Jader Nones - Academia.edu (original) (raw)

Papers by Jader Nones

Pubvet, Jul 1, 2018

RESUMO. A movimentação animal é um dos principais meios de disseminação de doenças. Muitas destas... more RESUMO. A movimentação animal é um dos principais meios de disseminação de doenças. Muitas destas, pelos riscos e impactos na cadeia produtiva, fazem parte de programas de controle do Serviço de Defesa Sanitária Oficial de cada país. Com o intuito de evitar a dispersão de agentes patogênicos no Brasil, diretrizes para este controle são determinadas pelo Ministério da Agricultura, Pecuária e Abastecimento (MAPA) e executadas, em Santa Catarina, pela Companhia Integrada de Desenvolvimento Agrícola de Santa Catarina (CIDASC). O objetivo deste trabalho foi analisar e divulgar dados referentes à movimentação de aves oriundas de produções comerciais de Santa Catarina, considerando o trânsito intra e interestadual ocorrido durante o ano de 2017. Para tal, informações referentes a este período foram obtidas no banco de dados da CIDASC. Os resultados demonstraram que foram movimentados, em média por mês, no trânsito intraestadual, no período avaliado, 136.210.943 aves, sendo 72.919.263 para abate, 62.710.860 para engorda e 580.820 para reprodução. Os municípios catarinenses que mais movimentaram aves (origem) foram Videira, Herval d`Oeste, Faxinal dos Guedes, Chapecó e Araranguá, e os municípios que mais receberam aves (destino) foram Capinzal, Videira, Concórdia, Chapecó e Seara. Análises referentes ao trânsito interestadual demonstraram que foram movimentados mensalmente, para fora de Santa Catarina, em média, no período avaliado, 20.514.668 aves, sendo 1.641.304 para abate, 18.841.244 para engorda e 32.120 para reprodução. Os principais destinos das aves catarinenses são Paraná, Rio Grande do Sul e São Paulo. Nossos resultados demonstram a importância da avicultura na região Oeste do Estado e a ocorrência de um intenso trânsito de aves no Estado, sendo o transporte intraestadual responsável por 90,74% desta movimentação. Devido aos riscos sanitários que o transporte de animais representa, esforços para o controle deste transporte devem ser continuamente realizados. Palavras chaves: aves de produção, movimentação intraestadual, movimentação interestadual Evaluation of inter and intra-seasonal movement of commercial birds produced in Santa Catarina in 2017 ABSTRACT. Animal transit is one of the main ways of disease dissemination. Because several diseases can threaten and impact the productive chain, they are targeted by control programs by the Official Health Protection Service of each country worldwide. With the Article History:

The presence of mycotoxins in swine feed can trigger off serious problems. Besides influencing th... more The presence of mycotoxins in swine feed can trigger off serious problems. Besides influencing the quality and quantity of semen produced, it can also cause weight loss, as well as alterations of the immune and reproductive systems. This study reports on an assessment of male swine reproductive parameters over the years 2010 to 2011, at a small rural property in Santa Catarina State, Brazil, seeking to correlate them with possible contamination of the swine’s diet by different mycotoxins. Out of a total of 12 samples of feed collected between August 2010 and February 2011, 58.3% were found to be contaminated with fumonisins (FBs). Only one sample of feed was contaminated by zearalenone (ZON) (64.10 μg kg -1 ). No contamination by aflatoxins (AFLs), ochratoxin A (OTA) and sterigmatocystin (EST) was detected. 75 swine semen samples were collected and analysed in relation to reproductive parameters (sperm motility, volume and density). The findings were correlated to the levels of myco...

