Jack frost - Academia.edu (original) (raw)
Papers by Jack frost
My mother's hand, a cursive trained by nuns, wrote, "Found downstairs you can discard,&q... more My mother's hand, a cursive trained by nuns, wrote, "Found downstairs you can discard," across the packet of her father's death: obit, vital certificate, little filler from the Mirror's back pages flint phrases yanked from a trough of stiff-brimmed prose and the doleful tones of medical examiners. No word of wife or child, just that he fell six flights from his perch on a scaffold. Rain brings out a roofer's best and worst and my mother once drew for us the pitch of the sugar refinery's tall boiler house, where a shining statue of Jack Frost pranced the sky in a pointy hat, tossing handfuls of pure granules from a pouch. Should I describe my grandmother's life, left with eight kids? Instead I'll end with the snow-white sprite who did not become "a victim of the weather," as the tabloid's squib portrayed this immigrant leaving his beginnings near Rome only to land in a railroad car of pig iron in Edgewater, N.J., a town consigned to...
Gift of Dr. Mary Jane Esplen.Piano vocal [instrumentation]G major [key]Allegretto [tempo]Popular ... more Gift of Dr. Mary Jane Esplen.Piano vocal [instrumentation]G major [key]Allegretto [tempo]Popular song ; ragtime [form/genre]Men, dog [illustration]Publisher's advertisement on back cover [note
Journal of Immunotoxicology, 2021
Nickel (Ni) in ambient air may vary regionally with contributions from both natural processes and... more Nickel (Ni) in ambient air may vary regionally with contributions from both natural processes and anthropogenic activities. Exposure to Ni compounds in ambient air above a certain level is associated with acute adverse effects, such as upper respiratory tract irritation, pneumonitis, and chronic adverse effects, such as respiratory cancer. Inhalation reference exposure standards are enacted in different jurisdictions to minimize exposures to ambient Ni above levels that can elicit adverse effects. This paper reports a guideline-/GLP-compliant study designed for setting inhalation exposure standards to protect from immunological effects associated with acute exposure to Ni. Female CD-1 mice were exposed via whole-body inhalation to aerosolized nickel chloride hexahydrate for 24-hr at nominal (vs. mean analyzed) concentrations of 20 (16), 50 (44) and 100 (81) mg Ni/m 3. Host T-cell antibody immunological responses to intravenously-injected sheep red blood cells were then measured ex vivo in an Antibody-Forming Cell (AFC) assay. Exposure to the Ni substance significantly decreased spleen cell levels by 33%, but this was within biological variability for outbred mice. No concurrent decreases in spleen, thymus, or body weights were noted. No immunosuppression was observed with the Ni substance in the context of Total Spleen Activity [IgM AFC/spleen (Â 10 3)] and Specific Activity [IgM AFC/spleen cells (Â 10 6)]. Significant concentration-independent increases in Total Spleen Activity and Specific Activity seen with the nickel chloride hexahydrate were normal and within biological variability for outbred mice. In contrast, cyclophosphamide (positive control) significantly decreased spleen cell numbers, spleen and thymus weights, and abolished Specific Activity and Total Spleen Activity. Based on results here, an NOAEC of 81 mg Ni/m 3 for immunosuppressive effects from inhaled nickel chloride hexahydrate was identified. It is hoped this value can be used to derive a reference standard for human exposure to ambient Ni.
Nature, 1998
Dehydroquinate synthase (DHQS) has long been regarded as a catalytic marvel because of its abilit... more Dehydroquinate synthase (DHQS) has long been regarded as a catalytic marvel because of its ability to perform several consecutive chemical reactions in one active site. There has been considerable debate as to whether DHQS is actively involved in all these steps, or whether several steps occur spontaneously, making DHQS a spectator in its own mechanism. DHQS performs the second step in the shikimate pathway, which is required for the synthesis of aromatic compounds in bacteria, microbial eukaryotes and plants. This enzyme is a potential target for new antifungal and antibacterial drugs as the shikimate pathway is absent from mammals and DHQS is required for pathogen virulence. Here we report the crystal structure of DHQS, which has several unexpected features, including a previously unobserved mode for NAD+-binding and an active-site organization that is surprisingly similar to that of alcohol dehydrogenase, in a new protein fold. The structure reveals interactions between the active site and a substrate-analogue inhibitor, which indicate how DHQS can perform multistep catalysis without the formation of unwanted by-products.
