Jérôme Cayla - Academia.edu (original) (raw)
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![Research paper thumbnail of Differential influence of propofol and isoflurane anesthesia in non-human primate on the brain kinetics and binding of [ 18 F]DPA-714, a PET imaging marker of glial activation](https://a.academia-assets.com/images/blank-paper.jpg)
](European Journal of Neuroscience, 2015
Translocator protein 18kDa (TSPO) expression at the mitochondrial membrane of glial cells is rela... more Translocator protein 18kDa (TSPO) expression at the mitochondrial membrane of glial cells is related to glial activation. TSPO radioligands such as [(18) F]DPA-714 are useful for the non-invasive study of neuroimmune processes using positron emission tomography (PET). Anesthetic agents were shown to impact mitochondrial function and may influence [(18) F]DPA-714 binding parameters and PET kinetics. [(18) F]DPA-714 PET imaging was performed in Papio Anubis baboons anesthetized using either intravenous propofol (n=3) or inhaled isoflurane (n=3). Brain kinetics and metabolite-corrected input function were measured to estimate [(18) F]DPA-714 brain distribution (VT ). Displacement experiments were performed using PK11195 (1.5 mg/kg). In vitro [(18) F]DPA-714 binding experiments were performed using baboon brain tissue in the absence and the presence of tested anesthetics. Brain radioactivity peaked higher in isoflurane anesthetized animals compared to propofol (SUVmax =2.7±0.5 versus 1.3±0.2, respectively) but was not different after 30 min. Brain VT was not different under propofol and isoflurane. Displacement resulted in a 35.8±8.4 % decrease of brain radioactivity under propofol but not under isoflurane (0.1±7.0 %). In vitro, the presence of propofol increased TSPO density and dramatically reduced its affinity for [(18) F]DPA-714 compared to control. This in vitro effect was not significant with isoflurane. Exposure to propofol and isoflurane differentially influences TSPO interaction with its specific radioligand [18F]DPA-714 with subsequent impact on its tissue kinetics and specific binding estimated in vivo using PET. Therefore, the choice of anesthetics and their potential influence on PET data should be considered for the design of imaging studies using TSPO radioligands, especially in a translational research context. This article is protected by copyright. All rights reserved.
![Research paper thumbnail of In vivo visualization of central muscarinic receptors using [11C]quinuclidinyl benzilate and positron emission tomography in baboons](https://attachments.academia-assets.com/40367348/thumbnails/1.jpg)
](European Journal of Pharmacology, 1992
The muscarinic antagonist, quinuclidinyl benzilate (QNB), labeled with carbon 11 was used as a ra... more The muscarinic antagonist, quinuclidinyl benzilate (QNB), labeled with carbon 11 was used as a radioligand to visualize in vivo by positron emission tomography (PET) the central muscarinic acetylcholine receptors (mAChR) in baboons (Papio papio).
European Journal of Neuroscience, 2015
Translocator protein 18kDa (TSPO) expression at the mitochondrial membrane of glial cells is rela... more Translocator protein 18kDa (TSPO) expression at the mitochondrial membrane of glial cells is related to glial activation. TSPO radioligands such as [(18) F]DPA-714 are useful for the non-invasive study of neuroimmune processes using positron emission tomography (PET). Anesthetic agents were shown to impact mitochondrial function and may influence [(18) F]DPA-714 binding parameters and PET kinetics. [(18) F]DPA-714 PET imaging was performed in Papio Anubis baboons anesthetized using either intravenous propofol (n=3) or inhaled isoflurane (n=3). Brain kinetics and metabolite-corrected input function were measured to estimate [(18) F]DPA-714 brain distribution (VT ). Displacement experiments were performed using PK11195 (1.5 mg/kg). In vitro [(18) F]DPA-714 binding experiments were performed using baboon brain tissue in the absence and the presence of tested anesthetics. Brain radioactivity peaked higher in isoflurane anesthetized animals compared to propofol (SUVmax =2.7±0.5 versus 1.3±0.2, respectively) but was not different after 30 min. Brain VT was not different under propofol and isoflurane. Displacement resulted in a 35.8±8.4 % decrease of brain radioactivity under propofol but not under isoflurane (0.1±7.0 %). In vitro, the presence of propofol increased TSPO density and dramatically reduced its affinity for [(18) F]DPA-714 compared to control. This in vitro effect was not significant with isoflurane. Exposure to propofol and isoflurane differentially influences TSPO interaction with its specific radioligand [18F]DPA-714 with subsequent impact on its tissue kinetics and specific binding estimated in vivo using PET. Therefore, the choice of anesthetics and their potential influence on PET data should be considered for the design of imaging studies using TSPO radioligands, especially in a translational research context. This article is protected by copyright. All rights reserved.
European Journal of Pharmacology, 1992
The muscarinic antagonist, quinuclidinyl benzilate (QNB), labeled with carbon 11 was used as a ra... more The muscarinic antagonist, quinuclidinyl benzilate (QNB), labeled with carbon 11 was used as a radioligand to visualize in vivo by positron emission tomography (PET) the central muscarinic acetylcholine receptors (mAChR) in baboons (Papio papio).