Applied Clay Science, 2016

Journal of Hazardous Materials

Bentonites are commonly used as feed additives to reduce the bioavailability and thus the toxicit... more Bentonites are commonly used as feed additives to reduce the bioavailability and thus the toxicity of aflatoxins by adsorbing the toxins in the gastrointestinal tract. Aflatoxins are particular harmful mycotoxins mainly found in areas with hot and humid climates. They occur in food and feedstuff as a result of fungal contamination before and after harvest. The aim of this study was to modify Brazilian bentonite clay by incorporation of zinc (Zn) ions in order to increase the adsorption capacity and consequently reduce the toxicity of aflatoxins. The significance of Zn intercalating conditions such as concentration, temperature and reaction time were investigated. Our results showed that the Zn treatment of the bentonite increased the aflatoxin B(AFB) adsorption and that Zn concentration had a negative effect. Indeed, temperature and time had no significant effect in the binding capacity. The modified bentonite (Zn-Bent1) was not cytotoxic to either fibroblasts (3T3) nor epithelial colorectal adenocarcinoma cells (Caco-2) cell lines. Interestingly, Zn-Bent1 has higher protective effect against AFBinduced cytotoxicity than the unmodified bentonite. In conclusion, the Zn modified bentonite, Zn-Bent1, represent an improved tool to prevent aflatoxicosis in animals fed on AFBcontaminated feed.

Neurochemical Research, Mar 1, 2010

Flavonoids are naturally occurring polyphenolic compounds that are present in a variety of fruits... more Flavonoids are naturally occurring polyphenolic compounds that are present in a variety of fruits, vegetables, cereals, tea, and wine, and are the most abundant antioxidants in the human diet. Evidence suggests that these phytochemicals might have an impact on brain pathology and aging; however, neither their mechanisms of action nor their cell targets are completely known. In the mature mammalian brain, astroglia constitute nearly half of the total cells, providing structural, metabolic, and trophic support for neurons. During the past few years, increasing knowledge of these cells has indicated that astrocytes are pivotal characters in neurodegenerative diseases and brain injury. Most of the physiological benefits of flavonoids are generally thought to be due to their antioxidant and freeradical scavenging effects; however, emerging evidence has supported the hypothesis that their mechanism of action might go beyond these properties. In this review, we focus on astrocytes as targets for flavonoids and their implications in brain development, neuroprotection, and glial tumor formation. Finally, we will briefly discuss the emerging view of astrocytes as essential characters in neurodegenerative diseases, and how a better understanding of the action of flavonoids might open new avenues to develop therapeutic approaches to these pathologies.

Journal of Pharmacy and Pharmacology, 2015

Activation of cannabinoid receptors reduces cell death in R1 ES cells EBs were incubated for 2 da... more Activation of cannabinoid receptors reduces cell death in R1 ES cells EBs were incubated for 2 days with vehicle (control) (a) or WIN, a cannabinoid receptor agonist (b). Following treatment, EBs were fixed and subjected to a fluorescent TUNEL assay. Quantification of cell death by the TUNEL assay relative to the total DAPI stained cells. Results normalized with control values. n53 experiments, with five EBs each. Significantly different from the control, ***P,0.001.

Applied Clay Science, 2015

ABSTRACT Bentonites, which have been shown to be good mycotoxin adsorbents, have a wide range of ... more ABSTRACT Bentonites, which have been shown to be good mycotoxin adsorbents, have a wide range of biochemical and industrial uses. Although many in vitro studies have shown the effects of bentonite on a variety of cell types, there is no comparable information regarding its effects on neural crest (NC) stem cells. The NC is a collection of multipotent and oligopotent progenitors endowed with both neural and mesenchymal potentials. NC cells originated at trunk level can become peripheral nervous system cells, melanocytes and smooth muscle cells. For this reason, the NC is considered an ideal model for studying the role of different substances on cell differentiation, survival and growth. We have recently shown that aflatoxin B1 (AFB1) decreases the viability and reduces the population of NC cells. In the present work, we have characterized bentonite extracted from the South of Brazil and investigated its effects on NC differentiation and survival, when challenged with AFB1. The results have highlighted the high content of SiO2, Fe2O3 and K2O present in bentonite, with content of MnO, MgO, CaO, Na2O, TiO2 and P2O5 lower than 1%. These results have also shown that bentonite at 0.6 mg/mL is not toxic to NC cells in culture. Furthermore, bentonite increased the viability of NC cells previously treated with AFB1 without affecting cell differentiation. The evidence suggests that AFB1 interacted with bentonite by occupying the interlayer spaces, which might have contributed to a reduction of AFB1 availability and, consequently, a reduction in NC damage and an increase in the total number of cells. Our study contributes to a better understanding of the effects of clay minerals on the NC and may help to create new, more effective pharmacological applications to reduce or eliminate the toxic effects of AFB1.

