Rubayet Jahan - Academia.edu (original) (raw)

Papers by Rubayet Jahan

Annals of Agricultural Sciences

Cell and Tissue Research, 2021

Huntingtin-associated protein 1 (HAP1) is a neural huntingtin interactor and being considered as ... more Huntingtin-associated protein 1 (HAP1) is a neural huntingtin interactor and being considered as a core molecule of stigmoid body (STB). Brain/spinal cord regions with abundant STB/HAP1 expression are usually spared from neurodegeneration in stress/disease conditions, whereas the regions with little STB/HAP1 expression are always neurodegenerative targets. The enteric nervous system (ENS) can act as a potential portal for pathogenesis of neurodegenerative disorders. However, ENS is also a neurodegenerative target in these disorders. To date, the expression of HAP1 and its neurochemical characterization have never been examined there. In the current study, we determined the expression of HAP1 in the ENS of adult mice and characterized the morphological relationships of HAP1-immunoreactive (ir) cells with the markers of motor neurons, sensory neurons, and interneurons in the myenteric plexus using Western blotting and light/fluorescence microscopy. HAP1-immunoreaction was present in both myenteric and submucosal plexuses of ENS. Most of the HAP1-ir neurons exhibited STB in their cytoplasm. In myenteric plexus, a large number of calretinin, calbindin, NOS, VIP, ChAT, SP, somatostatin, and TH-ir neurons showed HAP1-immunoreactivity. In contrast, most of the CGRP-ir neurons were devoid of HAP1-immunoreactivity. Our current study is the first to clarify that HAP1 is highly expressed in excitatory motor neurons, inhibitory motor neurons, and interneurons but almost absent in sensory neurons in myenteric plexus. These suggest that STB/HAP1-ir neurons are mostly Dogiel type I neurons. Due to lack of putative STB/HAP1 protectivity, the sensory neurons (Dogiel type II) might be more vulnerable to neurodegeneration than STB/HAP1-expressing motoneurons/interneurons (Dogiel type I) in myenteric plexus.

Brain Sciences, 2021

Adolescence is the critical postnatal stage for the action of androgen in multiple brain regions.... more Adolescence is the critical postnatal stage for the action of androgen in multiple brain regions. Androgens can regulate the learning/memory functions in the brain. It is known that the inhibitory avoidance test can evaluate emotional memory and is believed to be dependent largely on the amygdala and hippocampus. However, the effects of androgen on inhibitory avoidance memory have never been reported in adolescent male rats. In the present study, the effects of androgen on inhibitory avoidance memory and on androgen receptor (AR)-immunoreactivity in the amygdala and hippocampus were studied using behavioral analysis, Western blotting and immunohistochemistry in sham-operated, orchiectomized, orchiectomized + testosterone or orchiectomized + dihydrotestosterone-administered male adolescent rats. Orchiectomized rats showed significantly reduced time spent in the illuminated box after 30 min (test 1) or 24 h (test 2) of electrical foot-shock (training) and reduced AR-immunoreactivity i...

IBRO Reports, 2020

Huntingtin-associated protein 1 (HAP1) is a polyglutamine (polyQ) length-dependent interactor wit... more Huntingtin-associated protein 1 (HAP1) is a polyglutamine (polyQ) length-dependent interactor with causal agents in several neurodegenerative diseases and has been regarded as a protective factor against neurodegeneration. In normal rodent brain and spinal cord, HAP1 is abundantly expressed in the areas that are spared from neurodegeneration while those areas with little HAP1 are frequent targets of neurodegeneration. We have recently showed that HAP1 is highly expressed in the spinal dorsal horn and may participate in modification/ protection of certain sensory functions. Neurons in the dorsal root ganglia (DRG) transmits sensory stimuli from periphery to spinal cord/brain stem. Nevertheless, to date HAP1 expression in DRG remains unreported. In this study, the expression of HAP1 in cervical, thoracic, lumbar and sacral DRG in adult male mice and its relationships with different chemical markers for sensory neurons were examined using Western blot and immunohistochemistry. HAP1-immunoreactivity was detected in the cytoplasm of DRG neurons, and the percentage of HAP1-immunoreactive (ir) DRG neurons was ranged between 28-31 %. HAP1-immunoreactivity was comparatively more in the small cells (47-58 %) and medium cells (40-44 %) than that in the large cells (9-11 %). Double-immunostaining for HAP1 and markers for nociceptive or mechanoreceptive neurons showed that about 70-80 % of CGRP-, SP-, CB-, NOS-, TRPV1-, CR-and PV-ir neurons expressed HAP1. In contrast, HAP1 was completely lacking in TH-ir neurons. Our current study is the first to clarify that HAP1 is highly expressed in nociceptive/proprioceptive neurons but absent in light-touch-sensitive TH neurons, suggesting the potential importance of HAP1 in pain transduction and proprioception.

