Jane Namukobe - Academia.edu (original) (raw)

Papers by Jane Namukobe

Alternative & Integrative Medicine, Oct 7, 2016

The East and Central African Journal of Pharmaceutical Sciences, Sep 29, 2021

High performance thin layer chromatography (HPTLC), high performance liquid chromatography (HPLC)... more High performance thin layer chromatography (HPTLC), high performance liquid chromatography (HPLC), column chromatography and Fourier transform infrared spectrophotometer (FTIR) were used for the identification and analysis of three phosphodiesterase type-5 (PDE-5) inhibitors in 50 randomly selected aphrodisiac herbal samples. Twenty-seven samples were found to contain one or two or all three synthetic PDE-5 inhibitors representing 54% of the analyzed samples. The FTIR spectral characteristics obtained from the isolated compounds were found to be in conformity with those of sildenafil, tadalafil and vardenafil reference standards. Patients who use these herbal sexual enhancers with the notion that they are safe and natural are likely to be exposed to serious health risks related to safety and quality of the herbal products. There is need for additional effort to effectively regulate herbal medicines in order to protect the consumers from the threat of adulteration.

BMC Complementary Medicine and Therapies

Background Spermacoce princeae (K. Schum) has been used in the treatment of bacterial skin infect... more Background Spermacoce princeae (K. Schum) has been used in the treatment of bacterial skin infections in Uganda. Pharmacological studies revealed that extracts of S. princeae exhibited antibacterial, antioxidant, and sun protection potential. This study aimed at isolating and identifying pure compounds from the extracts based on comprehensive analytical characterization by multiple analytical techniques. Methods The plant samples were extracted by sequential maceration using n-hexane, ethyl acetate, methanol, and distilled water. The compounds were isolated using a combination of chromatographic techniques and their structures were elucidated by multiple spectroscopic techniques. The antibacterial and antifungal activity determination of the isolated compounds was carried out using an agar well diffusion and potato dextrose assay against Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Candida albicans, and Aspergillus flavus while the antioxid...

Journal of Ethnopharmacology, Mar 1, 2015

Ethnopharmacological relevance: The plant Neoboutonia macrocalyx has been reported in traditional... more Ethnopharmacological relevance: The plant Neoboutonia macrocalyx has been reported in traditional medicine to be used in the treatment of malaria. Aim of the study: To study the in vitro antiplasmodial activity of compounds from the stem bark of N. macrocalyx. Materials and methods: Compounds were extracted and purified from stem bark of N. macrocalyx and their structure identified and confirmed by spectroscopic methods. The crude ethylacetate extract, aqueous extract and the isolated compounds were evaluated for antiplasmodial activity against the chloroquine sensitive Sierra Leone I (D6) and chloroquineresistant Indochina I (W2) strains of Plasmodium falciparum. Results: Chemical investigation of the ethyl acetate extract of N. macrocalyx bark resulted in the identification of one new diterpenoid; neoboutomacroin (1) in addition to the four known compounds which included, a phenanthrene; 3,6-dihyroxy-1,7-dimethyl-9methoxyphenanthrene (2), a sterol; 3-O-Acetyloleuritolic acid (3) and two diterpenoids; simplexin (4) and montanin (5). Compounds 1 and 5 displayed good antiplasmodial activity of IC 50 values less than 10 μg/mL against both strains. However, all the compounds tested displayed high cytotoxic activity against MRC5 cell line with IC 50 less than 10μM. Conclusions: Despite an indirect in vitro antiplasmodial activity of some compounds isolated from the stem bark of N. macrocalyx, the identification of these bioactive compounds indicates that they may play a role in the pharmacological properties of this plant.

European journal of medicinal plants, Mar 16, 2021

This study was done to document medicinal plants used in the management of dermatological disorde... more This study was done to document medicinal plants used in the management of dermatological disorders. Documentation of plants is important for conservation especially of rare and endangered plant species. The study was done in Buyende and Kayunga districts in Uganda, between April and July 2017. Data was obtained using semi-structured questionnaires and group discussions, performed on 63 respondents (33 females; 30 males) who were purposively selected because of their expertise in plant use. The study recorded 111 plant species that belong to 46 plant families for treatment of 30 skin disorders. The dominant life form was herb (41%), while leaves were the most used parts (59 %). Majority of plants (72%) were harvested from their natural habitats. Family Fabaceae contributed the highest number of species (20). Milicia excelsa was recorded to be threatened with extinction. The most cited diseases were skin rash (14%), wounds (12%), syphilis Original Research Article