Journal of Neuroscience Research, 2012

Sphingosine 1-phosphate (S1P) is a bioactive signaling lysophospholipid. Effects of S1P on prolif... more Sphingosine 1-phosphate (S1P) is a bioactive signaling lysophospholipid. Effects of S1P on proliferation, survival, migration, and differentiation have already been described; however, its role as a mediator of interactions between neurons and glial cells has been poorly explored. Here we describe effects of S1P, via the activation of its receptors in astrocytes, on the differentiation of neural progenitor cells (NPC) derived from either embryonic stem cells or the developing cerebral cortex. S1P added directly to NPC induced their differentiation, but S1P-primed astrocytes were able to promote even more pronounced changes in maturation, neurite outgrowth, and arborization in NPC. An increase in laminin by astrocytes was observed after S1P treatment. The effects of S1P-primed astrocytes on neural precursor cells were abrogated by antibodies against laminin. Together, our data indicate that S1P-treated astrocytes are able to induce neuronal differentiation of NPC by increasing the levels of laminin. These results implicate S1P signaling pathways as new targets for understanding neuroglial interactions within the central nervous system. V V C 2012 Wiley Periodicals, Inc.

Neurochemical research, 2011

Flavonoids comprise the most common group of plant polyphenols and provide much of the flavor and... more Flavonoids comprise the most common group of plant polyphenols and provide much of the flavor and color to fruits and vegetables. More than 5,000 different flavonoids have been described. The biological activities of flavonoids cover a very broad spectrum, from anticancer and antibacterial activities to inhibition of bone reabsorption and neuroprotection effect. Although emerging evidence suggests that flavonoids have an important role on brain development, little is known about their mechanisms of action. In the present work, we performed a screening of flavonoid actions by analyzing the effects of these substances (hesperidin and rutin) on neural progenitors and neuronal morphogenesis in vitro. We demonstrated that treatment of neural progenitors with the flavonoid hesperidin enhanced neuronal population as revealed by an 80% increase in the number of β-tubulin III cells. This effect was mainly due to modulation of neuronal progenitor survival. Pools of astrocyte and oligodendrocy...

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, 2012

Flavonoids are polyphenolic compounds that are integral components of the human diet, universally... more Flavonoids are polyphenolic compounds that are integral components of the human diet, universally present as constituents of fruits and vegetables as well as plant-derived foods and beverages such as oil, tea, and red wine. The biological activities of flavonoids cover a very broad spectrum, from anticancer and antibacterial activities to inhibition of bone reabsorption and modulation of inflammatory response. Although emerging evidence has suggested that flavonoids might have an impact on brain pathology and aging, their role as a mediator in interactions between neurons and glial cells has been poorly explored. In the present work, we have performed a screening of flavonoid actions by analyzing the effects of hesperidin, quercetin and rutin on murine cerebral cortex astrocytes and neural progenitors. Treatment of astrocytes with flavonoids did not interfere with cell viability and proliferation. However a culture of neural progenitors with conditioned medium from hesperidin treate...

