Ivana Pipinić - Academia.edu (original) (raw)

Papers by Ivana Pipinić

International Journal of Immunogenetics, Oct 18, 2012

Filaggrin gene (FLG) null mutations are considered associated with atopic dermatitis. This study ... more Filaggrin gene (FLG) null mutations are considered associated with atopic dermatitis. This study was conducted to determine the prevalence of FLG null mutations R501X, 2282del4, R2447X and S3247X in the Croatian population and their role in the occurrence of allergic diseases including atopic dermatitis, allergic rhinitis, asthma and allergic contact dermatitis (ACD). Study enrolled 440 freshmen with defined allergic diseases by means of both present symptoms in International Study of Asthma and Allergies in Childhood questionnaire (relevant respiratory and/or skin symptoms) and markers of allergic sensitization (positive skin prick and/or patch test). FLG null mutations were successfully genotyped in 423 students of which 11 (2.6%) were carriers of FLG null mutation: 1/423 (0.2%) was heterozygous for R501X and 10/423 (2.4%) were heterozygous for 2282del4. No carriers of R2447X and S3247X mutations were identified. In wild-type FLG carriers (412 subjects), atopic dermatitis was present in 45 (11%), allergic rhinitis in 70 (17%) and allergic asthma in 29 (7%) students. Twenty-five of 393 (7%) patch-tested wild-type FLG carriers had ACD. Among 11 FLG null mutation carriers, four had one or more allergic diseases, and five had reported skin symptoms without defined allergic sensitization (positive skin prick test and/or patch test). FLG null mutations were not confirmed as a predictor of analysed allergic diseases, but were confirmed as an independent predictor of skin symptoms (OR 17.19, 95% CI 3.41-86.6, P < 0.001). Our results in general indicate a low frequency of FLG null mutations in the studied Croatian population supporting a theory of a latitude-dependent distribution of FGL null mutations in Europe, with a decreasing northsouth gradient of R501X and 2282del4 mutation frequency. The relation between FLG null mutations and skin disorders was confirmed.

6. hrvatski kongres dječje kirurgije s međunarodnim sudjelovanjem. Završni program i knjiga sažetaka., 2011

Covjek oduvijek živi u simbiozi s grinjama u privatnim, javnim i radnim unutarnjim prostorima. Sv... more Covjek oduvijek živi u simbiozi s grinjama u privatnim, javnim i radnim unutarnjim prostorima. Sve cesca pojava alergija u ljudi, ucinila je prasinske grinje nepoželjnim stanovnicima mnogih ljudskih stanista. S obzirom na sve veci broj oboljelih od alergijskih bolesti, te sveprisutnost i brojnost populacije grinja u Hrvatskoj, mjere za smanjenje broja grinja u stanovima, javnim (skole, vrtici, kina, kazalista i sl.) i radnim unutarnjim prostorima dobivaju sve vecu važnost, u cilju prevencije nastanka alergije na grinje, kao i nastanka simptoma alergijskih bolesti uvjetovanih grinjama. Drugi je, ne manje važan, aspekt suzbijanja grinja kao stetnika poljoprivrednih proizvoda. Osnovne metode za suzbijanje prasinskih grinja su mehanicko odstranjivanje, nepropusne barijere, smanjenje vlažnosti zraka i druge fizikalne metode (primjena hladnoce ili topline, ventilacije, aktivnog ugljena), te kemijske metode (upotreba akaricidnih sredstava). Od akaricida najcesce se upotrebljavaju benzil-be...

International Archives of Allergy and Immunology, 2016

Background: Polymorphisms of cytokine genes are an interesting focus for association studies invo... more Background: Polymorphisms of cytokine genes are an interesting focus for association studies involving atopic diseases due to their role in immune cell communications during inflammation. The aim of this study was to investigate associations of TNFα -308G>A, TNFα -238G>A, IL-1α -889C>T and IL-10 -1082G>A polymorphisms with atopic diseases with adjustment for confounding lifestyle and environmental factors. Methods: This study was performed on 356 Croatian students. The diagnosis of atopic asthma, atopic rhinitis and atopic dermatitis was based on symptoms reported by the modified International Study of Asthma and Allergies in Childhood questionnaire and a positive skin prick test (SPT) to at least one common inhalatory allergen. Genetic polymorphisms were genotyped using the polymerase chain reaction-based technique. The influence of personal (gender, body mass index, parental history of atopic disease), lifestyle (cigarette smoking, pet ownership) and environmental (urb...

za medicinska istraživanja i medicinu rada nastavak je izvješća objavljenih u prethodnim brojevim... more za medicinska istraživanja i medicinu rada nastavak je izvješća objavljenih u prethodnim brojevima ovog časopisa (1-5). U izvješću koje slijedi navode se osnovni statistički podaci o broju poziva primljenih u Centru za kontrolu otrovanja tijekom dvanaestomjesečnog razdoblja od siječnja do prosinca 2008. godine, uključujući podatke o tražiteljima informacija, kao i podatke o bolesnicima i osobinama otrovanja. Tijekom navedenog razdoblja u Centru je zabilježeno 1410 poziva kojima su se tražile informacije o 1446 slučaja. Pet poziva odnosilo se na moguću izloženost otrovnoj tvari u životinja. Tražene su informacije u vezi s izloženosti pesticidima u jednog psa, jedne koze i dvije ovce te otrovnom bilju u jednog psa.

