Jan Bilski - Academia.edu (original) (raw)

Papers by Jan Bilski

Research paper thumbnail of 199 Adiponectin, the Secretory Hormone of Adipocytes, Prevents the Formation of Ischemia-Reperfusion Gastric Lesions via Anti-Inflammatory and Antioxidative Activity Mediated by cNOS/No System and Sensory Afferent Nerves

Research paper thumbnail of Tu1534: INTESTINAL ALKALINE PHOSPHATASE THERAPY COUNTERACTS THE INTENSIFICATION OF COLITIS IN OBESE MICE SUBJECTED TO FORCED TREADMILL EXERCISE BY SHAPING THE INTESTINAL MICROBIOTA AND ATTENUATING OF PRO-INFLAMMATORY AND OXIDATIVE STRESS BIOMARKERS

Research paper thumbnail of Role of Obesity, Physical Exercise, Adipose Tissue-Skeletal Muscle Crosstalk and Molecular Advances in Barrett’s Esophagus and Esophageal Adenocarcinoma

International Journal of Molecular Sciences

Both obesity and esophageal adenocarcinoma (EAC) rates have increased sharply in the United State... more Both obesity and esophageal adenocarcinoma (EAC) rates have increased sharply in the United States and Western Europe in recent years. EAC is a classic example of obesity-related cancer where the risk of EAC increases with increasing body mass index. Pathologically altered visceral fat in obesity appears to play a key role in this process. Visceral obesity may promote EAC by directly affecting gastroesophageal reflux disease and Barrett’s esophagus (BE), as well as a less reflux-dependent effect, including the release of pro-inflammatory adipokines and insulin resistance. Deregulation of adipokine production, such as the shift to an increased amount of leptin relative to “protective” adiponectin, has been implicated in the pathogenesis of BE and EAC. This review discusses not only the epidemiology and pathophysiology of obesity in BE and EAC, but also molecular alterations at the level of mRNA and proteins associated with these esophageal pathologies and the potential role of adipok...

Research paper thumbnail of The Combination of Intestinal Alkaline Phosphatase Treatment with Moderate Physical Activity Alleviates the Severity of Experimental Colitis in Obese Mice via Modulation of Gut Microbiota, Attenuation of Proinflammatory Cytokines, Oxidative Stress Biomarkers and DNA Oxidative Damage in Colonic Mu...

International Journal of Molecular Sciences, 2022

Inflammatory bowel diseases (IBD) are commonly considered as Crohn’s disease and ulcerative colit... more Inflammatory bowel diseases (IBD) are commonly considered as Crohn’s disease and ulcerative colitis, but the possibility that the alterations in gut microbiota and oxidative stress may affect the course of experimental colitis in obese physically exercising mice treated with the intestinal alkaline phosphatase (IAP) has been little elucidated. Mice fed a high-fat-diet (HFD) or normal diet (ND) for 14 weeks were randomly assigned to exercise on spinning wheels (SW) for 7 weeks and treated with IAP followed by intrarectal administration of TNBS. The disease activity index (DAI), grip muscle strength test, oxidative stress biomarkers (MDA, SOD, GSH), DNA damage (8-OHdG), the plasma levels of cytokines IL-2, IL-6, IL-10, IL-12p70, IL-17a, TNF-α, MCP-1 and leptin were assessed, and the stool composition of the intestinal microbiota was determined by next generation sequencing (NGS). The TNBS-induced colitis was worsened in obese sedentary mice as manifested by severe colonic damage, an i...

