JESSICA REY - Academia.edu (original) (raw)
Papers by JESSICA REY
Deleted Journal, Mar 1, 2023
Scientific reports, Mar 1, 2024
Calophyllum soulattri Burm.f. is traditionally used to treat skin infections and reduce rheumatic... more Calophyllum soulattri Burm.f. is traditionally used to treat skin infections and reduce rheumatic pain, yet genetic and genomic studies are still limited. Here, we present the first complete mitochondrial genome of C. soulattri. It is 378,262 bp long with 43.97% GC content, containing 55 genes (30 proteincoding, 5 rRNA, and 20 tRNA). Repeat analysis of the mitochondrial genome revealed 194 SSRs, mostly mononucleotides, and 266 pairs of dispersed repeats ( ≥ 30 bp) that were predominantly palindromic. There were 23 homologous fragments found between the mitochondrial and plastome genomes. We also predicted 345 C-to-U RNA editing sites from 30 protein-coding genes (PCGs) of the C. soulatrii mitochondrial genome. These RNA editing events created the start codon of nad1 and the stop codon of ccmFc. Most PCGs of the C. soulattri mitochondrial genome underwent negative selection, but atp4 and ccmB experienced positive selection. Phylogenetic analyses showed C. soulattri is a sister taxon of Garcinia mangostana. This study has shed light on C. soulattri's evolution and Malpighiales' phylogeny. As the first complete mitochondrial genome in Calophyllaceae, it can be used as a reference genome for other medicinal plant species within the family for future genetic studies. Calophyllum soulattri Burm.f. is a medicinally important evergreen tree belonging to the Calophyllaceae family of the order Malpighiales 1 . The natural distribution of this species extends from South Indo-China to the Caroline Islands (. plant softh eworl donli ne. org/). Its leaves, roots, and stem bark have been shown to exhibit antimicrobial, cytotoxic, and anti-obesity properties 2,3 . In C. soulattri, the most active secondary metabolites were calosubellinone, garsubellin B, and soulattrin 4,5 . Generally, the genus Calophyllum contains a diverse array of bioactive constituents, including xanthones and coumarins 3 . Cellular respiration is a complex metabolic process involving the breakdown of sugar molecules in the presence of oxygen to generate adenosine triphosphate (ATP) which occurs in mitochondria . Apart from their primary function in ATP production, mitochondria actively participate in several metabolic pathways. Notably, they are involved in the biosynthesis of specific amino acids, lipids, and heme-a crucial component of various enzymatic systems 8 . Moreover, mitochondria are thought to have originated from an ancient endosymbiotic event, wherein a free-living bacterium is engulfed by a eukaryotic cell 9 . This endosymbiotic theory accounts for the presence of a double membrane in mitochondria, along with their distinct circular DNA, resembling that of bacterial DNA 10 . The plant mitochondrial genome typically consists of a circular DNA molecule, with sizes ranging from approximately 66 kb 11 to about 11.7 Mb 12 among the genomes sequenced to date. Nonetheless, subgenomic forms also exist because of repeat-mediated homologous recombination 13 . Conversely, plastomes have a more consistent regular structure characterized by a highly conserved arrangement of genes in a circular DNA molecule 14 . This is why the focus has predominantly been on plastomes in the realm of plant phylogenetic studies. In addition, the chloroplast genome of C. soulattri has been recently reported 15 . To date, the number of chloroplast genomes deposited in NCBI GenBank (. ncbi. nlm. nih. gov/ genome/ browse# !/ organ elles/) is more than 9,000; along with more than 1,000 plastid genomes. In contrast, only more than 540 plant mitochondrial genomes are available in the database. However, no mitochondrial genome from the Calophyllaceae family has been included in the NCBI GenBank Organelle Genome repository. But several mitogenome studies have already been reported in the order Malpighiales such in Salix species, Bruguiera sexangula, Passiflora edulis, Populus species, and Garcinia mangostana . The differences in the number of organelle genomes can be explained by the difficulties encountered during the assembly process. Mitochondrial genomes often possess repetitive regions and frequently undergo rearrangement events, which present challenges when traditional short-read sequencing methods are used for their assembly. Therefore, the process of obtaining complete and accurate mitochondrial genome sequences is more intricate than that of plastomes and often requires the application of
SABRAO Journal of Breeding and Genetics
Strawberry (Fragaria x ananassa Duchesne ex Rozier) is an economically important fruit crop grown... more Strawberry (Fragaria x ananassa Duchesne ex Rozier) is an economically important fruit crop grown commercially worldwide due to its known nutritional value. The demand for strawberries in the Philippines has been increasing but varietal development is slow due to the lack of genetic information on locally available germplasm. In addition, molecular marker resources for strawberries are limited. Here, we developed Simple Sequence Repeats (SSR) markers to characterize strawberries cultivated in tropical environments, particularly in the Philippines. In silico mining generated a total of 219,239 SSR markers, from which 160,025 unique markers were identified. Polymorphism scoring and the variation on the expected alleles resulting to the synthesis of the top 160 markers were validated using representative strawberry cultivars from the core collection. All 70 markers produced successful amplicons from all the genotypes, but only 67 markers displayed polymorphism. Using UPGMA, genetic sim...
