Laurent Jacob - Academia.edu (original) (raw)
Papers by Laurent Jacob
Annales Françaises d Anesthésie et de Réanimation
Annales Françaises d Anesthésie et de Réanimation
Anaesthesia Critical Care & Pain Medicine, 2015
Journal of Thoracic and Cardiovascular Surgery
Between 1968 and 1988, 679 patients were hospitalized for ingestion of caustic substances, and 87... more Between 1968 and 1988, 679 patients were hospitalized for ingestion of caustic substances, and 87 had severe caustic burns of the entire esophagus, together with panparietal necrosis. Twenty-one of them had tracheobronchial necrosis with perforation. Fifteen have not been operated on; six have had operations, with success in four. We describe an original technique for repairing these tracheobronchial perforations with a pulmonary patch.
European Journal of Anaesthesiology
Accidental intra-arterial injection of a colouring agent (Bonney's blue) in a 28-year-old mal... more Accidental intra-arterial injection of a colouring agent (Bonney's blue) in a 28-year-old male patient induced acute ischaemia of the lower limb, resistant to standard therapy. Its successful treatment was finally obtained by intra-arterial infusion of nicardipine.
Journal of Thoracic and Cardiovascular Surgery
Annales Françaises d Anesthésie et de Réanimation
Journal of Thoracic and Cardiovascular Surgery
Regional ischemia may induce cervical anastomosis leakage or stenosis or graft necrosis after eso... more Regional ischemia may induce cervical anastomosis leakage or stenosis or graft necrosis after esophageal reconstruction by retrosternal interposition of an ileocolic graft. These complications may be related to systemic or local hemodynamic alterations. This study was designed to evaluate the relationship between immediate postoperative arterial blood supply to the graft, arterial patency monitored by angiography, and clinical outcome. Eight patients (mean age 30 +/- 4 years; standard deviation) were studied. Miniaturized Doppler implantable microprobes were sutured to the single artery supplying the graft and connected to an 8 MHz pulsed Doppler flowmeter. Systemic hemodynamic parameters and mesenteric hemodynamic data were collected 3 hours after the end of the surgical procedure. These data were compared with the angiogram of the right superior colic artery supplying the graft, systematically performed on the fifteenth postoperative day, and with the clinical course of follow-up ...
Annales Françaises d Anesthésie et de Réanimation
EMC - Médecine d 'urgence, 2010
ABSTRACT L’insuffisance rénale aiguë (IRA) est une altération récente de la fonction rénale qui e... more ABSTRACT L’insuffisance rénale aiguë (IRA) est une altération récente de la fonction rénale qui engage le pronostic vital du patient à court terme et le pronostic fonctionnel de l’organe à plus long terme. Cette dysfonction d’organe relativement fréquente fait l’objet d’une évolution constante des définitions et des connaissances. L’évolution des populations présentant cette dysfonction d’organe montre une majorité d’IRA survenant en réanimation dans un contexte sévère de syndrome de défaillance multiviscérale. La gravité pronostique aussi bien en termes de mortalité que d’insuffisance rénale chronique séquellaire souligne la nécessité de prévenir la survenue de cette complication, notamment dans les situations où le risque est connu et identifié comme la période périopératoire. Le maintien d’une perfusion rénale appropriée semble rester un élément essentiel de cette stratégie préventive, aucune intervention pharmacologique n’ayant fait sa preuve. La détection précoce des situations à risque (patients, polypathologies, médicaments, type de chirurgie, etc.) permet la restauration rapide de la perfusion rénale et l’utilisation sélective de mesures de protection appropriées. L’idéal serait à l’avenir de privilégier l’utilisation de marqueurs d’agressions rénales et de souffrances tubulaires, beaucoup plus précoces que les marqueurs de dysfonction rénale fondés sur l’altération de la filtration glomérulaire, qui se situe à la fin du processus pathologique.
BMC Bioinformatics, 2015
Detecting and quantifying isoforms from RNA-seq data is an important but challenging task. The pr... more Detecting and quantifying isoforms from RNA-seq data is an important but challenging task. The problem is often ill-posed, particularly at low coverage. One promising direction is to exploit several samples simultaneously. We propose a new method for solving the isoform deconvolution problem jointly across several samples. We formulate a convex optimization problem that allows to share information between samples and that we solve efficiently. We demonstrate the benefits of combining several samples on simulated and real data, and show that our approach outperforms pooling strategies and methods based on integer programming. Our convex formulation to jointly detect and quantify isoforms from RNA-seq data of multiple related samples is a computationally efficient approach to leverage the hypotheses that some isoforms are likely to be present in several samples. The software and source code are available at http://cbio.ensmp.fr/flipflop .