Research paper thumbnail of Role of secretin and CCK in the stimulation of pancreatic secretion in conscious dogs. Effects of atropine and somatostatin

International Journal of Pancreatology, 1987

Research paper thumbnail of Effects of peptide YY on dog and rat pancreatic secretion in vivo and in vitro

International Journal of Pancreatology, 1988

Research paper thumbnail of Role of Gut-Adipose-muscle Axis in Beneficial Effect of Voluntary Exercise on Experimental Colitis in Mice Fed a Diet-Induced Obesity. Involvement of Protective Irisin and Proinflammatory Biomarkers Released from Mesenteric Fat and Colonic Mucosa

Research paper thumbnail of The influence of winter swimming on the rheological properties of blood

Clinical hemorheology and microcirculation, 2014

The aim of this study was to analyze the changes in blood rheology resulting from regular winter ... more The aim of this study was to analyze the changes in blood rheology resulting from regular winter swimming. The study was carried out on 12 male winter swimmers. Venous blood for morphological, biochemical and rheological analysis was sampled twice from each winter swimmer - at the beginning of the season and after its completion. There were no significant changes detected in the median values of most blood morphological parameters. The only exception pertained to MCHC which was significantly lower after the season. Winter swimming entailed significant decrease in median elongation index values at shear stress levels of 0.30 Pa and 0.58 Pa, and significant increase in median values of this parameter at shear stress levels ≥1.13 Pa. No significant changes were observed in winter swimmers' median values of aggregation indices and plasma viscosity. The median level of glucose was lower post winter swimming in comparison to the pre-seasonal values. In contrast, one season of winter s...

Research paper thumbnail of The Role of Physical Exercise in Inflammatory Bowel Disease

BioMed Research International, 2014

We reviewed and analyzed the relationship between physical exercise and inflammatory bowel diseas... more We reviewed and analyzed the relationship between physical exercise and inflammatory bowel disease (IBD) which covers a group of chronic, relapsing, and remitting intestinal disorders including Crohn’s disease (CD) and ulcerative colitis. The etiology of IBD likely involves a combination of genetic predisposition and environmental risk factors. Physical training has been suggested to be protective against the onset of IBD, but there are inconsistencies in the findings of the published literature. Hypertrophy of the mesenteric white adipose tissue (mWAT) is recognized as a characteristic feature of CD, but its importance for the perpetuation of onset of this intestinal disease is unknown. Adipocytes synthesize proinflammatory and anti-inflammatory cytokines. Hypertrophy of mWAT could play a role as a barrier to the inflammatory process, but recent data suggest that deregulation of adipokine secretion is involved in the pathogenesis of CD. Adipocytokines and macrophage mediators perpe...

Research paper thumbnail of Effects of a Meal on the Hemorheologic Responses to Exercise in Young Males

BioMed Research International, 2014

Aim. This study investigates the changes in hemorheologic parameters resulting from exercise foll... more Aim. This study investigates the changes in hemorheologic parameters resulting from exercise followed by a standard meal. Methods. In twelve moderately active men a period of exercise on a bicycle ergometer for 30 min at 60% VO2max was followed by a test meal or by 30 min rest. Venous blood was sampled for further analysis at baseline, after exercise, and after the meal/rest period. Results. The elongation index (EI) was reduced and a marked rise in plasma viscosity was observed after exercise. A significant decrease in half time of total aggregation (T1/2) and a rise in aggregation index (AI) after exercise were observed; however, after the postexercise period these changes were reversed. Conclusion. The present study demonstrates that physical exercise causes several changes in blood rheology parameters, such as an increase of blood viscosity, a decrease in EI and an increase in AI, and a fall in the T1/2 values. The meal eaten in the postexercise period caused a further reduction...

Research paper thumbnail of De-Nol stimulates gastric and duodenal alkaline secretion through prostaglandin dependent mechanism

Research paper thumbnail of Leptin protects the pancreas against its damage by overstmulation with caerulein. Role of TNFA and IL-4

Research paper thumbnail of Inhibition of stimulated exocrine pancreatic secretion by leptin

Research paper thumbnail of Effect of sensory nerves and CGRP on development of caerulein-induced pancreatitis and pancreatic regeneration

Research paper thumbnail of Nα-methyl histamine inhibits pancreatic secretion and caerulein-induced pancreatitis in rats