Neuroscience, 2020

affects the dynamics of intrinsic plasticity of pyramidal neurons in the CA1 hippocampal subfield... more affects the dynamics of intrinsic plasticity of pyramidal neurons in the CA1 hippocampal subfield in adolescent male rats, Neuroscience (2020),

Saudi Journal of Biological Sciences, 2021

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Asian Journal of Medical and Biological Research, 2018

Native chickens of Bangladesh are scavenging in nature. In our previous studies, we have histolog... more Native chickens of Bangladesh are scavenging in nature. In our previous studies, we have histologically and immunohistochemically examined the ontogeny of lymphoid organs of native chickens of Bangladesh in prenatal stages. To date, the study on gross anatomical development of prenatal lymphoid organs is lacking. In the present study, detailed macro-anatomical development of prenatal lymphoid organs of native chickens were examined during different embryonic stages of development, which was conducted in the laboratory of the department of Anatomy and Histology, Bangladesh Agricultural University. In the present study, thymus was first identified by the 10 days of incubation (ED 10) as a chain at the both side of the neck. Bursa of Fabricius and spleen were also first found to develop at ED 10. While comparing with the prenatal development of lymphoid organs of native chickens with that of previously studied hybrid chickens, the native chickens’ lymphoid organs were found to develop ...

Journal of Advanced Biotechnology and Experimental Therapeutics, 2018

Johann Jacob Harder first described the Harderian gland in 1694 in deer. It is found in most terr... more Johann Jacob Harder first described the Harderian gland in 1694 in deer. It is found in most terrestrial animals and is located within the variable aspects of the orbit. It is believed that this gland is involved in diverse functions. Among these, it has been held to be a site of immune response, a source of thermoregulatory lipids and pheromones, act as photoprotective organ as well as part of a retinal-pineal axis. In birds, this glad was reported first in sparrow in 1918. The Harderian gland is covered by capsule and the connective tissue septa that divide the gland into numerous unequal-sized lobes and lobules. Plasma cells are found in the interacinar space and the lumina of lobules. The recent studies suggest that the Harderian gland act as an immunopotent organ in birds, and that the gland in scavenging birds contains more immunoglobulin-containing plasma cells due to their scavenging nature. Moreover, this gland shows considerable species/strain differences in terms of macro anatomy, microanatomy as well as in the dynamics of immunoglobulin-containing plasma cells among different birds. In this review, these species and strain differences are discussed based on recent studies and several goals of future research are identified.

Neuroscience, 2018

Huntingtin-associated protein 1 (HAP1) is a neural interactor of huntingtin in Huntington's disea... more Huntingtin-associated protein 1 (HAP1) is a neural interactor of huntingtin in Huntington's disease, and interacts with gene products in a number of other neurodegenerative diseases. In normal brains, HAP1 is expressed abundantly in the hypothalamus and limbic-associated regions. These areas tend to be spared from neurodegeneration while those with little HAP1 are frequently neurodegenerative targets, suggesting its role as a protective factor against apoptosis. In light of the relationship between neurodegenerative diseases and deterioration of higher nervous activity, it is important to definitively clarify HAP1 expression in a cognitively important brain region, the retrosplenial-retrohippocampal area. Here, HAP1 expression was evaluated immunohistochemically over the retrosplenial cortex, the subicular complex, and the entorhinal and perirhinal cortices. HAP1-immunoreactive (ir) cells were classified into five discrete groups: (1) a distinct retrosplenial cell cluster exclusive to the superficial layers of the granular cortex, (2) a conspicuous, thin line of cells in layers IV/V of the ''subiculum-backing cortex," (3) a group of highly immunoreactive cells associated with the medial entorhinal-subicular corner, (4) pericallosal cells just below layer VI and adjacent to the white matter, and (5) other sporadic, widely disseminated HAP1-immunoreactive cells. HAP1 was found to be the first marker for the combined subiculum-backing cortex and a precise marker for several subfields in the retrosplenial-retrohippocampal area, verified through comparative staining with other neurochemicals. HAP1 may play an important role in protecting these cortical structures and functions for higher nervous activity by increasing the threshold to neurodegeneration and decreasing vulnerability to stress or aging.

