Journal of Regenerative Biology and Medicine (original) (raw)
Volume 1 Issue 1 by Journal of Regenerative Biology and Medicine
Journal of Regenerative Biology and Medicine, Jun 21, 2019
Stem Cell Science/Regenerative Medicine is perhaps the most important “pillar” out of the four pi... more Stem Cell Science/Regenerative Medicine is perhaps the most important “pillar” out of the four pillars of modern medicine. Use and abuse of the “Stem Cells and Stem Cell derived Products” as therapeutic measure for treatment of several untreatable diseases has come to the fore in recent times. The “stem cells” are drugs as discussed below. In the realm of clinical trials, to be approved by FDA, just as a “drug” undergoes trials, successively via phase I, phase II and phase III trials, likewise stem cells, which are “drugs” per se has to be channelled through the same three phases of clinical trials.
There are some evolving regulations in place for regulating stem cell research and its prospective human application in clinical trials which is in place, as per the guidelines of ICSSR 2016. Each and every country, including India has more or less similar rules and regulations which conform to the rules/regulations as proposed by ICSSR and followed by FDA, although many countries has done individual modifications in the same.
Stem Cell Science/Regenerative Medicine is perhaps the most important “pillar” out of the four pillars of modern medicine. Use and abuse of the “Stem Cells and Stem Cell derived Products” as therapeutic measure for treatment of several untreatable diseases has come to the fore in recent times. The “stem cells” are drugs as discussed below. In the realm of clinical trials, to be approved by FDA, just as a “drug” undergoes trials, successively via phase I, phase II and phase III trials, likewise stem cells, which are “drugs” per se has to be channelled through the same three phases of clinical trials. There are some evolving regulations in place for regulating stem cell research and its prospective human application in clinical trials which is in place, as per the guidelines of ICSSR 2016. Each and every country, including India has more or less similar rules and regulations which conform to the rules/regulations as proposed by ICSSR and followed by FDA, although many countries has done individual modifications in the same. The role of NAC-SCRT, ICMR, IEC, IC-SCR, DCGI are clearly defined and it has been clearly stated in NGSCR that approval for stem cell application in patients, (Autologous, allogenic, homologous or non-homologous) only within the confines of “clinical trials” and therapy based on Stem Cells and Stem Cell derived Products (SCDP) should be informed in a proper format to the regulatory concern and done through the proper channel.
All laboratories and institutes coming under the umbrella of NGSCR should have GMP, GLP and GCP compliant. Appropriate clean room classification shall be followed for stem cell isolation, culturing or manipulation in their respective lab/institutes. The safety and efficacy concerns regarding animal and human applications, uniformity in batch production, strengthening of scientific knowhow should definitely be in line with the cutting-edge technological advancements globally. Conformity with international guidelines, and peer reviewed publications are a must for getting approval for clinical trials and marketing options. The review as a whole may not be comprehensive enough to include all the points in relation to the regulatory domain for approving SCDP in our country but an attempt has been made to identify the important points keeping in mind that this domain will evolve and rules and regulations will change with the passage of time.
Journal of Regenerative Biology and Medicine, Aug 19, 2019
This paper will discuss what the Human Papillomavirus (HPV) is. Ethical questions posed are “Shou... more This paper will discuss what the Human Papillomavirus (HPV) is. Ethical questions posed are “Should the HPV (Human Papillomavirus) vaccine be required? If yes at what age and should it be required for both boys and girls? During the paper another ethical question that will be discussed is, “On what basis should parents be permitted to refuse and be exempt from the mandate”? An analysis of the Human papillomavirus will explain what is HPV, how it is passed between people, any symptoms that could occur, and information about treatment, vaccines, and prevention. The role and responsibilities of the Family Nurse Practitioner, clinical aspects, scientific evidence, conflicts, social issues, contextual facts and stakeholders will all be discussed. In addition, bioethical principles autonomy, informed consent, beneficence, non-malfeasance, and justice will be discussed that are relevant and applicable to the ethical issues associated with the Human papillomavirus and HPV vaccine.