Paediatria Croatica, 2014

Arhiv za Higijenu Rada i Toksikologiju, 2010

ABSTRACT Sputavanje djeteta tijekom medicinskih postupaka primjenjuje se iznimno, samo onda kada ... more ABSTRACT Sputavanje djeteta tijekom medicinskih postupaka primjenjuje se iznimno, samo onda kada su neuspješne sve druge mogućnosti. za uspješnost pojedinih medicinskih postupaka, nužno je mirovanje djeteta u posebnom namještaju tijela. Djrca ne razumiju razloge njihova sputavanja, stoga trpe jak stres.

Pathogenesis of atopic disorders such as atopic rhinitis, atopic asthma and atopic demratitis lie... more Pathogenesis of atopic disorders such as atopic rhinitis, atopic asthma and atopic demratitis lies in gene-environment interactions. Hypothesis of this study is that in Croatian population filaggrin gene (FLG) polymorphisms (R501X, 2282del4, R2447X i S3247X) contribute to atopic diseases, and that this contribution is modulated by environmental factors (settlement type, heating type, animal contact, smoking, physical activity, BMI, nutrition). The study enrolled 439 freshmen students at the University of Zagreb which underwent allergy testing, spirometry with bronchodilatation testing, FLG genotyping and answering a questionnaire regarding health, lifestyle and food intake. Results indicate low frequency of investigated FLG null mutations (2, 6%), thus being unconfirmed as significant etiological factors for atopy and atopic diseases in this population. However, significant risk for atopy was associated with male gender, parental family history of hay-fever and rodent pet exposure i...

Arhiv za higijenu rada i toksikologiju, 2010

Health continues previous reports published in this journal (1-6). It brings basic annual statist... more Health continues previous reports published in this journal (1-6). It brings basic annual statistics that include the number of calls received by the Poison Control Centre from January to December 2009, as well as information on callers, patients, and poisoning profi les. In 2009, the Poison Control Centre received 1532 phone calls reporting 1567 cases. Two calls were related to exposure in animals, i.e. exposure to foreign body in a dog and to pesticide methiocarb in a bird. In most cases (90 % of the total number of calls), the callers/information users were healthcare professionals (medical doctors and nurses) and only 10 % were public offi cials in other capacities (e.g. police) and general public. The average age of patients was 15 years (median 4 years), ranging from newborns to 85-year-olds, and the most prevalent age groups were the adults (39 % of the total number of patients with known age) and infants and preschool children (newborns to 5-year-olds; 36 %). As in earlier r...

Proteklih nekoliko desetljeca uocen je porast broja oboljelih od alergijskih bolesti u svijetu, a... more Proteklih nekoliko desetljeca uocen je porast broja oboljelih od alergijskih bolesti u svijetu, a osobito u razvijenim zemljama. Uzroci tog porasta nisu u potpunosti razjasnjeni, a neki od cinioca koji se povezuju s ucestalom pojavom alergijskih bolesti su oneciscenja okolisa te promjena u stilu života i prehrambenim navikama ljudi. Brojna istraživanja bave se mogucom ulogom proupalnih odnosno protuupalnih svojstava prehrambenih namirnica u patofiziologiji alergijske upalne reakcije. Esencijalne nezasicene masne kiseline kao sto su  -6 i  -3 masne kiseline u upalnom su procesu podvrgnute enzimskoj pretvorbi u upalne medijatore. Linolna kiselina( -6 ), redovito zastupljena u prehrani kroz margarin, ulja i sl., enzimatski se pretvara u arahidonsku kiselinu (AK), prekursora jakih upalnih medijatora leukotriena 4 i prostaglandina 2. Linolenska kiselina ( -3), zastupljena npr. u plavoj ribi, postiže protuupalno djelovanje enzimatskom pretvorbom u eikosapentaenoicnu kiselinu iz koje s...