Molecular Breeding, 2012
Breeding of multi-stress tolerant rice varieties with higher grain yields is the best option to e... more Breeding of multi-stress tolerant rice varieties with higher grain yields is the best option to enhance the rice productivity of abiotic stresses prone areas. It also poses the greatest challenge to plant breeders to breed rice varieties for such stress prone conditions. Here, we carried out a designed QTL pyramiding experiment to develop high yielding "Green Super Rice" varieties with significantly improved tolerance to salt stress and grain yield. Using the F 4 population derived from a cross between two selected introgression lines, we were able to develop six mostly homozygous promising high yielding lines with significantly improved salt tolerance and grain yield under optimal and/or saline conditions in 3 years. Simultaneous mapping using the same breeding population and tunable genotyping-by-sequencing technology, we identified three QTL affecting salt injury score and leaf chlorophyll content. By analyzing 32M SNP data of the grandparents and graphical genotypes of the parents, we discovered 87 positional candidate genes for salt tolerant QTL. According to their functional annotation, we inferred the most likely candidate genes. We demonstrated that designed QTL pyramiding is a powerful strategy for simultaneous improvement and genetic dissection of complex traits in rice.
Philippine Journal of Science
Cavendish banana is one of the most consumed fruits across countries with demands expected to gro... more Cavendish banana is one of the most consumed fruits across countries with demands expected to grow by 2026. The Philippines is one of the top producers of Cavendish bananas, yet is negatively impacted by the Sigatoka disease complex (SDC) caused by three Pseudocercospora spp. – namely, P. musae, P. fijiensis, and P. eumusae. These pathogens are persistent in the field despite various control programs implemented in plantations. Whole genomes of fungi have aided in understanding the interactions of co-occurring pathogens and the emergence of fungicide-resistant populations. This study sought to characterize the underlying basis of Pseudocercospora spp. co-infection by determining possible processes involved during disease establishment and factors associated with fungicide resistance development through an in silico approach. Homology search on disease establishment-related proteins revealed that Pseudocercospora spp. share more similar than unique proteins, suggesting an independent...
