Yuriy Ivanov - Academia.edu (original) (raw)

Papers by Yuriy Ivanov

Research paper thumbnail of Martian dust storm impact on atmospheric H2O and D/H observed by ExoMars Trace Gas Orbiter

Nature, 2019

Global dust storms develop at Mars at irregular intervals of several years 1,2. They have major e... more Global dust storms develop at Mars at irregular intervals of several years 1,2. They have major effects, causing an inflation of the atmosphere and changes in the dynamical behaviour, primarily due to solar heating of the dust. Recently published observations of Mars' atmospheric water abundance during dust storm conditions revealed a high-altitude increase, more pronounced at high northern latitudes 3,4 , and a decrease in the water column at low latitudes 5,6. These results, however, lacked concurrent measurements of atmospheric dust loading 3 , had a poor vertical resolution 3 , or were indirect 4. The start of science operations with the ESA/ROSCOSMOS ExoMars Trace Gas Orbiter spacecraft coincided with the onset of a global dust storm on Mars. We provide new evidence of the

Research paper thumbnail of The power hyperspectral all-sky camera

Research paper thumbnail of The Venus infrared atmospheric gases linker instrument concept for solar occultation studies of Venus atmosphere composition and structure onboard the Venus Orbiter Mission of the Indian Space Research Organization

Optics, Photonics and Digital Technologies for Imaging Applications VII, Mar 30, 2022

Research paper thumbnail of Infrared Spectrometer for ExoMars: A Mast-Mounted Instrument for the Rover

Astrobiology

ISEM (Infrared Spectrometer for ExoMars) is a pencil-beam infrared spectrometer that will measure... more ISEM (Infrared Spectrometer for ExoMars) is a pencil-beam infrared spectrometer that will measure reflected solar radiation in the near infrared range for context assessment of the surface mineralogy in the vicinity of the ExoMars rover. The instrument will be accommodated on the mast of the rover and will be operated together with the panoramic camera (PanCam), high-resolution camera (HRC). ISEM will study the mineralogical and petrographic composition of the martian surface in the vicinity of the rover, and in combination with the other remote sensing instruments, it will aid in the selection of potential targets for close-up investigations and drilling sites. Of particular scientific interest are water-bearing minerals, such as phyllosilicates, sulfates, carbonates, and minerals indicative of astrobiological potential, such as borates, nitrates, and ammonium-bearing minerals. The instrument has an ∼1° field of view and covers the spectral range between 1.15 and 3.30 μm with a spe...

Research paper thumbnail of Infrared Spectrometer for ExoMars: A Mast-Mounted Instrument for the Rover

Astrobiology

ISEM (Infrared Spectrometer for ExoMars) is a pencil-beam infrared spectrometer that will measure... more ISEM (Infrared Spectrometer for ExoMars) is a pencil-beam infrared spectrometer that will measure reflected solar radiation in the near infrared range for context assessment of the surface mineralogy in the vicinity of the ExoMars rover. The instrument will be accommodated on the mast of the rover and will be operated together with the panoramic camera (PanCam), high-resolution camera (HRC). ISEM will study the mineralogical and petrographic composition of the martian surface in the vicinity of the rover, and in combination with the other remote sensing instruments, it will aid in the selection of potential targets for close-up investigations and drilling sites. Of particular scientific interest are water-bearing minerals, such as phyllosilicates, sulfates, carbonates, and minerals indicative of astrobiological potential, such as borates, nitrates, and ammonium-bearing minerals. The instrument has an ∼1° field of view and covers the spectral range between 1.15 and 3.30 μm with a spe...

