medicinska biohemija, 2004
... Zorica [umarac1, Violeta Dopsaj1, Ivan Tuli}2, Marijana Dajak1, Dragana Vukosavljevi}1, Sne`a... more ... Zorica [umarac1, Violeta Dopsaj1, Ivan Tuli}2, Marijana Dajak1, Dragana Vukosavljevi}1, Sne`ana Jovi~i}1, Nada Majki}-Singh1 ... 2. Healy DL, Polson DW, MacLachan V, Clarkson P. Ran-domized study Buserelin-hMG treatment in routine IVF patients. ...