JABBAR Hamady - Academia.edu (original) (raw)
Papers by JABBAR Hamady
Wasit journal for pure sciences, Mar 30, 2024
Journal of Bioanalysis & Biomedicine, Jul 1, 2017
Liver is a metabolically active organ responsible for many vital life functions. It performs many... more Liver is a metabolically active organ responsible for many vital life functions. It performs many activities that are critical for survival. Due to its important activities, the liver is exposed to a number of insults and is one of the body's organs most subject to injury. In spite of tremendous advances in modern medicine, there are hardly any reliable drugs that protect the liver from damage and/or help in regeneration of hepatic cell. It is, therefore, necessary to search for effective and safe herbal drugs for the treatment of liver disease to replace currently used drugs of doubtful efficacy and safety. The aim of this study is to investigate hepatoprotective activity of aqueous extract of Nigella sativa seed in highly active antiretroviral therapy (Lamivudine, Zidovudine and Efavirenz) administered rats. Sixty rats weighed between 150-200g were randomly divided into six groups and each group comprised of ten rats. Rats in group I were administered with distilled water. Rats in group II were administered with highly active antiretroviral therapy only. Rats in groups III - VI were administered 100, 200, 400 and 800 mg/kg Nigella sativa plus highly active antiretroviral therapy respectively. The treatments were given orally for 28 consecutive days. On the 29th day, all rats were sacrificed under light diethyl ether anaesthesia; blood samples were collected for the assessment of biochemical parameters, while liver tissue was used for histopathological assessment. Serum levels of liver enzymes ALT, AST, ALP, and GGT were significantly (p<0.05) increased and albumin concentration was significantly decreased in animals treated with highly active antiretroviral therapy as compared to the normal control. Histopathological observations also revealed severe damage in the structure of liver tissue in animals administered with highly active antiretroviral therapy. Treatment of highly active antiretroviral therapy exposed animals with Nigella sativa showed marked improvement in both biochemical and histopathological findings. Rise in liver enzymes was almost restored to normal in animals treated with Nigella sativa. Nigella sativa through its antioxidant activity effectively protects highly active antiretroviral therapy induced liver toxicity.
Journal of Applied Pharmaceutical Science, 2016
To examine the effect of crude methanolic extract and phenolic compounds obtained from Nigella sa... more To examine the effect of crude methanolic extract and phenolic compounds obtained from Nigella sativa seeds on testicular expression levels of inhibin-α,-βA, and-βB subunits of intact mature male rats, thirty males were randomly assigned to three equal groups (C, M, and P). Males were orally administered with distal water, methanolic extract of N. sativa seed at the dose of 1.0 g/ kg b.w., and phenolic extract of N. sativa seed at the dose of 0.3 g/ kg b. w., daily for 18 days. At the end of treatment period, male rats were anesthetized by single injection of thiopental (100 mg/ kg, i.p.), sacrificed, and testes were removed. Samples from testicular tissues were quickly dipped in DEPC solution and frozen at-80 ºC until gene expression levels assessment of inhibin subunits by using semi-quantitative RT-PCR analyses. Male rats that have been treated with phenolic compounds (P group) registered marked increase of all inhibin subunits compared with those treated with methanolic extract, which they were also higher than that of control males. It can be concluded that phenolic compounds of N. sativa seeds have the more efficient role in reproductive functions than methanolic extract of N. sativa seed.
journal of applied pharmaceutical science, 2016
To examine the effect of crude methanolic extract and phenolic compounds obtained from Nigella sa... more To examine the effect of crude methanolic extract and phenolic compounds obtained from Nigella sativa seeds on testicular expression levels of inhibin-α,-βA, and-βB subunits of intact mature male rats, thirty males were randomly assigned to three equal groups (C, M, and P). Males were orally administered with distal water, methanolic extract of N. sativa seed at the dose of 1.0 g/ kg b.w., and phenolic extract of N. sativa seed at the dose of 0.3 g/ kg b. w., daily for 18 days. At the end of treatment period, male rats were anesthetized by single injection of thiopental (100 mg/ kg, i.p.), sacrificed, and testes were removed. Samples from testicular tissues were quickly dipped in DEPC solution and frozen at-80 ºC until gene expression levels assessment of inhibin subunits by using semi-quantitative RT-PCR analyses. Male rats that have been treated with phenolic compounds (P group) registered marked increase of all inhibin subunits compared with those treated with methanolic extract, which they were also higher than that of control males. It can be concluded that phenolic compounds of N. sativa seeds have the more efficient role in reproductive functions than methanolic extract of N. sativa seed.