Research paper thumbnail of Russian contribution to ExoMars Trace Gas Orbiter: Atmospheric Chemistry Suite (ACS)

EGU General Assembly Conference Abstracts, Apr 1, 2016

Research paper thumbnail of Optical design of imaging spectrometer for atmosphere monitoring from near-Earth orbit

Optical Design and Engineering VII, 2018

Research paper thumbnail of Optical design and modeling of satellite imaging spectrometer for atmosphere monitoring

International Conference on Space Optics — ICSO 2018, 2019

Research paper thumbnail of Acousto-optic spectrometer ISEM for ExoMars-2020 space mission: ground measurements and calibrations

We describe near-infrared acousto-optic (AO) spectrometer ISEM (Infrared Spectrometer for ExoMars... more We describe near-infrared acousto-optic (AO) spectrometer ISEM (Infrared Spectrometer for ExoMars) developed for ExoMars 2020 space mission. The instrument goal is to investigate Martian surface and in particular to study mineralogical and, probably, petrographic composition of the uppermost regolith layer of the regolith by measuring reflected solar radiation in the near infrared spectral range. The instrument covers the wavelength range from 1.15 to 3.3 μm with the spectral resolution of ~25 cm-1 and has a circular field of view (FOW) of about 1°. The spectrometer consists of two parts: optical box and electronic box. The optical box is to be mounted on the top of the Martian rover’s mast providing better field of vision. Here we present the instrument description and optical design as well as the first results of laboratory calibrations and ground-based measurements of the Martian analogue samples.

Research paper thumbnail of Publisher Correction: Martian dust storm impact on atmospheric H2O and D/H observed by ExoMars Trace Gas Orbiter

Research paper thumbnail of <title>Device and program complex for the remote spectrophotometric monitoring of the gas composition of Earth's atmosphere</title>

23rd European Meeting on Atmospheric Studies by Optical Methods, 1997

ABSTRACT We have shown that even now it is possible only by the efforts of Ukrainian enterprises ... more ABSTRACT We have shown that even now it is possible only by the efforts of Ukrainian enterprises to make compact and vibrostable Fourier spectrometers which can be placed not only in laboratories (stationary and movable), but on the board of artificial satellite of the Earth. The spectrophotometry in thermal region of the spectrum is perspective remote method both for monitoring the dynamics of already known gaseous component and discovery of new ones. Within the framework of National program &#39;The system of ecological monitoring &#39;Ukraine&#39;&#39; it is reasonable to create the system of the same type movable laboratories for monitoring already ecologically contaminated regions as well as yet relatively undangerous ones.

Research paper thumbnail of Hyperspectral all-sky imaging of auroras

Optics Express, 2012

A prototype auroral hyperspectral all-sky camera has been constructed and tested. It uses electro... more A prototype auroral hyperspectral all-sky camera has been constructed and tested. It uses electro-optical tunable filters to image the night sky as a function of wavelength throughout the visible spectrum with no moving mechanical parts. The core optical system includes a new high power all-sky lens with F-number equal to f/1.1. The camera has been tested at the Kjell Henriksen Observatory (KHO) during the auroral season of 2011/2012. It detects all sub classes of aurora above ~½ of the sub visual 1kR green intensity threshold at an exposure time of only one second. Supervised classification of the hyperspectral data shows promise as a new method to process and identify auroral forms.

Research paper thumbnail of Scan Mirror Assembly for the Multispectral Scanning Polarimeter of Aerosol-UA Space Mission

International Journal of Optics

In this paper, the scan mirror assembly for the space experiment Aerosol-UA scanning polarimeter ... more In this paper, the scan mirror assembly for the space experiment Aerosol-UA scanning polarimeter (ScanPol) is described. The aim of the Ukrainian space mission Aerosol-UA is to create a database of the optical characteristics of aerosol and cloud particles in the Earth’s atmosphere over a long period of time. The optical characteristics of aerosol and cloud particles are derived from multiangular measurements. Multiangular scanning in ScanPol is provided by scan mirror assembly, which contains a reactive torque compensator electric motor and two scan mirrors, mounted on the shaft of the motor. The control system of the scan mirror assembly enables continuous scanning with a constant speed of the space under investigation. This control system tolerates movements of the orbiting satellite and preserves invariability of its spatial position. The polarimeter ScanPol is designed to acquire spatial, temporal, and spectral-polarimetric measurements simultaneously to minimize instrumental e...

