Jaime Zuloaga - Academia.edu (original) (raw)

Papers by Jaime Zuloaga

Clinical Infectious Diseases, 2005

Revista Española de Enfermedades Digestivas

Parasitology research, Jan 13, 2018

Anisakiasis is a global disease caused by consumption of raw or lightly cooked fish parasitised w... more Anisakiasis is a global disease caused by consumption of raw or lightly cooked fish parasitised with Anisakis spp. third-stage larvae. Cases in the literature show colocalised anisakiasis and colorectal cancer, and the incidental finding of Anisakis larvae at the tumour site was reported. Data from our group suggested an epidemiological link between previous infection and gastrointestinal cancer. Furthermore, it has recently been reported that Anisakis products lead to inflammation and DNA damage. Based on these facts, the aim was to investigate whether Anisakis antigens are able to induce changes in the proliferation of epithelial cells in vitro or in the expression of serum microRNA (miRNA) in Sprague-Dawley rats. Anisakis complete extract (CE) induced increases in cell proliferation and decreases in apoptosis compared with nontreated cells, which resulted in a significant increase in the absolute number of viable cells at 48 h of exposure (P < .05). Furthermore, the miRNAs mmu...

Annals of Pathology and Laboratory Medicine

Mucinous cystadenoma is a generic denomination usually applied to cystic formations filled with m... more Mucinous cystadenoma is a generic denomination usually applied to cystic formations filled with mucinous content and showing signs of epithelial proliferation. They usually arise in ovaries, pancreas and appendix, but there have been occasional reports in many other locations. Most retroperitoneal and mesenteric mucinous cystadenomas have affected women, and a potential müllerian origin has been postulated, although for most of them their histogenesis remains uncertain. Some cases have shown malignant behavior. We herein describe a case of mucinous cystadenoma affecting a young man with an unusual prerectal location. An exhaustive study was performed as a basis for the discussion of possible origins for this tumor. This lesion showed premalignant proliferative changes, suggesting an early stage in a potential malignant transformation.

Medicine, 2015

Anisakiasis is a global disease caused by consumption of raw or lightly cooked fish contaminated ... more Anisakiasis is a global disease caused by consumption of raw or lightly cooked fish contaminated with L3 Anisakis spp. larvae. High rates of parasitization of fish worldwide make Anisakis a serious health hazard. In fact, anisakiasis is a growing disease in countries such as Spain, Italy, and Japan, where consumption of raw/marinated fish is high. Some parasitic infections have been recognized as a causative factor for human cancer. Suggested mechanisms include chronic inflammation elicited by the parasite, and a possible tumorigenic effect from certain parasitic secretions. Anisakis can produce persistent local inflammation and granuloma, and larvae have been incidentally found in gastrointestinal (GI) tumors. Our aim was to discover possible differences in the prevalence of unnoticed or asymptomatic previous Anisakis infection in GI cancer patients compared with healthy individuals. Serum levels of specific antibodies against Anisakis antigens were used as a reliable marker of previous contact with their larvae. Ninety-four participants without a previous history of Anisakis infection were prospectively allocated into 1 of 2 groups: 47 patients with GI cancer and 47 controls. Specific IgE, IgA1, and IgG1 against the Anisakis recombinant antigens Ani s 1, Ani s 5, Ani s 9, and Ani s 10 were determined by an ELISA assay. The ratio of positivity to sIgA1, rAni s 1, or rAni s 5 was significantly higher in the cancer patients than in the controls (38.30% vs 6.38%, P < 0.001) and (42.55% vs 10.64%, P < 0.001, respectively). When disaggregated by type of tumor, the patients with gastric cancer showed a higher proportion of positive results for sIgA1 to rAni s 1 (P < 0.001), whereas a higher proportion of colon cancer patients were shown to be positive for sIgA1 to both rAni s 1 (P < 0.05) and rAni s 5 (P < 0.01). Earlier Anisakis infection might be a risk factor for the development of stomach or colon cancer.