Research paper thumbnail of Calcitonin gene-related peptide (CGRP) can prevent or augment pancreatic damage in caerulein-induced pancreatitis

Research paper thumbnail of The role of oxygen-derived free radicals in acute pancreatitis

Research paper thumbnail of Epidermal growth factor (EGF) limits the pancreatic damage in ischemia/ireperfusion-induced pancreatitis

Research paper thumbnail of Comparison of Somatostatin and Its Highly Potent Hexa- and Octapeptide Analogs on Exocrine and Endocrine Pancreatic Secretion

Experimental Biology and Medicine, 1988

The effects on pancreatic responses of highly potent cyclic hexapeptide (cyclo (N-Me-Ala-Phe-D-Tr... more The effects on pancreatic responses of highly potent cyclic hexapeptide (cyclo (N-Me-Ala-Phe-D-Trp-Lys-Thr-Phe)) (Veber analog) and octapeptide analogs of somatostatin such as D-Phe-Cys-Phe-D-Trp-Lys-Thr-Cys-Thr-ol (SMS 201-995), D-Phe-Cys-Tyr-D-Trp-Lys-Val-Cys-Thr-NH2 (RC-121), and D-Phe-Cys-Tyr-D-Trp-Lys-Val-Cys-Trp-NH2 (RC-160) have been compared with somatostatin tetradecapeptide (SS-14) and atropine. The parameters evaluated were pancreatic responses to secretin and meat feeding in conscious dogs with chronic pancreatic fistula and amylase release from the dispersed pancreatic acini. The analogs were administered intravenously or intraduodenally. The cyclic hexapeptide and octapeptide analogs, given iv in graded doses against a constant background stimulation with secretin, produced similar and dose-dependent inhibition of pancreatic HCO3- and protein secretion. Analogs RC-121, RC-160, and the Veber analog were about two to four times more active than SS-14 in suppressing HCO3- secretion and equipotent in reducing protein secretion, but SMS 201-995 was only about half as potent as somatostatin in inhibiting HCO3-. RC-160 was effective in inhibiting secretin-induced protein secretion at lower doses than other analogs. In tests with feeding, SMS 201-995, the Veber analog, RC-121, and RC-160 were more potent inhibitors of exocrine pancreatic secretion of HCO3- and protein and exhibited more prolonged inhibitory effects than SS-14. The Veber analog, RC-121, and RC-160 were also more effective after intraduodenal administration. Atropine also caused significant inhibition of both HCO3- and protein responses to secretin and meal feeding. All four analogs decreased the postprandial insulin and pancreatic polypeptide release to a similar degree as SS-14. Neither SS-14 nor the analogs tested significantly affected basal or caerulein-, gastrin-, secretin-, or bethanechol-stimulated amylase release from the dispersed canine pancreatic acini. Atropine reduced amylase release induced by bethanechol, but not that stimulated by caerulein, gastrin, or secretin. This indicated that the analogs, as somatostatin, are ineffective as secretory inhibitors in vitro. We conclude that cyclic hexapeptide and octapeptide analogs are more potent and longer acting inhibitors of pancreatic secretion than somatostatin-14 in vivo.

Research paper thumbnail of Role of Endogenous Prostaglandins in Duodenal Alkaline Response to Luminal Hydrochloric Acid or Arachidonic Acid in Conscious Dogs

Digestion, 2009

Duodenal secretion of HCO-3 and luminal release of PGE2 were measured in conscious dogs. The resu... more Duodenal secretion of HCO-3 and luminal release of PGE2 were measured in conscious dogs. The results show that the HCO-3 secretion is closely correlated with the luminal release of PGE2 and that both the HCO-3 and the PGE2 outputs increase dose-dependently after topical application of hydrochloric acid or arachidonic acid. Indomethacin reduced basal HCO-3 and PGE2 release and prevented their increase in response to hydrochloric acid or arachidonic acid. We conclude that mucosal PGE2 plays an important role in the alkaline secretion from the duodenum.