Research paper thumbnail of Scan Mirror Assembly for the Multispectral Scanning Polarimeter of Aerosol-UA Space Mission

International Journal of Optics

In this paper, the scan mirror assembly for the space experiment Aerosol-UA scanning polarimeter ... more In this paper, the scan mirror assembly for the space experiment Aerosol-UA scanning polarimeter (ScanPol) is described. The aim of the Ukrainian space mission Aerosol-UA is to create a database of the optical characteristics of aerosol and cloud particles in the Earth’s atmosphere over a long period of time. The optical characteristics of aerosol and cloud particles are derived from multiangular measurements. Multiangular scanning in ScanPol is provided by scan mirror assembly, which contains a reactive torque compensator electric motor and two scan mirrors, mounted on the shaft of the motor. The control system of the scan mirror assembly enables continuous scanning with a constant speed of the space under investigation. This control system tolerates movements of the orbiting satellite and preserves invariability of its spatial position. The polarimeter ScanPol is designed to acquire spatial, temporal, and spectral-polarimetric measurements simultaneously to minimize instrumental e...

Research paper thumbnail of Multispectral Polarization State Analyzer of Scanning Polarimeter ScanPol

International Journal of Optics

We present the results of the development of a representational model of the multispectral polari... more We present the results of the development of a representational model of the multispectral polarization state analyzer of the ScanPol scanning polarimeter for the space experiment Aerosol-UA. The aim of the Ukrainian space mission Aerosol-UA is to create a database based on continuous satellite measurements of the optical characteristics of aerosol and cloud particles in the Earth’s atmosphere over a long period of time. The ScanPol polarimeter is designed to acquire spatial, temporal, and spectral-polarimetric measurements simultaneously to minimize instrumental “parasitic” effects and effects of “false” polarizations due to scene movement. Simultaneity is provided by separation of the initial spatial field by a pair of conjugated telescopes and a pair of Wollaston prisms. ScanPol provides to measure the first three Stokes parameters I, Q, and U of the radiation reflected by atmospheric aerosols and the Earth’s surface for a six solar reflectance spectral bands in the near ultravio...

Research paper thumbnail of Low-resolution spectro-polarimeter with broadband achromatic plate and special Wollaston prism

A new schema of an astronomical spectropolarimeter is suggested based on two original optical ele... more A new schema of an astronomical spectropolarimeter is suggested based on two original optical elements: 1) a superachromatic revolving retarder containing form 5 to 7 plastic plates to measure all four Stokes parameters in the range from 0.3 to 1.5 (mu) . 2) a complex Wollaston prism with decentered and deformed surfaces which allows to obtain the two spectra with

Research paper thumbnail of Design and field testing of the Fish-Eye lens for optical atmospheric observations

Proceedings of the International Astronomical Union, 2012

The Fish-Eye lens MAO-08 is intended for observations of weak extended objects (aurora, twilight ... more The Fish-Eye lens MAO-08 is intended for observations of weak extended objects (aurora, twilight and dawn phenomena, stratospheric clouds, etc.) in narrow spectral bands with variable passband filters VARISPEC and in white light. It is valuable for astronomical observation for the target – background problem when we need to estimate the spectral transparence of the atmosphere. Besides having high power this lens can be used for meteor observations. It has been tested during the winter field conditions.

Research paper thumbnail of Hyperspectral all-sky imaging of auroras

Optics Express, 2012

A prototype auroral hyperspectral all-sky camera has been constructed and tested. It uses electro... more A prototype auroral hyperspectral all-sky camera has been constructed and tested. It uses electro-optical tunable filters to image the night sky as a function of wavelength throughout the visible spectrum with no moving mechanical parts. The core optical system includes a new high power all-sky lens with F-number equal to f/1.1. The camera has been tested at the Kjell Henriksen Observatory (KHO) during the auroral season of 2011/2012. It detects all sub classes of aurora above ~½ of the sub visual 1kR green intensity threshold at an exposure time of only one second. Supervised classification of the hyperspectral data shows promise as a new method to process and identify auroral forms.

