J. Bournat - Academia.edu (original) (raw)
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Maastricht University, Faculty of Health Medicine and Life sciences
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Papers by J. Bournat
Biochemical Society transactions, 1998
Journal of Neuroscience Research, 2000
Expression of the Wnt-1 oncogene in PC12 cells induces morphological and biochemical changes, inc... more Expression of the Wnt-1 oncogene in PC12 cells induces morphological and biochemical changes, including upregulation of cell adhesion and lack of differentiation in response to growth factors. The survival of PC12 cells is known to be mediated in part by phosphatidylinositol-3 kinase (PI-3 kinase)-dependent activation of the transcription factor nuclear factor-B (NF-B). We investigated the effect of Wnt-1 expression on cell survival and NF-B activation using PC12 cells expressing Wnt-1 (PC12/Wnt1) and a reporter vector in which firefly luciferase expression is under the control of NF-B consensus sequences. Serum deprivation caused apoptosis and decreased NF-B activity in wild type PC12 cells. PC12/Wnt-1 cells showed less apoptosis in the absence of serum, and the levels of NF-B activity were higher than in wild type PC12 cells. NF-B activity was also increased by the transient expression of Wnt-1 in PC12 cells and it was completely inhibited in both PC12 and PC12/Wnt-1 cells by a dominant negative mutant IB-␣ that has been shown to prevent NF-B activation. Agents known to inhibit NF-B-induced apoptosis in PC12 as well as in PC12/Wnt-1 cells, indicating a role of NF-B activation in the anti-apoptotic effect of Wnt-1. Inhibition of PI-3 kinase with wortmannin, or with a dominant negative p85 regulatory subunit of the PI-3 kinase, blocked NF-B activity in PC12 cells but caused only partial inhibition in PC12/Wnt-1 cells. The effect of Wnt-1 in activating NF-B can be mimicked by inhibition of glycogen synthase kinase-3 (GSK-3) with lithium or with a dominant negative GSK-3. Our results show that expression of Wnt-1 increases survival of PC12 cells in the absence of serum by activating the anti-apoptotic factor NF-B. Wnt-1-induced activation of NF-B is partially independent of PI-3 kinase and can be mimicked by inhibition of GSK-3. J. Neurosci.
Molecular Brain Research, 2001
The Y1 receptor for neuropeptide Y (NPY-Y1) is constitutively expressed in PC12 cells. In this st... more The Y1 receptor for neuropeptide Y (NPY-Y1) is constitutively expressed in PC12 cells. In this study, we examined the role of nerve growth factor (NGF), pituitary adenylyl cyclase activating polypeptide (PACAP) and dexamethasone on the expression of the gene encoding the rat NPY-Y1 receptor in PC12 cells. A fusion gene (pY1-Luc) was constructed where the reporter enzyme firefly luciferase was placed under the control of 700 bp of the promoter region of the rat NPY-Y1 receptor gene. This promoter region contains recognition consensus sequences for various transcription factors, including one activation protein-1 (AP-1) site, two cyclic AMP responsive element sites, one estrogen receptor element site and four glucocorticoid receptor element sites. NGF increased luciferase activity in a concentration dependent manner. This increase was inhibited by K-252a, a trk A receptor inhibitor, and calphostin C, a PKC inhibitor. PACAP-38 increased luciferase activity in a concentration dependent manner. This activation was inhibited by H-89. Dexamethasone increased transcription of NPY-Y1 gene in PC12 cells. These results indicate that differentiation of PC12 cells into endocrine-like phenotype by dexamethasone and into a neuronal-like phenotype by either NGF or PACAP-38 increases the transcriptional activity of the NPY-Y1 receptor gene in PC12 cells.
