Jacob Antony - Academia.edu (original) (raw)

Papers by Jacob Antony

Research paper thumbnail of Toxicological effects of silver nanoparticles

Environmental Toxicology and Pharmacology, 2015

The effects of silver nanoparticles (Ag-NPs) on the growth, metamorphosis, innate immune paramete... more The effects of silver nanoparticles (Ag-NPs) on the growth, metamorphosis, innate immune parameters and enzyme profiles of tadpole, Polypedates maculatus were studied. Chronic exposure to Ag-NPs for 60 days at 1 mg l-1 and 5 mg l-1 concentrations caused 10% and 50% mortalities respectively alongwith slow growth and delayed metamorphosis as compared to 0.1 mg l-1 treatment and control (0 mg l-1). The non-specific immune parameters and certain enzyme activities like alkaline phosphatase (ALP), superoxide dismutase (SOD) and lactate dehydrogenase (LDH) varied significantly in a dose dependent manner. The accumulation of silver in the tadpole tissues of treated groups ranged from 0.0966±0.0025 µg g-1 to 0.4718±0.0126 µg g-1 as compared to tissues in control group (0.0758±0.0019 µg g-1). Hence Ag-NPs had significant detrimental effects on growth and other associated physiological parameters of tadpole, Polypedates maculatus when administered at higher concentrations (>1 mg l-1) for longer durations. Thus the toxicity studies of Ag-NPs have to be conducted on wider ranges of target and non-target species to assess their environmental impacts. Furthermore, these results suggest that silver nanoparticles induce a dose-dependent toxicity in physiological parameters of tadpoles, which hinders normal development.This study was aimed to enhance our insight on the health and environmental impact of silver nanoparticles. This study pointed out the adverse effects of Ag-NPs in tadpole species and all applications involving silver nanoparticles should be given special attention and promoted only after detailed studies.

Research paper thumbnail of Cytotoxic effect of Green synthesized silver nanoparticles using Melia azedarach against in vitro HeLa cell lines and lymphoma mice model

Process Biochemistry, 2012

Research paper thumbnail of In vivo antitumor activity of biosynthesized silver nanoparticles using Ficus religiosa as a nanofactory in DAL induced mice model

Colloids and Surfaces B: Biointerfaces, 2013

Research paper thumbnail of Antimicrobial activity of Leucas aspera engineered silver nanoparticles against Aeromonas hydrophila in infected Catla catla

Colloids and Surfaces B: Biointerfaces, 2013

Research paper thumbnail of Comparative evaluation of antibacterial activity of silver nanoparticles synthesized using Rhizophora apiculata and glucose

Colloids and Surfaces B: Biointerfaces, 2011

h i g h l i g h t s • Synthesis of silver nanoparticles using Rhizophora apiculata and glucose. •... more h i g h l i g h t s • Synthesis of silver nanoparticles using Rhizophora apiculata and glucose. • Characterization of nanoparticles by UV, FTIR and TEM. • Comparison of antimicrobial efficacy of the silver nanoparticles of biological and chemical origin.

Research paper thumbnail of Mangrove Streptomyces sp. BDUKAS10 as nanofactory for fabrication of bactericidal silver nanoparticles

Colloids and Surfaces B: Biointerfaces, 2012

Biosynthesis has led to the development of various biomimetic approaches for the fabrication of n... more Biosynthesis has led to the development of various biomimetic approaches for the fabrication of nanoscale materials. The present study reveals a unique procedure for the biosynthesis of bactericidal silver nanoparticles (AgNPs) using a novel Streptomyces sp. BDUKAS10, an isolate of mangrove sediment. Aqueous silver nitrate (AgNO(3)) solution was treated with cell free supernatant (CFS) of the isolate to synthesize bactericidal silver nanoparticles. Initial characterization was performed by visual observation for color change to intense brown color. UV-visible spectrophotometry (UV-vis) for measuring surface plasmon resonance indicated a maximum absorption peak at 441 nm. Fourier Transform Infrared Spectroscopy (FTIR) analysis provides evidence for proteins as possible reducing, and capping agents. Energy dispersive X-ray (EDAX) spectroscopy analysis showed elemental silver as major signal. Transmission Electron Microscopy (TEM) study indicated spherical silver nanoparticles in the size range of 21-48 nm. Compared to the CFS, the biosynthesized AgNPs exemplified superior bactericidal efficacy towards the tested bacterial strains. Results from this study suggested that Streptomyces sp. BDUKAS10 can be advantageous for the synthesis of AgNPs by extracellular method in the view of sustainable and ecofriendly approach.