Journal Européen des Urgences, 2008
passage au SAU et à la CCPU est de 22,6 jours. La sex-ratio est de 0,512 et l'âge moyen de 49,9 a... more passage au SAU et à la CCPU est de 22,6 jours. La sex-ratio est de 0,512 et l'âge moyen de 49,9 ans (AE 9,2). Vingt-cinq patients (59,5 %) ont bénéficié d'un CRH du SAU et 100 % d'un courrier du cardiologue adressé au médecin traitant et au SAU.
EMC - Anesthésie-Réanimation, 2004
Journal Européen des Urgences, 2008
EMC - Anesthésie-Réanimation, 2004
ABSTRACT Urinary tract surgery may be carried out in patients of any age. However, prostate surge... more ABSTRACT Urinary tract surgery may be carried out in patients of any age. However, prostate surgery concerns elderly patients and potentially underlying cardiopulmonary diseases. The infectious risk is an important issue to consider as this surgery is a clean-contaminated process that requires therefore urine sterility and antibiotic prophylaxis before any act. Laparoscopy plays an increasing role in carcinologic surgery by lowering blood loss, post operative pain, and hospital stay. Indications still remain for open surgery. Kidney surgery by section of the loins exposes patients to cardiorespiratory complications mostly due to perioperative position. Prostate and bladder surgeries require a good knowledge of urinary drainage and derivation techniques. Endoscopic surgery still plays an important role in the cure of benign prostate hypertrophy, bladder tumours and lithiasic pathology. Nevertheless, irrigation liquid re-absorption remains a risk. It can be prevented, as its clinical manifestation, the TURP syndrome, by the controlled use of glycocolle.
Annales françaises d'anesthèsie et de rèanimation, 2004
The immune response against a bacterial aggression involves the monocytes-macrophages and polymor... more The immune response against a bacterial aggression involves the monocytes-macrophages and polymorphonuclear neutrophils (PMN) in the first line of defense. This natural or innate immunity controls the proliferation of micro-organisms while waiting for the development of aspecific immunity related to lymphocytes. Establishing a link between innate and specific immunity, interleukin-12 (IL-12) is an essential cytokine of the inflammatory response. In a first in vitro study, we showed that IL-12 potentiates the effect of LPS on the production of IL-8 by stimulated PMN, the main chemotactic and activating cytokine of neutrophils. IL-12 would thus support the local recruitment of PMN via an autocrine loop of amplification. In a second in vivo study in septic patients, we noted a defect in the pulmonary and systemic production of IL-12, suggesting a dysregulation of innate immunity during the course of sepsis.
Journal of leukocyte biology, 2001
Interleukin (IL) 12 is a heterodimeric cytokine mainly produced by phagocytes-important target ce... more Interleukin (IL) 12 is a heterodimeric cytokine mainly produced by phagocytes-important target cells for IL-12 in particular with a chemotactic effect-and antigen-presenting cells in response to various microorganisms. Because IL-8 is a strong chemokine for polymorphonuclear neutrophils (PMNs), we investigated the effect of IL-12 on PMN IL-8 production. IL-12 alone had no significant effect, but with lipopolysaccharide (LPS) it was additive at both protein and mRNA levels. Actinomycin D at the beginning of culture inhibited IL-8 mRNA induction, whereas late addition affected IL-8 transcript stability, suggesting gene transcription involvement. Results with parthenolide and tyrphostin AG490 suggest that nuclear factor-kappaB and signal transducer and activator of transcription 4 play a role. The IL-12 additive effect was restricted to IL-8 release, with no action on cell-associated IL-8. IL-12 additive effects occurred after 18 h of culture, with no marked up-regulation of IL-12 rece...