Neuroscience, Jan 6, 2017

Huntingtin-associated protein 1 (HAP1) is a neuronal interactor with causatively polyglutamine (p... more Huntingtin-associated protein 1 (HAP1) is a neuronal interactor with causatively polyglutamine (polyQ)-expanded huntingtin in Huntington's disease and also associated with pathologically polyQ-expanded androgen receptor (AR) in spinobulbar muscular atrophy (SBMA), being considered as a protective factor against neurodegenerative apoptosis. In normal brains, it is abundantly expressed particularly in the limbic-hypothalamic regions that tend to be spared from neurodegeneration, whereas the areas with little HAP1 expression, including the striatum, thalamus, cerebral neocortex and cerebellum, are targets in several neurodegenerative diseases. While the spinal cord is another major neurodegenerative target, HAP1-immunoreactive (ir) structures have yet to be determined there. In the current study, HAP1 expression was immunohistochemically evaluated in light and electron microscopy through the cervical, thoracic, lumbar, and sacral spinal cords of the adult male rat. Our results show...

Bangladesh Journal of Veterinary Medicine, 2008

A total of 24, six weeks old mice were used to study the body weight and haemato-biochemical chan... more A total of 24, six weeks old mice were used to study the body weight and haemato-biochemical changes following administering of different haematinics (CuSO4, FeSO4 and Vitamin B12). The experiment was performed in Physiology laboratory, Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, from February to March 2006. They were randomly assigned to one of four equal groups (n = 6). In addition to normal rat pellets Group A was supplemented with CuSO4 @ 0.6mg/mice/day orally, Group B with FeSO4 @ 1.2mg/mice/day orally, and Group C with Vitamin B12 (Cytamin®, Glaxo) @ 0.004mg/mice intramuscularly at every 7 days. Group D was considered as control and was also supplemented with rat pellets. Increased body weight was observed in all haematinic treated mice but group B had a significant (p < 0.05) higher weight gain compared to the control. TEC and PCV increased significantly (p < 0.05) in all the treatment groups compared to the ...

Bangladesh Journal of Veterinary Medicine, 2008

In the present study, five Black Bengal goats (Capra hircus) were used to investigate the bones o... more In the present study, five Black Bengal goats (Capra hircus) were used to investigate the bones of fore limb during January to June 2006. It was observed topographically that the scapula was more or less similar to other ruminant animal topographically with exception that, the presence of very short coracoid process, more or less oval shaped glenoid cavity and more extensive subscapular fossa. The humerus of adult Black Bengal goat was 12.06 ± 0.27cm in length. The diameter of shaft at the level of nutrient foramen was 4.24 ± 0.05 cm. The breadth of humerus was 1.66 ± 0.06 cm. The deltoid tuberosity was less prominent and there was shallow radial and olecranon fossa. The radius of adult Black Bengal goat was 11.12 ± 0.23 cm in length. The diameter of shaft of radius-ulna just below the interosseous space was 3.86 ± 0.12cm. The total length of ulna of adult Black Bengal goat was 14.20 ± 0.20 cm. The interosseous space was found very narrow in the present study. Six carpal bones...