Metabolites

Dalbergia melanoxylon Guill. & Perr (Fabaceae) is widely utilized in the traditional medicine of ... more Dalbergia melanoxylon Guill. & Perr (Fabaceae) is widely utilized in the traditional medicine of East Africa, showing effects against a variety of ailments including microbial infections. Phytochemical investigation of the root bark led to the isolation of six previously undescribed prenylated isoflavanones together with eight known secondary metabolites comprising isoflavanoids, neoflavones and an alkyl hydroxylcinnamate. Structures were elucidated based on HR-ESI-MS, 1- and 2-D NMR and ECD spectra. The crude extract and the isolated compounds of D. melanoxylon were tested for their antibacterial, antifungal, anthelmintic and cytotoxic properties, applying established model organisms non-pathogenic to humans. The crude extract exhibited significant antibacterial activity against Gram-positive Bacillus subtilis (97% inhibition at 50 μg/mL) and antifungal activity against the phytopathogens Phytophthora infestans, Botrytis cinerea and Septoria tritici (96, 89 and 73% at 125 μg/mL, re...

Molecules

There are high mortality and morbidity rates from poisonous snakebites globally. Many medicinal p... more There are high mortality and morbidity rates from poisonous snakebites globally. Many medicinal plants are locally used for snakebite treatment in Uganda. This study aimed to determine the in vitro anti-venom activities of aqueous extract and oils of Toona ciliata against Naja melanoleuca venom. A mixture of venom and extract was administered intramuscularly in rats. Anticoagulant, antiphospholipase A2 (PLA2) inhibition assay, and gel electrophoresis for anti-venom activities of oils were done. The chemical constituents of the oils of ciliata were identified using Gas chromatography-tandem mass spectroscopy (GC-MS/MS). The LD50 of the venom was 0.168 ± 0.21 µg/g. The venom and aqueous extract mixture (1.25 µg/g and 3.5 mg/g) did not cause any rat mortality, while the control with venom only (1.25 µg/g) caused death in 1 h. The aqueous extract of T. ciliata inhibited the anticoagulation activity of N. melanoleuca venom from 18.58 min. to 4.83 min and reduced the hemolytic halo diamet...

SINET: Ethiopian Journal of Science, 2008

This data set describes the medicinal plant species used in the treatment of snakebites in Acholi... more This data set describes the medicinal plant species used in the treatment of snakebites in Acholi, Teso and Karamoja sub regions of Uganda. It includes the how traditional medicinal practitioners (TMP) prepare and administer the medicinal plants, the parts used, type of snake envenomation treated and the frequency of citation. The data set also includes the biodata of the TMP.

African Journal of Pure and Applied Chemistry, Feb 28, 2009

... esculenta Crantz) Robert Byamukama*, Jane Namukobe and Bernard Kiremire Chemistry Department,... more ... esculenta Crantz) Robert Byamukama*, Jane Namukobe and Bernard Kiremire Chemistry Department, Makerere University, PO Box 7062, Kampala, Uganda. Accepted 5 February, 2009 ... pp. 471 - 553. Bua A, Otim-Nape GW, Byabakama B, Acola G, Baguma YK (1997). ...

Tropical Medicine and Health, 2022

Background Tuberculosis (TB) is one of the leading causes of death globally, and the rise in drug... more Background Tuberculosis (TB) is one of the leading causes of death globally, and the rise in drug-resistant forms of TB has become a significant threat. Subsequently, it is crucial to explore new, effective and safe anti-TB agents. This study aimed at conducting phytochemical screening, antimycobacterial activity, and acute toxicity of the selected plant species’ crude extracts to assess their toxicological potentials and efficacies against TB. Methods The aqueous and methanol/dichloromethane (DCM) (1:1) extracts of each selected plant species were subjected to phytochemical screening and antimycobacterial activity using microplate alamar blue assay. For acute toxicity, a single dose (2000 mg/kg) of the aqueous extracts was orally administered to each animal following the Organization for Economic Cooperation and Development (OECD) guidelines No. 425 and then observed for 14 days. The animals were closely observed on the general behavior and clinical signs of toxicity, and body weig...

European Journal of Medicinal Plants, 2021

Zanthoxylum leprieurii Guill. and Perr. (Rutaceae) stem bark is used locally i... more Zanthoxylum leprieurii Guill. and Perr. (Rutaceae) stem bark is used locally in Uganda for treating tuberculosis (TB) and cough-related infections. Lupeol (1), sesamin (2), trans-fagaramide (3), arnottianamide (4), (S)-marmesinin (5), and hesperidin (6) were isolated from the chloroform/methanol (1:1) extract of Z. leprieurii stem bark. Their structures were elucidated using spectroscopic techniques and by comparison with literature data. Furthermore, the extract and isolated compounds were subjected to antimycobacterial activity. The extract exhibited moderate activity against the susceptible (H37Rv) TB strain, but weak activity against the multidrug resistant (MDR)-TB strain with minimum inhibitory concentrations (MICs) of 586.0 and 1172.0 μg/mL, respectively. Compound 3 (trans-fagaramide) showed significant antimycobacterial activity against the susceptible (H37Rv) TB strain (MIC 6 μg/mL), but moderate activity against the MDR-TB strain (MIC 12.2 μg/mL). Compounds 2, 5, 6, and 1 showed moderate activities against the susceptible (H37Rv) strain (MIC 12.2-98.0 μg/mL) and moderate to weak activities against the MDR-TB strain (MIC 24.4-195.0 μg/mL). This study reports for the first time the isolation of compounds 1 to 6 from the stem bark of Z leprieurii. trans-Fagaramide (3) may present a vital template in pursuit of novel and highly effective TB drugs.