Research paper thumbnail of Analysis of the binding of bispecific monoclonal antibodies with immobilized antigens (human IgG and horseradish peroxidase) using a resonant mirror biosensor

Journal of Immunological Methods, 2002

The interaction between two monoclonal antibodies (mAbs) and their corresponding bispecific antib... more The interaction between two monoclonal antibodies (mAbs) and their corresponding bispecific antibody (bAb) with immobilized antigens has been examined using a resonant mirror biosensor (IAsys). BAbs were produced by cell fusion. The analysed panel of affinity-purified antibodies included two parental mAbs, one specific to human IgG (hIgG), and another specific to horseradish peroxidase (HRP), and a bAb derived thereof (anti-hIgG/HRP). The real-time analysis showed the drastic differences in the avidity of bivalent anti-HRP mAbs and anti-HRP shoulder of bAbs. Thus, the observed equilibrium association constant (K ass ) of anti-HRP mAbs was about 50 times higher that of anti-HRP shoulder of bAbs. The ratio of association rate constants (k ass ) of mAbs and bAbs was about two, due to the statistical factor of two binding sites per bivalent antibody molecule. However, the dissociation rate constant (k diss ) of anti-HRP shoulder of bAbs was 21 times higher k diss of anti-HRP mAbs. The comparison with the theoretical model shows that these observations are consistent only with a situation in which bivalent binding of mAbs with immobilized HRP predominates over monovalent binding. On the contrary, the second parental mAb (anti-hIgG) did not show the increase in avidity due to bivalent binding, compared to the anti-hIgG shoulder of bAbs, suggesting that this mAb was bound monovalently to immobilized hIgG. The K ass values determined by solid-phase radioimmunoassay (RIA) yielded figures almost overlapping with those obtained by IAsys. The results of the comparison of bAbs and mAbs are discussed from the viewpoint of the use of bAbs in heterogeneous systems. On the other hand, these data 0022-1759/02/$ -see front matter D 2002 Elsevier Science B.V. All rights reserved. PII: S 0 0 2 2 -1 7 5 9 ( 0 1 ) 0 0 5 5 8 -0 demonstrate that real-time analysis of antibody binding parameters in IAsys biosensor is valuable for the selection of mAbs and bAbs with desired features, for different fields of application. D

Research paper thumbnail of Martian dust storm impact on atmospheric H2O and D/H observed by ExoMars Trace Gas Orbiter

Nature, 2019

Global dust storms develop at Mars at irregular intervals of several years 1,2. They have major e... more Global dust storms develop at Mars at irregular intervals of several years 1,2. They have major effects, causing an inflation of the atmosphere and changes in the dynamical behaviour, primarily due to solar heating of the dust. Recently published observations of Mars' atmospheric water abundance during dust storm conditions revealed a high-altitude increase, more pronounced at high northern latitudes 3,4 , and a decrease in the water column at low latitudes 5,6. These results, however, lacked concurrent measurements of atmospheric dust loading 3 , had a poor vertical resolution 3 , or were indirect 4. The start of science operations with the ESA/ROSCOSMOS ExoMars Trace Gas Orbiter spacecraft coincided with the onset of a global dust storm on Mars. We provide new evidence of the

Research paper thumbnail of The power hyperspectral all-sky camera

Research paper thumbnail of The Venus infrared atmospheric gases linker instrument concept for solar occultation studies of Venus atmosphere composition and structure onboard the Venus Orbiter Mission of the Indian Space Research Organization

Optics, Photonics and Digital Technologies for Imaging Applications VII, Mar 30, 2022

Research paper thumbnail of Infrared Spectrometer for ExoMars: A Mast-Mounted Instrument for the Rover

Astrobiology

ISEM (Infrared Spectrometer for ExoMars) is a pencil-beam infrared spectrometer that will measure... more ISEM (Infrared Spectrometer for ExoMars) is a pencil-beam infrared spectrometer that will measure reflected solar radiation in the near infrared range for context assessment of the surface mineralogy in the vicinity of the ExoMars rover. The instrument will be accommodated on the mast of the rover and will be operated together with the panoramic camera (PanCam), high-resolution camera (HRC). ISEM will study the mineralogical and petrographic composition of the martian surface in the vicinity of the rover, and in combination with the other remote sensing instruments, it will aid in the selection of potential targets for close-up investigations and drilling sites. Of particular scientific interest are water-bearing minerals, such as phyllosilicates, sulfates, carbonates, and minerals indicative of astrobiological potential, such as borates, nitrates, and ammonium-bearing minerals. The instrument has an ∼1° field of view and covers the spectral range between 1.15 and 3.30 μm with a spe...