Scientific Reports, 2016
More than two billion people are micronutrient deficient. Polished grains of popular rice varieti... more More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 μg g −1 iron (Fe) and 16 μg g −1 zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 μg g −1 Fe and 28 μg g −1 Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 μg g −1 in glasshouse settings; in contrast, under field conditions, 4 μg g −1 was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 μg g −1 Fe and 45.7 μg g −1 Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase (OsNAS2) and soybean ferritin (SferH-1) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds. Micronutrient deficiencies or "hidden hunger" affect about 38% of pregnant women and 43% of preschool children worldwide and are most prevalent in developing countries 1. More than 30% of the world's population is anemic 2. Global studies estimate that approximately half of this is due to iron-deficiency anemia (IDA) 3. IDA can affect productivity and cause serious health consequences, including impaired cognitive development in children, a weakened immune system, and increased risk of morbidity 4. Zinc deficiency is a major cause of stunting among children 5. About 165 million children with stunted growth run a risk of compromised cognitive development and physical capability 6,7. Biofortification, the delivery of micronutrients via staple food crops, has been proposed to complement existing efforts for the alleviation of micronutrient deficiency 8. Conventional breeding efforts for developing Fe-enriched polished rice face major challenges because of the limited variability in Fe concentration in polished grains among rice germplasm 9. Engineering Fe-and Zn-biofortified rice has been extensively reviewed 10,11. However, 15 years after the pioneering attempt 12 and
Zinc (Zn) biofortification breeding lines show different grain Zn contents in various environment... more Zinc (Zn) biofortification breeding lines show different grain Zn contents in various environments and in response to Zn fertilizer application, but it is not clear which environmental variables are most important in explaining this. An experiment was conducted in four contrasting sites in the Philippines: IRRI (Zn-depleted, pH 6.6), Bay (low Zn, high organic matter, pH 6.3), Nueva Ecija (Zn-sufficient, pH 4.5) and Bukidnon (continuously submerged, high iron content, pH 4.8). Twenty-one genotypes were grown on each site with three replicates; the Zn fertilizer treatments were no added Zn and 10 kg Zn ha-1 as zinc sulfate, applied at panicle initiation. Water management status was monitored, and grain yield and grain Zn concentration were analyzed. The data show that the IRRI site had the highest average grain yield (7 t ha-1) compared with the other sites. Popular varieties such as IR64 and PSBRc 82 produced 4–7 t ha-1, whereas local check variety NSIC 158 produced 5–8 t ha-1. Grain...
In this study, the main subject is the role of the Communications and Internet Revolution in Inte... more In this study, the main subject is the role of the Communications and Internet Revolution in International Relations. The principal motivation of the author to conduct this essay is the need to develop a conceptual and theoretical examination on communications and internet revolution in International Relations. That need is produced by certain technological and structural developments in human life and their effects on the classical understanding of the international relations. The revolutionary developments in communications and internet technology as significant drivers of globalization process, transformation of the communications and information technology into a core of a new world information economy have come into question as globalization proceeds and with the communications and internet revolution we need to understand why global networking has been grown as it did. The main inquiry in this study is whether the rise of global communications has consequences that fundamentally alter the practice of international relations. In order to provide a comprehensive response, the Communications and Internet revolution, the social network and increasing velocity of information must be analyzed clearly.
Accurate pedigree information is critical to animal breeding systems to ensure the highest rate o... more Accurate pedigree information is critical to animal breeding systems to ensure the highest rate of genetic gain and management of inbreeding. The abundance of available genomic data, together with development of high throughput genotyping platforms, means that single nucleotide polymorphisms (SNPs) are now the DNA marker of choice for genomic selection studies. Furthermore the superior qualities of SNPs compared to microsatellite markers allows for standardization between laboratories; a property that is crucial for developing an international set of markers for traceability studies. The objective of this study was to develop a high throughput SNP assay for use in the New Zealand sheep industry that gives accurate pedigree assignment and will allow a reduction in breeder input over lambing. This required two phases of development-firstly, a method of extracting quality DNA from ear-punch tissue performed in a high throughput cost efficient manner and secondly a SNP assay that has the ability to assign paternity to progeny resulting from mob mating. A likelihood based approach to infer paternity was used where sires with the highest LOD score (log of the ratio of the likelihood given parentage to likelihood given non-parentage) are assigned. An 84 ''parentage SNP panel'' was developed that assigned, on average, 99% of progeny to a sire in a problem where there were 3,000 progeny from 120 mob mated sires that included numerous half sib sires. In only 6% of those cases was there another sire with at least a 0.02 probability of paternity. Furthermore dam information (either recorded, or by genotyping possible dams) was absent, highlighting the SNP test's suitability for paternity testing. Utilization of this parentage SNP assay will allow implementation of progeny testing into large commercial farms where the improved accuracy of sire assignment and genetic evaluations will increase genetic gain in the sheep industry.