Cell Biology International, 2010

ESCs (embryonic stem cells) are potentially able to replace damaged cells in animal models of neu... more ESCs (embryonic stem cells) are potentially able to replace damaged cells in animal models of neural pathologies such as Parkinson's disease, stroke and spinal cord lesions. Nevertheless, many issues remain unsolved regarding optimal culturing procedures for these cells. For instance, on their path to differentiation in vitro, which usually involves the formation of EBs (embryoid bodies), they may present chromosomal instability, loss of pluripotency or simply die. Therefore, finding strategies to increase the survival of cells within EBs is of great interest. Cannabinoid receptors have many roles in the physiology of the adult body, but little is known about their role in the biology of ESCs. Herein, we investigated how two cannabinoid receptors, CB1 and CB2, may affect the outcome of ESCs aggregated as EBs. RT-PCR (reverse transcriptase-PCR) revealed that EBs expressed both CB1 and CB2 receptors. Aggregation of ESCs into EBs followed by 2-day incubation with a CB1/CB2 agonist reduced cell death by approximately 45%, which was reversed by a CB1 antagonist. A specific CB2 agonist also reduced cell death by approximately 20%. These data indicate that both cannabinoid receptors, CB1 and CB2, are involved in reducing cell death in EBs mediated by exogenous cannabinoids. No increase in proliferation, neural differentiation or changes in chromosomal stability was observed. This study indicates that cannabinoid signalling is functionally implicated in the biology of differentiating ESCs, being the first to show that activation of cannabinoid receptors is able to increase cell viability via reduction of cell death rate in EBs.

Applied Clay Science, 2015

Cell Biology International, 2013

The neural crest (NC) corresponds to a collection of multipotent and oligopotent progenitors endo... more The neural crest (NC) corresponds to a collection of multipotent and oligopotent progenitors endowed with both neural and mesenchymal potentials. The derivatives of the NC at trunk level include neurons and glial cells of the peripheral nervous system. Despite the well-known influence of aflatoxins on the development of cancer, the issue of whether they also influence NC cells has not been yet addressed. In the present work, we have investigated the effects of aflatoxin B 1 on quail NC cells and the concomitant effects of the flavonoid hesperidin associated with this mycotoxin. We show for the first time that aflatoxin B 1 decreases the viability and the total number of glial and neuronal cells/field, although their proportions in relation to the total number of cells were not altered. Therefore, aflatoxin has no effect on NC differentiation. However, this compound was able to reduce NC proliferation and NC survival. Furthermore, the co-administration of hesperidin, a well-known polyphenolic protector of cell death, partially prevented the effect of aflatoxin B 1 . Taken together, our results demonstrate that aflatoxin B 1 is toxic to NC cells, an effect partially prevented by the flavonoid hesperidin. This study may contribute to the understanding of the effects of these compounds during early embryonic development and offer potentially more assertive diets and treatments for pregnant animals.

Cell and Tissue Research, 2012

The neural crest (NC) corresponds to a collection of multipotent and oligopotent progenitors endo... more The neural crest (NC) corresponds to a collection of multipotent and oligopotent progenitors endowed with both neural and mesenchymal potentials. The derivatives of the NC at trunk level include neurons and glial cells of the peripheral nervous system in addition to melanocytes, smooth muscle cells and some endocrine cells. Environmental factors control the fate decisions of NC cells. Despite the well-known influence of flavonoids on the central nervous system, the issue of whether they also influence NC cells has not been yet addressed. Flavonoids are polyphenolic compounds that are integral components of the human diet. The biological activities of these compounds cover a very broad spectrum, from anticancer and antibacterial activities to inhibition of bone reabsorption and modulation of inflammatory response. In the present work, we have investigated the actions of the flavonoids hesperidin, rutin and quercetin on NC cells of quail, in vitro. We show for the first time, that hesperidin and rutin increase the viability of trunk NC cells in culture, without affecting cell differentiation and proliferation. The molecular mechanism of this action is dependent on ERK2 and PI3K pathways. Quercetin had no effect on NC progenitors. Taken together, these results suggest that flavonoids hesperidin and rutin increase NC cell survival, which may be useful against the toxicity of some chemicals during embryonic development.