International journal of health sciences
Current study aimed to investigation the protective effects of zinc and vitamin D in attenuating ... more Current study aimed to investigation the protective effects of zinc and vitamin D in attenuating alveolar response, as anti-inflammatory and antioxidant, in nicotine stressed male rats. Thirty adult males Wistar rats, as a model of mammals, randomly assigned to five equal groups and treatment as follows for 14 days: Control (C) drenched vehicle without treatment, G1 = injected nicotine 1.5 mg/ kg b.w. i/P, G2 administrated orally of zinc 60 mg/ kg b.w., G3 = administrated orally of vitamin D 250 µg/ kg b.w. and G4 administrated orally both of zinc and vitamin D with same doses and stressed by nicotine 1.5 mg/ kg b.w. i/P. At the end of the experiment, rats were anesthetized blood samples have been collected, and then iron, cytokines (IL-6) and malondialdehyde (MDA) and glutathione peroxidase (GPX) were estimation in in serum and lung tissues. The result showed significant (p ≤ 0.5) increase in serum MDA in group G1 when compared with other groups. While significant (p ≤ 0.5) increas...
International journal of health sciences
The current study aimed to investigation the role of zinc and vitamin D in modulating alveolar re... more The current study aimed to investigation the role of zinc and vitamin D in modulating alveolar response of nicotine stress as a model of mammals male Wistar rats. Thirty mature males werekept at 23 ± 2 C°, randomly assigned to five equal groups and treatment for 14 days, C = Control drenched vehicle without treatment, G1 = injected with i/p nicotine 1.5 mg/ kg b.w., G2 = administrated orally of zinc 60 mg/ kgb.w., G3 = administrated orally of vitamin D 250 µg/ kg b.w., G4 = administrated orally both of zinc and vitamin D with same doses stressed and nicotine 1.5 mg/ kgb.w.i/p. Rats were anesthetized with ketamine at the end of the treatment period with xylazine and blood samples have been collected from the optical vein for estimation of serum ferritin and transferrin, then animals were sacrificed and lung was removed and weighted.All groups of rats had lung samples extracted rapidly, dipped in DEPC solution, and frozen under liquid nitrogen for determination of tight junction prote...
Wasit journal for pure sciences, Mar 30, 2024
Journal of Bioanalysis & Biomedicine, Jul 1, 2017
Liver is a metabolically active organ responsible for many vital life functions. It performs many... more Liver is a metabolically active organ responsible for many vital life functions. It performs many activities that are critical for survival. Due to its important activities, the liver is exposed to a number of insults and is one of the body's organs most subject to injury. In spite of tremendous advances in modern medicine, there are hardly any reliable drugs that protect the liver from damage and/or help in regeneration of hepatic cell. It is, therefore, necessary to search for effective and safe herbal drugs for the treatment of liver disease to replace currently used drugs of doubtful efficacy and safety. The aim of this study is to investigate hepatoprotective activity of aqueous extract of Nigella sativa seed in highly active antiretroviral therapy (Lamivudine, Zidovudine and Efavirenz) administered rats. Sixty rats weighed between 150-200g were randomly divided into six groups and each group comprised of ten rats. Rats in group I were administered with distilled water. Rats in group II were administered with highly active antiretroviral therapy only. Rats in groups III - VI were administered 100, 200, 400 and 800 mg/kg Nigella sativa plus highly active antiretroviral therapy respectively. The treatments were given orally for 28 consecutive days. On the 29th day, all rats were sacrificed under light diethyl ether anaesthesia; blood samples were collected for the assessment of biochemical parameters, while liver tissue was used for histopathological assessment. Serum levels of liver enzymes ALT, AST, ALP, and GGT were significantly (p<0.05) increased and albumin concentration was significantly decreased in animals treated with highly active antiretroviral therapy as compared to the normal control. Histopathological observations also revealed severe damage in the structure of liver tissue in animals administered with highly active antiretroviral therapy. Treatment of highly active antiretroviral therapy exposed animals with Nigella sativa showed marked improvement in both biochemical and histopathological findings. Rise in liver enzymes was almost restored to normal in animals treated with Nigella sativa. Nigella sativa through its antioxidant activity effectively protects highly active antiretroviral therapy induced liver toxicity.