Deleted Journal, Mar 1, 2023
Scientific reports, Mar 1, 2024
Calophyllum soulattri Burm.f. is traditionally used to treat skin infections and reduce rheumatic... more Calophyllum soulattri Burm.f. is traditionally used to treat skin infections and reduce rheumatic pain, yet genetic and genomic studies are still limited. Here, we present the first complete mitochondrial genome of C. soulattri. It is 378,262 bp long with 43.97% GC content, containing 55 genes (30 proteincoding, 5 rRNA, and 20 tRNA). Repeat analysis of the mitochondrial genome revealed 194 SSRs, mostly mononucleotides, and 266 pairs of dispersed repeats ( ≥ 30 bp) that were predominantly palindromic. There were 23 homologous fragments found between the mitochondrial and plastome genomes. We also predicted 345 C-to-U RNA editing sites from 30 protein-coding genes (PCGs) of the C. soulatrii mitochondrial genome. These RNA editing events created the start codon of nad1 and the stop codon of ccmFc. Most PCGs of the C. soulattri mitochondrial genome underwent negative selection, but atp4 and ccmB experienced positive selection. Phylogenetic analyses showed C. soulattri is a sister taxon of Garcinia mangostana. This study has shed light on C. soulattri's evolution and Malpighiales' phylogeny. As the first complete mitochondrial genome in Calophyllaceae, it can be used as a reference genome for other medicinal plant species within the family for future genetic studies. Calophyllum soulattri Burm.f. is a medicinally important evergreen tree belonging to the Calophyllaceae family of the order Malpighiales 1 . The natural distribution of this species extends from South Indo-China to the Caroline Islands (. plant softh eworl donli ne. org/). Its leaves, roots, and stem bark have been shown to exhibit antimicrobial, cytotoxic, and anti-obesity properties 2,3 . In C. soulattri, the most active secondary metabolites were calosubellinone, garsubellin B, and soulattrin 4,5 . Generally, the genus Calophyllum contains a diverse array of bioactive constituents, including xanthones and coumarins 3 . Cellular respiration is a complex metabolic process involving the breakdown of sugar molecules in the presence of oxygen to generate adenosine triphosphate (ATP) which occurs in mitochondria . Apart from their primary function in ATP production, mitochondria actively participate in several metabolic pathways. Notably, they are involved in the biosynthesis of specific amino acids, lipids, and heme-a crucial component of various enzymatic systems 8 . Moreover, mitochondria are thought to have originated from an ancient endosymbiotic event, wherein a free-living bacterium is engulfed by a eukaryotic cell 9 . This endosymbiotic theory accounts for the presence of a double membrane in mitochondria, along with their distinct circular DNA, resembling that of bacterial DNA 10 . The plant mitochondrial genome typically consists of a circular DNA molecule, with sizes ranging from approximately 66 kb 11 to about 11.7 Mb 12 among the genomes sequenced to date. Nonetheless, subgenomic forms also exist because of repeat-mediated homologous recombination 13 . Conversely, plastomes have a more consistent regular structure characterized by a highly conserved arrangement of genes in a circular DNA molecule 14 . This is why the focus has predominantly been on plastomes in the realm of plant phylogenetic studies. In addition, the chloroplast genome of C. soulattri has been recently reported 15 . To date, the number of chloroplast genomes deposited in NCBI GenBank (. ncbi. nlm. nih. gov/ genome/ browse# !/ organ elles/) is more than 9,000; along with more than 1,000 plastid genomes. In contrast, only more than 540 plant mitochondrial genomes are available in the database. However, no mitochondrial genome from the Calophyllaceae family has been included in the NCBI GenBank Organelle Genome repository. But several mitogenome studies have already been reported in the order Malpighiales such in Salix species, Bruguiera sexangula, Passiflora edulis, Populus species, and Garcinia mangostana . The differences in the number of organelle genomes can be explained by the difficulties encountered during the assembly process. Mitochondrial genomes often possess repetitive regions and frequently undergo rearrangement events, which present challenges when traditional short-read sequencing methods are used for their assembly. Therefore, the process of obtaining complete and accurate mitochondrial genome sequences is more intricate than that of plastomes and often requires the application of
SABRAO Journal of Breeding and Genetics
Strawberry (Fragaria x ananassa Duchesne ex Rozier) is an economically important fruit crop grown... more Strawberry (Fragaria x ananassa Duchesne ex Rozier) is an economically important fruit crop grown commercially worldwide due to its known nutritional value. The demand for strawberries in the Philippines has been increasing but varietal development is slow due to the lack of genetic information on locally available germplasm. In addition, molecular marker resources for strawberries are limited. Here, we developed Simple Sequence Repeats (SSR) markers to characterize strawberries cultivated in tropical environments, particularly in the Philippines. In silico mining generated a total of 219,239 SSR markers, from which 160,025 unique markers were identified. Polymorphism scoring and the variation on the expected alleles resulting to the synthesis of the top 160 markers were validated using representative strawberry cultivars from the core collection. All 70 markers produced successful amplicons from all the genotypes, but only 67 markers displayed polymorphism. Using UPGMA, genetic sim...
