J. Rojo - Academia.edu (original) (raw)

Papers by J. Rojo

Research paper thumbnail of Impact of Molecular ABO Typing in Case of Serologic Discrepancies in Myeloid Malignancies – a Case Report

Transfusion Medicine and Hemotherapy, 2004

ABSTRACT Background: A case with discrepancies between ABO antigens on red blood cells (RBCs) and... more ABSTRACT Background: A case with discrepancies between ABO antigens on red blood cells (RBCs) and isoagglutinins in the serum of a 49-year-old woman suffering from acute myeloid leukaemia is described. The blood group was typed as A D neg in a previous pregnancy. Material and Methods: 2 monoclonal anti-A, -B and -AB reagents of different manufacturers using different techniques (NaCl suspension in seroplates and in gel columns) were applied for the detection of the patient’s ABO blood group. Suspensions of commercial test erythrocytes (A1, A2, B, AB) were incubated with the patient’s serum for isoagglutinin detection. Results: The antigen A was serologically undetectable. Apart from very weak reactions with B RBCs, isoagglutinins were not detectable even if sensitive methods (tube-spin method) were used. These discrepancies between direct and indirect antigen determinants led to molecular genetic blood group typing based on PCR-SSP. By this method the blood group was typed as A D neg. This result confirmed the previous blood group typing and the serological result obtained after complete remission. Conclusion: Despite excellent monoclonal reagents for the detection of the ABO blood group, the complementary reactions of the isoagglutinins must be observed carefully. In case of serological discrepancies molecular blood group typing should be performed.

Research paper thumbnail of Epidemiology, etiology and diagnosis of venous thrombosis

European journal of medical research, Jan 30, 2004

Venous thromboembolism (VTE) is one of the most frequent multifactorial diseases. It manifests cl... more Venous thromboembolism (VTE) is one of the most frequent multifactorial diseases. It manifests clinically by deep vein thrombosis (DVT) and pulmonary embolism (PE) leading to death in about 6%. It is important to emphasize, that 50% of the patients do not present any symptoms. The prevalence is influenced by age and ethnics. Both, hereditary (Factor V Leiden, G20210A prothrombin gene mutation, deficiencies of protein C, S or antithrombin) and acquired risk factors (estrogen replacement, cancer, cardiovascular disease, surgery, trauma, immobility, use of central venous catheters, autoimmune disease such as anti-phospholipid syndrome) contribute to VTE. The risk increases dramatically by the addition of hyperhomocysteinemia or the combination of several risk factors. Since VTE is a dynamic process able to manifest clinically or to resolve completely, the identification of persons at increased risk is mainly important for early diagnosis and treatment. The diagnostic strategy including...

Research paper thumbnail of Impact of Molecular ABO Typing in Case of Serologic Discrepancies in Myeloid Malignancies – a Case Report

Transfusion Medicine and Hemotherapy, 2004

ABSTRACT Background: A case with discrepancies between ABO antigens on red blood cells (RBCs) and... more ABSTRACT Background: A case with discrepancies between ABO antigens on red blood cells (RBCs) and isoagglutinins in the serum of a 49-year-old woman suffering from acute myeloid leukaemia is described. The blood group was typed as A D neg in a previous pregnancy. Material and Methods: 2 monoclonal anti-A, -B and -AB reagents of different manufacturers using different techniques (NaCl suspension in seroplates and in gel columns) were applied for the detection of the patient’s ABO blood group. Suspensions of commercial test erythrocytes (A1, A2, B, AB) were incubated with the patient’s serum for isoagglutinin detection. Results: The antigen A was serologically undetectable. Apart from very weak reactions with B RBCs, isoagglutinins were not detectable even if sensitive methods (tube-spin method) were used. These discrepancies between direct and indirect antigen determinants led to molecular genetic blood group typing based on PCR-SSP. By this method the blood group was typed as A D neg. This result confirmed the previous blood group typing and the serological result obtained after complete remission. Conclusion: Despite excellent monoclonal reagents for the detection of the ABO blood group, the complementary reactions of the isoagglutinins must be observed carefully. In case of serological discrepancies molecular blood group typing should be performed.

Research paper thumbnail of Epidemiology, etiology and diagnosis of venous thrombosis

European journal of medical research, Jan 30, 2004

Venous thromboembolism (VTE) is one of the most frequent multifactorial diseases. It manifests cl... more Venous thromboembolism (VTE) is one of the most frequent multifactorial diseases. It manifests clinically by deep vein thrombosis (DVT) and pulmonary embolism (PE) leading to death in about 6%. It is important to emphasize, that 50% of the patients do not present any symptoms. The prevalence is influenced by age and ethnics. Both, hereditary (Factor V Leiden, G20210A prothrombin gene mutation, deficiencies of protein C, S or antithrombin) and acquired risk factors (estrogen replacement, cancer, cardiovascular disease, surgery, trauma, immobility, use of central venous catheters, autoimmune disease such as anti-phospholipid syndrome) contribute to VTE. The risk increases dramatically by the addition of hyperhomocysteinemia or the combination of several risk factors. Since VTE is a dynamic process able to manifest clinically or to resolve completely, the identification of persons at increased risk is mainly important for early diagnosis and treatment. The diagnostic strategy including...