Supplemental material, sj-docx-1-npx-10.1177_1934578X211035851 for Antimycobacterial Activity of ... more Supplemental material, sj-docx-1-npx-10.1177_1934578X211035851 for Antimycobacterial Activity of the Extract and Isolated Compounds From the Stem Bark of Zanthoxylum leprieurii Guill. and Perr. by Benson Oloya, Jane Namukobe, Matthias Heydenreich, Willy Ssengooba, Bernd Schmidt and Robert Byamukama in Natural Product Communications

Additional file 1: Appendix 1. Ethnobotanical data on the medicinal plant species used in the stu... more Additional file 1: Appendix 1. Ethnobotanical data on the medicinal plant species used in the study areas.

There are high mortality and morbidity rates due to poisonous snakebites globally with sub-Sahara... more There are high mortality and morbidity rates due to poisonous snakebites globally with sub-Saharan Africa having some of the highest cases. Uganda is one the tropical countries with many poisonous snakes causing serious health hazards. Antivenin serum is the only remedy available to treat snakebite victims successfully to date. Infusion of antivenin drugs may lead to adverse reactions ranging from severe itching of the skin, hives to potentially serious allergic reactions. Due high cost and lack of antivenin drugs in residents of rural communities in Uganda, herbalists use different herbal formulations to treat snakebite victims. This study was conducted to search of a cheap, readily available and easy formulate remedies for treating snakebite victims. Root bark of Toona ciliata which is one of many plants used by herbalist was chosen for analysis of its anti-venom potentials. The aqueous extract of T. ciliata was tested for anti-venom potential against forest cobra (Naja melanoleuc...

Natural Product Communications, 2021

Zanthoxylum leprieurii Guill. and Perr. (Rutaceae) stem bark is used locally in Uganda for treati... more Zanthoxylum leprieurii Guill. and Perr. (Rutaceae) stem bark is used locally in Uganda for treating tuberculosis (TB) and cough-related infections. Lupeol (1), sesamin (2), trans-fagaramide (3), arnottianamide (4), ( S)-marmesinin (5), and hesperidin (6) were isolated from the chloroform/methanol (1:1) extract of Z. leprieurii stem bark. Their structures were elucidated using spectroscopic techniques and by comparison with literature data. Furthermore, the extract and isolated compounds were subjected to antimycobacterial activity. The extract exhibited moderate activity against the susceptible (H37Rv) TB strain, but weak activity against the multidrug resistant (MDR)-TB strain with minimum inhibitory concentrations (MICs) of 586.0 and 1172.0 μg/mL, respectively. Compound 3 (trans-fagaramide) showed significant antimycobacterial activity against the susceptible (H37Rv) TB strain (MIC 6 μg/mL), but moderate activity against the MDR-TB strain (MIC 12.2 μg/mL). Compounds 2, 5, 6, and 1...

Natural Product Research, 2021

Extracts from Securidaca longipedunculata showed antiplasmodial activities against reference clon... more Extracts from Securidaca longipedunculata showed antiplasmodial activities against reference clones and clinical isolates using SYBR Green I method. A new benzophenone, 2,3,4,5-tetramethoxybenzophenone (1) was isolated and characterized along with seven known compounds: 4-hydroxy-2,3-dimethoxybenzophenone (2); 3-hydroxy-5-methoxybiphenyl (3), methyl-2-hydroxy-6-methoxybenzoate (4), benzyl-2-hydroxy-6-methoxybenzoate (5), 2-hydroxy-6-methoxybenzoic acid (6), 2,4,5-trimethoxybenzophenone (7) and 2-methoxy-3,4-methylenedioxybenzophenone (8). Compounds 1 and 2 showed ex vivo antiplasmodial activities (IC50 28.8 μM and 18.6 μM, respectively); while 5 and 8 showed in vivo activities (IC50 19.7 μM and 14.5 μM, respectively) against D6 strain. In a cytotoxicity assay, all the extracts (with an exception of the MeOH extract of the leaves) and pure compounds were not toxic to the normal LO2 and BEAS cell-lines, while the methanol roots extract (IC50 66.4 µg/mL against A549, and 77.4 µg/mL against HepG2), compounds 6 (IC50 22.2 µM against A549) and 7 (IC50 45.2 µM against HepG2) were weakly active against cancerous cell-lines.