The Journal of Poultry Science, 2008

The aims of this study was to find out the histological di erences of the mucosa and comparative ... more The aims of this study was to find out the histological di erences of the mucosa and comparative analysis of Ig-containing plasma cells among the di erent segments of the gastrointestinal tract of broiler and native chickens of Bangladesh. The conventional histological study revealed that the lining epithelium of the proximal segments (esophagus, crop, and proventriculus) were thicker in the broiler. The esophageal glands were more in the broiler than the native chickens. The villi of the duodenum, jejunum and ileum were slender and longer in the broiler in comparison to the native chickens. The number of goblet cells in the duodenum, jejunum, and ileum was more in the native chickens than the broiler. The indirect immunohistochemistry revealed that very few immunoglobulin (Ig)-containing plasma cells were present in the epithelium and lamina propria of esophagus, crop, and proventriculus of the broiler and native chickens. The frequency of the population of these cells were abundantly located in the lamina propria, around the intestinal gland and in the core of the villi from duodenum to ileum of broiler and native chickens. The intraepithelial IgA-containing epithelium was observed only in the epithelium of the native chickens. Segmental variation of Ig-containing plasma cells was noticed in these two strains of chickens. The IgA-, IgG-, and IgM-containing plasma cells were significantly more in the most of the segments of the small intestine of the native chickens. This suggested that besides the existence of histological variation in the gastrointestinal tract of broiler and native chickens, the Ig-containing plasma cells were significantly more in the di erent segments of the digestive tract of native chickens possible due to their scavenging. : broiler, gastrointestinal tract, immunoglobulin cells, mucosa, native chickens (MALT) supply lymphocytes and plasma cells that con-cells, macrophages, dendritic cells and non-professional tribute to the local immune system (McDermott and antigen presenting cells (APCs) (McDonald and Spencer,). These cells principally developed and mobilize from "Gut Associated Lymphoid Tissue (GALT)", which may include esophageal tonsils, Meckel's diverticulum, Peyer's patches, cecal tonsils, and bursa of Fabricius (Arai). The plasma cells were the cells of the immune system that secrete large amounts of antibodies

Bangladesh Journal of Veterinary Medicine, 2014

The present research was designed to study the histological and immunohistochemical changes of ly... more The present research was designed to study the histological and immunohistochemical changes of lymphoid organs (bursa of Fabricius and cecal tonsil) and in the ileum (representative mucosal organ) of broiler chickens after immunization with Gumboro vaccine. Two groups (n=24) of Cobb-500 broiler chickens were reared in a same environment A mild vaccine (BUR® 706) followed by a intermediate (Nobilis® Gumboro 228 E) type vaccine was administered for immunization of chickens and samples were collected at 7 days interval for up to 32 days of age. The conventional histological structure of all three organs of the present study was similar to the earlier reports. In addition, in the bursa of Fabricius, the population of lymphocytes and the size of the follicles were found to increase in vaccinated chickens than the control chickens. The Igs positive cells (IgA, IgG and IgM) were distributed principally beneath the capsule, around the follicles and in the cortex and medulla. The frequencies...

International Journal of Veterinary Science and Medicine, 2013

The first appearance, distribution and frequency of immunoglobulins (Igs)-positive lymphocytes we... more The first appearance, distribution and frequency of immunoglobulins (Igs)-positive lymphocytes were investigated in the lymphoid organs of native chicken's embryos from embryonic day (ED) 8 to ED 20. The tissues from the lymphoid organs were dehydrated in alcohol, cleared in xylene, embedded in different grades of paraffin and 6-micron thick sections were immunostained by the indirect immunoperoxidase method using antichicken immunoglobulins. IgM-positive lymphocytes were first identified in the follicles of bursa of Fabricius at ED 10, in the white pulp of the spleen at ED 14 and in the lamina propria of the cecal tonsil at ED 20. Their frequencies of populations were statistically significant from ED 14 to ED 20. IgG-positive lymphocytes were first appeared in the bursa of Fabricius and spleen at ED 20. In the bursa IgG-positive lymphocytes were located in the medulla and cortical part of the follicles, whereas, in the spleen these immune cells were located around the white pulp. IgA-positive lymphocytes were not observed in any of the developing lymphoid organs of the present study. When the data for bursa of Fabricius, spleen,

Pakistan Veterinary …, 2011

Native chickens (Gallus domesticus) of Bangladesh are scavenging in nature. Data regarding morpho... more Native chickens (Gallus domesticus) of Bangladesh are scavenging in nature. Data regarding morphology of lymphoid organs in prenatal stages are lacking. H&E staining method was performed to study the development of the bursa of Fabricius, thymus, spleen and cecal tonsil from embryonic day (ED) 10 to 20. The budding of thymus was seen on ED 10. At ED 12, fiber network of thymus was formed to create a basement of cells of thymus and on ED 14 these cells began to organize to form cortex and medulla. But the cortex and medulla of thymus could not be differentiated before ED 20. The plicae of bursa Fabricius started to develop on ED 10. From ED 12 the plicae became shorter and wider to form bursal follicles and these follicles were clearly organized into cortex and medulla on ED 20. At ED 10, very thin capsule was seen in embryonic spleen. During ED 12, only a few white pulps were observed, while on ED 14, purple colored white pulp and pinkish red pulp were easily visible. At ED 20, the thickness of capsule was increased and pulps were more distinguishable. All the lymphoid organs showed major development during the later incubation period, indicating that the immune system in that period is being prepared to face the scavenging environment after hatching.