Research paper thumbnail of Infrared Spectrometer for ExoMars: A Mast-Mounted Instrument for the Rover

Astrobiology

ISEM (Infrared Spectrometer for ExoMars) is a pencil-beam infrared spectrometer that will measure... more ISEM (Infrared Spectrometer for ExoMars) is a pencil-beam infrared spectrometer that will measure reflected solar radiation in the near infrared range for context assessment of the surface mineralogy in the vicinity of the ExoMars rover. The instrument will be accommodated on the mast of the rover and will be operated together with the panoramic camera (PanCam), high-resolution camera (HRC). ISEM will study the mineralogical and petrographic composition of the martian surface in the vicinity of the rover, and in combination with the other remote sensing instruments, it will aid in the selection of potential targets for close-up investigations and drilling sites. Of particular scientific interest are water-bearing minerals, such as phyllosilicates, sulfates, carbonates, and minerals indicative of astrobiological potential, such as borates, nitrates, and ammonium-bearing minerals. The instrument has an ∼1° field of view and covers the spectral range between 1.15 and 3.30 μm with a spe...

Research paper thumbnail of Russian contribution to ExoMars Trace Gas Orbiter: Atmospheric Chemistry Suite (ACS)

EGU General Assembly Conference Abstracts, Apr 1, 2016

Research paper thumbnail of Optical design of imaging spectrometer for atmosphere monitoring from near-Earth orbit

Optical Design and Engineering VII, 2018

Research paper thumbnail of Optical design and modeling of satellite imaging spectrometer for atmosphere monitoring

International Conference on Space Optics — ICSO 2018, 2019

Research paper thumbnail of Acousto-optic spectrometer ISEM for ExoMars-2020 space mission: ground measurements and calibrations

We describe near-infrared acousto-optic (AO) spectrometer ISEM (Infrared Spectrometer for ExoMars... more We describe near-infrared acousto-optic (AO) spectrometer ISEM (Infrared Spectrometer for ExoMars) developed for ExoMars 2020 space mission. The instrument goal is to investigate Martian surface and in particular to study mineralogical and, probably, petrographic composition of the uppermost regolith layer of the regolith by measuring reflected solar radiation in the near infrared spectral range. The instrument covers the wavelength range from 1.15 to 3.3 μm with the spectral resolution of ~25 cm-1 and has a circular field of view (FOW) of about 1°. The spectrometer consists of two parts: optical box and electronic box. The optical box is to be mounted on the top of the Martian rover’s mast providing better field of vision. Here we present the instrument description and optical design as well as the first results of laboratory calibrations and ground-based measurements of the Martian analogue samples.

Research paper thumbnail of Publisher Correction: Martian dust storm impact on atmospheric H2O and D/H observed by ExoMars Trace Gas Orbiter

Research paper thumbnail of <title>Device and program complex for the remote spectrophotometric monitoring of the gas composition of Earth's atmosphere</title>

23rd European Meeting on Atmospheric Studies by Optical Methods, 1997

ABSTRACT We have shown that even now it is possible only by the efforts of Ukrainian enterprises ... more ABSTRACT We have shown that even now it is possible only by the efforts of Ukrainian enterprises to make compact and vibrostable Fourier spectrometers which can be placed not only in laboratories (stationary and movable), but on the board of artificial satellite of the Earth. The spectrophotometry in thermal region of the spectrum is perspective remote method both for monitoring the dynamics of already known gaseous component and discovery of new ones. Within the framework of National program &#39;The system of ecological monitoring &#39;Ukraine&#39;&#39; it is reasonable to create the system of the same type movable laboratories for monitoring already ecologically contaminated regions as well as yet relatively undangerous ones.