Journal of Regenerative Biology and Medicine, Aug 30, 2019
Saudi and Egyptian pomegranate juice (APJ and EPJ) contains potent polyphenols, antioxidants that... more Saudi and Egyptian pomegranate juice (APJ and EPJ) contains potent polyphenols, antioxidants that were found to reduce serum and macrophage oxidative stress. The protective effects of APJ and EPJ against atherogenicity were evaluated by feeding mice a hypercholesterolemic diet that was supplemented with PJ.PJ consumption reduced mice serum triglycerides (TG), total cholesterol (TC) and (low density lipoprotein (LDL-c) and increased the high-density lipoprotein (HDL-c) when compared to mice with a control or atherogenic diet. The risk ratio and atherogenic index were significantly lower in both APJ or EPJ supplemented groups. Paraoxonase 1 (PON1), which remains associated with HDL, had a significant increase in activity in mice supplemented with PJ, compared to mice with aatherogenic diet (AD). PJ consumption significantly reduced lipid peroxidation and increased glutathione levels. The reduction in lipid peroxidation varied from 57 to 76%. PJ supplementation reduced peritoneal macrophage (MPM)-mediated oxidation of LDL by 68 to 82% and decreased mouse MPM Ox-LDL uptake by 31 to 48%. A significant up-regulation of macrophage PON2 activity was induced by PJ reducing the macrophage oxidative stress. In conclusion, PJ consumption resulted in anti-oxidative and anti-atherogenic effect on serum and macrophages, which could prevent atherosclerosis and coronary heart diseases.
Journal of Regenerative Biology and Medicine, Sep 30, 2019
In this study 60 milk samples collected from mastitic cows to isolates and identify bacterial age... more In this study 60 milk samples collected from mastitic cows to isolates and identify bacterial agents. The percentage of isolates was as follows: Staphylococcus spp 52%, Bacillus spp 26%, Enteroccocispp 3%; microccus 3%, Nesseria 2%, Branhamella 3%, Clostridium 1%, Bordetella 2%, Enterobacteria 3%, Aeromonas 4%, Fusobacterium 1%. In sensitivity tests used two antibiotics Amoxicillin and Cephalexin against two highest isolates in this study (Staph spp and Bacillus spp and found: Staph. aureus and Staph. hyicus were resistant to Amoxicllin and cephlaxin relatively, Bacillus coagulans and Bacillus cereus were susceptible to Amoxicllin and cephlaxin.
Journal of Regenerative Biology and Medicine, Sep 24, 2019
Background: Junk foods are rich in calories, salt and fats. Excess consumption of junk foods woul... more Background: Junk foods are rich in calories, salt and fats. Excess consumption of junk foods would lead rise to wide variety of health disorders. Objective: the purpose of this study is to find out about the fast-food consumption of adolescent in age between 13-25 years old in Derna city and their effect on health. Methods: the sample of this research included 100 adolescents from both sexes, a descriptive crosssectional study of teenagers was conducted. The questionnaire was divided into two parts: first part related to socio-demographic information and part two related to junk food pattern and influencing factors of junk food consumption and their effect on health. Data were analyzed using SPSS version 24. A value of P < 0.05 was interpreted as statistically significant.
Results: The findings revealed that more girls (67.0%) consumed fast food than boys and approximately half (49.0%) of Participants were consumed fast food as an alternative to main meal and more than half of participants (63.0%) were consumed soft drink every day, furthermore greater proportion of participants (42.0%) had urinary tract infection and that may relate to chips and soft drink were the most attractive food items among Participants.
Conclusion: adolescent consumed a greater amount of junk food which led to a majority of ill effects later on. It is recommended that the schools and community conduct and implement awareness programmers on fast food consumption and its ill effects.
Journal of Regenerative Biology and Medicine, Oct 1, 2019
Diarthrodial joints, such as the knee, hip, and shoulder consist of articular cartilage, a synovi... more Diarthrodial joints, such as the knee, hip, and shoulder consist of articular cartilage, a synovial capsule, and a fibrocartilaginous structure to increase the stability of the joint. The knee has both a medial and lateral meniscus. The patient’s shoulder and hip each have a labrum which increases the concavity of the glenoid and acetabulum. These fibrocartilaginous structures all have a nerve supply and when torn, can be very painful. These structures have a limited blood supply and when damaged, have minimal capacity to heal. These structures can tear with an acute injury or by a chronic degenerative process. Bone marrow-derived MSC therapy is standard of care in veterinary medicine. There is increasing interest in using biologics to potentially heal fibrocartilaginous injuries in the shoulder, knee, and hip to avoid surgery. The future acellular treatment for labral tears will involve a two-step process: first, highly concentrated anti-inflammatory bone marrow-derived MSC growth factors and exosomes are injected into the painful joint. Second, these growth factors and exosomes enter the recipient synovial MSCs to stimulate the production of new chondroblasts and anti-inflammatory secretomes, chemokines, and cytokines. These effects have been shown to heal fibrocartilaginous tears. This acellular biologic treatment can all be achieved with a single joint injection. The future of regenerative medicine in orthopedics and spine may well be the utilization of highly concentrated acellular MSC derived growth factors and especially exosomes.