Journal of Aerosol Science, 2016
Dosimetric models are essential tools to refine inhalation risk assessments based on local respir... more Dosimetric models are essential tools to refine inhalation risk assessments based on local respiratory effects. Dosimetric adjustments to account for differences in aerosol particle size and respiratory tract deposition and/or clearance among rodents, workers, and the general public can be applied to experimentally-and epidemiologically-determined points of departure (PODs) to calculate size-selected (e.g., PM 10 , inhalable aerosol fraction, respirable aerosol fraction) equivalent concentrations (e.g., HEC or Human Equivalent Concentration; REC or Rodent Equivalent Concentration). A modified POD (e.g., HEC) can then feed into existing frameworks for the derivation of occupational or ambient air concentration limits or reference concentrations. HECs that are expressed in terms of aerosol particle sizes experienced by humans but are derived from animal studies allow proper comparison of exposure levels and associated health effects in animals and humans. This can inform differences in responsiveness between animals and humans, based on the same deposited or retained doses and can also allow the use of both data sources in an integrated weight of evidence approach for hazard and risk assessment purposes. Whenever possible, default values should be replaced by substance-specific and target population-specific parameters. Assumptions and sources of uncertainty need to be clearly reported.
Minerva anestesiologica, 1991
![Research paper thumbnail of [Proposal for the methodologic approach to HbCO poisoning]](https://attachments.academia-assets.com/87679551/thumbnails/1.jpg)
](Minerva anestesiologica, 1991
Рассматриваются абелевы группы, имеющие регулярное кольцо эндоморфизмов (регулярный центр кольца ... more Рассматриваются абелевы группы, имеющие регулярное кольцо эндоморфизмов (регулярный центр кольца эндоморфизмов). Ключевые слова: абелева группа, регулярное кольцо эндоморфизмов, регулярный центр кольца эндоморфизмов. and sufficient conditions for the existence of a regular endomorphism ring (of a regular center of the endomorphism ring) of a reduced Abelian group are found.
SAE Technical Paper Series, 1995
In this paper, it is shown how extremely good low temperature activity for CO and hydrocarbons (a... more In this paper, it is shown how extremely good low temperature activity for CO and hydrocarbons (and VOF), negligible storage of sulfates, and very good thermal durability were obtained simultaneously with an appreciable reduction of NOx. Through the systematic analysis of basic catalytic ...
Regulatory Toxicology and Pharmacology, 2001
Journal of the American Chemical Society, 1987
Utilization of aminomethyl-, N-methylaminomethyl-, N,N-dimethylaminomethyl-, and N-acetylaminomet... more Utilization of aminomethyl-, N-methylaminomethyl-, N,N-dimethylaminomethyl-, and N-acetylaminomethylphosphonate by Escherichia coli as a sole source of phosphorus during growth resulted in the extracellular generation of N-methylacetamide, N,N-dimethylacetamide, trimethylamine, and N-methylacetamide, respectively. Product identification relied on synthesis of I3C-enriched aminomethylphosphonates followed by 'H NMR analysis of products isolated from the biodegradation of the labeled and unlabeled phosphorus sources. To circumvent the requirement of an intact cell for carbon to phosphorus bond degradation, transposon mutagenesis was exploited as a complement to the chemical analysis. E. coli K-12 were infected with X::Tn5. Colonies resistant to kanamycin were selected and then screened for loss of the ability to use ethylphosphonate as a sole source of phosphorus. The mutant identified, E. coli SL724, was also unable to degrade aminomethylphosphonates. This combination of chemical and mutagenic analysis points toward a shared mechanism between alkyl-and aminomethylphosphonate biodegradation. Widespread use of agricultural chemicals such as Nphosphonomethylglycine (1) draws attention to the need for understanding degradation and detoxification of these chemicals in the environment.
SEPM Journal of Sedimentary Research, 1974
... 10.--Cross section of plastic impregnated Holoceme oncolite from Joulters Cay area, Great Bah... more ... 10.--Cross section of plastic impregnated Holoceme oncolite from Joulters Cay area, Great Bahama Bank. ... Algal stromatolites de-scribed in this report would be expected to occur in association with burrowed sediments and local cross-bedding; whereas algal structures ...