Molecular Breeding, 2012
Breeding of multi-stress tolerant rice varieties with higher grain yields is the best option to e... more Breeding of multi-stress tolerant rice varieties with higher grain yields is the best option to enhance the rice productivity of abiotic stresses prone areas. It also poses the greatest challenge to plant breeders to breed rice varieties for such stress prone conditions. Here, we carried out a designed QTL pyramiding experiment to develop high yielding "Green Super Rice" varieties with significantly improved tolerance to salt stress and grain yield. Using the F 4 population derived from a cross between two selected introgression lines, we were able to develop six mostly homozygous promising high yielding lines with significantly improved salt tolerance and grain yield under optimal and/or saline conditions in 3 years. Simultaneous mapping using the same breeding population and tunable genotyping-by-sequencing technology, we identified three QTL affecting salt injury score and leaf chlorophyll content. By analyzing 32M SNP data of the grandparents and graphical genotypes of the parents, we discovered 87 positional candidate genes for salt tolerant QTL. According to their functional annotation, we inferred the most likely candidate genes. We demonstrated that designed QTL pyramiding is a powerful strategy for simultaneous improvement and genetic dissection of complex traits in rice.
Philippine Journal of Science
Cavendish banana is one of the most consumed fruits across countries with demands expected to gro... more Cavendish banana is one of the most consumed fruits across countries with demands expected to grow by 2026. The Philippines is one of the top producers of Cavendish bananas, yet is negatively impacted by the Sigatoka disease complex (SDC) caused by three Pseudocercospora spp. – namely, P. musae, P. fijiensis, and P. eumusae. These pathogens are persistent in the field despite various control programs implemented in plantations. Whole genomes of fungi have aided in understanding the interactions of co-occurring pathogens and the emergence of fungicide-resistant populations. This study sought to characterize the underlying basis of Pseudocercospora spp. co-infection by determining possible processes involved during disease establishment and factors associated with fungicide resistance development through an in silico approach. Homology search on disease establishment-related proteins revealed that Pseudocercospora spp. share more similar than unique proteins, suggesting an independent...