Research paper thumbnail of Hepatocurative activity of biosynthesized silver nanoparticles fabricated using Andrographis paniculata

Colloids and Surfaces B: Biointerfaces, 2013

The current investigation was aimed to determine the hepatocurative role of silver nanoparticles ... more The current investigation was aimed to determine the hepatocurative role of silver nanoparticles (AgNPs) synthesized rapidly using Andrographis paniculata. The nanoparticles fabricated at varying temperatures were characterized by UV-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), fourier transform infra-red spectroscopy (FTIR), energy dispersive X-ray (EDX) and inductively coupled plasma optical emission spectroscopy (ICP-OES) alongside zeta potential measurement. UV-vis spectroscopic readings indicated a prominent peak at 423 nm. TEM analysis indicated that the biosynthesized nanospheres were in the size range of 13-27 nm. EDX spectrum indicated strong signal for AgNPs with 90.1% purity. The total concentration of AgNps was 216.7 mg/L after synthesis as by ICP-OES. Zeta potential was -34.3 mV indicating stable AgNPs. In vitro radical scavenging assay proved strong antioxidant effect of the AgNPs compared to 5% aqueous leaf extract. CCl(4) was used to induce hepatic injury in mice model. The biosynthesized AgNPs at three different doses (25, 50, 100mg/kg BW of the animal) were used for treatment. Silymarin was used as a standard. Low dose (25mg/kg BW) was effective in revival of all biological parameters to near normal in all intoxicated groups indicating the curing effects on CCl(4) induced liver injury.

Research paper thumbnail of Toxicological effects of silver nanoparticles

Environmental Toxicology and Pharmacology, 2015

The effects of silver nanoparticles (Ag-NPs) on the growth, metamorphosis, innate immune paramete... more The effects of silver nanoparticles (Ag-NPs) on the growth, metamorphosis, innate immune parameters and enzyme profiles of tadpole, Polypedates maculatus were studied. Chronic exposure to Ag-NPs for 60 days at 1 mg l-1 and 5 mg l-1 concentrations caused 10% and 50% mortalities respectively alongwith slow growth and delayed metamorphosis as compared to 0.1 mg l-1 treatment and control (0 mg l-1). The non-specific immune parameters and certain enzyme activities like alkaline phosphatase (ALP), superoxide dismutase (SOD) and lactate dehydrogenase (LDH) varied significantly in a dose dependent manner. The accumulation of silver in the tadpole tissues of treated groups ranged from 0.0966±0.0025 µg g-1 to 0.4718±0.0126 µg g-1 as compared to tissues in control group (0.0758±0.0019 µg g-1). Hence Ag-NPs had significant detrimental effects on growth and other associated physiological parameters of tadpole, Polypedates maculatus when administered at higher concentrations (>1 mg l-1) for longer durations. Thus the toxicity studies of Ag-NPs have to be conducted on wider ranges of target and non-target species to assess their environmental impacts. Furthermore, these results suggest that silver nanoparticles induce a dose-dependent toxicity in physiological parameters of tadpoles, which hinders normal development.This study was aimed to enhance our insight on the health and environmental impact of silver nanoparticles. This study pointed out the adverse effects of Ag-NPs in tadpole species and all applications involving silver nanoparticles should be given special attention and promoted only after detailed studies.