The Journal of thoracic and cardiovascular surgery, 1994
Esophageal replacement after esophagogastric injury caused by ingestion of lye may require the in... more Esophageal replacement after esophagogastric injury caused by ingestion of lye may require the interposition of a retrosternal ileocolic graft. In this new anatomic situation, the mesenteric circulation supplying the graft is subjected to the intrathoracic pressure surrounding the graft. Thus, mesenteric blood flow supplying the graft may be impaired when intrathoracic pressure is increased during mechanical ventilation. This study was designed to evaluate the effect of increasing intrathoracic pressure by application of a positive end-expiratory pressure on mesenteric blood flow supplying esophageal ileocolic grafts. Eight cases were studied in the immediate postoperative period. Miniaturized implantable Doppler microprobes were sutured to the single artery supplying the graft and connected to an 8 MHz pulsed Doppler flowmeter. Two sets of measurements were successively performed with zero end-expiratory pressure ventilation and after application of a 15 cm water positive end-expir...
Nutrition (Burbank, Los Angeles County, Calif.)
The accuracy of paired quantitative blood cultures (PQtBCs) collected in pediatric Isolator 1.5-m... more The accuracy of paired quantitative blood cultures (PQtBCs) collected in pediatric Isolator 1.5-ml tubes compared to central venous catheter (CVC) segment cultures (hub and tip) to diagnose catheter-related bacteremia (CRB) was evaluated in 58 bacteremic adult patients. The second aim of this study was to state precisely whether the tip or the hub (or both) of the infected device was the source of the bacteremia in case of significant results of PQtBC. Fifty-eight bacteremic patients with suspected CRB entered the study. In 52 patients, the diagnosis was obtained before CVC removal by PQtBC and was confirmed by CVC segment cultures: CRB in 30 patients, non-catheter-related bacteremia in 22 patients. Six patients had CRB not found by PQtBC. 1) PQtBC is 83% sensitive, 100% specific (negative predictive values 78%, positive predictive values 100%). 2) Sixteen bacteremic patients had authentic hub-related bacteremia (positive hub culture associated with negative tip cultures). When CRB ...
Proceedings of the National Academy of Sciences of the United States of America, 1984
A murine monoclonal anti-DNA antibody ( PME77 ) has been found to bind tightly to the plasma memb... more A murine monoclonal anti-DNA antibody ( PME77 ) has been found to bind tightly to the plasma membrane of Raji cells. We show here that this monoclonal anti-DNA antibody reacts in a radioimmunoassay with the cell surface of a variety of mammalian cell types and that the antigenic determinant recognized by the monoclonal anti-DNA antibody at the surface of Raji cells is resistant to DNase. It belongs to polypeptides removed from the cell surface by a mild proteinase K treatment.
Annales Françaises d Anesthésie et de Réanimation
Annales Françaises d Anesthésie et de Réanimation
Anaesthesia Critical Care & Pain Medicine, 2015
Journal of Thoracic and Cardiovascular Surgery
Between 1968 and 1988, 679 patients were hospitalized for ingestion of caustic substances, and 87... more Between 1968 and 1988, 679 patients were hospitalized for ingestion of caustic substances, and 87 had severe caustic burns of the entire esophagus, together with panparietal necrosis. Twenty-one of them had tracheobronchial necrosis with perforation. Fifteen have not been operated on; six have had operations, with success in four. We describe an original technique for repairing these tracheobronchial perforations with a pulmonary patch.
European Journal of Anaesthesiology
Accidental intra-arterial injection of a colouring agent (Bonney's blue) in a 28-year-old mal... more Accidental intra-arterial injection of a colouring agent (Bonney's blue) in a 28-year-old male patient induced acute ischaemia of the lower limb, resistant to standard therapy. Its successful treatment was finally obtained by intra-arterial infusion of nicardipine.
Journal of Thoracic and Cardiovascular Surgery
Annales Françaises d Anesthésie et de Réanimation
Journal of Thoracic and Cardiovascular Surgery
Regional ischemia may induce cervical anastomosis leakage or stenosis or graft necrosis after eso... more Regional ischemia may induce cervical anastomosis leakage or stenosis or graft necrosis after esophageal reconstruction by retrosternal interposition of an ileocolic graft. These complications may be related to systemic or local hemodynamic alterations. This study was designed to evaluate the relationship between immediate postoperative arterial blood supply to the graft, arterial patency monitored by angiography, and clinical outcome. Eight patients (mean age 30 +/- 4 years; standard deviation) were studied. Miniaturized Doppler implantable microprobes were sutured to the single artery supplying the graft and connected to an 8 MHz pulsed Doppler flowmeter. Systemic hemodynamic parameters and mesenteric hemodynamic data were collected 3 hours after the end of the surgical procedure. These data were compared with the angiogram of the right superior colic artery supplying the graft, systematically performed on the fifteenth postoperative day, and with the clinical course of follow-up ...