Materials science & engineering. C, Materials for biological applications, 2015

Bentonites are clays that highly adsorb aflatoxin B1 (AFB1) and, therefore, protect human and ani... more Bentonites are clays that highly adsorb aflatoxin B1 (AFB1) and, therefore, protect human and animal cells from damage. We have recently demonstrated that bentonite protects the neural crest (NC) stem cells from the toxicity of AFB1. Its protective effects are due to the physico-chemical properties and chemical composition altered by heat treatment. The aim of this study is to prepare and characterize the natural and thermal treatments (125 to 1000°C) of bentonite from Criciúma, Santa Catarina, Brazil and to investigate their effects in the AFB1 adsorption and in NC cell viability after challenging with AFB1. The displacement of water and mineralogical phases transformations were observed after the thermal treatments. Kaolinite disappeared at 500°C and muscovite and montmorillonite at 1000°C. Slight changes in morphology, chemical composition, and density of bentonite were observed. The adsorptive capacity of the bentonite particles progressively reduced with the increase in tempera...

v. 28, n. 4 (2015) by Jader Nones

A crista neural (CN) corresponde a um conjunto de células progenitoras multi e oligopotentes dota... more A crista neural (CN) corresponde a um conjunto de células progenitoras multi e oligopotentes dotadas com potenciais neural e mesenquimal. Os derivados da CN incluem neurônios e células gliais do sistema nervoso periférico, melanócitos, células da musculatura lisa e algumas células endócrinas. No presente trabalho, investigamos pela primeira vez a influência da aflatoxina B1 (AFB1) e do flavonoide rutina na sobrevivência e proliferação da CN e de melanócitos derivados deste tipo celular. Para tal, culturas de células da CN de codornas foram tratadas com AFB1 (30 μM) e/ou rutina (20 μM) durante seis dias. A viabilidade celular foi avaliada por análises de MTT e azul de tripan e a proliferação celular através de marcação com BrdU. Melanócitos foram identificados com uso do marcador celular específico MelEM. O tratamento com a AFB1 diminuiu a viabilidade e proliferação das células da CN. O número total de células MelEM-positivas foi também reduzido após este tratamento, efeito parcialmente revertido através da adição de rutina. No entanto, uma melhor compreensão referente aos mecanismos envolvidos nas interações entre AFB1 e rutina precisarão ser realizados.

Pubvet, Jul 1, 2018

Applied Clay Science, 2016

Journal of Hazardous Materials

Neurochemical Research, Mar 1, 2010

Journal of Pharmacy and Pharmacology, 2015

Applied Clay Science, 2015

Journal of Neuroscience Research, 2012

Neurochemical research, 2011

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, 2012

Cell Biology International, 2010

Applied Clay Science, 2015

Cell Biology International, 2013

Cell and Tissue Research, 2012

The neural crest (NC) corresponds to a collection of multipotent and oligopotent progenitors endo... more The neural crest (NC) corresponds to a collection of multipotent and oligopotent progenitors endowed with both neural and mesenchymal potentials. The derivatives of the NC at trunk level include neurons and glial cells of the peripheral nervous system in addition to melanocytes, smooth muscle cells and some endocrine cells. Environmental factors control the fate decisions of NC cells. Despite the well-known influence of flavonoids on the central nervous system, the issue of whether they also influence NC cells has not been yet addressed. Flavonoids are polyphenolic compounds that are integral components of the human diet. The biological activities of these compounds cover a very broad spectrum, from anticancer and antibacterial activities to inhibition of bone reabsorption and modulation of inflammatory response. In the present work, we have investigated the actions of the flavonoids hesperidin, rutin and quercetin on NC cells of quail, in vitro. We show for the first time, that hesperidin and rutin increase the viability of trunk NC cells in culture, without affecting cell differentiation and proliferation. The molecular mechanism of this action is dependent on ERK2 and PI3K pathways. Quercetin had no effect on NC progenitors. Taken together, these results suggest that flavonoids hesperidin and rutin increase NC cell survival, which may be useful against the toxicity of some chemicals during embryonic development.

Materials science & engineering. C, Materials for biological applications, 2015