Júlia Lemos - Academia.edu (original) (raw)
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Papers by Júlia Lemos
Frontiers in Immunology, Apr 15, 2021
BMC Infectious Diseases, 2019
Background Zika virus (ZIKV) infection gained public health concern after the 2015 outbreak in Br... more Background Zika virus (ZIKV) infection gained public health concern after the 2015 outbreak in Brazil, when microcephaly rates increased in babies born from infected mothers. It was demonstrated that ZIKV causes a congenital Zika virus syndrome, including various alterations in the development of the central nervous system. Although the infection of cells from the nervous system has been well documented, less is known in respect of ZIKV ability to infect immune cells. Herein, we investigated if peripheral blood mononuclear cells (PBMCs), freshly-isolated from healthy donors, could be infected by ZIKV. Methods PBMCs from healthy donors were isolated and cultured in medium with ZIKV strain Rio-U1 (MOI = 0.1). Infection was analyzed by RT-qPCR and flow cytometry. Results We detected the ZIKV RNA in PBMCs from all donors by RT-qPCR analysis. The detection of viral antigens by flow cytometry revealed that PBMC from more than 50% the donors were infected by ZIKV, with CD3+CD4+ T cells, CD...
Neuron, Jun 1, 2016
Striatal medium spiny neurons (MSNs) form inhibitory synapses on neighboring striatal neurons thr... more Striatal medium spiny neurons (MSNs) form inhibitory synapses on neighboring striatal neurons through axon collaterals. The functional relevance of this lateral inhibition and its regulation by dopamine remains elusive. We show that synchronized stimulation of collateral transmission from multiple indirect-pathway MSNs (iMSNs) potently inhibits action potentials in direct-pathway MSNs (dMSNs) in the nucleus accumbens. Dopamine D2 receptors (D2Rs) suppress lateral inhibition from iMSNs to disinhibit dMSNs, which are known to facilitate locomotion. Surprisingly, D2R inhibition of synaptic transmission was larger at axon collaterals from iMSNs than their projections to the ventral pallidum. Targeted deletion of D2Rs from iMSNs impaired cocaine's ability to suppress lateral inhibition and increase locomotion. These impairments were rescued by chemogenetic activation of Gi-signaling in iMSNs. These findings shed light on the functional significance of lateral inhibition between MSNs ...
Neuropsychopharmacology, 2010
The Journal of Neuroscience, 2020
Acta Scientiarum. Health Sciences, 2017
The objective of this study was to evaluate the knowledge of pediatricians on the integrality of ... more The objective of this study was to evaluate the knowledge of pediatricians on the integrality of attention to oral health in early childhood. A cross-sectional study was conducted with a sample consisting of 70 doctors, working in public and private services, in the city of Belém, state of Pará, Brazil. Data were collected using a questionnaire with approaches on: the professional profile; the knowledge of oral health of children; the use of fluoride; and self-perception on knowledge of oral health. A descriptive analysis of the data was performed, and the association between variables was verified with the Chi-square test, with p = 0.05. The average age of respondents was 41 years. Most professionals were females (88.57%) and featured more than 20 years of professional experience (38.57%). In general, most pediatricians answered satisfactorily the questions related to knowledge of children's oral health. However, when questioned about the knowledge of the use of fluoride, most ...
Frontiers in Immunology, Apr 15, 2021
BMC Infectious Diseases, 2019
Background Zika virus (ZIKV) infection gained public health concern after the 2015 outbreak in Br... more Background Zika virus (ZIKV) infection gained public health concern after the 2015 outbreak in Brazil, when microcephaly rates increased in babies born from infected mothers. It was demonstrated that ZIKV causes a congenital Zika virus syndrome, including various alterations in the development of the central nervous system. Although the infection of cells from the nervous system has been well documented, less is known in respect of ZIKV ability to infect immune cells. Herein, we investigated if peripheral blood mononuclear cells (PBMCs), freshly-isolated from healthy donors, could be infected by ZIKV. Methods PBMCs from healthy donors were isolated and cultured in medium with ZIKV strain Rio-U1 (MOI = 0.1). Infection was analyzed by RT-qPCR and flow cytometry. Results We detected the ZIKV RNA in PBMCs from all donors by RT-qPCR analysis. The detection of viral antigens by flow cytometry revealed that PBMC from more than 50% the donors were infected by ZIKV, with CD3+CD4+ T cells, CD...
Neuron, Jun 1, 2016
Striatal medium spiny neurons (MSNs) form inhibitory synapses on neighboring striatal neurons thr... more Striatal medium spiny neurons (MSNs) form inhibitory synapses on neighboring striatal neurons through axon collaterals. The functional relevance of this lateral inhibition and its regulation by dopamine remains elusive. We show that synchronized stimulation of collateral transmission from multiple indirect-pathway MSNs (iMSNs) potently inhibits action potentials in direct-pathway MSNs (dMSNs) in the nucleus accumbens. Dopamine D2 receptors (D2Rs) suppress lateral inhibition from iMSNs to disinhibit dMSNs, which are known to facilitate locomotion. Surprisingly, D2R inhibition of synaptic transmission was larger at axon collaterals from iMSNs than their projections to the ventral pallidum. Targeted deletion of D2Rs from iMSNs impaired cocaine's ability to suppress lateral inhibition and increase locomotion. These impairments were rescued by chemogenetic activation of Gi-signaling in iMSNs. These findings shed light on the functional significance of lateral inhibition between MSNs ...
Neuropsychopharmacology, 2010
The Journal of Neuroscience, 2020
Acta Scientiarum. Health Sciences, 2017
The objective of this study was to evaluate the knowledge of pediatricians on the integrality of ... more The objective of this study was to evaluate the knowledge of pediatricians on the integrality of attention to oral health in early childhood. A cross-sectional study was conducted with a sample consisting of 70 doctors, working in public and private services, in the city of Belém, state of Pará, Brazil. Data were collected using a questionnaire with approaches on: the professional profile; the knowledge of oral health of children; the use of fluoride; and self-perception on knowledge of oral health. A descriptive analysis of the data was performed, and the association between variables was verified with the Chi-square test, with p = 0.05. The average age of respondents was 41 years. Most professionals were females (88.57%) and featured more than 20 years of professional experience (38.57%). In general, most pediatricians answered satisfactorily the questions related to knowledge of children's oral health. However, when questioned about the knowledge of the use of fluoride, most ...