Environmental Science & Technology, 1993
Thiourea and its lower homologues are uniquely useful in an acidic process for dissolving utility... more Thiourea and its lower homologues are uniquely useful in an acidic process for dissolving utility power plant boiler scales containing iron oxides and copper. The oxidative destruction of thiourea in boiler chemical cleaning waste (BCCW) solutions can be easily accomplished by neutralizing the acid to an excess of the base, adding powdered charcoal and sparging with air or 0 2. The major reaction path leads to dicyandiamide and sodium thiosulfate. A minor reaction path produces urea and sulfur. Using 0.5-2% powdered charcoal and an aeration rate of 7.5 mL/min-l 100 mL-', thiourea is reduced from 2% (0.263 mol/L) to detection limits within 24 h or less.
Biochemistry, 1984
Dehydroquinate synthase has been purified 9000-fold from Escherichia coli K-12 (strain MM294). Th... more Dehydroquinate synthase has been purified 9000-fold from Escherichia coli K-12 (strain MM294). The synthase is encoded by the aroB gene, which is carried by plasmid pLC29-47 from the Carbon-Clarke library. Construction of an appropriate host bearing pLC29-47 results in a strain that produces 20 times more enzyme than strain MM294. Subcloning of the aroB gene behind a tac promoter results in E. coli transformants that produce 1000 times more enzyme than MM294: the synthase constitutes 5% of the soluble protein of the cell. A laborious isolation from 50 g
The American Journal of Forensic Medicine and Pathology, 1988
L'A. reconstruit les premieres etapes de la carriere ecclesiastique de Saint Gregoire Barbari... more L'A. reconstruit les premieres etapes de la carriere ecclesiastique de Saint Gregoire Barbarigo a partir de sa correspondance avec la famille. Il a recu le soutien de l'amitie du Pape Fabius Chigi qui l'a appele a lui a Rome le 15 avril 1656 et qui a fait de lui l'eveque de Bergame en juillet 1657. Des le temps de ses premiers contacts avec le milieu ecclesiastique de la Curie Romaine, Gregoire ne s'est pas comporte comme les ecclesiastiques habituels. Il n'est venu a Rome que pour servir le Pape et accomplir la volonte de celui-ci. A la base de ses convictions, il y avait une tension ethique et spirituelle qui se manifeste dans maints passages de ses lettres
My mother's hand, a cursive trained by nuns, wrote, "Found downstairs you can discard,&q... more My mother's hand, a cursive trained by nuns, wrote, "Found downstairs you can discard," across the packet of her father's death: obit, vital certificate, little filler from the Mirror's back pages flint phrases yanked from a trough of stiff-brimmed prose and the doleful tones of medical examiners. No word of wife or child, just that he fell six flights from his perch on a scaffold. Rain brings out a roofer's best and worst and my mother once drew for us the pitch of the sugar refinery's tall boiler house, where a shining statue of Jack Frost pranced the sky in a pointy hat, tossing handfuls of pure granules from a pouch. Should I describe my grandmother's life, left with eight kids? Instead I'll end with the snow-white sprite who did not become "a victim of the weather," as the tabloid's squib portrayed this immigrant leaving his beginnings near Rome only to land in a railroad car of pig iron in Edgewater, N.J., a town consigned to...