Annals of Agricultural Sciences

Cell and Tissue Research, 2021

Huntingtin-associated protein 1 (HAP1) is a neural huntingtin interactor and being considered as ... more Huntingtin-associated protein 1 (HAP1) is a neural huntingtin interactor and being considered as a core molecule of stigmoid body (STB). Brain/spinal cord regions with abundant STB/HAP1 expression are usually spared from neurodegeneration in stress/disease conditions, whereas the regions with little STB/HAP1 expression are always neurodegenerative targets. The enteric nervous system (ENS) can act as a potential portal for pathogenesis of neurodegenerative disorders. However, ENS is also a neurodegenerative target in these disorders. To date, the expression of HAP1 and its neurochemical characterization have never been examined there. In the current study, we determined the expression of HAP1 in the ENS of adult mice and characterized the morphological relationships of HAP1-immunoreactive (ir) cells with the markers of motor neurons, sensory neurons, and interneurons in the myenteric plexus using Western blotting and light/fluorescence microscopy. HAP1-immunoreaction was present in both myenteric and submucosal plexuses of ENS. Most of the HAP1-ir neurons exhibited STB in their cytoplasm. In myenteric plexus, a large number of calretinin, calbindin, NOS, VIP, ChAT, SP, somatostatin, and TH-ir neurons showed HAP1-immunoreactivity. In contrast, most of the CGRP-ir neurons were devoid of HAP1-immunoreactivity. Our current study is the first to clarify that HAP1 is highly expressed in excitatory motor neurons, inhibitory motor neurons, and interneurons but almost absent in sensory neurons in myenteric plexus. These suggest that STB/HAP1-ir neurons are mostly Dogiel type I neurons. Due to lack of putative STB/HAP1 protectivity, the sensory neurons (Dogiel type II) might be more vulnerable to neurodegeneration than STB/HAP1-expressing motoneurons/interneurons (Dogiel type I) in myenteric plexus.

Brain Sciences, 2021

Adolescence is the critical postnatal stage for the action of androgen in multiple brain regions.... more Adolescence is the critical postnatal stage for the action of androgen in multiple brain regions. Androgens can regulate the learning/memory functions in the brain. It is known that the inhibitory avoidance test can evaluate emotional memory and is believed to be dependent largely on the amygdala and hippocampus. However, the effects of androgen on inhibitory avoidance memory have never been reported in adolescent male rats. In the present study, the effects of androgen on inhibitory avoidance memory and on androgen receptor (AR)-immunoreactivity in the amygdala and hippocampus were studied using behavioral analysis, Western blotting and immunohistochemistry in sham-operated, orchiectomized, orchiectomized + testosterone or orchiectomized + dihydrotestosterone-administered male adolescent rats. Orchiectomized rats showed significantly reduced time spent in the illuminated box after 30 min (test 1) or 24 h (test 2) of electrical foot-shock (training) and reduced AR-immunoreactivity i...

IBRO Reports, 2020

Huntingtin-associated protein 1 (HAP1) is a polyglutamine (polyQ) length-dependent interactor wit... more Huntingtin-associated protein 1 (HAP1) is a polyglutamine (polyQ) length-dependent interactor with causal agents in several neurodegenerative diseases and has been regarded as a protective factor against neurodegeneration. In normal rodent brain and spinal cord, HAP1 is abundantly expressed in the areas that are spared from neurodegeneration while those areas with little HAP1 are frequent targets of neurodegeneration. We have recently showed that HAP1 is highly expressed in the spinal dorsal horn and may participate in modification/ protection of certain sensory functions. Neurons in the dorsal root ganglia (DRG) transmits sensory stimuli from periphery to spinal cord/brain stem. Nevertheless, to date HAP1 expression in DRG remains unreported. In this study, the expression of HAP1 in cervical, thoracic, lumbar and sacral DRG in adult male mice and its relationships with different chemical markers for sensory neurons were examined using Western blot and immunohistochemistry. HAP1-immunoreactivity was detected in the cytoplasm of DRG neurons, and the percentage of HAP1-immunoreactive (ir) DRG neurons was ranged between 28-31 %. HAP1-immunoreactivity was comparatively more in the small cells (47-58 %) and medium cells (40-44 %) than that in the large cells (9-11 %). Double-immunostaining for HAP1 and markers for nociceptive or mechanoreceptive neurons showed that about 70-80 % of CGRP-, SP-, CB-, NOS-, TRPV1-, CR-and PV-ir neurons expressed HAP1. In contrast, HAP1 was completely lacking in TH-ir neurons. Our current study is the first to clarify that HAP1 is highly expressed in nociceptive/proprioceptive neurons but absent in light-touch-sensitive TH neurons, suggesting the potential importance of HAP1 in pain transduction and proprioception.