Volume 1 Issue 2 by Journal of Regenerative Biology and Medicine
Journal of Regenerative Biology and Medicine, Dec 20, 2019
To fix and prevent, not ‘manage’ Osteoarthritis OA in the lower limb, should be the collective ‘H... more To fix and prevent, not ‘manage’ Osteoarthritis OA in the lower limb, should be the collective ‘Holy Grail’ with or without Orthobiologics OB. Lateral Ankle Sprain LAS, Chronic Ankle Instability CAI and Ankle Osteoarthritis AOA create asymmetry which alters the biomechanics of the entire lower limb, so by better addressing AOA, we can probably do more than just impact the multi-billion dollar annual costs of AOA.
It would seem we have advanced, if not futuristic Surgical techniques and Orthobiologic technology, so what is missing? The short answer is Medical Intent MI. Devices and methods used in rehabilitation need MI intent to enable and stimulate repair. The world is changing and Morals Ethics and the human costs, are being counted. CTE concussion is just the tip of the iceberg for cumulative trauma injuries, in cost and prevalence, and class actions seek to defend and enforce people’s rights to safety, either in the workplace, as in professional sport, or in medical outcomes.
The significant yet hidden role of the subtalar joint as a ‘Safety Valve’ was first noted by Albert Ferguson in 1972, yet today the contradictions in rehabilitation and injury prevention devices that restrict the STJ, remain commonplace. It is also necessary to consider what has changed inside the synovial capsule before and after lateral ligament injury, such as pressure, joint alignment and space, so we can better understand and design for restoration of homeostasis.
This paper will examine factors, causes and interventions that may be inadvertently restricting or preventing Orthobiologics effectiveness in the human ankle from a Translational Medicine perspective and an engineering bias.
Journal of Regenerative Biology and Medicine, Dec 15, 2019
Stem cell therapies are continuing to be researched and implemented to treat a wide range of diso... more Stem cell therapies are continuing to be researched and implemented to treat a wide range of disorders that result in blindness. Major structures of the eye most prevalently tied to blindness are the cornea, lens, and retina, and specific conditions resulting in blindness includes corneal disease, stromal scarring, cataracts, age-related macular degeneration, retinitis pigmentosa, glaucoma, and diabetic retinopathy. This review covers a detailed description of the structures of the eye, the causes and corresponding disease pathways of each form of corneal, lens, and retinal dysfunction that results in blindness, and the current stem cell sources being researched and implemented, through animal models and clinical trials, to treat blindness. The review evaluates the progress of stem cell research and trials and presents the obstacles that remain to be overcome before stem cell sources to treat blindness can become standard clinical practice.
Journal of Regenerative Biology and Medicine, Oct 21, 2019
There is increasing published literature to support the safety and efficacy of IV infusions of bo... more There is increasing published literature to support the safety and efficacy of IV infusions of bone marrow-derived expanded allogeneic mesenchymal stem cells (MSCs) for the treatment of various auto-immune diseases. Frailty Syndrome was created to provide a way of objectively measuring aging with physical activity scales and bio-inflammatory markers. IV infusions of allogeneic MSCs have been reported to statistically significantly increase physical function and decrease inflammatory biomarkers in Frailty Syndrome. Replacing cellular allogeneic IV infusions with acellular bone marrow-derived MSC extracellular vesicle isolate products (EVIP) containing active growth factors (GFs) and exosomes has numerous advantages. Regenerative medicine researchers and clinicians now realize that living MSCs are not required to achieve clinical efficacy.The clinical efficacy of MSCs is due to their paracrine release of GFs and exosomes. Living MSCs are not required to accomplish the paracrinesignaling of GFs and exosomes. Acellular MSC EVIP containing active GFs and exosomes are the future of regenerative medicine. Acellular exosomesderived from bone marrow MSCs provide a consistent product that has extensive characterization, which includes advanced particle analysis, proteomic evaluation and USP sterility assurance. The future “Fountain of Youth” will be the frequent (3 to 4 times per year) IV infusion of bio pharmacologic quality bone marrow-derived MSC EVIP. These active GF and exosome infusions will result in a continual down regulation of systemic inflammation and based on published research reverse many of the inflammatory effects of aging.