Gift of Dr. Mary Jane Esplen.Piano vocal [instrumentation]G major [key]Allegretto [tempo]Popular ... more Gift of Dr. Mary Jane Esplen.Piano vocal [instrumentation]G major [key]Allegretto [tempo]Popular song ; ragtime [form/genre]Men, dog [illustration]Publisher's advertisement on back cover [note
Journal of Immunotoxicology, 2021
Nickel (Ni) in ambient air may vary regionally with contributions from both natural processes and... more Nickel (Ni) in ambient air may vary regionally with contributions from both natural processes and anthropogenic activities. Exposure to Ni compounds in ambient air above a certain level is associated with acute adverse effects, such as upper respiratory tract irritation, pneumonitis, and chronic adverse effects, such as respiratory cancer. Inhalation reference exposure standards are enacted in different jurisdictions to minimize exposures to ambient Ni above levels that can elicit adverse effects. This paper reports a guideline-/GLP-compliant study designed for setting inhalation exposure standards to protect from immunological effects associated with acute exposure to Ni. Female CD-1 mice were exposed via whole-body inhalation to aerosolized nickel chloride hexahydrate for 24-hr at nominal (vs. mean analyzed) concentrations of 20 (16), 50 (44) and 100 (81) mg Ni/m 3. Host T-cell antibody immunological responses to intravenously-injected sheep red blood cells were then measured ex vivo in an Antibody-Forming Cell (AFC) assay. Exposure to the Ni substance significantly decreased spleen cell levels by 33%, but this was within biological variability for outbred mice. No concurrent decreases in spleen, thymus, or body weights were noted. No immunosuppression was observed with the Ni substance in the context of Total Spleen Activity [IgM AFC/spleen (Â 10 3)] and Specific Activity [IgM AFC/spleen cells (Â 10 6)]. Significant concentration-independent increases in Total Spleen Activity and Specific Activity seen with the nickel chloride hexahydrate were normal and within biological variability for outbred mice. In contrast, cyclophosphamide (positive control) significantly decreased spleen cell numbers, spleen and thymus weights, and abolished Specific Activity and Total Spleen Activity. Based on results here, an NOAEC of 81 mg Ni/m 3 for immunosuppressive effects from inhaled nickel chloride hexahydrate was identified. It is hoped this value can be used to derive a reference standard for human exposure to ambient Ni.
Nature, 1998
Dehydroquinate synthase (DHQS) has long been regarded as a catalytic marvel because of its abilit... more Dehydroquinate synthase (DHQS) has long been regarded as a catalytic marvel because of its ability to perform several consecutive chemical reactions in one active site. There has been considerable debate as to whether DHQS is actively involved in all these steps, or whether several steps occur spontaneously, making DHQS a spectator in its own mechanism. DHQS performs the second step in the shikimate pathway, which is required for the synthesis of aromatic compounds in bacteria, microbial eukaryotes and plants. This enzyme is a potential target for new antifungal and antibacterial drugs as the shikimate pathway is absent from mammals and DHQS is required for pathogen virulence. Here we report the crystal structure of DHQS, which has several unexpected features, including a previously unobserved mode for NAD+-binding and an active-site organization that is surprisingly similar to that of alcohol dehydrogenase, in a new protein fold. The structure reveals interactions between the active site and a substrate-analogue inhibitor, which indicate how DHQS can perform multistep catalysis without the formation of unwanted by-products.
Journal of Aerosol Science, 2016
Dosimetric models are essential tools to refine inhalation risk assessments based on local respir... more Dosimetric models are essential tools to refine inhalation risk assessments based on local respiratory effects. Dosimetric adjustments to account for differences in aerosol particle size and respiratory tract deposition and/or clearance among rodents, workers, and the general public can be applied to experimentally-and epidemiologically-determined points of departure (PODs) to calculate size-selected (e.g., PM 10 , inhalable aerosol fraction, respirable aerosol fraction) equivalent concentrations (e.g., HEC or Human Equivalent Concentration; REC or Rodent Equivalent Concentration). A modified POD (e.g., HEC) can then feed into existing frameworks for the derivation of occupational or ambient air concentration limits or reference concentrations. HECs that are expressed in terms of aerosol particle sizes experienced by humans but are derived from animal studies allow proper comparison of exposure levels and associated health effects in animals and humans. This can inform differences in responsiveness between animals and humans, based on the same deposited or retained doses and can also allow the use of both data sources in an integrated weight of evidence approach for hazard and risk assessment purposes. Whenever possible, default values should be replaced by substance-specific and target population-specific parameters. Assumptions and sources of uncertainty need to be clearly reported.
Minerva anestesiologica, 1991
Minerva anestesiologica, 1991
Рассматриваются абелевы группы, имеющие регулярное кольцо эндоморфизмов (регулярный центр кольца ... more Рассматриваются абелевы группы, имеющие регулярное кольцо эндоморфизмов (регулярный центр кольца эндоморфизмов). Ключевые слова: абелева группа, регулярное кольцо эндоморфизмов, регулярный центр кольца эндоморфизмов. and sufficient conditions for the existence of a regular endomorphism ring (of a regular center of the endomorphism ring) of a reduced Abelian group are found.
SAE Technical Paper Series, 1995
In this paper, it is shown how extremely good low temperature activity for CO and hydrocarbons (a... more In this paper, it is shown how extremely good low temperature activity for CO and hydrocarbons (and VOF), negligible storage of sulfates, and very good thermal durability were obtained simultaneously with an appreciable reduction of NOx. Through the systematic analysis of basic catalytic ...