Revista Espanola De Enfermedades Digestivas, 2008

La colitis isquémica se complica con el desarrollo de estenosis en el 10-15% de los casos (1). El... more La colitis isquémica se complica con el desarrollo de estenosis en el 10-15% de los casos (1). El edema asociado con la isquemia y la fibrosis secundaria al proceso cicatricial se postulan como los mecanismos de dicha estenosis (2). Aunque son relativamente frecuentes las estenosis isquémicas de colon tras cirugía del aneurisma aórtico, existen muy pocos casos descritos tras resección rectal (3), estando casi todos ellos relacionados con radioterapia perioperatoria. Aquí describimos un caso de estenosis isquémica de colon, no relacionada con radioterapia, tras una resección anterior realizada 5 años antes, planteando un difícil diagnóstico diferencial con recidiva tumoral. CASO CLÍNICO Paciente varón de 65 años, fumador de 20-40 cigarrillos/día y con antecedente de ulcus duodenal. Había sido intervenido 5 años previamente por neoplasia estenosante de unión recto-sigmoidea (adenocarcinoma estadio B de Dukes, T3N0M0, estadio III-A AJCC), practicándosele resección anterior, seguida de anastomosis término-terminal mediante sutura mecánica utilizando la técnica del doble grapado. El postoperatorio de dicha intervención cursó sin complicaciones y el paciente fue seguido en consulta cada 6 meses, realizándose anualmente colonoscopia y ecografía. La imagen endoscópica de la región perianastomótica resultó normal en todas las revisiones, incluyendo la última, realizada el año anterior (a los 4 años de la intervención).

Revista Española de Enfermedades Digestivas, 2013

Parasitology Research, 2013

Anisakiasis is a fish-borne parasitic disease caused by consumption of raw or undercooked fish or... more Anisakiasis is a fish-borne parasitic disease caused by consumption of raw or undercooked fish or cephalopods parasited by Anisakis spp. third stage larvae. The pathological effects of the infection are the combined result of the mechanical action of the larva during tissue invasion, the direct tissue effects of the excretory/secretory products released by the parasite, and the complex interaction between the host immune system and the Anisakis antigens. The aim of this study was to develop an experimental model of infection with Anisakis spp. live larvae in rats, useful to study the acute and chronic histopathological effects of the Anisakis infection. Sprague-Dawley rats were subjected to esophageal catheterization to place larvae directly into the stomach. Reinfections at different intervals after the first infection were preformed. Live larvae were found anchored to the mucosa and passing through the wall of the stomach and showed a strong resistance being able to stay alive at different sites and at the different pH. Migration of larvae from the stomach to other organs out of the

Digestive and Liver Disease, 2006

The growing popularity in western countries of eating uncooked seafood has resulted in an increas... more The growing popularity in western countries of eating uncooked seafood has resulted in an increased incidence of anisakidosis. To study the in vitro activity of different concentrations of albendazole against Anisakis simplex larvae under different media pH. A. simplex larvae were obtained from fresh hakes acquired from the fish market of Madrid. They were divided into groups and placed in culture dishes (15 larvae each) containing RPMI-1640, in the presence or absence of different concentrations of albendazole (300, 400 and 500 microg/mL). Albendazole dose-dependently reduced the survival of the larvae, its maximum activity being at 500 microg/mL when it killed almost all larvae at 48 h. Acidic medium pH significantly reduced the efficacy of albendazole. Albendazole is effective in killing A. simplex larvae at different pH in vitro, suggesting that this molecule could be useful in treating clinical manifestations of human anisakidosis.

Blood, 2009

Chronic lymphocytic leukemia (CLL) cell migration into lymphoid tissues is an important aspect of... more Chronic lymphocytic leukemia (CLL) cell migration into lymphoid tissues is an important aspect of the pathobiology of this disease. Here, we investigated the role of ephrin-A4 (EFNA4) in the transendothelial migration (TEM) capacity of CLL and normal B cells through interacting with endothelial EphA2 (erythropoietin-producing hepatocellular carcinoma). CLL cells showed a remarkable impairment in the adhesion to and transmigration through human umbilical vein endothelial cell (HUVEC) monolayers, correlating with their higher EFNA4 expression. In vitro, TEM was mediated by EFNA4 binding to endothelial EphA2 receptor, which is highly expressed in tumor necrosis factor-α–activated HUVECs as well as in the CD31+ endothelial cells of human lymph nodes. The pretreatment of CLL cells with EphA2 homodimers further impaired their adhesion to and transmigration through HUVEC monolayers, whereas pretreatment of HUVECs with EFNA4 homodimers improved those phenomena in both CLL and normal B cells...

Cirugia Espanola, 2004

... Jaime Zuloaga, Javier Arias y José L. Balibrea Departamento de Cirugía. ... Correspondencia: ... more ... Jaime Zuloaga, Javier Arias y José L. Balibrea Departamento de Cirugía. ... Correspondencia: Dr.Jaime Zuloaga Bueno. ... Bibliografía 1. López-Peñas D, Raírez-Ortiz LM, Del Rosal-Palomeque R, López-Rubio F, Fernández-Crehuet R, Miño-Fugarolas G. Anisakiasis en España ...