Journal of Regenerative Biology and Medicine, Oct 14, 2019
The pathogenesis of steatohepatitis, which develops on the background of non-alcoholic fatty live... more The pathogenesis of steatohepatitis, which develops on the background of non-alcoholic fatty liver disease and may be the cause of liver cirrhosis, is considered in the article. Also, the paper focuses on the prospects of using peptide drugs for the treatment of liver cirrhosis. The study found that the preparation of plant regulatory peptides GA-40 has pronounced hepatoprotective, anti inflammatory and anti-fibrotic effects, which is confirmed by the elastometry data of the shear wave. The results of this work indicate that the use of the drug GA-40 was accompanied not only by the normalization of the inflammatory panel of the liver and triglycerides, but also by a likely decrease in the level of glypican-3 - the main factor that causes the development of primary liver cancer. Gene protector and immunocorrector GA-40, harmonizing metabolic processes in the liver, as well as suppressing inflammation and preventing excessive output of hepatocytes in apoptosis, has a positive effect on the prognosis of the disease. Reduced expression of glypican-3 in patients in the study group indicates that GA-40 is able to control the level of pro-oncogenic trigger proteins, thus reducing the risk of primary liver cancer.
Journal of Regenerative Biology and Medicine, Oct 1, 2019
The future of medicine is in biologics and artificial intelligence AI. With the advent of advanci... more The future of medicine is in biologics and artificial intelligence AI. With the advent of advancing and disruptive technologies, many traditional therapies are being replaced by new therapies such as: stem cell therapy, joint preservation, limb saving technologies and medical robotics. In countries where first world medicine is available, there will emerge new challenges in retraining for practitioners and confusion for patients as to what are the best treatment options.
From the medical practitioners’ perspective, the challenge will be to stay up to date with the changing times and innovations. Some aspects of their practice may quickly become obsolete. As patients become more aware of symptoms of their conditions, self-diagnosis will be on the rise, and patients may often run the risk of misdiagnosing themselves, or experiencing conditions such as cyberphobia from information overload. Innovations such as Artificial Intelligence will emerge, to summarize the vast amount of information that is being published each day relevant to medical fields. Artificial Intelligence that will interact with the doctor, promoting plausible diagnoses and treatment options is already present. Embracing change will become the norm.
Journal of Regenerative Biology and Medicine, Oct 1, 2019
The scientific study of medicine is all about the science of healing. Whether the focus be on the... more The scientific study of medicine is all about the science of healing. Whether the focus be on the mind or body, the fascination with prolonging life, improving the quality of life, and generating new life is a theme that will perpetuate the human experience. Organisms are self-contained growth and healing mechanisms. Science has marveled for years on how these healing processes work, so that they can be engineered into the right circumstances to restore anything from joint cartilage, ligaments, blood vessels, muscles and bones.
Papers by Journal of Regenerative Biology and Medicine
Journal of Regenerative Biology and Medicine, Jan 17, 2020
Cadaver and radiographic studies have demonstrated shoulder osteoarthritis (OA) in up to 33% of p... more Cadaver and radiographic studies have demonstrated shoulder osteoarthritis (OA) in up to 33% of patients over the age of 60. Patients that present with shoulder OA have pain, crepitus and loss of glenohumeral motion. Limiting the ability to place your hand where you desire severely impairs activities of daily living. The nonsurgical treatments for shoulder OA include shoulder exercises, the use of analgesics and non-steroidal anti-inflammatory medications. If these non-surgical treatments fail to relieve the OA impairment, the surgical treatment is total shoulder arthroplasty (TSA).
Journal of Regenerative Biology and Medicine, Jan 1, 2020
Osteoarthritis has eluded a curative/disease modifying treatment despite extensive research over ... more Osteoarthritis has eluded a curative/disease modifying treatment despite extensive research over the last century. This is largely due to the extremely slow metabolic turnover of articular cartilage in an essentially avascular environment, along with a pro-catabolic inflammatory cascade that is induced by damage to the healthy cartilage structure. There has been promising data emerging whereby this poor chondrocyte healing process can be improved by applying autologous stem cell populations (harvested from marrow/adipose tissue) that have been programmed to undergo rapid and sustained chondrogenesis with the assistance of numerous chondrogenic growth factors. Here we aim to provide a comprehensive review article about the growth factors employed for the purpose of articular cartilage rejuvenation. Disease modifying agents incorporating chondrogenic growth factors have been extensively researched in the last 2 decades, and it has been identified that the likely chondrogenic growth factor families of most therapeutic value are the Transforming Growth Factor beta (TGF-B superfamily), Fibroblastic Growth Factor (FGF - specifically FGF-18) and Insulin Growth Factor (IGF) in combination with many of the aforementioned factors. There is still a need for consensus on appropriate dosing and long-term studies should be performed to assess the durability of current therapies over many years. The application of growth factor enriched stem cell populations to osteoarthritic cartilage appears to be very near to effective therapeutic use.