Neuroscience, 2020

affects the dynamics of intrinsic plasticity of pyramidal neurons in the CA1 hippocampal subfield... more affects the dynamics of intrinsic plasticity of pyramidal neurons in the CA1 hippocampal subfield in adolescent male rats, Neuroscience (2020),

Saudi Journal of Biological Sciences, 2021

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Asian Journal of Medical and Biological Research, 2018

Native chickens of Bangladesh are scavenging in nature. In our previous studies, we have histolog... more Native chickens of Bangladesh are scavenging in nature. In our previous studies, we have histologically and immunohistochemically examined the ontogeny of lymphoid organs of native chickens of Bangladesh in prenatal stages. To date, the study on gross anatomical development of prenatal lymphoid organs is lacking. In the present study, detailed macro-anatomical development of prenatal lymphoid organs of native chickens were examined during different embryonic stages of development, which was conducted in the laboratory of the department of Anatomy and Histology, Bangladesh Agricultural University. In the present study, thymus was first identified by the 10 days of incubation (ED 10) as a chain at the both side of the neck. Bursa of Fabricius and spleen were also first found to develop at ED 10. While comparing with the prenatal development of lymphoid organs of native chickens with that of previously studied hybrid chickens, the native chickens’ lymphoid organs were found to develop ...

Journal of Advanced Biotechnology and Experimental Therapeutics, 2018

Johann Jacob Harder first described the Harderian gland in 1694 in deer. It is found in most terr... more Johann Jacob Harder first described the Harderian gland in 1694 in deer. It is found in most terrestrial animals and is located within the variable aspects of the orbit. It is believed that this gland is involved in diverse functions. Among these, it has been held to be a site of immune response, a source of thermoregulatory lipids and pheromones, act as photoprotective organ as well as part of a retinal-pineal axis. In birds, this glad was reported first in sparrow in 1918. The Harderian gland is covered by capsule and the connective tissue septa that divide the gland into numerous unequal-sized lobes and lobules. Plasma cells are found in the interacinar space and the lumina of lobules. The recent studies suggest that the Harderian gland act as an immunopotent organ in birds, and that the gland in scavenging birds contains more immunoglobulin-containing plasma cells due to their scavenging nature. Moreover, this gland shows considerable species/strain differences in terms of macro anatomy, microanatomy as well as in the dynamics of immunoglobulin-containing plasma cells among different birds. In this review, these species and strain differences are discussed based on recent studies and several goals of future research are identified.

Neuroscience, 2018

Huntingtin-associated protein 1 (HAP1) is a neural interactor of huntingtin in Huntington's disea... more Huntingtin-associated protein 1 (HAP1) is a neural interactor of huntingtin in Huntington's disease, and interacts with gene products in a number of other neurodegenerative diseases. In normal brains, HAP1 is expressed abundantly in the hypothalamus and limbic-associated regions. These areas tend to be spared from neurodegeneration while those with little HAP1 are frequently neurodegenerative targets, suggesting its role as a protective factor against apoptosis. In light of the relationship between neurodegenerative diseases and deterioration of higher nervous activity, it is important to definitively clarify HAP1 expression in a cognitively important brain region, the retrosplenial-retrohippocampal area. Here, HAP1 expression was evaluated immunohistochemically over the retrosplenial cortex, the subicular complex, and the entorhinal and perirhinal cortices. HAP1-immunoreactive (ir) cells were classified into five discrete groups: (1) a distinct retrosplenial cell cluster exclusive to the superficial layers of the granular cortex, (2) a conspicuous, thin line of cells in layers IV/V of the ''subiculum-backing cortex," (3) a group of highly immunoreactive cells associated with the medial entorhinal-subicular corner, (4) pericallosal cells just below layer VI and adjacent to the white matter, and (5) other sporadic, widely disseminated HAP1-immunoreactive cells. HAP1 was found to be the first marker for the combined subiculum-backing cortex and a precise marker for several subfields in the retrosplenial-retrohippocampal area, verified through comparative staining with other neurochemicals. HAP1 may play an important role in protecting these cortical structures and functions for higher nervous activity by increasing the threshold to neurodegeneration and decreasing vulnerability to stress or aging.