Research paper thumbnail of 199 Adiponectin, the Secretory Hormone of Adipocytes, Prevents the Formation of Ischemia-Reperfusion Gastric Lesions via Anti-Inflammatory and Antioxidative Activity Mediated by cNOS/No System and Sensory Afferent Nerves

Research paper thumbnail of Tu1534: INTESTINAL ALKALINE PHOSPHATASE THERAPY COUNTERACTS THE INTENSIFICATION OF COLITIS IN OBESE MICE SUBJECTED TO FORCED TREADMILL EXERCISE BY SHAPING THE INTESTINAL MICROBIOTA AND ATTENUATING OF PRO-INFLAMMATORY AND OXIDATIVE STRESS BIOMARKERS

Research paper thumbnail of Role of Obesity, Physical Exercise, Adipose Tissue-Skeletal Muscle Crosstalk and Molecular Advances in Barrett’s Esophagus and Esophageal Adenocarcinoma

International Journal of Molecular Sciences

Both obesity and esophageal adenocarcinoma (EAC) rates have increased sharply in the United State... more Both obesity and esophageal adenocarcinoma (EAC) rates have increased sharply in the United States and Western Europe in recent years. EAC is a classic example of obesity-related cancer where the risk of EAC increases with increasing body mass index. Pathologically altered visceral fat in obesity appears to play a key role in this process. Visceral obesity may promote EAC by directly affecting gastroesophageal reflux disease and Barrett’s esophagus (BE), as well as a less reflux-dependent effect, including the release of pro-inflammatory adipokines and insulin resistance. Deregulation of adipokine production, such as the shift to an increased amount of leptin relative to “protective” adiponectin, has been implicated in the pathogenesis of BE and EAC. This review discusses not only the epidemiology and pathophysiology of obesity in BE and EAC, but also molecular alterations at the level of mRNA and proteins associated with these esophageal pathologies and the potential role of adipok...

Research paper thumbnail of The Combination of Intestinal Alkaline Phosphatase Treatment with Moderate Physical Activity Alleviates the Severity of Experimental Colitis in Obese Mice via Modulation of Gut Microbiota, Attenuation of Proinflammatory Cytokines, Oxidative Stress Biomarkers and DNA Oxidative Damage in Colonic Mu...

International Journal of Molecular Sciences, 2022

Inflammatory bowel diseases (IBD) are commonly considered as Crohn’s disease and ulcerative colit... more Inflammatory bowel diseases (IBD) are commonly considered as Crohn’s disease and ulcerative colitis, but the possibility that the alterations in gut microbiota and oxidative stress may affect the course of experimental colitis in obese physically exercising mice treated with the intestinal alkaline phosphatase (IAP) has been little elucidated. Mice fed a high-fat-diet (HFD) or normal diet (ND) for 14 weeks were randomly assigned to exercise on spinning wheels (SW) for 7 weeks and treated with IAP followed by intrarectal administration of TNBS. The disease activity index (DAI), grip muscle strength test, oxidative stress biomarkers (MDA, SOD, GSH), DNA damage (8-OHdG), the plasma levels of cytokines IL-2, IL-6, IL-10, IL-12p70, IL-17a, TNF-α, MCP-1 and leptin were assessed, and the stool composition of the intestinal microbiota was determined by next generation sequencing (NGS). The TNBS-induced colitis was worsened in obese sedentary mice as manifested by severe colonic damage, an i...