Journal of Regenerative Biology and Medicine, Jun 21, 2019
Stem Cell Science/Regenerative Medicine is perhaps the most important “pillar” out of the four pi... more Stem Cell Science/Regenerative Medicine is perhaps the most important “pillar” out of the four pillars of modern medicine. Use and abuse of the “Stem Cells and Stem Cell derived Products” as therapeutic measure for treatment of several untreatable diseases has come to the fore in recent times. The “stem cells” are drugs as discussed below. In the realm of clinical trials, to be approved by FDA, just as a “drug” undergoes trials, successively via phase I, phase II and phase III trials, likewise stem cells, which are “drugs” per se has to be channelled through the same three phases of clinical trials.
There are some evolving regulations in place for regulating stem cell research and its prospective human application in clinical trials which is in place, as per the guidelines of ICSSR 2016. Each and every country, including India has more or less similar rules and regulations which conform to the rules/regulations as proposed by ICSSR and followed by FDA, although many countries has done individual modifications in the same.
Stem Cell Science/Regenerative Medicine is perhaps the most important “pillar” out of the four pillars of modern medicine. Use and abuse of the “Stem Cells and Stem Cell derived Products” as therapeutic measure for treatment of several untreatable diseases has come to the fore in recent times. The “stem cells” are drugs as discussed below. In the realm of clinical trials, to be approved by FDA, just as a “drug” undergoes trials, successively via phase I, phase II and phase III trials, likewise stem cells, which are “drugs” per se has to be channelled through the same three phases of clinical trials. There are some evolving regulations in place for regulating stem cell research and its prospective human application in clinical trials which is in place, as per the guidelines of ICSSR 2016. Each and every country, including India has more or less similar rules and regulations which conform to the rules/regulations as proposed by ICSSR and followed by FDA, although many countries has done individual modifications in the same. The role of NAC-SCRT, ICMR, IEC, IC-SCR, DCGI are clearly defined and it has been clearly stated in NGSCR that approval for stem cell application in patients, (Autologous, allogenic, homologous or non-homologous) only within the confines of “clinical trials” and therapy based on Stem Cells and Stem Cell derived Products (SCDP) should be informed in a proper format to the regulatory concern and done through the proper channel.
All laboratories and institutes coming under the umbrella of NGSCR should have GMP, GLP and GCP compliant. Appropriate clean room classification shall be followed for stem cell isolation, culturing or manipulation in their respective lab/institutes. The safety and efficacy concerns regarding animal and human applications, uniformity in batch production, strengthening of scientific knowhow should definitely be in line with the cutting-edge technological advancements globally. Conformity with international guidelines, and peer reviewed publications are a must for getting approval for clinical trials and marketing options. The review as a whole may not be comprehensive enough to include all the points in relation to the regulatory domain for approving SCDP in our country but an attempt has been made to identify the important points keeping in mind that this domain will evolve and rules and regulations will change with the passage of time.
Journal of Regenerative Biology and Medicine, Aug 19, 2019
This paper will discuss what the Human Papillomavirus (HPV) is. Ethical questions posed are “Shou... more This paper will discuss what the Human Papillomavirus (HPV) is. Ethical questions posed are “Should the HPV (Human Papillomavirus) vaccine be required? If yes at what age and should it be required for both boys and girls? During the paper another ethical question that will be discussed is, “On what basis should parents be permitted to refuse and be exempt from the mandate”? An analysis of the Human papillomavirus will explain what is HPV, how it is passed between people, any symptoms that could occur, and information about treatment, vaccines, and prevention. The role and responsibilities of the Family Nurse Practitioner, clinical aspects, scientific evidence, conflicts, social issues, contextual facts and stakeholders will all be discussed. In addition, bioethical principles autonomy, informed consent, beneficence, non-malfeasance, and justice will be discussed that are relevant and applicable to the ethical issues associated with the Human papillomavirus and HPV vaccine.