Neuroscience, Jan 6, 2017

Huntingtin-associated protein 1 (HAP1) is a neuronal interactor with causatively polyglutamine (p... more Huntingtin-associated protein 1 (HAP1) is a neuronal interactor with causatively polyglutamine (polyQ)-expanded huntingtin in Huntington's disease and also associated with pathologically polyQ-expanded androgen receptor (AR) in spinobulbar muscular atrophy (SBMA), being considered as a protective factor against neurodegenerative apoptosis. In normal brains, it is abundantly expressed particularly in the limbic-hypothalamic regions that tend to be spared from neurodegeneration, whereas the areas with little HAP1 expression, including the striatum, thalamus, cerebral neocortex and cerebellum, are targets in several neurodegenerative diseases. While the spinal cord is another major neurodegenerative target, HAP1-immunoreactive (ir) structures have yet to be determined there. In the current study, HAP1 expression was immunohistochemically evaluated in light and electron microscopy through the cervical, thoracic, lumbar, and sacral spinal cords of the adult male rat. Our results show...

Bangladesh Journal of Veterinary Medicine, 2008

A total of 24, six weeks old mice were used to study the body weight and haemato-biochemical chan... more A total of 24, six weeks old mice were used to study the body weight and haemato-biochemical changes following administering of different haematinics (CuSO4, FeSO4 and Vitamin B12). The experiment was performed in Physiology laboratory, Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, from February to March 2006. They were randomly assigned to one of four equal groups (n = 6). In addition to normal rat pellets Group A was supplemented with CuSO4 @ 0.6mg/mice/day orally, Group B with FeSO4 @ 1.2mg/mice/day orally, and Group C with Vitamin B12 (Cytamin®, Glaxo) @ 0.004mg/mice intramuscularly at every 7 days. Group D was considered as control and was also supplemented with rat pellets. Increased body weight was observed in all haematinic treated mice but group B had a significant (p < 0.05) higher weight gain compared to the control. TEC and PCV increased significantly (p < 0.05) in all the treatment groups compared to the ...

Bangladesh Journal of Veterinary Medicine, 2008

In the present study, five Black Bengal goats (Capra hircus) were used to investigate the bones o... more In the present study, five Black Bengal goats (Capra hircus) were used to investigate the bones of fore limb during January to June 2006. It was observed topographically that the scapula was more or less similar to other ruminant animal topographically with exception that, the presence of very short coracoid process, more or less oval shaped glenoid cavity and more extensive subscapular fossa. The humerus of adult Black Bengal goat was 12.06 ± 0.27cm in length. The diameter of shaft at the level of nutrient foramen was 4.24 ± 0.05 cm. The breadth of humerus was 1.66 ± 0.06 cm. The deltoid tuberosity was less prominent and there was shallow radial and olecranon fossa. The radius of adult Black Bengal goat was 11.12 ± 0.23 cm in length. The diameter of shaft of radius-ulna just below the interosseous space was 3.86 ± 0.12cm. The total length of ulna of adult Black Bengal goat was 14.20 ± 0.20 cm. The interosseous space was found very narrow in the present study. Six carpal bones...

The Journal of Poultry Science, 2008

The aims of this study was to find out the histological di erences of the mucosa and comparative ... more The aims of this study was to find out the histological di erences of the mucosa and comparative analysis of Ig-containing plasma cells among the di erent segments of the gastrointestinal tract of broiler and native chickens of Bangladesh. The conventional histological study revealed that the lining epithelium of the proximal segments (esophagus, crop, and proventriculus) were thicker in the broiler. The esophageal glands were more in the broiler than the native chickens. The villi of the duodenum, jejunum and ileum were slender and longer in the broiler in comparison to the native chickens. The number of goblet cells in the duodenum, jejunum, and ileum was more in the native chickens than the broiler. The indirect immunohistochemistry revealed that very few immunoglobulin (Ig)-containing plasma cells were present in the epithelium and lamina propria of esophagus, crop, and proventriculus of the broiler and native chickens. The frequency of the population of these cells were abundantly located in the lamina propria, around the intestinal gland and in the core of the villi from duodenum to ileum of broiler and native chickens. The intraepithelial IgA-containing epithelium was observed only in the epithelium of the native chickens. Segmental variation of Ig-containing plasma cells was noticed in these two strains of chickens. The IgA-, IgG-, and IgM-containing plasma cells were significantly more in the most of the segments of the small intestine of the native chickens. This suggested that besides the existence of histological variation in the gastrointestinal tract of broiler and native chickens, the Ig-containing plasma cells were significantly more in the di erent segments of the digestive tract of native chickens possible due to their scavenging. : broiler, gastrointestinal tract, immunoglobulin cells, mucosa, native chickens (MALT) supply lymphocytes and plasma cells that con-cells, macrophages, dendritic cells and non-professional tribute to the local immune system (McDermott and antigen presenting cells (APCs) (McDonald and Spencer,). These cells principally developed and mobilize from "Gut Associated Lymphoid Tissue (GALT)", which may include esophageal tonsils, Meckel's diverticulum, Peyer's patches, cecal tonsils, and bursa of Fabricius (Arai). The plasma cells were the cells of the immune system that secrete large amounts of antibodies