International Journal of Food Sciences and Nutrition, 2013

The dietary intake of 12 nutrients with anti-oxidative properties (magnesium, zinc, copper, manga... more The dietary intake of 12 nutrients with anti-oxidative properties (magnesium, zinc, copper, manganese, selenium, vitamins A, C and E, ω-6 and ω-3 polyunsaturated fatty acids (PUFAs), flavonoids, and isoflavones), along with their major food sources, were analyzed by a food frequency questionnaire (FFQ) administered to 422 first-year students from the University of Zagreb, Croatia. Antioxidants intake in adolescents was generally sufficient, but intakes of magnesium and vitamin E were below 80% RDA in both sexes. Above-TUIL vitamin A intake was found in 13% of male and 3% of female adolescents with intakes up to 1400% RDA. Regional differences were significant, with higher intakes of magnesium, copper and selenium in females and lower ω-6/ω-3 PUFAs ratio in both genders in coastal compared to continental region. In conclusion, this study indicates the need for gender- and region-specific educational strategies for implementation of a healthier diet among Croatian adolescents.

Background: Null mutations in the gene encoding filaggrin (FLG), which result in the loss of fila... more Background: Null mutations in the gene encoding filaggrin (FLG), which result in the loss of filaggrin production and hence disrupt the epidermal barrier function, have been strongly associated with atopic dermatitis and present a predisposing factor in the development of the atopic march. The frequencies of the most common null mutations among the Caucasian population in Western Europe and North America were analyzed in young adult Croatian population, and their relation to skin and respiratory atopic diseases was assessed. Method: FLG null mutations R501X, 2282del4, R2447X and S3247X were genotyped in 423 students (305 females and 118 males, median age 19 years) with defined atopic phenotype (atopic dermatitis, rhinitis and asthma) by means of recorded atopic skin and respiratory symptoms with modified ISAAC questionnaire and positive skin prick testing (SPT) to one or more common inhalatory allergens. Result: We found 11 FLG null mutations carriers, 1/423 (0.2%) heterozygous for ...

Inflammatory cytokines are implicated in the pathophysiology of both stroke and depression, and t... more Inflammatory cytokines are implicated in the pathophysiology of both stroke and depression, and their production is influenced by the transcriptional activity of particular gene polymorphisms. We hypothesised that alleles related to higher pro-inflammatory and/or lower anti-inflammatory cytokine production would be associated with post-stroke depression (PSD). In 276 stroke cases, depression was diagnosed using DSM-IV, and classified into major PSD (N = 29), all (major plus minor) PSD (N = 77), and control (N = 199) groups. Genotyping for six pro-inflammatory polymorphisms (TNF-α -850C/T and -308G/A, IL-1β -511C/T and + 3953C/T, IL-6 -174G/C, and IL-8 -251T/A) and two anti-inflammatory polymorphisms (IL-4 + 33T/C and IL-10 -1082G/A) was conducted. Individual associations with PSD were estimated using logistic regression models. Total numbers of potential risk alleles were calculated for pro-inflammatory and anti-inflammatory cytokine genes and analysed against depression using χ(2)-tests. The IL-4 + 33C/C genotype was associated with major PSD, and the IL-10 -1082A/A genotype was associated with all PSD. Increasing numbers of risk alleles for these two anti-inflammatory cytokine genotypes were significantly associated with both PSD categories. No significant associations were found with any pro-inflammatory cytokine allele. Alleles associated with reduced anti-inflammatory cytokine function were associated with PSD, supporting the cytokine hypothesis in its etiology.

Institut za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska Dosadašnja istraživanja pok... more Institut za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska Dosadašnja istraživanja pokazuju da su null-mutacije gena, odnosno gubitak funkcije gena koji kodira protein fi lagrin (FLG), čija je funkcija održavanje strukture i hidracije epidermisa, povezane s nastankom atopijskih poremećaja, ponajprije sa sindromom atopijskog ekcema/dermatitisa (AEDS). Sveukupno je dosad izolirano 40-tak različitih null-mutacija FLG čiji udio varira među svjetskim populacijama. U zapadnoj Europi i Sjevernoj Americi null-mutacije FLG su prisutne u 10% populacije bijele rase od čega su najčešće mutacije R501X i 2282del4. Rezultati objavljenih europskih studija ukazuju da rasprostranjenost mutacija R501X i 2282del4 FLG ovisi o geografskoj širini, tj. da postoji gradijent učestalosti navedenih mutacija od sjevera prema jugu. Koža nosioca null-mutacije FLG podložnija je utjecaju različitih štetnosti te poremećena kožna barijera uzrokovana mutacijama FLG može biti dostatna za razvoj nespecifi čnih kožnih simptoma povezanih s atopijskim i neatopijskim kožnim poremećajima. Epidemiološke studije ukazuju na povezanost null-mutacija FLG s AEDS, dok rezultati o povezanosti null-mutacija FLG s razvojem senzibilizacije na uobičajene inhalacijske alergene, razvojem rinitisa i astme neovisno o prisutnosti AEDS nisu jednoznačni. U hrvatskoj populaciji utvrđena je mala učestalost nullmutacija FLG (2,6 %), te one nisu potvrđene kao značajni etiološki čimbenici u pojavi atopije i atopijskih bolesti u ispitivanoj populaciji.