Journal of Regenerative Biology and Medicine, Aug 30, 2019
Saudi and Egyptian pomegranate juice (APJ and EPJ) contains potent polyphenols, antioxidants that... more Saudi and Egyptian pomegranate juice (APJ and EPJ) contains potent polyphenols, antioxidants that were found to reduce serum and macrophage oxidative stress. The protective effects of APJ and EPJ against atherogenicity were evaluated by feeding mice a hypercholesterolemic diet that was supplemented with PJ.PJ consumption reduced mice serum triglycerides (TG), total cholesterol (TC) and (low density lipoprotein (LDL-c) and increased the high-density lipoprotein (HDL-c) when compared to mice with a control or atherogenic diet. The risk ratio and atherogenic index were significantly lower in both APJ or EPJ supplemented groups. Paraoxonase 1 (PON1), which remains associated with HDL, had a significant increase in activity in mice supplemented with PJ, compared to mice with aatherogenic diet (AD). PJ consumption significantly reduced lipid peroxidation and increased glutathione levels. The reduction in lipid peroxidation varied from 57 to 76%. PJ supplementation reduced peritoneal macrophage (MPM)-mediated oxidation of LDL by 68 to 82% and decreased mouse MPM Ox-LDL uptake by 31 to 48%. A significant up-regulation of macrophage PON2 activity was induced by PJ reducing the macrophage oxidative stress. In conclusion, PJ consumption resulted in anti-oxidative and anti-atherogenic effect on serum and macrophages, which could prevent atherosclerosis and coronary heart diseases.
Journal of Regenerative Biology and Medicine, Sep 30, 2019
In this study 60 milk samples collected from mastitic cows to isolates and identify bacterial age... more In this study 60 milk samples collected from mastitic cows to isolates and identify bacterial agents. The percentage of isolates was as follows: Staphylococcus spp 52%, Bacillus spp 26%, Enteroccocispp 3%; microccus 3%, Nesseria 2%, Branhamella 3%, Clostridium 1%, Bordetella 2%, Enterobacteria 3%, Aeromonas 4%, Fusobacterium 1%. In sensitivity tests used two antibiotics Amoxicillin and Cephalexin against two highest isolates in this study (Staph spp and Bacillus spp and found: Staph. aureus and Staph. hyicus were resistant to Amoxicllin and cephlaxin relatively, Bacillus coagulans and Bacillus cereus were susceptible to Amoxicllin and cephlaxin.
Journal of Regenerative Biology and Medicine, Sep 24, 2019
Background: Junk foods are rich in calories, salt and fats. Excess consumption of junk foods woul... more Background: Junk foods are rich in calories, salt and fats. Excess consumption of junk foods would lead rise to wide variety of health disorders. Objective: the purpose of this study is to find out about the fast-food consumption of adolescent in age between 13-25 years old in Derna city and their effect on health. Methods: the sample of this research included 100 adolescents from both sexes, a descriptive crosssectional study of teenagers was conducted. The questionnaire was divided into two parts: first part related to socio-demographic information and part two related to junk food pattern and influencing factors of junk food consumption and their effect on health. Data were analyzed using SPSS version 24. A value of P < 0.05 was interpreted as statistically significant.
Results: The findings revealed that more girls (67.0%) consumed fast food than boys and approximately half (49.0%) of Participants were consumed fast food as an alternative to main meal and more than half of participants (63.0%) were consumed soft drink every day, furthermore greater proportion of participants (42.0%) had urinary tract infection and that may relate to chips and soft drink were the most attractive food items among Participants.
Conclusion: adolescent consumed a greater amount of junk food which led to a majority of ill effects later on. It is recommended that the schools and community conduct and implement awareness programmers on fast food consumption and its ill effects.
Journal of Regenerative Biology and Medicine, Oct 1, 2019
Diarthrodial joints, such as the knee, hip, and shoulder consist of articular cartilage, a synovi... more Diarthrodial joints, such as the knee, hip, and shoulder consist of articular cartilage, a synovial capsule, and a fibrocartilaginous structure to increase the stability of the joint. The knee has both a medial and lateral meniscus. The patient’s shoulder and hip each have a labrum which increases the concavity of the glenoid and acetabulum. These fibrocartilaginous structures all have a nerve supply and when torn, can be very painful. These structures have a limited blood supply and when damaged, have minimal capacity to heal. These structures can tear with an acute injury or by a chronic degenerative process. Bone marrow-derived MSC therapy is standard of care in veterinary medicine. There is increasing interest in using biologics to potentially heal fibrocartilaginous injuries in the shoulder, knee, and hip to avoid surgery. The future acellular treatment for labral tears will involve a two-step process: first, highly concentrated anti-inflammatory bone marrow-derived MSC growth factors and exosomes are injected into the painful joint. Second, these growth factors and exosomes enter the recipient synovial MSCs to stimulate the production of new chondroblasts and anti-inflammatory secretomes, chemokines, and cytokines. These effects have been shown to heal fibrocartilaginous tears. This acellular biologic treatment can all be achieved with a single joint injection. The future of regenerative medicine in orthopedics and spine may well be the utilization of highly concentrated acellular MSC derived growth factors and especially exosomes.