Brain, 2011
Abbreviations: CSPGs = chondroitin sulphate proteoglycans; EGFR = epidermal growth factor recepto... more Abbreviations: CSPGs = chondroitin sulphate proteoglycans; EGFR = epidermal growth factor receptor; ErbB = erythroblastic leukaemia viral oncogene homologue; PCR = polymerase chain reaction; PIAS3 = protein inhibitor of activated STAT3; Sema3A = semaphorin 3A; SHP1 = Src homology phosphatase 1; SOCS3 = suppressor of cytokine signalling 3; STAT3 = signal transducer and activator of transcription 3; TACE = tumor necrosis-alpha converting enzyme
Biochemical Society transactions, 1998
Journal of Neuroscience Research, 2000
Expression of the Wnt-1 oncogene in PC12 cells induces morphological and biochemical changes, inc... more Expression of the Wnt-1 oncogene in PC12 cells induces morphological and biochemical changes, including upregulation of cell adhesion and lack of differentiation in response to growth factors. The survival of PC12 cells is known to be mediated in part by phosphatidylinositol-3 kinase (PI-3 kinase)-dependent activation of the transcription factor nuclear factor-B (NF-B). We investigated the effect of Wnt-1 expression on cell survival and NF-B activation using PC12 cells expressing Wnt-1 (PC12/Wnt1) and a reporter vector in which firefly luciferase expression is under the control of NF-B consensus sequences. Serum deprivation caused apoptosis and decreased NF-B activity in wild type PC12 cells. PC12/Wnt-1 cells showed less apoptosis in the absence of serum, and the levels of NF-B activity were higher than in wild type PC12 cells. NF-B activity was also increased by the transient expression of Wnt-1 in PC12 cells and it was completely inhibited in both PC12 and PC12/Wnt-1 cells by a dominant negative mutant IB-␣ that has been shown to prevent NF-B activation. Agents known to inhibit NF-B-induced apoptosis in PC12 as well as in PC12/Wnt-1 cells, indicating a role of NF-B activation in the anti-apoptotic effect of Wnt-1. Inhibition of PI-3 kinase with wortmannin, or with a dominant negative p85 regulatory subunit of the PI-3 kinase, blocked NF-B activity in PC12 cells but caused only partial inhibition in PC12/Wnt-1 cells. The effect of Wnt-1 in activating NF-B can be mimicked by inhibition of glycogen synthase kinase-3 (GSK-3) with lithium or with a dominant negative GSK-3. Our results show that expression of Wnt-1 increases survival of PC12 cells in the absence of serum by activating the anti-apoptotic factor NF-B. Wnt-1-induced activation of NF-B is partially independent of PI-3 kinase and can be mimicked by inhibition of GSK-3. J. Neurosci.
Molecular Brain Research, 2001
The Y1 receptor for neuropeptide Y (NPY-Y1) is constitutively expressed in PC12 cells. In this st... more The Y1 receptor for neuropeptide Y (NPY-Y1) is constitutively expressed in PC12 cells. In this study, we examined the role of nerve growth factor (NGF), pituitary adenylyl cyclase activating polypeptide (PACAP) and dexamethasone on the expression of the gene encoding the rat NPY-Y1 receptor in PC12 cells. A fusion gene (pY1-Luc) was constructed where the reporter enzyme firefly luciferase was placed under the control of 700 bp of the promoter region of the rat NPY-Y1 receptor gene. This promoter region contains recognition consensus sequences for various transcription factors, including one activation protein-1 (AP-1) site, two cyclic AMP responsive element sites, one estrogen receptor element site and four glucocorticoid receptor element sites. NGF increased luciferase activity in a concentration dependent manner. This increase was inhibited by K-252a, a trk A receptor inhibitor, and calphostin C, a PKC inhibitor. PACAP-38 increased luciferase activity in a concentration dependent manner. This activation was inhibited by H-89. Dexamethasone increased transcription of NPY-Y1 gene in PC12 cells. These results indicate that differentiation of PC12 cells into endocrine-like phenotype by dexamethasone and into a neuronal-like phenotype by either NGF or PACAP-38 increases the transcriptional activity of the NPY-Y1 receptor gene in PC12 cells.
Brain, 2011
Abbreviations: CSPGs = chondroitin sulphate proteoglycans; EGFR = epidermal growth factor recepto... more Abbreviations: CSPGs = chondroitin sulphate proteoglycans; EGFR = epidermal growth factor receptor; ErbB = erythroblastic leukaemia viral oncogene homologue; PCR = polymerase chain reaction; PIAS3 = protein inhibitor of activated STAT3; Sema3A = semaphorin 3A; SHP1 = Src homology phosphatase 1; SOCS3 = suppressor of cytokine signalling 3; STAT3 = signal transducer and activator of transcription 3; TACE = tumor necrosis-alpha converting enzyme