Research paper thumbnail of Role of secretin and CCK in the stimulation of pancreatic secretion in conscious dogs. Effects of atropine and somatostatin

International Journal of Pancreatology, 1987

Research paper thumbnail of Effects of peptide YY on dog and rat pancreatic secretion in vivo and in vitro

International Journal of Pancreatology, 1988

Research paper thumbnail of Role of Gut-Adipose-muscle Axis in Beneficial Effect of Voluntary Exercise on Experimental Colitis in Mice Fed a Diet-Induced Obesity. Involvement of Protective Irisin and Proinflammatory Biomarkers Released from Mesenteric Fat and Colonic Mucosa

Research paper thumbnail of The influence of winter swimming on the rheological properties of blood

Clinical hemorheology and microcirculation, 2014

The aim of this study was to analyze the changes in blood rheology resulting from regular winter ... more The aim of this study was to analyze the changes in blood rheology resulting from regular winter swimming. The study was carried out on 12 male winter swimmers. Venous blood for morphological, biochemical and rheological analysis was sampled twice from each winter swimmer - at the beginning of the season and after its completion. There were no significant changes detected in the median values of most blood morphological parameters. The only exception pertained to MCHC which was significantly lower after the season. Winter swimming entailed significant decrease in median elongation index values at shear stress levels of 0.30 Pa and 0.58 Pa, and significant increase in median values of this parameter at shear stress levels ≥1.13 Pa. No significant changes were observed in winter swimmers' median values of aggregation indices and plasma viscosity. The median level of glucose was lower post winter swimming in comparison to the pre-seasonal values. In contrast, one season of winter s...

Research paper thumbnail of The Role of Physical Exercise in Inflammatory Bowel Disease

BioMed Research International, 2014

We reviewed and analyzed the relationship between physical exercise and inflammatory bowel diseas... more We reviewed and analyzed the relationship between physical exercise and inflammatory bowel disease (IBD) which covers a group of chronic, relapsing, and remitting intestinal disorders including Crohn’s disease (CD) and ulcerative colitis. The etiology of IBD likely involves a combination of genetic predisposition and environmental risk factors. Physical training has been suggested to be protective against the onset of IBD, but there are inconsistencies in the findings of the published literature. Hypertrophy of the mesenteric white adipose tissue (mWAT) is recognized as a characteristic feature of CD, but its importance for the perpetuation of onset of this intestinal disease is unknown. Adipocytes synthesize proinflammatory and anti-inflammatory cytokines. Hypertrophy of mWAT could play a role as a barrier to the inflammatory process, but recent data suggest that deregulation of adipokine secretion is involved in the pathogenesis of CD. Adipocytokines and macrophage mediators perpe...

Research paper thumbnail of Effects of a Meal on the Hemorheologic Responses to Exercise in Young Males

BioMed Research International, 2014

Aim. This study investigates the changes in hemorheologic parameters resulting from exercise foll... more Aim. This study investigates the changes in hemorheologic parameters resulting from exercise followed by a standard meal. Methods. In twelve moderately active men a period of exercise on a bicycle ergometer for 30 min at 60% VO2max was followed by a test meal or by 30 min rest. Venous blood was sampled for further analysis at baseline, after exercise, and after the meal/rest period. Results. The elongation index (EI) was reduced and a marked rise in plasma viscosity was observed after exercise. A significant decrease in half time of total aggregation (T1/2) and a rise in aggregation index (AI) after exercise were observed; however, after the postexercise period these changes were reversed. Conclusion. The present study demonstrates that physical exercise causes several changes in blood rheology parameters, such as an increase of blood viscosity, a decrease in EI and an increase in AI, and a fall in the T1/2 values. The meal eaten in the postexercise period caused a further reduction...

Research paper thumbnail of De-Nol stimulates gastric and duodenal alkaline secretion through prostaglandin dependent mechanism

Research paper thumbnail of Leptin protects the pancreas against its damage by overstmulation with caerulein. Role of TNFA and IL-4

Research paper thumbnail of Inhibition of stimulated exocrine pancreatic secretion by leptin

Research paper thumbnail of Effect of sensory nerves and CGRP on development of caerulein-induced pancreatitis and pancreatic regeneration

Research paper thumbnail of Nα-methyl histamine inhibits pancreatic secretion and caerulein-induced pancreatitis in rats