Journal of Regenerative Biology and Medicine, Dec 20, 2019
To fix and prevent, not ‘manage’ Osteoarthritis OA in the lower limb, should be the collective ‘H... more To fix and prevent, not ‘manage’ Osteoarthritis OA in the lower limb, should be the collective ‘Holy Grail’ with or without Orthobiologics OB. Lateral Ankle Sprain LAS, Chronic Ankle Instability CAI and Ankle Osteoarthritis AOA create asymmetry which alters the biomechanics of the entire lower limb, so by better addressing AOA, we can probably do more than just impact the multi-billion dollar annual costs of AOA.
It would seem we have advanced, if not futuristic Surgical techniques and Orthobiologic technology, so what is missing? The short answer is Medical Intent MI. Devices and methods used in rehabilitation need MI intent to enable and stimulate repair. The world is changing and Morals Ethics and the human costs, are being counted. CTE concussion is just the tip of the iceberg for cumulative trauma injuries, in cost and prevalence, and class actions seek to defend and enforce people’s rights to safety, either in the workplace, as in professional sport, or in medical outcomes.
The significant yet hidden role of the subtalar joint as a ‘Safety Valve’ was first noted by Albert Ferguson in 1972, yet today the contradictions in rehabilitation and injury prevention devices that restrict the STJ, remain commonplace. It is also necessary to consider what has changed inside the synovial capsule before and after lateral ligament injury, such as pressure, joint alignment and space, so we can better understand and design for restoration of homeostasis.
This paper will examine factors, causes and interventions that may be inadvertently restricting or preventing Orthobiologics effectiveness in the human ankle from a Translational Medicine perspective and an engineering bias.
Journal of Regenerative Biology and Medicine, Dec 15, 2019
Stem cell therapies are continuing to be researched and implemented to treat a wide range of diso... more Stem cell therapies are continuing to be researched and implemented to treat a wide range of disorders that result in blindness. Major structures of the eye most prevalently tied to blindness are the cornea, lens, and retina, and specific conditions resulting in blindness includes corneal disease, stromal scarring, cataracts, age-related macular degeneration, retinitis pigmentosa, glaucoma, and diabetic retinopathy. This review covers a detailed description of the structures of the eye, the causes and corresponding disease pathways of each form of corneal, lens, and retinal dysfunction that results in blindness, and the current stem cell sources being researched and implemented, through animal models and clinical trials, to treat blindness. The review evaluates the progress of stem cell research and trials and presents the obstacles that remain to be overcome before stem cell sources to treat blindness can become standard clinical practice.
Journal of Regenerative Biology and Medicine, Oct 21, 2019
There is increasing published literature to support the safety and efficacy of IV infusions of bo... more There is increasing published literature to support the safety and efficacy of IV infusions of bone marrow-derived expanded allogeneic mesenchymal stem cells (MSCs) for the treatment of various auto-immune diseases. Frailty Syndrome was created to provide a way of objectively measuring aging with physical activity scales and bio-inflammatory markers. IV infusions of allogeneic MSCs have been reported to statistically significantly increase physical function and decrease inflammatory biomarkers in Frailty Syndrome. Replacing cellular allogeneic IV infusions with acellular bone marrow-derived MSC extracellular vesicle isolate products (EVIP) containing active growth factors (GFs) and exosomes has numerous advantages. Regenerative medicine researchers and clinicians now realize that living MSCs are not required to achieve clinical efficacy.The clinical efficacy of MSCs is due to their paracrine release of GFs and exosomes. Living MSCs are not required to accomplish the paracrinesignaling of GFs and exosomes. Acellular MSC EVIP containing active GFs and exosomes are the future of regenerative medicine. Acellular exosomesderived from bone marrow MSCs provide a consistent product that has extensive characterization, which includes advanced particle analysis, proteomic evaluation and USP sterility assurance. The future “Fountain of Youth” will be the frequent (3 to 4 times per year) IV infusion of bio pharmacologic quality bone marrow-derived MSC EVIP. These active GF and exosome infusions will result in a continual down regulation of systemic inflammation and based on published research reverse many of the inflammatory effects of aging.