Regulatory Toxicology and Pharmacology, 2001
Journal of the American Chemical Society, 1987
Utilization of aminomethyl-, N-methylaminomethyl-, N,N-dimethylaminomethyl-, and N-acetylaminomet... more Utilization of aminomethyl-, N-methylaminomethyl-, N,N-dimethylaminomethyl-, and N-acetylaminomethylphosphonate by Escherichia coli as a sole source of phosphorus during growth resulted in the extracellular generation of N-methylacetamide, N,N-dimethylacetamide, trimethylamine, and N-methylacetamide, respectively. Product identification relied on synthesis of I3C-enriched aminomethylphosphonates followed by 'H NMR analysis of products isolated from the biodegradation of the labeled and unlabeled phosphorus sources. To circumvent the requirement of an intact cell for carbon to phosphorus bond degradation, transposon mutagenesis was exploited as a complement to the chemical analysis. E. coli K-12 were infected with X::Tn5. Colonies resistant to kanamycin were selected and then screened for loss of the ability to use ethylphosphonate as a sole source of phosphorus. The mutant identified, E. coli SL724, was also unable to degrade aminomethylphosphonates. This combination of chemical and mutagenic analysis points toward a shared mechanism between alkyl-and aminomethylphosphonate biodegradation. Widespread use of agricultural chemicals such as Nphosphonomethylglycine (1) draws attention to the need for understanding degradation and detoxification of these chemicals in the environment.
SEPM Journal of Sedimentary Research, 1974
... 10.--Cross section of plastic impregnated Holoceme oncolite from Joulters Cay area, Great Bah... more ... 10.--Cross section of plastic impregnated Holoceme oncolite from Joulters Cay area, Great Bahama Bank. ... Algal stromatolites de-scribed in this report would be expected to occur in association with burrowed sediments and local cross-bedding; whereas algal structures ...
Environmental Science & Technology, 1993
Thiourea and its lower homologues are uniquely useful in an acidic process for dissolving utility... more Thiourea and its lower homologues are uniquely useful in an acidic process for dissolving utility power plant boiler scales containing iron oxides and copper. The oxidative destruction of thiourea in boiler chemical cleaning waste (BCCW) solutions can be easily accomplished by neutralizing the acid to an excess of the base, adding powdered charcoal and sparging with air or 0 2. The major reaction path leads to dicyandiamide and sodium thiosulfate. A minor reaction path produces urea and sulfur. Using 0.5-2% powdered charcoal and an aeration rate of 7.5 mL/min-l 100 mL-', thiourea is reduced from 2% (0.263 mol/L) to detection limits within 24 h or less.
Biochemistry, 1984
Dehydroquinate synthase has been purified 9000-fold from Escherichia coli K-12 (strain MM294). Th... more Dehydroquinate synthase has been purified 9000-fold from Escherichia coli K-12 (strain MM294). The synthase is encoded by the aroB gene, which is carried by plasmid pLC29-47 from the Carbon-Clarke library. Construction of an appropriate host bearing pLC29-47 results in a strain that produces 20 times more enzyme than strain MM294. Subcloning of the aroB gene behind a tac promoter results in E. coli transformants that produce 1000 times more enzyme than MM294: the synthase constitutes 5% of the soluble protein of the cell. A laborious isolation from 50 g
The American Journal of Forensic Medicine and Pathology, 1988
L'A. reconstruit les premieres etapes de la carriere ecclesiastique de Saint Gregoire Barbari... more L'A. reconstruit les premieres etapes de la carriere ecclesiastique de Saint Gregoire Barbarigo a partir de sa correspondance avec la famille. Il a recu le soutien de l'amitie du Pape Fabius Chigi qui l'a appele a lui a Rome le 15 avril 1656 et qui a fait de lui l'eveque de Bergame en juillet 1657. Des le temps de ses premiers contacts avec le milieu ecclesiastique de la Curie Romaine, Gregoire ne s'est pas comporte comme les ecclesiastiques habituels. Il n'est venu a Rome que pour servir le Pape et accomplir la volonte de celui-ci. A la base de ses convictions, il y avait une tension ethique et spirituelle qui se manifeste dans maints passages de ses lettres