Journal of Applied Pharmaceutical Science, 2016
To examine the effect of crude methanolic extract and phenolic compounds obtained from Nigella sa... more To examine the effect of crude methanolic extract and phenolic compounds obtained from Nigella sativa seeds on testicular expression levels of inhibin-α,-βA, and-βB subunits of intact mature male rats, thirty males were randomly assigned to three equal groups (C, M, and P). Males were orally administered with distal water, methanolic extract of N. sativa seed at the dose of 1.0 g/ kg b.w., and phenolic extract of N. sativa seed at the dose of 0.3 g/ kg b. w., daily for 18 days. At the end of treatment period, male rats were anesthetized by single injection of thiopental (100 mg/ kg, i.p.), sacrificed, and testes were removed. Samples from testicular tissues were quickly dipped in DEPC solution and frozen at-80 ºC until gene expression levels assessment of inhibin subunits by using semi-quantitative RT-PCR analyses. Male rats that have been treated with phenolic compounds (P group) registered marked increase of all inhibin subunits compared with those treated with methanolic extract, which they were also higher than that of control males. It can be concluded that phenolic compounds of N. sativa seeds have the more efficient role in reproductive functions than methanolic extract of N. sativa seed.
journal of applied pharmaceutical science, 2016
To examine the effect of crude methanolic extract and phenolic compounds obtained from Nigella sa... more To examine the effect of crude methanolic extract and phenolic compounds obtained from Nigella sativa seeds on testicular expression levels of inhibin-α,-βA, and-βB subunits of intact mature male rats, thirty males were randomly assigned to three equal groups (C, M, and P). Males were orally administered with distal water, methanolic extract of N. sativa seed at the dose of 1.0 g/ kg b.w., and phenolic extract of N. sativa seed at the dose of 0.3 g/ kg b. w., daily for 18 days. At the end of treatment period, male rats were anesthetized by single injection of thiopental (100 mg/ kg, i.p.), sacrificed, and testes were removed. Samples from testicular tissues were quickly dipped in DEPC solution and frozen at-80 ºC until gene expression levels assessment of inhibin subunits by using semi-quantitative RT-PCR analyses. Male rats that have been treated with phenolic compounds (P group) registered marked increase of all inhibin subunits compared with those treated with methanolic extract, which they were also higher than that of control males. It can be concluded that phenolic compounds of N. sativa seeds have the more efficient role in reproductive functions than methanolic extract of N. sativa seed.
International journal of health sciences
Current study aimed to investigation the protective effects of zinc and vitamin D in attenuating ... more Current study aimed to investigation the protective effects of zinc and vitamin D in attenuating alveolar response, as anti-inflammatory and antioxidant, in nicotine stressed male rats. Thirty adult males Wistar rats, as a model of mammals, randomly assigned to five equal groups and treatment as follows for 14 days: Control (C) drenched vehicle without treatment, G1 = injected nicotine 1.5 mg/ kg b.w. i/P, G2 administrated orally of zinc 60 mg/ kg b.w., G3 = administrated orally of vitamin D 250 µg/ kg b.w. and G4 administrated orally both of zinc and vitamin D with same doses and stressed by nicotine 1.5 mg/ kg b.w. i/P. At the end of the experiment, rats were anesthetized blood samples have been collected, and then iron, cytokines (IL-6) and malondialdehyde (MDA) and glutathione peroxidase (GPX) were estimation in in serum and lung tissues. The result showed significant (p ≤ 0.5) increase in serum MDA in group G1 when compared with other groups. While significant (p ≤ 0.5) increas...
International journal of health sciences
The current study aimed to investigation the role of zinc and vitamin D in modulating alveolar re... more The current study aimed to investigation the role of zinc and vitamin D in modulating alveolar response of nicotine stress as a model of mammals male Wistar rats. Thirty mature males werekept at 23 ± 2 C°, randomly assigned to five equal groups and treatment for 14 days, C = Control drenched vehicle without treatment, G1 = injected with i/p nicotine 1.5 mg/ kg b.w., G2 = administrated orally of zinc 60 mg/ kgb.w., G3 = administrated orally of vitamin D 250 µg/ kg b.w., G4 = administrated orally both of zinc and vitamin D with same doses stressed and nicotine 1.5 mg/ kgb.w.i/p. Rats were anesthetized with ketamine at the end of the treatment period with xylazine and blood samples have been collected from the optical vein for estimation of serum ferritin and transferrin, then animals were sacrificed and lung was removed and weighted.All groups of rats had lung samples extracted rapidly, dipped in DEPC solution, and frozen under liquid nitrogen for determination of tight junction prote...