Journal of Regenerative Biology and Medicine, Oct 14, 2019
The pathogenesis of steatohepatitis, which develops on the background of non-alcoholic fatty live... more The pathogenesis of steatohepatitis, which develops on the background of non-alcoholic fatty liver disease and may be the cause of liver cirrhosis, is considered in the article. Also, the paper focuses on the prospects of using peptide drugs for the treatment of liver cirrhosis. The study found that the preparation of plant regulatory peptides GA-40 has pronounced hepatoprotective, anti inflammatory and anti-fibrotic effects, which is confirmed by the elastometry data of the shear wave. The results of this work indicate that the use of the drug GA-40 was accompanied not only by the normalization of the inflammatory panel of the liver and triglycerides, but also by a likely decrease in the level of glypican-3 - the main factor that causes the development of primary liver cancer. Gene protector and immunocorrector GA-40, harmonizing metabolic processes in the liver, as well as suppressing inflammation and preventing excessive output of hepatocytes in apoptosis, has a positive effect on the prognosis of the disease. Reduced expression of glypican-3 in patients in the study group indicates that GA-40 is able to control the level of pro-oncogenic trigger proteins, thus reducing the risk of primary liver cancer.
Journal of Regenerative Biology and Medicine, Oct 1, 2019
The future of medicine is in biologics and artificial intelligence AI. With the advent of advanci... more The future of medicine is in biologics and artificial intelligence AI. With the advent of advancing and disruptive technologies, many traditional therapies are being replaced by new therapies such as: stem cell therapy, joint preservation, limb saving technologies and medical robotics. In countries where first world medicine is available, there will emerge new challenges in retraining for practitioners and confusion for patients as to what are the best treatment options.
From the medical practitioners’ perspective, the challenge will be to stay up to date with the changing times and innovations. Some aspects of their practice may quickly become obsolete. As patients become more aware of symptoms of their conditions, self-diagnosis will be on the rise, and patients may often run the risk of misdiagnosing themselves, or experiencing conditions such as cyberphobia from information overload. Innovations such as Artificial Intelligence will emerge, to summarize the vast amount of information that is being published each day relevant to medical fields. Artificial Intelligence that will interact with the doctor, promoting plausible diagnoses and treatment options is already present. Embracing change will become the norm.
Journal of Regenerative Biology and Medicine, Oct 1, 2019
The scientific study of medicine is all about the science of healing. Whether the focus be on the... more The scientific study of medicine is all about the science of healing. Whether the focus be on the mind or body, the fascination with prolonging life, improving the quality of life, and generating new life is a theme that will perpetuate the human experience. Organisms are self-contained growth and healing mechanisms. Science has marveled for years on how these healing processes work, so that they can be engineered into the right circumstances to restore anything from joint cartilage, ligaments, blood vessels, muscles and bones.
Journal of Regenerative Biology and Medicine, Jan 17, 2020
Cadaver and radiographic studies have demonstrated shoulder osteoarthritis (OA) in up to 33% of p... more Cadaver and radiographic studies have demonstrated shoulder osteoarthritis (OA) in up to 33% of patients over the age of 60. Patients that present with shoulder OA have pain, crepitus and loss of glenohumeral motion. Limiting the ability to place your hand where you desire severely impairs activities of daily living. The nonsurgical treatments for shoulder OA include shoulder exercises, the use of analgesics and non-steroidal anti-inflammatory medications. If these non-surgical treatments fail to relieve the OA impairment, the surgical treatment is total shoulder arthroplasty (TSA).
Journal of Regenerative Biology and Medicine, Jan 1, 2020
Osteoarthritis has eluded a curative/disease modifying treatment despite extensive research over ... more Osteoarthritis has eluded a curative/disease modifying treatment despite extensive research over the last century. This is largely due to the extremely slow metabolic turnover of articular cartilage in an essentially avascular environment, along with a pro-catabolic inflammatory cascade that is induced by damage to the healthy cartilage structure. There has been promising data emerging whereby this poor chondrocyte healing process can be improved by applying autologous stem cell populations (harvested from marrow/adipose tissue) that have been programmed to undergo rapid and sustained chondrogenesis with the assistance of numerous chondrogenic growth factors. Here we aim to provide a comprehensive review article about the growth factors employed for the purpose of articular cartilage rejuvenation. Disease modifying agents incorporating chondrogenic growth factors have been extensively researched in the last 2 decades, and it has been identified that the likely chondrogenic growth factor families of most therapeutic value are the Transforming Growth Factor beta (TGF-B superfamily), Fibroblastic Growth Factor (FGF - specifically FGF-18) and Insulin Growth Factor (IGF) in combination with many of the aforementioned factors. There is still a need for consensus on appropriate dosing and long-term studies should be performed to assess the durability of current therapies over many years. The application of growth factor enriched stem cell populations to osteoarthritic cartilage appears to be very near to effective therapeutic use.