Research paper thumbnail of Hyperspectral all-sky imaging of auroras

Optics Express, 2012

A prototype auroral hyperspectral all-sky camera has been constructed and tested. It uses electro... more A prototype auroral hyperspectral all-sky camera has been constructed and tested. It uses electro-optical tunable filters to image the night sky as a function of wavelength throughout the visible spectrum with no moving mechanical parts. The core optical system includes a new high power all-sky lens with F-number equal to f/1.1. The camera has been tested at the Kjell Henriksen Observatory (KHO) during the auroral season of 2011/2012. It detects all sub classes of aurora above ~½ of the sub visual 1kR green intensity threshold at an exposure time of only one second. Supervised classification of the hyperspectral data shows promise as a new method to process and identify auroral forms.

Research paper thumbnail of Scan Mirror Assembly for the Multispectral Scanning Polarimeter of Aerosol-UA Space Mission

International Journal of Optics

In this paper, the scan mirror assembly for the space experiment Aerosol-UA scanning polarimeter ... more In this paper, the scan mirror assembly for the space experiment Aerosol-UA scanning polarimeter (ScanPol) is described. The aim of the Ukrainian space mission Aerosol-UA is to create a database of the optical characteristics of aerosol and cloud particles in the Earth’s atmosphere over a long period of time. The optical characteristics of aerosol and cloud particles are derived from multiangular measurements. Multiangular scanning in ScanPol is provided by scan mirror assembly, which contains a reactive torque compensator electric motor and two scan mirrors, mounted on the shaft of the motor. The control system of the scan mirror assembly enables continuous scanning with a constant speed of the space under investigation. This control system tolerates movements of the orbiting satellite and preserves invariability of its spatial position. The polarimeter ScanPol is designed to acquire spatial, temporal, and spectral-polarimetric measurements simultaneously to minimize instrumental e...

Research paper thumbnail of Scan Mirror Assembly for the Multispectral Scanning Polarimeter of Aerosol-UA Space Mission

International Journal of Optics

In this paper, the scan mirror assembly for the space experiment Aerosol-UA scanning polarimeter ... more In this paper, the scan mirror assembly for the space experiment Aerosol-UA scanning polarimeter (ScanPol) is described. The aim of the Ukrainian space mission Aerosol-UA is to create a database of the optical characteristics of aerosol and cloud particles in the Earth’s atmosphere over a long period of time. The optical characteristics of aerosol and cloud particles are derived from multiangular measurements. Multiangular scanning in ScanPol is provided by scan mirror assembly, which contains a reactive torque compensator electric motor and two scan mirrors, mounted on the shaft of the motor. The control system of the scan mirror assembly enables continuous scanning with a constant speed of the space under investigation. This control system tolerates movements of the orbiting satellite and preserves invariability of its spatial position. The polarimeter ScanPol is designed to acquire spatial, temporal, and spectral-polarimetric measurements simultaneously to minimize instrumental e...

Research paper thumbnail of Multispectral Polarization State Analyzer of Scanning Polarimeter ScanPol

International Journal of Optics

We present the results of the development of a representational model of the multispectral polari... more We present the results of the development of a representational model of the multispectral polarization state analyzer of the ScanPol scanning polarimeter for the space experiment Aerosol-UA. The aim of the Ukrainian space mission Aerosol-UA is to create a database based on continuous satellite measurements of the optical characteristics of aerosol and cloud particles in the Earth’s atmosphere over a long period of time. The ScanPol polarimeter is designed to acquire spatial, temporal, and spectral-polarimetric measurements simultaneously to minimize instrumental “parasitic” effects and effects of “false” polarizations due to scene movement. Simultaneity is provided by separation of the initial spatial field by a pair of conjugated telescopes and a pair of Wollaston prisms. ScanPol provides to measure the first three Stokes parameters I, Q, and U of the radiation reflected by atmospheric aerosols and the Earth’s surface for a six solar reflectance spectral bands in the near ultravio...