Annales Françaises d Anesthésie et de Réanimation
EMC - Médecine d 'urgence, 2010
ABSTRACT L’insuffisance rénale aiguë (IRA) est une altération récente de la fonction rénale qui e... more ABSTRACT L’insuffisance rénale aiguë (IRA) est une altération récente de la fonction rénale qui engage le pronostic vital du patient à court terme et le pronostic fonctionnel de l’organe à plus long terme. Cette dysfonction d’organe relativement fréquente fait l’objet d’une évolution constante des définitions et des connaissances. L’évolution des populations présentant cette dysfonction d’organe montre une majorité d’IRA survenant en réanimation dans un contexte sévère de syndrome de défaillance multiviscérale. La gravité pronostique aussi bien en termes de mortalité que d’insuffisance rénale chronique séquellaire souligne la nécessité de prévenir la survenue de cette complication, notamment dans les situations où le risque est connu et identifié comme la période périopératoire. Le maintien d’une perfusion rénale appropriée semble rester un élément essentiel de cette stratégie préventive, aucune intervention pharmacologique n’ayant fait sa preuve. La détection précoce des situations à risque (patients, polypathologies, médicaments, type de chirurgie, etc.) permet la restauration rapide de la perfusion rénale et l’utilisation sélective de mesures de protection appropriées. L’idéal serait à l’avenir de privilégier l’utilisation de marqueurs d’agressions rénales et de souffrances tubulaires, beaucoup plus précoces que les marqueurs de dysfonction rénale fondés sur l’altération de la filtration glomérulaire, qui se situe à la fin du processus pathologique.
BMC Bioinformatics, 2015
Detecting and quantifying isoforms from RNA-seq data is an important but challenging task. The pr... more Detecting and quantifying isoforms from RNA-seq data is an important but challenging task. The problem is often ill-posed, particularly at low coverage. One promising direction is to exploit several samples simultaneously. We propose a new method for solving the isoform deconvolution problem jointly across several samples. We formulate a convex optimization problem that allows to share information between samples and that we solve efficiently. We demonstrate the benefits of combining several samples on simulated and real data, and show that our approach outperforms pooling strategies and methods based on integer programming. Our convex formulation to jointly detect and quantify isoforms from RNA-seq data of multiple related samples is a computationally efficient approach to leverage the hypotheses that some isoforms are likely to be present in several samples. The software and source code are available at http://cbio.ensmp.fr/flipflop .
Journal Européen des Urgences, 2008
passage au SAU et à la CCPU est de 22,6 jours. La sex-ratio est de 0,512 et l'âge moyen de 49,9 a... more passage au SAU et à la CCPU est de 22,6 jours. La sex-ratio est de 0,512 et l'âge moyen de 49,9 ans (AE 9,2). Vingt-cinq patients (59,5 %) ont bénéficié d'un CRH du SAU et 100 % d'un courrier du cardiologue adressé au médecin traitant et au SAU.
EMC - Anesthésie-Réanimation, 2004
Journal Européen des Urgences, 2008
EMC - Anesthésie-Réanimation, 2004
ABSTRACT Urinary tract surgery may be carried out in patients of any age. However, prostate surge... more ABSTRACT Urinary tract surgery may be carried out in patients of any age. However, prostate surgery concerns elderly patients and potentially underlying cardiopulmonary diseases. The infectious risk is an important issue to consider as this surgery is a clean-contaminated process that requires therefore urine sterility and antibiotic prophylaxis before any act. Laparoscopy plays an increasing role in carcinologic surgery by lowering blood loss, post operative pain, and hospital stay. Indications still remain for open surgery. Kidney surgery by section of the loins exposes patients to cardiorespiratory complications mostly due to perioperative position. Prostate and bladder surgeries require a good knowledge of urinary drainage and derivation techniques. Endoscopic surgery still plays an important role in the cure of benign prostate hypertrophy, bladder tumours and lithiasic pathology. Nevertheless, irrigation liquid re-absorption remains a risk. It can be prevented, as its clinical manifestation, the TURP syndrome, by the controlled use of glycocolle.