Research paper thumbnail of Calcitonin gene-related peptide (CGRP) can prevent or augment pancreatic damage in caerulein-induced pancreatitis

Research paper thumbnail of The role of oxygen-derived free radicals in acute pancreatitis

Research paper thumbnail of Epidermal growth factor (EGF) limits the pancreatic damage in ischemia/ireperfusion-induced pancreatitis

Research paper thumbnail of Comparison of Somatostatin and Its Highly Potent Hexa- and Octapeptide Analogs on Exocrine and Endocrine Pancreatic Secretion

Experimental Biology and Medicine, 1988

The effects on pancreatic responses of highly potent cyclic hexapeptide (cyclo (N-Me-Ala-Phe-D-Tr... more The effects on pancreatic responses of highly potent cyclic hexapeptide (cyclo (N-Me-Ala-Phe-D-Trp-Lys-Thr-Phe)) (Veber analog) and octapeptide analogs of somatostatin such as D-Phe-Cys-Phe-D-Trp-Lys-Thr-Cys-Thr-ol (SMS 201-995), D-Phe-Cys-Tyr-D-Trp-Lys-Val-Cys-Thr-NH2 (RC-121), and D-Phe-Cys-Tyr-D-Trp-Lys-Val-Cys-Trp-NH2 (RC-160) have been compared with somatostatin tetradecapeptide (SS-14) and atropine. The parameters evaluated were pancreatic responses to secretin and meat feeding in conscious dogs with chronic pancreatic fistula and amylase release from the dispersed pancreatic acini. The analogs were administered intravenously or intraduodenally. The cyclic hexapeptide and octapeptide analogs, given iv in graded doses against a constant background stimulation with secretin, produced similar and dose-dependent inhibition of pancreatic HCO3- and protein secretion. Analogs RC-121, RC-160, and the Veber analog were about two to four times more active than SS-14 in suppressing HCO3- secretion and equipotent in reducing protein secretion, but SMS 201-995 was only about half as potent as somatostatin in inhibiting HCO3-. RC-160 was effective in inhibiting secretin-induced protein secretion at lower doses than other analogs. In tests with feeding, SMS 201-995, the Veber analog, RC-121, and RC-160 were more potent inhibitors of exocrine pancreatic secretion of HCO3- and protein and exhibited more prolonged inhibitory effects than SS-14. The Veber analog, RC-121, and RC-160 were also more effective after intraduodenal administration. Atropine also caused significant inhibition of both HCO3- and protein responses to secretin and meal feeding. All four analogs decreased the postprandial insulin and pancreatic polypeptide release to a similar degree as SS-14. Neither SS-14 nor the analogs tested significantly affected basal or caerulein-, gastrin-, secretin-, or bethanechol-stimulated amylase release from the dispersed canine pancreatic acini. Atropine reduced amylase release induced by bethanechol, but not that stimulated by caerulein, gastrin, or secretin. This indicated that the analogs, as somatostatin, are ineffective as secretory inhibitors in vitro. We conclude that cyclic hexapeptide and octapeptide analogs are more potent and longer acting inhibitors of pancreatic secretion than somatostatin-14 in vivo.

Research paper thumbnail of Role of Endogenous Prostaglandins in Duodenal Alkaline Response to Luminal Hydrochloric Acid or Arachidonic Acid in Conscious Dogs

Digestion, 2009

Duodenal secretion of HCO-3 and luminal release of PGE2 were measured in conscious dogs. The resu... more Duodenal secretion of HCO-3 and luminal release of PGE2 were measured in conscious dogs. The results show that the HCO-3 secretion is closely correlated with the luminal release of PGE2 and that both the HCO-3 and the PGE2 outputs increase dose-dependently after topical application of hydrochloric acid or arachidonic acid. Indomethacin reduced basal HCO-3 and PGE2 release and prevented their increase in response to hydrochloric acid or arachidonic acid. We conclude that mucosal PGE2 plays an important role in the alkaline secretion from the duodenum.