Research paper thumbnail of Low-resolution spectro-polarimeter with broadband achromatic plate and special Wollaston prism

A new schema of an astronomical spectropolarimeter is suggested based on two original optical ele... more A new schema of an astronomical spectropolarimeter is suggested based on two original optical elements: 1) a superachromatic revolving retarder containing form 5 to 7 plastic plates to measure all four Stokes parameters in the range from 0.3 to 1.5 (mu) . 2) a complex Wollaston prism with decentered and deformed surfaces which allows to obtain the two spectra with

Research paper thumbnail of Design and field testing of the Fish-Eye lens for optical atmospheric observations

Proceedings of the International Astronomical Union, 2012

The Fish-Eye lens MAO-08 is intended for observations of weak extended objects (aurora, twilight ... more The Fish-Eye lens MAO-08 is intended for observations of weak extended objects (aurora, twilight and dawn phenomena, stratospheric clouds, etc.) in narrow spectral bands with variable passband filters VARISPEC and in white light. It is valuable for astronomical observation for the target – background problem when we need to estimate the spectral transparence of the atmosphere. Besides having high power this lens can be used for meteor observations. It has been tested during the winter field conditions.

Research paper thumbnail of Hyperspectral all-sky imaging of auroras

Optics Express, 2012

A prototype auroral hyperspectral all-sky camera has been constructed and tested. It uses electro... more A prototype auroral hyperspectral all-sky camera has been constructed and tested. It uses electro-optical tunable filters to image the night sky as a function of wavelength throughout the visible spectrum with no moving mechanical parts. The core optical system includes a new high power all-sky lens with F-number equal to f/1.1. The camera has been tested at the Kjell Henriksen Observatory (KHO) during the auroral season of 2011/2012. It detects all sub classes of aurora above ~½ of the sub visual 1kR green intensity threshold at an exposure time of only one second. Supervised classification of the hyperspectral data shows promise as a new method to process and identify auroral forms.

Research paper thumbnail of Analysis of the binding of bispecific monoclonal antibodies with immobilized antigens (human IgG and horseradish peroxidase) using a resonant mirror biosensor

Journal of Immunological Methods, 2002

The interaction between two monoclonal antibodies (mAbs) and their corresponding bispecific antib... more The interaction between two monoclonal antibodies (mAbs) and their corresponding bispecific antibody (bAb) with immobilized antigens has been examined using a resonant mirror biosensor (IAsys). BAbs were produced by cell fusion. The analysed panel of affinity-purified antibodies included two parental mAbs, one specific to human IgG (hIgG), and another specific to horseradish peroxidase (HRP), and a bAb derived thereof (anti-hIgG/HRP). The real-time analysis showed the drastic differences in the avidity of bivalent anti-HRP mAbs and anti-HRP shoulder of bAbs. Thus, the observed equilibrium association constant (K ass ) of anti-HRP mAbs was about 50 times higher that of anti-HRP shoulder of bAbs. The ratio of association rate constants (k ass ) of mAbs and bAbs was about two, due to the statistical factor of two binding sites per bivalent antibody molecule. However, the dissociation rate constant (k diss ) of anti-HRP shoulder of bAbs was 21 times higher k diss of anti-HRP mAbs. The comparison with the theoretical model shows that these observations are consistent only with a situation in which bivalent binding of mAbs with immobilized HRP predominates over monovalent binding. On the contrary, the second parental mAb (anti-hIgG) did not show the increase in avidity due to bivalent binding, compared to the anti-hIgG shoulder of bAbs, suggesting that this mAb was bound monovalently to immobilized hIgG. The K ass values determined by solid-phase radioimmunoassay (RIA) yielded figures almost overlapping with those obtained by IAsys. The results of the comparison of bAbs and mAbs are discussed from the viewpoint of the use of bAbs in heterogeneous systems. On the other hand, these data 0022-1759/02/$ -see front matter D 2002 Elsevier Science B.V. All rights reserved. PII: S 0 0 2 2 -1 7 5 9 ( 0 1 ) 0 0 5 5 8 -0 demonstrate that real-time analysis of antibody binding parameters in IAsys biosensor is valuable for the selection of mAbs and bAbs with desired features, for different fields of application. D