Annales françaises d'anesthèsie et de rèanimation, 2004
The immune response against a bacterial aggression involves the monocytes-macrophages and polymor... more The immune response against a bacterial aggression involves the monocytes-macrophages and polymorphonuclear neutrophils (PMN) in the first line of defense. This natural or innate immunity controls the proliferation of micro-organisms while waiting for the development of aspecific immunity related to lymphocytes. Establishing a link between innate and specific immunity, interleukin-12 (IL-12) is an essential cytokine of the inflammatory response. In a first in vitro study, we showed that IL-12 potentiates the effect of LPS on the production of IL-8 by stimulated PMN, the main chemotactic and activating cytokine of neutrophils. IL-12 would thus support the local recruitment of PMN via an autocrine loop of amplification. In a second in vivo study in septic patients, we noted a defect in the pulmonary and systemic production of IL-12, suggesting a dysregulation of innate immunity during the course of sepsis.
Journal of leukocyte biology, 2001
Interleukin (IL) 12 is a heterodimeric cytokine mainly produced by phagocytes-important target ce... more Interleukin (IL) 12 is a heterodimeric cytokine mainly produced by phagocytes-important target cells for IL-12 in particular with a chemotactic effect-and antigen-presenting cells in response to various microorganisms. Because IL-8 is a strong chemokine for polymorphonuclear neutrophils (PMNs), we investigated the effect of IL-12 on PMN IL-8 production. IL-12 alone had no significant effect, but with lipopolysaccharide (LPS) it was additive at both protein and mRNA levels. Actinomycin D at the beginning of culture inhibited IL-8 mRNA induction, whereas late addition affected IL-8 transcript stability, suggesting gene transcription involvement. Results with parthenolide and tyrphostin AG490 suggest that nuclear factor-kappaB and signal transducer and activator of transcription 4 play a role. The IL-12 additive effect was restricted to IL-8 release, with no action on cell-associated IL-8. IL-12 additive effects occurred after 18 h of culture, with no marked up-regulation of IL-12 rece...
The Journal of thoracic and cardiovascular surgery, 1994
Esophageal replacement after esophagogastric injury caused by ingestion of lye may require the in... more Esophageal replacement after esophagogastric injury caused by ingestion of lye may require the interposition of a retrosternal ileocolic graft. In this new anatomic situation, the mesenteric circulation supplying the graft is subjected to the intrathoracic pressure surrounding the graft. Thus, mesenteric blood flow supplying the graft may be impaired when intrathoracic pressure is increased during mechanical ventilation. This study was designed to evaluate the effect of increasing intrathoracic pressure by application of a positive end-expiratory pressure on mesenteric blood flow supplying esophageal ileocolic grafts. Eight cases were studied in the immediate postoperative period. Miniaturized implantable Doppler microprobes were sutured to the single artery supplying the graft and connected to an 8 MHz pulsed Doppler flowmeter. Two sets of measurements were successively performed with zero end-expiratory pressure ventilation and after application of a 15 cm water positive end-expir...
Nutrition (Burbank, Los Angeles County, Calif.)
The accuracy of paired quantitative blood cultures (PQtBCs) collected in pediatric Isolator 1.5-m... more The accuracy of paired quantitative blood cultures (PQtBCs) collected in pediatric Isolator 1.5-ml tubes compared to central venous catheter (CVC) segment cultures (hub and tip) to diagnose catheter-related bacteremia (CRB) was evaluated in 58 bacteremic adult patients. The second aim of this study was to state precisely whether the tip or the hub (or both) of the infected device was the source of the bacteremia in case of significant results of PQtBC. Fifty-eight bacteremic patients with suspected CRB entered the study. In 52 patients, the diagnosis was obtained before CVC removal by PQtBC and was confirmed by CVC segment cultures: CRB in 30 patients, non-catheter-related bacteremia in 22 patients. Six patients had CRB not found by PQtBC. 1) PQtBC is 83% sensitive, 100% specific (negative predictive values 78%, positive predictive values 100%). 2) Sixteen bacteremic patients had authentic hub-related bacteremia (positive hub culture associated with negative tip cultures). When CRB ...
Proceedings of the National Academy of Sciences of the United States of America, 1984
A murine monoclonal anti-DNA antibody ( PME77 ) has been found to bind tightly to the plasma memb... more A murine monoclonal anti-DNA antibody ( PME77 ) has been found to bind tightly to the plasma membrane of Raji cells. We show here that this monoclonal anti-DNA antibody reacts in a radioimmunoassay with the cell surface of a variety of mammalian cell types and that the antigenic determinant recognized by the monoclonal anti-DNA antibody at the surface of Raji cells is resistant to DNase. It belongs to polypeptides removed from the cell surface by a mild proteinase K treatment.