Bangladesh Journal of Veterinary Medicine, 2014

The present research was designed to study the histological and immunohistochemical changes of ly... more The present research was designed to study the histological and immunohistochemical changes of lymphoid organs (bursa of Fabricius and cecal tonsil) and in the ileum (representative mucosal organ) of broiler chickens after immunization with Gumboro vaccine. Two groups (n=24) of Cobb-500 broiler chickens were reared in a same environment A mild vaccine (BUR® 706) followed by a intermediate (Nobilis® Gumboro 228 E) type vaccine was administered for immunization of chickens and samples were collected at 7 days interval for up to 32 days of age. The conventional histological structure of all three organs of the present study was similar to the earlier reports. In addition, in the bursa of Fabricius, the population of lymphocytes and the size of the follicles were found to increase in vaccinated chickens than the control chickens. The Igs positive cells (IgA, IgG and IgM) were distributed principally beneath the capsule, around the follicles and in the cortex and medulla. The frequencies...

International Journal of Veterinary Science and Medicine, 2013

The first appearance, distribution and frequency of immunoglobulins (Igs)-positive lymphocytes we... more The first appearance, distribution and frequency of immunoglobulins (Igs)-positive lymphocytes were investigated in the lymphoid organs of native chicken's embryos from embryonic day (ED) 8 to ED 20. The tissues from the lymphoid organs were dehydrated in alcohol, cleared in xylene, embedded in different grades of paraffin and 6-micron thick sections were immunostained by the indirect immunoperoxidase method using antichicken immunoglobulins. IgM-positive lymphocytes were first identified in the follicles of bursa of Fabricius at ED 10, in the white pulp of the spleen at ED 14 and in the lamina propria of the cecal tonsil at ED 20. Their frequencies of populations were statistically significant from ED 14 to ED 20. IgG-positive lymphocytes were first appeared in the bursa of Fabricius and spleen at ED 20. In the bursa IgG-positive lymphocytes were located in the medulla and cortical part of the follicles, whereas, in the spleen these immune cells were located around the white pulp. IgA-positive lymphocytes were not observed in any of the developing lymphoid organs of the present study. When the data for bursa of Fabricius, spleen,

Pakistan Veterinary …, 2011

Native chickens (Gallus domesticus) of Bangladesh are scavenging in nature. Data regarding morpho... more Native chickens (Gallus domesticus) of Bangladesh are scavenging in nature. Data regarding morphology of lymphoid organs in prenatal stages are lacking. H&E staining method was performed to study the development of the bursa of Fabricius, thymus, spleen and cecal tonsil from embryonic day (ED) 10 to 20. The budding of thymus was seen on ED 10. At ED 12, fiber network of thymus was formed to create a basement of cells of thymus and on ED 14 these cells began to organize to form cortex and medulla. But the cortex and medulla of thymus could not be differentiated before ED 20. The plicae of bursa Fabricius started to develop on ED 10. From ED 12 the plicae became shorter and wider to form bursal follicles and these follicles were clearly organized into cortex and medulla on ED 20. At ED 10, very thin capsule was seen in embryonic spleen. During ED 12, only a few white pulps were observed, while on ED 14, purple colored white pulp and pinkish red pulp were easily visible. At ED 20, the thickness of capsule was increased and pulps were more distinguishable. All the lymphoid organs showed major development during the later incubation period, indicating that the immune system in that period is being prepared to face the scavenging environment after hatching.