Research paper thumbnail of Cytotoxic effect of Green synthesized silver nanoparticles using Melia azedarach against in vitro HeLa cell lines and lymphoma mice model

Process Biochemistry, 2012

Research paper thumbnail of In vivo antitumor activity of biosynthesized silver nanoparticles using Ficus religiosa as a nanofactory in DAL induced mice model

Colloids and Surfaces B: Biointerfaces, 2013

Research paper thumbnail of Antimicrobial activity of Leucas aspera engineered silver nanoparticles against Aeromonas hydrophila in infected Catla catla

Colloids and Surfaces B: Biointerfaces, 2013

Research paper thumbnail of Comparative evaluation of antibacterial activity of silver nanoparticles synthesized using Rhizophora apiculata and glucose

Colloids and Surfaces B: Biointerfaces, 2011

h i g h l i g h t s • Synthesis of silver nanoparticles using Rhizophora apiculata and glucose. •... more h i g h l i g h t s • Synthesis of silver nanoparticles using Rhizophora apiculata and glucose. • Characterization of nanoparticles by UV, FTIR and TEM. • Comparison of antimicrobial efficacy of the silver nanoparticles of biological and chemical origin.

Research paper thumbnail of Mangrove Streptomyces sp. BDUKAS10 as nanofactory for fabrication of bactericidal silver nanoparticles

Colloids and Surfaces B: Biointerfaces, 2012

Biosynthesis has led to the development of various biomimetic approaches for the fabrication of n... more Biosynthesis has led to the development of various biomimetic approaches for the fabrication of nanoscale materials. The present study reveals a unique procedure for the biosynthesis of bactericidal silver nanoparticles (AgNPs) using a novel Streptomyces sp. BDUKAS10, an isolate of mangrove sediment. Aqueous silver nitrate (AgNO(3)) solution was treated with cell free supernatant (CFS) of the isolate to synthesize bactericidal silver nanoparticles. Initial characterization was performed by visual observation for color change to intense brown color. UV-visible spectrophotometry (UV-vis) for measuring surface plasmon resonance indicated a maximum absorption peak at 441 nm. Fourier Transform Infrared Spectroscopy (FTIR) analysis provides evidence for proteins as possible reducing, and capping agents. Energy dispersive X-ray (EDAX) spectroscopy analysis showed elemental silver as major signal. Transmission Electron Microscopy (TEM) study indicated spherical silver nanoparticles in the size range of 21-48 nm. Compared to the CFS, the biosynthesized AgNPs exemplified superior bactericidal efficacy towards the tested bacterial strains. Results from this study suggested that Streptomyces sp. BDUKAS10 can be advantageous for the synthesis of AgNPs by extracellular method in the view of sustainable and ecofriendly approach.

Research paper thumbnail of Hepatocurative activity of biosynthesized silver nanoparticles fabricated using Andrographis paniculata

Colloids and Surfaces B: Biointerfaces, 2013

The current investigation was aimed to determine the hepatocurative role of silver nanoparticles ... more The current investigation was aimed to determine the hepatocurative role of silver nanoparticles (AgNPs) synthesized rapidly using Andrographis paniculata. The nanoparticles fabricated at varying temperatures were characterized by UV-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), fourier transform infra-red spectroscopy (FTIR), energy dispersive X-ray (EDX) and inductively coupled plasma optical emission spectroscopy (ICP-OES) alongside zeta potential measurement. UV-vis spectroscopic readings indicated a prominent peak at 423 nm. TEM analysis indicated that the biosynthesized nanospheres were in the size range of 13-27 nm. EDX spectrum indicated strong signal for AgNPs with 90.1% purity. The total concentration of AgNps was 216.7 mg/L after synthesis as by ICP-OES. Zeta potential was -34.3 mV indicating stable AgNPs. In vitro radical scavenging assay proved strong antioxidant effect of the AgNPs compared to 5% aqueous leaf extract. CCl(4) was used to induce hepatic injury in mice model. The biosynthesized AgNPs at three different doses (25, 50, 100mg/kg BW of the animal) were used for treatment. Silymarin was used as a standard. Low dose (25mg/kg BW) was effective in revival of all biological parameters to near normal in all intoxicated groups indicating the curing effects on CCl(4) induced liver injury.