Scientific Reports, 2016
More than two billion people are micronutrient deficient. Polished grains of popular rice varieti... more More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 μg g −1 iron (Fe) and 16 μg g −1 zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 μg g −1 Fe and 28 μg g −1 Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 μg g −1 in glasshouse settings; in contrast, under field conditions, 4 μg g −1 was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 μg g −1 Fe and 45.7 μg g −1 Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase (OsNAS2) and soybean ferritin (SferH-1) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds. Micronutrient deficiencies or "hidden hunger" affect about 38% of pregnant women and 43% of preschool children worldwide and are most prevalent in developing countries 1. More than 30% of the world's population is anemic 2. Global studies estimate that approximately half of this is due to iron-deficiency anemia (IDA) 3. IDA can affect productivity and cause serious health consequences, including impaired cognitive development in children, a weakened immune system, and increased risk of morbidity 4. Zinc deficiency is a major cause of stunting among children 5. About 165 million children with stunted growth run a risk of compromised cognitive development and physical capability 6,7. Biofortification, the delivery of micronutrients via staple food crops, has been proposed to complement existing efforts for the alleviation of micronutrient deficiency 8. Conventional breeding efforts for developing Fe-enriched polished rice face major challenges because of the limited variability in Fe concentration in polished grains among rice germplasm 9. Engineering Fe-and Zn-biofortified rice has been extensively reviewed 10,11. However, 15 years after the pioneering attempt 12 and
Zinc (Zn) biofortification breeding lines show different grain Zn contents in various environment... more Zinc (Zn) biofortification breeding lines show different grain Zn contents in various environments and in response to Zn fertilizer application, but it is not clear which environmental variables are most important in explaining this. An experiment was conducted in four contrasting sites in the Philippines: IRRI (Zn-depleted, pH 6.6), Bay (low Zn, high organic matter, pH 6.3), Nueva Ecija (Zn-sufficient, pH 4.5) and Bukidnon (continuously submerged, high iron content, pH 4.8). Twenty-one genotypes were grown on each site with three replicates; the Zn fertilizer treatments were no added Zn and 10 kg Zn ha-1 as zinc sulfate, applied at panicle initiation. Water management status was monitored, and grain yield and grain Zn concentration were analyzed. The data show that the IRRI site had the highest average grain yield (7 t ha-1) compared with the other sites. Popular varieties such as IR64 and PSBRc 82 produced 4–7 t ha-1, whereas local check variety NSIC 158 produced 5–8 t ha-1. Grain...
In this study, the main subject is the role of the Communications and Internet Revolution in Inte... more In this study, the main subject is the role of the Communications and Internet Revolution in International Relations. The principal motivation of the author to conduct this essay is the need to develop a conceptual and theoretical examination on communications and internet revolution in International Relations. That need is produced by certain technological and structural developments in human life and their effects on the classical understanding of the international relations. The revolutionary developments in communications and internet technology as significant drivers of globalization process, transformation of the communications and information technology into a core of a new world information economy have come into question as globalization proceeds and with the communications and internet revolution we need to understand why global networking has been grown as it did. The main inquiry in this study is whether the rise of global communications has consequences that fundamentally alter the practice of international relations. In order to provide a comprehensive response, the Communications and Internet revolution, the social network and increasing velocity of information must be analyzed clearly.
Accurate pedigree information is critical to animal breeding systems to ensure the highest rate o... more Accurate pedigree information is critical to animal breeding systems to ensure the highest rate of genetic gain and management of inbreeding. The abundance of available genomic data, together with development of high throughput genotyping platforms, means that single nucleotide polymorphisms (SNPs) are now the DNA marker of choice for genomic selection studies. Furthermore the superior qualities of SNPs compared to microsatellite markers allows for standardization between laboratories; a property that is crucial for developing an international set of markers for traceability studies. The objective of this study was to develop a high throughput SNP assay for use in the New Zealand sheep industry that gives accurate pedigree assignment and will allow a reduction in breeder input over lambing. This required two phases of development-firstly, a method of extracting quality DNA from ear-punch tissue performed in a high throughput cost efficient manner and secondly a SNP assay that has the ability to assign paternity to progeny resulting from mob mating. A likelihood based approach to infer paternity was used where sires with the highest LOD score (log of the ratio of the likelihood given parentage to likelihood given non-parentage) are assigned. An 84 ''parentage SNP panel'' was developed that assigned, on average, 99% of progeny to a sire in a problem where there were 3,000 progeny from 120 mob mated sires that included numerous half sib sires. In only 6% of those cases was there another sire with at least a 0.02 probability of paternity. Furthermore dam information (either recorded, or by genotyping possible dams) was absent, highlighting the SNP test's suitability for paternity testing. Utilization of this parentage SNP assay will allow implementation of progeny testing into large commercial farms where the improved accuracy of sire assignment and genetic evaluations will increase genetic gain in the sheep industry.